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Contents
1.2.
3. HLA HLA DNA 2003
4. NPO FCM MCM HLA
5. HLA HLA HLA 24 HLA CD34 colony assay HLA HLA HLA
6. HLA QCWS LIFT LIFT FCXM 13 HLA QC Luminex LABScreen Bit 1 NOME LABScreen 17 CSV Trimmed mean 13 QC Luminex Dt LABScreen HP
7. LAT 21S22 Control 1 1 1 1 1 1 2 Control 1 1 8 8 2 8 1 i 1 8 8 4 4 8 8 1 154 1 1 1 1 5 1 1 1 1 6 LAT 21S30 H Control 1 1 1 1 1 1 8 s Control 1 1 8 8 2 8 1 1 1 8 8 1 1 3 8 8 1 1E 1 1 1 1 5 1 1 1 1 6 AbScreen 21S31 N C OD x 2 N C P C FI OD 0 12 245 183 219 0 11 0 13 HLA Class I 8 8 a4 HLA Class II OD 17 18 095 01 009 0 08 8 1 1 20 MHC Vol 17 No 1 SH2103 Class 1 LCT AHG LCT Class II Flow PRA LABScreen 4 B 4 1 QCWS OCWS
8. HLA ix HLA HLA HLA HLA HLA dA
9. Bd CD34 CFU GM HLA HLA HLA HLA HLA HLA HLA HLA HLA HLA HLA HLA HLA HLA
10. class II histogram class II PE 865088232356 6 6 9 Q MHC Vol 17 No 1 25 FITC HP 20 Comp file 4 SA
11. 3 1 AHG LCT LIFT ICFA 3 3 C 6 1 5 42 Sample SH2101 AL A2 A24 B44 13 QC Al1 A24 FlowPRA LABScreen AHG LCT ICFA FCXM A2 B44 FCXM LIFT 8396 LCT AHG LCT ICFA
12. QCWS 2 FlowPRA Flow PRA 16 MHC Vol 17 No 1 FlowPRA LABScreen 2 1
13. Adsorb out SH2104 single antigen 13 6 Adsorb out Mixed PRA 3 2 single anti gen 1 HP No 47 Adsorb out 6 7 HP No 46 LABScreen single antigen SH2104 HLA non HLA HP No 46 4 3 HLA single antigen
14. 3 AbScreen LAT f b ELISA HLA 5 LAT AbScreen MHC Vol 17 No 1 79 3 LAT One Lamb da 2 AbScreen Biotest 1 LAT 1 Ab Screen 5 Clas I SH2101 SH2102 SH2103 SH2104 Class II AbScreen SH2101
15. PN PCR B HLA A B C DRB1 4 DQBI DPB1 13 QC DNA Luminex SSO 2 QCWS 58 24 3 M 3 3 1 1 HLA class 1 1 2 HLA class II 1 3 HLA class I amp II D050 4 1 4 P N f PCR 41 1
16. 13 QC 3 1 HLA I 1 1 1 2 HLA 3 2 SH2101 2104 2 1 2 2 SH2104 36 4 70 HLA non HLA CX 4
17. 13 HLA QC DNA SBT 1 SBT 9 1 2 SBT 2 7 SBT SSO SSP 2 EDI E e Df 1 8 AlleleSEQR abbott 1 SBTexcellerator QIAGEN 1 3 2 4 HLA C DQBI DPB1 10096 7 33 A 97296 B 86 196 DRB1 78 1 3 5 H2101 HLA A 1 DNA HLA B Abbott QIAGEN 2 B 3501 B 5101 9 5
18. H2103 21p02 pRBi 13070 DRBi 140301 DRBi 1401 06 39 IDRBI O405 DRBI 090102 DRB1 O101 07 DRB1 O701 2ID08 pR 130701 IDR 140301 DR 1401 06 DR 1406 39 54 DR 0405 IDR O101 07 DR 0701 21009 DRB1 130701 DRB1 140301 DRBI 1401 54 DRBI 1406 DRB1 0405 DRB1 O101 21 DRBI O70l ll 21D18 DRB1 130701 DRB1 140301 DRB1 1401 06 DRB1 1406 39 54 DRB1 0405 DRB1 O101 07 21 DRB1 O701 1 21D25 DRBI 130701 DRB1 140301 DRB1 1401 54 DRBI 0405 DRB1 O10101 DRBi O70 21D48 DRBI1 130701 DRB1 140301 DRBI 1401 06 21D53 pR81 1307 pRB1 1403 DR81 1401 06 DR81 1406 39 54 DRBI 0405 DRB1 O101 07 DRBi 070 21057 DRB1 130701 pRBi 140301 DRBI 140101 54 pRBi i amp 06 DRB1 040501 0503 DRB1 1406 39 54 IDRB1 0405 DRBi O101 07 IDRB1 O7O1 DRB1 070101 0102 X5 SBT A s o E202 H2103 H2104
19. SH2102 SH2103 Cw1 Cw1 Cw1 C SH2104 LotJOA Pc118 A11 B52 55 Cw1 12 rgG IgM IgG IgM 3 IgM IgM Lot JOA IgM
20. HLA Non HLA HLA A2 B44 ICFA LIFT FCXM HLA HLA HLA 0 uss4oS Vy A 0 33 VM
21. Lot 3 MHC Vol 17 No 1 29 5 Adsorb out non HLA 13 HLA QC Luminex WAKFlow HLA ICFA 1 WAKFlow HLA 1 amp II MR 1 1 WAKFlow HLA 72412958 7741138
22. 4 3 1 false positive false negative 2 ik 3 34 MHC Vol 17 No 1 13 HLA QC DNA Luminex 880 ox NPO HLA 1 3 QC 4
23. HP 5 6 histogram 4 11 15 19 IgG class 1 SH2104 21S08 SC class IL esG IgM SH2102 71 63 4 event class 10 000 IgM HP 7 histogram 9 3 2 SA 9 5 G1 4 GS 4 HP 21 24 plot 26 28 31 PE PESE plot Compensation
24. HLA A B C DRB1 2 22 HP 13 QC DNA 1 HLA A B C DRB1 1 30 24 2 6 HLA A B C DRB1 30 13 C 4 DRB1 2 96 0 90 H2104 A 0302 64 3 B 1302 78 696 Cw 0602 72 7 Cw 0702 63 696 1 2
25. 2 WAKFlow HLA II MR SH2101 WA4 Flow HLA MR iX SH2103 Pd12 DR12 DQ7 9 DPB1 0501 DR12 DOQ7 Pd12 Pd12 DR12 Pd12 2 ICFA 2 1 ICFA 2 2 SH2101
26. QCWS 26 MHC Vol 17 No 1 13 HLA QC FCM LIFT FCXM 1 LIFT 5 2 21S08 21832 FCXM 4 21S02 21S20 21S26 21S32 5 IgM 1 HLA class H 1 class I IgG LIFT score LABScreen Single Anti gen LABSA 15 rawdata 13 QC FCM LIFT FCXM
27. 4 4 RGB v 7e vos 12 1 2 4 C locus 4 HLA A B DR HLA A B C DR HLA A B C DR C locus HLA A B DR HLA A B C DR HLA A B DR HLA A B C DR 1 3 13 QC DNA
28. 4 SH2104 IgG class I 4 5 1 4 3 2 HP 12 SH2103 1 histogram 2 One Lambda 3 HP 13 histogram QCWS
29. Lot JOA 6 3 WAKFlow HLA iX 5 3 1 1 2 13 QC Luminex WAKFlow HLA TCFA 1 HLA SH2102 HLA Median 8 26
30. 4 2 SH2101 HLA A24 A2 B44 A24 A2 6 B44 8 35 LCT ICFA LIFT A2 B44 5 5 SERE s Tgs BI E 2 z 3 x O YHOI I1 YddAoH MHC Vol 17 No 1 13 43 Imtact
31. 10 MHC Vol 17 No 1 Z1 13th QCWS HLA DNA typing consensus results ID HLA A HLA B HLA C HLA DR A 2603 B 3501 04 24 B 5101 02 04 Cw 030301 Cw 140201 DRB1 130701 DRB1 140301 Heins A26 B35 B51 Cw9 Cw7 DR13 DR1403 A 2602 A 310102 B 1501 70 B 390202 Cw 0303 43 Cw 0702 29 50 DRB1 1401 54 DRB1 1406 iz A26 A31 B62 B3902 Cw9 cw7 DR14 DR14 A 020101 A 020601 B 270401 B 670102 Cw 0702 50 Cw 150201 DRB1 0405 DRB1 090102 e A2 A2 B27 B67 Cw7 DR4 DR9 4 0302 A 240201 B 0702 26 61 B 1302 18 Cw 060201 Cw 0702 50 DRB1 010101 DRB1 070101 NUN A3 A24 B7 B13 Cw6 Cw7 DR1 DR7 ID HLA DRB345 HLA DQA HLA DQB HLA DP DRB3 0101 DRB3 0202 DQA1 0501 05 09 DQA1 0503 07 DQB1 030101 DPB1 020102 ro DR52 DR52 nd nd DQ7 DPw2 daos DRB3 0202 DQA1 0101 04 05 DQA1 0503 07 DQB1 030101 DQB1 050301 DPB1 0501 DR52 nd nd DQ7 DQ5 DPw5 DRB4 0101 03 06 DQA1 0302 03 DQB1 030302 DQB1 0401 DPB1 0301 DPB1 0901 m DR53 nd nd DQ9 DQ4 nd nd DRB4 0101 03 06 DQA1 0101 04 DQA1 0201 DQB1 0201 02 04 DQB1 050101 DPB1 0501 DPB1 0901 E sadi DR53 nd nd DQ2 DQ5 nd nd Upper Allele type B 15010102N Lower HLA type 2 13th QCWS HLA antibody specificities consensus results
32. 3 FCXM 4 SH2101 NE A24 A2 A1 B44 0 cues HP BL c OR LIFT cutoff HP 6 Mp us b o x uS S N 232 0 cutoff LIFT cutoff MHC Vol 17 No 1 27 0 LIFT FCXM
33. Lot 2 DNA SSO Lot 2 SSO RELI 60 40 SSO DRB 30 DRB1 0405 H2103 DRB1 0405 30 2 8 DR4 PCR SSP 35 10 5596
34. Lot me 1 235 335 Dynal in RELI 14 5 1 MPH 1 9 0 mwocewEnGS INNO LiPA 1 0 0 i Total 16 5 1 22 u X2 HLA A HLA B HLA Cw HLA DRB1 HLA DRB345 HLA DQBI 22 22 22 22 7 22 22 22 15 22 2 22 32 MHC Vol 17 No 1 False positive False negative probe 1 3 2 False positive False negative probe 1 HLA A reference probe 21 2 8 HLA B refere
35. SSP Luminex DNA H2104 A 0302 1 6 16 796 6 2 3 A 0302 H2101 A SSP JPN MHC Vol 17 No 1 15 A 2601 02 03 A 2601 02 03 A 2501 2607 4301 1 4 2
36. Ilg 8 MHC Vol 17 No 1 HLA MIC QC CD QCWS 3 203 78 34 10 DNA QC 58 QC 36
37. 2 PCR 3 2 DRB1 3 3 B 2 locus 5 3 2007 2008 2009 4 5 6 3 2007 2008 2009 MHC Vol 17 No 1 35 Bd 5 class L class II P N PCR A cassI X y FAL class II 7 typing
38. HP 3 4 90 SSP SSO RELI 90 1 W NSUECHAS S 4 HP A d 5 38 SSO LABType 2 SSO 1 SSP SSO RELI SSO LABType 2 1 1 H2101 Cw 1402 No76 Positive Value 19 3696 No76 Cut off Value 20 Co Sls
39. 6 63 8 HP 2 QCWS QCWS QCWS DNA QC DNA 4 DNA ODNA HLA DNA HLA W QC S HLA C
40. 10096 HLA Class ILIgG SH2104 2 2 HLA Class I IgG SH2104 91 7 SH2101 SH2102 SH2103 100 2 4 LABScreenPRA QCWS PRA csv HLA Class I lsG SH2104 75 HLA Class II 100 FlowPRA screening 75 2 5 LABScreen single HLACIass I amp IL IgG 7 PIRE TCER DL HLA 9t 4 2 2 6 FlowPRA LABScreen single IgG 1 4 2 SH2101 6
41. 2 LABScreen Mixed 2 1 Mixed 17 7 Mixed HLA class 1 II HLA Visual NBG Ratio cut off 2 2 1 HP No 4 11 2 3 28 MHC Vol 17 No 1 NBG Ratio cut off Ratio HP No 12 3 LABScreen PRA 3 1 PRA 17 3 Mixed
42. o9 GO G ov O9 eu DNA apes Flow PRA AbScreen LAT DNA B DNA FCM D FlowPRA 2 AUS bz LIFT FCXM Luminex Lab Screen Boi MHC Vol 17 No 1 9 WAK Flow 1ICFA Bg 3 DNA D DNA Luminex SBT Luminex SSO
43. 1 90 100 SBT Luminex 100 Luminex 99 6 Luminex SSP 84 196 90 SSP SSP SSP 2 4 A B DR A B C DR 100 1 Luminex MHC Vol 17 No 1 27 2 10096 AB DR A B C
44. 10096 97 E HLA A24 A26 BS1 3 SBT Luminex SSO iX SSP SSP H2104 A 0302 28 6 7 2 1 2 Luminex 100 SSP 96 4 4 xix uM cR d REC I ERG 4 4 0 1 96 0 7 X 1 5 0 1 12 MHC Vol 17 No 1 16 14 SSP DNA
45. HLA ambigu ity 2003 MHC vol 10 3 160 162 WHO Nomenclature Com mittee for Factors of the HLA System 35 X U 1987 4 Marsh SGE et al Tis sue Antigens 65 301 369 2005 Human Immunol ogy 66 571 636 2005 Immunogenetics 32 107 159 2005 HLA 3 4 99 allele family HLA 2 233 O 5 E UL A 02 A 24 B 07 B 15 B 35 WHO Nomenclature Committee for Factors of the HLA System 2010 4 HLA 4
46. FCS00 FCS3 0 EACS Canto BD FCS FlowJo Tree Star USA 13 QC FCM FlowPRA HP 2 HP 2 20 11 7 2 Screening Test SC 1gM 6 Single Antigen SA 8 BD 12 BC 8 3 3 1 SC 4 IgG class 1 IgG class II SH2101 SH2103 8 1
47. PRA 4 LABScreen single antigen 4 1 single antigen 17 15 4 2 1 14 Lot 1 5 1 1 HP No 32 36 SH2101 2104 4 Lot 14 HP A74 FNo 37 45 No 48 51 4 000 1 000
48. 22 13 QC Luminex WAK7 ow HLA ICFA SH2101 A24 AHG LCT x LIFT iE A2 ICFA AHG LCT LIFT 1CFA HLA B44 1CFA Raw data SH2102
49. OIOIOIOIOIOIOIOIO co ET Er 1 II HLA GM jig IoIOIOIOOIOIOIOGI CoIOIO eo ololol oo CO olo fe oolololo o oloo oo olIOIOIO oo o 4 0 00 4 co ooOIO 6 6 G 6 G eo o0 ALB co co 0 4 o0 o o00 0 14 MHC Vol 17 No 1 13 HLA QC d 1 DNA 1 1 DNA A B C
50. 0 1 43 4 GR 3 33 HLA 34
51. HLA 68 4 4 1 1 DNA
52. 23 100 HLA HLA ambiguity 2003 DNA DNA HLA HLA 3
53. QCWS 4 HLA Class I 2101 2102 10096 2103 2104 94 4 66 7 HLA Class I 6 HLA Class II 2101 2102 2310096 2103 2104 87 596 93 896 HLA Class II 2103 1 2104 1 HLA 2 3 HLA HLA 10096 66 796 HLA QC 2104 high backsround high background
54. non HLA 4 4 1 QCWS QCWS DNA 13 QC
55. 3 2 3 PRAI 4 OCGBO2 Max SH2101 5096 SH2102 2103 25 HP No 17 23 PRA TI 1 Lot 12 Adsorb out HP No 24 28 3 3 11 QCWS PRA 11 7 Mixed single antigen
56. HP 2 HLA 2 HLA HP 1 4 2 AHG LCT LABSA rawdata 10 000 HLA A B SH2101 A24 SH2102 B7 B13 B27 B48 B60 B61 SH2103 0 Cw1 AHG LCT LABSA raw data 10 000 LIFT 1 LABSA raw data 3 000 3 000 HLA LIFT LABSA O rawdata 23 1 000 LIFT SH2104 LABSA rawdata 3 000 HLA LIFT HLA LIFT HLA LABScreen FlowPRA HLA LIFT LABScreen F
57. SH2102 A66 FlowPRA LABScreen FlowPRA A 6601 f LABScreen A 6601 A 6602 2 A24 4 4 5 High Background One Lambda Histocompatibility Work shop Absorb Out QCWS
58. HLA http hla alleles org data txVNomenclature_2009 txt 13 HLA QC FCM FlowPRA 3 MEIN LUE AUR bc 1 FlowPRA FCS LABScreen CSV FCS BC FCS BD file converter file converter FCS2 0 FCS3 0 FCS00 BC
59. RELD SSP A B DR 40 C 20 C 2 HP 2 1 30 SSO RELI 14 RELI 2 SSO SSP SSO SBT SSO RFLP 1 A B DRBI DRB3 4 5 14 3
60. 13 QC 13 QC DNA DNA Luminex SBT SSO http square umin ac jp JSHI QCWS QC DNA HLA Cii 24 MHC Vol 17 No 1 A Ae 4
61. HLA SBT HP QCWS QCWS QCWS CDR 5 DNA DNA SBT SSO Ambiguity IMGT HLA Database Sequence Alignments based on Release 2 26 0 July 2009 HLA 2003 HLA DRB3 4 5 DQA 1 2
62. QC HLA QCWS 2101 2102 2103 2104 HLA HLA 13 QC 2 2 HLA 19 19 9 10 HLA HLA Class I 19 HLA Class II 16 HLA fX 10 22 MHC Vol 17 No 1 FlowPRA 21 LABScreen 33 WAK Flow 1596 1 7096
63. 13 QCWS 19 11 QCWS HLA DNA QC DNA QO HLA QC QC QCWS MHC 13 QCWS 21 1 21 3 6 68 172 20 2 QCWS 4 ES S 5
64. B7 B40 SH2103 Cw1 Cw14 1ICFA C A B SH2104 ICFA ICFA MHC Vol 17 No 1 31 13 HLA QC DNA SSO SSP HLA 1 SSO 1 1 58 22 38 SSO 1 Dynal RELI MPH INNOGENETICS INNO LiPA 1 RELI 14 SSO SSP SSO SSP RFLP 3 1
65. 32 2 WAKFlow HLA 1 MR SH2101 A24 A2 A42 A2 A2 30 MHC Vol 17 No 1 Median Index
66. HLA HLA MHC Vol 17 No 1 23 1 Minoko Takanashi Koki Fujiwara Hidenori Tanaka et al The impact of HLA antibodies on engraftment of unrelated cord blood transplants TRANSFUSION 48 791 792 2008 13 HLA QC DNA HILLA QOC 13 12 HLA HLA b
67. Micro SSP Micro SSP SSO Dynal RELD Micro SSP SSO LAB Type Micro SSP SSO Dynal RELD RFLP Micro SSP UniTray SSP SSO LAB Type 4 MHC Vol 17 No 1 33 ntt SSP au SSP 1k SSO SSO SSP 5 One Lambda Micro SSP lt SsP ABC DRDQ m ENe SSP JPN 8003 4004 5 SSP AB DR SSP ABDR 006 8007 4 SSP I Generic Typing Kit SSP 1L 8006 007 4 SSP II Generic Typing Kit SSP 2L 4006 4 SSP Allele Specific xxxxx SSP1 xx SSP2 xx 4 p X X X 1_ SSP HLA x Typing Kit SSP xxx 2 SSP Seramates B15 SSPSB15 ai 1 3
68. 2 2 1 H2102 H2103 H2104 HLA DRB1 H2102 iX DRB1 1401 54 DRB1 140101 54 DRB1 140101 DRB1 1454 4 6 4 36 MHC Vol 17 No 1 1 9 21Do1 SBT SSO SBT SSO SBT4SSO SBT SSO ClassI A B C We ClassII DRBI DQB1 DPB1 21D08 SBT SSO SBT SSO 21p09 SBT SSO BT SSO se 21D18 SBT 21D25 SBT4SSO SBT SSO 21D53 SBT SSO 21D57 SBT4SSO X2 lsBT SBT SSO SBT SBT S ap48 sS sT gc Lud MIT uii SBT4SSO SBT 1I SBT4SSO SBT SSO SSP SBT SS0 SSP SBT SSO SBT SSO RFLP SBT 11 12 ClassII AlleleSEQR SBTexcel
69. FlowPRA B 1502 LABScreen B 1502 B 1511 2 LABScreen MHC Vol 17 No 1 7Z B 1S02 20 1803 B 1511 FlowPRA 1 78 1 98 7 1 FlowPRA LABScreen single IgG SH2101 FlowPRA LABScreen A 0301 62 5 A 0301 78 6 A 3001 100 A 3001 85 7 A 6901 100 A 6901 73 3 A 8001 00 A 8001 1009 B 4501 100 B 4501 100 B 8201 100 B 8201 1009 2 FlowPRA LABScreen single IgG SH2102 LABScreen A 6601 A 6602 21802 437 9643 8 FlowPRA LABScreen jis s 284 11021 8 A 2601 75 0 Ax2601 100 i5 ias Ax3101 85 7 A 3101 92 9 Pisos E iem 2 A 6601 e 1003 A 6601 A 6602 00 isi T 44d B 3801 62 5 B 3801 80 0 2 ile 2 B 3901 m 1009 B 3901 85 7 oisi 2j 1689 B 6701 100 B 6701 1009
70. recombinant 4 FlowPRA 2 101 6 94 1 SH2102 6 4 A66 FlowPRA A 6601 LABScreen A 6601 A 6602 2 LABScreen A 6601 A 6602 FlowPRA 3 76 6 92 1 SH2103 3 4 FlowPRA 9 LABScreen 2 92 3 96 7 SH2104 1 B75
71. Luminex 1 PC negative control NC Luminex 2 1 LABScreen single IgG 21S36 21534 21832 21828 21824 4 21811 21S10 21S09 21S08 21S07 21803 21S02 U 21S15 NC PC ZNC PC 18172 9523 16 21936 Hs 10810 17 10242 3772 21934 rg 19 L 3 as24 12725 44 11461 15067 46 21S15 8 13614 m 2 4913 je SH m 31 B 14786 9306 21810 7 154 p 7411 1612 21800 Fs 6 9213 12906 E 21S07 Fm 9111 15012 37 21803 10028 56 T 59 10924 10716 2802 Mer ogg 5 1 1 L j i Aoc aDUU 0 Bod0s 0900 000 DROP CT6000 0 2000 4000 6000 8000 10000 12000 14000 16000 Trimmed Mean HLA Class 1 Trimmed Mean HLA Class 28 FlowPRA FlowPRA LABScreen single
72. Ro x P P Ro E E oZ T7 o go oo 9 9 99 g Cw7 MICA MICA sai S E E Ee E BE 8 8 8 8 Gg a dre LS SA 8 8 8 8 8 4 4 4 4 4 4 2 2 8 8 8 8 8 8 8 8 8 8 4 8 8 FP SA 8 8 4 4 4 2 2 4 1 4 8 8 4 8 8 8 8 8 8 WAKFlow 8 8 4 2 8 2 4 8 8 8 8 8 ICFA 8 8 1 i 1 1 4 8 8 LIFT 8 4 4 2 1 1 AHG LCT 8 4 4 ja 1 1 LCT 8 iuo 1 1 MPHA 8 i L4 s 1 1 mo co 9p og ooo g 7 9 97vuooi o 9o O oo w ooo gt MICA MICA SH2102 8 8 EE G R T2 8 3 8 8 8 23 8 8 S uv 2239 MG dM LS SA 8 B B8B S8 8 58 B B B8B 8 B 38 8 8 8 8 8 8 8 4 4 4 4 4 8 4 4 2 2 1 FP SA 8 8 8 4 8 8 8 8 8 4 8 4 8 8 4 8 8 4 4 4 4 1 1 4 2 2 2 WAKFlow 8 8 8 8 8 2 2 2 2 2 ab ca ICFA 8 8 8 8 8 nd 4 4 4 4 BS 8 8 8 8 8 8 4 1 2 2 2 2 2 8 8 8 8 8 4 1 1 2 2 2 1 1 e 8 8 2 2 1 1 1 1 1 1 1 1 B 8 8 8 8 L2 2 4 _ 2 Em o9 ww mw gm w molo 2 o0 9 2 99 g 827 B61 MICA MICA z o SH2103 NN 2 amp c i SS 9 IgM IgM IgG IgM LS SA 8 8 8 8 4 4 4 4 4 4 4 4 4 2 8 8 4 8 4 8 8 FP SA 8 8 4 2 4 4 4 4 4 4 1 8 8 8 WAKFlow 4 1 1 3 1 1 T 1 1 1 4 1 1 ICFA 8 8 2 1 1 1 1 1 1 LIFT 8 8 1 t 1 1 1 1 i AHG LCT 8 2 i 1 1 1 i18 1 x LCT 8 1 13 1 1 d 1 1 MPHA 8g 2 1 b i i i Jj i Sg Sg 2 v MICA MICA SH2104 ij 2 o IgG IgM LS SA 8B 4 4 32 4 8 FP SA 4 4 WAKFlow i 1 ICFA i 1 LIFT 1 1 8 Strong positive LS SA LABScreen Single Antigen AHG LCT 1 1 4 Weak or several positive FP SA FlowPRA Single Antigen LCT 1 1 1 Negative MPHA i BS Nodata MICA using LABSc
73. DRB1 2 4 4 13 QC DNA HP 1 2 40 3 3 6 28 17 8 2 13 24 1 3 1 HP 1 40 SSO Luminex
74. Dynal RELI MPH INNO LiPA HLA 2 18 6 1 1 12 Dynal RELI HLA A 48probe kit 18 43probe kit 2 Lot 9 HLA B 61probe kit Lot 11 HLA Cw 5 37probe kit Lot 4 HLA DR 54probe kit 19 60probe kit 1 Lot 13 H2101 H2102 DR3 11 13 14 7 HLA DQ 2 41probe kit Lot 2 Lot Lot RELI
75. 5096 FlowPRA LABScreen FCM HLA 13 HLA QC BU EGGESNOD 2 1 DNA 1 1 DNA 23 13 3 4 3 HLA A B C DRBI 2 4
76. DR 3 C locus SBT Luminex A B DR A B C DR 10096 Luminex 2 C 1 SSP RELI SSP 0 Luminex 4 SSP C locus C locus C locus 2 2 1 13 QCWS 3
77. P N 1 1 locus A DRB1 B C locus 2 PCR 1 2 PN locus 42 1 P N 2 1 locus 1locus A B C DRB1 2 PCR 2 2 P N locus P N 4 locus DRBI 43 1 P N 3 1 HLA A B C HLA DRB1 DRB1 locus
78. 2 2 JPN ABDR 1L2L Lot False positive False negative HLA H2101 B 5101 H2104 A 0302 H2104 B 1302 H2101B 5101 SSP ABDR 4G False negative 22 SSP 1B IF False negative H2104 A 0302 ABDR 1D False negative H2104 B 1302 JPN SSP H2101 A 2603 A 25
79. FlowPRA Screening HLAClass IgG SH2104 SH2101 SH2101 SH2103 2310096 SH2104 73 75s SH2101 48 396 90 8496 SH2102 50 2096 92 5396 SH2103 7 4096 48 6096 SH2104 0 4096 9 7096 HLA Class II IsG SH2102 1 10096 SH2101 41 1096 86 60 SH2102 0 30 71 63 SH2103 6 60 74 70 SH2104 0 20 2 80 HLACIass I IgG SH2102 71 6396 22 FlowPRA single HLACIass I amp IL IgG 3 HLA 4 15 2 3 LABScreen HLA Class IgG SH2104 3
80. MHC Vol 17 No 1 7 13 HLA QC 13 HLA QC QCWS 3x a EI QCWS ARRHEZIP OHES 3 RUSO 2 9 7 1 HLA QC QCWS
81. bio 179 i6162 9 21S15 144 14917 8 21S24 172 12042 8 21S28 222 15497 8 21832 142 15019 8 21934 289 12707 8 21835 175 17511 8 21836 152 12388 8 Trimmed Mean 3 FlowPRA LABScreen single lgG SH2103 FlowPRA Cw 1802 DRB1 1501 DRB1 1502 10090 B 4201 75 0 60 0 DRB1 1501 DRB1 1502 LABScreen Cw 1802 78 6 B 4201 64 3 83 3 18 MHC Vol 17 No 1 4 FlowPRA LABScreen single IgG SH2104 B 1502 FlowPRA 6 LABScreen B 1502 BA 1511 21502 1045 8 21S03 1803 8 21S07 727 107 1 LABScreen 21S08 1348 212 4 6 7 B 1502 B 1511 71 4 21S09 20 23 1 21510 284 110 1 21811 678 147 8 21815 438 78 1 21S24 1026 109 8 21928 398 104 1 21932 787 134 1 21834 688 1 21835 916 126 1 21536 622 104 1 2 7 LABScreen single IgG LABScreen Baseline Trimmed Mean HLA IgG 2 tgsG positive Ratio Scoring control PC 1 Trimmed Mean NC
82. lerafor H2103 DRB1 0405 DRB1 040501 0503 DRB1 040501 DRB1 040503 6 1 4 H2104 DRB1 DR 3 6 SBT SBT SBT MHC Vol 17 No 1 37 X4
83. lowPRA 0 5 3 23316 BE HLA LIFT HLA LABScreen FlowPRA HLA SH2104 LABSA HLA LIFT LABSA HLA 1 LIFT HLA HLA 1 HLA 1
84. nce probe 29 61 2 6 HLA Cw H2104 probe 21 30 056152389 4 False nega tive HLA DRB1 H2103 I2thQCWS probe 30 DR4 probe 24 442 probe 430 2 SSP 2 1 HLA 38 18 31 SSP 3 One Lambda Micro SSP invitrogen UniTray 3 SAGE Kit 1 SSP SSP 4 5 One Lambda Micro SSP JPN ABDR SSPIL SSP2L HLA 14 5 9 1
85. reen Mixed MHC Vol 17 No 1 11 13 HLA QC MURS 1 BuU 25 HP 13 QC 2 DNA 2 1
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