pT7-IRES His-N DNA - Clontech Laboratories, Inc.
Contents
1. Bei CHEE EMCV IRES 10mM Tris HCl pH8 0 1mM EDTA my v2012020a mcs Nco EMCV IRES Nhe His Tag Factor Xa Nde Sac Xho 5 TAACGT TAATATGGCCACAACC ATG GCT AGC CAC CAT CAC CAT CAC CAT ATC GAA GGG CGC CAT ATG GAG CTC CTC GAG 3 lt ATTGCA ATTATACCGGTGTTGG TAC CGA TCG GTG GTA GTG GTA GTG GTA TAG CTT CCC GCG GTA TAC CTC GAG GAG CTC Met Ala Ser His His His His His His lle Glu Gly Arg His Met Glu Leu Leu Glu Hinc ll BamH EcoR Spe Sal Pst Xbal End GGA TCC GAA TTC ACT AGT GTC GAC CTG CAG TCT AGA TAG GTAATC 3 CCT AGG CTT AAG TGA TCA CAG CTG GAC GTC2. AGA TCT ATC CATTAG 5 Gly Ser Glu Phe Thr Ser Val Asp Leu Gln Ser Arg uaz n o HEERS enen TaKaRa R DR
3. TG GTA GTG GTA GTG GTA TAG CTT CCC GCG GTA TAC CTC GAG GAG CTC Met Ala Ser His His His His His lle Glu Gly Arg His Met Glu Leu Leu Glu Hinc ll BamH EcoRI Spe Sal Pst Xbal End GGA TCC GAA TTC ACT AGT GTC GAC CTG CAG TCT AGA TAG GTAATC 3 CCT AGG CTT AAG TGA TCA CAG CTG GAC GTC AGA TCT ATC CATTAG 5 Gly Ser Glu Phe Thr Ser Val Asp Leu Gln Ser Arg pT7 IRES His N DNA Code No 3290 3 429 bp Size 20 ug Shipping at 20 C Store at 20 C 1 Dideoxy T7 EMCVIRES His Factor Xa polyA T7 2 2 EcoRI 1 APA Hind I 2 EE ET Lot No eee ee pT7 IRES His N DNA E e e S p E Is TM polyA T7 terminator Multiple Cloning Site Factor Xa Site His Tag a Human Cell Free Prot
4. ein Expression System EMCV 3281 IRES PT7 IRES His N DNA 3 429 bp Py pT7 IRES DNA T7 EMCVIRES His cMyc Factor Xa polyA T7 ColE1 ori pT7 IRES His N DNA ld His pT7 IRES His N DNA Human Cell Free Protein Expression System 3281 N His His His ens gt 17 EMCV RES RNA
5. ity 1 Confirmed to maintain the region from T7 promoter to T7 terminator by dideoxy sequencing method 2 Shown to be cleaved at a single site by EcoR and at two sites by Hind Ill Usage Protein expression using Human Cell Free Protein Expression System Vector map for pT7 IRES His N DNA T7 terminator polyA Multiple Cloning Site Factor Xa Site His Tag Amp EMCV IRES pT7 IRES His N DNA 3 429 bp Pr ColE1 ori Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements Chain length 3 429 bp v201202Da MCS Neo EMCV IRES Nhe His Tag Factor Xa Nde Sac Xho 5 TAACGT TAATATGGCCACAACC ATG GCT AGC CAC CAT CAC CAT CAC CAT ATC GAA GGG CGC CAT ATG GAG CTC CTC GAG 3 ATTGCA ATTATACCGGTGTTGG TAC CGA TCG G
6. pT7 IRES His N DNA Code No 3290 Size 20 ug Shipping at 20 C Store at 20 C x 2 years from date of receipt under proper storage conditions Lot No Concentration 0 5 ug ml Volume 40 ul Regarding protocol for protein synthesis please refer to the product manual for Human Cell Free Protein Expression System Cat 3281 Description pT7 IRES DNA series are expression vectors designed for Human Cell Free Protein Expression System Tag sequence such as His Tag or c Myc Tag Factor Xa cleavage site Multiple Cloning Site MCS polyA and T7 terminator are located at the downstream of T7 promoter and EMCV IRES There are vectors with some arrangements for the kind of Tag and the location pT7 IRES His N DNA is an expression vector including His Tag sequence Using pT7 IRES His N DNA together with Human Cell Free Protein Expression System Cat 3281 enables the synthesis of your target protein as N terminal fusion of His Tag Factor Xa cleavage site is inserted so that His Tag can be removed from the synthesized fusion protein Target gene cloned into MCS in frame is transcribed as RNA containing EMCV IRES under the control of T7 promoter By the effect of EMCV IRES designed to promote protein translation initiation efficient high level protein synthesis can be performed in Human Cell Free Protein Expression System Form 10 mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column Pur
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