PrimerArray® Analysis Tool For Embryonic Stem Cells
Contents
1. 2 OK Microsott fficg ActiveX ctiven HL ctieeg 1 TE 0 URL FEF FF DO array calculatinn tonl wd2 0x ee ed 2 http wwwr takara bio coJp 7 IdRAR4 Tel 0 543 6116 Fax 0 5483 19 http www takarga Dio CO jp2. Housekeeping Gene Primer Set 3790 3791 3792 deNorm manual http medgen ugent be lt jvdesomp genorm geNorm_manual pdf Accurate normalization of real time quantitative RT PCR data by geometric av eraging of multiple internal control genes Vandesompele J De Preter K Pattyn F Poppe B Van Roy N De Paepe A Speleman F Genome Biol 2002 Jun 18 3 7 RESEARCH0034 Epub 2002 Jun 18 http wwwr takara bio coJp 4 6 Fold Difference 3D profle Fold Difference Control Sample 1 Test Sample Fnld Difference expressinnleyel 5 We nample ample nample nample An1 CTNNB1 9 1 100E 00 149E 0 An2 FLTI 100E 00 AH3 IFN1 100E 00 AH LAMAl 1nDE OD AI5 PDX1 100E 00 AG IPTEN 1E HH AH7 IGGL9 100E 00 AUH 100E 00 AH 1HUE n hin IGGR7 100E 00 Scatter plot Control Sample Scatter plot
3. PCR lt 1 PCR 0 4 6 B HO 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 1D 12 14 16 18 20 22 24 26 28 30 32 24 36 38 40 Cycles Cycles Threshold PCR Log scale
4. V201212
5. IdRaRa PrimerArray Analysis Tool For Embryonic Stem Cells V201212 PrimerArray Analysis Tool for Embryonic Stem Cells PrimerArray Embryonic Stem Cells PH016 PN016 1 PCR Ct AA Ct PrimerArray Analysis Tool for Embryonic Stem Cells Microsoft Offce Excel OS Microsoft Offce Excel Windows XP operating System Microsoft Office Excel 2003 Microsoft Office Excel 2007 http www takara Dio CoJp Dprimerarray_tool 2 1 Ct PCR Ct
6. Primary Curve 2 4 BB 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycles Threshold http wwwr takara bio coJp 2 2 Ct Ct PCR 3 Ct Microsofte Office Excel CSV PCR PCR Omit PrimerArray Analysis Tool for Embryonic Stem Cells I PrimerArray Analysis Tool for Embryonic Stem Cells AACt 1 PrimerArray Analysis Tool for Embryonic Stem Cells PrimerArray Analysis Tool for Embryonic Stem Cells PrimerArray
7. C Scatter Plot Samh Sample TNNB1 1 E 02 1 E 01 1 E 00 1 E 0 1E 02 2 TT 0 9 1 E 03 1 E 04 1E 05 1 E 05 1 E 04 1 E 03 1 02 TE D 1 E 00 1 E 01 1 E 02 ControlSample Hit a http www takara bio co jp 5 3D Profile Plate Test Sample Fold Difference Symbol Fold Difference 0 5 0 5 2 2 1 44E 00 3 08E 02 1 48E 00 4 2 09E 01 1 68E 01 2 48E 01 1 44E 00 8 01E 01 1 93E 01 5 79E 02 5 66E 01 4 51E 01 1 11E 00 9 27E 01 2 68E 01 9 79E 01 Category Reload Category Symbol FoldDifference Category ALL Clear em meel Category Embryanic Stem Cell Marker lt Transcription Factors for Embryonic Stem Cell Maintenance CA Sienal Pathway Genes for Pluripotency and Self Renewal
8. HKG Test Sample Control Sample Quantiy SDQ Quamtity SDQ PRT1 91GE 08 8GE 09 128E 07y 823E 09 IPGK1 487E 07 211E 08 8E 07 581E 0B ACTB 250E 05 3GG6E 06 329E 05 482E 06 a IGAPDH 351E 06 175E 07 590E 06 249E 07 TBP 156E 08 114E 09 282E 08 168E 09 417E D6 246E D 270E 06 247E D IPPIA 306E 06 0D0E 00 384E 06 0D00E 00 1 Normalization Factor HKG RNA
9. Cytokines and Growth Factars ther Embryanic Stem Cell Specific Genes Germ Layer Trophoblast Marker xtra Emhryanic Endodermal rophoblast Viseral Endodermal Parietal Endodermal rm n w ww ow Embryanic Stem Cell Differentiatinn Marker m lt Cn m COMMD3 FOXD3 CRABP2 IFITM1 NANOG 100 00000 1000000 mA mB 1ooooo mc 100 00 a a 1000 1 00 UL mF le mG 010 3 4 5 0 01 TestSampleData clear 2 Plate http wwwr takara bio coJp PrimerArray Analysis Tool for Embryonic Stem Cells Microsoft Offce Excel 2007
10. Analysis Toolfor Embryonic Stem Cells xls 2 Plate PrimerArray Plate Select PlateList _ ug Product Code FHO1E Primer Array Emhryanic Stem Cglls tHumans Prnduct Mame P Product Name WW 4 PH1E Primer Array Emhrwnnic Stem Cells hiouse 3 Control Sample Plate Select Control Sample ControlSampleData exp1 C exp2 D exp3 E Ct PCR t amp 10 A B GC D EE 2 ml We expi exp2 exp3 exp4 3 GTNNB1 4 EN 02m 265 2656 2655 5 FN IA03 2839 2843 2849 6 LAMA1 04 2051 2058 2056 7 EB 3104 8 PIEN 22 52 9 CCL2 10 ACTG1 11 _IAEP 12 CR7 13 BRIX 2363 2362 2372 14 GUSB 12 2387 2376 2404 15 GD84 0 3159 3154 313 16 EP9 B02m 2469 2509 2539 17 pH5 B03 3o78 83145 311 18 D 2 B04 Plate Select htt
11. p wwwr takara bio coJp 3 4 Test Sample Test Sample TestSampleData Control Sample Ct set sample data clear Ct cutoff Ct cutoff Ct 35 Ct 35 cutoff Ct cutoff value 5 Normalization Factor set sample data Normalization Factor normalization_factors HKG 1 NF value Normalization Factor
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