糖鎖固定化アレイご紹介資料
Contents
1. S SUMITOMO BAKELITE CO LTD S BIO NR N p lt m pa 7 HERA 33 SUMITOMO BAKELITE CO LTD 5 SUMILON S ll AFM 1 Control 2 TGF B 3 Erythropoietin 4 Histo2. S N gt gt N O 96well SUMITOMO BAKELITE CO LTD 5 SUMILON DSS MEEZIT 280A EAEk NEI 2Z54E 1 3 BS X1731 1 80 000 BS X1732 4 300 000 BS X1733 8 560 000 1 1 24 E 1 5 BS X1714 14 80 000 5 1715 4 300 000 BS X1716 8 560 000
3. BS X1717 1 15 000 1 BS X1720 1 10 000 2 SUMITOMO BAKELITE CO LTD 5 aa SUMILON Man Gic GalNAc GIcNAc Neu5Ac SUMITOMO BAKELITE CO LTD S BIO SUMILON 10 Heparin Or 11 2 O Desulfated Heparin 8 STn 9 T 12 6 O Desulfated Heparin 13 N Desulfated Heparin 14 N Desulfated reN Acylated Heparin 1 i B 4 4 B 3 CORSIE 15 Le 16 17 SLea 19 SLex Gal Gic GalNAc e or GIcNAc 19 Lac 20 a2 3 Sialyl lactose 21 a2 6 Sialyl lactose SUMITOMO BAKELITE CO LTD S BIO 22 a2 3 Sialy LacNAc 23 a2 6 9ialy LacNAc 24 Lacto N tetraose 25 Lacto N neo tetraose Man Gal Gic GalNAc GIcNAc 26 Atrisaccharide 27 Btrisaccharide 28 H O disaccharide SUMITOMO BAKELITE CO LTD S BIO SUMILON
4. 2934532236 SSSI ES ScanArray Lite PerkinElmer TR SUMITOMO BAKELITE CO LTD Sb SU SUMILON Alexa Fluor JS hH l x 10 ng mb 1h RT B m 0 25 mM m 0 1 mM asialo GM1 asialo GM2 SSEA 4hex P Forssman SSEA 4 tet B GM1 B ScanArray Lite PerkinElmer ul 1 Merritt EA et al 1994 13 745 753 2 Chinnapen DJF et al FEMS Lett 2007 266 129 137 SUMITOMO BAKELITE CO LTD S BIO 5 5 5 ils i s s s l ll ll l i i SUMILON Ajia HEEEL DRAA SRLS o c GM1a GM1
5. 5 Fuc NA4 N Glycan 10ug ml SUMITOMO BAKELITE CO LTD NA2F A2 96well plate type wi 25 2 lt p NA2 PHA L4 5 MAN 3 NA4 NA2F MAN 3 5 SUMILOIY 2 Glycosaminoglycan 96well plate type CHOR O N O N NHAC Dermatan Sulfate ldoA GalNAC To OR H NHR H NHR Sulfate GlcA GlcNH Heparin ldoA GIcNH SO R H or Ac or SO SUMITOMO BAKELITE CO LTD 9 5 b SUMILON 96well plate type 3 Heparin FGF2 ELISA 3 5 Heparin MW 5000 Heparin MW 10000 a Heparin High MW A450 SUMITOMO BAKELITE CO LTD 4 SUMILON S
6. Fw Man Gic GalNAc lll GIcNAc Neu5Ac SUMITOMO BAKELITE CO LTD S BIO SUMILON SAAN PAPARAZI Akn 9 GD1a 10 GD1b 11 GT1a 12 GT1c 13 GA1 asialo GM1 Man Gal Gic GalNAc Ll O GIcNAc 14 Fucocyl GM1 15 GA2 asialo GM2 SUMITOMO BAKELITE CO LTD 5 SUMILONY 535 2 lt 4 Bp 4 16 Gb3 antigen 17 Gb4 antigen 18 Gb5 55 B 4 B 4 19 Globo SSEA 3b 20 Globo 22 SSEA 4 tetraose 3 B 3 4 B 4 23 SSEA 4 hexaose 24 Forssman antigen Man Glc GalNAc GIcNAc SUMITOMO BAKELITE CO LTD SUMITOMO BAKELITE CO LTD 5 SUMILOIY 1 N Glycan 96well plate type 5 N Glycan 5 ConA ECA SNA LCA PHA L4 L4 10ug ml Gal
7. s bio sumibe co jp T 140 0002 2 5 8 TEL 03 5462 4831 03 5462 49835 SUMITOMO BAKELITE CO LTD
8. O 0 25mM x GM3 GT3 GM2 GD2 GT2 GM1a GM1D 0 1mM GD1a GD1b GT1a GT1c GA1 asialo GM1 Re omo x Fu cosyl GM1 GA2 asialo GM2 _Gb3 Pk antigen Gb4 antigen Gb5 SSEA 3 Globo H SSEA 3 Globo A Globo B SSEA 4 tetraose SSEA 4 hexaose Forssman antigen SUMITOMO BAKELITE CO LTD 5 5UMILON 1 2 1 4 5 SUMITOMO BAKELITE CO LTD S BIO Nb SUMILONF op 2h RT LT x Streptavidin Cy3 2 ug mL RT x AAL 40000 35000 30000 25000 E E 20000 15000 10000 5000 E ________
9. a o 1 7 z v gt K k 222 2 7 gt De 1 x N gt 5 0 GT2 GM1a GM1b GD1a gt 10 GD1b Pr Tp A Ed 11 GT1a 12 GT1c OO 13 GM1 ro 590009 14 fucosyl GM1 TO 15 asialo GM2 16 Sa e 17 Gb4 18 Gb5 19 Globo H 20 Globo 21 Globo 22 SSEA 4 tetraose 23 SSEA 4 hexaose E5 24 Forssman antigen 0 O g h Fi 4 2 5 Signal intensity X 10 1 2 3 4 5 6 7 8 9 10 1112 1314 15 16 17 18 19 20 21 22 23 24 Oda Kabura 1akagishi T Suzue Tominaga K Urano S Nagahama Kobayashi K Furukawa K Furukawa K Sakurai J Clostridium perfringens alpha toxin recognizes the GM1a TrkA complex J Biol Chem 287 39 33070 33079 2012 SUMITOMO BAKELITE CO LTD 5 SUMILON i i i ia m m
10. ne H1 S N 5 SUMILOIY 0000 eee l Nglycan 6 O e eeoeollCglycan e e e 0000 0000900 00000 Lewis OE EEEE JOSES OE EEEE JOSES 0000000000000 Grid marker x 28 0 5mg mL GAG Mglycan M9 2 A2 NA4 7 0 2mM N glycan Oglycan STn T 2 1 0mM other glycans Heparin Desulfated heparin 4 5 0 1mgmL GAG Lewis Lea Le SLea SLe 4 0 04mM N glycan Lac Lac a2 3Sialyl Lac 2 65 Lac 0 2mM other glycans o2 3Sialy LacNAc a2 6Sialyl LacNAc E A Lacto tetraose Lacto M neo tetraose 7 ABO Atrisaccharide B trisaccharide disaccharide 3 SUMITOMO BAKELITE CO LTD 5 SUNI LON 24 000 000 0060 000 006 0006 000 000 00O 000 000 000 SS 000 000 000 000 O O O O O O O O O O O
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