00ŒÚ - 機器分析施設
Contents
1.2. 8 00000000 a NN 100 00000 T 000000 hAASEROUT 5 0 4792 _ C4792 01 Erreak OUT gt 20000000 aN GP IB 00000 OOOO 000 0000 GPIB aL V 0 4 VIDEO OUT 7 00000 SI 0000 11 21 300 0 00000 00 000 00 0 00 00 00000 00000 00000 60 SCALED 0 0 0 TIME SCALED C4720 O 1 10ns 26200 O 00000 138 7 SCALED 0 000 8 ACQUISITION O MONITOR ON OO 0 IM 20000 00000 000 00 000 0 0 000 00 0 0000 0 00000
3. cotton effect 0000 141 0000 t 000 000 00 0000 0000 00 000 0 1800 101000 33000 33000 Ae 0 0 0 0 00 0 0 90000 0000000000 0 00 00000 00000000 0000000000600 ORDO abi ORD OR
4. 850 00 000 400 000000 00000 X 00000 gt 000000000000000 299 3 2 000 295 0 8 00000 Bragg O 000000 0 2000 000 DebyeSchere 0 200
5. 20 0 0 000 0 0 0000000000000 SI 00 2 1 0000 0 0 0 0 0 00 0 20
6. 000000 000000 00 000 0 0000 000000000 000 000000 4 P _ 8 lt 16 mm OOO 0 00000 00000000 00000 00000000 000 000 000000000 XYZ 26 gt 00000 298 0 00 0 00 00 25 0 0 0 000 00 00 0 000 0 0 16000000000 ENTERO 00O 0 000
7. 50 300 ml 1m 20 0 2 ml 50 2 ml 1 0 1 ml pH8 85 i 1 1 0 0 05 5ml 000 0 05 5 ml 2 mng 000 5ml 1 000 0 5 ml 60 0 5 ml OU 0 1N 5 m ul 324 8 nm 200 213 9 nm Fig2 54 Pa t ey Fig SIMAA 600000000 0000 00000000000000 00000 1990 55 00000 JES TE2000 000 000000000 4315 580 0000 0 0 5 Electron Spin Resonance 0000 5 9466920 00 3emM0 000000 X0000 0
8. Therrogavirety analysis scanning calorimary 5 Evolved gas analysis EGA 00000 0 0 5 0 580 00 0 0 000000000 DSCI 5 00000 05000 u 5 000000000
9. 0 0 000000 Fig 1 2 2 1000 900 000000 0000 00 0 000 00 0 0 1000 680 009090 0000 0 000000 1 0 000000000000 0000 950 0000 1200 0 5 3 1 1 0000000 000000000 0 PSO NORMALI I I JII I U ERROR 0000 VACUUM OOO 500000000
10. 136 II J 000 000000 cr 00 00000000000000 950 0 0000 NN 100 0000000 DL 50 00000000 4725 01 4729 000000000000 0O 4792 01 000 00000000 0000000000 INTO 0000000 50945 O C4334010 137 23 00000000000000 00 5760
11. 50 21 1 Gas Chromatography GC carrier gas 7 5 qe oag oag Oo Oo Oo MS detector column 00000000000 0000000000 000000 105Tor 0000000000 2 Mass Spectrometry 5 UUDJDUDUDDUDDGUUDUDDUUDDUUDUDJuGnUDDUUDUDUUDUDUupDnDUuunu UUDDUUDDUDDGUUDUDUUDDUUuDUDUGuGuUuBDuuuDpuunpBpunupuun 00000000000 000000000000 000000
12. 000 101 Onn Reflector m z ON Vref 30kV Onn Reflector m z Reduced Vref 27 5kV A Reflector m z Reduced Vref 13 5kV OO 5 2 OO N Myr Gly Ser Lys 1 3 Lys ____ eae pa eN lo 7 ae a 000 o r oo r N NN J P j 5000 4 4000 3000 1 12 2000 1000 la Joh 0 100 200 300 400 500 600 m z PSDO 00000 000 N Myr GSNK 00 0000 102 Collision induced dissocialion CID High nergy collision Higheegy colisog 0 000 50 Leg
13. 000000 00000000 OOUUUUU SI 000 19 O0 O XI U U H Rigaku RINT 2500 OOO 000000 0000000 4161 950 0000
14. transferasi O 0000000000000000 0 0 0 O 0 0 MALDI TOF 0 000 00000 105 60 0 gt pl 6 0 1st antibody Sheep anti c src polyclonal antibody 2nd antibody Rabbit anti Sheep SP HRP 106 0 kDa 80 0 kDa 60 0 kDa 49 5 kDa acidic 4 basic 6 0 Staining Coomassie brilliant blue G 250 000 Immunoblotting Analysis after Two Dimensional Electrophoresis O0 O Sheep anti c src polyclonal antibody 9 N Myr pp60 OD Coomassie brilliant blue G 2509 9 9 0 N Myr pp60 0 0 0 O 00000000 20 5 0 0000 000 000 0000 00 Shep ani csr polyclond antibody 0 0 0 western im
15. 109 5 000 0 0 000000 6 1950 00000000 00000 oc 0000000000000000000 NTA 000 0000 0 100 000 00000000 lU 250 C 000 300 CHOOO0O000000 100 200 300 400 500 00 M E Brown Intro
16. 00000 00000 500000000000 9990 00000 0 0 000 00001 0 000 000 AA ESRO 0000000000 00 0000 0000000000 6 2 034 9 1 9810 9200 9400 21 869 0 01 0000 5 000 ESR 7 7152 g 1 981 hv g BH B H AH _ j _ hv ay g 2 9810 O0 O0 vO 9200 9400 0 00 00 9 0 0000 00000 _ 655 1 335 7 0 90 0 SI 0000
17. 0 000 80 2 1 ESI 0000 5 Fg 9000 Rayleigh 88
18. 000000000 590 950 0000000800
19. 0 0 00 Ry Ro Y s Ho N CH CO NH CH CO 0000 PITC pers 5 Ro Na asco co PTC 0 OU NHC a i 2 R HN CH CO AZO H 0000 00 2 PTH 0 lU 2 2 00000 kymi 00000 OND 0000000000 R1 R2 R3 R4 528 548 400 180 400 200 100 1
20. 0 0 0000 124 0 0 0 0 0 0000000000 D D 0000 0000000 0000000000000 000 m B m CLB D EE Lee 16 37 Bie aay a B 4 Z 2 YF 4 24 yF BMICHSAT YF ONO KI AA VF 0000000000000 0 0 E O o o lV Vi 0000000 000 000 tuy ishu i 2 Oo Te EN o a EP 125 0 0 0 00000000000000 LSM GB2000 000000000
21. 000000 000 N 0 000 0 00 0 000 0 00 000 0000 00 0 0000 00 0 EVACOO O00 OFFOO 000000 0 RESETO 0 950 000 Fig 49 49 2 3 0 00000000 heho 0 00 22 5 0 148 Gep cyD OO 000 00000000000 00000 15 80 Fig 3 B 0 0 hGH G2 B CyD 0 0011000 1 2 00000000 0 0000000 00 00 0000 0000 0000 G2 B CyD 0 uuunuunn 92 14 100 50 9 1200 1400 1600 1800 18 G 1 7 92 gt i i B E 100 15 G gt 1 4 G 2 14 4 E 1200 1400 1600 1800 m z Fig 3 80 0000
22. 2 Laser 337 5 5 Flight tube Deflector iil High vacuum 4 pado P1P2 lon Lens 0000 00 SI 00 mM 000000000 VI 0 00 mUv 2 zeV 000 v 2zeV mU 000 000000000000 mo L xX1 xX200 000000 t L v 000 m z 2eV t L U m z Kt 000 2 012 1 929 x 10 V L e 1 602 x 107 0 U 0 0 0 0 0 atomic mass unit 0 U 1 6605 x 107 410 0000 0 000 000 m at b 000 ab Acceleration Voltage Length 2 Flight Time 12
23. J 00000000 O 950 5 52 12000000000 000 0 Sz V20000000000000000000 Ive 2684 3720 00 000 000 9 H 92741 10 90 900000000 0000 2 002310 0000 56 f gBHI2 1 0 Cee ee Ne x a S 1 2 21 00000 00 0000 OO S420 1 321 2 10 0000000000000 o SH 5 1 2010 950 00000
24. SI 000 000 0000 00 000 0000 00 0000 00 mood 000 1998 00 000 00 000 Moog 1997 00 001 1981 78 1 0 0 000000 00000 22 100000000 00000000000 PEG DEA 21 00 00000000000 a 00000 2000000000000000 NPOE 3000000000000 NBA P
25. J F 00000000 5 20 0 000 00000 580 00 Micoa 2 00 000 0000 00 00000 00 00000 00 0 0 0000 00000000 12 00000000 X Y OOOO 00 00000000 0000000 Ba EST uu uunnuunnuunn O00 Microcal MC 2 J I 00000 114 2 5 0 D SI 00 00000000000 MC2 0000000000 10 00000 10 20 30 45 60 12mg 0000000 0 0 0 35 0000000000
26. 122 SI 00000000
27. 72 9590 0 0 0 1 005 40 3701 00 0000 10 0 4 0 00000 32 50x504 mill J JU 10 0 0 000000000 27000 0000000 0 0 910 00 0000000 3200 000000 00000000000000 000 270 722 0 37220 3757671 20 0 D 0 ABSORBANCE WAVENUMBERS cm 1 00000000000 Figg 00000000 000000000 OOUUUU CM1 ABSORBANCE 0 000 4000 3800 3600 3400 3200 3000 2800 WAVENUMBERSI cm I Fg 2 000000000000000 73 4 OO00 0 DX 303HF BU 20GC mate 00000000 482
28. 1 1 000 ul O pa 000000000000 00000000000000 52 00000 WV J l SI 00000
29. 0 30 0 000000 0 2 2 0 0000 0 00 0000 0 0 0 0000 OOOONMR 0 0 0000000000 270 1 400 MHz 500 00 000000 0000 UID 2 0MHz 0000 0000 ON 0000 0000 00000 00000 0000 0 RENE 1 000 13C ME COM DEPT 135 3 4 ME DEPT RDM
30. 0 2 00 4 21 3 4 H 61 31 0 0 00000 Y 0000 CRTOOO KBr ggg 00000 D000000000000000000 D000000000000000000 D000000000000000000 Q 0000000000000000 0 0 00 00000000000 D00000000000000000000 O KBr 70 000000 000
31. 000000 510001 1 O 3009920 000000 00000060 0 50 OOOOOOOOOOOOOOOOU 8 000000000 1
32. 2001 3 5 000000 000000000 00 950 0000 10000 19891 21000 1988 3000 5 000000000000 00 0 880 0000000 1980 87 HITACHI M 12000 1 5 0000000000 0000000 4168 80 0000 5 5 OOOOOLCO Electrospray lonization Atmospheric Chemical lonization O 0 ESIQ
33. N Lo lt lt 00 000000000 16 10 00000 00000000 490000 159 0 0 0 0000000000 0 0 0 000000 0000 OOO HO HI
34. 40 0 00 0 00000 0 0 0 0000000 0000000000000 000 000000 000000000000 00000 00 5 0000000000000 000000 2000 000 000000000 00 0000 00000 0000 IBM DOSO 0 0 00 720 1 44 6000000 RINT 200 6 0000 000 0 00000 20600 000 10 000000000000000 0 OFF OFFOUO Powe ON 0000 00 9 0 0 0 Powe 000000 Logging OO shutdown 000000 00 0000000 Halted you may now 000000 OFF 00000 5 OFF 00000 2 000000000 OFF SI 2500 18
35. 872 0854 000000000000 50 00000600060 2 1 00000000 N 2 _ 0000 9000 112 Pa 000000000 a 2 00000 000000 102 00000 00 37 0 0 0 0 000 0 00000000 0 0 00000
36. 4 82 x 10528 1 0 entrance lens ion focus cylinder drawout cylinder drawout plate m filament 00000 ion focus plate I 000000000 repeller 000000000 lens insulator inlet filament 00000 0000000 91 X ray lens m erress filter 0000000000000 2 2 010 00000 1 0 00 00 0 0 2 00000000000 0 GC MSD ALS 00 0 0 0 0 000 1 WINO DD D D D D Windows 000000 6 4 00000 000 000 00 00 0 w 6 100 000 2 000 0000 000 0 000 0 0 000 0 0 00 0 00000 3 000000 4
37. 800 14 SI 000000 0000000000 0 00000 so 00 000 9 0 00000000 9 Svedbergunit s GOO0O0000000 In max R min 10 _ 2 53 X10 R min 3600 rpm 1000 15 ks 00000020 0000 Ty 70 1Ti 00000 40 000 00000000 00 00 O0000000000 0000 36000 php 000000000 00000000 0 0 70 1 Roe 20 5000000 _ 2 53 X10 X In 82 0 405 40 000 1000 111 5 223 05 111 5 Type45ri 00 0 36 000 prd 00000000060 _ 2 53 X10 X In 103 8 35 9 38 000 1000 5 2
38. Hg 0 0 U sx 100 QHO SH 199 Sc 000 J U 0 Fnx 56 200 SN W OH 0000000 p gd P U 0000000 mg 5 SoS Q UHUDUDUUDUDBDUUDUDUDUHugcort XN 00000000000 county OOO FN I O00 HO 0000 17 5 l 0 L K K K K S EO 0000 00000 000000000 100000000 20 25 0 00000000 0000000000000 180 200 0 0 YES 0000 0000 0000000000 OWAITINGG 00 0000 M1100 OPERATION START YES OR 5 000000 150 20
39. Commands 00000 Multi 2 00000000000000 Ce 100000000 0 0 0000 000 Shiftl 00000 0000000 49
40. 580 00 SI
41. 610 000000 0 000000000600 000 150 80000 00 0000 0 00000 00 0 0 2 RRO CRT OAR AHAA REM it tvVR om on 1 SF GAIN 2 am s 3 PM VOLT 4 OFFSET 0 STOPPED FLOW 103 D i 3 Y 41 on ma 12 13 15 16 17 18 19 20 splay Scale t d e t 0 2 ABU Scale Petit SF SF O1A 10 B gt CH C 117 CH B gt 12 CH A CH B gt CH C 13 CH A lt gt CH C 14 CH A average gt 15 Smoothing Differential 0 300 asec disk s e disk 21 22 23 24 25 28 21 28 28 30 31 32 33 34 35 36 37 38 39 40 RETURN 1 1 17 Log plot 18 Guggenheim plot 19 Curve fit RETURN 41 61 42 62 43 63 44 64 45 65 46 66 41 67 48 88 49 63 50 10 5 11 52 12 53 73 54 14 55 15 56 18 67 11 58 18 69 78 60 80 or 80 151 91 0 0 0 0 0 0 0 0 0 0 00 0 0 00000 Trig 0 000000 00 0000 10 0 0 STARTO
42. 1 52808 00 0000000000000 00 JUD JUD 2 0000 OND O0 O 0 0 0 STARTO 0 0 0 5 5 IU U 0 000 1 2 0 000000 000
43. ol SS o o a a ee 0000 Instruction LSM GB2000 Olympus 0010600 HHB DD oa a o o o oE O o o o a O ELE E o o o o o a a a o oE o E o a a 129 O FUJ IX 5 1000 000 00000000 00 0000 4357 000000 4359 580 0000 00000 0 000 0 00 000 0000 5 BASH 1987 00 0000000000 0 0000000 000 1 060000 0002 OOOO003 000000000 00000000000000000000
44. 0 0 0 0 0 0 00 M gt mit mt mt 000000000 4 M gt mitt m3 O p Ps 0 N2 Laser 337 575 Deflector Detector co G i FastFilter i Pl P2 0000 Iontes 4 Detector 0 0 0 0 0000 00 000 00000 103 ai 200 MOM mz 00 9 M 8 fen lle Met AEAMSQVTNTATIM Omin L i oh call A j a T T T T T 1200 1300 1400 1500 1600 mz HIV 1 p2 pepti
45. 5 ON POWER OFF 1201 0000000000000000 OFF 00000000 SI 0000000000 5500 00000 000000 55100 0000 25 000 7 70 J 04 0000 200 0 150 000 60rmp 400 0 150 000 WOOd 1000 0 150 000 0000000 0000000 20 0000 000000 I B 3 5 mM 950 000 5 2 3000000 120 2 00000000600 3 00000 121 000000 15 682000 000 0000000 0000000 4334 90 0009000000000
46. 10 00 0 pH430 0 00 00500 0 00 4000 2007 0000 2251 0 0000 00000 00 MEAE Pn 2 00000000000000000 0 2200 300nm 00000 00 0 000 UU O000000000 0000 COO 00 211 00000 9 1 0 0000 000 DNAD 0 4 0 0 00000 00 00 00 OO00 00 DNAD L dc D dC 9 00 LD cC 00000 00 0 00 0 00 0 0 0 000 000000000000 00 NAQ 0 0 U OOOO OD t dc 0 9 00 00000060 OOO000000 0 D dac 0 000 146 OCD 4 GRLeEFUAIV MOCDAN FL a L dC 1 40 12 1 bXL dA 2 L dT 12 BLO D dA 12 4 12 00000 4010 000 00000
47. 0 0 0 00 0000 75 HO Cl chevicalioniztio 000000 Els TA Estes fal 000 0 OO fast tomborrbardrert 00000 000000000 00000000000 M y0 000
48. 0 0500 000 100 0000000 5 0 0 BO FomBQ D D Form B a no Form subB d d ee oe e 2 5 Form subB 0 FomBO OOO 20 0 25 50 75 100 20 0 25 50 75 100 0 0 0 1 C min b 10 C min 20 d 40 C min 80 C min 112 10 OO00000000 DSC 00000 1 00000000 67 00000 000000 88 67 00000000000 0 00 10 55 00 0000000 000 0000000000600 60 0 0 88 5 FomsubB 0 00 00000000 55 FomsubOOOO0000 00000000000000 200 40 5408 00000 0 25 Fomsubb j Form D 00000 FomDI I H I 00 FormsibB 000 5 00000 0000 00000 0000 000000 CP
49. 0 000 lt 000 YY i lt gt gt lt lt gt iS popon 000 00000 D lt 000 OO 67 00000000000 000 580 0000 000000 0 Attenuated Tota Reflection ATRO 0000000 0000000000000 0 Scanning Cdorimeef DSC KBro 0000
50. 000000 I FLOWI 0 0 0 0 AUTOD 0 0 0 0 MANUO OO 000000000000 0000000 0 0 IPRESSII 0000 ON 000 0 00 000 000 IFLOWIJ OOOO 00000 0 00000 0 00 0 0 00 0000 4290 00 20 0000000000 430 FLOWN 0 0 0 0 AUTON O000000000000 S PRESSI 0 0 0 0 OFFO FLOW 0 0 0 U MANUG O l OFFO 480 00000 00 POWER 0 0 0 OFFOOO 00000 00000 00 0000 0 log 00 00 0000 0 0 STOPPED FLOW SF SF 01A Display CH A B gt CH C CH A CH B gt CH C CH A CH B gt CH C Y Scale gt lt gt CH C 0 2 ABU CH A average gt CH C Time Scale gt Smoothing Differential 0 300 asec plot Guggenheim plot fit RETURN M e n u Select 0 RETURN 153 21100 000 0000 00000 0000 000 M
51. LO OU 00000 LU OO 600 000 SHIM FILE SM4 43 51
52. DOI I I I J 5 1P 2P 5P 5 2 QO 00000 0 SPINOFF LORDAJECT 0 000 off 000 2700 00 0 0 400 A 5000 00000 0000 00 0 0000 000 0 0 00000 SG 270 00000000 Hu 009 00 00 000 0 0 GX 400 00000000 Hu n 500 00 0 0 000 00 00000 000 DEC 3000 270 GX 400 500 0 00 000 000 0000 00 00 0 0 000 42 Fach OOOO 3000 0000000000 SI 000000 H OOU
53. 000000000000 20 0 57 BHBOR SWE Pet past m as sss HEHEA a 0 0 0 uu 0000000000 000 0000000000000 000000 poel J J 58 0 5 0 1 0 0 0 00000 00000000000 0 0 PARAMETER SETS 000000000
54. 30 gt mkdir cytidine 0000 000000000000 ftp rasa gt cd cytidine 000000000 ftp rasa gt pwd CR 000000000 ftp rasa gt put afc7R dat CR file default afc7r dat ftp rasa gt put afc7r psi foreign file default afc7r psi ftp rasa gt put texray inf CR foreign file default texray inf ftp rasa gt bye 000000 3500000000000000000 0000000000 0 000 C X CYTIDINE gt COPY 000000000000000 000000000000000000000 C XYCYTIDINE gt DEL CR 0000000000 C X CYTIDINE gt CD 0000000000 C X CYTIDINE gt RD CYTIDINE CR 010000 0 CYTIDINEG QQ C X gt SHUTDOWN 850 0000 SGI indigo wsp 00000000000 0000000000000 0 tesa 000000000000000 Password XXXX 0000000000 000 1099500 00000000 00000000000 9 0000000 0 CR 2000000000000 15 000000000000000000000000000000
55. 0 0 0 0 21 0 0 O 0 AUTOD PRESSO I 000 149 44O000000000 50 PRESS O OO 00 ONO 6100000000000 DURATION O 0000 0 00 0000 10000 00000 DU 910000000000 2 0 0 00 11000000000 10 00 How many files 0 15 1 Oo 0 0000000000000000 lt gt STOPPED FLOW sxxex SF 01 Test 10 Samp period 300 Measure 1 SF Ga ln 2 ABU auto accus Accum 0 13 Wavelength 550 Slit clear 14 1 4 na 19 Save condition gt Calculation CE Select 0 21 0000000000 Tesh 000 0 0000 000 00 0000 0 000 0000 0000 00 0 1 41mi 0 00 0 000 0 0 1 50 000000 44 O00 00 OO 51 CRTO OOOO fPM VOLTO 0 0 0
56. 5 O00000 117 000 5 5100 D 00000000 0000 4808 580 0000 1932 Bruche Ruska 5510 SI 00 2 1 0000000 0 Scanning dectron Microscope Semp 00 0000 00 00 UI Fig 1 Fig 1 5
57. 0 0 0 71 UO 3751 0 0 0 0 S l OOUUUUU BUUUU MUUUUUUU 0000 10000 000 MUUUUUUUY MUUUUUUU 0000 000 BUUUL BMUUUUUUUU 0 0 01 5 40000 0000000000004000 1 6 0000000 0 0 0 2000 00000000 0 010 00 2 4 0000 400 10 000 2 000 0 Gel KBr 5 690 9 DLATGSI Csi 50000000000000 32 bit CPU 810000 1100000 1645 00 00000 0000 00000 00000
58. 4 O0000000000000000000000000000005 0000 950 0000000800 0 0000 510000 130 510001 5 OOOO00 0000000
59. SI 0 00000000000000 00000 0000 rpm g 457 45 000 235 000 133 6 x 96 0 nL 70 1Ti 70 000 450 000 36 12 x 13 5 mL SW 28 28 000 141 000 246 6 x 39 0 nL TIDI 000 528 100 10000 OO 7 65 65 000 0 0 0 0000000000 0000600 PEEB ED
60. H 000000000 00000 000 0 0 0 00000 HOUUUUUUUUUUUUUU 01 127 H n 0 0 0 0000 Mos Image only with 128 Mos
61. 0 0 0 0000 0 0 000 510001 O O0 I I J J 0 50 O 0 UNIXO aw a 1000000000000000 00000000 000000 0 131 10 000000 00000 00000000000 00 bslgo00000000000cOO 000000000000000 0 READY 56 000000000000 0000000000 1 00 0 5 I 0000000 T IP Scmner II 000 0 000 00 0 0000 0 000 000 lregeFileNare Typein 0 0000000000000
62. 1 0 4210 297 mm 00000 50 0000000 0000000 400 0000 0000 000000000 100000000 000000000 0 Poso 0 270 2 INTI FREQ POSITION BAR GRAPHO 00000000000000 0000000 0000000 Commands O O00 0 00000 95 SOO00000000000000000 00000 0 zv OO FileQ 0000 Print 00000 47 PrintSdectionG 00000 00 00000 0 0 00000 0 0 0 00 0000 Print 000 00000 00 0000
63. Commands 00 0 0 0000000000000 0000000 0000 Reffeencest 00000000000000000 5 0000000000000 mO0000000000000000000 0000000000 00000000 Commds 00000 46 0000000 00000 THO File 0 0 Printer Setup 4210 297 00000 00000 00000 00000 0 00000 0000 00000 3
64. 540 MENU 100000000 MENU MENU SAMPL Comrend auto 5 00000000 Accumulation as 5 000 00000 0 0000000000000000 9 015000 10 000000000000000000000000000000 00000 UPDIS 1 0000000000000 9 615000 00000000000000 5 0 00000 00FD0000FD0000000000000000000 saep000000000000000000000000 000000000000000000000000 es 00000000 miso ooo 000 00000 000000000000000 Exponniag BFO 01200000000000 0000000000000000 4000000000 0000000 wej000000000000000 Pog P1O000000000000000000 1 2 P0000000 000000000
65. 85 R Narasaki T M Otagin Biochim Biophys Acta 1338 275 281 1997 140 00000 7200 000 000000 4153 80 0000 1817 liri 0000 0000 00000 0 00 0 0 000 0 000 0 0 0 00000000000000 opica rortetory dispersion ORD 0 00 OOO 00 19550 H Rudolphi 000000 0O 00O 0 170 1000 000 O00 Grcula dichroism 1 0 SI uu
66. 2 0000000000 22 0 1100 0000000000000 0 0 0 0 00 0 0 0 0 0 0 0 0 14 5 0 0 O 0 MAC GAING l GO00000000 0 191 000 00 0000 00 6 0000000000 16 ACQUISITIONG 0 0 0 MCP ONO OOO 2 00000000000 00 0000 000 0 0 00 0 0 0 0 0 0 0 000 OFFO OOO MCPGAING 0 0000000 00000000000 0 1000 00000 0 00000 180 000 00 0 0 00 0 000 0 0 0 0 0 0 0 Photon Counting 0001 p O0 0000 000 0 U 1910 00000000 0 0 SCALEQ WAVELENGTHI I 0 00 200000000000000000 000 0000 0 0 O 00000 20100000000 MONITOR 0000000 000 1 3 00000 Photon counting 0000000 22 LASERI 0000000000 0 0 0 00 0 0
67. 600 000 010 000000 00000000 SI 0000000000 900 GC HP5890 000 1 5 5972 0 0 0 0 2000000000 000 0000 0000000000000 0000 1 60 700 0114 0 000 HOU 0000000 1000991 00000000 U S N gt 40 86 00000000 1800 0000 3001000 500 000 000000000 65 10 90000 0000 HP486 66 0000000 10000 950 0900900000 371 4153 000000000 950 000 0000
68. 107 GSNK peptide 7 coNAD O 0000 0000000000600 ell L 0 O Peptide W Fattyacylase lJ Peptide 0000 N Myr GNAAAARR M H 1047 20 997 58 uy 1000 SS TTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTITITTTTTTITTTTTTTTTITTTTTTTTTTTTI 0 100 200 300 400 500 600 700 900 1 000 2 2000 GNAAAARR J M H 1000 4 786 3 i 20 3 TT 1 0 100 200 300 400 500 600 700 800 1000 m z HOU Peptide N Fattyacylasd 0 N Myr GNAAAARR peptide O 000000000000 108 O PERKIN ELMER 5 7 OOO 000000 0000000 4161 950 0000
69. 110 00000000000 20 400 00 0000 00000 21 PRESSO I O O O OFFO O 0 0 0 AUTO gt gt MANU gt gt AUTOD OOO PRESSO OOOO 000 00000 00 00 81 91 STARTO 1000 00000000000 1 PRESS O OO 00 OO 21 IFLOWII OOOO 0 00000 00 0 0000 00 0 00 0000 1 55 0 0000 OFFO 30 0000 0 00000 OO00C00C0000000000 00 0 0 0 0 0 0 MANU gt AUTOD OOO OOOO PRESSO 0 510000000000 caoot ROCO 0 O OND OOOTFLOWQ 00 0 000 810 000000000
70. OOUUUU OU OU ina in 209 lt y V E at 76 950 00000 00000 JMS DX303HF 000000000 JMS DA5000 000000 00000000 25 0001096 UD 0 05ng 0 N gt 10 0000 mz 1 6 900 0 0 00 1kV 10 2 3000 0 0 0 0 1 500 800 501 20000 000000000 EI FAB 00000 5 20 000000000 0050 V PC Windows3 1 000000 000000000 000 5 000 20 1 0 03ng 50 N 10 0000 mz 1 10000 00 0 0 2 5kV El CI s0 UUDUDUDUUDUDUDUBUBDDUDDUDBDDUDUDUGCres 0 0 20000 000 00000 GCnete O O ODO O El 0 000000 00 000 000 0000 0 0 samples in the file list have been conpleted Clear thelit 0 0000000 0010000000
71. Ultra Clear mm 12 2 1 2 00000000000 0000 00 000 000 00 5100000 OUUU 6 100
72. 20 20 2026 000 00000000000 000 SI 1000 21000 0 000 2900 00 0 X ray 0 0 0 000000 TEM LW OlIGQO0000 0 200 2 X00000 0000 00000 00000000 0 16900 ON 0 ON SEIKO 1 ON 0 SEIKOD scan 1 0000000000 0 Login OO 0000 00 Password 00 0000 RINT2500xG0 00 000 Powe ON 00000000 00000 ON 0 0000000000 3 0000 000 0 0000 000000 0 000 0 00000 0 00000 00 000000 0 000000000000000 0000 0000 21 0000000000 0 00
73. 790 830 000 0000 000 000 1 8 00000000 0 820 8 00 0 00000 000000000 Meard 1 sae f 2 cursor I l I J U J U 1 f 3 f 6 2 0 PMT Vo OOO0O0000 7 pek 10000000 18 po 00000 143 0 180 0000000 0000006060 0000000 2 Matheretics 0 0 20 0000000000000
74. MALOO 950 000 MALDI TOFMSHO OOOO Ddayed Extraction 0 00 000000000 Dda Exration 000 0000000 0000000600 5 5 5 0 0 Post source decay PSD Low energy collision Postsource decay PSD 0 000 0 00000
75. 0000 6 8000 200000000 30 6000000000000 000000 6800000000070 gt I 20 S D 2000000000 0 2 44 6 0000000000000003 00 000000000000000000 00000 000000 H6 8 u 0 2 50 1 mol 680 yy A 00 0000000000 62 8 00 8 0 D 5 050 000 4000 72000 000 5 8 0 000000 62000 5 50 00000 0 4060 80 100 000 q 0 0 050 0 0000000000000 A S B CyD 000 C SBE7 B CyD D SBE4 B CyD G2 B CyD 950 000 0000
76. 3 000 0 10 INTERMEDIATE APERTURE 0 2 0 4 Aperture 10 0 0 INTERMEDIATE 0 J 00 0 020000000000 00000000 O Aperture 10 O INTERMEDIATE 000000000 5 Aperture200000000MSoo000000000 0 00000000000000000000 u 0000 01 6 DO0O0 00000 Aperture 19 000 INTERMEDIATE APERTURE 0 0 0 7 EVACO OOOO 8 0 2000 0N 00000000000 SI 0000000000 M 1200HQ UH 0000 HITACHI L 6200 000000000000 5 000 HITACHI 1200 LC MS 0 0 0 0000 m z 10 2000 0 m z 10 800 0 3 amu m z 801 1200 0 4 amu 01 2 0000 Hood HEWLETT PACKARD 486 33U OS IBM OS 2 Version 2 0 Hood HEWLETT PACKARD OD OO000 000000 0 0 00 2 0000000000000 SI 00000000 000 5000000000000000000000000 GOO00 000000000000 0000
77. 1 22 42 62 seuss STOPPED FLOF SF 01 i 4 5 26 45 85 8 26 48 1 27 67 24 88 8 29 48 10 30 50 10 3 81 12 32 52 12 13 53 13 1 64 14 16 36 55 75 18 38 58 18 RETURN n u 3 57 18 38 58 18 7 ic it 30 00000 00 000 00 0 000 00000000 000 fromto 0 0000000 0000000 PERIODI 11 SF GAIN 1415079 000 00001900000 4 RETURN Next 0 Plot 1 2 Digtd 000000 0 10000 1210 00 0 00 Comet 00000 CO Zo thw 2 STOPPED FLOP 5 1993 03 20 16 2 Dete 83 03 20 Tine 14 49 47 ___ 20 ssec 10 5111 1 4 0 1783 80 6 07976 160 as 0 05626 240 as 0 04988 10 ms 0 1635 80 0 07379 170 0 05450 250 ss 0 04811 20 0 1421 xs 0 06873 180 0 05317 260 0 04839 30 ws 0 1252 110 as 0 06655 190 us 0 05188 270 us 0 04876 40 0 1122 120 0 06286 200 0 06118 280 0 04774 0 1008 30 0 08078 210 as 0 06149 280 as 0 04782 140 0 0587
78. SI 000000 BHS 2 0 17 mm 0 17 mm
79. 0000000 Ost 000000000 0 00000 0000 00000000000000 Recall fill O OO O00 0 Recall 000 0 DSC daad 000 0000 Swap curves OO 0 0 5 0000 111 00000000 0 0O O ShutdowntheUNIX operating syster 000 0 cPu ooog OFF OFF 0 05 70 0 0 0000 0 200 0 05 70 0 0 OOO OFF III 00000 OFF SI 000000 0000000 000000 000000 2 00 20000 0 00000 850 000 0000000 0 FormA Forms 5068
80. 24 PS Printer SGI WS rasa2 SGI WS MOLGRAPH Room 104 gt KUIC DEC PG RASA 7R 0000000000 xsi 0 read 00 000 5 Dakin rasa 0 0 000 SGI Indigo2I 0000000000000 0 0000000000000 00 00 00000 SGI WS R No 1 CD ROM lt Room No 105 DAT PS Printer teXsan S B Rigaku RASA R 0 0000000000000000 UWUUUUUUUUUO OOUUUU 18kw 0000 2kv 60kV 1kV step 0000 10nA 0 300 1 step 00000 Mo 0000000 000000 15 C 25 0 000000000 DEC PC Rigaku AFC Control Softweare CTR 00000000 SGI Indigo teXsan 000000000 0 SGI Indigo2 Dakin MOL MOLIS MOL CRY S 80 0000 0 0 2 0 2 0 2 0 0000000000000000 0 0000000000000 0 0 000000 0000000000 000000 0000000000000 25 Bong 00
81. 00000000 00000000 Type 451 Polycarbonate Bottle Assembly Cat 355622 Type 70 1 Ti Polycarbonate Bottle Assembly Cat 355603 SW 28 Polyallomer Cat 326823 0 0 O Ultra Clear Cat 344058 11 000 000 tend 0 00 00 0 00000000 950 0000 LIU UU UU UU 0 201000800 VACUUM START STOP 000 000 000 POWER 000 0000 00000 HOLD VACUUM UU UU UU 000000 0 0 0 0 0 OOO POWERO OOOO 000 H 2 000001000000 0 0 HOLDO 0 0 0 l HOLD 00 0000 000 0000 00 0 00 0000 800 800rpp 1 1
82. 10 00 00 00 00 00 000 0 0000 83 1 u 000000 1997 2 0 150000000000 0000000000 00 35 36 1997 93 Bruker REFLEX MALDI TOF 5 000 000000 4367 80 0000 0000 180 0000 0 0000 00 0000 0 0 000 00 00000 0 000000000 fast aomborbardment 000000000000 0000 00 naix assistedlaser desorption ionization MALDIV O 00000 Bruker REFLEX Marix Assisted Laser Desorption Time of Flight Mass Spectrorreter MALDI TOF MS S Ha HBH D MALDI TOF MS
83. 11 00000000000000 2000000 00000000000 2 1 0000000000000000 0000000000 Real Time Plot Parameter TimeWindow 0 00000000000000 lon Plot Type Plot7Z1 2 0 O Tota 000000000 8 0 000 9 00000000000000 SdectIniet 00000 A B ConstantFlow HOOOOOO0O00000 O0000 Inlet A B Pressure Program Init Pres 0 0 Initia Time DO OOOO 2 GC Pressure Units ODO O00 pig EntredValus 00 000 0 00 000 0 0 0 0 000 0 00 0 0 0 00 000 84 CaculaedValues I Split 00 0000 000 0000 00000 0 0000 0 10 Aux Pressure 0000000000 11 601 000 D Zone 0 0 0 000000000 Oven Parameter 0 0 0000 Oven 0000000000000 000000000000 Cryo On Cryo amp amp 0 000000000000 Oven Program Init 1000 Init 0000000000 Led12 3 00000000 320 000 0 000 0 000 00 0 00000 0 12 00000 000 Source M anual 0 0000 000 Location 4
84. 0 100 00000 000600 KBro OG KBr es lld 0 0 0000000 0 OO oe o0000000000000000 ggg 75009 DDD 0000 000000 00000000 2 5 1 0 0 000000000 0 4
85. 0 000 etj ni nun Mod Meu eth 00000000000000 0000 017 OOOO000 MSC Software teXsan UNIX TOO 0 0000 00000 Sysemi 00 000 0 1 32 0 MSC SoftwareteXsan 30000 H 000000000000 MOLGRAPH MOL MOLIS MOL CRYS 0000000 0 00 MOLGRAPHII 00000 000 00 0000 00
86. 00 0000000000000 000000 LAUE LIMIT PRECELL COLLECT 0 000000000000 CELL 00000000000 0 PSI YOOOO000000000 0000000000 0 C X CY TIDINE gt dinj AFCIR XTL AFC7R LOG 00000000000000000000000 COLLECT OUT 0000000000000000000000 AFC7R DAT AFC7R PSI 000000000 0000000000 TEXRAY INFOOOO 0000 00000 00000 00 000 C XYCYTIDINE gt PROCMENU CRU J 00 0 000 00 0 000 0 00 0 000 Cell Contents Approx of non H atoms in cell 58 vol 20 0 52 Element symbol Number in formula Atomic number 9 00 13 00 5 00 3 00 The number of atom types id 4 Is the formila correct Y Estimated Z value based vol 18 0 3 4 vol 20 00 3 1 O i Number of formula units 0 0 29 15 coret 0000000000 lt gt 0000000
87. lt gt 0000000000 0000 lt TRL z gt 0 0000 lt RETURN gt D 00000 000000 vol 18 3 4 vol 29 31 000000000000000 00 000 400000 lt gt 00000 Space Group Selection Enter space group number from International tables Vol A or I Space group number Available settings 1 2 e 4 Setting choice 1 Number of asymmetric units 0 Observed density 0 000 Intemational Tables for X ray Crystallography TO OOO 000 000 000 0 00 0 00 0 00000 OOOO 00000000 000 000000 000 0 lt TRL z gt 0 0 0 0 Structural Solution library MITHRIL general direct methods NONE direct methods not run after processing Principal authors C J Gilmore University of Glasgow MITHRILO lt TRL z gt 0 000 00 5 000000 C X CYTIDINE gt ftp rasa CR Userid for logging in texsan CR Password for logging in as texan on rasa CR 00000000000000000 User texan loggedin 00 00 00 Login incorrect PASS command not understand O 000 00 bel OO 000000000 00000000 gt 15 CR
88. 23m0 00000000 50 000 FG OOOOO 0000 OOOO 20 0 0 00000 J NM AL 300 000 4381 000 000000000 0000000 4815 80 0000 H NMR 0 0000000 0000000 270MHzNMRO OOOO 0 SAMPL Commend O sr 0000000
89. 155 00000 156
90. 20 9 23 Rigaku AFC 6 Rigaku 7 000 000000000 00 0000 4579 000 000000000 4310 950 0000 Hauptmann 000000 1001 1040 105000 00000000000 RigakuAFC Rigaku 5 7 DEC SGI Indigo
91. 000006060 186 0 0 834 hr 50 min 15 OUUU 0000 MT 3 MT 5 00000000 000000 4829 950 0000 000 950 00 CO 00000 HO OOU H 04CO HHe H a
92. 4 00 0 00 01 0200 00 0000000 Visuvg o 10 0000 00 50 0000 0 RA 4010 000000000000 000000 45480 185 ANALOG DIGITAL 400 0000 SI 0000 00 00 0 0 000000000000 000 00000 00000000 0000 10m 0 000000000000000 1000000 5 0 0 0000 ongo o 3000 RA 401S 0 000000000 OPERATION OFFO FLOW PRESS OFF J PM VOLT 6 00 0000 14 7 2000 350nm VIS 3500 800nm 148
93. 45 7011 600000000000 0000 280000000 950 00000 E gt 0 oooo 0000000060 DDD 0000000000000000 0 90000012 i 7 10 10
94. 00000 E Mail ORTEP kV mV mPa mV mPa PC WS usr people texsan 36 PL PB PI 270 400 A 500 OOO 000000 000000 4381 4815 SI 0000 1945 I 00000 Bloch 00000 0 0000 0 0 00000 219531 060 0000000 0 00 00 00000 19700 FT NMRO 9000 00000000 000
95. 1 ESRO 27 20 0 000 0 00000 0000 00000 JIR 6500W 00000000 4809 80 0000 molecula vibton 0000 Infrared Seetu ROO 0000 00 0 00 0000 000 250 25u m l g 40000 400 Fourie Trensforminfrered 5 FT IROO 0 00 SI 00
96. PO 32768 1 4 9 450 00 1H PW1 4 9usec 1H0 450 13C PW1 4 lusec 13C0 450pulse DEPT PW1 8 2usec PW2 20usec PW3 30usec 2 10000 SL CDCL3 0000000 020 C5D5N ACETN DMSO DS DF 000000000000 AUTOD SwitcH 0000 sample load spin AUTO LOCK ON SHIM ON 00 0 TCON 0 ON 0 0000 0000 0000000600 5 Tl XXXX 41 50 1000 10000 50000 CO XXX 641 39 60000 AG 00000000000 00000 000000 bo 0 0 D 70000000000 DF 1001 0 WA DISKO 1001 5 O00 DF 100 1 RDD 5 00000 0050000000000 000000 8 000000 FA 90000 AR YG 000000000
97. 0000000 0000000000000000 98010lyekugaktu 0000000000 000 000000000 O01 0000 00 1000000000000000 00 OOCCometIOOOOOOOOO0000000000000000000000 BSA amp HSA westem 12 h 58000 0000 00 0 0 SH 000000000 5 000 SCANNING Completed 0000000000 000 0 IP Scanner Controller 0 100 0 0 0 0 00000 132 5 i overexposre OOOOOOOOOOOOOOOOOOOOOUOU 0000000 0000 0000 000 0
98. 000000000000 0000 0000 35000 DATO SI 0000 00000000000000 00 05 5 005 0 LAN TCP IP 0000 000 00000000 IBMPCATAFCOOOOO 00000000 RU H3RHFA 4RHF RINT20000 00 0 0 Rigaku RASA7R Rigaku 00000 35 Rigaku RASA7R Rigaku
99. 1 SMAA 6000080 000000000 51 00000000000 1000 300 C 00000 0 5000 000000000 00000000000 15000 2800 00000 00000 0 0 0000 213 1 05 200010 000000000 2 2 00000000 0035 000 amp Winleb 0 00 0000 0000000000
100. 200000 2 0000000000000 00000 BahCirculaiong Begin 0000000 0 0 00000 3N HCI 5050 OO OOHO00000000000000000000000 80 1001 000008080 3N HCIQ 100 C 00 5050 000 1000 00 0000 80 somoni SI 000000 105 0000000 80 116 50 000
101. ANALYSISOKO 00000 00000 000000 00 O 00 000 220 000 0000 ENTER 00000000000000 O RESTINGI 000000 0 M120 OPERATION STOP YES 300 1 OOO 3 MT 5 0000 0000 0000 030 00 000 21 3g 0000 4180 0 0000000 0000 150 200m 0000 00000 amp 30m mn 0000 18 m 00000 OOO 100 1200 C 950 000 100 1200 850 C 000 100 999 550 C m 00000 600 440000000000000 m lI SI 000000
102. Deee 00000000 Enter 00000 80 0 00 00 00 80 0000800 4624 481 50 O PERKIN ELMER SIMAA 6000 000000 4808 SI 0000 Freunhofer 000 1900 1995 0000 SIMAA 6000 580 00 2 1 OOUUUU ob a 00000 1000 7
103. O ODODO Modrega 188 kHz Ap 8 1 sec Prase 0 8 SuTime 4 min 5 LF 338 3 mT RF 348 3 Power 1 00 md PARAMETER setg 0 0 00000 PAORS BEERS ICS BA ERO Bes gt PARAMETER SETS 0 0 5 00000 K a K 1000 000 00000 0000 Ser Auto 00000 Frog 9 45770 GHz Coupling HPhese Power 1 00 _ SHF tune gt gt gt Tune Frequency 1 lt lt lt Freq 9 449068 GHz Coup ing 81 Phase 577 Power 00000 0 00 nwx 20rw0 0000000 Coupingd CURRENTI 61 0 0 POWERQ InW O00 SF Semifutomatic tune gt gt gt Tune Phase lt lt lt Freq 9 4498 GHz Coup ing Ph
104. 5 2100 950 000000 1 5 11152040 C gt P gt s Tc 210 BAS TR2040S 0 0 00000 BASO 2024 00000000 ROD 5064F 0 0000 43591 2 135 OOO 000000 4153 580 0000 10
105. 000 Cul 100 30 c0000000000 22 00000000 90000 00000000 SI 000000 100 200m0 0000000 000000 0000 0000000000000 950 000 0 dscoo 000 20 105 127 2 00 0 0 90 20 1600 000000 196000 000000000000 0000000060 9 0 Fomcg 120 0000 0000 5 00020C0000000000070C00000000000 105 127 19 90 aa 120 12 3
106. 00000 210000 TOFMS Marix Assisted Laser Desorption MALDI 00000 00 00000 0 00000 0 000 0 000 000 00 0 00 0 0 0 0 lonization Sample Pulsed Laser Matrix 337 nm OOO Laser exposed matrix Probe Tip OO 0000000 Matrix Application Structure Sinapinicacid SA Peptides MW 224 07 Proteins CHsO NOCH OH Alpha cyano 4 Peptides CH C CN COOH hydroxy cinnamic Proteins acid CCA MW 189 04 2 5 dihydroxybenzo Peptides COOH ic acid DHB Carbohydrates Glycolipids MW 224 07 H G yD Polar synthetic polymers HO 2 Time of Flight MS 000 95
107. 0000000000000000000 50 2 10 21 50 10 200 50 1000 200 500 1 0000 RMSQ 0 0 0 185nnq 0 050 200 0 049 500 0 05 0000 000000 1 00000 00 Inm 00000000000 02m 30 000000000000 000000050 0 2140 80 160 32 64 0 125 0 250 0 50 10 20 40 80 16sec O UvO O 000000 OJ 5ABS 0 0 5 000 0000 00 10mm Inm 0000000 2095 LO 0000 10 0000000000000000000000 0000000 NEC981 BX2 SI 00000000 0 0 00 0210 2121 00000 00000000000000 20 1 145 950 000 000 000
108. 000000000000000000000 0 200000000 691 219 502 0 2 PW peak Width 0 49 0 6 4 Abundance 69 1000 0 O O 219 350 00000 502 10 0 0000 5 0 0 180 289 320 0 0 0000000 000000000000 0 100 100000000000000 000008080 000 660 0 83 1 Method EditErtireMehod O 000 00000 0 0 0 0 0 0 000 0 00 0 0000 200000000000000 0 0 000000000 3 Acquisition Andysis TO 0000000 00000 40 0 HP5972J 000000000000 5 0 0000000 0 000 6000 scan O 0O 0000 SMO 000000000000000 00000 0000 0 00000 7 OO OU Solvent Daday 0 00000 000000 00 5 000 Voltage 0 RAD MS Scan Parameter MS Range 0 0000000000 SatTime 0 00000000000000 0000000000 Threshold J 0 0000000 0000 0 0 1090 200 000000 Sampling 2 0 0 00000000000000 00 0 00 0 000 000 0 00000 0 Scavseq 0
109. P1 10 0000000000800 11 REFO 00000 00000009520000000 47410 000 UUUUDUUDUUDUDDUDDUDUUuunuun b M1 Dis c TOP 502 0000000 10 3830000000000000 12 Basing 0 0 0 ATD 13 DATA Reduction YG BA 000 0 0 NL Basing TH RDU 400000000000 OO INT CLR YAD M1 JI 09 0000000000000000 M2 000 Dis SET YZ RS B1 RS INC M10 INCI OK SI 1080 REF OK 15 0000 INF INT 27000 0 00 0 1 00 00000 YAD 41 Yz 00000000000 950000 051 0523 000000000 CHG Msa 0000 PL
110. 000 000 000 0000000000 000 000600 RETURN End SPACE Nett 40 End 0000 0000 0000 00 00 00000 PM VOLTII 00000000600 0 RESPONSE 000000000000 110000 000 00000 00000 00 0 00 0000 00000 DURATION 00 000000000000000 0 SF GAIND 000000000000000 00000000000 00000 20000 0000 00000000000 00 0000 0000000 00000000 CR 000000 0000 00000 00000 0000 OO00000000 F000 30 VISUVG 0 0000 00000 0000 0 0 000 00000 IFLOWIT OOOO 00 000 0 00000 0 0000 0 0 0000 90 FLOWN 0 0 0 0 AUTON 152 O0000000000000 PRESSIG 0 0 0 0 OFFO OO 00 OOO0000000000000M000
111. 0000000000000 000 000000 000000000000000 Jusrfosopleftexsarycytidine O0 0 0000 15 31 9 0O 00000000 texr y inf OO l 00000000000000000 teXsan GOO texsan CR 0000000000000 00 0 0 000 O00 00000000 txsan0 000 00000000000 0 000000000 0000 MSC 00000 24 30 0 Graphics O 0 000 aksroteion 2 0 000000 90000000 0000 00 0000 00 00 00000 0 0 0000
112. chi 18 0 00000000 SANYO 18 50 0000000000 00000000000 0000 000 00000 0 DOS Command 000 0 wing 0 0 O 0 MS windows 9 0 0 O MICROCALO OOO UUUUDUDUUDUUDUDUDDUDUUuunuun 0 5 1 BahCircllaiog OFFI I MC 2 Control Padi TEMPERATURE SHUT RESET 00000 O0 200 000 O00000 0 0000 270 115 000000000060 0
113. 0 000000 00000 DPI 0 0 ON I I J I 5 transfer line 0 0 0 00000 0 0 Detector temp 280 C oven 111 0000 Detector B temp 0 25322 0 IU 00 0 0 0 0 0 0 0 00000 66 4 82 024 00001 10000000 5 0000000000000 2 0000000000000000 00 3 000 000 0 0000 0 00 680 000 0 00000 0 0000 0 0 00 00 00 0 Deector off oven terp off orf injector terrp off 40000000000000000000000000000000000000 00000 5 00000000000 5 000000000000 00000 0 MSD 000 0000 6 0 0 0 000 000 00 0 000000 000 0 0 00 00 0 00000 7 Window 00000 6 OO0000 8 000 Windows 00000000000 00000000000 OKOOOOG 0000000 9 0000 0 000000000 03 00 000 1 TuneMS Standard Autotune 2 160 000000000000 00000600 000000000000 000000000000 6 5 0000000600 4000000000 10
114. 0 0000 Inha 0 000 00000 nn 00 0 000 0 0 0 00 0000 DUUUUDUUDUUDUDDDDUDUUGuUUDUDUUBUDUDDUuuunuun 0 000000000 O Gradation 0 0 220 0000000000000000 300000 0 000000000000000 Radioactive 00000 0 Macua 00 000 0 Profile 7 Tim 0 Supeimpose 00000000000 00 Tang 0 Magnify 00000000000000 Qut lJ 08000 000 00000 0 000 000 000 000000000 000 IUUUUUU UU UU II 000000000 0000000 0000 0000 000000000000000600 133 10 0000000 Youcan lt cess MOdisk 00000 0 0000 00000 ChangeDir os100 im
115. 000 0 00 0 0 0 0 0 231000000000 Vetica Window 0 0 O ANALYSED LIFE TIME 0000 LASER 0 0000 2 0 OO0 FITINGG 000 0 SQUARED 00000 0 0 000 0 0 00 0000 0020 0000000 30 00000 0 00000 139 221 15060 0 0 100 900 81 DECAYO t 10000000 21 0 0000000000 Photon Counting 2711 LIFE 270000000000 SI 000 5 0 0 5 00000 5 0000 22 0 7340000000 Relative counting Time ns OO
116. Acceleration Free Flight Region Region DSc eee eee ee eee Sie ee SUN Detecter Length X1 Flight Time t1 Target OO TOFMSIJI U U Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometer 1 0 0 0000000000 00 Mash 0 0000000000 Caibrany TA buffet 0 1 TFA acetonitril 2 10 0 10 10 prol ul 0 0 0 0 0 10 Manx 1110000000 000000000 a BE peepee Calibrant x Mwe5000 Angotersinll human Substance P ACTH 18 39 Insulin Bovine gt 5000 lt Mw lt 10000 Insulin Bovine Ubiquitin Cytochrome horse 10000 lt M w lt 60000 Cytochrome horse Myoglobin horse Trypsin bovine BSA 60000 lt Mw _ BSA BSA dimer 00 0000 Cadibrent Calibrents 00000 M42H Angiotensin 11 human 1046 192 1047 200 Substance P 1347 728 1348 736 ACTH 18 39 2465 720 2466 728 Insulin Bovine 5733 549 5734 557 2867 728 Ubiquitin 8564 835 8565 843 4283 425 Cytochrome C horse 12360 080 12361 088 6181 048 Myoglobin horse 16951 457 16952 465 8476 736 Trypsin bovine 23311 53 23312 54 11656 77 BSA 66430 66431 33216 BSA dimer 132858 132859 66430 0000 0 0 IMg2HI 97 SH 0000000 0000000000 0 Phamesa Biotec
117. MALDI TOF MSO Bruker REFLEX Matrix Assisted Laser Desorption Time of Flight Mass Spectrometer 0 0 0 MALDI TOF 00000 0000000000000 00000 SCOUT 2600000000000000000 00 OU SUNG 111000000000 59 00 0000 16Hz 00000 100 0 21 2000 300000 gt
118. closed 28 XFER TO INJ 1 9 10 Delivers sample to injector 29 INJECT POS 36 Moves injector to inject position 30 LOAD POS 35 Moves injector to load position PRE PRELIMINARY VERSION 8 14 89 12 13 PM 3 5 Table 3 1 Model 473A Functions continued NO NAME VALVES DESCRIPTION Reaction Cartridge and Conversion Flask Functions Functions 21 through 49 can be activated as reaction cartridge or conversion flask functions 31 33 34 46 48 49 OUTLETCLSD 38 HEATER COLUMN HEATER SENSITIVITY SENSITIVITY AUTO ZERO WAIT EVENT 5 ON EVENT 5 OFF EVENT 6 ON EVENT 6 OFF RELAY 0 RELAY 1 CHANGE R 25 26 27 28 29 30 31 32 CHANGE S 33 34 43 44 Closes the overflow line from the loop Set cartridge temperature C Set flask temperature C Set column temperature C Raises detector sensitivity setting Lowers detector sensitivity setting Absorbance set to zero Close valves and wait Turns event 5 on Turns event 5 off Tums event 6 on Turns event 6 off Changes relay 0 setting toggles between on and off Changes relay 1 setting toggles between on and off Vents reagent bottles Vents solvent bottles Conversion Flask Functions Functions 51 through 66 are activated as conversion flask functions 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 2 2 8 2 2 2 _ PREP 54 2 2 8 2 2 3 8 PREP R4 R4 LOAD R4 PREP X DE
119. 0 00 li 000000000000000000 00000000 005 0 0 0000000 gt CYTIDINE G 000 C X gt MDCYTIDINE CR 20000000000 C X gt CD CYTIDINE CR 00 0 0 00 0 0000 0000 000 C XYCD CYTIDINE gt GETMAS CR C X CYTIDINE gt CTR CR i 000000000 C XYCYTITINE gt OMEGA45 00000 0 0 0 C X CY TIDINE gt CHI90 000x00 00000000 9 C XCYTIDINE gt CHIO CR 00000000000000 PHI90 1801 270 27 0000000 0000 000 x 000 20 L 0000000000
120. 0 000 0000 0 0 00 0 File 00000 Printer seuph 00000000 297 420 0000000 00 0000 0000 PrintPerareter 00000000 000 Preview 00 00000 0000 Ferse 000000000000 0000000000000 00000 oo SAMPL 000000000000000 00 2 0 0000000 SAMPLO 000000 SAMPL Conred ejet 00000000 0000000 5 0 48 00000 00000 1 000000
121. 0 000000 000000 13 IUUUU UU UU UD II 0000 IMBALANCE 0000000000000000 OVERSPEED ROTOR DISK OVERTEMP 00000000000 30 0 0000 DRIVE TACHOMETER 000000000 TUBE LEAK 5 50 0000000000600 0000 000000000 1 000 65 000 1 000 pri 00 0 Bp O 100mm 0000000 00000 2 000 2 2 0000 37 5920 000000000000 0 0 HOLDII 0 300
122. 0 000000 001000 Frontor Rea ALS 0 0000000 00000 000000000 Sample Pumps 0 0 0000000000 SarpleVolume 0 0 0 0 0000 110 0 00010 00000000 590 5 Viscosity OOO 0 00000 00 00 0 0 0 000 00 00 0 0000 00000000 Solvent A B Washes 7 0 0 0 On colum 0000000 Ye No Purge 8 100 00 0 00 0 000 0 1 000000000 2 00000 0000 Time Off Tima 0 Oming 0 0 0000000000 Time 00000 0 Off Tire Onin 13 MS Run Tadg 0000 MSD EM 0 000000000000 00000600 85 MSO 14 SdectReport 0 00000000 Percent 0000 Lib Search Report 0000 Quant Report 000 0 Custom Report MS 15 000 0 sae 5 0000 0000 0510000000 1 000000 2 0 FileName 0 0 0 Operator 0000 Sampe 0000 00000 0000000000 3 0000 0000 0 0 0 00000 0 0 0000600 4 Solvent 1 O0 0000000000000000 0 0 500 o 0000 59 Tind 0000 0000 000 5
123. 00 5 Procedur DeaFile Description 000 00 0000 0 77 5 000000000 0 0 0 Do you wish to this 0 000 000 Enter DaaReducton 0 0 0 0 0 00 0000 00 0 0000000000 000000 000000 0000 SI 000000 0 00000
124. 00 0 000000 kero 00 OOUKBOOO00 10200 00001 1000 0011 0000000000 co 010 0 50 5m 00000 C gt 000000 lt 68 000000 0000 2 6 0 0000000000 0 BBB s 00 0 0 0 0 0000 2 O 1 00 0 0 4 70 0 2
125. 00 000 iv 00000 X RAY 00 00000 20kv 10m 000000 000000000 0000 0000000600 0000000000 000 v 0000 000000000 00 0 0 0 000000000000 0 000 0000 0000 SEARCH 20 25 OOOO 0000000000000000000000000 000 0 INDEX 28 DELAUNEY
126. 000 read Cacuaion 0 0000000 0 00 3 S B n 0000 420 70 0 00 0 20 0000 0 40n 0000000000000000 000 0 O O T Manabe Manabe and H Hatano FEBS Lett 23 268 1972 0 02 04 06 08 10 Time sec Wavelength nm ARMAND PARLOR 7 O ic 330 420 630 nm 12333 RRE b 0 0 2 RLU 2sec MTBTSANFZ FA lt TOHI O 420nm RERH cA n FRAY 1 47 107 1 20 10 pH 5 5 25 C 50 North Holland Publishing Co 17 19891 223 1000000 1993
127. 000 00 000000 OO 0 000000 10 DD 00000000 0000 000000 2 00 000000000 OO H 000000 24 00000000 2701 4000 500 MH4 0000 000000 37 00000000 300 20 0000 000000 4 00000000 OO 0 000000 51 000000000 0000 000000 56 DDD 000 00000000000 0000 000000 5 000 00000000000 0000 000000 8 000 0000 000000 88 000 DDD 000 0000000000000 0000 000000 14 000 000 000000000000 OOOO 000000 122 000 000000 OO 0 000000 130 000 00000000000 0000 000000 1 000 0000000 OOOO O00000 141 000 O 00 000000 147 ABI 47 PERKIN ELMER 473 000 000000 0000000 4362 000000 4363 SI 0000 UUIUDDUDUUDUDUDDUDDUUDUDDUUDUDBDUDUDUDDUDBDUUDUPITCIU DD 00 0000 0000 000 0 0000000000000 Schodde 0000 00000000 0 0000
128. 0000 00000 434 Sao 0 000 SI 950 0000000000 OOOO 0000000000 0000 98000 0000 9200 0 I 0000 000000000 2000 800nm 0000 0 0004 AOD 000000 0 5000000 Ormgoodo 000000000000 0 294 10 10mm 00 42 000 147 211000 wun sh 0 OOOOO0 000 Pessoig 00000000000 00000000000 0
129. 00000 00000 0 PERKIN ELMER SIMAA 6000 0000 190 860 nm 000000000 0 2 0000 2000 C 000 0 10 99 5 2000 AD 40 0000 PERKIN ELMER SI 00000000 IJUDDUDUDUDDUDBDUDUDUUDBDUUDUDBBDBUDUUDUDBDUDBUUDUD06ml 53 SI 000 za cdg 00000000000 Fig 2000000000000g 00000000000 30000
130. 000000 950 000000000 000000 0000000 0000 0 0 0 MOLGRAPH 000 00000000 rasa 1 mg molfs CR 0 MOLGRAPH 0 0 0 0 0 OOUUUUU OOUUUUU 0 0000000000000000000 0 33 SI 00000000 OOO00000000 Rigaku 77 Rigaku 6 00 0 0
131. 118 5 5 010 img 2 2 2 2 0000000000000000 UUUUUUulu 00000000000 ONG CONTROL 0000 OND 20 0 ACC VOLTAGE OFF ONG ACC I IU IU DU U 0 00000 0 2 0000 0000000000000 119 ACC VOLTAGE
132. 2 SI 0000 20 0000 00 00 00 0 000 0000 0000 OOOUCOBr 0000 SI 00 0000000 FABO FOO ESIQ 0 0 2 O 00 0000 000 0000 000000 dectronionizdio 0000060 0000 10 000000000000 00000000 0000000
133. 20 00 5190 0 0000000 F30 OO eee BJO 000000000 O 000 70 0 0 0 0 ease HOMER 000000000 O 69 000 2 0 0000 2 2 0 000 000 000 000 000 000 0000 0 0 UUIUDDUDBUDIIDBUDUUDUDBDBUDUDFOIHDBI 20
134. 50 2 00000000000 pug 0000 0000 SolventA3 Solvet B2 00000 HPLC OO purge 7 0000000000 0000000 000000000000000 0 Mode 610 00000 0000000000000 0 0 000 000 0 U 0 blactoglobulin 66000 00000 0 0000 0 00 00 0000 0 000 Mode 610 000000000 00000 00000000 3 00000 000000000 00000 000000000000 OFF 950 00000 00000 000000000 0000000000000 473A 0000 500 100 pral 0000000 0000 IIC 00000 Mode 610 1 0 2 SI 00000000 4 Gas regulators Liquid aysal display Soh keys Adjusiment for LCD swich Bottle 1 X1 standard Boitle 2 R3 TFA Bottie 3 R4A 25 TFA Bottled R1 5 R2 TMA Bottie6 S4B Acetonitrile 7 52 Ethyl acetate Chemical to Load at Each Bottle Position Sequencer Reagents and Solvents zs i Contents 1 5 phenylisothiocyanate PITC in n heptane R2 12 5 trimethylamine TMA in water R4A 25 TFA in water with 0 01 DTT PTH standard in acetonitrile S4B 20 ac
135. 6060 Arthmeid 0002 0 0000 00 00 0 00 0 0 00 00 0000000 00 0 0 0 00 00 06 00 000 0 00 0000 00000000 p 90 410 00 0 0 0 00 0 0 00000 3 Files Sava loo 000000000 1 00000 0 Power 2 3 000000000 4 950 0900800 000 450 00000 0000 0000 170 800nm 0000000 0 20 0 50 10 2n 00000 0 0 0 00000 0 0019 2070 00000000000 0000000000 0 1020 50 10 200 500 1000 200 500 10000 20000 5000nmymin 2095 1000 0000 144 COO
136. 9 220 0 05090 300 0 04326 CH 10 Sample 2 Date 83 03 20 Tise 14 50 24 _ 0 asec 0 SH 10 0 185 80 0 07424 170 0 05330 250 as 0 04387 20 as 0 1441 100 as 0 07006 180 ss 0 06367 260 0 04370 30 0 1268 110 0 06600 190 0 05226 270 ss 0 043686 40 0 1123 120 0 08372 200 0 06163 280 0 04868 60 as 0 1020 130 ss 0 06078 210 0 05087 290 0 04536 80 as 0 09313 140 ss 0 05888 220 ss 0 05043 300 0 04848 0 3 9 08640 150 ss 0 05667 230 _ 0 05044 222776 SANPLE 1 83 03 20 14 48 47 300 asec 0 2 ABU 10 1 83 03 20 14 50 24 300 msec 0 2 ABU 00000 00000 5 5 00 0 00 00 0 0 00 0 0 0 0 600 00 0 6 A B C D 00000000 711 Filenme SIO 00000000 wat 00000000 0 00000 154 100 0 00 0 21 C D 00000000 30 00000000000 0000 44000000
137. D 0 0 k CD Cotton O Cotton ORDII 000 0000000000000 000000000 1 0 00 0 500 00000 0 2 00000000000 00000 00 1800 20027 10L mn 1700 1801 000 0 50 3 lj 4 000000000000 000000000000 5 000 Power 2 000 6 00000000000000000 00 gt 142 7 0 0 00 8 0 500008080 9 00 000000000000000 00 Basc fanion Interval scan 0 00000 000000000 000 0 Irteva sai SNI 000 00000 Basic 000000000 Basic 00 0000 0 0 Files 1 Mesure 000
138. LIV X LOAD X DELIV S4 LOAD S4 FLASK BUBL DRY FLASK EMPTY BLK 45 FLSH BLK 3 FLSH FLSK BBL HI DRY FLSK HI 7 9 10 22 Pressurizes R4 bottle purge lines Delivers R4 to flask Loads valve block with a measured amount of R4 for subsequent metered delivery Pressurizes X1 bottle purge lines Delivers X1 to flask Measures amount of X1 Pressurizes 54 bottle purge lines Delivers S4 to flask Loads valve block with a measured amount of S4 for subsequent metered delivery Bubbles gas through the pick up tube into the transfer flask Flushes flask w gas and vapors vent to waste Empues flask contents Delivers gas to dry valve block 3 the metering loop and valve block 4 Activate this function before venting the flask Clears the flow path in block 5 with gas Bubbles high pressure gas through the pick up tube into the transfer flask Dries the contents of the flask with high pressure pas PRE PRELIMINARY VERSION 8 14 89 12 14 PM Beckman 77 651 000 00000000 00 0000 4357 000000 4359 SI 0000 600 000x gf Bekra L7 650 000
139. LNI PLNI 0000 00000 PINI OOOO OOOO O00 000000 000 0000 000 0000 Pl 000000 PI O00000 0000000 PI 00000 0 00000 2000000000 GUO0000000000D H 1 0078 C 12 0000 N 14 0031 O 15 9949 F 18 9984 Na 22 9898 Si 27 9769 30 9738 5 31 9721 CI 34 9689 K 38 9637 Br 78 9184 126 9044 79 HEWLETT PACKARD HP59722 GC MS 000 000000 0000000 4153 SI 0000 5 5 000000 GCU 0 0 5 00 00000000000000000000000000000000 0 0 5 00 0
140. LSM GB2000 00000 000000 Speed0 5 Hi vol t ND Offset 0 Speed2 00000 Speed10 00000 save 126 0 H 0000000000000 000000000
141. PQ FormsubB gt FormB 55 C 0 000 000 0 0000 0 20 000 k 1 AF 10 dH dt Eq 1 AHtotal 0 00000 Ahe 0 n1000 00000000000 000 5408 368 0 12 Hiotal O n 0 r 0 663 1 Ps n 0 990 n 2 10 989 n 3 0 065 Wm S Hiest 0 S ee 4 Ti 000 IM 0 10 FormsubB 0 0000 00 FormsubB 0 FormB 000 000000000 113 Microcal 21 OOO 000000 0000000 4161 950 0000 5 5 5 5 5
142. VACUUM 500000 0000600 00 0 2 NOOOOO 0000 5 00000 5 910 0000000 000000000 000000000 35 kgf cm 0 10000000000000 0 00 000000 Num Lock Caps Lock 0000 5 000000000000000 300000000000000 5 6 7 N GAS Controller O0 000 Controller 0 00 1 8 0 0 0 0000 000 8 000 0000000 0000000 0000000 00000000000 000 9 00000000 3 3 0000 1 00000000 NEEDLEQGO OOO 2 00000000 90 3 000000000 5000000000000 100000000 5100 00000 N GAS 00000 4 00000000000000 Shut 0000 0 0 0 5 00000000000000 Shutdown has completed 0 000000 00000 6 3 4 00 0000000000000000 1 0000 3 5 0 GAS Controller OFF 0 00O 2 EVACO OOOO 0 1500 N 00000 00 1500 N 00000000000000N 000000
143. ase 5 PHASE 0 00 PHASED SF Semi ulomstic tune gt gt gt Tune Phase lt lt lt Freq 9 44908 GHz Coup ing Phase Coupling 62 1 14 396 0 aT Z CHARTO OOOO HOLDO ODODO OOOO 0 0 O00 YO SHORTI 0 0 0 ZROD 00000 000 000 YO 5 000 5 0 mgood OOO 5 00000 SEG TEMPOL 1 x 10 4 4 0 0 0 00000000 POWERD 0 nwo 000 000000 OOO OFFO J I OFFO OOO 950 ESROOOOUU009000000
144. dd 000 000 000 0 0 0 00 201 0 0 0 MALDI TOF 0 00 000 000 00 0000 104 MALDI TOF Strategy Protein N Myristoylation Direct Analyses Co translational modification gt NH2 eo CH 2 12 CONH Gly DN a N Myristoyl transferase sf Digested Analyses Peptide N Fattyacylase N Myristoylated Proteins cAMP dependent protein kinase catalytic subunit Sequence Analyses RSV src gene product Sre An Cn oncogene product Sre CH NH CH HIV 1 p17 989 protein Rn Rn HIV 1 p27 ef protein XI Yn Zn 00000 00000 00000 0 0 Strategy e 198210 00 00 00 cAMP dependent protein 0 100 0
145. duction to Thamal 5 Chapman and Hall London 1988 pp 7 22 SI 5 0000000 0 10 500 C mng 01 0000000000 TGA 00000 01nug 0000 200 c mnj 0000 00 1000 5 00000000 DEC PC 466D2LP 110 80 1000 000000000 2 00000 N2000 0 05 70 0 0 000000000000000 0 00 0 00000000 ON 00000000000 300000 0000000000000 0000000000 00000000 00 20000 000000000 0 000000000 00000000 00 000000000 00 000000 5 J I 0 00 00 Sample Temperature progran Display 0 0 00000 0000 00000 0000000000000 000000000 0000000000000 000000 5 000000 000000000 500 20 C 000 0 00000 000 00 0000 000000 O0 0 0 0 00O Real fir 000 0 DSC I 00000 0 0
146. egsro 10 00000 0 00000000 Un 10 0 00 OOOOOOOOU 0000 00 00 0000 unu 000 0 InegeFileManage O00 0800 00 0000 000 D D 1 0 00000000 0 0000000 0 O00000 Aata 00000 gt 00000000000 0 FileMareger 1 0 0 0 Ipm O00 0000 00 00000 50 10000 060 00000000000 200X 400 000000 2001 Gradatior 256 8 bit 0000000 4 134 0 0 50 10 000
147. et 000000 000 0 Calibrant TA buffet 1 10 00 00000 00000 lt gt CCA Alpha cyano 4 hydroxy acid SA Sinapic add DHB 2 5 dihydroxybenzoic acid 0000 0 0 5 CCAD 0 0 0O00 buffer 2 0000 0000 00000000 00000 3 OO 0000 00 0 0 0 1 2u1 00000000 000 SAD 000 4 Microscop O 000000000000 5 Probetip 0000000000 Manx 0000000000000600 Marix OOOO CCA 00000 SA 00000 Marix 0 0 DABO O OOOO TAbuffe 2 10 00000 20 aheroll 3 110 DHBO 4 DHBO 00000000000 w0 0 0000 5 0 0000 0000 6 Microscop O 000000000000 7 Probetp 0000000000 Manx 0000000000000600 Marix OOOO DHB Matix 00 00 0 00000800 AEAMSQVTNTATIM 1506 02 N 600 9 00000 500000000000 0 00 p2 0000000000 Shoji S Biochem Biophys Res Commun 1997 in press 80 00 Bruke REFLEX Marix Assisted Laser Desorption Timeof Flight Mass Spectrometer
148. etonitrile in water S2B Ethyl acetate O Q R3 Tnfluoroacetic acid TFA neat with 0 002 dithiothreitol DTT lt rs F VERSION 1 004 11 13 89 INLET LINE KNURLED KNOB FITTING SPRING WASHER S KEYED METAL SPACER UPPER GLASS BLOCK GLASS FIBER FILTER D TEFLON SEAL LOWER GLASS BLOCK TEFLON SPACER CARTRIDGE HOLDER FERRULE KNURLED KNOB FITTING OUTLET LINE Bld Die PU se Aeg B pwopagan eas Block 1 OOOOOO py 3 s OO O s z s 0000 Solvent A Ini To Waste Gas Regulator MODEL 473A DELIVERY SCHEMATIC R3 GAS PHASE UPDATE Table 3 1 Model 473A Functions YALVES DESCRIPTION Reaction Cartridge Functions Functions 1 through 18 are activated as reaction cartridge NAME functions 1 PREPRI 2 DELIVRI 3 LOADRI 4 PREP R2 5 DELIV R2 6 LOADR2 7 PREP R3 8 DELIVR3 9 LOAD R3 10 PREP 52 11 DELIVS2 12 LOAD 52 13 PREP XFER 14 XFER 52 15 XFER GAS 16 DELIV ATZ 17 DRY CART 18 BLK 1 FLSH 6 20 29 30 6 16 29 30 15 16 29 30 6 20 31 32 6 17 31 15 17 31 32 6 20 27 28 6 18 27 15 18 27 28 6 20 33 34 6 19 33 34 15 19 33 34 5 7 20 33 34 5 7 19 33 34 5 7 20 4 19 33 34 6 20 15 20 Pressurizes RI bottle purge lines Delivers RI to Rxn cartridge Loads valve block
149. h Resource RPC eg NaCl CaCl K H POJ Deteroert eg Tween Triton 5050 Cheotropic agent eg Urea Guanidinium solvernt e g DMSO Glycerol 00000000 DMSOIJ 000 00000 U OU Mar o Mw lt 5000 1 2 prrol ul CCA 5000 lt Mw lt 10000 2 3 prrol ul 10000 lt Mw lt 60000 3 5 prrol ul SA DHB 60000 lt M w lt 5 SA DHB OO 000000000000 00 Manx 000 gt 000 OOO00000 TA buffet 0000 00000 00 O0000 bufed 00000 Intenal calibration 0 0002000000000000 0 00 0 0 0 0000 OTA buffer 0 1 trifluoroacetic acid in weter acetonitrile 2 1 Q Intend 0 000000000000 00000 Cdib
150. munoblotting andysi O0 00 00 00 00 600 00 60 60 QD 00 00 00 00 60 0000000000000000 00 Coomsssie blue G 250 0 00 O Q 000000000000 00 0 typsing 0090 EtOHI O 00 200 00000000000 0 1 0 30 0 l 00000000 li 0000 000 0 00 colum colum A buffer 30 containing 0 1 B buffer 80 containing 0 1 Gradient 0 gt 100 30 mng 00 0 0 MALDI TOF 0 I l 1800 N Myr GSNK M H 1600 1400 615 81 1200 1000 800 600 1047 20 400 200 100 200 300 400 500 600 700 800 900 1000 1100 1200 mz 000 N Myr GSNK peptide Shoji S et al Biochem Biophys Res Commun 2 17 632 639 1995 p 0000 HPLC O00 000 eeto 18 ning MALDI TOF MS 000000 0 0 0 0 0 tetrapeptide O 0 N Myr GSNK peptidd 00000000000 Peptide N Fatyecylese 0 0 00 00 00 HPLC column PICO TAG colunn A buffer 30 CH CN containing 0 1 B buffer 80 containing 0 1 Gradient O gt 100 B 32 ri 0000000000 2
151. with a measured amount of RI for subsequent metered delivery Pressurizes R2 bottle purge lines Delivers R2 to Rxn cartridge Loads valve block with a measured amount of R2 for subsequent metered delivery Pressurizes bottle purge lines Delivers R3 to Rxn cartridge Loads valve block with a measured amount of R3 for subsequent metered delivery Pressurizes S2 bottle purge lines Delivers 52 to Rxn cartridge Loads valve block with a measured amount of 52 for subsequent metered delivery wash Gas through transfer lines 52 to Rxn cartridge amp flask Delivers gas through transfer line to flask Used for manual collection of ATZ extract Gas through Rxn cartridge Delivers gas to dry the fluid path in valve block 1 Reaction Cartridge and Conversion Flask Functions Functions 21 through 49 can be activated as reaction cartridge or conversion flask functions 21 LOADGRAD Loads gradient into pump memory 22 INITGRAD Halts pump fills syringes equilibrates the system at gradient time zero conditions 23 STARTGRAD Starts gradient from time zero 24 STOPPUMP Stops the pump 25 INJECT 1 9 10 21 36 38 Maintains high pressure loop loading conditions and moves the loop to inject position 26 HPINJCLR 1 9 10 21 Clears the line to the injector independent of the inject position 27 HPINJ LOAD 1 9 10 21 38 Fills under high pressure when the loop outlet is
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