IO テスト PE 標識抗体 CD16 ION16
Contents
1. IO CD20 Nalm 6 1 Raii 2 959 CD20 JURKAT 3 0 5 1 NIm 6 pre B ALL 2 Raji Burkitt lymphoma 3 JURKAT T cell Leukemia PBS 4 CD3 HPB ALL CCRF CEM 3 10 3 5 52. 6 PBS 2mL 7 400 x 9 3 8 7 8 9 0 5 1mL 0 59 2 PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 xg 30 4 Ficoll Paque 5 PBS
3. 10 11 1 20 ux L 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 I IM3515 12 5L 0 19 PBS VersaLyse
4. 4 5 6 7 Ficoll Paque 8 9
5. IOTest T B NK
6. A07766 2009 11 AX 2006 9 202EZY00111000 amp Oy W Y I NK IO PE CD16 ION16 1 2 3 4 IO PE CD16 ION16 PE IgG 20 L CD16 50 65kD 48kD CD16 HAIE IgG Fo LE TA Foca RI ec NK 50 65kD DIR 48kD
7. PBS 1mL IOTest 3 A07800 18 IM3515 67 5uL 0 59 PBS 3 A09777 IM3648 VersaLyse IM1400 OptiLyse B A11895 IA A11894 OptiLyse C A07799 I IM3514 IOTest 3 4 IOTest Mouse IgG1 PE A09142 A09141 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x 103 mm3 10x103 mm PBS 3x 103
8. 2 2 EATS Lb SS RM SmI EPICS XL vs PE 36 Lymphocytes Leukocytes 68 88 Count 48 188 1888 1 1 18 188 1888 PE CYTO TROL 6604248 Fc IOTest CD45 FITC CD14 PE A07738 I IM1201
9. 1 0 196 3 4 5 6 8
10. 5 0 5 1mL 0 5 PBS 24 A O 6 7 8 9 0 as 2 4 FS 90 SS PE Log
11. 2 8 C 400 xg 8 KEIL PBSZMATKHBHLET 2 8 CC 400 x g 4 AIK PBS 2 8 C 400 x g 3 PBS 5x103 El mm3 5 x 108 mL 90 HER 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5x10 mL 100 L 3 20L IOTest Mouse lgG1 PE 20L 2 8 30 PBS 2mL 2 8C 400 xg 5
12. GP CD16b 2 CD16 NK ADCC 3G8 GPI CD16 3G8 IgG1 PE Phycoerythrin 486 575nm 560 590nm 1 CD16 CD16 PE 1 IO 2 EDTA 3 6
13. gt IO PE CD16 ION16 2 80 12 IO PE CD16 ION16 A07766 100 2mL 1 Whiteside T L Herberman R B The role of natural killer cells in immune surveillance of cancer 1995 Cur Opin Immunol 7 704 710 2 Hannet 1 Erkeller Yuksel F Lydyard P Deneys V DeBruyere M Developmental and maturational changes in human blood lymphocyte subpopulations 1992 Immunol Today 6 13 215 218 3 Robertson M J Ritz J Biological and clinical relevance of human natural killer cells 1990 Blood 12 76 2421 2438 4 Ritz J Trinchieri G Lanier L L NK cell antigens section report 1995 Leucocyte Typing V White Cell Differentiation Antigens 3 4 7135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC oe COULTER Na IMMUNOTECH A BECKMAN COULTER COMPANY 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France A07766_1109 4 4
14. mm3 1 25 C 500 x g 5 2 3 4 LH700 5 PBS 5 10 x 10 mm3 1 12mmd x 75mm gt gt 2 100L 3 20L IOTest Mouse IgG1 PE RE 20L 4 15 5
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