ダウンロード - プリマハム
Contents
1. 3 000Xg 20 CE H E ub 1 1 A2. 4 5 4 F 20 6 5 BE Qg m1 50 25 12 5 6 25 3 13 1 56 0 78 0 Na uL 800 400 400 400 400 400 400 0 W NM gt gt gt uL oJ 400J 400J 400J 400J 400J 400 400 6 G 10 3
3. ng mL 1 1 2 3 4 5 6 7 8
4. A 96 M 89z X12 1 B 50 ng m1 1 mL 2 9 C An ER 10 D TMB 13 mL 1 E 0 5 M 0 25 M 13 mL 1 1 F 100 mL 1 A B C 24 G 0 100 mL 1 1 1 1 7 H A 10 50 mL H A 10 100 mL 1 I B 10 50 mL I B 10 100 mL 1 A J C 10 50 mL J C 10
5. 7 C 8 9 1 2 D H EE 1 g 50 mL 19 mL 90 110 3 cm pH6 0 8 00 pH
6. 1 50 pL 1000 pL 50 mL 2 ELISA I B 077886 3 4 Di 6 450 nm 2 450 nm 600 650 nm 7 4 Logistic 1 1 ELISA 2 20 25C 2 B 3 2 3
7. B jn 1 9 10 1 8C 2 3 1 ELISA ELISA
8. X Y 0 75 1 25 R 0 9 ug g 10 ng g 0 20 40 60 80 100 0 80 60 40 gt SN SN K PA 20 ng g 10 000 ug g 12 500 10 000 7 500 5 000 2 500 0 2 500 5 000 7 500 10 000 12 500 100 ug g uug g n n 0 9 0 9 26 0 87 0 979 45 ios o 997 FF 300 0841 TEL 029 842 4333 635 FAX 029 842 5216
9. 2 0 0 78 50 ng mL 100 pL 3 1 300 400 pL 5 4 2 C 1 hL 3 30 1 300 400 pL 2 D 100 nL 3 1 20 950 pL Rs 2 HH
10. 4 20 25C 3 5 4 ELISAT 6 ZN 7 8
11. 26 12
12. 100 pL 1 100 pL 30 100 pL 10 100 pL 450 nm 600 650 nm 1 450 nm 600 650 nm 1 4 Logistic 4 2 3 400 5
13. 100 mL 1 350 mL 1 C 500 mL 1 B 2 EE 2 0 78 50 ng mL ELISAT B 1 2 3 Ai 8
14. 1g 19 mL 12 pH6 0 8 0 3 000Xg 20 20 50 uL 1 E 0 0 78 50 ng7mL 100 5 10 4 E 100 pL 1 30 2
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