T8-FITC
Contents
1. 3 4 5 6 7 8 1 2 Q Prep EPICS iB FS Q Prep 0 5mL 3 FITC Log 1 CD8 1 Q Prep 2 CD82. 1 12mm x 75mm 2 100L 3 10 L MSlgG1 FITC 10L 4 10 12 5 TQ Prep Multi Q Prep 6 25 35 EICS 7 2 2 2 4 2 lt 8C 24 2
3. 4 90 5 6 WT PT 7 PAE
4. 1 29 29 T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3
5. 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC secon COULTER 6603861_0911
6. TQ Prep Multi Q Prep 11 NK CD8 NK CD8 CD8 T CD8 NK T8 FITC 2 CD3 T o 1 100 1 TQ Prep Multi Q Prep
7. 7546999 300 TQ Prep Multi Q Prep 3 D A Q B C 2 1 6603152 300 1mL PBS 24mL 9 259 6 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3 MslgG1 FITC T8 FITC 6603864 50 0 5mL
8. T T CD4 T CD8 2 T CD8 T8 T RLO CD8 T No
9. T4 T8 CD4 19 65 T8 EPICS PROFILE S PLUS IV T8 FITC n 9 Min Max Mean 1SD T8 19 33 28 4 T8 mm 236 1 102 595258 T8 CD8 CD8
10. 6 N 1 2 8 2 20 25 3 2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 T8 FITC 6603861 50 0 5mL T8 FITC McMichael AJ ed Leukocyte Typing IIl White Cell Differentiation Antigens 1987 Oxford Oxford University Press p 202 206 Morimoto C Letvin NL Distaso JA Aldrich WR and Schlossman SF 1985 The isolation and characterization of the human suppressor inducer T cell subset J Im
11. EDTA 1 100L 1 1 2mL 3 10 x 10 mm 10x 10 A mm 3x10 mm Q Prep PBS 20 25 6 a gt 10 x 10 mm 10 20 x10 2f 20 30 x10 3 30 40 x 10 5 40 60 x10 6 60 100 x10 10 100 200 x10 204 amp b 3 x 10 fil lmm
12. B CD20 85 10096 3 9 CV 5 1 0 19 3 4 5
13. 1 25C 500 xg 2 LH700 5 10x10 mm 1 100 uL 3 4 1 Q Prep TQ Prep Multi Q Prep Coulter Immunology 3 C A Q B CHEKI TQ Prep Multi Q Prep
14. 488nm 4 FS 9 SS 1 2 3 EDTA 6
15. 1 2 100L 10wL MSlgG1 FITC 10L 45 PBS 2 3mL JI z CH SEL 400 450 x g 5 PBS 25 1mL 30 2 7 250L nz HELET 9 PBS 2mL 10 400 450 x g 5 11 12 9 11 13 PBS 500 u L 14 EPICS
16. CD4 CD8 CD4 CD8 TA T8 T T T47T8 T4 T8 CD4 ARC AIDS related complex AIDS AIDS CD4 TA T8 0 CD8 T4 T8 ERU CD4 CD8 MS SLE T4 T8 CD4 CD8 f MS SLE
17. CD4 3 4 T4 T8 SLE CD8 f TAIT8 SLE CD4 CD8 SLE CD4 T4 T8 SLE SLE CD4 CD8 SLE T4 T8 TA T8 TA T8 CD4 CD8
18. Flow Count LH 700 mm mm x x 10 CYTO TROL 6604248 Fc Mo2 RD1 KC56 FITC CD14 CD45 Fc MSlgG1 FITC
19. fj 30 lt 359 CD8 CD8 class 1 MHC T8 SFCI21Thy2D3 CD8 BALB c J NS 1 AG4 lg IlgG1H kL l T8 FITC FITC Fluorescein isothiocyanate FITC 5 10 468 509nm 504 541nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN3 0 1 T 1 4 T CD8
20. 2009 11 2006 9 20200EZY00029000 6603861 877449 T T8 FITC 2 w A FITC Fluoresceinisothiocyanate 1 T8 FITC 0 03 0 15u g T8 CD8 76kD 32 34kD CD8 80 ERNO T
21. Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in active systemic lupus erythematosus J Rheumatol 16 1315 1319 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K 1987 Quantitative detect of CD4 2H4 cells in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells T4 4B4 and increase in T suppressor effector calls T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Ants B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 221 1 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 Reinherz EL Haynes BF Nadler LM and Bernsteln ID eds Leukocyte Typing II Human T Lymphocytes 1986 New York Springer Verlag p 8 9 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 1989 52 19 27 4 4
22. Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daley J and Schlossman SF 1987 Selective loss of the suppressor inducer T cell subset in progressive multiple sclerosis Analysis with anti 2H4 monoclonal antibody N Engl J Med 316 67 72 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets in patients with multiple sclerosis J Neuroimmunol 6 115 121 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus dellneation of subpopulations by clinical serologic and T cell marker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174
23. Wash No Wash Q Prep U TQ Prep Q Prep TQ Prep T8 CD8 T8 DIGeorge SCID B Epstein Barr CD8 AIDS HIV HIV CD4 CD4 CD4 T4 T8
24. munol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 Suppressor cells Eur J Immunol 16 198 204 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocytes Immunol Today 4 5 8 Reinherz EL Morimoto C Fitzgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cytotoxic T lymphocytes T4 and T8 effector T cells recognize Products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin Invest 68 699 705 Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 de Martini RM and Parker JW 1989 Immunologic alterations in Human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 Collier AC Meyers JD Corey L Murphy VL Roberts PL and Handsfield HH 1987 Cytomegalovirus infection in homosexual men Relationship to sexual practices antibody to human immunodeficiency virus and cell mediated immunity Am J Med 82 593 601
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