腸内細菌遺伝子検出キット ―蛍光検出―
Contents
1. VREA RENERE REKT 2 L 2 PCR 22. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience Qtoyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order lifescience Qtoyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech osaka Qtoyobo jp URL http www toyobo co jp bio
3. 90 959 2 10 1 90 lt 9596 10 1 3 1 2 50 50
4. 1 1 5m 50ul 2 1 1 2 LEF 3 10ul 1 5m 1 50 4 2 lt 3 50 500u 3 B 1 5 0m 2 5ml 2 2 3 3 1 2 3 4 50 1 5 2 3 6 5 500ul 1 5m
5. m 3 PCR 1 5ml 95 C 12 000rpm TT ER 5 0ml PCR 1 5ml PCR 4 a invA Us Viva VTIVDD E Li ipaH 5 CO SERRA OO EURO FERE AB A
6. PCR 0 1 50 PCR 1 90 959
7. 6 P4 2ul 4 PCR PCR PCR UNG T or PCR 40 cycles 60 C 90 C Bio Rad MiniOpticon Applied Biosystems StepOnePlus PCR 2 PCR Bio Rad MiniOpticon 0 5 C step Applied Biosystems StepOnePlus 0 3 C step 0 3 C step
8. 6 3 1 MiniOpticon Bio Rad Tm 71 C 69 0 C 73 0 C 74 0 C 82 5 C 73 5 C 82 0 C 73 0 C 87 0 C d RFU dT PS EUN d RFU dT 70 75 a Temperature Celsius d RFU dT 70 75 a Temperature Celsius
9. 6 Tm ELA ESI ATE 69 00C Tm 73 0 C ind 025 J 2 1 i 73 5 C Tm 77 5 C E ds uw i MAMAS 78 0 C Tm 81 5 C O 81 5 C Tm 85 5 C P o1 T NW i Gad epe Mie MiniOpticon Bio Rad Tm Tm 71 C WEM US 0n 2 3 JAA F N REHE iii n BENE idi i Z JVzSEhbBA OREL ES
10. 9 Code No PCR 500 FIK 101 G1 100 FIK 201 G2 100 FIK 202 G1 G2 100 FIK 203 Label License Statement NOTICE TO PURCHASER LIMITED LICENSE This product is provided under an Agreement between Biotium and TOYOBO The manufacture use sale offer for sale or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium Inc U S Patent Nos US7 803 943 B2 US7 776 567 B2 Japan Patent No 5249013 The purchase price of this product includes a limited non transferable immunity from suit under U S Patent Nos US7 803 943 B2 US7 776 567 B2 Japan Patent No 5249013 and corresponding patent claims outside of the United States to use only the purchased amount of the product and components of the product solely for the buyer s own internal research whether the buyer is an academic or for profit entity in a manner consistent with the accompanying product literature No right to use sell or otherwise transfer a this product b its components or c materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its com
11. 3 2 PCR 480 50 24 000 20 C 9 54ml 10 x Primer Mix 20 C 1 2ml DNA E 6 UNG 20 C 60H PCR Master Mix EvaGreen dNTPs Mg DNA DNA EvaGreen Biotium Inc Biotium Inc 10 x Primer Mix invA VT ipaH PCR
12. DNA DNA UNG DNA UNG PCR PCR UNG 10 10 copy 20 C 10 ad Master Mix EvaGreen EvaGreen
13. 2 1 1 5m 95 C 5 2 12 000rpm x 3 3 2ul PCR 12 000rpm x 3 3 PCR 1 2 3 4 1 1 1 PCR Master Mix 15 9 ul 10 x Primer Mix 2 0 HI UNG 0 1 HI 18 0 yl 5 18ul PCR
14. 70 75 80 Temperature Celsius 7 0157 100 1000 50 PCR Ex mo 0157 a manu E wam sa PCR 8 10 PCR PCR
15. PCR UV BR 0 3 C step OO 10 U UV
16. Ideas amp Chemistry Code No FIK 301 TOYOBO CO LTD Life Science Department OSAKA JAPAN AS069K 1 2 t trenrunnnnnnnnnnnnnnnnnnnnnnnn 2 3 4 6 7 8 5 I I IIR HH 4 9 x vc 1L 1 PCR EvaGreen 50
17. ponents for any other purpose or a Commercial Purpose is hereby granted expressly by implication or by estoppel Commercial Purpose means any activity by a party for consideration and include but is not limited to 1 use of the product or its components in manufacturing 2 use of the product or its components to provide a service including without limitation reporting information or data resulting from use of the product or a its components for a fee or other commercial consideration 3 use of the product or its components for therapeutic diagnostic or prophylactic purposes or 4 resale of the product or its components whether or not such product or its components are resold for use in research For products that are subject to multiple limited use label licenses the most restrictive terms apply This product is for research use only Diagnostic uses require a separate license from Biotium Inc For information on purchasing a license to this product including for purposes other than research contact Director of Licensing Biotium Inc 3159 Corporate Place Hayward CA 94545 Tel 1 510 265 1027 Fax 1 510 265 1352 Email btinfo biotium com All names containing the designation are trademarks of Biotium http www toyobo co jp bio 9
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