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Enterobacteriaceae (rplP gene) Quantitative PCR Kit

1. http www takara bio co jp 11 RR241A NOTICE TO PURCHASER LIMITED LICENSE L11 SYBR Green This product is covered by the claims of U S Patent No 5 436 134 and 5 658 751 and their foreign counterpart patent claims Takara PCR products containing SYBR Green are sold under license from Molecular Probes Inc only for the usage in Real time PCR for internal research purpose These products are not to be used for the p
2. 10 2 V 2 DNA 2 1 ml 8 000 Xg 5 NucleoSpin Tissue DNA 100 ul 1 1 ml 1 5 ml 2 8 000 X9 5 3 Buffer T1 180 ul Proteinase K 1 25 ul 56C 1 3 5 Buffer B3 200 ul 70 10 11 000 X g 5 gt 99 210 ul 7
3. 0 2 ml PCR 4 PCR PCR PCR PCR 95 30 2 Step PCR 35 95 5 60 C 30 FAM SYBR VI VH1 rpIP Positive Control rplP Positive Control DNA OD2eo 1 X 105 copies ul
4. 10 BPW 2 VRBG PCR ER http www takara bio co jp 10 RR241A Vi 12 19 PCR Vi SYBR Premix Ex Taq GC RRO71A B Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 NucleoSpin Tissue 740952 10 50 250 Bacteria tuf gene Quantitative PCR Kit RR240A
5. 4 3 1 2 3 http www takara bio co jp 4 RR241A V 10 g BPW 90 ml y 4 1 ml 0 1 g 8 000 X9 5 y NucleoSpin Tissue DNA 100
6. 1 g PCR 0 005 g 200 1 g 10 g 5 R7K 90 ml 1 ml 0 1 g DNA DNA 100 ul 0 1 g y 1 20 PCR PCR 5 0 005 g http www takara bio co jp 8 RR241A VI 2 1g 1 copies g 2 1 9 copies g 1 1 g 1 copies tube 2 1 copies tube 3 1 9 rplP Positive Contr
7. 2 5 a ae 1 SYBR Premix Ex Taq GC e SYBRe Premix Ex Tag GC E ae 2 3 SYBR9 Premix Ex Tag GC SYBR9 Green
8. DNA PCR DNA L 25 ul 100 1 SYBR Premix Ex Tag GC 1 2 X conc 625 ul X 2 2 rplP Primer Mix 5 X conc 250 ul X 2 3 dH20 1 ml 4 ROX Reference Dye 2 50 X conc 50 ul 5 ROX Reference Dye II 2 50 X conc 50 ul 6 rpIP Positive Control 1 X 105 copies ul 100 ul 7 EASY Dilution for Real Time PCR 1 ml x 2 1 TaKaRa Ex Taq HS dNTP Mixture Mg SYBR9 Green 2 Life Technologies PCR StepOnePlus Real Time PCR System ROX Reference Dye 7500 Real Time PCR System 7500 Fast Real Time PCR System ROX Reference Dye Thermal Cycler Dice Real Time System 7e ER http www takara
9. ER http www takara bio co jp 9 RR241A 6 5 5 BPW 10 HER VRBG 37 48 96 PCR 2 qPCR VRBG log copies g log CFU g ERES A 7 12 6 58 10 BPW a a 2 gt E j a Jo Aa 0 i P E O a 54M lt 2 2 a Hx g a Yo 1 6 8 10 VREG log CFU g 3 VRBG CFU g PCR copies g VRBG PCR 1 2 VRBG
10. B5 740952 10 Wash Buffer B5 concentrate 4 ml 96 100 16 ml http www takara bio co jp 6 RR241A V 3 PCR V 2 DNA 5 pl PCR 1 3 rpIP Positive Control EASY Dilution PCR 1 5 ul 1 1 X 105 copies ul rplP Positive Control 2 1 X 104 copies ul rplP Positive Control ul EASY Dilution 45 ul 3 1 X 103 copies ul 2 0 1 X 104 copies ul 5 ul EASY Dilution 45 ul 4 1 X 102 copies ul 3 0 1 X 103 copies ul 5 ul EASY Dilution 45 ul PCR copies g g VI 1 copies tub
11. bio co jp 2 RR241A I 20 80 SYBR Premix Ex Tag GC Package1 4 6 80 C 4 80 20 4 6 Package2 20 I lt gt BPW lt DNA gt NucleoSpin Tissue 740952 10 50 250 gt 99 56 70 lt PCR gt PCR Thermal Cycler Dice Real Time
12. ul y PCR rpIP Positive Control y y 5 ul 0 005 g y PCR y PCR 100 ul 0 1 9 1 20 PCR Seran AAA 0 005 9 U http www takara bio co jp 5 J PCR RR241A V 1 2 10 9 90 ml BPW 1 10g 2 9 90 ml BPW 3
13. NucleoSpin Tissue Column Collection Tube 6 11 000 xX 9 1 Collection Tube 8 1 Buffer BW 500 11 000 X 9 1 Collection Tube 2 Buffer B5 2600 ul 11 000 X 9 1 Collection Tube 9 11 000 X g 1 10 1 5 ml 70 Buffer BE 100 1 11 000 X g 1 BROT DNA 4 20 1 Proteinase K 740952 10 Proteinase K 6 mg Proteinase Buffer PB 260 ul MATH Proteinase K 20 6 x 2 Buffer
14. System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies StepOnePlus Real Time PCR System Life Technologies 41 73 4 http www takara bio co jp 3 RR241A IV dci RUT 2 3 BER 121 20
15. e copies g copies tube copies g 1 19 2 1 o 20 ul 1 ul Thermal Cycler Dice Real Time System 1 SYBR Premix Ex Tag GC 2 X conc 12 5 ul rpIP Primer Mix 5 X conc 5 0 ul dH20 25 ul 5 0 ul 25 0 ul Life Technologies PCR 1 SYBR Premix Ex Tag GC 2 X conc 12 5 ul rpIP Primer Mix 5 X conc 5 0 ul ROX Reference Dye or ROX Reference Dye II 0 5 ul dH20 2 0 ul 5 0 ul 25 0 ul x StepOne Plus ROX Reference Dye 7500 Fast Real Time PCR System ROX Reference Dye ll http www takara bio co jp 7 RR241A 3 SH DNA 3 2 DNA 5
16. nd including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L60 Intestinal bacteria PCR Detection Licensed under the pending JP patent application No 2012 35637 M57 LA Technology This product is covered by the claims 6 16 outside the U S corresponding to the expired U S Patent No 5 436 149 TafdR4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp SII A ARN 4L v201308Da
17. ol X 200 1 g copies g VI 3 5 TSB TSA 1 ml PCR Proteus mirabilis JCM 1669 Klebsiella pneumoniae IAM 1063 Citrobacter freundii IAM 12471 Enterobacter aerogenes IAM 1183 Escherichia coli IAM 1137 10 28 E E 4 a lt Proteus S 6 5 r co A Klebsiella A SP 5 e A Citrobacter g Ae 3 7 4 Enterobacter A E coli lt R ior hi IE ad Dd cas FE ite 48 0 0 2 4 6 8 10 TSA log CFU ml 2 TSA CFU ml PCR copies ml TSA PCR 1
18. urpose such as providing medical diagnostic or any other testing analysis or screening services or providing clinical information or clinical analysis in return for compensations L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L46 SYBR Melting Curve Analysis The purchase of this product includes a limited non transferable license for all fields other than human or veterinary in vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology Inc and Roche Diagnostics GmbH to use only the enclosed amount of product according to the specified protocols No right is conveyed expressly by implication or by estoppel to use any instrument or system under any claim of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 other than for the amount of product contained herein L52 Rox Reference Dye Research Field Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 928 907 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim and no right to perform commercial services of any ki
19. ws RR241A Taha a Enterobacteriaceae rp P gene Quantitative PCR Kit v201308Da PCR 50S ribosomal protein L16 4 PCR PCR PCR SYBR Premix Ex Tag GC SYBR Green PCR GC F E F mp L_LLLLTLTITLTITITILULUIIIIIIII 2 F F eM F a rr rs p F F 3 Pi OO TA Dd A O e O o kl lllllllll 1

Enterobacteriaceae (rplP gene) Quantitative PCR Kit

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