Ver.6.1版 - GEヘルスケアバイオサイエンス
Contents
1. a N NE QO CO A QO NSR AE Ns gt le RN 2 A2. 8 3 1 System Control File Open Method Navigator 2 3 File Run 4 Start protocol Finish Next Finish OT Next 3 2 Hesult Name andLocahon gt gt Hun info Date 11372010 7 27 08 PM 093 00 User Default Method UNTITLED Fiesult Na result dd unique identifier to result name Directory DefaultHome Scouting subdirectory coe Name UNTITLED 001
3. NN NN CD RS Ey ia 8 1
4. 1 Administration Database Management Backup Restore archive Retrieve Helease Dbjects O Current backup selttings a backup is performed daily at 03 00 Hours 2 Backup Backup Now Roen abcaion CAProgiam Fles GE AKO 3 OK Ce 4 Go To Backup File UNICORN_MANUAL_BACKUP_yyyymmdd_hhm
5. System Control 1 Run Data Chromatogram Details PreC Pressure 2 Manual Instructions Farameters for System flow Flow rate 0 000 25 000 Sample fl 5 000 mmin A A1 Pumps and Pressures gt System Flow 5 de Pocono System wash 3 3 Wash settings ml min gt Execute Roe JR PreC PreSSU G F O Fracton collection 6 Save resul las Browse 9 B B1 a Pumps and Pressu res Gradient 100 B amp uto update parameter fields will be updated during method run Execute PreC Pressure A2 A7 B2 B7 B2 A2
6. 2 3 2 LD NLIA N 18 1027 11 10 114 11 0004 14 S1 S7 38 12 1
7. 0 NE TN 60 7 a 40822 TN 4
8. 7 1 2 NS 3 UV Cond PreC Pressure 1 4 8 End Pause Continue Manual Instructions Other gt Timer gt OO Acc yalume i 0 33993 00 5 min Disahled 4 2
9. 6 OK 1 2 AKTA avant 20 2 3 4 4 6 _ WV 10 2 2 1 UNICORN AKIA ovant
10. 6 20 1 System Contro System Settings System Settings 2 Fraction collection Fraction collector lamp Inslructions em Farameters for Fraction collector amp Conductivity pH Pumps and pressures Onn Air sensor Fraction collection Fractionation settings Last tube fd Peak fractionation parameters Fraction collector ternperature Cassette configuration a Delay wolumes 3 Mode On Off 4 Fraction collection Fraction collector temperature Instruclians Farameters for Fraction collector temperature Conductivity a pH Pumps and pressures on OO Air sensor i Target temperature 6 20 Fraction collection a Fractionation settings 5 3 C Last tube filled Feak fractionation parameters Fraction collector temperature Fraction collector lamp Cassette configuration Delay volumes 5 Mode On Off On 620 1
11. 1 UNICORN 2 IO9On 00plen 2 2 0 350OK 2 Tools 2 3 3 1 PTFE ETFE w1 W2 pH wW3 W 2 2 nn 20 lh 1 1
12. 3 Phase properties 7 3 2 M ethod Method Sethings Evilbreeon Settings 2 a Column selection y Show by technique Cation Exchange v Sampie Application Pe Colamn Wash e Col UIT type Column yalume 4 B57 m Pressure limit pre column 5 MPa UL02 20 0 1 EluWion we Column position Pressure limit delta column 0 30 MPa 0 02 20 00 PL 1 a Column Wash N RN se fow restrictor 3 gt Flow Hate Lnit mmn alumn position Position 1 EquilibeaSon YIONICOT Settings Flow rate Wavelengths 190 700 Monitor settin gs Flow rate 2 700 mMmn 0 000 25 000 ee pm Control the Flow to awoid overpressure Uy2 Uy3 Use manuall prepared bulfers Enahle pH monitoring Inlet amp amp InletB B
13. 1 Manual Instructions Pumps and pressures gt Pump Wash gt A1 B1 A2 A7 B2 B7 A2 B2 2 Manual Instructions Pumbps and pressures gt Pump Wash gt A2 B2 Buffer S1 3 Manual Instructions Pumps and pressures gt Pump Wash Buffer gt Pumps and pressures gt Pump Wash S1 Pump Wash Pump Wash Pump Wosh 14 3 5
14. AKTA avart 2 2 UNICORN UNICORN 4 Administration Method Editor System Control Evaqluation 2 3 lt Windows 7 gt RR Administration SS System Control Evaluation Method Editor lt Windows XP gt a Administration Aa Ewaluation s System Cnntral 2 Method Editor UNICORN UNICORN
15. Pump Wash Inlet A Inlet B Q Inlet Direct inject Sample pump load 10 ul 100 25 MPa 500 1 ml 2 ml 10 MPa FE 5 ml 1 MPa ww 150 ml y 10 ml 50 ml 4 MPa 150 ml 2 MPa
16. UNICORN Log On 2 OpM6e OK Default omair Ccess dministrators Start Administration System Control hiethod Editor Ewaluation 4 2 3 gr Reqdy Ready Ready mpawse lk Continue UNICORN AKTA avant gt mr LC 1 Connected systems 1 selected max 3 System name System 1 System Control System Connectto Systems Connect to systems 2 System name Control 2 O6K
17. USB I 1 42 3 2 Printer Micrasoft xPS D ocumen L Write File Print Print Chromatograms mwmews 2 Print format Ta ed 3 Use thick ines hromataarams in each row 4 Preview Customize Report Oh Preview ca File Exit 5 E Printer Properties OK
18. Manual Instructions Pumps and pressures gt Pump wash S1 Buffer HEnd 8 5 Inject System pump Sample waste pump load LoopE SaP V9 Inj sap V9 Inj saP V9 Inj Manual load Inject System pump waste Sqmple pump load Sample pump waste Direct inject 5 1 1 LoopE LoopF 2 Syr 3 W1 W2
19. Yes 12 1 No No eee 1 421 2010 7 44 07 PM 203 904 5 OK ECC 2 Do ou want to contin em 6 Restore successful OK 41 13 13 1 End 0 02 MPa 1 System Contro System J Cqlibrate Caqlibratton Galibration System3 Monitor to calibrate EC Calibration procedure Sets the system pressure to zero jon System pressure iS used to calibrate the system pump pressure monitor The system
20. 2 RS all Control System Calibrate Calibration Calibration for pH electrode bulfer 1 TUNE PS als a Bien inde th OIRO pH for buffer 1 alibral Using two pH calibration solutions It pH for buffer 2 Last calibrated nn 2009 01 13 10 52 07 01 00 Calibrated electrode slope Assymetry potential at pH 7 my Y 3 pH pH 4 Monitor to calibrate pH Prepare for caqlibration 42 5 pH for buffer 1 pH 6 10 ml pH Cal Current value Calibrate 10 ml pH cal
21. Delete Segment 7 5 2 7 8 co Column wosh gamma ears J 2 O Use the same low rate as in Method Settings Use the same inlets as in Method Settings M Flow rale 27h mmin OU 25 000 Inlet amp 1 wv Sampte Application 1 InletB B1 y 1qnn B 0 100 v Wosh U ntil tota VOIU me is Fil the systern with the selected buffer Pe Wash untl lt 2 5 0 Is gw 9 8 ax A 9 W less than D0 mau 8000 0 E000 0 7 5 2 Stability time 100 min 0 02 1000 00 accepted UV fluctuation mall 0 00 EOO0 00 O Maximum wash walume Cy Delete EE using fraction collector Fractionation destination Dut 1 O OO in
22. Flow path Injection valve gt Directinject gt Execute Pumps qnd Pressures gt Sample Flow 1 ml min gt Execute 2022302 ENDN Pumps and pressures gt Pump Wash gt Execute 6 ENDN Y Other Timer gt 2 20 10 12 1 eee 8 7 12
23. Manual Instruction 86 LU 9 2 Customize Customize 29 8 6 1 Run Data Rn pata Run Data lp state Re volune Conc B gt gt ean Re flow gt gt pressure gt gt wr position gt gt ee direction gt gt UE gt gt EE gt gt gt gt inlet gt gt yalye gt gt inlet gt gt inletB gt gt 6 sample inlet gt gt 1 Run Data Groups Block yolume RE time nme flow gt gt pressure gt gt ES gt gt Xaxis YAxis HunLoq 2 Rd a Legeeee 3 OK Dalta pressure PostC pressure Uy 1 Uy 2 Uy 3 pH BufferPra pH Conc 91 Canc 2 Canc 3 Canc 4 Cand Cond re 8 6 2 Curves
24. 6 OKh 3 3 10 0 9 0 TR CR CO ER NN EE 10 1 1 012 10 1 1 4 1 J O 1 2 Maqnual Instructions Pumps and pressures gt PumpWash gt Execute 3 Maqnual Instructions Alarms Alarm pre column pressure gt High alarm Max pre column pressure gt Execute Alarms Alarm delta column pressure gt High alarm Max delta column pressure Execute Flow path gt Column Position Position 2
25. File Edt view Phases Toos Hep ge Raa Ctr 0 1 Method editor File Open Method Navigator 5 2 a TT 3 File Export gt to UNICORN gt Export Method Re et La off DeFault to UNICORN Exi UNICORN 4 OK Fle Edit View Inteqrate Operatinns Procedures Tools Help gt 1 Evaluation File Open gt Result Navigator Me 2 New od 3 File Export gt ToUNICORN gt Entire Result 4 2 OK Import Log OFF DeFault Externally Exit UNICORN 12 2 UNICORN
26. Method Manual AKTA qavant 25 14ml AKTA qvant150 5ml 2 4 2 4 62scemmn chmMm 1aC4mWmin 1C6mWmn 1 1MmMmm 5 lt 25 ml min 6 25 ml min 5 50 ml min 2 10 ml min mmimn 102 mm 10 AKTA qvant 25 1 mmin AKTA qvqnt 150 2 mmin 18 1027 11 10 0
27. Yes No Cqncel 2 Windows 3 4 1 11 1 2 20 20 20 ES MM NUM 11 2 1 1 1 20
28. 4 B B2 Flow path gt InletB gt B2 gt 5 A A2 Pumps and Pressures gt Gradient gt 0 B Flow path InletA gt A2 6 End Buffer S1 7 Manual Instructions Buffer Flow path gt Injection valve gt Directinject Pumps and Pressures gt Sample Flow 5 ml min PreC Pressure 8 S1 Flow path gt Sampleinlet gt S1 Ss2 S7 9 End 0 05 MPq 1 Moanual instructions Select column type Select 2 Select co
29. 518 48 566 Ll 142 pl 48 l 190 gt 1 System Contro System Settings System Settings 2 Fraction collection Delay volumes Instructians Farameters for Delay volumes Conductivity Detector Frac 0 10000 p a Pumps and pressures 1807 Air sensor i Detector Hullet yalye 0 10000 Fraction collection a Fractionation settings Hl Last tube led Restrictor volume 0 10000 Peak fractionation parameters Ot ga Fraction collector temperature Fraction collector amp pH cell volume 0 10000 Cassette i 3 20 7 7 1 Method editor File New Method 7 2 Predefined Method Affinity chromatography AC Anion Exchange Chromatography AlIEX Cation Exchange Chromatography CIEX Chromatofocusing CF 2
30. 1 1 1 13 14 15 File Run 16 Start protocol File Save Save As System Control File Open Method Navigator Variable List Next Finish Finish 84 30 10 2 hE Run 1 File Exit UNICORN 0 O Recent Method Runs LaoqnofF DeFault
31. Auto CrsBuOemtVemmkeoekn Ci Tn DOS Sstem law hram 1 Traininn laboration 009 Syst Chrom 1 Training labor Fixed ST 3 Sample flow_Chrom 1 Training laboratior MA k 14 Sample pressure_Chrom 1 Training labor 1 eft Axis O DS PreC nressure_ hram 1 Trainina haral O16 DeltaC pressure_Chrom 1 Traning labors O01 UY 1_280_Chrom 1 Training laboration 1001 3 UV Allwih this unit BE 5 Y Right Axis 6 OK arrbo lehonacgams lt Nane vv 9 2 3 X Lurwe Style and Color Edit Texts Layout Library N Y Axis Ams Curve Peak Table 1 Axis oe 2 X a Auto Fixed
32. Execute Pumps and Pressures gt SystemFlow gt Execute 3 UV Cond Pressure END 3 4 Mganual Instructtons Pumps qnd Pressures System Flow 5 ml min gt Execute Flow path gt Outlet Valve gt Fraction collector gt Execute Fraction collection gt Accumulator wash gt Execute Flow path OutletValve gt Execute 0DU EL SENDN 5 1 syr 3 5 2 0 5 lt 1M
33. Axis scale Adjust retention zero to injection number ES Fised ml UC 0 Adiust retention zero to injection number 1 Omin mW 3 OK apply to all chromatagrams 9 24 1 2 3 Reset zoom 9 2 5 Addtet 2 3 lt text gt lt text gt 93 El
34. Nome ED Save a ED Lab1 Manual 5 26 2009 3 08 0 Delaul Save Lab2 kianual BE B 20H 2 46 54 Defaul Lab3 kanual 65200912 33 2 Default Lah4 anual 65 2009 1 433D Default pH scouting br3 2009 1 18 41 Default pH scouting S52b 2009 3 13 5 Default lt 1 Name UNTITLED Sustem Lab1 8 8 1 0 45 um pH 8 1 1 1 Manual loagd A 3
35. 5 2 0 ml LoopF LoopE 5 3 18 6 R 6 1 3 ml 28 9564 27 8X5 40 8 ml 28 9564 25 6X4 24 15 ml 28 9564 04 5X3 15 50 ml 28 9564 02 3X2 6 28 9542 12 24 48 96 50 ml 28 9803 19 11X5 55 250 ml 28 9818 73 6X3 18 1 1 1
36. W1 i a 5 8 1 2 8 2
37. 1 20 A B 33 2 1 2 A1 B1 2 1 A1 B1 9 2 AI OO ma 3
38. 50 ml EE 650 ml 6 ON 4 GE 6 50 ml 250 ml ww 50 ml 250ml 19 5 6 mrrgzr mmmmmmgmmmm wmv
39. EE 0 e mmmmmmmmmmmm System control View J Fraction Collector Content 5652 pH AKAaem25 A oa UV pH AKTA avant 25 pH
40. Column CIP Column Performance Test Column Preparation Desqlting DS 7 Gel filtration GF Hydrophobic Interaction Chromatography HIC Reversed Phase Chromatography RPC System CIP System Preporation OK Method editor Phase Library AKTAavant2 X Phase Properties Text Instructions T Column CIP Method Settings Method Sethngs Column selection Hesult Name amp Location Column Performanca Test Show by technique Cation Exchange Start Protocol Equilibration Column type HiScreen Capto 5 Column Preparation Method Notes olumn Properlies Calumn yolume 4557 ml Column Wash Sample Application Pressure Imi pre colum 0 50 MPa 0 02 20 00 Unit selection Pressure limit delta column 3n MPa 0 02 20 00 Elution y Method Base Unit Cy Use flow restrictor Column Wash Flow Hate Unit mmin Equilibratio Column position Pasition 1 NZ Monitor settings Sample Application Elution
41. A TI NZ AKTA qavant 5 2 2 Syr tn Loop tn W II 8 2 2 9 AKTA qvant 25 0 001 25 ml min 20 MPo CA Pre 0 00 MPa AKTA avant 150 01 150 ml min 5 MPo DeltaC 0 00 MPa i PR EN a Out Waste 1 2 FC em AKTA avant 25 01 25 m min 10 MPo mmooemsm AKTAqvqnt 150 0 01 150 m min 5 MPa pH 0 00 PU IE 2 0 NN 8 A A1l A7 Q B B1 B7 Q Buffer S1 S7 A1 B1 Buffer a B Oo BufferPro 4 pH
42. Fingertight 28 4010 81 AKTA qvant 25 0 5 mm 0 75 mm PEEK AKTA qvant 150 0 75 mm 1 mm PEEK 1 6 mm PTFE 18 1112 46 1 16 female 1 16 female 11 0003 39 4 1 5 2 1 2A 2B 2 2B od essue lt Patametersfor Column postion N Iniection valve sk 2 6 Cc 0 O Inet A Flow Direction Inlet B Down flow Up How a NN NN pH yalye 3 Manual Instructions Sco et
43. 3 995 AP 4 gt 0 6 MPd System pressure Sqmple pressure Pre column PreC pressure Post column PostC pressure Delta column DeltaC pressure gt DeltaCP System pressure 0 3 MPQa A PE 0 5 MPa 0 5 MPo Samplepressure Pre column pressure Delta column pressure 0 Z MPa 5 Down Flow Up Flow UV 190 700 nm UV Vis 3 2 mm pl 0 5 mm 1 uD 10 mm 8 uD 0 01 999 99 mS
44. A2 B2 TTP Buffer S1 2 lt 7 Buffer Flow path gt Sampleinlet gt S1 S7 2 5 10 BufferPro Q Pumps qnd pressures gt Pump Wash BufferPro Q inlets Al gt Execute 34 Pump Wash
45. 4 Inject System pump Waste sap vg inj Inj SoP V9 inj Manual load Inject System pump waste Sample pump waste Direct inject Sample pump load Manual load Inject NR 0 6 6 Sample pump waste Pump Wash Sample inlet system pump waste
46. 2 3 OK Fun Data Groups Run Data Color Curve Style and Color XAxis YAxis RunLog Select curves to display D01 UY 1_28n 02 uy2 0 03 Uy3 0 04 Cond 005 Cond DOE Canc B nH pH O08 System flow 009 System pressure OO Cond temp O11 Fraclian 12 Iniection 013 Sample flow 14 Sample pressure 15 PreC pressure DE DeltaC pressure 020 PostC pressure 22 Hun Log 02 Conc Q1 028 Conc 2 023 Conc 3 030 Conc 4 HE Canc 8 6 3 Y 1 Vadls Fun Data Groups Run Data Color Curves Curve Style and Color Axis YAxis HunLog ee Select curve to modify axis scale for Do1 Uy 1 280 Scale for curve number DO1 1 2 cg oe ME ME DOE Conc B Auto ne EE Ate ll with This Unit ODO Cond temp D1 Fraction DZ Inection D3 Sample flow ki
47. 9 pH for buffer 2 2 pH 10 10 ml pH Cal 11 Current value Cqlibrate 12 Close 3 9 J 0 pH 0 5 MPa Post column pressure 0 5 MPa pH Continue 13 3 pH pH pH 1 System Contro System J Caqlibrate Caqlibratton 2 Monitor to cqlibrate pH Prepare for calibration 3 10 ml pH Cal
48. Ye a9 ENew Method Queue 1 Method editor File Import gt Import Method 2 OK 3 Import Method Name 4 Import Recent Methods i LogofF Default Exit UNICORN 39 New 1 Evaluation File Import gt Entire Result oe 2 OK he 3 Import Result Name New Folder Edit Wiew Intearate Operations Pracedures Tools 4 Impot Exit UNICORN 12 3 1 Method editor File Open Method Nqvigator 2 Delete 3
49. PAR SS Fill Ihe swstem with Ihe selected buffer Equllibraon N A Equilibrate until Fill the system with the selected buffe Conductivity greater than nnqlmszcm 0 1000 00 0 00 14 00 mall 0 00 EOO0 00 mS cm 0 00 300 00 ccepted pH fluctuation Aaccepted UY fuctuation Aaccepted conductivity fluctuation Siqnal stable for min 0 02 1000 00 NN Maximum equilibration yalume 22 7 5 5 1 Method Settings Dy Sqmple Sample Applicaton 2 EqulllbraWon A ICOtI on Use the same flaw rate as in Method Settings PP 5 2 Flow rale 2g7U mmin HUUO 25 000 Sampie Application F oe 5 1 f Iniect sample from loop Fill the loop using Manual load v ect sam D eTrom IOO D Loop tpe Capillary loop ml v Inject sample directly onto column i Elusion 7 D x Sample inlet y Fill loop with Emyeerh 1m Sample yalume _ 000 ml Em pty loo D with a ise the same inlets as in Method Sett
50. ea 2 Method editor File New Method 2 3 Predefined Method Column Preparation Method Descipton The column is filed with buffer solution Select inlet positions a 3 7 a Enter the solution identity wolume Fow rate and incubation time O K MK 0 O Bu addina steps seweral preparation solutions can be used 4 0 Method Settings Method Sethngs Column selection Column Column selection Show by techniqle Cation Exchange position Ko Column type HiScreen Capto 5 Fiesult Name amp Location Start Frotocol NN3 nn 5 Start Protocol Mo Rm Pressure limit pre column 5 MPFa D 02 20 00 EEE Ke O Pressure limit delta calumn 30 MPa U 02 20 0 cv _ EK Use flow restrictor 6 Variable List i Flow Fiate Unit mlymin v OK O kMelhod ltems to display at method start Fraction Collector Current settinayitem description Yariable List Displays a list of all method wariables and allows PE changing of wariable yalues Text Instructions Not
51. Es bt OFF NG UNICORN Bp URL AKTAdesign http www gelifesciences co jp tech_supporVakta index asp AKIA http www delifesciences co jp tech
52. FOO Cond temn_Chram 1 Training laboration 1 001 3 OK 1 Fraction_Chrom 1 Training laboration 1 O01 2 Inieclinn_ hram 1 Traininn laboration 1 O01 13 Sample fow_Chrom 1 Training laboration 1 001 4 Sample pressure_ hrom 1 Training laboration 1 O01 Ds Pre pressure_ hram 1 Trainin laboration 1 O01 DE DeltaC pressure_ hram 1 Traininn laboration 1 II1 02 PostC pressure_ hrom 1 Training laboration 1 D01 022 Hun Log_Chrom 1 Training laboration 1 O01 027 Conc Q1_Chrom 1 Training laboration 1 001 028 Conc 92_Chrom 1 Training laboration 1 001 023 Conc Q3_Chrom 1 Training laboration 1 001 030 Conc 4 Chrom 1 Training laboration 1 001 034 Frac temn_Chram 1 Training laboration 1 001 apply to all chromatograms 9 2 2 Y mmmrm m Header CurweNames nis Axis Curve PeakTable l Y AxiS 7 9 Select curve to modify axis scale for HREF RSE Scale for curve number DO1 ag Cmam TT pe DD Aulo 2 FP Pi 003 UY 3_0_Chrom 1 Training laboration 1 OI Min Mag 9 at P Bed Al Cond_Chrom 1 Training laboration Pe DOE Conc B_ hram 1 Trainina laboration 1 Ot PT
53. 5 Select column Disable column logging for the run 8 6 lot i Cancel Enter ID O Select ID Disable calumn logging for this run Appl to al methods with the same column type kethods Remark 28 3288 13 12345B78 IED UU OONS HiPrep 251 Column Barcode Column Handling a 6 New 20 eS 0 2 5 Continue 7 OK 8 Enter ID 2 2 Enter or select a calumn In
54. Column Wash 2 0 200 the total volume is 7 5 2 lt Elution 2 WM lsocratic elution Gradient elution GU 2 Type Linear 1Step Step with fl Step Step with fill B Target 9 B B Length Linear Column Wash Use the same flow rate as in hlethod Settings Flow rate 2 700 mlzmin 00 25 000 wy ash ul the total yolume is the following condition is met Fractionate using outlet yalye using fraction collector in waste da not collect Elution Use the same flow rate as in hethod Settings Flow rate 2 700 mimin OD OO0 25 000 Up flow UY less than Stability me Accepted UY fuctuation kasirmum wash wolume CY Fraction
55. 0 OA 1 AKTA avart 2 OS UNICORN Ei LICOR 3 3 Log On User Name Default 5 Password default Ok Option 9
56. 2 Me UVS9 Fractionation destination Peak fractionation volume 1 Fixed outlet Fractionation destination RU 0 Advanced Settings Peak Fraction Settings level slope level and slope level or slope usind fraction collector 3 Fractionation Type Fixed volume fractionation 3 Fractionation destination Fixed fractionation volume 1 Peak fractionation 20M 2 US eg Fractionation destination Peok fractionation volume 1 Fixe
57. 2 3 ml 8 ml 15 ml 50 ml 4 2 50 ml 4 250 ml 1 96 Whatman 7701 5200 Eppendorf 951033405 0030 501 306 Falcon 353966 Greiner 780270 Porvair 219009 Seahorse 201240 100 S30009 487 Whatman 7701 5500 Seahorse 201238 100 S30004 24 Whatmqn 7701 5102 Seahorse 201272 100 S30024 2 3 ml 10 5 mm 11 5 mm 50 mm 56 mm Nunc 361239 8 ml 12 mm 13 3 mm 96 mm 102 mm VWR 60818 860 15 ml 16 mm 17 mm 114 mm 120 mm BD 352096 50 ml 28 mm 30 mm 110 mm 116 mm BD 352070 3 1 4 250 ml Nolgene 2110 0008 Kautex 303 70531
58. Yes 1 Evaluation File Open Result Navigator 2 Delete 3 Yes 12 4 1 Method editor File Open Method Nqvigator 2 Rename 3 Enter 1 Evaluation File Open Result Navigator 2 Rename 3 Enter 40 Open ename Expand Collapse Properties Open New Folder Ctr N Rename Copy Ctr C Cu CtrlT Delete Dal ExpandjCallapse Properties 125 UNICORN 6
59. 4 pH Cal 5 Close 6 2 pH 3 pH pH pH pH pH pH pH 43 134 System Control Manual Instructions Fraction collection gt Frac cleaning positi
60. cm pH pH FR 902 pH pH 0 14 pH 2 12 0 1 pH pH pH FR 902 UV 0 2 MPa pH Waste Frac Out1 Out10 12 AKTA qvant 25 0 1 250 ml AKTA avqnt 150 1 250 ml 6 24 48 96
61. pressure is set to zero Detailed infomation 1 Loosen the capilal eine nected to the ary Last calibrated on 2009 02 20 15 36 40 01 00 em DU SSU e refer to the Means or the locatio 2 Se Im 1 ImD pressure De she Reset press ie button The new Kr n date and the pressure Value are Close 2 lt SVSterm pressure Sqmple pressure PreC pressure PostC pressure 2 ll 2 I Oul 3 Monitor to calibrate Reset pressure 4 Close 5 13 2 pH H 2 pH 3 M pH 4 1 pH pH
62. 2 Save Partial Result OK 8 5 System Control Run Hold Continue Pause Continue Pause Continue Hold Pause m End 2
63. 4 10 20 ml 3 AA 0 229 A1 5 B 2 2 4 13 A2 B2 6 9 A3 A7 B3 B7 System Control 6 Maqnuaql Execute Maqnual Instructions Manual Instructions es E 7 Flow path gt Inlet A gt A2 gt oh 8 Flow path InletB gt B2 i am 9 2 5
64. 6 3 Wavelengths 190 700 nm Flow rate 2 700 mmin 0 000 25 000 Uy1 280 nm System CIP Cantrol the flow to avoid overpressure uz2 ss Column Wash Uy 3 nm System Preparation Ty Use manually prepared buffers Enable pH monitoring es Inet amp A1 InltB B1 Equilibration Use BufferPro automalic buffer preparalian Enable air sensar alarm Hecipe Inlet amp Inlet B Sample inlet 2 H 2 0 7 0 ecommen ded Canc Defined by recipe for mullicnmpanent uffers Column Logbook nable logging of Column Performance Test m Gradient Phase Block 0 00 nh Method Settings Main 4 Gradient New Method Sustem Lab1 Predefined Method Cation Exchanne Chromatography CIE klethod Description After equilibration and sample application positively charged proteins are adsorbed to the columr ligand After a wash to remowe unbound sample elution Is performed using a gradient of increasing salt concentration of for example NaCl Finally the calumn is washed and re equilibrated with start buffer Cancel 1 Phase library 2 2
65. E Xenable pH monitoring O Use BufferPro automatic buffer preparation Enable air sensar alarm pH Hecipe 2 Ineta NN N Inlet B pH pH Sample inlet nH 20 7 0 recommended 2 7 Conc Defined by recipe for multicomponent buffers Column Logbook Enable logging of pH MM M LH a Performance Test BufferPro Use BufferPro Recipe pH Conc AIEX mix CIEX mix 7 4 ee Equilibration Fauiibraton 2 2 2 9 Heset UV monitor recommended if the equilibration nccurs before the purification vy Use the same flow rate as in hethod Settings se the same inlets as in hethod Settings eae the total volume js Flow rate 2700 milemin 0 000 25 000 Inet amp A1 v Inlet B B1 nl B DDO 100 0 Bluaon
66. GE Healthcare Life ScienceSs AKTA avant 25 AKTA qvant 150 UNICORN 6 3 1 O 5 702 2 4 2 11 3 C et 13 Md 16 5 lt _11 _ 4 4 3 KC 44 3d 44 4 i I 18 I ds I 22 NT STO 0000000 19 7 M NN 21 SM i AGG GN cc 27 9 Uk ke cg E 32 10 Lt ee 34 38 Ub RT OTOP OTC D070 0 39 13 ll 42 GE TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com 1 Revised 2013 12 27 GE imagination at work
67. Test System Preparation Column Preparation 4 Column Preparation Column Wash Elution Equilibration Sample Application System CIP Solution note Pause to manually move all inlets to the selected solution Flow rate Wolume per position 2D 00 ml B inlets Al Al 1000 mMmn 0 000 25 000 Calumn Position Dutlets Sample inlets Dthers Bulfer Byrpass Huwraste inlets 4 20 ml per inlet 51 Position 1 Fraction callector BE ARAN mm pe hs 52 Pasition 2 nut 1 Injectian yalye with capillary loop 53 Posiion 3 Ou2 Loop cleanina volume 10 00 ml 54 Posiion 4 Ou3 OM 1000 55 Position 5 nut4 Far camplete cleaning SE Out5 select a sample inlet clean the manual injection 57 ut port using a syringe ou r Hut8 out Fraction collector accumulator out10 Total salulion yalume used in this phase 3 ml Incubalian lime min Syr 3
68. a Select which axes to displaw DiS Beetle pose AE 4 3 UV ee All with this unt ee 5 Y Right Axis i cea 6 OK 30 8 6 4 1 X Axis 2 a ES Auto Fixed Window 3 OK 1 Fun Data Groups FunData Color Curves Curve Stule and Color Base Iime volume Auto Mini Fised ml OO Window ml 1 AaRIS YAwis RS FunLog w Less 9 1 1 Evaluat
69. ample inlet with RNN Wosh sample pump wrth Duffer fter sqmple application Buffer 7 5 2 23 7 5 2 Fractionate usind outlet valve 3 Frqctionation Type Fixed volume fractionation Fractionation destination Fixed fractionation volume 1 Peak fractionation
70. ation settings Fractionation type Fised outlet Fractionation destination Dut 1 vv Fixed fractionation olume Peak fractionation wolume O lsocratic elution Vnlume 15 cv Hradient elulian Start at 1 2 Note gradient delay is automatically added provided that the last qradient segment is linear Fractionate O using outlet yalve using fraction collector in waste do not collect Linear Step Peak fractionation destination B DO 100 0 Inlet Inlet B Fil the swstem with the selected buffer 200 Cy 1 w se the same inlets as in Melhad Settings B1 wv 0 0 B DO 100 0 mall EOO0 0 EOOD 0D mn 0 02 1000 00 mall 0 00 EOOD OD Inlet amp Inlet B Target B Length ID 100 Cw 50 0 10 00 100 0 5 00 Fractionation settings Fised fractionation wolume Peak fractionation olume CY only for isocratic elution Fractionation type Fractionation destination Peak fractionation destination B HU 10H ml 0 01 20000 00 ml 0 01 20000 00 dd Segment Fil the swstem with Ihe selected buffer ise the same inlets as in Melhad Settings Fised yolume fractionation dB deep well plate lt Advanced Settings i D ml 0 00 2 20 ml 0 00 2 20 Add Segment
71. d volume qnd peok fractionoation Fractionation destination Fixed Peok fractionation volume 1 Advanced Settings Start Position next tube next line next cassette skip two tubes Lost Tube Filed Pause Out waste Out 1 10 Peak Fraction Settings level slope level and slope level or slope in waste do not collect 7 6 Method Settings EquilibraSon Sampie ApplicaWion EqullibfaWon 7 PR Metnod Settings EquilibraWon Sampie Application Column Wash EquilibraSon
72. dividual Calumn type HiFrep 25410 Desalting ok _ ws Code lot exp ID I Column ID The Column has a uniTag has a fixed Code and exp lat Exh Column ID ris no o NE jn He has fe D Alias apional Technique Desaliing y Column type HiPrep 26 10 Desalting Use medium batch ID Get medium expiration date den 18 febiuari 2h09 Enter ID nde lot exp ID ke 9288 13 12345678 oooo 00 ooos SelectID O Disable column logging for this run 28 2B 3288 13 12345B7Z8B O00 D0 O005 HiPrep 251 Select ID le 4 Enter or select a column Individual Code lot exp ID ss Select ID 28 9288 13 28928813 0000 00 1234 HiPrep 251 263289 1312345678 00000 0005 HPrep 2510 Desaling E Disable column loaain ng TT TE HiPrep Desalin Rai 5 9288 13 28928813 ODD 0D 1234 HiPrep 2 2E10 De salting E OK 8 4 1 End End Run
73. es radient BufferPro Columns Eyalualian Procedures Method Informalian System Settings Calibration Hueslians Changeable Batch ID HesultName and Locaton A 5 Column Preparation Column Preparaton a a ee ee Vormeioy B1 nn own flow HuLwraste IM 3 5 35 9 Phoase Library System Preparation Method Settings Column Preparation 10 B1 Fraction collector Fraction collector occumulator 20 30 ml Volume per position Injection valve with capillary loop Loop cleaning volume 3 System Preparation This phase uses one solution Phase Library Method Settings Column CIP Column Performanca
74. ings Ineta A CD InetB B1 0 Ko Fil the system with the selected bulfer Interrupt sample application at UY ma BUU0U BI n ect sam D e d G Cl V O nto Fractionate NN settings using outlet valve Fractionation type Fised outlet CO UMN usina fraction collector Fractionation destination 0 ut 1 a o in waste do not collectl Peak fractionation destination Fixed fractionation volume 2 mi 0 01 20000 00 Peak fractionation ynlume ml 0D 01 20000 00 Sqmple inlet Inject fixed sample volume Inject all Oh using QIr sensor Set maximum volume to Wosh sample pump wrth Duffer Buffer Prime s
75. ion File Open Result Navigator 2 9 2 1 2 2 Customize Customize 9 2 Curve Style and Color Edit Texts Layout Library Header CurveNames Yaxis XAxis Lurve PeakTable Ressssosssessesesssssssssssescsseseeced CU VeS J 2 Select curves to display OO1 UY 1 280 Chrom 1 Training laboration 1 O01 2 UY 2 0 Chrom 1 Training laboration 1 001 2 9 NNU 27C bb 20 003 UV 3 0_Chrom 1 Training laboration 1 001 4 Cond hrom 1 Trainina laboration 1 DO1 2 005 Cond_Chrom 1 Training laboration 1 O01 1 a 2 O DOE Eonc B_ Chrom 1 Trainina laboration 1 O01 0 pH_Chrom 1 Training laboration 1 001 O08 System flow_Chrom 1 Training laboration 1 001 7 a 9 3 Sstem pressure_Chrom 1 Training laboration 1 O01 J
76. lumn type OK Selected column tpe HiScreen Capto 5 3 OK 2 MM Pumps and pressures dei 00 19327 3 Instructions Sustem Flow Sample flow 4 Pumps and Pressures System Flow Graden Eo Pump wash ff 3 Sustem wash LI ed Flow O MT start concentrations CQuaternary gradient BufferPro pH 5 Flow rate Execute Cohi packing ow g Etro Sample flow 150 0 3 cmh Linear Flow 15 4 1 16 10 32 UNF M6 XK HiLoqd HR 1 16 male M6 female 18 3858 01 1 16 female M6 female 18 1123 94 5 16 HiScole 50 XK 50 1 16 male 5 16 female 18 1142 08 5 16 female 5 16 female 18 1173 51 HiTrqp HiPrep HiScreen
77. mss BAK yyyymmdd_hhmmss 12 6 UNICORN UNICORN 1 Administration Database Management ne Backup Restore archive Helrieve Release Dbjects Select backun file Ek Files GE Healthcare UNICORNEackup 2 Restore Name Date modiied Size IMB Unicorm_ SCHEDULED_BA amp ACKUP 20091113 03 11413 42009 3 00 21 AM 174 091 UNICORN_ MANUAL BACKUP 20090817 162 8 17 42009 4 22 34 PM 31 404 O UNICORN_ MANUAL BACKUP 20091124 132 11 24 2009 7 28 57 FM 201 404 UNICORN_ MANUAL BACKUP 20091124 203 11424 2009 8 35 20 FM 174 529 3 Restore 4
78. nologies system index asD box 08A http faq gelifesciences co jp AKTA avant 25 AKTA qvant 150 Q amp A AKTA AKTA qvqnt pn 1 1 AKTA gvant i A NP 2 et 30 11 0 0 FF ON MN i 2 XR pH 2 2 7 ME 0 PE N B Q LT NE I re VD
79. on gt Execute 2 3 20 End Js 44
80. valve Injection mak onitors raction collection km Flow path gt Column Position gt Position 2 gt Execute E Save result as A amp uto update parameter fields will be updated during method run 4 Manual Instructions Alarms Alarm pre column pressure gt High alarm Max pre column pressure Alarms Alarm delta column pressure gt High alarm Max delta column pressure Column handling Maqx pre column pressure Max delta column pressure lt Column handling gt 4 1 System control Tools Column handling 4 2 Column Handling Show by technique 4 3 Column types Column Type Parameters Maqx pre column pressure Max delta column pressure 5 Pumps qnd Pressures System Flow gt 0 2 0 5 ml min gt 6 2A
81. waste do nat collect Peak fractionation destination Fixed fractionation wolume 200 ml On 20000 00 Peak fractionation volume 200 mn1 20000nm 7 9 Equilibration Faviibraton 2 22 Heset UV monitor recommended if Ihe equilibration occurs before Ihe puniication Ei vr Use the same flow rate as in Method Settings se the same inlets as in hlethod Settings Equilibrate until the total volume is pe mMmn 0 000 25 000 etlA A v EE InletB B1 y nm BIQ0 100 v Fill the swstem with the selected buffer Delete the following condition is met Conductivity greater than mS cm 0 00 1000 00 Accepted pH fluctuation DOD 14 00 Accepted UY fuctuation 1 mau 0 00 E000 00 Accepted conductivity fluctuation D10 ms cm I0 00 300 00 Siqnal stable for min 2 1000 00 Maximum equilibration ynlume 1nmm Cy 7 10 a Methods File Save Save As nediet Cesedty
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