こちら - 一般物理学実験のホームへ
Contents
1.2. 1 49 2 43 6 32 5 PC 34 7 PE 28 096 SM 20 1 PS 14 3 PA 2 2 11 1 7290 6 7 0 5 lt 0
3. 600nm 300nm 31 00000000000 po00000000 00000000000
4. 1 0 1 00000000000000 30 DMPC dimyristoyl phosphatidylcholine 17 PC 00000 4000000000000 000 0000 000000000000 0000000000000000000000000 D00000000000 Lg Pe 0000000000000000 000 pretransition T 0 O D main transition
5. 5 0400000000000000000000000000 5 9000000000000000 0000000000000000000 34 00000000000000000 0 4 VV VH HV HH V V 9
6. 45 1 00000000000 DO 00000000000 0000000000000 000 0000 00000 2 1 e 11111111111010 1010111100 11101100001100 000000000000000 0 00000 00 DD O ashift O nm2wavenumber 00000000 0000000000U000U00U000U0 00000000300000000000000003000000000 00000000000000000000000 010000 03650
7. 18500000000000
8. QQ MLV multi lamellar vesicle JO MING 1 111111 11111 111011 111 1 111 1 1 1 1 00 1 1 11 1 SUVI small uni lamellar vesicle 7 D D O B O B D 0 SUV MLV 0 111001 11111 1 0 1 1 1 1 0000000000000 11 SUV U U O U LUV large uni lamellar vesicle I O O WU U U
9. ao photobleaching 21 0000000 FP 63000 00 0 000000 FP 63000 0000000000000 0NQ000000000 eo0000000 FP 6300 000000000000 U U U Spectra Manager J PCY D D D DO D D D D D DL U U Spectra Manager 80000000000 MER Cae te R
10. OOOH 254 0000000000000000000000000001 1 50 250 350 400 420 450 550 6 0000000000000 00000000000035 900000000 RFEJ IJ HI
11. U U Fer Fenn FU U 0000000000000000000000000000x000000 20000 00000 87 00000000000 800000000000 simeco sn OO 000000000000 005 22000000000000 404000 0000000000 0000 Iy t k T cos 0 12 I t k cos 0 sin 0 cos WD 6 13 000000000 000000 0 0000000000000000 D 0 y d0dy E sin
12. 5D 80 C 1 000000000000000000000000 TA 2
13. OU 1 Stokes I D B
14. 000000 UUUUUUDDUUUDUUDD Maxwell DU D D 11010 0 linear polarization D 1 D O 11 1111111011 D O D DO D O D O D O DO U
15. VOU sample cell 3826 Emission Wie Excitation vertical polarization Ir t Ty t Zut 4 _ ly r t E 5 00000000000 090 900000000000000000000 1 Ir t Io exp t T 6 r t ro exp 1 0 7 0000000 0000000000000000000000000000000 0000000000000000000000040000r000000 00000 00000000000000000000000000000r0 20000000 000000000000000000000000 i Ir Dr t dt 8 mat 0 000000000000000000000 1 7 9 00000000000000000000000000 0000000000000 00000000 gt 0000000000000000000 gt 000000 0000000Y000000000 7000000000000000000000 0000000000 200 D ksT 6Vn 9 NO A
16. 10 0000000000000000000010 0000000000000000 00000000000000000001000000000U00000000000 000000000000000000000000000000U0000 400000 O U O Jablonski 00000000000000000000010 0000000000 00000000000000000000000000000000U00000000 00000000000000000000000000000000000000040 O000000000000000000000000000 9 shift 000000 30000000000000000000000 DUU phosphorescence D D D D OU D D D D D D 10 0000000000000000000000000 00000000000 00000000000000000U0000000000000000000000 0000000000000000000000000000000000000000 0000000000000000000000000000000000000000 0 111610 0000000000000000000000000000000000 UDDUDD 00000000000000000000000000000000U0000000 0000000000000000000000000000000000000000 0000000000000000000000000000000000000000 000000000000000000000000000000 G 000000000000000000000000000000000000000 00000000000000000000000000000000000000400 0000000000000000000000000000000U000000000 00000000000000000000000U00000000000000000 00000000000000000000 Gi OOOO 0000000000000000
17. 0000 00000010000000000 00000 0 900000000000000 000000000000000000 5S Q 00000000000 40 00 UDUD HEATER L3 r3 LJ L3 L3 L3 L3 OO CH L3 L r3 vn L3 EJ E3 E3
18. 0000 10 UI IU UU IU IU UU UU UU L 33m000000000 3300 500nm D D DE D 40m000000000 3800 CoO D Ooo OOOO L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 A LJ L3 L3 L3 L3 Lu L1 L3 L3 L3 L3 L3 L3 L3 L3 4 L3 O L3 L3 L3 L3 C3 L3 L3 L3 L3 C3 L3 L3 L3 L3 HUU 000 000 OOO OOO UB 000 OOO a ES ES gt ES 100000000000000000000000000
19. 6 1 47 87 OU 000 2000000000000000000U0U0 000 LP 2 4 6 6 T Tin x10 000000000 0000000 00000000 DNSD DD D DO 21 sucrose 1 I 1 D D D DO D OD D D D 0000000000000 00000 sucrose UUUUUUUUUUUUUUUUUUUUUUUUUU 210 DNSU D D O D O D PU
20. 00000000 UUUUUDUDDUDDUUDUUDUUDUUDUUDUDDUUDDUDUDUDUphoon OOD UUUUUUUUUUUL 000000000 00000000000000000000 000000000000000000000000000U00E WIUOhrODOODOIU eVIcm 0000000 D D O O O O D O D O O OD DO OO OD D 010 cm D DD
21. Ami IJ UD U DOPC O YUU 10 Rh DOPE NBD 0000000000000000 3 DOPED DUDU 100 Gi UUUUUUUUUUU LDUUDUDDDU
22. 3 1000000 112 41 5 09000000000000000 10600 0000000 0 5 610 PP 300000000000000U0U000 240000 00000000000000 1000000000000 00000 00
23. 6 0000 STEXD U g factor anisotropy 0 60000000 GUUIDUUUUUUUUUUUUUDDUUUDUUDUUDUUUDUUUUUD UUUUIUUDDUUDUUDUUUCUUUUDDUUDUUDUUUDUUUDUUUD 000000000000000000000000000 KO 0000 VOOOOOOOO 00000 G Vex 1 ll Inu vex 40 Ivv Vex G vex Tyn Vex Web mmm DCW SaaS 7 GUD D OD D D D D 0000000000000 410000 00000000 0 36 DOIN D D D D D D O D O D O DO D O D OO D OD O HO DO DO D 01 00000000000 a e 0000000000 DPPCI 0000
24. I t IN exp t tp exp 2y t rp 36 y 41 40 3000 Aol N 37 00000000000f 4J0 mc 100000000000000000000000 oco Lia L laa t dt 2 LT 1 Vryexp y 1 erf y 38 4 L D dt 2 ki 2 er f y exp x dx 39 vz Jo uultu Ee nih c ER E IX DEE 2 8 X 58 0f Mte AP Nag gt NS GS ms AN ANS Z SA KY E e SN M ARS Try ANY TIMES JAN SAN M m UL MW AA RSA SSNs Im XR lt ANN U gt sUUUIUUUUDUUUDUUDDUDUUDUU 17
25. 00000 lt co e lt 100000000000 roo 0 2 gt lt 0 4 27 700 1 0000000000 c000000000000000000 0 04000000000 000000000000000000c0000000000000U Ua 54 7 DO0r 000000000000000000000000000000000000 magic angle I D D D D D O D O D O D D D U D O D 4900101080 GO 2D uuum utl ug 000
26. quenched 000 ci
27. DMPC J UU U UU 5 0kJ mol 26kJ mol 1 3 1 0 0 0 DMPCOO 000 14 23 a OD OO OO DMPCO O O O O O O O O O O O O O O diparmitoyl phosphatidylcholine 0 00000357 41 5 C 0000000DMPCOOOODODODOD DMPSO DMPEO DMPAU 0000000 D 000 00 036 495 0 O O O O O O O O O O O O O O O O O O O O O O 0000000000000000000000000000000000000000 ORORORORORORORORORORE PATA A AAA ce 9 4 AT Cl Pg n N MUD E 117 CU 10 20 30 40 50 y c a 2 6 DB nu U 3 DMPC D H D D O D D D w 00000000 000000000000000000
28. UDUUUUUUU ks 0 gt 11 7700000000000 000000000000 VDUDDDD VOODODOUOOOOODOTIU Trm00000000000000000000000000
29. 40 000000000000040 00000000000000000000 000 0000000000000 0000000000000 pDOD0000000000 FP 6300 4G RF 1500 46 00000000 DoO00000 FP 6300 O 100 RF 15000 0 150 0 1900000000 000 DI I UU U U 0 000000000000 Raman Rayleigh 00 0000 00000000000000000000 0000000 000 300nm 0000000 2800 650nm O O Sensitivity High 0000 000 100 D D D O UU 600nm D
30. 0 110 0000000000 1 01 1 1 1 0000000000 00000000000 L3 L3 L3 OO 1 C L3 L3 L3 L3 Ll 5 1 1 COOC COOC O 0000000 40 DPPC DOPCN D D D D O D D poO 0000000000000 U 2 0000000000 10M0
31. 00000000000000 OU OU ELE E ELE ELE LJ L3 F3 Ho OU OU OU OU OU 110110 10111 10 111 111 111111 11111 aniso_vs temp U ODO DO 0D 0000000000 00000000 000000000 4100000000000 0000000000000 1 Iyy 2Glyg T 42 Ivy 2Glyy s
32. gnuplot gt plot lt normal s start X e end X rf1500 txt gt poo00000000 00000000 1 111111110109 I U gnuplot D D OLD OU U gnuplot gt plot lt area rf1500 txt gnuplot gt plot lt normal rf1500 txt lt normal fp6300 txt v 0000000000 00000000 00000 0 OOOO gnuplot NU gnuplot gt set data style Lines 000 pot0 0000000000000U00000000 000 00000000 repoth U UUUDUUUUUUDUUUUUUU 21 normal area raw
33. AUUOU BOUUU O 1 RF 1500 D D 1 FP 6300 O O 2 4 FP 6300 QO 5 6 RF 1500 O O 5 6 RF 1500 O O 2 4 FP 6300 o o o uw Alu Wim 3 Hg me Himpuiu c Hjo e Hip gola OU OU o CC 00 000 18 e UUUUUUUUUUUUUUUUUUUUUUUHUUUU e UUUIUDUUUDUUUDUUDUUUUUDUUDUDUUUUUUUUUDUDUUD poo00000000 po0
34. SUNU D oD pg000 20000000000 000000000000 0v0000000 A DD DO ADO OD DDS drag delivery system I Il O O O O O I O 000 O 0000000000 3 OOo S2 AFAR IEEE D 00 fluorescence D E DO D U UU STO LI OOOOOOOOOOOOOOOOOO 0000000000000000000000U0U0 0 0000010 00000000000000000000000000000 00000000000000000Fark Coodon 0000000000000 a L3
35. VH 35 4100000000 OU OU OU OU L3 L3 L3 L3 L3 L3 L3 L3 VV DUDU D 0 2 5 000000000000 APPENDIX 0 ID OU OU
36. iii P t2 J 1 a rJ OOOOH Ooo OOOO 00000000 JDUUIUDDUUDUUUUUUUDDDUUUUUUD 0000000 00 00000000000000 20mp000000000 16 2 32 4 44 10 D D O D D D D U D O D D B D D O D O DO D D O D D D D 0 010
37. a 1005000000000 OOOO OOOO 0000 0000 0000 0000 0000 0000 0000 0000 0000 OOOO OU OU OU OU OU OU UD un H H 00000000 STM AFMUUU UDUUUUUUUUUUUNMRYU ESR a L3 L3 L3 L3 L3 L3 C3 E3 F E OOO 100000000 OOO 2 O0 OOOOOOOOOCOOC
38. 42 gnuplot gt set data style points gnuplot gt set data style linesp dinesp linespoints UL D UB U D fluorescence 0000000 set title fluorescence x0000000 wavelength D 00 set xlabel wavelength y DD intensity 00 0000 set ylabel intensity si OOOO ten 000000000 gnuplot gt plot lt normal rf1500 txt title RF1500 lt normal fp6300 txt title FP6300 00000 po00007 0000000000000 gnuplot gt set size 0 7 0 7 D D U DD H D 0000 43 ei postscript O 000000000 gnuplot DU O U U postscript 000000000000000000 1 0000000 postscript D D D D O D O D BI U U U gnuplot DI UI UU U UU UI Uu UU I UU L gnuplot gt set output fp6300 ps U postscript D D D D 11111 UB gnuplot gt set terminal postscript
39. gnuplot gt plot fp6300 txt DU plot repot 1 1 UI DU fp6300 ps D postscript O D UD postscript O O O IPr O D O D O O Uu O O Uu 0 0 LU gnuplot I D D D O D Uu D D D D O D 0010 gnuplot gt lpr fp6300 ps U gnuplot 00000000000000 0 gnuplot gt set terminal 1 000 lpr P 44 x X Window system vid GIUUUUUUUUUUUUUUU GUUUUUUUUUUUUU 600000000000 008 t000000 g factor hv txt hh txt gt g txt 000 gnuplot g txt D D D D LI l
40. anisotropy 1 1 1101 1111101 1 raw rawall current directory txt gear 000000000 aniso vs temp 0 3 40 0000 D D D 1111111011 110000 psjoin O U U 1 1111 spec psplot postscript 000000000 rf1500 txt RF1500 00000000 fp6300 txt 6300 H D UD U LL sample_dopc txt aniso vs temp less bin normal home ohba gnuplot README 0000 URFI5000 0 0 FPe300 D
41. 1 33 porci DPPC O U 111101 1 U 100 411 J J 211 00000000 1 0 110019 UDDUDD 000 0000 15 1 PP6300000000000000000 111111 1 1 DHI 000 OOO OO oop ooo ooo oooo coocoo Oog CC Lr OO Co L3 L3 r3 rc OOOH L3 L3 L3 AO OOOH A 000 0000 00000000 LU PUMP 000 OFF UI UU 6 2ml 0000000000000000000000000000 porep JU IL U UU UU I UU I UI
42. 00000 52000000000 D O I UU D H D D uH 1500 30 LJ L3 L3 L3 LJ L3 L3 L3
43. VVO vH HV BHO V V L 4 401 HEF
44. 00000 U Sensitivity 00000 20 MERRY y F 6 237 18 Xe 5 HV Xe 7 7 MI bike UH s TF 0700000 RF1500 0 000 UD 000000000000 1111 1 11 000000000000000 0000 dark current OU
45. iv ss sous oss ess esk WG a v TI eC eC RW p vi reres era ppp wm 41111 os o wes 5 6 7 1 2 1 2 7 H L 1 2 8 APPENDIX DU D D D D DU D TT D D TI l 52 I U IUD O OD I I I J COOC a L3 000000540 LJ L3 L3 L3 L3 E3 O L3 L3 1 CH OU OU OU OU OU OU OU OU OU OU L3 L3 r3 wooo HO E3 J L3 L3 L3 L3 rJ
46. MODO 0000000000000000000 000000 9 4 OO LL Lr OOH L3 L3 L3 L3 L3 L1 L3 L3 L3 L3 L1 L3 L3 L3 OO OO OOH L3 L3 L3 L3 OOo Co OOo Oo OU
47. 2000000000 a DD 3 301 00000000000000000000000000000 00000000 00000000000 00000000000 0000000000 00000000000000 0000000000000 00000000 00000000000 0000 4 OU 2200000000 2x10 000000 c00000000000000 00ONBDO 00000 000000000000 1 D
48. 7 Ll ODO fp6300 txt O O current directory Ll ii gnuplot gnuplot L D D D Uu B DO Uu I uu ui i uu D LI gnuplot 00000 ODUUDDDUDUDIDUIUUUDUUUUDUUDUUUUDUUDUDUULD 00000000000 40 gnuplot gt 00000000000000000000 gnuplot I II UI UI U gnuplot D DU IU UU UU UU UU UU U lI DU directory gnuplot O directory 000000000000 ELLI gnuplot 0000000 0 current directory D home directory gnuplot D D O D D U I DUU U biophys directory D B I D D 1010 101 000000 gnuplot gt pwd current directory 1 2 net s1 home ohba gnuplot gt cd biophys biophys directory gnuplot gt pwd current directory ll Ill ll net s1 home ohba biophys gnuplot gt ls 1 1s 1 0000000 Sep 3 16 25 psplot Sep 3 16 25 rf1500 txt Sep 3 16 25 fp6300 txt Sep 3 16 25 spec 0000 6 000000000000 UUDUUUUUU I DU I UU DIOD UU 0000000000000000000 1101 10 UU gnuplot I D UD 7B D UI total 3 rw r r 1 ohba wheel 48 rw r r 1 ohba wheel 2917 rw r r 1 ohba wheel 807
49. vi vid 000 SUYDUDUUDUUDUDUDUUUDDUUUDUDUUDUD OOU 1 000000000000000000000U0000U00U0U000000001 00000000000000000000000000000000000 DOPE Rh DOPEQ NBD 0 0 0 DOPE NBD DOPED 0 O O O O O O O O O O O 00000000000000 a 0 40m0 DOPC Rh DOPE 100uM 1 0uM b 00 0 40ml D DOPC NBD DOPE 100uM 1 0uM c D 2 10 DOPC NBD DOPE Rh DOPE 1004M 1 04M 0 14M d 0 0 0 2m0 DOPC NBD DOPE Rh DOPE 1004uM 1 0uM 0 24M e D 2 10 DOPC NBD DOPE Rh DOPE 1004M 1 04M 0 4uM 000 2 10 DOPC NBD DOPE Rh DOPE 1004M 1 04M 0 74M D 0 40ml D DOPC NBD DOPE Rh DOPE 1004M 1 04M 1 04M h 0 D 0 2m0 DOPC NBD DOPE Rh DOPE 1004M 1 04M 2 0uM O 100810 DOPC 100uM 300 00000000000000000000000 00000000000000 3o000000000 100ml 221000000 BDU 2200000600 0 36
50. L 300881 D D D D EE 7 DE 7 CE 7E EE CE Uu 1 11 R e L3 L3 L3 L3 L3 OOo OOH L3 rrr OOH OU OU OU 30 0000000000000000000000000000000 100M0 UU R ii N 000000000000000000000000000000 00 600nm 000 00
51. UU 1 10011 S9MPOD ODO OOO D0 0000000000 30 40m0 D 0000000000 00000000000 11 1 G000 1000 20040 000000000000 5000000 3 00000000000000000000U000000000000000000 000000000000000000 09000000 000 0000000000000 TADOBDUU 29 00000000000000000U00000U00U000000000 110004 poo0000000000090D000000000000000000000 50100000000 5 50 1 po00000000 00 a000000000000M mox O ELO B D
52. d 400000 P 1 II h 43 000000000000000000000000000 r 2P 3 P 44 0000000000000000P000000000000 1000000000 000000000 GP 45 00000000000 21000000 C 000 PD DDUg KK cm sec 10 2 51 1 20 x 10 20 2 59 1 18 x 10 35 2 13 1 12 x 10 45 2 85 1 08 x 10 48 T gt 00 1 P25 D 00000 7 UPDU DODo000000007 1lmsp0000000000000000000 OD 00D 000000 0000000000000 0000 O D D D O O D D O D O O 1000 122 20 0 0 1500000 000 Ooo 2 OOO 1 I IN OU HU UIndole DU UU UU UU UU UI DHU WY Ketone UU C O 0000000000000000U000U000000000000000 400000 2000000000000000N00U00 D 000000 00000000000000000000000 5100000 0000 ul 1000000000 MpoD000000
53. w vb hv 0rwxt 000000 00000000 anisotropy vv txt vh txt hv txt hh txt gt r txt 0000000 gnuplot 6 0000000 UUUIUDUDUUDDUUUUUUUDUUDUUUUUUUUUUUUDDUUDD e UUUUUUUUUUUUUUUUUUU po000 0000
54. 1 1 01 0 60 D 0000 IU 0000000000
55. 000 OU OU OU OU OU OU OOO 46 D D D D D U D D O D O D DO D D D D D 017 TU 00000000 1 11 011000101 0 109 00 DOpCH L3 L3 J C3 C3
56. 5 000000000000000PEGOOOODOODUOOOOOODOODUOOOIU 6 00000 0 PEG6000 501 0000000000030 00000 PEG6000 7 5 1 1 SUVIJU UD 8 SUVD DOO 2000 00000 GU SUD 5000000 20011 D D PEGO DDDDDD 0 5 10 20 30 40 0000000000 2000000 DOPCHOUU DOPC 1 D D D 1 1 1 D UU 9 860 D D D D D D OD D OD D D DO D D O D O D UUDUDPEGUUUUUUUDDUDUUUUUUUUDDDUDUUDUUDUD 10 ab suv 1000000 10040 00 U U 0 5 10 20 30 40 po000000002a000000000000000000000000000 OOH 37 11 SdDD00D00 00D 0D DDD
57. CO L3 L3 r3 28 DODO00D0000000003p000000000000000000000 30400000000 U DO DO 0 0 1101111011 11 1111110111 s 0000000000000000 SUVOOOOOOOOOOOO MLV U 000000000 U 53300000000000000000000000000020400000 0000000000000 0000000000 0000000000 TAUUUUUUUU IUUUUUUU L
58. FTP ftp exet 00000000000000 02000000 GUI Graphical User Interface O 00 0 0 00 0 0 0 WS_FTP RF 150 000000 P c000000 0 FFFTP FP 63000 0000 UU O 2000000 LJ L3 L3 L3 FFFTPIOOOOOOOOOOOOOOOO 0000 2 1 I WS FTP9S 0 Profile Name O 0 O cO1 stex phys tohoku ac jp O O sl stex phys tohoku ac jp 0 0 O STEX 0 home directory 000000 0000 co 3000000 10 son I i Uu i Uu I 00000000000 3 O User ID O STEX O U User ID I Password E E U D D D D 0 11111 1 1 local directory D D 00000 remote directory home directory L D UO remote 18 ftp sftp 4 38 WS FTP950 SIII FFFTP O TV RED mF IDO RTV YD 422350 J x2 ABR E 5 5 H au home ohba blophys 2005 03 16 1 2005 03 16 1 20
59. D D D D D D AI molecular weight A J I D O D O D Ul DU HD A OOO U 00000000 e DPH 1 6 diphenyl 1 3 5 hexatriene MW 232 33 H H H C C C H H H Aex 355nm DOU 00 Am 430nmL 00 7 10 54 0000000 UW e 0 80 0 1 D D ED THF e J perylene T dibenz de kl antracene MW 3 Aex 411nm Aem 470nm 7 4 6ns O ECOH MeOH Acetone e 0 0 Rhodamine B MW 479 03 UI 1 I D 1 4 570nmt Aex 530nm 52 e DPPC diparmitoyl phosphatidylcholine MW 734 05 O10 PCOUUUUUUUUUUUUUUUUUUUUUUUUU 1600000 UUUUUUUUUUUUUHUDPPCOOUUUUUUUUUUUUUUUUMHU 41 5 e DOPC dioleoyl phosphatidylcholine MW 786 12 10 180090 100 20 e NBD DOPE N 7 nitrobenz 2 oxa 1 3 diazol 4 YL dioleoyl phosphatidyl ethanolamine MW 906 13 000PE000000000000000000X8o0000000000
60. o ER 1 1 CH P O C C N CH 20 amp 9 S H2 Hz s PC o CH3 o Ha AAA o 9 O H g 0 P 0 6 C N s PE H2 o H2 Ha 9 Ha Zo S S S S 06 1 N AR oho RCN PS o Lip o o i Ha OS S S c o o 9 AVN NUNUNG 0 cH g 0 P 0 H 2 R Cholesterol 4 000000000 DD OOOO 00000 00000 Carbohydrate chains lt Singer Nicolson 19720 0000000000000 000000000000U000000000000000 200000000000 000000000000000U00000000000U0000000U00 nganani
61. 00000000000000000 0000000000000000 00000000000000000 100000000000 000 D 11 FP 63000 000000000 0000000000 mm 000000000 00000000000000000000000000 00 0 D D D D D D 0 FP 6300 000000000000000000000000000 1 00000000000000 80 0000000000 0 0 O 0 PP 63000000 00000 08000000000 000000000000 0000000 0000 000 a 000000000 000000 UD 20400000000000000000 6300000 013000000 0000000000000 0000000000000000000 00000000 0000000002000000000000000000000000000000 gt 000000000000000000000 0000000000000000000000000000000000000000 0000000000000 00002000000000 DD pp 27 OOOOFP 63000 0000000 0000000 00000000 000000000000000000000000 0000 0000 00 00000000 23 000 G 0003040000000 naan AA DE Horizontal O 3000600000000 0000000 RF 1500 U 12 FP 63000 0000000 000
62. 1029 Fa v e v 128 7 n N4 Jo vi 31 Ro 1 To R R00k 000000000000000 0000000000 0000000000000 1 1 1 1 0 1 0 0 UD eu kr E 33 Th kr kr y 32 00000 000000000000 nagd T Tp kr 34 HU D D DL EL E E 1 Taa tp 1 Faa Fa 35 11 1 101
63. 0 0 00000000000 sin cos 00 4000000 2 gt 2 0 2 0 2 11 6 5 OOO al BUUUUUUUUU fe dedd 0000 Iy t k cos 0 cos D 0 Y f a B dodwdadB 19 I t k cos 818 0 20 D 0 y d6dy m 0 21 47 00000000 0 lt 8g lt 270 00000000000 000000 fla Bdadg aide 22 18 21 22 00 19 201 00000000000 hf xad ae Iy t k zcos a 12 23 0 yr ciay Iy t KE jocos a 36 24 111 1 1 1 1 costa co af a du sina sin af a da 25 0 0 poadd000000000000000000023200000000000000 OU Ly Ig 2 30082 a 1 y cip 26 Fa 5 2 20 0000000 0000000 O 0 9 1 0 e 000000000000000000000000000001
64. 0d0dyr 14 47 00000 4000000 1318000000 k k T t cos 0 sin 0 i dy 15 In t E cos sin ado co vay e 16 47 15 0000000000000000000000U00 _ Iy Ig _2_ dini s 17 1 1 U 00000000000 G Fer Fen 13 Fell For 0000080 Fe UI UI I I I UI 00 lt e lt 70 lt 8 lt 21110000000000000000000000000000 OU cos cos a cos 0 sin a sin 0 18 00000
65. BAWARNAY v h Gl 1201152 1711877 G2 UM 21115 1500 mm 1500 45 mm 5 2 3Z22 76 2 PMT HBF REE 850 2270099 MO M7 IRR 6 1 FP 63000 00 0000000000000 00000000000 000000 000000000000 Ex 0000 00000000000 DODOD DDD DDD 000000000000 DODOD 000000 000000000000 00000 000000000000 1 1 0000000 000000000000 00000000000
66. 0 UUUUUUL RF ISOO 0000000000000 130000 000000RF150000000000000 PEDD DN 0000000000000 DOdOD DD 00000 00000000000 WHOA EX GOTO O SHUTTER EX350 0 0 019 copy EM GOTO 2 2 3 L mE OC e CB 6576 Gora Co KDC AUTO JE QE 03 erum SIART COG X RETURN aar ENTER Mn 6 CE AUTOZERO 2 START STOP 0 13 RF 15000 0000 0 14 RF 15000 00000 RF 1500 0000000000000 I80000000000000000000 0 1900000000000 00000 00000000000 4000 000000000000000000000 F0F00000000000000 0000000 0000000000000 U U RETURNU HB D D 0000000000000 000000000 000000000000 DOdOD 0000 0000000000000 24 UUUUUUUU U 13 EE EX350 EM400 8 1 AKEN KA nn 10 d EX350 0 1500000000 MENE 625 O RA KR HERH LT BARRE 1 EMORI VS m 300 400 LOMBA 3 KAUR E PESE nm 350 450 49m HIGH 4 Av ABE FAST ETE EES 5 EPR 1000 00 6 70 y 3 HID ON 0 0
67. 0 LU TAM TERU TES 150690 EmA HEI HEEE 80 15 RF 15000 000000000 000000000 0 190000000 84 0000000000000 00000000000 0000000000000000000000000 0000000000000000000000000000000000000000 0000000000000000000000000 H0000000000 0 19 000000000000000000000000000 0 RETURNOOOOOG 0000000000 000000000000000000000000000000000000000 00000000000000000000000000000 AUTO ZEROD DDD 0000000000000 000000000000000000 START STOPO D O O O O OO O O O O O O 0 000000 1700000000000000000000000000000000 000000000000000000000000000000 EX350 EM400 0 021 27 AWU 2 1 SPECTRUM 2 ANTHRA 17 RF 15000 0000 O 18 RF 15000 00000000 25 MUTT oe 1207 ERIN EK HABLA hy KOF ED 2 NE F DICU 08132 TANG AJ 0 19 REISOOH D D UI D D D PED D DD UI OB D mm PCOODO 000000000000000000000000000000000000000 0000000000000000000000000000000000000000 000000000 O 90000000 40000000000000000 6 90000000E30000000000000000000000000000 0000000RF1500000001400000000000000000 000000000000 40000000000000000000000000 000PC00000000000000000000RS22C00000000000 0000000000000000RF15000000000000 00000000 000000000RF1500v 0000000000000000000000 19000 000000000000000000 N0000000000000000000 00000000000 Wrdows00000000000000 000000000 0000000000 081500 0 RS22C000000000000000000 00000000000000 Waiingbaag 0
68. 00 0000000000 DU e UUUIUUDUDDUUUDUUDUUUDUUU po000 00000 po0000 e DODO DO OD DDD DO DD 0000000000 00000000 porc D D D OD D D OD D 111111 DO D O D D O DO D 00000000000 e UUIUIUUUDUDDUUUUUDUDUUUUUUDUUUDUUUUUUDUDUUD DOPC SUVD O D D 84 OU UUUUUUUUU 1111 1 1 UU 6EP 6300 0 0 0 00000000000 00000000000 x ed00 000000000000000000000 1010 0 D 000000 000000000000 000000000000000000 000000 000000000000 0000000000 0000000000000 2 BD 000000000 00000000000 000000000 DODDO0D 0000000000000 000000000000 000000000 0000000000000 00000000000 UID L1 L3 L3 L3 L3 XC 19 owes L S R 72771500 M2 M5 M6 Fm M 8 M3 M4 AR 51 08 V y F S2
69. 00 0000 S O 2 NO CHACHA y H 07 6 0 6 Q 9 1 A CH3 CH2 7 CH37 6 0 CH G O P O G G N oo 2 O 2 Aex 460nm Aem 534nm e Rh DOPE MW 1285 11 UL NBD DOPE 000 00000000 000000000000 Aex 530nm Aem 586777 53
70. 00 12 22 2001 08 10 D Ur nan 4 pete 2002 11 15 Dirlnfo C ASCII 150 Opening ASCII mode data connection for bin ls Received 448 bytes in 0 1 secs 40 00 Kbps transfer succeeded 226 Transfer complete e a Auto E L UL 555 Bytes Binary RAR 34001860 Bytes D JVZE 33682 77M Bytes 45774 00 FTP 00 STEXUUUUUUU 20 sub directory ODO directory U U U U remote directory D D D D DU DU D wS FIP H D active OO 00000 directory J D D DD O O UU D O D D OD D D D UU l U U D directory 0000000 5 100111 D 1111 D O D O D DO D D D D O D D O DO HD U wS FTP 0000000000000000 000000000 6 00000000000 Cos 2 gnuplot 1 D D D D D O D D D D D 1 gnuplot gnuplot 7000 00000000 UNIxXpo0000000000000 Ad000000000000 0000000000000000000000000000000U 0000000 OU STEX loen 00000000000 000000000000
71. 000000 0000 home ohba gnuplot setup sh rehash bome ohba gnuplot setup sh0 O0 000000000000000 D home directory O O bin biophys directory D D O O O 0 login directory HOME 7 29STEX bin directory directory 39 normal area area normal s start X e end spec calc Yu spec 6462 DO DO OD DO DOY AD 101 1 nm2wavenumver 00000 000 1 1 1 1 B 11 xshift 1 111111 11101011001 01 1 g factor
72. 000000000 fada 5 sin da costa cos asin eda 5 28 7 1 1 011 00 0 O lt r lt r ro gt 0000 05 lt lt 0000 29 000000000 0000 00000 00000000000 RUUUUUUUUU 11 0 9000 In 4 J Fa v ea v dy 30 0 kr A 28 z2n NAR6T N4D BH B D U U 1 7E I UI TU 11111 11111 111 1101 11 1111 111 OD OD 000000000000 000000000 000000 690 uU utu f 00 70060 90001
73. 0000000000 00000000000000 a 190000 3000000000000000 000 0100000000 12 44900000000000000000000 00ByeCom 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000 RF 15000000000000000000000000000000000000 0000000000000RF150000000000 00000000000 26 000000 OOO stock solution 000000000 Q stock solution D D D D D D Uu D D O DO D O D D D O D O D 0 0 20 4A4 0000 NS50ms m DU 10mM 100uM 0000 0000000000000 U
74. 000000000000 0000000000 00000000000 Dd 0D 0000000000 0000000000 0000000000000 000000000000 00000000000 00000000000 OOOO vid
75. 0000000000000 00000PEG10000000000000000000000 ca 0000000 ca 0000 PEGOO00000000000 0000000000000000000 Gi 40 0OOOOOOOOOOOOOOOGO 300000000000000000 N
76. 00000000000U000000000000 10000000000 00000000000000000000000000000 10000000000000000000U00000000000 000000000 00000000000000300000000000000U00000000000 0000000000000000000000000000000000000000 100000000000U00000000000 00000000000000000 000000000000000000000000000k0 40000 00000 DI ky ky kar 1 A OOOO T ky knr 2 10 2000000000 r 6 00000000000000000000000000000000 po000000 OD OD DDD 0000000000000 U 000000 0000000000000 00000000000 DO DO 0000000000 00000000000 0000000000 000000000 2000 v 00000 00000 00000000000 000000000 0000000000 0
77. 000000Y000000000000000 0000 000000000000000000U0 a in Heptane Excitation of acceptor III Extinction coefficient cm A rsuaqur 22U92S9JOn 300 520 0 Seo 300 400 420 440 300 320 340 Saf 380 00 23 Wavelength nm U 22 000 Dm000000000000000 0000000000000000000 49 DO t nsec Ti nsec J cm mmole x 1019 Methanol 5 3 5 6 1 5 Ethanol 5 6 6 5 3 0 Dioxane 3 6 5 4 13 0 Ethyl acetate 3 3 4 7 12 8 Ethyl ether 2 1 4 5 30 0 Heptane 1 1 2 8 60 3 50 DUDU 21000000000 0000000000 000000000000 L 200 00000000000 0000000 Bpo000000000002000000000000000 100000000000000O0000000000000U0U0U 30 4 10000000 6 Principles of Fluorescence Spectroscopy J R Lakowicz Plenum Press 31 8 APPENDX
78. 00040000 1 8 E HL ORO 82 00000 O MIN cs BAS AS aa ts O dae i bildi D UNAU e ers assess ee o 7 8 ur 0808 ale ede 11111 nc ch dece 0 ae u e DO BUDU WENNER EE HUDBU Yd YR ss G 0 2 0 0 20 kd ER ED BOGE gt lt gt gt orice techs arene su eeta Tk OF 7 OF 22 20 II o DEDE esre eea EE sa bag dug ti iu N ee asa Kl oste bli 1111111 11111 serice ava sss loa g so m 1111 sass sss sass asas 0007 sss erresires sme k o G Fe Fm 111111111 2 232 sasa puas ws bd UR RAOR ED oe 3 00000000 DD DUEB EE ET e gu 002 aid Ale wk HHHBILBBBHUBBHI gt gt gt 4 6 na td vu EE asi e ech i GREE RR EX UK d kl 11 Oe ss ee oe ss et eo Re eee PN PPO DB LB otek sss oss dol kordad HDD sass oa xad S rib e H 1 II 11 ED TEN O POS 84 U 1 2 3 4 ss00000000000 0000 STEXQO FTPHOD DU 6 0000000 po000 B LL DB ii s cart dama OI ch
79. 1 rw r r 1 ohba wheel 94 gnuplot gt directory GOOG 111101 11 1 1 0000 gnuplot gt plot lt raw rf1500 txt UU iii OU OU sraw rf1500 txt DD D pro D I UI 0 DU raw rf1500 txt 00000000000000 0000 poct000000000000000000U00000000 0000000000000000O0rawall0000000 00000 0000000000000000000000000000U0000 oy0000 xt 0 0O0O0OODODOOOOOODUODOOODOUD raw 1 0 9 15001 UO U 000 tx 00000000000000 mawal 00000000 000000000000U00000 U U prog normal 00000 po0000000000 41 raw D 000000 20000000 00000000000000000000000 normal 1 0 0 00 0 0 0 00 0 D D enupletr HD 0 LH U D gnuplot gt plot lt normal rf1500 txt
80. DDUUDUUDUDUDUDDUDDUDUDUUDUDUUDUODDUOD UD 27 111 1 00000 0000000000000 icm etu HJD L3 L3 L3 L3 L3 a L3 L3 L3 L3 mm L3 E3 i LJ L3 L3 L3 oo ao L3 L3 L3 L3 L3 1 r3 L3 L3 L3 L3 L3 41 cc i LJ L3 L3 L3 L3 Oooo AM A ad OOOO OOO
81. INI y DGI AND bv oq BB JASCO Au Bg 37 76781 ris ERR Ble Z re ONL 3 UF ALL TIE Te 0 9 FP 6G00 0000000000 8 Spectra Manager LJ O O G UUUUUUUU 00000000000000000000000000 D Spectra Maneger D 0 0 8000000000000000000 200000000 090000000000 Exo EmO0000000000000000000000 0000000000000000000000000000000000000000 00000000 KO 0101 0 00000000000 00000000000 0 0000000 00000000000000000000000 00000000000000 000000000000000000000000000000000000000 0000 O 1000000 0 0 10 0000000 000 DOK 000000000000000000000 00000000000000 s 000000000000000000000 2 0000000000000000000000 ECH me 000000000000000000000 MEN 0000000000000000000000 ege ALT p swm 22 0 10 FP 63000 000000000 po000 OOO 0000 0000000000000000 90000000 000000000000 00000000000000000 0 00000000000000000000 000000 000000000000000000000000000000000000000 0000000000000000000000000000000000000000 ii 1 0000000000000000 C 00000000000000000 00000000000000000 gnuplot D perl 0 D 000000 0 0 00000000000000000
82. OBOOOOOOOOOOOOOOOOOO 3 Fj F OOOOH L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 L3 82 000 00000 orenllUDUUDUUUUDDUUDDUUUD UIUUUUUDUUUUUDUUD 0001000000000000000000000U O hydrophilic 00000 11 101000 hydrophobic D D D D O UU O D 1 BUD U amphiphilie D D D D D D O O D O D D O D D D D O 0 1000000200000
83. OD L 10000000000000000000000000U00 010000000 12 1 000000000 PEG6000 PEGUUUUUUUU 5 STEX L UO ped U I D OU ODO DO DO Uu In UO D HE DIU D DI C U STEX I STEXOO 0000 1 FTP File Transfer Protocol JO HFPA UUDUUUU User DUU UUDUUUUDUUUUDUDDDUUDUDUUDUUDUDU userID 11 ODO ADO HMMM Windows UD D D
84. OOOOOOOOOOOOO e ocr OOOH OA COOC 0 0 00000000 sooo of 0 ooo SEL pa E 216211 054 2 021 ET EE ee a 07 o o 5152 ESCO Ur L3 r3 L3
85. e Bode k trat kn eh k es 11 sS ess mwa UR mb 7 GOR iv IR TA A e a 1 U U stock solution H HB 11 V sss sss ss d dy oan r s ORC aom a DADO P ht m ASO SSF Oe E s i EA D gt gt criar e Yo ka DES RRE a LOURDES sy and oo 8 6 Mee rg vr PTT PP EE 1 D e 4x a 1 DPPCO UU 0 HE DE v age hU YE UOUUUUOUDOPCOUUUUUUUUUUUUUUUUUOUUUU HD 552 2 vi 111111 1 11 11101 ask dree edo deg o Bind 9 6 es couplet UUUUUUUUUUUUUUUU 22 2 i a9 5 654 ii SAUDIS J sess as s a Z Z s s S ho a su Gai
86. em 1 U D OCOOOOOOOOOOOOOODOODOODOODOO 0 344066 0 1 0 01 000000000000 0000000000000 DD 00 0000000000000 L 0010 UD 002000000000 000 0000000000000000000000 DODO DD 0000 000000000000 UD 3 O D 1 1 D D 0 D Snsec I U U U APPENDIX O
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