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1. 1995 11 15 960 12 1 2 1 transforming growth factor LAT TGF 8 Il 1 40 5 Wistar Japan SLC Inc 22. binalization 2 X100 5 6 BrdU BrdU 24 29 3 13 5 48 13 4 BRO 1 BrdU 50mg kg BR
3. tL ERB binalization 9
4. BrdU Fig 4a BrdU 33 693 Fig 4b 24 BrdU Fig 1 Microscopic feature of the liver in rats treated with intraperitoneal administration of thioacetamide Liver cirrhosis is shown on the tissue H E staining X10 Fig 2 Automated image analysis of hepatic fibrosis index on Avan stained liver tissue a Input b Binalization c Calculation of hepatic fibrosis index m P ia a F a HR p5 Y F ti u TOA E a ie T PE y at ve 1 i ie a Li Nala ats RAPS Ti Tirip ar k Phe 4 x iiir at ier LA ee Pen a oe ap re Te Bi Fa ate aw gio Fi apie f HP a EF rte MA ee a tee a oe ATIE as at l ut i Pe e n i j 3 ET 34 694 28 77 53 61 45 39 11 42 48 11 13 16 60 0092202060 005 420 TOR 24 BrdU 7
5. 36 696 TGE g hepatocyte growth factor HGF TGF 8 3 TGF 8 TGF 2 Tee
6. BrdU BrdU DNA BAI BrdU BrdU S Hic BrdU S 24 13 OLR 48 13 4 Lic BrdU 24 48
7. HO 7 BrdU 24 48 BrdU N terminal propeptide of type III procollagen PIIP PIIIP 7 2 22
8. BrdU 24 r 0 50 p 0 048 48 r 0 53 p 0 037 Hote Fig 7 8 BrdU IV amp AAR 73 7 Fil 82 1 2 dD Fig 4 Immunohistological staining for BrdU of residua liver tissue in rats at 24 hr after partial hepatectomy X40 a rats treated with intraperitoneal administration of thioacetamide b doses of saline Ca Ww fa o as nie ain _ a W jea C 1996 3 Fig 5 Correlation betwe
9. 7 19 12 22 1 5 C 5545 0 b amp THB Lz HIV I A 7 EA CE 2 PEU 3 30 4 200mg kg 3 E 10 ip 10 4 32 692 Higgins 2 3 10 3 DS 1 1 26 9 UE Given kas 5 D 20
10. multilobular nh Fig 1 2 Fig 2a b c 4 36 35 61 17 04 7 64 z 0 21 0 70 0 51 0 16 p lt 0 001 Fig 3 3 FRAFO BrdU BrdU
11. 1996 3 Shimada HPT Pugh s score ICG R15 Fibrosis score 23 D TGF g C0 95 1995 4 A 46 1995 7 x BR 1 The liver cancer study group of Japan Pri mary liver cancer in Japan Clinicopathologic features and results of surgical treatment Ann Surg 211 277 287 1990 2
12. 10 34 805 813 1993 3 13 84 89 1980 4 wis dec ome 16 40 45 1989 5 26 5 18 1987 l 6 3 723 741 1984 7 Higgins GM Anderson RM Experimental pathology of the liver I Restoration of the liver of the white rat following partial surgical removal Arch Pathol 12 186 202 1931 8 MEAT 7 PY eee Med Technol 5 1022 1025 1977 9 3 I 5 1974 10 5098 R 1992 p243 247 11 Nakatsukasa H Nagy P Everts RP et al Cellular distribution of transforming growth 12 13 14 15 16 17 18 19 20 21 22 23 37 697 factor 81 and procollagen types I HI and IV transcript
13. 1 MOR Jimenez picric acid sirius red 29 35 ZODRA fast green sir iusred 1 IV HA I IL IV LO
14. 3g HE HE 10 10 15 5 G 15 5 OTR 15 Color Image Processors SPICCA II Avionics PC 9801FS NEC 1Image Command 5098 Avionics t
15. 29 3 691 698 1996 2 PRS Fak TGF Wistar 2 3 24 48 BrdU BrdU r 0 64 0 60 BORO r 0 50 0 53 BrdU
16. p lt 0 05 Key words hepatic fibrosis liver resection liver cirrhosis hepatic regeneration activity automated image analysis L 4 FT ADAG HEL RRR EO BRE LO ER ROI _EDS 90 L 2 9
17. BrdU BrdU 7 BrdU HE 24 48 BrdU 8 PC 9821As NEC SPSS for Windows SPSS Japan Inc 2 Student t 2 Spearman p lt 0 05 LL 1 1996 3
18. 38 42 1985 Tanaka Y Minato Y Hasumura Y et al Evaluation of hepatic fibrosis by serum proline and aminoterminal type III procollagen peptide level in patients with chronic liver diseases Dig Dis Sci 31 712 717 1986 Plebani M Burlina A Biochemical markers of hepatic fibrosis Clin Biochem 24 219 239 1991 Shimada M Matsumata T Adachi R et al Estimation of degree of liver cirrhosis using a fibrosis score a multivariate analysis of clini cal parameters and resected specimens He patogastroentrology 41 177 180 1994 38 698 29 3 The Grade of Hepatic Fibrosis as a Predictor of Regeneration Activity after Partial Hepatectomy to Liver Cirrhosis in the Rat Yuichi Hatakeyama Toshiyuki Ono and Rikiya Abe The Second Department of Surgery Fukushima Medical College Liver resection is the most effective method of therapy for hepatocellular carcinoma We need accurate information about the liver function before liver resection because most patients with hepatocel lular carcinoma have associated liver cirrhosis in Japan It has recently been reported that Transforming Growth Factor TGF 8 which promotes liver fibrosis and suppresses the growth of hepatocytes is related to the development of liver cirrhosis So we focused our attention on liver fibrosis resulting from TGF 8 and investigated whether the extent of h
19. 92 59 80 32 61 14 15 48 5 09 15 17 9 56 2 59 Hore 4 BrdU BrdU 24 r Fig 3 Comparison of hepatic fibrosis index between rats treated with intraperitoneal admin istration of thioacetamide and saline hepatic fibrosis index 0 0 35 A 30 thicacetanide 25 E saline 20 F j p lt 0 001 15 i 10 lt a 29 3 0 64 p 0 003 48 r 0 60 p 0 005 Fig 5 6 H E 2 BORE SL 7 2 7
20. BAY 70 3g BrdU 0 3 H O0 0 1N 30 2N 60 0 1M RY Piz kom ji BrdU DAKO ft 20 4 C DAKO th 300 37 C 60 DAKO 37 C 60 3 3 diaminobenzidine tetra hydrochlolide DAB S ABC MMayer s Hematoxylin 2 000
21. 35 695 Fig 7 Correlation between hepatic fibrosis index and BrdU labeling index of rats with histopath ologically liver cirrhosis hepatic fibrosis index gt 7 at 24hr after partial hepatectomy BrdU labeling index 60 50 thioa cetami de 40 liver cirrhosis 30 t 0 50 p 0 048 z Y 1 20X 49 05 20 a 10 9 0 4 0 5 10 15 20 25 TY hepatic fibrosis index Fig 8 Correlation between hepatic fibrosis index and BrdU labeling index of rats with histopath ologically liver cirrhosis hepatic fibrosis index gt 7 at 48hr after partial hepatectomy BrdU labeling index 20 a thioacetamide liver cirrhosis r 0 53 p 0 037 Y 0 18X 13 32 10 20 a0 40 hepatic fibrosis index 6 DD S
22. en hepatic fibrosis index and BrdU labeling index of all rats at 24hr after partial hepatoctomy BrdU labeling index 4 70 60 b hioacetamide saline r 0 64 p 0 003 Y 1 18X 49 05 0 5 10 15 20 25 hepatic fibrosis index Fig 6 Correlation between hepatic fibrosis index and BrdU labeling index of all rats at 48hr after partial hepatectomy BrdU labeling index 20 thioacetamide H saline r 0 60 p 0 005 Y 0 17X 13 10 0 10 20 30 40 hepatic fibrosis index risk OB MH Rmax 1
23. epatic fibrosis hepatic fibrosis index might be correlat ed with hepatic regeneration activity after partial hepatecotmy in rats with thioacetamide induced liver disease Two groups of male Wistar rats 5 wk old 10 to 30 group were treated for 10 wk by weekly intraperitoneal administration of thioacetamide 200 mg kg 3 times wk or saline All rats then under went 70 hepatectomy under pentobarbital anesthesia Hepatic regeneration activity was determined 24 and 48 hours after parital hepatectomy by means of bromodeoxyuridine incorporation into DNA The hepatic fibrosis index was semiquantitatively calculated at the time of partial hepatectomy by automated images analysis on Azan stained liver tissue A significant inverse correlation was found between the hepatic fibrosis index and DNA synthesis in all rats thioacetamide treated and saline treated 24 and 48 hours after partial hepatectomy r 0 64 and 0 60 respectively p lt 0 05 and also an inverse correlation existed even in the rats with treated thioacetamide r 0 50 and 0 53 at 24 and 48 hr respectively In conclusion it is considered that the preoperative quantitation of hepatic fibrosis by automated image analysis will enable us to predict hepatic regeneration activity after cirrhotic liver resection Reprint requests Yuichi Hatakeyama The Second Department of Surgery Fukushima Medical College 1 Hikarigaoka Fukushima 960 12 JAPAN
24. s in carbon tetrachloride induced rat liver fibrosis J Clin Invest 85 1833 1843 1990 Macintosh EL Gauthier T Pettigrew NM et al Hepatic fibrosis as predictor of hepatic regeneration activity after partial hepatectomy in the rat Hepatology 17 307 309 1993 MEE TGF 10 1892 1897 1992 PEF 34 212 218 1993 Jimenez W Pares A Caballeria J et al Measurement of fibrosis in needle liver biop sies Evaluation of a colorimetric method Hepatology 5 815 818 1985 HEAR THE BrdU S E 27 1632 1632 1986 Sato S Nouchi T Warner TM et al Liver fibrosis in alcoholics JAMA 256 1471 1473 1986 Savolainen ER Goldberg B Leo MA et al Diagnostic value of serum procollagen peptide measurements in alcoholic liver disease Alco hol Clin Exp Res 8 384 389 1984 Rojkind M The blue glass and the predictive value of serum amino terminal propeptide of type III procollagen as a marker of liver fibrosis Hepatology 4 977 978 1984 Galambos MR Collins DC Galambos JT A radioimmunoassay procedure for type III procollagen its use in the detection of hepatic fibrosis Hepatology 5
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