取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. Li ornithine dehydrogenase PEST 6 Short life SLG 5 4 so I c s c Pes ST 6 SLO so LLL 65 SLO PEST Eg SLR sn EE co Tao eE 1 S MEE hr 2 Short life 3 SLO SLO PEST SLO SLO PEST CHO MultiReporter Assay System Tripluc hr Detection Reagents A SLO SLO PEST2. 1 Green 550 nm Orange 580 nm Red 630 nm
3. Code No AB 2500 1 3 MCA 560 nm 560 nm LP Filter 1 600 nm 600 nm LP Filter 2 SLG SLO SLR 560 nm LP Filter 1 600 nm LP Filter 2 SLG Filter 1 Tig SLG Filter 1 Filter 2 T2g SLG Filter 2 SLO Filter 1 Tio SLO Filter 1 Filter 2 T20 SLO Filter 2
4. M2 JUEODEBHI VX67vtt D luciferin 1 3 1 3 1 2 3 Short life MRV 501 502 503 511 2 MultiReporter Assay System Tripluc SLR R primer Size Code No ee a ah 20ug MRV 101 s DR Md
5. 2 Fo SLO SLG PCR PCR 2 PCR Denature 98 C 10 5 DMSO PCR PCR p 7 SLR SLO HSL SLG Splatt ak edid NEF JP a Aan E ME NEG CO Endotoxin
6. pSLG SLO SLR SV40 control pSLG HSVik conirol in vitro gt 4 in vivo gt D luciferin D Iuciferin Detection Reagents Lysis Solution Assay Reagent 1 SLG SLO SLR SLO SLR SLG SLG 2 2 SLG SLR
7. 2004 333457 x 2 560 nm LP Filter 1 600 nm LP Filter 22 SLG SLR SLO SLR 600 nm LP Filter 2 SLG SLO 560 nm LP Filter 1 9 2 3 ARVO 510nm 60nm Filter1 20595nm 60nm Filter2 660nm 100nm Filter3 EF SLG SLO SLR Q Filter 1 Filter 2 Filter 3 SLG Filter 1 Tig SLG Filter 1 Filter 2 T2g SLG Filter 2 Filter 3 T3g SL
8. RNA Polymerase II 3 200 250 bp RNA Background reduction signal AATAAA 2 SV40 early poly A signal 7 pSLG pSLO pSLR SLGOR F primer Code No MRV 401 p 7 4 1 SLG SLO SLR SLG SLO SLR pSLG SLO SLR test PEST PEST pSLG SLO SLR PEST test SLO SLR TA Cloning Kit SLO SLR TA Cloning Kit for KOD
9. pSLR SLR R primer Code No MRV 403 5 otegaggtdg acggtatoga taagotta6g cgtgotagdg catgcagate tactagtose CcggatatCg aattcotgea Xhol Sall Clal Hind I Mlul Nhel Sphl Balll Spel EcoRV EcoRI PstI 90 100 110 120 130 140 150 160 gcccaccacc atggaagaag agaacatcgt gaatggcgat cgccctcggg atctggtgtt ccctggcaca gccggoctgc E ion GCA CTTACCGCTA GCGGGAGCCC 17 agctgtatca gtccctgtat SLR R primer pSLR test 1 Hind pSLR PEST test 2 Pst SLR pSLR test 3 MultiReporter Assay System Tripluc Code No CLX 101 MCA Code No AB 2250 ARVO
10. 3 x 3 Ctrl Shift OK 3 x1 MMULT 3 47 x 3 3 T x 1 2 Ctrl Shift OK 10 5 1 pSLG test pSLO test pSLG test pSLO test Multiple cloning region 1 77 SLG SLO gene 91 1722 SV40 late poly A signal 1736 1961 pSLG test pSLO test 4225 bp SLG p lactamase Amp r gene 2055 2915 background reduction signal 3752 4225 SLGOR F primer 3968 3992 SLGO R primer complementary 105 128 Notl 1729 greci ac 1962 BamH 198 Sac Il 1968 Sma 1974 2 pSLR test Xhol 1 Sall 7 Clal 16 Hind 22 DE Kpn13755 ix pSLR test TA Bo 46 Multiple cloning region 1 77 Poor V 64 SLR gene 91 1731 REOR ke SV40 late poly A signal 1745 1970 pSLR test p lactamase Amp r gene 2064 2924 background reduction signal 3761 4234 4234 bp SLGOR F primer 3977 4001 SLR R primer complementary 108 130 Amp n Poly A mua Sca
11. ctcgaggtcg gCCCaCC4CC agctctacag gccgacatcc gtgcagcgag acagctacac acggtatcga atggctaacg ggccctgacc tggagaacag aatagcacca Cgagcgggag taagcttacg agatcatcct aacttctcct ctgtagactg tcttcttcta ctgctggaga cctoctctgg gacccoctgt cacaaccctg tcgagaagta tacgacctgt gctgaagatc tgaagaccgg cctaacaaga ggccatcgac ggctgaaaga atcaaggatg caagagcctg tcttcgtgga tccaagctta cgggatggac acatgataag gatgctattg ggttcagggg gatcctcaaa attcaacatt gaaagtaaaa agagttttcg g acgccgggc gcatcttacg ttctgacaac tgggaaccgg caaactatta gaccacttct atcattgcag tgaacgaaat tactttagat atcccttaac tctgcgcgta ctctttttcc cacttcaaga gtcgtgtctt cacagcccag gaagggagaa cgcctggtat ggagcctatg cctgcggtac gaacctgaaa gcatcacaaa catgtctgga cctgaaacat tcacaaattt gtctg cgtgctagcg gcacggcgcc tcctgaggga gctaagtgct CCCCgtgatc ccctgaacat 27 caccaccggc tcggcaccag tcctacttca Cagaatccca ccagcatcag ggCggCatcc ctccaccggc gaggcgagct aaggagggct gctgatcaag ccggcgtgac accgagcagg Cgacatcccc gccatggctt cgtcaccctg atacattgat ctttatttgt gaggtgtggg tatgtatccg tccgtgtcgc gatgctgaag ccccgaagaa aagagcaact gatggcatga gatcggagga agctgaatga actggcgaac gcgctcggcc Cactggggcc agacagatcg tgatttaaaa gtgagttttc atctgctgct gaaggtaact actctgtagc accgggttgg cttggagcga aggcggacag ctttatagtc gaaaaacgcc cgatcataat cataaaatga tttcacaaat tcataatcag aaaatgaatg cacaaataaa catgcagatc aagcccaggg ggccctgatc acgagaacta gccgcoctgt ctc
12. 8 1 2 3 4 5 6 Nakajima Y Kimura T Suzuki C and Ohmiya Y Biosci Biotechnol Biochem 68 948 951 2004 Nakajima Y Ikeda M Kimura T Honma S Ohmiya Y and Honma K FEBS Letters 565 122 126 2004 76 5 15 2004 7 8 453 455 2005 Nakajima Y Kimura T Sugata K Enomoto T Asakawa A Kubota H Ikeda M and Ohmiya Y Biotechniques 38 891 894 2005 Li X Zhao X Fang Y Jiang X Duong T Fan C Huang C C Kain S R J Biol Chem 273 34970 34975 1998 7 Andrew G B Joanna B Cameron S O and Peter N C Plasmid 44 173 182 2000 31 9 Code No pSLG pSLO pSLR 3 SLGOR F primer 200 pmoles MRV 401 zu pSLG pSLO 200 pmoles MRV 402 SLGO R primer zu 5 pSLR 200 pmoles MRV 403 SLR R primer r z 7 Ee MultiReporter Assay System Tripluc in vitro 100 MRA 101 Detection Reagents MultiReporter Assay System Tripluc in vivo
13. SLR Filter 1 3538 3 Tir SLR Filter 1 Filter 2 T2r SLR Filter 2 3 SLG SLO SLR G O R F0 560 nm LP Filter 1 F1 600 nm LP Filter 2 F2 FO G O R F1 T19 G T1o O T1r R F2 T2g G T20 O T2r R F0 F1 F2 G O R Tio T2r Tir T20 FO T20 T2r F1 Tir T1o F2 G Tlg T2o Tir T2g Tlo T2r Tig T2r Tir T2o Tio T2g G2 Tir T2g Tig TAN FO T2r T2g F1 T1g Tir F2 Tig T20 Tir T2g Tlo T2r Tig T2r Tir T20 Tio T2g R Tig T20 Tlo T2g FO T2g T20 F1 Tio Tig F2 Tig T20 Tir T2g Tlo T2r Tlg T2r Tir T2o Tio T2g G O R
14. 2 pSLG test pSLO test pSLR test pSLG PEST test pSLO PEST test pSLR PEST test 7 Stt high fidelity PCR KOD Plus Code No KOD 201 KOD Plus Ver 2 Code No KOD 211 PCR D pSLG pSLO pSLR 3 TSLGOR F primer Code No MRV 401 SLGOR F CAA TGTATCTTAT CATGTCTGGA TC 5 aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgctcga ggtcgacggt atcgataagc ttacgcgtgc tagcgcatgc agatctacta gtcggccgga tatcgaattc Xhol Sall Clal Hind WI Mlul Nhel Sphl B
15. 20ug MRV 102 20ug MRV 103 eee arg wevaw pSLO SV4D control Hg June se arg wma n ET me ww Sot Eua A ee 20ug MRV 501 en oes fg a a 20ug MRV 502 S sad 2 20ug MRV 503 MultiReporter Assay System Tripluc 22 8 2724 v Code No pros ae pSLG pSLO pSLR 200 pmoles MRV 401 Mi X UNS pSEGIDSEO el 200 pmoles MRV 402 pSLR FA 200 pmoles MRV 403 3 MultiReporter Assay System Tripluc 1 MultiReporter Assay System Tripluc SLG 550 nm SLO 580 nm SLR 630 nm 3 BOIL 1 SLG Green Light A max 550 nm SLO Orange Light max 580 nm Red Light A max 630 nm SLR o ATP O Oxyluciferin AMP PPi CO Mg D luciferin SLG SLO SLR 1 SLG SLO SLR 450 500 550 600 650 700 750 nm 2 Short life
16. MultiReporter Assay System Tripluc SV40 Early Promoter Enhancer pSLG SLO SLR SV40 control Simian virus 40 control SV40 SV40 COS 1 COS 7 SV40 large T antigen HSVtk Promoter pSLG HSVtk control Herpes simplex virus thymidine kinase HSVtk SV40 SV40 late poly A signal
17. 1 5 pH lt Stable Luciferase 2 3 MultiReporter Assay System Tripluc R1 3 DIDHIHKARNIKIL nm SLO 580 T226N SLR 630 3 f IM Code No CLX 101
18. 20 mg MRL 101 D luciferin 20 mg x 5 MRL 101X5 High Fidelity PCR k KOD Plus 200 U KOD 201 High Fidelity PCR KOD Plus Ver 2 an ROP et Blend Tag 7 Sf hi Taq 02 PCR Hot start 250 U BTQ 201 Blend Taq Plus Mi A7 50 LGK 101 Ligation high Competent high DH5 w Dela AGA MS PCR PIK 151 InsertCheck Ready InsertCheck Ready Blue 100 PIK 251 lt gt 32 38 34 HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech osaka Qtoyobo jp URL http ww
19. 2368 9 Sspl 5 ac 1971 BamH 198 Sac Il 1977 Smal 1983 3 pSLG SV40 control pSLO SV40 control Xholi Aat 188 Apal 355 pSLG SV40 control pSLO SV40 control SV40 early promoter 7 207 SLG SLO gene 276 1907 SV40 late poly A signal 1921 2144 Poly A Kpn 14174 N lt Poly A SV40 enhancer 2145 2389 B lactamase Amp r gene 2483 3343 background reduction signal 4180 4653 pSLG SV40 control pSLO SV40 control 4653 bp SV40 Poly A Enhancer Sma 2402 4 PSLR SV40 control Kho 11 Poly A Aoife Aat 188 Kpn 4183 ti x pSLR SV40 control SV40 early promoter 7 207 SLR gene 276 1916 SV40 late poly A signal 1930 2153 SV40 enhancer 2154 2398 B lactamase Amp r gene 2492 3352 background reduction signal 4189 4662 pSLR SV40 control 4662 bp SV40 Poly A Enhancer Sca 2796 Xba l 1917 Noti 1923 Ssp 2472 5 pSLG HSVtk control Kpn I 39597 ori X pSLG HSVtk control HSVtk promoter 7 234 SLG gene 304 1935 pSLG HSVtk control 4438 bp SV40 late poly A signal 1949 2174 B lactamase Amp r gene 2268 3128 background reduction signal 3965 4438 SLG Xbal 1936 Ssp 2248 Poly A Noti 1942 ac 2175 BamH 219 Sac II 2181 Sma 2187 Poly A 6 pSLG PEST test pSLO PEST test Apa1170 pSLG PEST test pSLO PEST test Multiple cloning region 1 77 SLG SLO gene 91 1719 SL
20. 5 10 10 9 9 8 8 7 7 o m AP1 SLO m AP1 SLO P 4 NFkB SLR 4 NFkB SLR P 3 3 BEN am H 1 1 iw oe H 1 Bj H H Hi RER z lt lt AS S s e 8 AN mi e Y we SU lt ys SO S lt oq E 4 Short life AP1 NFzB pSLG HSVtk control HeLa S3 3 1 3 MultiReporter Assay System Tripluct test control SV40 HSVtk control
21. agccccctac tcaagagcga cactccggcg gggctaccag tgcccgacga atcgactaca cgccaccggc cccagacatg tttgtgatgc tttcaggttc cggagaatgg gatgcatgct ctttgcatac gcccggggga gtatgagtat acgctggtga gatccttgag cccgtattga acagaaaagc caacttactt ttgatcgttg acgttgcgca agttgcagga ctcgcggtat actatggatg ctcatatata tgaccaaaat cctttttttc gctaccaact taggccacca ggcgataagt ttcgtgcaca cgcttcocga gggggaa4Cg aggggggcgg tgttctttoc ctccccctga aagcaatagc tatcttatca cccctgaacc caatagcatc cttatcatgt gaggtggtgt cagcgtgatc ccgccaccgt tccaagaagg ggaggatatg agccaaggga gtgctgacoc ctccatcctg tggtgctgct cctcctgtga cggcgcccct tgaccgagac gtgcaggcta gatcatcatg acgtgggata gtggccccog ggccgccggc tcgccgagcg aagctggtga ataagataca tattgcttta agggggaggt gcggaactgg ttgcatactt ttctgcctgc tcctcaaata tcaacatttc aagtaaaaga agttttcgcc cgccgggcaa atcttacgga ctgacaacga ggaaccggag aactattaac ccacttctgc cattgcagca aacgaaatag ctttagattg cccttaacgt tgcgcgtaat ctttttccga cttcaagaac cgtgtcttac cagcccagct agggagaaag cctggtatct agcctatgga tgcggtaccg acctgaaaca atcacaaatt tgtctggatc tgaaacataa acaaatttca ctg cttacgccga tccgtgtgca gaacgacagc ccatcaagaa gecgaggccc cttcgacgoc acaggaacct tccatcgtgc gaagagattc tggtgttcct gtgggcaccg ctgctgcgoc agatcgtgga aagggctact ctacgacgac ccgagctgga gagctgcccg agtgtctcoc gaagcgagct ttgatgagtt tttgtaacca gtgggaggtt goggagttag ctgoctgctg tggggagcct tgtatccgct cgtgtcgcoc tgctgaagat CCgaagaacg g
22. Caccgaggtt gcgccgtgct gtggacctga ctacttcaac acgacgatgg ctggagtggc gcccggcgcc ctcccaccaa gagctgcgga gagtttggac aaccattata aggtttttta ctcatgagac ccttattccc atcagttggg cgttttccaa CggtCgCCgC cagtaagaga CCEaaggagc agccatacca tacttactct cttccggctg agatggtaag ctgagatagg cttcattttt gttccactga tgcaaacaaa ggcttcagca accgoctaca actcaagacg acgacctaca gtatccggta ctgtcggett agcaacgcgg cagccatacc atgcaattgt aaagcatttt ccataccaca caattgttgt gcattttttt aatagcacca Cgagcgggag tggccctgaa ctgagcaact gcaggtggoc tgcgcttcgt Caccacgcct gaagttcttc agagccccct gccgtggccg gatcaccccc ccaccggcaa aacaagcagg acatttcttc tgctgctcca tgcatcgtgc gcgcatccgg agctgctggc aaaccacaac agctgcaata aagcaagtaa aataaccctg ttttttgcgg tgcacgagtg tgatgagcac atacactatt attatgcagt taaccgcttt aacgacgagc agcttcccgg gctggtttat ccctcccgta tgcctcactg aatttaaaag gcgtcagacc aaaaccaccg gagcgcagat tacctcgctc atagttaccg ccgaactgag agcggcaggg tcgccacctc cctttttacg acatttgtag tgttgttaac tttcactgca tttgtagagg tgttaacttg cactgcattc tcttcttcta ctgctggaga gaggaacgtg tcatggcccg cttattatgt ccactgcaag tcggaatgtt ctgagcacca ggtggaccag tggccaagcg cacgacgacg gtccctggga ccaccgagga gtggtggacc gcacccatcc tccaggaggg ggCggagtcg CCagaagaag tagaatgcag aacaagttaa aacctctaca ataaatgctt cattttgcct ggttacatcg ttttaaagtt ctcagaatga gctgccataa tttgcacaac gtgacaccac caacaattaa tgctgataaa tcgtagttat attaagcatt gatctaggtg ccgtagaaaa ctaccag
23. Eco 121 Eco O65I Eco T22 Fsel IPoo l Lsp 1 Mro1 Nae l Nrul NsiI Nsp V Pac Pf FI PAMI Pma CI Pmel Pmlil Psh Al Rsr Il Sap SbfI Sfil Sg71 Sna BI Sfl SGse8381 Stul Swal Tth 1111 Van 911 e ctcgaggtcg acggtatcga taagcttacg cgtgctagcg catgcagatc tactagtcgg ccggatatcg aattcctgca gcccaccacc atggctaacg agatcatcct gcacggcgcc aagcccaggg accccctgga cctgggcacc gccggcattc 161 agctctacag ggccctgacc aacttctcct tcctgaggga ggccctgatc gacgcccaca ccgaggaggt ggtgtcttac 241 gccgacatcc tggagaacag ctgtagactg gctaagtgct acgagaacta cggcctgcgc cagaacagcg tgatctccgt 321 gtgcagcgag aatagcacca tcttcttcta ccccgtgatc gccgccctgt acatgggcgt gatcaccgcc accgtgaacg 401 acagctacac cgagcgggag ctgctggaga ccctgaacat ctccaagccc gaactggtgt tctgctccaa gaaggccatc 481 aagaacatga tggccctgaa gaggaacgtg aacttcatca agaaggtggt gctgctggac agcaaggagg atatgggcga 561 ggcccagtgc ctgagcaact tcatggcccg gtactccgag cccaacctgg acgtgagaaa cttcaagcca agggacttcg 26 641 721 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3 61 3841 3921 4001 4081 4161 4241 4321 pSLO 1 81 161 241 321 401 acgccaagga aacctgagcg cgtgcccttc gattcgagga ttcctggcta Caccgaggtt gcgccgtgct gtggacctga ctacttcaac acgacgatgg ctggagtggc gcccggcgcc ctcccaccaa gagctgcgga Cacgctgccc agatgccatt tgaaaaaaat caacaacaat
24. Xhol 1 1 Bsp MI 2 1299 836 Not 1 1 1914 Xmal 1 2402 2 pSLG SV40 control pSLO SV40 control Aat II AccB7l Acc illl Afel Af Il Agel gr51H Asc Bae Bae I Ban Ill Bbr Pl Bbv Cl BA Bip 1 Boul102 BsiWI Bsm BI Bsp 1061 Bsp DI Bsp El Bss HII Bst 11071 Bst 981 Bst BI Bst Ell Bst PI szZ17I Cbil Clal Cpol Csp 451 Csp 1 Dra II Eco 1051 Eco47HI Eco 721 Eco O65 Fsel IPpo Lsp 1 Mro Nae Nrul Nsp V Pac Pf FI PH MI Pma CI Pme Pmlil Psh Al Rsrll Sall Sap SbfI Sg71 Sna BI Sfl Sse 83871 Swal Tth illl Van 911 pSLG SV40 control 1 ctcgagatct gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 81 tccgcccatt ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttacgcgtgc tagcgcatgc agatctacta 241 gtcggccgga tatcgaattc ctgcagccca ccaccatggc taacgagatc atcctgcacg gcgccaagcc cagggacccc 321 ctggacctgg gcaccgccgg cattcagctc tacagggccc tgaccaactt ctccttcctg agggaggccc tgatcgacgc 401 ccacaccgag gaggtggtgt cttacgccga catcctggag aacagctgta gactggctaa gtgctacgag aactacggcc 481 tgcgccagaa cagcgtgatc tccgtgtgca gcgagaatag caccatcttc ttctaccccg tgatcgccgc cctgtacatg 561 ggcgtgatca ccgccaccgt gaacgacagc tacaccgagc gggagctgct ggagaccctg aacatctcca agcccgaact 641 ggtgttctgc tccaagaagg ccatcaagaa catgatggcc ctgaagagga acgtgaactt catcaagaag gtggtgctgc 721 tgg
25. aactatggat actcatatat atgaccaaaa tecttttttt agctaccaac ttaggccacc tggcgataag gttcgtgcac acgcttcccg agggggaaac CaggggggCg atgttctttc cctccccctg aaagcaatag gtatcttatc CCCCCtgaac gcaatagcat tcttatcatg 30 ctccaagcca agaaggtgat catgcgttcg aacaaccggc acggcacccg gcttactttc caagatcgct ccagcctgac cacggcatcc ggececcatc agaagggcga gacaaggacg agaactcatc atgccggcgt atgaccgaga cgacagcatt ccaagcttag gggatggacc catgataaga atgctattgc gttcaggggg atcctcaaat ttcaacattt aaagtaaaag gagttttcgc acgccgggca catcttacgg tctgacaacg gggaaccgga aaactattaa accacttctg tcattgcagc gaacgaaata actttagatt tcccttaacg ctgcgcgtaa tctttttccg acttcaagaa tcgtgtctta acagcccagc aagggagaaa gcctggtatc gagcctatgg ctgcggtacc aacctgaaac catcacaaat atgtctggat ctgaaacata cacaaatttc tctg tgtctgatgt cgtgatcgac atccagtgaa ctgcctaagg catcgcccca cagtgggcct tctatcgtgg agagatcgcc tgcagggcta ggcaccccta gatttgcttc ggtggctgca aagtacaagg catcggcatc aggaggtgca ccgaaaggcc ccatggcttc gtcaccctgc tacattgatg tttatttgta aggtgtggga atgtatccgc CCgtgtcgcc atgctgaaga cccgaagaac agagcaactc atggcatgac atcggaggac gctgaatgaa ctggcgaact cgctcggccc actggggcca gacagatcgc gatttaaaac tgagttttcg tctgctgctt aaggtaactg ctctgtagca CCgggttgga ttggagcgaa ggcggacagg tttatagtcc aaaaacgcca gatcataatc ataaaatgaa ttcacaaata cataatcagc aaatgaatgc acaaataaag tctgttccaa agcatgtacg gttcaaccct gcgtggtgat gatacatcca gaagatcgtg tgcctcctcc tgtgg
26. aatgtggtat caataatatt tectgttttt aactggatct ctgctatgtg cttggttgag ccatgagtga atgggggatc gatgcctgta tagactggat tctggagccg ctacacgacg ggtaactgtc aagatccttt gatcaaagga tggtttgttt gttcttctag gttaccagtg agCggtcggg cgtgagctat agagcgcacg gtcgattttt ttttgctggc tgctttaaaa cagcttataa ggtttgtcca tttaaaaaac cttataatgg ttgtccaaac gcaggtggoc tgcgcttcgt Caccacgcct gaagttcttc agagccccct gccgtggccg gatcaccccc ccaccggcaa aacaagcagg acatttcttc tgctgctcca tgcatcgtgc gcgcatccgg agctgctggc atgtcttgtg cttctagagc gctttatttg tgcattcatt ggagctccog gaaaaaggaa gctcacccag caacagcggt gcgcggtatt tactcaccag taacactgcg atgtaactcg gcaatggcaa ggaggcggat gtgagcgtgg gggagtcagg agaccaagtt ttgataatct tcttcttgag gccggatcaa tgtagccgta gctgctgcca ctgaacgggg gagaaagcgc agggagcttc gtgatgctcg cttttgctca aacctcccac tggttacaaa aactcatcaa ctcccacacc ttacaaataa tcatcaatgt cttattatgt ccactgcaag tcggaatgtt ctgagcacca ggtggaccag tggccaagcg cacgacgacg gtccctggga ccaccgagga gtggtggacc gcacccatcc tccaggaggg ggCggagtcg CCagaagaag CCCaggagag ggCCgCCCag tgaaatttgt ttatgtttca CggCCCgggg gagtatgagt aaacgctggt aagatccttg atcccgtatt tcacagaaaa gccaacttac ccttgatcgt caacgttgcg aaagttgcag gtctcgcggt caactatgga tactcatata catgaccaaa atcctttttt gagctaccaa gttaggccac gtggcgataa ggttcgtgca cacgcttcoc Cagggggaaa tcaggggggc catgttcttt acctccccct taaagcaata tgtatcttat tccccctgaa agcaatagca atcttatcat PEST test
27. accaggtggc gacgaggccg ctacatcgcc ccggcaagct acatgataag gatgctattg ggttcagggg gatcctcaaa attcaacatt gaaagtaaaa agagttttcg gacgccgggc gcatcttacg ttctgacaac tgggaaccgg caaactatta gaccacttct atcattgcag tgaacgaaat tactttagat atcccttaac tctgcgcgta ctctttttcc cacttcaaga gtcgtgtctt cacagcccag gaagggagaa cgcctggtat ggagcctatg cctgcggtac gaacctgaaa gcatcacaaa catgtctgga cctgaaacat tcacaaattt gtctg ccggatatcg cctgggcacc ccgaggaggt cagaacagcg gaaggccatc atatgggcga agggacttcg gacccacagg tcctgtccat ctgctgaaga tgtgatggtg CCCctgtggg gagacctgct ggctaagatc tcatgaaggg ggatactacg ccccgccgag ccggcgagct gagcgagtgt ggtgagaagc atacattgat ctttatttgt gaggtgtggg tatgtatccg tccgtgtcgc gatgctgaag ccccgaagaa aagagcaact gatggcatga gatcggagga agctgaatga actggcgaac g cgctcggoc cactggggcc agacagatcg tgatttaaaa gtgagttttc atctgctgct gaaggtaact actctgtagc accgggttgg cttggagcga aggcggacag ctttatagtc gaaaaacgcc cgatcataat cataaaatga tttcacaaat tcataatcag aaaatgaatg cacaaataaa aattcctgca gccggcattc ggtgtcttac tgatctccgt 321 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3161 3841 3921 4001 4081 4161 gtgcagcgag acagctacac aagaacatga ggcccagtgc acgccaagga aacctgagcg cgtgcccttc gattcgagga ttcctggcta
28. gtcctgtcgg gccagcaacg aatcagccat tgaatgcaat aataaagcat cagccatacc atgcaattgt aaagcatttt gcgtgcgctt ttccaccacg ggagaagttc ctaagagccc gttgcogtgg gctgatcacc tgaccaccgg aacaacaagc tggacatttc ggctgctgct gcctgcatcg caagcgcatc ggaagctgct gacaaaccac ataagctgca ttaaagcaag gacaataacc cccttttttg gggtgcacga caatgatgag cgcatacact agaattatgc agctaaccgc ccaaacgacg tctagcttcc ctggctggtt aagccctccc aggtgcctca tttaatttaa tgagcgtcag aaaaaaacca gcagagcgca acatacctcg acgatagtta acaccgaact gtaagcggca gtttcgocac cggccttttt accacatttg tgttgttgtt ttttttcact acatttgtag tgttgttaac tttcactgca cgtccactgc ccttcggaat ttcctgagca cctggtggac ccgtggocaa CCCCaCgaCg caagtccctg aggccaccga ttcgtggtgg ccagcaccca tgctccagga cggegcggag ggcccagaag aactagaatg ataaacaagt taaaacctct ctgataaatg cggcattttg gtgggttaca cacttttaaa attctcagaa agtgctgcca ttttttgcac agcgtgacac cggcaacaat tattgctgat gtatcgtagt ctgattaagc aaggatctag accccgtaga ccgctaccag gataccaaat ctctgctaat ccggataagg gagataccta gggtcggaac ctctgacttg acggttcctg tagaggtttt aacttgttta gcattctagt aggttttact ttgtttattg ttctagttgt 6 pSLG PEST test pSLO PEST test 1 pSLG PEST test pSLO PEST test 1 2 Acc 651 1 3876 Bsp Cl 1 2600 Not 1 1859 Acc 1 2 7 263 Bsp DI 1 16 Nsp
29. tacagggccc 20 ttcgtccact cgccttcgga tcttcctgag CCCCtggtgg ggCCgtggCC ccccccacga ggcaagtccc gcaggccacc tcttcgtggt ctccagcacc cgtgctccag tccggggcgg ctggcccaga acaactagaa caataaacaa agtaaaacct ggcggagtta ggactttcca cacaccctaa taaccctgat ttttgcggca Cacgagtggg atgagcactt acactattct tatgcagtgc accgcttttt cgacgagcgt cttcccggca tggtttattg ctcccgtatc cctcactgat tttaaaagga gtcagacccc aaccaccgct gcgcagatac cctcgctctg agttaccgga gaactgagat Cggcagggtc gccacctctg tttttacggt atttgtagag ttgttaactt tcactgcatt tgtagaggtt ttaacttgtt ctgcattcta gcccctaact tttatgcaga tgcaaaaagc taacgagatc tgaccaactt gcaaggaccc atgttcacaa caccatcgag accagtacga aagcggctga cgacgtgaag tgggacctaa gaggaggcca ggaccggctg catccatcaa gagggcaaga agtcgtcttc agaagtccaa tgcagtgaaa gttaacaaca ctacaaatgt gegecgggac cacctggttg ctgacacaca aaatgcttca ttttgccttc ttacatcgaa ttaaagttct cagaatgact tgccataacc tgcacaacat gacaccacga acaattaata ctgataaatc gtagttatct taagcattgg tctaggtgaa gtagaaaaga accagcggtg caaatactgt ctaatcctgt taaggcgcag acctacagcg ggaacaggag acttgagcgt tcctggoctt gttttacttg gtttattgca ctagttgtgg ttacttgctt tattgcagct gttgtggttt ccgcccatcc geccgaggcc ttacgcgtgc atcctgcacg ctccttcctg cctgttcggc ccctgtocta aagtacagaa cctgtccagc agatcggcgg accggctcca caagagaggc tcgacaagga aaagagctga ggatgccggc gcctgaccga gtggacgaca gctgtaatct aaaatgcttt acaattgcat ggtagatctg tatggttgct ctgactaatt ttccaca
30. 1 2 40 3860 Ac l 2 2369 2742 Bsp MI 2 1114 651 Pae R7I 1 1 AF VI 2 28 3860 Bsr BRI 2 3882 4119 Pcil 1 3860 Ahd 1 2967 Bsr DI 2 2731 2913 Psh BI 1 2796 Alw 441 2 2300 3546 Bsr Gl 1 368 Psp 14061 2 2369 2742 Alw NI 2 565 3446 Bss SI 2 2303 3687 Psp Al 1 2104 Apal 1 170 Bst API 1 1810 Psp OMI 1 170 Apa LI 2 2300 3546 Bst XI 1 83 PstI 2 76 1808 Asel 1 2796 Bst ZI 2 58 1860 Pvul 1 2600 Aval 2 1 2104 Bsu 361 1 192 Pvu ll 1 258 Avill 1 2747 Cfr 91 1 2104 SacI 1 2092 Axy 1 1 192 Cla 1 16 Sac Il 1 2098 Ball 2 1016 1061 Dral 1 3752 Sall 1 7 Bam HI 1 2110 Eag 2 58 1860 San DI 1 128 Ban Ill 1 16 Fam 11051 1 2967 Scal 1 2489 Bbs 1 2 1152 1630 Ec HKI 1 2967 Sex Al 1 1421 Bbul 1 40 Eco 521 2 58 1860 Ser Al 2 1020 1509 Beg 1 2445 Eco 811 1 192 Smal 1 2104 Bell 1 2848 EcolCR 1 2092 Spel 1 52 Bel II 1 46 Eco RI 1 70 Sph I 1 40 Bip 1 1 1716 Eco RV 1 64 Ssp 1 1 2165 Bmr 1 2 2922 563 Fsp 1 2747 Tfi 2 881 923 Bou 1102 1 1716 Hd I 2 22 1714 Vsp I 1 2796 Bsa Al 1 1183 Kon 1 3876 Xbal 1 1853 Bsa BI 2 3882 4119 Mlu 1 1 28 Xho 1 1 Bso BI 2 1 2104 Msc 1 2 1016 1061 Xmal 1 2104 Bsp 1061 1 16 Ncol 2 89 1722 Bsp 14071 1 368 Nhe 1 34 2 pSLG PEST test pSLO PEST test Aat Aat Il 4ccB7I Acclll Afe Af il Age Aor 51HI Ascl Avr Il Bae Bor PI Bbv Cl Brrl Bin Bsi WI Bsm Bl Bsp El Bss HII Bst 11071 Bst 981 Bst Bl Bst Ell Bst PI srZ171 Cbil Cpo 1 Csp 451 Csp 1 Dra Ill Eco 105I co47ll
31. 10 04 MultiReporter Assay System Tripluc Vector Series Code No MRV 101 MRV 102 MRV 103 MRV 201 MRV 202 MRV 203 MRV 301 MRV 501 MRV 502 MRV 503 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3556K MultiReporter Assay System Tripluc 1 2 short life 3 1 aaa S S M ia 2 3 Le
32. 16 Kon 1 3755 Xba 1 1732 Bsr Bl 2 1736 2007 Lsp 1 250 c 7 1 2 968 1307 Bsr DI 2 2610 2792 Milu 1 28 XhoI 1 1 Bsr GI 1 404 Mly 2 3369 2852 Xmal 1 1983 Bss Sl 2 2182 3566 Nar 1 1162 Xmn 1 1 224 Bst BI 1 250 Ncol 1 89 2 pSLR test Aati Aat Il 4ccB7I Acclill Afe Af Il Age or51HI Apal Asc 1 Avr Il Bae I Bbr Pl Brrl Bin 1 Bip Boui Bsa Al Bsi WI Bsm Bl Bsp El Bss HII Bst 11071 Bst 981 Bst API srZ171 Btrl Coo 1 Csp Dra Ill Eco 1051 Eco47HI Eco 721 EcoO109 EcoT22I Fsel IPpo l Mro1 Nae Nrul NsiI Pac PfI FI PH MI Pma CI Pme Pmlil Ppu MI Psh AI Psp OMI Rsr Il San DI Sap I Sfil Sna BI Srfl Stul Swal Tth 1111 Van 911 pSLR test 1 ctcgaggtcg acggtatcga taagcttacg cgtgctagcg catgcagatc tactagtcgg ccggatatcg aattcctgca 91 gcccaccacc atggaagaag agaacatcgt gaatggcgat cgccctcggg atctggtgtt ccctggcaca gccggcctgc 161 agctgtatca gtccctgtat aaatactctt acatcaccga cggaatcatc gacgcccaca ccaacgaggt gatctcctat 241 gcccagattt tcgaaacaag ttgccgcctg gccgtgagcc tggagaagta tggcctggat cacaacaacg tggtggccat 17 321 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2002 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 ttgcagcgag atatgtacac ccattcatcc cgtggagtgc atccoctgga agca
33. 187 Alw NI 2 778 3529 Bst ZI 2 271 1943 Psp OMI 1 383 Apal 1 383 Bsu 361 1 405 PstI 2 217 289 Apa LI 2 2383 3629 Cbil 1 127 Psh BI 1 2879 Asel 1 2879 Cfr 9 i 2187 Psp 14061 2 2452 2825 Aval 2 1 2187 Csp 451 1 127 Pvul 1 2683 Avill 1 2830 Dral 1 3835 Pvu Il 1 411 Axy1 1 405 gg 1 2 271 1943 Sac 1 2175 Ball 2 1229 1274 Eam1105l 1 3050 Sac Il 1 2181 Bam HI i 2193 c HKI 1 3050 San DI 1 341 6s 2 1365 1843 Eco 521 2 271 1943 Scal 1 2572 Bbul 1 253 Eco 811 1 405 Sex AI 1 1634 Bcgl i 2528 Eco ICRI 1 2175 Sar AI 2 1233 1722 Ball 1 2931 Eco RI 2 124 283 Smal 1 2187 Bel II 1 259 Eco RV 1 277 Spe 1 265 Bmr 2 3005 716 Fspl 1 2830 Sph 1 1 253 Bsa Al 1 1396 Hind Ill 1 235 Ssp I 1 2248 Bsa BI 2 3965 4202 o 1 3959 Tfil 2 1094 1136 Bso BI 2 1 2187 Lsp 1 1 127 Vsp 1 1 2879 Bsp 14071 1 581 Milu 1 2 188 241 Xbal 1 1936 Bsp CI i 2683 Msc 2 1229 1274 Xhol 1 1 Bsp MI 2 1327 864 Ncol 1 302 Xmal 1 2187 Bsr BRI 2 3965 4202 Nhel 1 241 2 pSLG HSVtk Aat Aat Il MccB7I Acclll Afel Af II Agel M4or51HI Asci Avr Il Bae Bael Ban Ill Bor PI Bbv CI Bfri Bin 1 Bip 1 Boul102 Bs WI Bsm Bl Bsp 1061 Bsp DI Bsp El Bss HII Bst 11071 Bst 981 Bst API Bst Ell Bst PI BstZl7l Cial Cpo Csp 1 Dra II Eco 1051 co47III Eco 721 Eco O68l Eco T22I Fsel IPpo Mro1 Nae Nrul NsiI Pac PFI Pfi MI Pma CI Pme Pmlil Psh Al Rsrll Sall Sap SbfI Sfil Sg71 Sna BI Sfl SGse
34. 8381 Stul Swal Tthi111 Van 911 pau HSVtk ctcgagctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta i gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg goggcgcggt 161 cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgaccogc ttaaaagctt 241 acgcgtgcta gcgcatgcag atctactagt cggccggata tcgaattcct gcagcccacc accatggcta acgagatcat 321 cctgcacggc gccaagccca gggaccccct ggacctgggc accgccggca ttcagctcta cagggccctg accaacttct 401 ccttcctgag ggaggccctg atcgacgccc acaccgagga ggtggtgtct tacgccgaca tcctggagaa cagctgtaga 481 ctggctaagt gctacgagaa ctacggcctg cgccagaaca gcgtgatctc cgtgtgcagc gagaatagca ccatcttctt 561 ctaccccgtg atcgccgccc tgtacatggg cgtgatcacc gccaccgtga acgacagcta caccgagcgg gagctgctgg 641 agaccctgaa catctccaag cccgaactgg tgttctgctc caagaaggcc atcaagaaca tgatggccct gaagaggaac 721 gtgaacttca tcaagaaggt ggtgctgctg gacagcaagg aggatatggg cgaggcccag tgcctgagca acttcatggc 801 ccggtactcc gagcccaacc tggacgtgag aaacttcaag ccaagggact tcgacgccaa ggagcaggtg gcccttatta 24 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 4401 tgtcctcctc aaggaccccc gttcacaacc ccatcgagaa cagtacgacc gcggctgaag acgtgaagac ggacctaaca ggaggccatc accggctgaa tccatcaagg g
35. Al 1 2113 Bam HI 1 2119 Drdl 1 3761 Pvul 2 117 2609 Ban Ill 1 16 Eagl 2 58 1869 Pvu Il 2 160 1519 Bbel 1 1162 Eam1104l 2 97 2187 Sac 1 2101 Bbs 1 1 903 Fam 11051 2 1070 2976 Sac Il 1 2107 Bbul 1 40 Earl 2 97 2187 Sall 1 7 Bbv CI 1 1686 c HKI 2 1070 2976 Sbfl 1 1089 BciMI 2 2133 3660 Eco 521 2 58 1869 Scal 1 2498 Bel 2 662 707 Eco 811 1 694 Sex Al 1 1424 Bgll 2 1658 2857 EcolCRi 1 2101 Sfol 1 1162 Bel Ml 1 46 Eco O65l 1 1599 Sefl 1 116 Bio 1 1 1725 Eco RI 1 70 Sar Al 1 1341 Bmrl 1 2931 Eco RV 1 64 Smal 1 2113 Bou 11021 1 1725 EcoT14 2 89 1731 Spel 1 52 Bse RI 2 1753 944 Ehel 1 1162 Sph 1 1 40 Bsp 1061 1 16 Foal 2 662 707 Sse 83871 1 1089 Bsp 14071 1 404 Fsp 1 1 2756 Ssp 1 2174 Bsp Cl 2 117 2609 Hrd 2 22 1723 Sty 2 89 1731 Bsp DI 1 16 Kas 1 1162 Vsp 1 1 2805 Bsp MI 1 1616 Kpnl 1 3885 Xbal 1 1862 Bsr DI 2 2740 2922 Lsp 1 250 c 7 1 2 968 1307 Bsr Gl 1 404 Milu 1 28 Xho 1 1 Bss SI 2 2312 3696 2 3499 2982 Xmal 1 2113 Bst API 1 1819 Nar 1 1162 777 1 1 2377 2 pSLR PEST test Aat Aat Il 4ccB7I Acclll Afe Af il Age Aor 51HI Apal Asc Avr Il Bael Bor PI Brrl Bin Bsa Al Bsi WI Bsm BI Bsp El Bss HII Bst 11071 Bst 981 sr Z171 Btrl Coo 1 Csp Dra II Eco 1051 co41 Eco 721 EcoO109 EcoT22I Fsel IPpo l Mro 1 Nae l Nrul NsiI Pac PAFI PA MI Pma CI Pme Pmlil Ppu MI Psh AI Psp OMI Rsr II San DI Sap Sfil Sna BI Srf1 Stul Swal Tth 1111 Van 911 p
36. Bsal 2 3207 1296 Eco T22l 2 2251 2323 Sef 1 301 Bse RI 2 172 1129 Ehel 1 1347 Sgr Al 1 1526 Bso 14071 1 589 Fbal 2 847 892 Smal 1 2411 Bsp Cl 2 302 2907 Fspl 1 3054 Spel 1 237 Bsp MI 1 1801 Hind Ill 1 207 Sse 83871 1 1274 Bsr DI 2 3038 3220 Kas 1 1347 Ssp 1 1 2472 Bsr Gl 1 589 Kon 1 4183 Stul 1 188 Bss SI 2 2610 3994 Lspl 1 435 Styl 2 191 274 Bst API 2 2253 2325 Mlu 1 2 213 787 Vsp 1 3103 Bst BI 1 435 1 2 3797 3280 Xba 1 1917 Bst Ell 1 1784 Nar 1 1 1347 Nem 1 2 1153 1492 Bst Pl 1 1784 co 1 1 274 Xho 1 1 Bst KI 1 268 Nhe 1 219 Xmal 1 2411 Bst Zl 2 243 1924 Not 1 1923 Xmn 1 1 2675 2 pSLR SV40 control Aat Il AccB7l Acci Acc II Afe Af Il Age Moro1HI Apa Asc 1 Bae I Ban Ill Bor PI BF Bip 1 Boul102 Bsa Al Bsi WI Bsm Bl Bsp 1061 Bsp DI Bsp EI Bss HII Bst 11071 Bst 981 sr Z17I Btrl Clal Coo 1 Cs I Dra II Eco 1051 Eco47HI Eco 721 coO109I FseI IPoo l Mro 1 Nae Nrul Pac Pfi FI Pfi MI Pma CI Pme 77 1 Ppu MI Psh AI Psp OMI Asril Sall San DI Sap I Sna BI Srfl Swal Tth1111 Van9il pSLR SV40 control 1 ctcgagatct gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 81 tccgcccatt ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttacgcgtgc tagcgcatgc agatctacta 241 gtcggccgga tatcgaattc ctgcagccca ccaccatgga agaagagaac atcgtgaat
37. G Filter 3 SLO Filter 1 Tio SLO Filter 1 Filter 2 T20 SLO Filter 2 Filter 3 T3o SLO Filter 3 SLR Filter 1 3538 3 Tir SLR Filter 1 Filter 2 T2r SLR Filter 2 Filter 3 T3r SLR Filter 3 3 SLG SLO SLR G O R Filter 1 F1 Filter 2 F2 Filter 3 F3 F1 Tig Tio Tir G F2 T2g T2o T2r O F3 T3g T3o T3r R F0 F1 F2 G O R G Tig Tio Tir 1 T ri O T2g T2o T2r F2 R T3g T3o T3r F3 Microsoft Excel Microsoft Excel 3 x3 MININVERSE
38. G PEST test PEST 1720 1842 PSLOIPEST test SV40 late poly A signal 1860 2090 4355 bp ISLO B lactamase Amp r gene 2185 3045 background reduction signal 3876 4355 SLGOR F primer 4098 4122 SLGO R primer complementary 105 128 Not 1859 Sspl 2165 Sac 1 2092 BamH 12110 N Sac II 2098 Smal 2104 12 7 pSLR PEST test Kpn 13885 pSLR PEST test Multiple cloning region 1 77 SLR gene 91 1728 PEST 1729 1851 SV40 late poly A signal 1869 2099 pSLR PEST test 4364 bp p lactamase Amp r gene 2194 3054 background reduction signal 3885 4364 SLGOR F primer 4107 4131 SLR R primer complementary 108 130 Not 1868 as 2174 Sac 2101 BamH 2119 Sac l 2107 Sma 2113 13 6 1 pSLG test pSLO test 1 pSLG test pSLO test 1 2 Acc 651 1 3746 Bsp CI 1 2410 Ncol 1 89 Acc 2 7 263 Bsp DI 1 16 Nhe 1 34 Ac l 2 2239 2612 Bsp MI 2 1114 651 NotI 1 1729 AH III 2 28 3730 Bsr BRI 2 3752 3989 Nsp 2 40 3730 Ahd1 1 2837 Bsr DI 2 2601 2783 Pae R7I 1 1 Alw 441 2 2170 3416 Bsr QI 1 368 Poil 1 3730 Alw NI 2 565 3316 Bss SI 2 2173 3557 Psh BI 1 2666 Apal 1 170 Bst KI 1 83 Psp Al 1 1974 Apa LI 2 2170 3416 Bst Z 2 58 1730 PstI 1 76 Asel 1 2666 Bsu 361 1 192 Pvul 1 2470 Aval 2 1 1974 C
39. SLR PEST test 1 ctcgaggtcg acggtatcga taagcttacg cgtgctagcg catgcagatc tactagtcgg ccggatatcg aattcctgca 81 gcccaccacc atggaagaag agaacatcgt gaatggcgat cgccctcggg atctggtgtt ccctggcaca gccggcctgc 161 agctgtatca gtccctgtat aaatactctt acatcaccga cggaatcatc gacgcccaca ccaacgaggt gatctcctat 241 gcccagattt tcgaaacaag ttgccgcctg gccgtgagcc tggagaagta tggcctggat cacaacaacg tggtggccat 321 ttgcagcgag aacaacatcc acttcttcgg ccctctgatc gctgccctat accaggggat tccaatggcc acatccaacg 29 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 atatgtacac ccattcatcc cgtggagtgc atcccctgga agcatcacca cgcccctttc agtttgaggg tatctggcca cagagacatc gcgccctgat gtgattgaca gggctaccac acgacgagga gagctggaga gctgccttcc tgaccaccac aacgagctga ggctgctggc tcaatgtgta agaatgcagt acaagttaac acctctacaa taaatgcttc attttgcoctt gttacatcga tttaaagttc tcagaatgac ctgccataac ttgcacaaca tgacaccacg aacaattaat gctgataaat cgtagttatc ttaagcattg atctaggtga cgtagaaaag taccagcggt ccaaatactg gctaatcctg ataaggcgca tacctacagc cggaacagga gacttgagcg ttcctggcct ggttttactt tgtttattgc tctagttgtg tttacttgct ttattgcagc agttgtggtt Cgagagggag tgaaggtgca gtgttcagtt gagaaccgcg tcagattcgt caccacgcct cgagttcttc aga
40. a tgtatccgct tcaacatttc cgtgtcgcoc aagtaaaaga tgctgaagat agttttcgcc ccgaagaacg cgccgggcaa gagcaactcg atcttacgga tggcatgaca ctgacaacga tcggaggacc ggaaccggag ctgaatgaag aactattaac tggcgaacta ccacttctgc gctcggccct cattgcagca ctggggccag aacgaaatag acagatcgct ctttagattg atttaaaact cccttaacgt gagttttcgt tgcgcgtaat ctgctgcttg ctttttccga aggtaactgg cttcaagaac tctgtagcac cgtgtcttac cgggttggac cagcccagct tggagcgaac agggagaaag gcggacaggt cctggtatct ttatagtcct agcctatgga aaaacgccag tgcggtaccg atcataatca acctgaaaca taaaatgaat atcacaaatt tcacaaataa tgtctggatc ataatcagcc tgaaacataa aatgaatgca acaaatttca caaataaagc ctg pSLO SV40 control 1 81 161 241 321 ctcgagatct tccgcccatt ccagaagtag gtcggccgga ctggacctgg gcatctcaat tagtcagcaa ctccgcccca tcgctgacta tgaggagect tttttggagg tatcgaattc ctgcagccca gcaccgccgg cattcagctc gagcgtgcgc ccttccacca gaggagaagt ggctaagagc aggttgccgt gtgctgatca cctgaccacc tcaacaacaa gatggacatt gtggctgctg gcgcctgcat accaagcgca goggaagctg tggacaaacc ttataagctg ttttaaagca gggcgggatg gggagoctgg ggggactttc catgagacaa ttattcoctt cagttgggtg ttttccaatg gtcgccgcat gtaagagaat gaaggagcta ccataccaaa cttactctag tccggctggc atggtaagcc gagataggtg tcatttttaa tccactgagc caaacaaaaa cttcagcaga cgcctacata tcaagacgat gacctacacc atccggtaag gtcgggtttc caacgcggcc gccataccac gcaattgttg agcatttttt ataccacatt attgttgttg atttttttca ccatagtccc atttttttta cctaggcttt ccaccatggc
41. acag ctttatagtc gaaaaacgcc cgatcataat cataaaatga tttcacaaat tcataatcag aaaatgaatg cacaaataaa 2 PSLR test 1 pSLR test 1 2 Acc 651 1 3755 Bst Ell 1 1599 Nhe 1 1 34 Acc 1 7 Bst PI 1 1599 Not 1 1738 Ac I 2 2248 2621 Bst XI i 83 Nsp V 1 250 Aff Ill 2 28 3739 Bst ZI 2 58 1739 Pae R7I 1 1 Ahd 2 1070 2846 Bsu 361 1 694 Pcil 1 3739 Alw 441 2 2179 3425 Cbil 1 250 Ple l 2 2852 3369 Apa LI 2 2179 3425 Cfr 9 i 1983 Psh BI 1 2675 Asel 1 2675 Clal 1 16 Psp 14061 2 2248 2621 Avill 1 2626 Csp 451 1 250 Psp Al 1 1983 AxyI 1 694 Dral 1 3631 Pvul 2 117 2479 Bam HI 1 1989 Eagl 2 58 1739 Pvu Il 2 160 1519 Ban Ill 1 16 Eam1104l 2 97 2057 SacI 1 1971 Bbel 1 1162 Eam1105I 2 1070 2846 Sac Il 1 1977 6s 1 903 Earl 2 97 2057 Sall 1 7 Bbul i 40 c HKI 2 1070 2846 Sbf1 1 1089 Bbv CI 1 1686 Eco 521 2 58 1739 Scal 1 2368 Bci VI 2 2003 3530 Eco 811 1 694 Sex AI 1 1424 Bel 2 662 707 Eco ICRI 1 1971 Sfol 1 1162 Bell 2 1658 2727 Eco O65l 1 1599 Sefl 1 116 Bel Il 1 46 Eco RI 1 70 Sar Al 1 1341 Bmrl 1 2801 Eco RV 1 64 Smal 1 1983 Bsal 2 2779 1111 Eco T14I 1 89 Spel 1 52 Bse RI 1 944 Ehel 1 1162 Sph 1 1 40 Bsp 1061 1 16 Fbal 2 662 707 Sse 83871 1 1089 Bsp 14071 1 404 Fspl i 2626 Ssp I 1 2044 Bsp CI 2 117 2479 Hind Ill 1 22 Styl 1 89 Bsp DI 1 16 Kas 1 1 1162 Vsp 1 2675 Bsp MI 1 16
42. acagcaa ggaggatatg ggcgaggccc agtgcctgag caacttcatg gcccggtact ccgagcccaa cctggacgtg 801 agaaacttca agccaaggga cttcgacgcc aaggagcagg tggcccttat tatgtcctcc tctggcacca ccggcctgcc 19 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 4401 4481 4561 4641 aaagggcgtg tcccctccac ggoctgagag cgtgctggcc tggccaccgg ggctacggcc agccccctac tcaagagcga cactccggcg ggectaccag tgcccgacga atcgactaca CgCCaCCggC cccagacatg tttgtgatgc tttcaggttc cggagaatgg gatgcatgct ctttgcatac gcccggggga gtatgagtat acgctggtga gatccttgag cccgtattga acagaaaagc caacttactt ttgatcgttg acgttgcgca agttgcagga ctcgcggtat actatggatg ctcatatata tgaccaaaat cctttttttc gctaccaact taggccacca ggcgataagt ttcgtgcaca cgcttcccga gggggaa4Cg aggggggcgg tgttctttcc ctccccctga aagcaatagc tatcttatca cccctgaacc caatagcatc cttatcatgt gtgctgaccc acaggaacct ctccatcctg tccatcgtgc tggtgctgct gaagagattc cctcctgtga tggtgttcct cggcgcccct gtgggcaccg tgaccgagac ctgctgcgcc gtgcaggcta agatcgtgga gatcatcatg aagggctact acgtgggata ctacgacgac gtggcccccg ccgagctgga ggccgccggc gagctgcccg tcgccgagcg agtgtctccc aagctggtga gaagcgagct ataagataca ttgatgagtt tattgcttta tttgtaacca agggggaggt gtgggaggtt gcggaactgg gcggagttag ttgcatactt ctgcctgctg ttctgcctgc tggggagcct tcctcaaat
43. ag gcgtcagacc aaaaccaccg gagcgcagat tacctcgctc atagttaccg ccgaactgag agcggcaggg tcgccacctc cctttttacg acatttgtag tgttgttaac tttcactgca tttgtagagg tgttaacttg cactgcattc pSLO test 1 81 161 241 ctcgaggtcg gcccaccacc agctctacag gccgacatcc acggtatcga atggctaacg ggccctgacc tggagaacag ctgctggaga gaggaacgtg tcatggcccg cttattatgt ccactgcaag tcggaatgtt ctgagcacca ggtggaccag tggccaagcg cacgacgacg gtccctggga ccaccgagga gtggtggacc gcacccatcc tccaggaggg ggCggagtcg CCagaagaag tagaatgcag aacaagttaa aacctctaca ataaatgctt cattttgcct ggttacatcg ttttaaagtt ctcagaatga gctgccataa tttgcacaac gtgacaccac caacaattaa tgctgataaa tcgtagttat attaagcatt gatctaggtg ccgtagaaaa ctaccagcgg accaaatact tgctaatcct gataaggcgc atacctacag tcggaacagg tgacttgagc gttcctggoc aggttttact ttgtttattg ttctagttgt ttttacttgc tttattgcag tagttgtggt taagcttacg agatcatcct aacttctcct ctgtagactg ccctgaacat aacttcatca gtactccgag cctcctctgg gaccccctgt cacaaccctg tcgagaagta tacgacctgt gctgaagatc tgaagaccgg cctaacaaga ggccatcgac ggctgaaaga atcaaggatg caagagcctg tcttcgtgga tccaagctgt tgaaaaaaat caacaacaat aatgtggtat caataatatt tectgttttt aactggatct ctgctatgtg cttggttgag ccatgagtga atgggggatc gatgcctgta tagactggat tctggagccg ctacacgacg ggtaactgtc aagatccttt gatcaaagga tggtttgttt gttcttctag gttaccagtg agCggtcggg cgtgagctat agagcgcacg gtcgattttt ttttgctggc tgctttaaaa cagcttataa ggttt
44. agcaactcg tggcatgaca tcggaggacc ctgaatgaag tggcgaacta gctcggccct ctggggccag acagatcgct atttaaaact gagttttcgt ctgctgcttg aggtaactgg tctgtagcac cgggttggac tggagcgaac gcggacaggt ttatagtcct aaaacgccag atcataatca taaaatgaat tcacaaataa ataatcagcc aatgaatgca caaataaagc catcctggag gcgagaatag tacaccgagc catgatggcc agtgcctgag aaggagcagg gagcgtgcgc ccttccacca gaggagaagt ggctaagagc aggttgccgt gtgctgatca cctgaccacc tcaacaacaa gatggacatt gtggctgctg gcgcctgcat accaagcgca gcggaagctg tggacaaacc ttataagctg ttttaaagca gggcgggatg gggagcctgg ggggactttc catgagacaa ttattccctt cagttgggtg ttttccaatg gtcgcogcat gtaagagaat gaaggagcta ccataccaaa cttactctag tccggctggc atggtaagcc gagataggtg tcatttttaa tccactgagc caaacaaaaa cttcagcaga cgcctacata tcaagacgat gacctacacc atccggtaag gtcgggtttc caacgcggcc gccataccac gcaattgttg agcatttttt ataccacatt attgttgttg atttttttca 21 aacagctgta caccatcttc gggagctgct ctgaagagga caacttcatg tggcccttat ttcgtccact cgccttcgga tcttcctgag CCCCtggtgg ggCCgtggcc ccccccacga ggcaagtccc gcaggccacc tcttcgtggt ctccagcacc cgtgctccag tccggggcgg ctggcccaga acaactagaa caataaacaa agtaaaacct ggcggagtta ggactttcca cacaccctaa taaccctgat ttttgcggca cacgagtggg atgagcactt acactattct tatgcagtgc accgcttttt cgacgagcgt cttcccggca tggtttattg ctcccgtatc cctcactgat tttaaaagga gtcagacccc aaccaccgct gcgcagatac cctcgctctg agttaccgga gaactgagat cggcagggtc gccacctctg tttttacggt att
45. c ctctgatgcc agaccatgac ttcatcgaca caagtccaag gcagtgaaaa ttaacaacaa tacaaatgtg gggCgggact acctggttgc tgacacacat aatgcttcaa tttgccttcc tacatcgaac taaagttctg agaatgactt gccataacca gcacaacatg acaccacgat caattaatag tgataaatct tagttatcta aagcattggt ctaggtgaag tagaaaagat CCagcggtgg aaatactgtt taatcctgtt aaggcgcagc cctacagcgt gaacaggaga cttgagcgtc cctggccttt ttttacttgc tttattgcag tagttgtggt tacttgcttt attgcagctt ttgtggtttg gtgatcgtga gttcgatcca Ccggcctgcc acccgcatcg ctttccagtg tcgcttctat ctgacagaga catcctgcag ccatcggcac ggcgagattt ggaCcgggtgg tcatcaagta ggcgtcatcg cgagaaggag gcattccgaa ctgtaatcta aaatgcttta caattgcatt gtagatctga atggttgctg tgactaattg tccacagcga taatattgaa tgtttttgct tggatctcaa ctatgtggcg ggttgagtac tgagtgataa ggggatcatg gcctgtagca actggatgga ggagccggtg Cacgacgggg aactgtcaga atcctttttg caaaggatct tttgtttgcc cttctagtgt accagtggct ggtcgggctg gagctatgag gcgcacgagg gatttttgtg tgctggcctt tttaaaaaac cttataatgg ttgtccaaac aaaaaacctc ataatggtta tccaaactca 5 PSLG HSVtk control 1 pSLG HSVtk 1 2 Acc 651 1 3959 Bsr DI 2 2814 2996 Not 1 1942 Acc 1 476 Bsr GI 1 581 Nsp V 1 127 Ac l 2 2452 2825 Bss SI 2 2386 3770 Pae R7I 1 1 Ahdl 1 3050 Bst BI 1 127 Poil 1 3943 Alw 441 2 2383 3629 Bst XI 1 296 Psp AI 1 2
46. c ctgtagcacc gggttggact ggagcgaacg cggacaggta tatagtcctg aaacgccagc tcataatcag aaaatgaatg cacaaataaa taatcagcca atgaatgcaa aaataaagca 23 gtgcagaagc cagtttcgtg ccgcgctgat ttcgtccaca cgccttcgga tcttcctgaa cctctggtgg caaggtggcc gccccaacga accggcaaag CCCaCaggCC tcatctatgt cttctgcagc tgtcgtgctg acctgcgggg atctttgccc ggacaaacca tataagctgc tttaaagcaa ggCgggatgg ggagcctggg gggactttcc atgagacaat tattcccttt agttgggtgc tttccaatga tcgccgcata taagagaatt aaggagctaa cataccaaac ttactctagc ccggctggct tggtaagccc agataggtgc catttttaat ccactgagcg aaacaaaaaa ttcagcagag gcctacatac caagacgata acctacaccg tccggtaagc tcgggtttcg aacgcggcct ccataccaca caattgttgt gcattttttt taccacattt ttgttgttgt tttttttcac acctggactt tcccggtaca catgacatcc gcagcgatcc ctgtttaccg gaccatccag atgagtacaa aagagactga tagagagctg ccctgggacc accagggatg ggtggatcgg accctaacat gagcctggca cggcgtggtg gcgagcaggc caactagaat aataaacaag gtaaaacctc gCggagttag gactttccac acaccctaac aaccctgata tttgcggcat acgagtgggt tgagcacttt cactattctc atgcagtgct ccgctttttt gacgagcgtg ttcccggcaa ggtttattgc tcccgtatcg ctcactgatt ttaaaaggat tcagaccccg accaccgcta cgcagatacc ctcgctctgc gttaccggat aactgagata ggCagggtcg ccacctctga ttttacggtt tttgtagagg tgttaacttg cactgcattc gtagaggttt taacttgttt tgcattctag tctcaagaag ccgatcatgc tctggaacaa catctacggc ccctggctta aactacaaga tctgtccagc aggtccacgg aagaagggcg aagagagaag ccctggacaa ctgaaagaa
47. caagccc ctgccaaagg aaccatcccc tcgtgggcct acaatcgtgc agaggtggcc tccagggcta agggtagccc gtgcttcaag ggctgcactc tacaagggct cggcgtgccc agatcatcga aagggcgcca CCCECCEEag cagcctgtgc gagtttggac aaccattata aggtttttta ctcatgagac ccttattccc atcagttggg cgttttocaa Cggtcgocgc cagtaagaga CCgaaggagc agccatacca tacttactct cttccggctg agatggtaag ctgagatagg cttcattttt gttccactga tgcaaacaaa ggcttcagca accgcctaca actcaagacg acgacctaca gtatccggta ctgtcgggtt agcaacgcgg cagccatacc atgcaattgt aaagcatttt ccataccaca caattgttgt gcattttttt tactagtcgg accccctgga gacgcccaca Cggcctgcgc acatgggcgt gaactggtgt gcgtggtgct tccacctcca gagagtggtg tggcccctcc accggcggcg cggcctgacc cctacgtgca agcgagatca CggCgacgtg accaggtggc gacgaggccg ctacatcgcc ccggcaagct gtggaggagc ttctgctagg aaaccacaac agctgcaata aagcaagtaa aataaccctg ttttttgcgg tgcacgagtg tgatgagcac atacactatt attatgcagt taaccgcttt aacgacgagc agcttcccgg gctggtttat ccctccogta tgcctcactg aatttaaaag gcgtcagacc aaaaccaccg gagcgcagat tacctcgctc atagttaccg ccgaactgag agcggcaggg tcgccacctc cctttttacg acatttgtag tgttgttaac tttcactgca tttgtagagg tgttaacttg cactgcattc ccggatatcg cctgggcacc ccgaggaggt cagaacagcg gatcaccgcc tctgctccaa gacccacagg tcctgtccat ctgctgaaga tgtgatggtg CCCctgtggg gagacctgct ggctaagatc tcatgaaggg ggatactacg ccccgccgag ccggcgagct gagcgagtgt ggtgagaagc aggctgctgg atcaatgtgt tagaatgcag aacaagttaa aacctctaca ataaatgctt cattt
48. catgtctgga cctgaaacat tcacaaattt gtctg 28 agaaggtggt cccaacctgg caccaccggc tcggcaacag tcctacttca cagaatccca ccagcatcag ggcggcatcc ctccaccggc gaggcgagct aaggagggct gctgatcaag ccggcgtgac accgagcagg cgacatcccc gccatggctt cgtcaccctg atacattgat ctttatttgt gaggtgtggg tatgtatccg tccgtgtcgc gatgctgaag ccccgaagaa aagagcaact gatggcatga gatcggagga agctgaatga actggcgaac gcgctcggcc cactggggcc agacagatcg tgatttaaaa gtgagttttc atctgctgct gaaggtaact actctgtagc accgggttgg cttggagcga aggcggacag ctttatagtc gaaaaacgcc cgatcataat cataaaatga tttcacaaat tcataatcag aaaatgaatg cacaaataaa gctgctggac acgtgagaaa ctgccaaagg aaccatcccc tcgtgggcct acaatcgtgc agaggtggcc tccagggcta agggtagccc gtgcttcaag ggctgcactc tacaagggct Cggcgtgccc agatcatcga aagggcgcca cccgcoggag cagcctgtgc gagtttggac aaccattata aggtttttta ctcatgagac ccttattccc atcagttggg cgttttccaa cggtcgccge cagtaagaga ccgaaggagc agccatacca tacttactct cttccggctg agatggtaag ctgagatagg cttcattttt gttccactga tgcaaacaaa ggcttcagca accgcctaca actcaagacg acgacctaca gtatccggta ctgtcgggtt agcaacgcgg cagccatacc atgcaattgt aaagcatttt ccataccaca caattgttgt gcattttttt agcaaggagg cttcaagcca gcgtggtgct tccacctcca gagagtggtg tggcccctcc accggcggcg cggcctgacc cctacgtgca agcgagatca cggcgacgtg accaggtggc gacgaggccg ctacatcgcc ccggcaagct gtggaggagc ttctgctagg aaaccacaac agctgcaata aagcaagtaa aataaccctg ttttttgcgg tgcacgagtg tgatgagcac ataca
49. ccga ggcaagctga ccagacatga ttgtgatgct ttcaggttca ggagaatggg atgcatgctt tttgcatact cccgggggat tatgagtatt cgctggtgaa atccttgaga ccgtattgac cagaaaagca aacttacttc tgatcgttgg cgttgcgcaa gttgcaggac tcgcggtatc ctatggatga tcatatatac gaccaaaatc ctttttttct ctaccaactc aggccaccac gcgataagtc tcgtgcacac gcttcccgaa Egggaaacgc ggggggCgg4 gttctttcct tccccctgaa agcaatagca atcttatcat ccctgaacct aatagcatca ttatcatgtc ccctgccatt aacggcgtgg gtttgatccc acaggagcat gccatcgccc gaaaaagttt tggtgtatct ctgggcagag ctgtagcgoc tgaaggtgat atgaagggct ctactacgac ctgccgagct ggccagctgc gctggtgacc tgagaaacga taagatacat attgctttat gggggaggtg Cggaactggg tgcatacttc tctgoctgct cctcaaatat caacatttcc agtaaaagat gttttcgcoc gccgggcaag tcttacggat tgacaacgat gaaccggagc actattaact cacttctgcg attgcagcac acgaaataga tttagattga ccttaacgtg gcgcgtaatc tttttccgaa ttcaagaact gtgtcttacc agcccagctt ggagaaagg ctggtatctt gcctatggaa gcggtaccga cctgaaacat tcacaaattt gtctggatca gaaacataaa caaatttcac tg catcctgaag agtgcgtgtt ctggagagaa caccatcaga ctttccacca gagggcgagt ggccaagagc acatcgccga ctgatcctga tgacatcaac accacaacaa gaggacagat ggagaacttg cttccgcctg accaccaagc gctgagggcc tgatgagttt ttgtaaccat tgggaggttt cggagttagg tgcctgctgg ggggagcctg gtatccgctc gtgtcgccct gctgaagatc cgaagaacgt agcaactcgg ggcatgacag cggaggaccg tgaatgaagc ggcgaactac ctcggccctt tggggccaga cagatcgctg tttaaaactt agttttcgtt tgctgcttgc ggtaactgg
50. cg acttcaagaa tcgtgtctta acagcccagc aagggagaaa gcctggtatc gagcctatgg ctgcggtacc aacctgaaac catcacaaat atgtctggat ctgaaacata cacaaatttc tctg 3 PSLG SV40 control pSLO SV40 control 1 pSLG SV40 control pSLO SV40 control 1 5 Aat I 1 188 Bsr DI 2 3029 3211 NsiI 2 2242 2314 Acc 651 1 4174 Bsr Gl 1 553 Pae RTI 1 1 Acc 1 448 Bss Sl 2 2601 3985 Pcil 1 4158 Ac l 2 2667 3040 Bst API 2 2244 2316 Psh BI 1 3094 AH I 2 213 4158 Bst XI 1 268 Psp 14061 2 2667 3040 Ahdl i 3265 Bst Z 2 243 1915 Psp Al 1 2402 Alw 441 2 2598 3844 Bsu 361 1 377 Psp OMI 1 355 Alw NI 2 750 3744 Cfr 9 1 2402 PstI 1 261 Apal 1 355 Dral 1 4050 Pvul 1 2898 Apa LI 2 2598 3844 Eagl 2 243 1915 Pvu Il 1 443 Asel 1 3094 Earn105I 1 3265 SacI 1 2390 Aval 2 1 2402 c HKI 1 3265 Sac Il 1 2396 Avill 1 3045 Eco 521 2 243 1915 San DI 1 313 Avr II 1 191 Eco 811 1 371 Scal 1 2787 AxyI 1 377 Eco ICRI 1 2390 Sex AI 2 1606 2292 Ball 2 1201 1246 Eco RI i 255 Sfil 1 137 Bam HI 1 2408 Eco RV 1 249 Sar Al 2 1205 1694 Bbs 2 1337 1815 EcoT22 2 2242 2314 Smal 1 2402 Beg 1 2743 Fsp 1 1 3045 Spel 1 237 Ball 2 138 3146 Hind Ill 1 207 Ssp 1 2463 Bin 1 1 191 KpnI 1 4174 Stul 1 188 Bsa Al 1 1368 Milu 1 1 213 Tfil 2 1066 1108 Bso BI 2 1 2402 MscI 2 1201 1246 Vsp I 1 3094 Bsp 14071 1 553 Ncol 1 274 Xbal 1 1908 Bsp CI 1 2898 Nhel 1 219
51. cgg accaaatact tgctaatcct gataaggcgc atacctacag tcggaacagg tgacttgagc gttcctggcc aggttttact ttgtttattg ttctagttgt ttttacttgc tttattgcag tagttgtggt ccccgtgatc ccctgaacat aacttcatca gtactccgag cctcctctgg gaccccctgt cacaaccctg tcgagaagta tacgacctgt gctgaagatc tgaagaccgg cctaacaaga ggccatcgac ggctgaaaga atcaaggatg caagagcctg tcttcgtgga tccaagctgt tgaaaaaaat caacaacaat aatgtggtat caataatatt tectgttttt aactggatct ctgctatgtg cttggttgag ccatgagtga atgggggatc gatgcctgta tagactggat tctggagccg ctacacgacg ggtaactgtc aagatccttt gatcaaagga tggtttgttt gttcttctag gttaccagtg agcggtcggg cgtgagctat agagcgcacg gtcgattttt ttttgctggc tgctttaaaa cagcttataa ggtttgtcca tttaaaaaac cttataatgg ttgtccaaac 16 gccgcoctgt ctccaagccc agaaggtggt cccaacctgg caccaccggc tcggcaacag tcctacttca cagaatccca ccagcatcag ggcggcatcc ctccaccggc gaggcgagct aaggagggct gctgatcaag ccggcgtgac accgagcagg cgacatcccc aatctagagc gctttatttg tgcattcatt ggagctcccg gaaaaaggaa gctcacccag caacagcggt gcgcggtatt tactcaccag taacactgcg atgtaactcg gcaatggcaa ggaggcggat gtgagcgtgg gggagtcagg agaccaagtt ttgataatct tcttcttgag gccggatcaa tgtagccgta gctgctgcca Ctgaacgggg gagaaagcgc agggagcttc gtgatgctcg cttttgctca aacctcccac tggttacaaa aactcatcaa ctcccacacc ttacaaataa tcatcaatgt acatgggcgt gaactggtgt gctgctggac acgtgagaaa ctgccaaagg aaccatcccc tcgtgggcct acaatcgtgc agaggtggoc tccagggcta agggtagccc gtgcttcaag ggctgca
52. cggct tggcctgacc tgccctatgt aagagccaga caccggcgat gctaccaggt ccagacgagt ggattatatc caacaggcaa CCECCEEagg agcctgtgct agtttggaca accattataa ggttttttaa tcatgagaca cttattccct tcagttgggt gttttccaat ggtcgcogca agtaagagaa cgaaggagct gccataccaa acttactcta ttccggctgg gatggtaagc tgagataggt ttcattttta ttccactgag gcaaacaaaa gcttcagcag ccgcctacat ctcaagacga cgacctacac tatccggtaa tgtcgggttt Caacgcggc agccatacca tgcaattgtt aagcattttt cataccacat aattgttgtt catttttttc gaagtccctg acatcaacgg aaagagtttg cagccacagg tcctggccat atggtgaaaa aatcatggtg CCCctctggg gagacctgta CCaggtgaag tgctgatgaa ctgggctact ggcccctgcc ttgccggcca gccgagctgg gctgatgaga tggaggagca tctgctagga aaccacaact gctgcaataa agcaagtaaa ataaccctga tttttgcggc gcacgagtgg gatgagcact tacactattc ttatgcagtg aaccgctttt acgacgagcg gcttcccggc ctggtttatt cctcccgtat gcctcactga atttaaaagg cgtcagaccc aaaccaccgc agcgcagata acctcgctct tagttaccgg cgaactgaga gCggCagggt cgccacctct ctttttacgg catttgtaga gttgttaact ttcactgcat ttgtagaggt gttaacttgt actgcattct 7 1 PCR GC PCR PCR
53. ctatt attatgcagt taaccgcttt aacgacgagc agcttcccgg gctggtttat ccctcccgta tgcctcactg aatttaaaag gcgtcagacc aaaaccaccg gagcgcagat tacctcgctc atagttaccg ccgaactgag agcggcaggg tcgccacctc cctttttacg acatttgtag tgttgttaac tttcactgca tttgtagagg tgttaacttg cactgcattc atatgggcga agggacttcg gacccacagg tcctgtccat ctgctgaaga tgtgatggtg CCCctgtggg gagacctgct ggctaagatc tcatgaaggg ggatactacg ccccgccgag ccggcgagct gagcgagtgt ggtgagaagc aggctgctgg atcaatgtgt tagaatgcag aacaagttaa aacctctaca ataaatgctt cattttgcct ggttacatcg ttttaaagtt ctcagaatga gctgccataa tttgcacaac gtgacaccac caacaattaa tgctgataaa tcgtagttat attaagcatt gatctaggtg ccgtagaaaa ctaccagcgg accaaatact tgctaatcct gataaggcgc atacctacag tcggaacagg tgacttgagc gttcctggcc aggttttact ttgtttattg ttctagttgt ttttacttgc tttattgcag tagttgtggt 7 pSLR PEST test 1 pSLR PEST test 1 2 Acc 651 1 3885 Bst BI i 250 Ncol 2 89 1731 Acc 1 7 Bst Ell 1 1599 Nhe 1 34 Ac l 2 2378 2751 Bst PI 1 1599 Notl 1 1868 Afi Ill 2 28 3869 Bst XI i 83 Nsp V 1 250 Ahdi 2 1070 2976 Bst Z 2 58 1869 Pae RII 1 1 Alw 441 2 2309 3555 Bsu 361 1 694 Pcil 1 3869 Apa LI 2 2309 3555 Cbil 1 250 Plel 2 292 3499 Asel 1 2805 Cfr 9 1 2113 Psh BI 1 2805 Avill 1 2756 Cg 1 16 Psp 14061 2 2378 2751 Axyl 1 694 Csp 451 1 250 Psp
54. ctc tacaagggct Cggcgtgccc agatcatcga aagggcgcca ggccgcccag tgaaatttgt ttatgtttca CggCCCgggg gagtatgagt aaacgctggt aagatccttg atcccgtatt tcacagaaaa gccaacttac ccttgatcgt caacgttgcg aaagttgcag gtctcgoggt caactatgga tactcatata catgaccaaa atcctttttt gagctaccaa gttaggccac gtggcgataa ggttcgtgca Cacgcttccc cagggggaaa tcaggggggc catgttcttt acctccccct taaagcaata tgtatcttat tccccctgaa agcaatagca atcttatcat gatcaccgoc tctgctocaa agcaaggagg cttcaagcca gcgtggtgct tccacctcca gagagtggtg tggcccctcc accggcggcg cggcctgacc cctacgtgca agcgagatca cggcgacgtg accaggtggc gacgaggccg ctacatcgcc ccggcaagct acatgataag gatgctattg ggttcagggg gatcctcaaa attcaacatt gaaagtaaaa agagttttcg gaCgCCgggC gcatcttacg ttctgacaac tgggaaccgg caaactatta gaccacttct atcattgcag tgaacgaaat tactttagat atcccttaac tctgcgcgta ctctttttcc cacttcaaga gtcgtgtctt cacagcccag gaagggagaa cgcctggtat ggagcctatg cctgcggtac gaacctgaaa gcatcacaaa catgtctgga cctgaaacat tcacaaattt gtctg accgtgaacg gaaggccatc atatgggcga agggacttcg gacccacagg tcctgtccat ctgctgaaga tgtgatggtg CCCctgtggg gagacctgct ggctaagatc tcatgaaggg ggatactacg ccccgccgag ccggcgagct gagcgagtgt ggtgagaagc atacattgat ctttatttgt gaggtgtggg tatgtatccg tccgtgtcgc gatgctgaag ccccgaagaa aagagcaact gatggcatga gatcggagga agctgaatga actggcgaac g cgctcggoc cactggggcc agacagatcg tgatttaaaa gtgagttttc atctgctgct gaaggtaact actctgtagc accgggttgg cttggagcga aggcgg
55. fr 9 1 1974 Pvu Il 1 258 Avill 1 2617 Cg 1 1 16 Sac 1 1962 Axy1 1 192 Dral 1 3622 Sac Il 1 1968 Ball 2 1016 1061 Eagl 2 58 1730 Sall 1 7 Bam HI 1 1980 Earn1105I 1 2837 San DI 1 128 Ban lI 1 16 Ec HKI 1 2837 Scal 1 2359 Bbs 1 2 1152 1630 Eco 521 2 58 1730 Sex AI 1 1421 Bbul i 40 Eco 811 i 192 Sar Al 2 1020 1509 Bcgl i 2315 Eco ICRI 1 1962 Sma 1 1 1974 Bell 1 2718 Eco RI 1 70 Spe 1 52 Bel M 1 46 Eco RV 1 64 Sph 1 1 40 Bmr 2 2192 563 Fspl 1 2617 Ssp I 1 2035 Bsa Al 1 1183 Hind Ill 1 22 Tfil 2 881 923 Bsa BI 2 3752 3989 Psp OMI 1 170 Vsp I 1 2666 Bso BI 2 1 1974 Kon 1 3746 Xba 1 1723 Bsp 1061 1 16 Milu 1 28 XhoI 1 1 Bsp 14071 1 368 MscI 2 1016 1061 Xmal 1 1974 2 pSLG test pSLO test Aati Aat Il 4ccB7I Acclll Afel Af II Agel Aor 5H AscI Av I Bael Bbr Pl Bbv Cl Brrl Bin 1 Bip Boull102 Bsi WI Bsm Bl Bsp El Bss HII Bst i070 Bst 981 Bst API Bst BI Bst EII Bst PI szZ171 Cbil Cpol Csp 451 Csp 1 Dra Ill Eco 1051 Fco4Ml Eco 121 Eco O65 EcoT22I Fsel IPpo Lsp 1 Mro 1 Nae Nrul NsiI Nsp V Pac Pf FI PAMI Pma CI Pme Pmlil Psh Al Rsrll Sap SbfI Sfil Sg71 Sna BI Srfl Sse8387 Stul Swal Tth 1111 Van 911 pSLG test 1 ctcgaggtcg acggtatcga taagcttacg cgtgctagcg 81 gcccaccacc atggctaacg agatcatcct gcacggcgcc 161 agctctacag ggccctgacc aacttctcct tcctgaggga 241 gccgacatcc tggagaacag ctgtagactg gctaagtgct 321 gtgcagcgag aatagcacca tcttcttcta ccccgt
56. g gcgatcgccc tcgggatctg 321 gtgttccctg gcacagccgg cctgcagctg tatcagtccc tgtataaata ctcttacatc accgacggaa tcatcgacgc 401 ccacaccaac gaggtgatct cctatgccca gattttcgaa acaagttgcc gcctggccgt gagcctggag aagtatggcc 481 tggatcacaa caacgtggtg gccatttgca gcgagaacaa catccacttc ttcggccctc tgatcgctgc cctataccag 561 gggattccaa tggccacatc caacgatatg tacaccgaga gggagatgat cggccacctg aacatctcca agccatgtct 22 641 721 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 4401 4481 4561 4641 gatgttctgt tcgacagcat gtgaagttca taagggcgtg CCCCagatac ggcctgaaga cgtggtgcct tcgcctgtgg ggctatggcc ccctatgccc gcttcaagag ctgcacaccg caagggctac gcatcccaga gtgcaggatt aggcccaaca gagCggCCgC tttgtgaaat cattttatgt acgatggagc actaattgag agatgcatgc gctcccgcgg aaaggaagag cacccagaaa cagcggtaag cggtattatc tcaccagtca cactgcggcc taactcgcct atggcaacaa ggcggataaa agcgtgggtc agtcaggcaa ccaagtttac ataatctcat tcttgagatc ggatcaagag agccgtagtt gctgccagtg aacggggggt aaagcgccac gagcttccag atgctcgtca ttgctcacat ctcccacacc ttacaaataa tcatcaatgt ccacacctcc caaataaagc tcaatgtatc tccaagaagt gtacgacatc accctaaaga gtgatcagcc atccatcctg tcgtgatggt cctccaatca cggctcccct tgaccgagac tatgtccagg ccagatgctg gcgatctggg caggtggccc cgagtttgcc atatcg
57. gatc 14 catgcagatc tactagtcgg ccggatatcg aattcctgca aagcccaggg accccctgga cctgggcacc gccggcattc ggccctgatc gacgcccaca ccgaggaggt ggtgtcttac acgagaacta cggcctgcgc Cagaacagcg tgatctccgt gccgccctgt acatgggcgt gatcaccgcc accgtgaacg 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3161 3841 3921 4001 4081 4161 acagctacac aagaacatga ggcccagtgc acgccaagga aacctgagcg cgtgcccttc gattcgagga ttcctggcta Caccgaggtt gcgccgtgct gtggacctga ctacttcaac acgacgatgg ctggagtggc gcccggcgcc ctcccaccaa gagctgcgga gagtttggac aaccattata aggtttttta ctcatgagac ccttattccc atcagttggg cgttttccaa CggtCgCCgC cagtaagaga CCEaaggagc agccatacca tacttactct cttccggctg agatggtaag ctgagatagg cttcattttt gttccactga tgcaaacaaa ggcttcagca accgoctaca actcaagacg acgacctaca gtatccggta ctgtcggett agcaacgcgg cagccatacc atgcaattgt aaagcatttt ccataccaca caattgttgt gcattttttt Cgagcgggag tggccctgaa ctgagcaact gcaggtggoc tgcgcttogt caccacgcct gaagttcttc agagcccoct gccgtggocg gatcacccoc ccaccggcaa aacaagcagg acatttcttc tgctgctcca tgcatcgtgc gcgcatccgg agctgctggc aaaccacaac agctgcaata aagcaagtaa aataaccctg ttttttgcgg tgcacgagtg tgatgagcac atacactatt attatgcagt taaccgcttt aacgacgagc agcttcccgg gctggtttat ccctcccgta tgcctcactg aatttaaa
58. gccctct gccgacaagg cctgagccoc tcaacaccgg aacaacccac cagattcatc acttgcttct gCctgtgtcg caagcacctg gggccatctt acgctgccca gatgccattc gaaaaaaatg aacaacaatt atgtggtatg aataatattg cctgtttttg actggatctc tgctatgtgg ttggttgagt catgagtgat tgggggatca atgcctgtag agactggatg ctggagccgg tacacgacgg gtaactgtca agatcctttt atcaaaggat ggtttgtttg ttcttctagt ttaccagtgg gcggtcgggo gtgagctatg gagcgcacga tcgatttttg tttgctggcc gctttaaaaa agcttataat gtttgtccaa ttaaaaaacc ttataatggt tgtccaaact atgatcggcc gaagcacctg tcgtgtcccg ctgatcatga ccacagcagc tcggactgtt ctgaagacca ggtggatgag tggccaagag aacgatagag caaagccctg aggccaccag tatgtggtgg gcagcaccct tgctggagoc CggggCggCg tgcccgcgag tgtcttgtgc ttctagagcg ctttatttgt gcattcattt gagctcccgc aaaaaggaag ctcacccaga aacagcggta cgcggtatta actcaccagt aacactgcgg tgtaactcgc caatggcaac gaggCggata tgagcgtggg ggagtcaggc gaccaagttt tgataatctc cttcttgaga ccggatcaag gtagccgtag ctgctgccag tgaacggggg agaaagcgcc gggagcttoc tgatgctcgt ttttgctcac acctcccaca ggttacaaat actcatcaat tcccacacct tacaaataaa catcaatgta acctgaacat gactttctca gtacaccgat catcctctgg gatcccatct taccgccctg tccagaacta tacaatctgt actgaaggtc agctgaagaa ggaccaagag ggatgccctg atcggctgaa aacatctctg tggcaagacc tggtgttcat caggccaagt ccaggagagc gccgcccaga gaaatttgtg tatgtttcag ggCCCggggg agtatgagta aacgctggtg agatccttga tcccgtattg cacagaaaag ccaacttact cttgatcgtt aacgttgcgc aagttgcagg tctcgcggta
59. gcctcactga atttaaaagg cgtcagaccc aaaccaccgc agcgcagata acctcgctct tagttaccgg cgaactgaga gCggCagggt cgccacctct ctttttacgg catttgtaga gttgttaact ttcactgcat ttgtagaggt gttaacttgt actgcattct ccctctgatc acctgaacat gactttctca gtacaccgat catcctctgg gatcccatct taccgccctg tccagaacta tacaatctgt actgaaggtc agctgaagaa ggaccaagag ggatgccctg atcggctgaa aacatctctg tggcaagacc tggtgttcat caggccaagt agaatgcagt acaagttaac acctctacaa taaatgcttc attttgcctt gttacatcga tttaaagttc tcagaatgac ctgccataac ttgcacaaca tgacaccacg aacaattaat gctgataaat cgtagttatc ttaagcattg atctaggtga cgtagaaaag taccagcggt ccaaatactg gctaatcctg ataaggcgca tacctacagc cggaacagga gacttgagcg ttcctggcct ggttttactt tgtttattgc tctagttgtg tttacttgct ttattgcagc agttgtggtt 18 gctgcoctat ctccaagcca agaaggtgat catgcgttcg aacaaccggc acggcacccg gcttactttc caagatcgct ccagcctgac cacggcatcc gggCgCCatc agaagggcga gacaaggacg agaactcatc atgccggcgt atgaccgaga cgacagcatt ccaagctgta gaaaaaaatg aacaacaatt atgtggtatg aataatattg cctgtttttg actggatctc tgctatgtgg ttggttgagt catgagtgat tgggggatca atgcctgtag agactggatg ctggagccgg tacacgacgg gtaactgtca agatcctttt atcaaaggat ggtttgtttg ttcttctagt ttaccagtgg gCggtcgggc gtgagctatg gagcgcacga tcgatttttg tttgctggcc gctttaaaaa agcttataat gtttgtccaa ttaaaaaacc ttataatggt tgtccaaact accaggggat tgtctgatgt cgtgatcgac atccagtgaa ctgcctaagg catcgcocca cagtgggcct tctatcgtgg agagatcgcc
60. gcg ataatattga ctgtttttgc ctggatctca gctatgtggc tggttgagta atgagtgata gggggatcat tgcctgtagc gactggatgg tggagcoggt acacgacggg taactgtcag gatccttttt tcaaaggatc gtttgtttgc tcttctagtg taccagtggc cggtcgggct tgagctatga agcgcacgag cgatttttgt ttgctggcct ctttaaaaaa gcttataatg tttgtccaaa taaaaaacct tataatggtt gtccaaactc cgcccctaac gcctcggoct tagcgcatgc gCgCCaagCC agggaggccc accagaacca cttcatcgtg tcccaacaat atcagagagg catcctccag CCggCagggt gagctgtgct gggctggctg tcaagtacaa gtgaccggcg gcaggagatc tccccaaggg agagcggccg atttgtgaaa tcattttatg aacgatggag gactaattga gagatgcatg agctcccgcg aaaaggaaga tcacccagaa acagcggtaa gcggtattat ctcaccagtc acactgcggc gtaactcgcc aatggcaaca aggcggataa gagcgtgggt gagtcaggca accaagttta gataatctca ttcttgagat cggatcaaga tagccgtagt tgctgccagt gaaCgggggg gaaagcgcca ggagcttcca gatgctcgtc tttgctcaca cctcccacac gttacaaata ctcatcaatg cccacacctc acaaataaag atcaatgtat tccgcccagt ctgagctatt agatctacta cagggacccc tgatcgacgc 401 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 4401 4481 4561 4641 ccacaccgag tgcgccagaa ggcgtgatca ggtgttctgc tggacagcaa agaaacttca aaagggcgtg tcccctccac ggcctgagag cgtgctggcc tggccaccgg ggctacggcc
61. ggcaagagc tcgtcttcgt aagtccaagc cagtgaaaaa taacaacaac acaaatgtgg cttcaataat ccttoctgtt tcgaactgga gttctgctat tgacttggtt taaccatgag aacatggggg cacgatgcct taatagactg aaatctggag tatctacacg attggtaact gtgaagatcc aaagatcaaa cggtggtttg actgttcttc cctgttacca cgcagcggtc cagcgtgagc aggagagcgc agcgtcgatt gocttttgct acttgcttta ttgcagctta tgtggtttgt tgctttaaaa cagcttataa ggtttgtcca tggcaccacc tgttcggcac ctgtcctact gtacagaatc tgtccagcat atcggcggca cggctccacc agagaggcga gacaaggagg agagctgatc atgccggcgt ctgaccgagc ggacgacatc tgtaatctag aatgctttat aattgcattc tatggagctc attgaaaaag tttgctcacc tctcaacagc gtggcgcggt gagtactcac tgataacact atcatgtaac gtagcaatgg gatggaggCg CCggtgagCg acggggagtc gtcagaccaa tttttgataa ggatcttctt tttgccggat tagtgtagcc gtggctgctg gggctgaacg tatgagaaag acgagggagc tttgtgatgc ggccttttgc aaaaacctcc taatggttac ccaaactcat aacctcccac tggttacaaa aactcatcaa ggCCtgccaa cagaaccatc tcatcgtggg ccaacaatcg cagagagetg tcctccaggg ggCagggtag gctgtgcttc gctggctgca aagtacaagg gaccggcgtg aggagatcat cccaagggcg agcggccgcc ttgtgaaatt attttatgtt CCgCggCCCg gaagagtatg cagaaacgct ggtaagatcc attatcccgt cagtcacaga gcggccaact tcgocttgat caacaacgtt gataaagttg tgggtctcgc aggcaactat gtttactcat tctcatgacc gagatccttt caagagctac gtagttaggc ccagtggcga gggggttcgt Cgccacgctt ttccaggggg tcgtcagggg tcacatgttc cacacctccc aaataaagca caatgtatct acctccccct taaagcaata tgtatcttat agggcgt
62. ggt ccctccacot cctgagagtg tgotggcocc gccaccggcg ctacggcctg cccoctacgt aagagcgaga ctcoggcgac gctaccaggt cccgacgagg cgactacatc ccaccggcaa cagacatgat tgtgatgcta tcaggttcag ggggatcctc agtattcaac ggtgaaagta ttgagagttt attgacgccg aaagcatctt tacttctgac cgttgggaac gcgcaaacta caggaccact ggtatcattg ggatgaacga atatacttta aaaatccctt ttttctgcgc Caactctttt caccacttca taagtcgtgt gcacacagcc CCCEaaggga aaacgcctgg ggCggagcct tttcctgcgg cctgaacctg atagcatcac tatcatgtct gaacctgaaa gcatcacaaa catgtctg 25 gctgacccac ccatcctgtc gtgctgctga tcctgtgatg gcgococtgt accgagacct gcaggctaag tcatcatgaa gtgggatact ggcccccgcc ccgccggcga gccgagcgag gctggtgaga aagatacatt ttgctttatt ggggaggtgt aaatatgtat atttccgtgt aaagatgctg tcgccccgaa ggcaagagca acggatggca aacgatcgga cggagctgaa ttaactggcg tctgcgctcg Cagcactggg aatagacaga gattgattta aacgtgagtt gtaatctgct tccgaaggta agaactctgt cttaccgggt cagcttggag gaaaggcgga tatctttata atggaaaaac taccgatcat aaacataaaa aaatttcaca ggatcataat cataaaatga tttcacaaat aggaacctga catcgtgccc agagattcga gtgttcctgg gggcaccgag gctgcgocgt atcgtggacc gggctacttc acgacgacga gagctggagt gctgcccggc tgtctcccac agcgagctgc gatgagtttg tgtaaccatt gggaggtttt ccgctcatga cgcccttatt aagatcagtt gaacgttttc actcggtcgc tgacagtaag Egaccgaagg tgaagccata aactacttac gcccttccgg gccagatggt tcgctgagat aaacttcatt ttcgttocac gcttgcaaac actggcttca agcaccgcct tggactcaag cgaacgacct caggtatccg
63. glll Spel EcoRV EcoRI stecapeoca ccaccatgg Pst SLG SLO SLR 1 Hind pSLG SLO SLR PEST test 2 Pst 1 SLR pSLR test pSLR PEST test SV40 poly A signal PCR pSLG pSLO SLGO R primer Code No MRV 402 Xhol Sall Clal Hind Miul Nhel Sphl Bglll Spel EcoRV EcoRI Pstl 90 100 110 120 130 140 150 160 gcccaccacc atggctaacg agatcatcct gcacggcgcc aagcccaggg accccctgga cctgggcacc gccggcattc SLG SLO L GTAGGA CGTGCCGCGG TTCGGGTC 170 180 agctctacag ggccctgacc SLGO R primer pSLG test pSLO test pSLG PEST test pSLO PEST test 1 Hind pSLG PEST SLO PEST test
64. gtcca tttaaaaaac cttataatgg ttgtccaaac cgtgctagcg gcacggcgcc tcctgaggga gctaagtgct 15 ctccaagccc agaaggtggt cccaacctgg caccaccggc tcggcaccag tcctacttca cagaatccca ccagcatcag ggcggcatcc ctccaccggc gaggcgagct aaggagggct gctgatcaag ccggcgtgac accgagcagg cgacatcccc aatctagagc gctttatttg tgcattcatt ggagctcccg gaaaaaggaa gctcacccag caacagcggt gcgcggtatt tactcaccag taacactgcg atgtaactcg gcaatggcaa ggaggcggat gtgagcgtgg gggagtcagg agaccaagtt ttgataatct tcttcttgag gccggatcaa tgtagccgta gctgctgcca ctgaacgggg gagaaagcgc agggagcttc gtgatgctcg cttttgctca aacctcccac tggttacaaa aactcatcaa ctcccacacc ttacaaataa tcatcaatgt catgcagatc aagcccaggg ggccctgatc acgagaacta gaactggtgt gctgctggac acgtgagaaa ctgccaaagg aaccatcccc tcgtgggcct acaatcgtgc agaggtggcc tccagggcta agggtagccc gtgcttcaag ggctgcactc tacaagggct Cggcgtgccc agatcatcga aagggcgcca ggccgcccag tgaaatttgt ttatgtttca CggCCCgggg gagtatgagt aaacgctggt aagatccttg atcccgtatt tcacagaaaa gccaacttac ccttgatcgt caacgttgcg aaagttgcag gtctcgoggt caactatgga tactcatata catgaccaaa atcctttttt gagctaccaa gttaggccac gtggcgataa ggttcgtgca Cacgcttccc cagggggaaa tcaggggggc catgttcttt acctccccct taaagcaata tgtatcttat tccccctgaa agcaatagca atcttatcat tactagtcgg accccctgga gacgcccaca cggcctgcge tctgctocaa agcaaggagg cttcaagcca gcgtggtgct tccacctcca gagagtggtg tggcccctcc accggcggcg cggcctgacc cctacgtgca agcgagatca CggCgacgtg
65. t gggctggctg tcaagtacaa gtgacCggCg gCaggagatc tccccaaggg agagcggccg atttgtgaaa tcattttatg aacgatggag gactaattga gagatgcatg agctcccgog aaaaggaaga tcacccagaa acagcggtaa gcggtattat ctcaccagtc acactgcggc gtaactcgoc aatggcaaca aggcggataa gagcgtgggt gagtcaggca accaagttta gataatctca ttcttgagat cggatcaaga tagccgtagt tgctgccagt gaaCgggggg gaaagcgcca ggagcttcca gatgctcgtc tttgctcaca cctcccacac gttacaaata ctcatcaatg cccacacctc acaaataaag atcaatgtat 4 PSLR SV40 control 1 pSLR SV40 control 1 2 Aat 1 188 Bsu 361 1 879 NsiI 2 2251 2323 Acc 651 1 4183 Csp 451 1 435 Nsp V 1 435 Ac I 2 2676 3049 Cbil 1 435 Pae RTI 1 1 AhdI 2 1255 3274 Cfr 91 1 2411 Pcil 1 4167 Alw 441 2 2607 3853 Dral 1 4059 Plel 2 3280 3797 Apa LI 2 2607 3853 Eag 2 243 1924 Psh BI 1 3103 Asel 1 3103 Eam1104l 2 282 2485 Psp 14061 2 2676 3049 Avill 1 3054 Fam 11051 2 1255 3274 Psp Al 1 2411 Avr Il 1 191 Earl 2 282 2485 Pvul 2 302 2907 Axy l 1 879 Ec HKI 2 1255 3274 Pvu ll 2 345 1704 Bam HI 1 2417 Eco 521 2 243 1924 Sac 1 2399 Bbe 1 1347 Eco 811 1 879 Sac Il 1 2405 Bobs 1 1 1088 Eco ICRI 1 2399 Sbfl 1 1274 Bbv CI 1 1871 Eco O65 1 1784 Scal 1 2796 Bci VI 2 2431 3958 Eco RI 1 255 Sex Al 2 1609 2301 Bell 2 847 892 Eco RV 1 249 Sfil 1 137 Bini 1 191 Eco T14I 2 191 274 Sfol 1 1347
66. tatttg tgcattcatt ggagctccog gaaaaaggaa gctcacccag caacagcggt gcgcggtatt tactcaccag taacactgcg atgtaactcg gcaatggcaa ggaggcggat gtgagcgtgg gggagtcagg agaccaagtt ttgataatct tcttcttgag gccggatcaa tgtagccgta gctgctgcca ctgaacgggg gagaaagcgc agggagcttc gtgatgctcg cttttgctca aacctcccac tggttacaaa aactcatcaa ctcccacacc ttacaaataa tcatcaatgt gaggaacgtg tcatggcccg cttattatgt ccactgcaag tcggaatgtt ctgagcacca ggtggaccag tggccaagcg cacgacgacg gtccctggga ccaccgagga gtggtggacc gcacccatcc tccaggaggg ggCggagtcg CCagaagaag CCCaggagag geccgcccag tgaaatttgt ttatgtttca CggCCCgggg gagtatgagt aaacgctggt aagatccttg atcccgtatt tcacagaaaa gccaacttac ccttgatcgt caacgttgcg aaagttgcag gtctcgcggt caactatgga tactcatata catgaccaaa atcctttttt gagctaccaa gttaggccac gtggcgataa ggttcgtgca cacgcttcoc Cagggggaaa tcaggggggc catgttcttt acctccccct taaagcaata tgtatcttat tccccctgaa agcaatagca atcttatcat aacttcatca gtactccgag cctcctctgg gaccccctgt cacaaccctg tcgagaagta tacgacctgt gctgaagatc tgaagaccgg cctaacaaga ggccatcgac ggctgaaaga atcaaggatg caagagcctg tcttcgtgga tccaagctta cgggatggac acatgataag gatgctattg ggttcagggg gatcctcaaa attcaacatt gaaagtaaaa agagttttcg gaCgCCgggC gcatcttacg ttctgacaac tgggaaccgg caaactatta gaccacttct atcattgcag tgaacgaaat tactttagat atcccttaac tctgcgcgta ctctttttcc cacttcaaga gtcgtgtctt cacagcccag gaagggagaa cgcctggtat ggagcctatg cctgcggtac gaacctgaaa gcatcacaaa
67. tcacca cgccoctttc agtttgaggg tatctggcca cagagacatc gcgcoctgat gtgattgaca gggctaccac acgacgagga gagctggaga gctgccttcc tgaccaccac aacgagctga tacattgatg tttatttgta aggtgtggga atgtatccgc CCgtgtcgcc atgctgaaga cccgaagaac agagcaactc atggcatgac atcggaggac gctgaatgaa ctggcgaact Cgctcggccc actggggcca gacagatcgc gatttaaaac tgagttttcg tctgctgctt aaggtaactg ctctgtagca ccgggttgga ttggagcgaa ggcggacagg tttatagtcc aaaaacgcca gatcataatc ataaaatgaa ttcacaaata cataatcagc aaatgaatgc acaaataaag aacaacatcc cgagagggag tgaaggtgca gtgttcagtt gagaaccgcg tcagattcgt caccacgcct cgagttcttc agagccctct gccgacaagg cctgagccoc tcaacaccgg aacaacccac cagattcatc acttgcttct gcctgtgtcg caagcacctg ggeccatctt agtttggaca accattataa ggttttttaa tcatgagaca cttattccct tcagttgggt gttttccaat ggtcgccgca agtaagagaa cgaaggagct gccataccaa acttactcta ttccggctgg gatggtaagc tgagataggt ttcattttta ttccactgag gcaaacaaaa gcttcagcag ccgcctacat ctcaagacga cgacctacac tatccggtaa tgtcgggttt gCaaCgCggC agccatacca tgcaattgtt aagcattttt cataccacat aattgttgtt catttttttc acttcttcgg atgatcggcc gaagcacctg tcgtgtcccg ctgatcatga ccacagcagc tcggactgtt ctgaagacca ggtggatgag tggccaagag aacgatagag caaagccctg aggccaccag tatgtggtgg gcagcaccct tgctggagoc CggggCggCg tgcccgcgag aaccacaact gctgcaataa agcaagtaaa ataaccctga tttttgcggc gcacgagtgg gatgagcact tacactattc ttatgcagtg aaccgctttt acgacgagcg gcttcccggc ctggtttatt cctcccgtat
68. tgcagggcta ggcaccccta gatttgcttc ggtggctgca aagtacaagg catcggcatc aggaggtgca ccgaaaggcc atctagagcg ctttatttgt gcattcattt gagctcocgc aaaaaggaag ctcacccaga aacagcggta cgcggtatta actcaccagt aacactgcgg tgtaactcgc caatggcaac gaggcggata tgagcgtggg ggagtcaggc gaccaagttt tgataatctc cttcttgaga ccggatcaag gtagccgtag ctgctgccag tgaacggggg agaaagcgcc gggagcttcc tgatgctcgt ttttgctcac acctcccaca ggttacaaat actcatcaat tcccacacct tacaaataaa catcaatgta tccaatggcc tctgttccaa agcatgtacg gttcaaccct gcgtggtgat gatacatcca gaagatcgtg tgcctcctcc tgtggcggct tggcctgacc tgccctatgt aagagccaga caccggcgat gctaccaggt ccagacgagt ggattatatc caacaggcaa gccgcccaga gaaatttgtg tatgtttcag ggCCCggggg agtatgagta aacgctggtg agatccttga tcccgtattg cacagaaaag ccaacttact cttgatcgtt aacgttgcgc aagttgcagg tctcgcggta aactatggat actcatatat atgaccaaaa tocttttttt agctaccaac ttaggccacc tggcgataag gttcgtgcac acgcttcccg agggggaaac caggggggcg atgttotttc cctccccctg aaagcaatag gtatcttatc CCCCCtgaac gcaatagcat tcttatcatg acatccaacg gaagtccctg acatcaacgg aaagagtttg cagccacagg tcctggccat atggtgaaaa aatcatggtg CCCctctggg gagacctgta CCaggtgaag tgctgatgaa ctgggctact ggcccctgcc ttgccggcca gccgagctgg gctgatgaga catgataaga atgctattgc gttcaggggg atcctcaaat ttcaacattt aaagtaaaag gagttttcgc acgccgggca catcttacgg tctgacaacg gggaaccgga aaactattaa accacttctg tcattgcagc gaacgaaata actttagatt tcccttaacg ctgcgcgtaa tctttttc
69. tgcct ggttacatcg ttttaaagtt ctcagaatga gctgccataa tttgcacaac gtgacaccac caacaattaa tgctgataaa tcgtagttat attaagcatt gatctaggtg ccgtagaaaa ctaccagcgg accaaatact tgctaatcct gataaggcgc atacctacag tcggaacagg tgacttgagc gttcctggcc aggttttact ttgtttattg ttctagttgt ttttacttgc tttattgcag tagttgtggt aattcctgca gccggcattc ggtgtcttac tgatctccgt accgtgaacg gaaggccatc 481 561 641 121 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2121 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 4241 4321 aagaacatga ggcccagtgc acgccaagga aacctgagcg Cgtgcccttc gattcgagga ttcctggcta Caccgaggtt gcgccgtgct gtggacctga ctacttcaac acgacgatgg ctggagtggc gcccggcgcc ctcccaccaa gagctgcgga cacgctgccc agatgccatt tgaaaaaaat caacaacaat aatgtggtat caataatatt tectgttttt aactggatct ctgctatgtg cttggttgag ccatgagtga atgggggatc gatgcctgta tagactggat tctggagccg ctacacgacg ggtaactgtc aagatccttt gatcaaagga tggtttgttt gttcttctag gttaccagtg agCggtcggg cgtgagctat agagcgcacg gtcgattttt ttttgctggc tgctttaaaa cagcttataa ggtttgtcca tttaaaaaac cttataatgg ttgtccaaac tggccctgaa ctgagcaact gcaggtggoc tgcgcttcgt Caccacgcct gaagttcttc agagccccct gccgtggccg gatcacccoc ccaccggcaa aacaagcagg acatttcttc tgctgctcca tgcatcgtgc gcgcatccgg agctgctggc atgtcttgtg cttctagagc gctt
70. tgtagag ttgttaactt tcactgcatt tgtagaggtt ttaacttgtt ctgcattcta gactggctaa ttctaccccg ggagaccctg acgtgaactt gcccggtact tatgtcctcc gcaaggaccc atgttcacaa caccatcgag accagtacga aagcggctga cgacgtgaag tgggacctaa gaggaggcca ggaccggctg catccatcaa gagggcaaga agtcgtcttc agaagtccaa tgcagtgaaa gttaacaaca ctacaaatgt gegecgggac cacctggttg ctgacacaca aaatgcttca ttttgccttc ttacatcgaa ttaaagttct cagaatgact tgccataacc tgcacaacat gacaccacga acaattaata ctgataaatc gtagttatct taagcattgg tctaggtgaa gtagaaaaga accagcggtg caaatactgt ctaatcctgt taaggcgcag acctacagcg ggaacaggag acttgagcgt tcctggcctt gttttacttg gtttattgca ctagttgtgg ttacttgctt tattgcagct gttgtggttt gtgctacgag tgatcgocgc aacatctcca catcaagaag ccgagcccaa tctggcacca cctgttcggc ccctgtocta aagtacagaa cctgtccagc agatcggcgg accggctcca caagagaggc tcgacaagga aaagagctga ggatgccggc gcctgaccga gtggacgaca gctgtaatct aaaatgcttt acaattgcat ggtagatctg tatggttgct ctgactaatt ttccacagcg ataatattga ctgtttttgc ctggatctca gctatgtggc tggttgagta atgagtgata gggggatcat tgcctgtagc gactggatgg tggagcoggt acacgacggg taactgtcag gatccttttt tcaaaggatc gtttgtttgc tcttctagtg taccagtggc cggtcgggct tgagctatga agcgcacgag cgatttttgt ttgctggcct ctttaaaaaa gcttataatg tttgtccaaa taaaaaacct tataatggtt gtccaaactc aactacggcc cctgtacatg agcccgaact gtggtgctgc cctggacgtg ccggcctgcc aacagaacca cttcatcgtg tcccaacaat atcagagagg catcctccag CCggCagggt gagctgtgc
71. w toyobo co jp bio
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