ウリ類退緑黄化ウイルス検出キット
Contents
1. TEL 076 451 6548 TEL 03 5977 5573 FAX 076 451 6547 FAX 03 597 5574 E mail suppot nippongene analysis com E mail feast egenome csJp fujitsu com URL http nippongene analysis com URL http genome e mpJp Copyright 2012 NIPPON GENE CO LTD All Rights Reserved2. A CCYV CCYV CCYV B 1 C EDTA
3. 4 5 CCYV 6 MSDS
4. UV SD oo UV 240 260 nm 350 370 nm a 320 nm UV UV 60 RNA
5. D A A A 240 260 nm 350 370 nm UV 320 nm
6. gt 5 10 RNA gt RNA 1 1 CCYV CCYV CCYV 20C 1 x 3
7. A B C CCYV 16 6 1 2008 62 8 424 2 2009 75 109 3 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA uc e c Acids Res 28 12 e63 4 2009
8. 23 0 gt RNA 23 0 RNA gt RNA 1 RNA lt gt RNA 2 0 20 0 ul
9. amp amp ISO 13485 2003 http www nippongenematerial com 2 24 NE0081 1 24 CCYV 20C EE EE CE 20C 50ow 6 4 20 COYV EE 4 C 20C
10. e 1 5 2 0 ml 1 5 ml 2 0 ml 0 2 ml 121 C 40 UV 1 5 2 0 ml 240 260 nm 350 370 nm 7 5 000 15 000 rpm lt gt RNA NE0051
11. 1 5 ml 2 0 ml 1 x 3 lt gt CCYV 21 0 pl 189 0 ul 525 0 ul 25 0 ul 23 0 ul 207 0 ul 575 0 ul 1 1 CCYV 1 2 lt gt
12. CCYV CCYV CCYV RNA gt RNA CCYV 11 1 lt gt lt RNA gt I 1 lI lt RNA gt 3 CCYVI I I lt CCYV gt I 1 HI
13. 2 RI 2 3 ee 3 4 oe 5 5 lt ooeeeeeeeoeoo 7 eee 1 1 RNA 5 0 16 6 PR 17 7 PE PPP FPP i 18 CCYV LAMP 1
14. CCYV 1 1 gt iD 10 000 ppm 1
15. CCYV CCYV CCYV LAMP
16. RNA lt gt RNA 6 mm 5 1 5 ml 1 ml RNA 5 000 rpm 30 25 1 5 ml CCYV 75 ul 5 000 15 000 rpm 1 CCYV RNA CCYV 300 uu CCYV 300 uu 50 ui RNA RNA RNA RNA CCYV CCYV
17. CCYV DNA DNA cDNA DNA CCYV RNA CCYV 2007 CCYV 30
18. LAMP Cucurbit chlorotic yellows virus CCYV LAMP DNA cDNA DNA RT LAMP CCYV RNA CCYV CCYV CCYV RNA CCYV CCYV 63 C 60 CCYV LAMP CCYV RNA
19. 52 72 5 Okuda M Okazaki S Yamasaki S Okuda S Sugiyama M 2010 Host Range and Complete Genome Sequence of Cucurbt chlorotic yellows virus a New Member of the Genus CriniviruSs Phytopathology 100 560 6 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 1 7 7 CCYV 2 0 25 0 ul 1 DNA 63 C 60 CCYV CCYV 2 0 ul 1x 10 18 19 20 DD 2012 11 NIPPON GENE
20. http nippongene analysis com 7 8 LAMP LAMP 9 e Genome Order e Genome Order http genome e mpp 1 5 1 LAMP Loop mediated lsothermal Amplification
21. RNA UV gt RNA RNA 15 5
22. CCYV Q B LAMP Loop mediated Isothermal Amplification LAMP DNA DNA RNA cDNA DNA RT LAMP LAMP Eiken GENOME SITE http Iloopamp eiken cojp
23. ii 70 iv 1 v 10 RNA lt gt gt IRNA gt RNA gt RNA 1
24. 4 11 1 RNA i 5 1 5 ml 6mm ii 1 ml 5 000 rpm 30 iv 25 ul v CCYV 75 ul Vi 5 000 15 000 rpm 1 vii CCYV CCYV 300 ul Vii CCYV CCYV 300 ul ix CCYV 50 ul RNA i 5 ul ii 10 A EN Gy ieom COYV ul 9 23l 207l
25. DNA CCYV CCYV RNA
26. 0 2 1 lt 3 LAMP DNA
27. RNA 1 MSDS 3 63 C 0 5 10 ul 10 100 200 1 000 ul
28. 25 24 24 6 CCYV CCYV 20 C 20 C CCYV CCYV
29. Cucurbit chlorotic yellows Virus Detection Kit VersIion 4 0 0 NE0081 DD version 4 0 0 1 LAMP 2 2 6 3
30. ii ii RNA 20 0 ul RNA RNA 20 0 ul CCYV 2 0 20 0 gt 20 0 ii iii iv RNA 2 2
31. 3 0 uu 20 0 uu RNA 20 0 ul CCYV 2 0 20 0 13 lt gt gt 1 x3 lt 2 63C 60 14 60 80 C 2
32. 575 1 1 23 ul RNA 5 RNA 2 ul 20 ul 63 C 60 80 C 2 UV PE oo 6 RNA RNA gt gt gt RNA RNA 1
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