DNA すいすい -PF
Contents
1. DNA 1 1 v v 70 TE 10 mM Tris HCL 1 mM EDTA pH 8O pH 8 O 1 1 25 24 1 SIGMA Cat No P2069 7 3 DNA FF0 PF DNA 8 25 50 mg 1 5 ml2. SIGMA Cat No P2069 15 0OO rom 20 25C 10 5 DNA DNA DNA DNA DNA 4
3. 70 SIGMA Cat No P2069 15 000 rpm lt 20 25C 10 5 8 DNA FF0 PF PCR DNA DNA 141 bp PCR 18S rRNA 200 bp PCR DNA DNA or 1 2 3 4 5 6 EL ECE 2 141 bp 18S rRNA 200 bp 3 Agarose M Marker 5OO bp 4OO bp 2OO bp 10OO bp
4. 1 8 2 9 3 10 4 11 5 12 6 13 7 14 PCR PCR Buffer 1x 95C 2 min dNTPs O0 2 mM 94C 30 sec MgCb mM 6OC 30 sec gt 40 cycles Primer O6 uM 72C 30 sec DNA 30 ng 72C 7 mi Taq 0 6U LA Total 15 ud DNA 20 ng 1 TAKARA Ex Taq 9 DNA FF0 PF DNA F001F01 PF 2 9 2 B201 FAX 029 852 9351 E mail rinfo rizo co jp http www rizo co jp Copyright e2009 2011 RIZO Inc All Right Reserved
5. 6OO 1 1 1 1 v 6OO 15 OOO rom lt 20 25C 10 20O 1 15 ml 200 gl 15 OOO rom lt 20 25C 10 70 8OO wl 15 OOO rom 20 lt 25C 10 10 5O1 TE PCR 6 DNA DNA 1 5 m 1 25 50 mg 6OO1 1 e600g1 1 1 V Y 15 OOO rpm 10 20 25C 200 1
6. DNA S OOOZ CDNA Ver 1 0 RIZO Inc DNA DNA OO 0 NA 0 0 WW DNA DNA DNA DNA PCR DNA 85 mL 140 4C 1
7. 1 5 m 1 200 gl 15 OOO rpm 10 SOO wl 7O 15 000 rpm 10 DNA DNA DNA PCR TE PCR PCR 4
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