NanoDrop 2000/2000c 分光光度計
Contents
1. Rate Rate2. Starna UV lt 340 nm UV UV im 220 nm UV
3. NanoDrop 2000 2000c e Home Classic s My Data
4. Sample ID 28 Sample ID On Off E gt gt amp
5. NanoDrop PR 1 PR 1 www nanodrop com 3 17 Protein A280 Add to report Overlay spectra O Small sample volume O Use cuvette 10mm Absorbance 15 10 ns na 3 Sample ID Pedestal Tee A280 10 mm path 6 090 260 280 0 63 Baseline correction 340 nm Z0 30 30 320 D 3 2 340nm 0 000Abs 8 10 mm
6. NanoDrop PR 1 PR 1 www nanodrop com 3 29 3 Protein Bradford E Protein Lowry C Documents and Settings All UsersWDocumentsVThermo NanoDrop2000 2009_02_25 Protein Lowry twbk Fie Help D Load your sample and press the measure button Measure Prnt Blank ReBlank Data Standard Curve Vaidcuve E 9 Samples Pedestal v Add to report EUR i 050 119 Sample ID 10 Verlay spectra gis Concentration mg ml 1 010 i Absorbance at
7. Page Setup 2 9 2 s Preference Support Dymo Label printer Keyboard Shortcut 2 NanoDrop Classic NanoDrop Classic Standard Exit Ctrl q Alt F4 Help Ctrl m Ctrl h F1 Blank F3 F3
8. Manually entered factor extinction coefficient Method Editor Information Standard Curve Standard Single Point Standard Curve Standard Standard Standard Curve with two wavelength 2 Advanced Standard Curve
9. 190 840 nm NanoDrop 2000 2000c 200 NanoDrop 2000 2000c Pedestal 0 5 HL 0 05 mm 1 mm 2048 CCD 190 840 nm 1nm 1 8 nm FWHM at Hg 253 7 nm 0 002 1 mm path 296 at 0 76 at 257 nm 0 02 300 10 mm Path 2 ng uL dsDNA 15 000 ng uL dsDNA lt 5 WxD 14 cm x 20 cm 2 0 kg SUS303 12 VDC 12 18 W 30 W Windows XP Vista 32 bit NanoDrop 2000c Cuvette
10. Note Add to Report Report Report Report 3 e Report Configura
11. 2009 Thermo Fisher Scientific Inc All rights reserved Microsoft Windows Windows NT Excel Microsoft Corporation Adobe Acrobat Adobe Systems Incorporated Thermo Fisher Scientific NanoDrop Thermo Fisher Scientific 2009 3 Tz CEA AO a e aao NDS 1 1 r3 rdiniol rg D uua Ic EM 1 1 ANA EUER orudeh etit ecu ette et itiet cde otii Pedes o TASA cado abt tp asi kt MSIES cile oM ise a cde cat Sagas
12. Note Standard 1 dug Protein Pierce 660 nm e e Reference Standard Dye e Standards Standard Blank pH e Standard Standard
13. 10 mm 3 0 gt 150 ng uL dsDNA Small sample volume 3 3 3 Nucleic Acid Nucleic Acid IP File Help Measure Print Blank Re Blank E Add to report O Overlay spectra O Small sample volume Jw Load your sample and press the measure button Sample ID dsDNA Pedestal Tve DHAN 5000 cox BENE oo lt A260 10 mm path 9 691 A280 10 mm path 5 045 200 280 1 92 2601230 2 08 O Use cuvette E Baseline correction 340 nm 337nm 0 000Abs g 10mm Cuvette
14. File Use current settings as default Report Add to Report Add to Report Overlay Spectra Blank Blank Blank pH Pedestal 1 2 uL Blank Blank Cuvette
15. 1 2 List names from Organization Users and groups 3 Users and groups Add Users e List names from e Organ
16. Add to Report Overlay Spectra Blank Blank Blank pH Pedestal 1 2 uL Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note
17. NanoDrop 2000c Eppendorf Uvette amp Cuvette 2 mm 8 5 mm Cuvette 1 2 F9 e PC 1
18. Standard 1 HL 2 HL Cuvette 2mm 8 5 mm
19. 2 uL Cuvette 2mm 8 5 mm Pedestal Cuvette NanoDrop 2000 2000c 600 nm 400 nm
20. Standard 1 EIo Bradford e e Reference Standard Dye e Standard Standard Blank pH e Standard Standard 3 35 nn 6 3 36 3 Protein Bradford Wavelength verification
21. mg mL 340 nm Baseline Correction File Use current settings as default Report Add to Report Add to Report Overlay Spectra Blank
22. 3 rf Load your sample and press the measure button i Print Bk Data SG Valid Curve Samples Pedestal E Add to report 0 50 11000 ug m Sample ID C Overlay spectra 045 Concentration pg ml 943 800 Absorbance at 660 nm 0 115 Use cuvette 040 GS O Standards 035 030 eatt ilg 13 o5 E f 020 015 awf ms La anj z e 0 00 005 550 560 800 S20 40 55D 700 720 Wavelength Inm 650nm 0 108Abs L Sample ID Protein Conc Unt AB60 12 125 ug ml 161 711 ugiml 0 018 13 125 ug ml 154 154 ugiml 10 017 14 125 ug ml 119 618 ugiml 0013 15 125ugml 117 201 ueiml 0 012 16 1000ug ml 956 066 ugimi 0117 1 mm Pedestal Cuvette 2000c Y s Data e Standard Curve R2 Interpolation
23. Standard Standard Blank Blank Blank pH Pedestal 2 HL Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note
24. Pedestal Blank Sample ID 7 Blank Measure Note 3 3 15 3 Cuvette 3 16 3 Protein A280 Protein A280 Trp Tyr Cys Cys
25. Measure Delete Kinetic Delete 2 Group list Classic Method list 5 1 5 Kinetics Kinetics Editor New 2 Previous Next Finish Cancel
26. Overlay Spectra Overlay Spectra Sample ID Sample ID Overlay Spectra o Small sample volume Nucleic Acid Protein A280 10 mm 3 0 gt 150 ng uL dsDNA 0 5 uL Use Cuvette NanoDrop 2000c Pathlength 10 5 2 1 mm Stir speed 1 10
27. e Diagnostics Intensity Check Calibration Check Diagnostics and Troubleshooting e Options 4 e Measure s Reports 3 Repot Configuration Print e Oligo Calc Nucleic Acid Micro Array
28. DER k Method Editor ClassicVCy31 Fie Help rl V Name amp Type Measurement Correction Additional Measurements Instrument Settings New Save Measure Delete Formula table Optional Group list Name Formula Classic A550 A 550 Cy3 formula A 550 150000 1000000 PathO CE Lysozyme A 280 10 28 4 PathQ Cy3 v iz Copy to another group Path returns the pathlength of the sample in centimeters A nm returns the absorbance of the sample at the specified wavelength Add from predefined formula Build formula Lysozyme A 280 10 26 4 Path mg ml Report Lysozyme 280 nm 10 26 4 cm Note E196 0 196 10 mg mL Cuvette Pedestal 0 1 4 3 LEF Lysozyme mg mL
29. e Sample ID Sample ID Sample ID s Type dsDNA DNA 50 RNA RNA 40 ssDNA ssDNA 33 Oligo DNA Oligo RNA DNA 50 Custum 15 150 o e Conc 260 nm e A260 260 nm 10 mm e A280 280 nm 10 mm
30. Standard 1 HL 2 HL Cuvette 2mm 8 5 mm
31. My Data The workbook 2009 02 13 Protein BCA uses a standard curve Would you like to 9 Start a new workbook O Start a new workbook using the concentration values from this workbook Start a new workbook using the standard curve from this workbook Append new measurements to this workbook o 2 8 2 Reprocess Report Reprocess Baseline Correction Wavelength Concentration Unit Sample Typ
32. Pierce 660 nm 15 1 Pedestal 60 HL uL Standard Standard BSA
33. Protein A280 1 Abs 1 mg mL e Ext Coeff E196 L gm cm Other protein E196 e 8 1000 M W KDa Other protein E amp MW e 1000 M W kDa e Conc 280 nm mg mL e Dye 1 2 Dye Dye 1 Cy3 IZ Dye 2 Cy5 Abs Dye 1 mm Abs Dye 1 mm uM Dye
34. Overlay Spectra Blank dHzO Blank Pedestal 2 uL dH O Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal Standard Standard Blank Standard
35. Rate Add Remove e Instrument settings UV Visible UV Vis Custom Kinetic Kinetic Save Kinetic Measure Note
36. Pedestal Kinetic PRISES Default kinetics method desenes Deolota Method name Description Result label Rate Time units Seconds h Measure stage Wavelengths to Monitor Stage Stage Cum Item Wavelength Stage Interval Duration Time 1 sec sec sec 1 lt Previous Next gt Finish Cancel Kinetics Editor Kinetics Editor New Save Save Note Method list Copy to another group Classic Measure Kinetic Kinetic
37. 3 4 3 Note Baseline correction 3 5 3 Nucleic Acid 1 Nucleic Acid Wavelength verification OK Type DNA 50 Conc ng uL 340 nm Baseline Correction
38. Overlay Spectra Blank 3 9 3 Blank Blank pH Pedestal 1 2 uL Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal
39. Y e Sample ID Sample ID Sample ID 3 8 3 Type dsDNA DNA 50 RNA RNA 40 ssDNA ssDNA 33 Oligo DNA Oligo RNA DNA 50 Custum 15 150 Conc 260 nm A260 260 nm
40. e Units e Advanced Method Editor 1 Method Editor File Help RC U M New Save Measure Delete Method name Default Method Group list en Description Optional Classic 4 Method list Default Method 1 Method type Manually entered factor extinction coefficient iz Copy to another group Standard curve Single point standard curve Standard curve with two wavelengths O Advanced standard curve Method Editor s New Save
41. 10 uM Cation Molarity 10 mM 96 Formamide 0 0096 ER Melting Point Salt Adjusted Nearest Neighbor 3 7 3 Micro Array DNA NanoDrop 2000 2000c DNA
42. Spectra New Workbook twbk NanoDrop 2000 2000c ND Legacy tsv Report Excel 1 Note Kinetic Spectra New Workbook twbk ND Legacy tsv PC xml Excel Excel
43. Autosave Autosave Autosave NanoDrop 2000 2000c Spectra New Workbook twbk My Data Autosave Options Options 4 e Applications
44. Lowry Folin Ciocalteu 650 nm 405 nm Standard 20 HL 100 uL Lowry NanoDrop 2000 2000c Lowry 0 20 mg mL 4 mg mL o Standard Standard BSA NanoDrop 2000 2000c
45. Save Method list Copy to another group Classic e Measure 4 1 4 Method Editor Measure e Delete Method list 2 e Group list Classic o e Method list Name amp Type
46. Cuvette 3 44 4 Method Editor 4 Method Editor Method Editor Method Editor Editor Options Method Editor Editor Options 3 Method Editor e Formula Formula Method Editor Note Reset
47. Note Standard 1 rica BCA e e Reference Standard Dye e Standards Standard Blank pH e Standard Standard 3 27 nn 6 3 28 3
48. 2mm 8 5 mm NanoDrop PR 1 PR 1 www nanodrop com 3 33 3 Protein Bradford E Protein Bradford C Documents and Settings All UsersDocumentsVThermo NanoDrop2000 2009_02_25 Protein Bradford twbk Load your
49. Measure Standards standard curve is needed for this application Absorbance data For standards can be either measured nr entered manually Click Yes to measure absorbance data or No to manually enter the absorbance values B 3 73 Standard Note 1 Ex Protein Pierce 660 nm Fie Hep dr Load your standard and press the measure button pioen Pik Bank Data Standard Curve Vaid Curve O Samples Pedestal 7 Add to report 02 E 9 Overlay spectra 024 Use cuvette d pt 020 um 9 Standards 018 pt Measure Conc AvgAbs Bes 016 T Reference 0000 3 0 001 Heat to 37 C ou Ps Standard 1 25000 3 0 003 i Standard
50. 1 Lowry e e Reference Standard Dye e Standards Standard Blank pH e Standard Standard 3 31 nn 6 3 32 3 Protein Lowry Wavelength verification OK Standard
51. ng uL Dye 1 Dye 2 Dye Dye 1 Cy3 Dye 2 Cy5 Dye Dye 2 None 340 nm Analysis correction File Use current settings as default Report Add to Report Add to Report
52. Cuvette Blank 30 Blank 30 Blank Blank 3 1 3 Sample Type 5 sls SD ng uL CV 96 i 2 ng uL pedestal X 15 000 ng uL 2 100 ng uL 2 ng uL Nucleic A pee auus 0 4 ng uL cuvette dsDNA gt 100 ng uL 296 2 ng uL pedestal 2 100 ng uL 2 ng uL 7 L dsDNA Microamay 0 4 ng uL cuvette SD DS gt 100 ng uL 2 aM SA BSA dl 0 10 10 mg mL 0 10 mg mL Protein A280 pedestal 400 TAS a ong BSA Proteins
53. 3 25 3 NanoDrop PR 1 PR 1 www nanodrop com BCA Protein OS ac Load your blank solution and press the blank button 9 Samples Pedestal Sample ID SAMPLE 1 Concentration mgimk 0 560 Absorbance at 562 nm 0094 Standards Measure Conc Avg Abs 0 003 0043 0 126 0247 OC 0 Protein Conc Unt A562 Baseline Corr Abs Baseine Corr nm integration ms ef 560 mo xi 5 r 1 mm Pedestal
54. Re Blank Blank Re Blank 4 Method Editor Kinetic Editor New New Method Wizard Save Measure Delete File New Workbook Close Workbook and go Home Home
55. 9 Blank Sample ID Blank Measure Note Cuvette Oligo Calc Oligo Calc Oligo Calc 2 e Oligo Calc e Melting Points DNA DNA Oligo Calc 1
56. e Instrument settings Auto Pathlength Pedestal Auto Pathlength Auto Pathlength 1 mm 1 25 190 nm 219 nm 1 mm 1 0 Auto Pathlength 1 mm e Standard Standard curve Standard Standard Linear Linear through zer
57. o Cell Culture 1 Cell Culture Wavelength verification OK 2 Report Add to Report Add to Report 3 Overlay Spectra 3 43 3 4 Blank Blank Blank pH Pedestal 2 HL Blank
58. Standard Replicate s Standard Standard 1 Standard e Standard e Note Standard
59. Lysozyme 19 10 mg ml 280 nm Lyszyme 26 4 Type Otherproten E amp MW e 1000 0 00 M cm1 MW WW Da 000 Type SA 1 10 mg ml L gm1cm 1 Ext Coeff E 1 LUgm cm 000 e Ext Coeff E196 L gm cm Other Protein E196 3 18 3 8 1000 M W KDa Other protein E amp MW e 1000 M W kDa Conc 280 nm mg mL A280 10m
60. UV VIS NanoDrop 2000 2000c DNA dsDNA 15 000 ng uL UV VIS A280 BCA Lowry Pierce 660 nm Assay Kinetic 1 Add to Report 2 Blank Pedestal 1 2 uL
61. Kinetic 1 Autosave Kinetic Home Kinetics Editor Kinetic Option Home 5 2 5 Kinetics ea e pg in Blan Sample ID SN00062 B gal RT View Method Setting Use cuvette Tmel Tme2 Factor Rae Bared 24000 72000 296 00020 0 999988 Pathlength 10 mm i NN 240 00 720 00 2 96 0 0017 0 999988 Stir speed Off Y O Heat to 37 C 10mm Absorbance 19 o z 5 m a 5 E Es E E eo Wavelength nm Kinetics 2
62. Home Nucleic Acid ng cm uL c A b c ng uL A 8 ng cmuL b cm e Double stranded DNA 50 ng cm uL s Single stranded DNA 33 ng cm uL e RNA 40 ng cm uL Pedestal NanoDrop 2000 2000c Note Report Pedestal Cuvette Nucleic Acid 1 0 cm 10 0 mm NanoDrop 2000 2000c dsDNA 15 000 ng uL
63. Base Sequence A C G T ERU U Base Sequence amp A C G T U Clear 2 DNA Mono phosphate RNA Mono lt Tri phosphate Double Stranded Nucleic Acid DNA 5 Modification Oligo Calc e Molecular Weight e Extinction Coefficient 260 nm
64. E1 Proteins amp Labels Fle Help s D amp Load your sample and press the measure button Measure Print Blank Re Blank 5 Sample ID IgG Alexa 555 Pedestal v Add to report Type lgG Overlay spectra 115 110 Use cuvette 105 Conc 0 33 mg ml wv v Analysis correction 340 nm A280 10 mm path 0 576 Heat to 37 C 90 Dye 1 85 Alexa Fluor 555 0 328 Abs 22 uM x 75 Dye 2 None S 70 0 000 Abs 0 0 uM Sloping dye correction 400 750 nm 10mm Absorbance D DIS 2 750nm 0 000Abs 10mm Cuvette e Sample ID Sample ID Sample ID s Type 6 Type
65. Of TH Heat to 377C 37 5 C 37 C 1 10 Note Pedestal 2 5 2 Group Classic NanoDrop s s
66. W Meses PN Beh Beth a T ae Js Em EE e Autoscale all samples Y Y e Full Display all samples e Set Scale Y e Sample labels UV Vis e Sample legend Sample ID 2 6 2 My Data My Data Navigator
67. Microsoft Windows XP Vista 32 bit 1 5 GHz CD ROM 1GB RAM Vista 2 GB 40 MB USB USB USB PC PC Administrator 1 USB 2 CD Run xMetup OK
68. OK Standard Reference 7 Standard Reference Standard Note 2 Standard 1 Reference 1 Standard 2 Standard Report Add to Report Add to Report File Use current settings as default
69. e 7 Standard Replicate Standard e Standard 3 39 3 Standard Replicate s Standard Standard 1 Standard e Standard s
70. 0 150 A Bradford Standard 3 NanoDrop 2000c Cuvette o 1 HL 2 HL Cuvette
71. Installation Qualification IQ IQ Thermo Software IQ 1 1 2 gt Thermo gt Thermo Software IQ 3 2 1 2 2 4 Help Thermo IQ User Guide PDF 2 2 PC USB 12 V 5 W
72. Blank Blank pH Pedestal 1 2 uL Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal Blank Sample ID 7 Blank
73. s Measurement 1 mm Pedestal Cuvette 2000c Y e Correction Baseline correction 3 None Single point Sloping baseline Analysis correction Baseline correction e Additional Measurement
74. OK 750 nm Baseline Correction nm Add wavelength s 40 READ Enter Add Wavelength File Use current settings as default Report Add to Report
75. EA 4 1 Methed Editor 4 1 etus cemere nani A Act AA NARO NA heran ER ct NR 4 1 OO 4 2 4 6 5 A Kifietics she EEEE AE LLLI AI A ENEE EEEE derent 5 1 Kin tics Editor OS BE ce ceto tee a tei ede a eden i te En Des 5 1 Re 5 1 ZUM a tud tamm e a b equ D p Marie ice rod M em adorti su EU Ando cq 5 2 SR 5 3 Ratet 5 3 KiIhGtiG X AN 5 4 RS ee i e MM DC RT MERE EUN WE RNN RM NIR d 7 1 Thermo Scientific NanoDrop 2000 2000c 0 5 2uL NanoDrop 2000c NanoDrop
76. Cuvette 2000c Y s Data e Standard Curve R2 Interpolation Linear Polynomials Linear e Valid Invalid Currve Standard Invalid Curve Valid Curve amp amp Note Standard e Samples Valid Curve Sample ID Sample ID
77. 0 2 pL FH 2 HL Pedestal 1 w a 4 2 3 1 1000 yt l eL A JQ C S ECC Lue en 1 Cuvette NanoDrop 2000c 48 mm 10 mm
78. A280 10mm path 280 nm 10 mm Note A280 750 nm 280 nm A280 750 nm Dye Analysis correction A280 3 22 3 Analysis correction Analysis correction Sloping Dye Correction 400 nm 750 nm Dye Protein amp Labels 1 10
79. 0 0096 Melting Point e Salt Adjusted e Nearest Neighbor 3 12 3 UV Vis UV VIS NanoDrop 2000 2000c 190 nm 840 nm 40 Report NanoDrop 2000 2000c Auto Pathlength 10 mm 300 A UV Vis Hle Hep TR
80. 2mm 8 5 mm Note Pedestal Blank Sample ID 7 Blank Measure Note 3 23 3 Cuvette 3 24 3
81. Standard Standard Samples Sample ID Pedestal 2 uL Cuvette Measure Standard Blank Note Cuvette 3 Protein Pierce 660 nm Thermo Scientific Pierce 660 nm Protein Assay
82. Rate 1 Add 6 2 amp ID Note Rate Lock to curve 5 3 5 Kinetics Rate Rate
83. Oligo Calc Oligo Calc Oligo Calc 2 Oligo Calc Melting Points DNA DNA Oligo Calc 1 3 6 Base Sequence 3 A C G T U Base Sequence amp A C G T U Clear DNA Mono phosphate RNA Mono lt
84. Measure Note 3 19 3 20 3 Cuvette 3 Proteins amp Labels A280 nm metalloprotein NanoDrop 2000 2000c 100 pmols uL Cy3 20 mg mL BSA
85. 2000c Cuvette 0 596 1 10 Note 3 42 3 Cell Culture Sample ID Cell Cultures Sampid Pedestal Add to report i C Overlay spectra 15 Absorbance correction 0 000 Use cuvette 5 600 nm Abs 0 085 120 115 User cursor nm 280 110 10 User cursor Abs 0 961 1 0
86. 3 10 UN IS BERE PETITES OE CHARIOT EET ED TARTE AMETE A a DIE EUM 3 13 Lor LEE TIME MM 3 13 Etna RR LED 3 13 UVVis 3 14 Proteir A280 5 ote auus uiendenmuithctuim ue denimedet et e COEM Min 3 17 Dor M EET MUN 3 17 DEF ENEMIES 3 18 Protein A280 dr I PE PPAGOY y ERR 3 19 Proteins SOS 3 21 ror IL ELM MM 3 21 Dye Chromophore Edltor 2 3 eI bsp RECO noD ERE RR e RR MEE 3 21 It 3 22 Protein amp Labels ii 3 23 Protein B OA Ns 3 25 Do Deco 3 25 CHI 3 26 BGA EE 3 27 Protein LOWY Em 3 29 TEN 3 30 Lowry 3 31 Protein Bradford exuere ue cA 1 SG SR 3 33 3 33 EE N E RE E I E EE EE N E E AE E E E 3 34 Bradford e a E E E AA E eth E A A lt i lt 3 35 Pretein Pieree 660 NMm a a A NES 3 37 EE E AEO ETE 3 37 EE ATN NEE E A ENA ASEA E RCM E E A RE NER 3 38 Protein Pierce 660 nm kkk 3 40 Cell Culture SI ET SS S ASEEEEEE I 3 42 3 42 Re E ERREUR R M 3 43 Cell Gulture Fa 6 lE o ode dome denda cUm dona du A don udi 3 43 4 Method Editor 0 ino e o eee eoe ette be ee Doro ve debere deve ene eoe a Uer eade cue 4 1 Editor Options C
87. Add Wavelength Add Wavelength Clear Wavelength Add Wavelength 1 Clear All Add Wavelength UV Vis Sample labels ON OFF Sample labels UV Vis 1 3 14 UVNis Wavelength verification
88. Reference 7 Standard Reference Standard Note 2 Standard 1 Reference 1 Standard 2 Standard Report Add to Report Add to Report File Use current settings as default Overlay Spectra Blank
89. Use current settings as default 4 Overlay Spectra 5 Blank Dye Blank Reference Pedestal 2 nuL Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal 3 40 6
90. 1 Protein Pierce 660 nm Wavelength verification OK 2 Standard Reference 7 Standard Reference Standard Note 2 Standard 1 Reference 1 Standard 2 Standard 3 Report Add to Report Add to Report File
91. 3 Standard Standard Blank Standard Measure Standard Standard Samples Sample ID Pedestal HL Cuvette Measure Standard Blank Note Cuvette 3 41 3
92. Sample ID Pedestal 2 uL Cuvette Measure Standard Blank Note Cuvette 3 Protein Bradford Bradford UV 280 nm Bradford
93. Bradford 595 nm 595 nm 750 nm Bradford 2 es 0 1 mg mL 8 0 mg mL BSA 50 1 0 01 1 mg mL Pedestal Bradford 200uL uL 15 hg mL 125 ug mL BSA 1 1 Pedestal
94. Kinetic Kinetic Overlay Spectra Sample ID Note Sample ID
95. Standard Invalid Curve Valid Curve Note Standard e Samples Valid Curve Sample ID Sample ID e Standard Standard e Concentration mg mL mg mL e Absorbance at 650 nm 650 nm 3 30 Lowry Lowry F Protein Lowry C Documents and Settings All Users DocumentsYThermoWanoDrop200012009 02 25 Protein Lowry twbk 3 F
96. x CD Rom 3 USB Found New Hardware Wizard Windows XP SP2 No not this time Found New Hardware 7 Welcome to the Found New A Hardware Wizard Windows XP i SP2 Vista Diagnostics and Troubleshooting 44L Web www nanodrop com Thermo Software IQ Thermo Software IQ NanoDrop 200072000c
97. 20 1 Pedestal BCA 80 pL 4 pL s 0 10 mg mL 0 20 mg mL BSA 1 1 Pedestal 10 LL 10 uL O BCA PCR Standard Note 60C 2 Standard BSA Hete mg m Standard BSA IgG NanoDrop 2000 20000
98. Cuvette e Sample ID Sample ID Sample ID s Type 6 Type 1 Abs 1 mg mL 280 nm 1 0 A 10 mm 196 1 mg ml 1Abs imgimE Bovine Serum Albumin 1 10 mg ml BSA BSR c D 280 nm 6 7 IgG 1 10 mg ml IgG 280 nm 13 7 ue
99. Fle Help SS ES J An Data Start a new workbook or select a stored workbook to load To sort list click on a column header W amp G Desktop New Open m f Favortes Application Filename Sample mode Date Samples Active workbook folder Proteins amp Labels 2009 02 17 Proteins amp Labels twbk m 2 17 2009 12 25 47 PM j My default workbook folder 2007 Protein A280 2009 02 17 Protein A280 twbk 2 17 2009 12 25 07 PM 1 System default workbook folder 2 med rs Micro Aray 2009 02 17 Micro Array twbk 2 17 2009 12 22 08 PM 2 My Documents folder E LabVIEW Data Default Method 2009 02 17 Classic Default Method twbk Pedestal 2 17 2009 8 07 52 AM 2 Autosave folder d ed Default kinetics method 2009 02 17 Classic Default kinetics method twbk Cuvette 2 17 2009 7 43 40 AM a eA Mv Meetina Files My Data 2 New Open New Open 4
100. PC twbk Pedestal DNA RNA 1 HL 2 HL 1 uL 2 uL Bradford BCA Lowry Pierce 660 nm 2 uL 2 HL 1 2 HL
101. ng cm uL 3 10 3 Concentration Factor Number of bases GC 3 11 3 DNA 1 Oligo Calc Melting Points Base Sequence 2 Oligo Molarity 10 uM Cation Molarity 10 mM 96 Formamide
102. Measure Standard Standard Samples Sample ID Pedestal 2 uL Cuvette Measure Standard Blank Note Cuvette 3 Protein Lowry Lowry Lowry Lowry
103. 280 nm BCA Pierce 660 nm NanoDrop 2000 20006 Protein A280 UV 280 nm A280 mg ml Nucleic Acid Protein A280 10 mm 1 cm NanoDrop 200072000c 400 mg mL BSA
104. Standard e Standard Standard Replicate s Standard Standard 1 Standard e Standard e Note
105. Blank NanoDrop 2000 2000c Blank Blank Intensity mo Absorbance log Intensity Blank A E b co A 8 liter mol cm b cm c Blank pH Pedestal Blank Blank
106. 10 uL 10 uL Bradford PCR Standard Bradford Standard BSA Note NanoDrop 2000 2000c Standard 1 0 mm Bradford Coomassie pH 595 nm
107. Pedestal Standard Samples Sample ID Pedestal 1 2 uL Cuvette Measure Standard Blank Note Cuvette 5 Kinetics 5 Kinetics NanoDrop 2000c Kinetic Kinetics Editor Kinetic
108. 1 HL 2yL Cuvette 2mm 8 5 mm NanoDrop PR 1 PR 1 www nanodrop com 3 37 Protein Pierce 660 nm
109. Blank Dye Micro Array Proteins amp Labels Dye Dye Chromophore Editor Dye Dye Dye Dye list Show Dye Coeff Umole cm CN 260nm nm 1 280nm Wavelength Correction Correction None 0 0 0 Cy3 1 5E 5 550 0 05 S Cy5 Alexa Fluor 488 2 5E 5 7 1E 4 495 650 0 05 0 11 Alexa Fluor 546 1 04E 5 0 12 JEJE Alexa Fluor 555 Alexa Fluor 594 1 5E 5 7 3E 4 555 590 0 08 0 56 Alexa Fluor 647 2 39E 5 0 03 Alexa Fluor 660 Cy3 5 1 32E 5 1 5E 5 0 10 0 24 Cy5 5 2 5E 5 0 18 Y s b 2 2
110. Delete Dye Dye 1 Cy3 Dye 2 Cy5 Micro Array E Micro Array File Help di Load your sample and press the measure button Measure Print Blank Re Blnk 1 05 dssDNA Sample ID EE Pedestal 2 Add to report 1 00 Type ssDNA s300 Overlay spectra 035 Conc 1155 ngnl 0 90 Use cuvette v Analysis correction 340 nm 0 85 A260 0 382 0 80 dz 260 280 1 68 Heat tn 37 C Dye 1 z 0 70 Cy5 s 065 7 3 u B 1 057 Abs 42 3 pmollul v 060 Dye 2 B 055 None x m 0 000 Abs 0 0 pmollul v E 45 0 40 0 35 0 30 025 0 20 015 0 10 r 0 05 FR niti 300 400 500 600 700 Wavelength nm cc 686nm 0 051Abs A 1 mm Pedestal Cuvette 2000c
111. Blank Blank Blank 1 2 mm 8 5 mm Note Sample ID Blank Measure 5 Kinetics 7 Note Kinetic Sample ID
112. File Use current settings as default Report Add to Report Add to Report Overlay Spectra Sloping Dye Correction Blank Blank Blank pH Pedestal 2 uL dHzO Blank Cuvette 2000c Use Cuvette
113. Note Autosave Technical Support Help Ctrl m Ctrl l h F1 Help Preferences Help About 2 4 Add to Report Autosave Add to Report Add to Report Overlay spectra
114. Overlay Spectra Blank dHzO Blank Pedestal 2 uL dH O Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal Standard Standard Blank Standard Measure
115. Add Classic 2 List of Method Options Home List of Method 9 1 Delete group Clear App Buttons e Report Master Page
116. Dye Dye Chromophore Editor Show Dye correction factor Dye 280 nm corrections Dye FO Delete Delete Dye Dye 1 Cy3 Dye 2 Cy5 3 21 3 Proteins amp Labels
117. Vector Name Rate Time 1 Rate Time 2 Rate Factor Rate 2 Rate R Squared Rate Rate Rate Rate Rate vector Remove Rate Rate Rate Rate Color Line Thickness v Lock to Curve Delete
118. 1 HL 0 2 pmol NanoDrop 2000 2000c 100 pmols uL Cy3 750 ng uL DNA Dye Chromophore Editor NanoDrop 2000 2000c Dye Dye Chromophore Editor Dye Dye Dye Chromophore Editor Show Dye correction factor Dye 260 nm corrections Dye FO Delete
119. 190 nm 219 nm 1 mm 1 0 Auto Pathlength UV Vis Auto Pathlength 1 mm 1 mm 3 13 3 Baseline correction 750 nm Note Baseline correction Add Wavelength
120. Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal 5 Blank Sample ID 7 Blank Measure Note
121. Kinetic 1 Report Configuration Sample ID Report Print Method Info Sample ID 5 5 5 8 J tt 130 0021 1 8 9 A amp Y TEL 03 5625 9711 FAX 03 3634 6333 532 0003 5 1 3 702 TEL 06 6394 1300 E mail webmaster amp scrum net co jp Internet www scrum net co jp
122. Blank Blank 1 Blank Blank Blank Measure 0 04 A 10 mm 4 10 mm 0 04 A Blank 30 Blank 30 Blank
123. dH Blank Pedestal 2 uL dHzO Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal Standard Standard Blank Standard Measure Standard Standard Samples
124. Dye 1 Cy3 Dye 2 Cy5 Abs 1 mm Dye pmol uL Dye Analysis correction Analysis correction 340 nm Note 750 nm Dye 400 nm 750 nm Micro Array 1 Micro Array Wavelength verification OK Type DNA 33 Conc
125. Load your sample and press the measure button re int Bl l Sample ID test Pedestal v Add to report Overlay spectra RUDDERIYDE 3d v Baseline correction 750 nm Use cuvette Add Wavelength 0 009 Abs i 10 mm 14 Clear Wavelength Clear All 13 nm Add wavelength s Abs Heat to 37 C 350 12 1mm Absorbance o 200 250 300 350 400 450 500 550 e00 650 700 750 800 Wavelength nm M 750nm 0 000Abs 1 mm Pedestal Cuvette 2000c Y e Sample ID Sample ID Sample ID e Auto Pathlength 220 nm 840 nm 1 mm 1 25
126. o 1 uL 2 HL NanoDrop PR 1 PR 1 www nanodrop com Dye Chromophore Editor NanoDrop 2000 2000c Dye Dye Chromophore Editor Dye
127. e 260 280 260 nm 280 nm DNA RNA DNA 1 8 RNA 2 0 280 nm Diagnostics and Troubleshooting 260 280 WHE e 260 230 260 nm 230 nm 260 230 260 280 1 8 2 2 e Baseline correction 340 nm
128. Blank Blank Cuvette 2000c Use Cuvette 2mm 8 5 mm Note Pedestal 3 Blank Sample ID 1 HL Cuvette Measure Note
129. Protein BCA Wavelength verification OK Standard Reference 7 Standard Reference Standard Note 2 Standard 1 Reference 1 Standard 2 Standard Report Add to Report Add to Report File Use current settings as default
130. Protein BCA BCA Bicinchoninic Acid UV 280 nm A280 BCA Bicinchoninic Acid BCA Cu 2 Cu 1 2 BCA 1 Cu BCA 562 nm 750 nm BCA CuSO4 Thermo Fisher Scientific BCA 2 0 20 mg mL 8 0 mg mL BSA
131. Proteins amp Labels Wavelength verification OK Type 1 Abs 1 mg mL Conc mg mL Dye 1 Dye 2 Dye Dye 1 Cy3 IZ Dye 2 Cy5 Dye Dye 2 None 340 nm Analysis correction
132. 1 Standard 2 e 7 Standard Replicate Standard e Standard Standard Replicate s Standard Standard 1 Standard e
133. 10 mm 3 0 gt 4 5 mg mL BSA Small sample volume 1 HL 2 HL Cuvette 2mm 8 5 mm
134. 4 4 4 Method Editor 4 Method Editor C A 280 A 280 Min x y x y Max x y x y abs x x Ceiling x x Floor x x Round x x Sqrt x x Area x y x y Path cm A X x R x x T x x C x x Ln x x exp x e 2 718 e x Log x or log10 x x 10 Pow x y x y XP C IDocuments and Settings All Users
135. 10 mm TH Note A260 750 nm 260 nm A260 750 nm Dye Analysis correction A260 260 280 260 nm 280 nm DNA RNA DNA 1 8 RNA 2 0 280 nm Diagnostics and Troubleshooting 260 280 Dye 1 2 Dye
136. 2000c Use Cuvette 2mm 8 5 mm Note Pedestal Blank Sample ID 7 Blank Measure Note Cuvette
137. Cell Culture NanoDrop 2000 2000c Pedestal Cell Culture 1 mm 1 cm 10 mm Pedestal 1 10 NanoDrop 2000c Cell Culture 250 700 nm
138. Tri phosphate Double Stranded Nucleic Acid DNA Modification Oligo Calc Molecular Weight Extinction Coefficient 260 nm ng cm uL Concentration Factor Number of bases GC DNA 1 Oligo Calc Melting Points Base Sequence 2 Oligo Molarity
139. Color Rate Line Thickness Rate Lock to Curve Delete Rate Kinetic 5 4 1 Kinetic Kinetic Editor Measure 10 mm Heat to 370C Heat to 37 C 37 1 lt 10
140. NanoDrop 2000 2000c V1 0 Thermo Fisher Scientific
141. Nucleic Acid Micro Array Oligo Calc e Dye Chrom Editor Micro Array Proteins amp Labels Dye Dye Micro Array Proteins amp Labels Dye Chromophore Editor e Editor Options Method Editor Method Editor 4 e Measure Measure Blank 2 3 2 Print Screen Blank Carrier Liquid Blank
142. Close All Workbooks and go Home Home Print Report Use current settings as default Sample Type Unit Type baseline correction wavelength pedestal cuvette E mail current workbook E mail NanoDrop products technical support My Data Autosave NanoDrop 2000 2000c Technical Support
143. amp pedestal 20 or ds 0 10 10 mg mL 0 10 mg mL Labels 0 010 mg mL BSA cuvette 0 2 mg mL 20 1 m ELT Modified Lowry 0 2 mg mL 4 0 mg mL 296 over entire range 8 0 mg mL En 296 over entire range 0 01 mg mL over entire range 100 y 100 500 ug mL 25 ug mL Bradford 50 1 s 8000 hg mL 500 8000 ug mL 596 15 pg mL 15 50 ug mL 4 ug mL 109 HOMS 50 125 ug mL 5 50 ug ml 50 125 ug ml 50 125 pg ml 3 ug ml 15 1 gt 125 ug ml 2125 ug mL 296 Pierce 660 nm 25 pg ml 25 125 ug ml 25 125 ug mL 3 ug ml 7 5 1 gt 125 ug ml 212 ug mL 296 Cy3 Cy3 5 0 20 4 0 pmol uL Alexa Fluor 555 0 2 100 0 20 pmol uL Er s gt 4 0 pmol uL 296 Cy5 Cy5 5 0 12 2 4 pmol uL 647 gt 2 4 pmol pL 296 Alexa Fluor 488 0 40 8 0 pmol uL Alexa 0 40 pmol uL Fluor 594 gt 8 0 pmol uL 296 Alexa Fluor 546 0 30 pmol uL gt 6 0 pmol uL 296 Note Dye Pedestal 3 2 3 Nucleic Acid NanoDrop 2000 2000c
144. 2 125 000 3 0 015 gu 24 Standard3 1000 3 0 111 010 d Standard4 1500 3 0 187 008 d 3 0250 Standard 6 0 06 t n ps 004 002 b d 000 es 200 alo elo 800 1000 120 140 190 1800 200 ug ml R squared 0 997 JA Measure bgiml Avg Abs 1 2 3 Reference 0000 0001 0000 0001 0 002 Standard 1 25 000 0 003 0 004 0003 0 003 Standard 2 125 000 0 015 0019 0015 0010 Standard 3 1000 000 0 111 0112 0111 0 111 Standard 4 1500000 0 187 0 185 0 189 0 188 2000 000 0 250 0 250 0 244 0 258 Protein Pierce 660 nm NanoDrop 2000 2000c Blank Dye Blank Reference Note Blank e 2 Standard 1 Reference BCA 1 Standard 2
145. Beam height 8 5 mm 37 0 5 C 150 850 rpm 10 5 2 1 mm 0 4 ng uL dsDNA 10 mm Path 750 ng uL dsDNA 1 mmPath lt 3 2 1 kg NanoDrop CE UL CSA NanoDrop 2000 2000c US patent 6 628 382 6 809 826 7 Pedestal 1 2uL Nucleic Acid Protein A280 0 5 HL CCD
146. sample and press the measure button EE lank Ria Data Standard Curve Valid Curve Samples Pedestal v Add to report Sample ID 1000 Overlay spectra i Concentration pg ml 949 905 Absorbance at 595 nm 0 124 Use cuvette 040 O Standards Um Measure Conc AvgAbs Reference 0 000 5 10 038 eR 255 Standard 1 31250 5 0039 8 Standard2 62500 5 0045 8 025 Standard 3 125000 5 10 053 E Standard 4 250000 5 0 065 t 020 Standard 5 500 000 5 10 084 Standard6 1000 5 0 130 fu Standard7 2000 5 0 172 0 10 lu e 000 0 05 580 600 620 640 660 680 700 720 740 Wavelength nm 663nm 0 092Abs amp Sample ID Protein Conc Unit A595 1 125 111 426 ugiml 0 050 i i 2 125 137 353 ugiml 0 052 Reports 3 125 128 009 ugiml 0 051 4 125 118 038 ugiml 0 050 ow 00000 5 125 132 058 ugiml 0 052 t 6 1000 1063 535 ugiml 0 132 949 905 7 Lach Dave a 1mm Pedestal Cuvette 2000c Y s Data e Standard Curve R2 Inter
147. 0 as aw as usw os EU o5 nen ass 050 045 040 025 030 025 De 020 ii PL 015 TEL 010 e d 005 eben 0001 250 xo 0 400 450 500 550 500 50 700 Wsrekmgh nen fcq 1 mm Pedestal Cuvette 2000c Y e Absorbance correction 660 nm Abs 600 nm e User cursor nm e User cursor Abs
148. 650 nm 0 118 Use cuvette 040 Standards ps Measure Conc Avg Abs OE Reference 0000 5 0003 Heat to 37 C ia Standard 1 0 125 5 0 017 8 Standard2 0250 5 0036 om Standard 3 0500 5 0087 E Standard 4 0750 5 0094 oom Standard5 1000 5 0 117 T Standard6 1500 5 0149 015 Standard7 2000 5 0173 010 a 0 05 m antt 00 005 400 420 440 460 480 500 520 540 560 580 60 620 640 660 680 700 720 740 Wavelength nm 487nm 0 041Abs Sample ID Protein Conc Unit A650 1 125 0 142 mg ml 0 020 2 125 0 142 mg ml 0 020 3 125 0 133 mg ml 0 018 E 125 0 132 mg ml 0 018 5 125 0 135 mg ml 0019 6 10 1 030 mg ml 0 120 7 1 mm Pedestal Cuvette 2000c Y s Data e Standard Curve R2 Interpolation Linear Polynomials Linear e Valid Invalid Currve
149. Ctrl b Measure F1 F5 Ctrl m Re Blank F2 F2 Ctrl r Print Screen F4 F4 Ctrl p Print Report F5 Ctrl r Shift F4 Show Report F7 F7 New Workbook Ctrl n Ctrl n Reset Calibration check F11 Home Ctrl h Close Workbook Ctrll e Ctrl e Close All Workbooks Ctrl q Configure Report Ctrl f Ctrl f Layout Report Ctrl Ctrl Kinetic Re Blank Stop e Accounts Account Fle Help Accounts set what capabilities are available to each user or group of users Select an item on the left and then add the users allowed to access that item Applications Report Master Page Preferences Accounts Allow access to Options Applications Options Report Master Page Organization v Options Preferences Options Accounts Reports Configure Reports Reprocess Editor Delete method Use current settings as default Overwrite files Allow access to Groups Classic List names from v Remove lt lt Add Allow access to Users and g
150. Documents and Settings All UsersDocumentsVThermo NanoDrop2000 2009_02_25_Protein Bradford twbk E Load your sample and press the measure button Meme Rnt Denk Re Blank Data Standard Curve Valid Curve Samples Pedestal 7 Add to report Sample ID 125 Overlay spectra 0 170 Concentration pg ml 132 058 0 160 Absorbance at 595 nm 0 052 Use cuvette 0 150 e Standards 0 140 up Measure Conc AvgAbs i ita de Reference 0 000 5 0 038 SEAE i Standard 1 31250 5 0 039 0120 r d Standard 2 62500 5 0045 TE P Standard 3 125000 5 10 053 ad Standard 4 250000 5 0 065 i 2010 d Standard 5 500000 5 0 084 om 2 Standard6 1000 5 0 130 Standard 7 2000 5 0172 080 8 p ea 0 070 y 3 0 060 d 0 050 X Pi 0040 g 0 200 400 600 800 1000 1200 1400 1600 1800 2000 ugiml R squared 0 998 A Sample ID Protein Conc Unit A595 1 125 111 426 ugiml 0 050 2 125 137 353 ugiml 0 052 3 125 128 009 ugiml 0 051 4 125 118 038 ugiml 0 050 5 Lach Dave e 2 Standard 1 Reference BCA 1 Standard 2 e 7 Standard Replicate
151. Excel Report Tab Separated Values tsv Excel 2 7 2 Spectrum Excel XML Spreadsheet xml Excel Spectrum Tab Separated Values tsv Excel
152. Linear Polynomials Linear e Valid Invalid Currve Standard Invalid Curve Valid Curve amp amp Note Standard e Samples Valid Curve Sample ID Sample ID e Standard Standard e Concentration mg mL mg mL TF e Absorbance at 660 nm 650 nm 1mm 3 38 3 Protein Pierce 660 nm Protein Pierce 660 nm
153. andard 1 Standard Standard Additional Measurement Measurement Report Build formula
154. c 1 1 1 1 Pedesta 1 2 Pedestal kk 1 2 Guvette E E COME 2s inte amittat Betti uta t tt metet is me d trei seda et 1 3 Cuvette xE FO SEA BO73 f FR RR Lue trennen 2 1 3 Blank EE 1 4 6 DN eoi UBI OS umet dent tute 1 5 AMO D ip hon EEE ETE 2 1 m DA ain Rs a O DAE S E 2 1 SY EE SE 2 1 2 3 KS 2 3 nb PIE 2 3 rav C E Lac RT MEE P MER 2 6 2 7 DAI aAA EA 2 7 SAET a zu n notte reed ERR PR UR It E ERO ERR IRE I RIED E E CIR 2 7 Autosave JT b nae iEn INUENIRI eI RI eR 2 9 OPOS zig uere expe ote E a iq eio AID om IB Gui 2 9 Ne deii EU NIE cet 3 1 Mise cec Ani NN ME Nor Ren OMA e m dcm tns titu t fer e Ment 3 1 3 1 MRS RR EE EE EE EE 3 2 Nucleic AGId io NR RN hp 3 3 Dor PCR E E 3 3 TRO in PEE E e humi unie gne e i eda dre cU dare 3 3 3 4 Nucleic Acid 3 6 Oldgo CGI 3 6 Micro Array ode besten nieder osenitumeoniedo nte RED Em 3 8 Dor cU MM 3 8 Dye Ghromophore Editot 5 ri rU e ERBRECHT PER EE DER AES 3 8 3 8 Micro Array 772 4r 3 23 CO R E enses gnusedii dps nSu e enc Bh nS 3 9 Suo
155. e Report Configuration Reprocess Note Reprocess Reprocess Autosave Sample ID Sample ID Note Autosave Autosave Autosave twbk Autosave PC OS Vista C IUserslPublic Documents ThermolAutosavel XP C IDocuments and Settings All UserslShared Documents ThermolNanoDrop2000 Autosavel 7 7 7 Autosave 24
156. e Kinetic Tab Method name Description Result label fii Time unit Seconds 2 e Measure stage Stage Interval Stage Duration Enter 2 0 e Wavelength to Monitor
157. e Standard Standard e Concentration mg mL mg mL C e Absorbance at 562 nm 562 nm Cu BCA 3 26 3 BCA BCA Protein BCA C Documents and Settings Dave Ash Wy Documents 2009_02_11_Protein BCA twbk Load your standard and press the measure button Data Standard Curve Valid Curve O Samples Pedestal v Add to report e 7 Overlay spectra 0 2 Use cuvette d P d a 0 20 p Standards Pa Measure Conc AvgAbs d yp B MN 3 0003 Heal ta Te 016 P Standard 0 250 3 10 043 rr d Standard2 0 750 3 10 126 ondes 7 d Standard3 1500 3 0247 i 01 A Standard 4 p 0 10 s 0 08 2 ai 06 P d 004 LR n 1 P d 8 000 o 00 1 102 10 0 05 06 0 08 0 1 Er a 1 1 1 mgiml 565nm 0 007Abs Linear MA mg ml Avg Abs 1 2 3 0 000 0 003 0 005 0 007 0 006 Standard 1 0 250 0 043 0 052 0 040 0 036 Standard 2 0 750 0 126 0 126 0 130 0 122 Standard 3 1 500 0 247 0250 0 241 0251 e 2 Standard 1 Reference BCA
158. ie Help ir El Load your sample and press the measure button Measure Print Blank Re Blank Data Standard Curve Valid Curve Samples Pedestal v Add to report Sample ID 10 Overlay spectra 16 Concentration mg ml 1 010 Absorbance at 650 nm 0 118 Use cuvette e 0 14 O Standards Measure Conc AvgAbs 12 d Reference 0 000 5 0003 Heat to 37 C P Standard 0125 5 10 017 Standard2 0 250 5 10 036 10 d 5 M Standard 3 0 500 5 10 067 a Standard 4 0750 5 0094 P Standard5 1000 5 10 117 GM Standard6 1 500 5 10 149 0 06 zt Standard7 2000 5 10 173 D 04 v 7 d 0 02 AC 000 0 0 02 0 05 0 8 10 1 14 16 18 20 mgiml R squared 0 999 amp L3 Sample ID Protein Conc Unit A650 1 125 0 142 mg ml 0 020 2 125 0 142 mg ml 0 020 3 125 0 133 mg ml 0 018 4 125 0 132 mg ml 0 018 5 125 0 135 mg ml 0 019 6 1 0 1 030 mg ml 0 120 e 2 Standard 1 Reference BCA 1 Standard 2 e 7 Standard Replicate Standard e Standard
159. ization IA e Allow access to Users and groups Select or enter a name lt lt Add Allow access to e Users and groups 2 11 3 3 NanoDrop 2000 2000c
160. lShared Documents ThermolNanoDrop2000lCustom Methods Vista C lUserslPubliclDocuments ThermolNanoDrop2000 Custom MethodslC assic 4 5 4 Method Editor 1 4 6 Method Editor Measure Report Add to Report Add to Report Overlay Spectra Standard Reference 7 Standard Reference Standard Note 2 Standard 1 Reference 1 Standard 2
161. m path 280 nm 10 mm 260 280 260 nm 280 nm Baseline correction 340 nm Note Baseline correction Protein A280 1 Protein A280 Wavelength verification OK Type 1Abs 1 mg mL Conc
162. o Interpolation 2nd 3rd Interpolation Standard Standard Standard 4 2 4 Method Editor Standard 2 Standard 1 Reference BCA 1 Standard 2 7 Standard Replicate Standard Standard Standard Replicate Standard St
163. polation Linear Polynomials Linear e Valid Invalid Currve Standard Invalid Curve Valid Curve Note Standard e Samples Valid Curve Sample ID Sample ID e Standard Standard e Concentration pg mL ug mL TF e Absorbance at 595 nm 650 nm 3 34 3 Bradford Bradford E Protein Bradford C
164. roups Users Select or enter a name 2 e Allow access to Options Applications Report Master Page Preferences Accounts 2 10 2 Reports Configuration Report Reprocess Editor Kinetic Use Current Settings as default Overwrite fies e Allow access to Groups Groups
165. tion e Print Idus d Note lt lt Report 3 e Preview Preview Options Print Options e Print e Export xml tsv twbk Report Excel XML Spreadsheet xml
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