患者及び浴場施設検体から複数血清群のLegionella penumophilaが
Contents
1. 6 1 29 332 333 2008 2 13 2 14 0214004 1 2 4 25 1 8 14 2010 5 4 23 10 7 242. 1 a Legionella pneumophila WREE 1 MIARE 6 PFGE sequence based typing SBT 1 r JERII Legionella pneumophila VAF L pneumophila BENERA L pneumophila 9 8 SG 1 2
3. 1 DHARE E OH TRIR D ORDRER OAIR Te Wahin aik A 3 pee OR 1 2 T818 0135 39 3 1 i Y M T812 8577 7 7 wees gt 2 EYRE EET a ena a T 162 8640 1 23 1 pH 500 mL 0
4. 4 2 8 PIC CRA LICR RRA ALEK GVPC A 1 GVPC C 1 pneumophila SG 1 6 4 3 BERIK 1 3 L pneumophila SG 1 3 12 SG6 3 12 PFGE 2 SG 1 SG6 2 1 2 Lane 25 4 4 Sequence based typing EIRIK 1 3
5. ST 537 ST 1212 ST 537 ST L pneumophila ni Ki BEKKA ST 1212 ST 537 aul t Ul ST L pneumophila 3 13 9 11 9s
6. SG 1 6 9 K 1 ST SG1 ST L pneumophila SG 6 1 PFGE SBT ST 537 Amemura Maekawa PFGE SBT PFGE SBT ST SBT PFGE discriminatory power PFGE SBT SBT SG 1 ST 1212 SG 6 ST 537 ST ST 138 ST 23 ST 1 ST 1212
7. 72 24 2 A FEH 5 29 A 400 ae 3 z LA a 3 1
8. 39 76 80 2012 Legionella pneumophila 1 3 SG 1 6 SG 1 3 5 6 Legionella pneumophila ean 3 900 13 800 CFU 100 mL sequence based typing 1 sequence ype ST 1212 6 ST 537 SARL MERRIEN ELE CIV ORR 5 ATER
9. YE H 1 0mgL 2 AR SLE FR LEK AR ERK GVPC BCYEa GVPC BCYEa GVPC BCYEa GVPC BCYEa LA 1 Bl A 2 B2 C 1 ID 11 1C 21 D 2 IL 1 an an a ie 1 3 900 WHEL 3 6 2 5 700 1 3 5 6 3 13 800 1 6 1DNA DNA pH 8 25 8 06 7 69 Thot X 1 GVPC 3 900 13 800 CFU 100 mL 9 10 CFU 100 mL cara L pneumophila SG IA 1 3 5 6 SG 1 6 pneumophila
10. ES 3 4 Wy VER 1 3 L pneumophila SG 1 SG 6 3 BR X L pneumophila SG1 1 PFGE PFGE SI Ve RM 200 V 50 14 C 21 o 3 5 Sequence based typing PFGE 2 SG1 1 SG6 1 ER 7 1 SQ1 1 SG6 1 2 SQ1 1 SQ6 2 3 SG1 1 SG6 1 sequence based typing SBT SBT The European Working Group for Legionella Infections EWGLI
11. http www ewgli org Sequence Based Typing SBT protocol for epidemiological typing of Legionella pneumophila Version 3 1 flaA pilE asd mip mompS proA zez4 gt eB SI 1 AARU GVPC BCYEo A 1 B 1 3000 rpm 20 GVPC BCYEo A 2 B 2 4 4 1 0 05 mg L A pH 2 2 5 GVPC BCYEo C 1 D 1 5 3000 rpm 20 0 2 0 4 mg L
12. L pneumophila SG 1 1 4 SG6 1 2 PFGE 2 5 SBT R 2 sequence type ST SG1 ST 1212 SG6 ST 537 ie iy L pneumophila 5 SG 1 6 L pneumophila PFGE SBT 1 L pneumophila 2011 Ul SHES He 38 2 1
13. 5 1 2 10 17 14 21 14 1212 2 9 1 2 10 17 14 21 14 1212 3 12 1 2 10 17 14 21 14 1212 17 6 3 13 1 28 12 9 3 537 1 20 6 3 13 1 28 12 9 3 537 2 24 6 3 13 1 28 12 9 3 537 2 25 6 3 13 1 28 12 9 3 537 3 27 6 3 13 1 28 12 9 3 537 SG6 Multiple serogroups of Legionella pneumophila isolated from a patient and a public bath Sachiko ICHIHARA Yoshiki ETOH Mitsuhiro HAMASAKI Koichi MURAKAML Shigeyuki TAKENAKA Kazumi HORIKAWA Akiyo ARAMAKI and Junko AMEMURA MAEKAWA Fukuoka Institute of Health and Environmetnal Sciences Mukaizano 39 Dazaifu Fukuoka 818 0135 Japan Department of Public Health and Medical Affairs Division of Public Health Higashikouen 7 7 Hakata ku Fukuoka 812 8577 Japan 2 Department of Bacteriology National Institute of Infectious Diseases Toyama 1 23 1 Shinjyuku ku Tokyo 162 8640 Japan The sputum of a patient that nearly drowned in a public bath and was subsequently diagnosed with Legionellosis and three spa water samples from the public bath were analyzed Legionella pneumophila serogroup SG 1 and 6 were isolated from the patient and L pneumophila SG 1 3 5 6 and untypable strains were isolated from the spa samples The concentrations of Legionella spp
14. 7 J Amemura Maekawa et al Microbiol Immunol 49 12 1027 1033 2005 8 23 6 Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ee ee ee mM Ny 2 ETH x2 oH Lane 5 7 Lane 9 11 2 1 6 Legionella pneumophila Lane 1 8 16 23 30 Salmonella Braenderup Lane 2 4 SG1 1E SG1 SG1 Lane 15 Lane 17 19 Lane 12 14 3 SG1 SG1 SG6 Lane 20 22 1 SG6 Lane24 26 2 SG6 Lane27 29 WHER 3 Legionella pneumophila sequence based typing 2 Lane __ ST No EB 2 1 2 10 17 14 21 14 1212 1
15. ranged from 3 900 to 13 800 CFU 100 mL in the spa samples Molecular epidemiological analyses of the isolates conducted using pulsed field gel electrophoresis PFGE and sequence based typing SBT revealed that the types of isolates from the patient and the bath were indistinguishable at the DNA level indicating that the patient may have been infected with the pathogen at the bath facility This is a rare case in which multiple serogroups of L pneumophila were isolated from both a public bath and a patient Key words public bath Legionella pneumophila serogroup 1 serogroup 6 pulsed field gel electrophoresis PFGE sequence based typing SBT
16. 2 He ES um KY HRZ bE AT DT ABB 50mL 5 mL sic Hae pH 2 2 100 2 GVPC Hh GVPC Oxoid 37 1 C WER C7 1000 10 2 GVPC 100 mL GVPC BCYEa F BCYEo BCYEo YC APA FREI RUFF YY ZYE Oxoid polymerase chain reaction PC
17. R genus Legionella 16S rRNA gene LEG L pneumophila stp DA 2 oom SY foe eR te ES ea iy AI macrophage infectivity potentiator gene mip DDH DNA DNA 3 3 BERIA al GVPC BCYEa C 2 D 2 E
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