虚血再灌流障害に伴う 骨格筋筋萎縮の予防に関する研究
Contents
1. Si 6 2 8 Wistar REZ y b 21 3 15 7 15 7 Vo 15 72. We 2 4 90 2
3. 7 ATP ATP Na K ATPase mg Er AL i Ie q
4. 4 BE 525 OD 315 6 1 90 91 7 351 2 mmHg 7 Kam
5. Bd 15 1 15 1 48 Bar100 um T h S 3 ZZ mau 15 2 48 18 3 72
6. mi 54 55 Ber 1
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9. E Nakashima 9
10. 12 DC1 6 AG101 D E Hokan L7 B son fEfRL USA xj 40 mg kg b w 3 No 19 Hokansori EX RAPID CUFF INFLATOR 2 2 300 mmHg 90 2 Mz PIHEWUOU 7 WGEBROESE Wistar 8 9
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12. 3 4 S 3i i ai x mh E Em bi MIT OBuukCUG dEd 5 ZOE
13. DIZ I I
14. Appel11 60 60 BLEED 15 30 69 12 99 EK 40 86
15. g mg 2 26 45 EE ProPlus6 2 USA L 10 um uil
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17. E Kondo 9
18. 8 Wistar 60 15 E F B Hi 49 6 Er 00 36 7 B E 23 3v DERMAL TOD ZEN UT TORR 04 11 T REC IE UT RSSENRO SEE C
19. Lo A 11 3 10 um 8 Bar100 um 1 SPSS15 0J for Windows SPSS Inc USA Dunnett 1 24 48 72 loo 120 5 12 FREDE OO Er R3 EAE ORERE
20. 5 72 GSH 5 0 6
21. m 4 2 8 Wistar REG 25 5 5 3 3 Hi 5 3 3 5 UE BE 4 4 4 4 4 5 3 4 25 4 3 4
22. Ed 24 0 54 mg g 0 60 xx xk 0 50 0 40 0 30 0 20 mg gb w 0 10 0 00 n 4 n 5 n 5 VS p lt 0 01 28 37 5 37 73 pm IF 44 49 um 44 18 um 25 43 24 um 50 00 45 00 40 00 35 00 30 00 25 00 20 00 15 00 10 00 5 00 0 00
23. E gt AX 5 2 cL AX AN N A 0 E 72 fn 72 1 300 mmHg mg kg b w WR 90 8 Wistar 15
24. 20 3 120 18 18 120 Bar100 um 21 3 4 E
25. 3 3 3 3 1 Jin FEE O HENE OO POT g mg 3 3 mg g um 0 54 0 06 43 24 2 89 1 0 54 0 03 44 08 2 73 24 0 56 0 04 44 88 2 25 48 0 52 0 06 44 69 2 50 72 0 46 0 08 39 17 2 55 96 0 432 0 03 41 92 1 08 120 0 46 0 05 42 53 2 81 a p lt 0 01 b p lt 0 05 1
26. E rei d rn EE UCe 7 7v ERO TES S Jit 2 Bd 1 ow SET PRESSURE gt mmHg 2 wi E20 RAPID CUFF INF R Hokansa 1
27. e eo in e D eo e N eo mg g b w eo o o 50 00 45 00 40 00 35 00 30 00 H 25 00 20 00 15 00 H 10 00 5 00 U 0 00 1 24 48 72 96 120 19
28. 9 2 100 150 mmHg 250 mmHg 351 mmHg 77 1 A 1 N 250 mmHg 251 300 mmHg 301 350 mmHg 351 mmHg Kalla TP 2 8 24 64 8 7 Younger AS 11 3 38 49 1 5 Te jwani NC 19 996 4T 496 32 496 Sapega 90 120
29. VUIBUSEEO SERITZU C U8 OREEE e TEE C 24 EE 4A N 4 4 1 BUS 9
30. 11 Knight KR Messina A Hurley JV et al Muscle cells become necrotic rather than apoptotic during reperfusion of ischaemic skeletal muscle International Journal of Experimental Pathology 1999 80 169 175 12 Walters TJ Kragh JF David GB Influence of fiber type composition on recovery from tourniquet induced skeletal muscle ischemia reperfusion injury Applied Physiology Nutrition and Metabolism 2008 33 272 281 13 Ong SC Ischemia induced atrophy in skeletal muscle fibers abtract International Journal of Sports Medicine 1989 10 373 14 1992 pp102 15 2003 54 6 711 713 LE 56 16 17 18 19 20 21 22 23 24 2005 56 9 16 19 CS BOSS
31. ERO RZ KIE K AE 3 2 24 BREED 72 120
32. dit x mh WER CIA B 2 0 5 3 24
33. odi MA ZN 96 ir LA 96 24 96 96 48 72
34. n DORE L 16 1 l T nd 16 2 72 19 3 96 17 17 96 Bar100 um
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36. 4 REM DR SKIF EOD 3 11 24 26 27 52 FEJ 48 E EAR E 21 15 79 BE F Bk Hi
37. Gk 28 44 E r E mg gb w eo n 7 n 7 n 7 n 7 D 0 01 p lt 0 05 32 2 10 11 10 X LLI ERV p lt 0 01
38. 9 9 mg g um 0 58 0 02 51 85 5 82 0 27 0 04 4 36 003 1 68 0 32 0 07 30 67 2 07 0 55 0 09 45 18 3 53 b TI a p lt 0 01 E 38 p lt 0 05 c p lt 0 01 d p lt 0 05 0 58 mg g 0 27 mg g 0 32 mg g 0 55 mg g
39. 6 6 3 4 3 4 H T gt gt gt gt 3 24 4 i25 D MI HE CU 26 4 27 24 3 Bar100 um 30 ER 4
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46. 1998 25 6 381 387 TE 2010 n RH 58 42 43 44 45 46 47 Kasper CE White TP Maxwell LC Running during recovery from hindlimb suspension induces transient muscle injury Journal of Applied Physiology 1990 68 533 539 Kondo H Kodama J Kishibe T et al Oxidative stress during recovery from muscle atrophy European Biochemical Societies 1993 326 1 2 3 189 191 1992 pp39 Nakashima K Ishida A Yamazaki M et al Leucine suppresses myofibrillar proteolysis by down regulating ubiquitin proteasome pathway in chick skeletal muscles Biochemical and Biophysical Research Communications 2005 336 2 660 666 C E 2008 16 5 540 544 E Mod
47. 8 Wistar 16 9 mmHg TE 079 TAG CBC EX RT EF v ROME 124 6 150 mmHg 300 mmHg 90 9 57 X S3 i Wi star ZA METH E 23 MF Ie
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49. yu 26 3 Bar100 um 31 4 27 4 Bar100 um 1 3 4 C 2 3 4 3 3 3 1 z nik 4
50. 47 6 13 2 F P 252 0842 1 252 0842 18 8814 p lt 0 01 1612 3410 1 1612 3410 120 7661 p lt 0 01 X 3 1439 1 3 1439 0 2355 0 6319 320 4226 24 13 3509 2187 9917 27 SEIL 6 4 H Bie LTOS 5 XE PR E E
51. 24 48 72 96 120 Too Ed 9 1 24 48 72 96 72 Ed 10 13 mg gb w um 3 0 70 0 60 0 50 xx 0 40 0 30 0 20 0 00 n 6 1 n 6 24 n 7 48 n 6 72 n 6 96 n 5 120 n 5 vs p lt 0 01 9 50 00 45 00 40 00 35 00 30 00 25 00 20 00 15 00 10 00 5 00 0 00 n 6 1 n 6 24 n 7 48
52. C x v E 1992 66 2 79 89 2 3 2006 40 4 165 174 4 1991 157 5 253 258 5 1996 179 10 829 834 6 2006 40 4 175 182 7 Siesjo BK 2000 18 13 1798 1804 8 2005 5 2 32 37 9 2002 200 12 950 951 10 MNMS 1991 157 5 326 329 1g Surgery Frontier 2007 14 1 67 73
53. CPK GSH 195 24 umol L 259 08 umol L 287 81 pmol L GSH GSH 8 27 400 350 300 250 pmol L 200 150 GSH 100 50 0 n 4 n 5 n 5 VS p lt 0 05 E21 GSH 39 5 4 1 1 Ca
54. L E 11 2 F P 0 0007 1 0 0007 0 1684 0 6852 0 5095 1 0 5095 122 4340 p lt 0 01 X 0 0077 1 0 0077 1 8572 0 1856 0 0999 24 0 0042 0 6178 27 51 85 um 36 00 um 30 67 um 45 18 um 9 29 1009 30 6 40 8 12 9 um 12 30 Xk 70 00 z xk 60 00 50 00 40 00 30 00 20 00 10 00 0 00 n 7
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64. 4 4 Walters 250 mmHg Appel11 60 60 32 9 3 PRE 7 D
65. um n 4 n 5 n 5 VS p lt 0 05 29 CPK 889 40 IU L 587 60 IU L 383 20 IU L CPK 8 26 8 CPK IU L GSH umol L 889 40 365 46 195 243 51 08 587 60 368 94 259 08 71 22 383 20 81 04 287 81 84 66 a p0 05 38 5 Hed PEWEUIC GS OO 3 EB IOS D DUE EL Be 5 0 SEE 1400 00 1200 00 1000 00 ad 23 800 00 600 00 D o 400 00 200 00 0 00 n 4 n 5 n 5 30
66. ZoNa S Appell 60 90 60 90 79 59 162 6 Knight 2 24 We E C OO BERE RS OO EK 1 24 3 24 8 24 I BREED
67. 4 10 um DED Image ProPlus6 2J USA 200 ZKEA EO 55 1 1 100 100 e CAROR
68. H IEEE ARD RU HE LC 03 3 4 HELEP HEdLBRE DEOE C VAT EE 24
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70. 3 300 mmHg 90 MORE 30 4 6 S 300 mmHg 90 a b c d a
71. GSH GSH CPK Lewis 3 0 mg g 9 0 mg g Yamamura 5 5 CPK 19 Irie 4 24 9 Yamamura GSH CPK 9 C
72. Scheffe 5 4 3 4 4 mg g um 0 53 0 02 42 54 0 80 3 0 46 0 06 34 39 3 90 3 0 48 0 04 39 88 0 10 4 0 46 0 06 38 63 1 80 4 0 443 0 05 38 403 2 42 a p lt 0 05 27 4
73. 6 1 pE S 2 1 7 72 D 8 Wistar 41 7 1 6 72 E 5 JU7 BK 90 40 mg kg b w 40 mg kg b w
74. E 5 E 36 SPSS15 0 for Windows SPSS Inc USA Scheffe 5 5 3 Wi 7 7 mg g um 0 432 0 01 37 73 1 70 0 51 0 03 44 49 2 94 0 52 0 03 44 18 3 11 a p lt 0 01 b p lt 0 05 0 43 mg g 0 51 mg g 0 52 mg g
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76. 72 2 2 4 200m 5 4
77. 4 21 0 60 mg gb w 0 50 0 40 0 30 0 20 0 10 0 00 n 5 3 n 5 3 n 5 4 n 4 4 n 5 2 21 3 4 22 hm 50 00 45 00 40 00 35 00 30 00 25 00 20 00 15 00 10 00 5 00 0 00 n 5 3 n 5 3 n 5 4 n 4 4 n 5 vs p lt 0 05 22 28 3 3 3 10 um 30 hm OG 3
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82. 3 4 DES 4 4 i 4 5 22 m 5 um RRAN um 0 10 10 20 20 30 30 40 40 50 50 60 60 70 70 80 80 90 90 100 0 1 20 68 237 300 137 33 4 0 3 0 77 104 94 215 218 72 19 1 0 3 0 4 20 110 266 271 101 26 2 0 4 0 6 29 110 291 255 90 17 2 0 4 0 3 28 118 291 255 82 21 2 0 350 300 r 3 250 3 X 4 EN 200 4 E 150 100 50 0 10 10 20 20 30 30 40 40 50 50 60 60 70 70 80 80 90 90 100 um 23 29 4
83. n 7 n 7 n 7 D 0 01 p lt 0 05 33 46 6 12 um 51 85 5 82 0 0 36 00 1 68 30 6 30 67 2 07 40 8 45 18 3 53 12 9 a DD 9 p lt 0 01 b p lt 0 05 c EKM oA Ep p lt 0 01 d p lt 0 05 50 45 40 35 30 25 20 15 10 n 7 n 7 n 7 34 2 10 13
84. n 6 72 n 6 96 n 5 1206 f BE n 5 vs p lt 0 05 10 14 3 1 24 48 72 72 REA E 96 120 72 11 12 450 400 350 300 gu 250 HA 200 H is 100 50 0 10 10 20 20 30 30 40 40 50 50 60 60 70 70 6 1 24 48 72 80 80 90 90 100 um 11 EER T 24 48 72 400 350 300 B 250 ES 200 iu 150 100 50 0 10 10 20 20 3
85. 6 30 6 12 99 40 86 But cx E DRAEZ 6 BE
86. 10 hm H amp E GSH ImageProPlus6 2 USA 200 h CPK CPK 15 4C 3500 15 GSH 5 4C 3500 Eliz 20
87. b c d e Fd ATP 289 11 2932 1 1
88. be Y s i f ATOT 90 E CEC 4 gt Sa 3 Kasper 9 B fT
89. 3 72 5 15 DC1 6 Jnd AG101 D E Hokanson ERE e ees d o0 1 30 4 5 USA 35 HJE yes Eu o B 40 mg kg b w TL Ha 7 xu MB L 40
90. 2 HE Excel 2007 2 2 Kruskal Wallis Scheffe 5 43 HE OENAR T D AEO EE 3 o EP 6 3 i
91. 24 48 D R 24 72 BASH HO EE EL BRE 96 t 7 2 3885 Bel 96 72 gt o TZ
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93. No 1 59 7 1 1 1 5 2 3 4 1 8 1 3 2 1 2 1 1 69 1 32 No 2 30 23 4 1 5 1 3 2 3 1 4 1 6 1 9 1 9 1 9 1 8 35 8 20 No 3 52 6 4 3 2 1 6 2 2 5 1 7 1 9 1 9 1 8 1 8 63 3 41 No 4 19 6 4 5 0 7 0 9 0 9 0 8 0 8 0 9 1 0 8 0 8 57 26 3 No 5 19 7 2 5 0 4 0 6 0 7 0 7 0 8 0 9 1 2 1 1 1 1 48 9 24 30 4 6 300 mmHg 90 6 7
94. 0 30 40 40 50 50 60 60 70 70 80 80 90 90 100 4 72 96 120 a gala Li nm 12 72 96 120 15 3 3 3 2 40 45 um 8 1 13 1 13 1 1 Bar100 u
95. E 30 mg kg b w 12 60 6 Kondo gt 42 jg 40 mg kg b w JO t7 X MEHERIE P ctr Ed UIT Uii Ha UT d HR EOIETYUECB ELE g mg 2
96. ERREDUO TRE 2003 5 3 503 507 2000 36 4 131 133 El Kam PC Kavanaugh R Yoong FF The arterial tourniquet pathophysiological consequences and anaesthetic implications Anaesthesia 2001 56 534 545 Kalla TP Younger AS McEwen JA et al Survey of tourniquet use in podiatric suergery The Journal Of Foot amp Ankle Surgery 2003 142 2 68 76 Younger AS Kalla TP McEwen JA et al Survey of tourniquet use in orthopaedic foot and ankle surgery Foot amp Ankle International 2005 26 3 208 217 Tejwani NC Immerman I Achan P et al Tourniquet cuff pressure the gulf between science and practice The Journal of TRAUMA Injury Infection and Critical Care 2006 61 1415 1418 Sapega AA Heppenstall RB Chance B et al Optimizing tourniquet application and release times in extremity surgery The Journal of Bone and Joint Surgery 1985 67 A 2 303 314 Appell HJ Gloser S Duarte JAR et al Skeletal muscle damage during tourniquet induced ischaemia The initial step towards atrophy after orthopaedic surgery European Journal of Applied Physiology 1993 67 342 347 25 Coderre TJ Xanthos DN Francis L et al Chronic post isch
97. Ki TIL FRE Do Pe CE 2 24 3 2012 S B B 3 E 3i Tir Tir Tir Tir 2 1 2 2 2 3 3 1 3 2 3 3 UNS 3 3 1 dr 3 3 2 3 4 Ed Rd QT S E 1f FRE C fe 0O f EM COR T o ERE EO KE 4 1 4 2 4 3 4 4 10 11 13 16 22 25 25 27 32 35 35 37 40 6 MEMUL EFIE Utt Ge OO 5 EB COS 2 ZEE 6 1 6 2 3 i 8l T gt E 42 42 44 48 51 54 56
98. PK CPK CPK Wistar 5 41
99. emia pain CPIP 26 a novel animal model of complex regional pain syndrome Type I CRPS I reflex sympathetic dystrophy produced by prolonged hindpaw ischemia and reperfusion in the rat Pain 2004 112 94 105 2009 36 5 275 280 57 27 Appell HJ Gloser S Soares JMC et al Structural alternations of skeletal muscle induced by ischemia and reperfusion Basic and Applied Myology 1999 9 5 263 268 28 Chan RK Austen WG Jr Ibrahim S et al Reperfusion injury to skeletal muscle 29 30 31 32 33 34 35 36 37 38 39 40 41 affects primarily type II muscle fibers The Journal of surgical research 2004 122 1 54 60 1999 22 2 93 100 2000 193 7 598 603 ERE Surgery Frontier 2004 11 3 7 12 The rapeutic R
100. ern Physician 2007 27 9 1262 1264 48 Yamamura M Miyamoto Y Mitsuo M et al Suppression of Rat Lower Extremity 49 50 51 52 53 Postoperative Reperfuusion Injury with Edaravone Journal of Angiology 2006 15 34 36 Irie H Kato T Ikebe K et al Antioxidant Effect of MCI 186 a New Free Radical Scavenger on Ischemia Reperfusion Injury in a Rat Hindlimb Amputation Model Journal of Surgical Research 2004 120 312 319 Q amp A 6 2011 17 6 85 87 2002 30 2 119 125 fii S07 A EXEC X 7 v h PERO ZERS c E 2000 27 5 174 179 2002 30 2 113 117 59
101. esearch 2004 25 8 1677 1682 2009 24 5 751 753 4 2003 pp2 4 2011 26 2 191 195 WAF 2003 30 56 TETTE 2004 21 1 251 255 2007 95 5 701 706 pp141 147 PT 2003 37 6 503 506
102. m 1 13 2 1 16 3 24 1 XI 14 1 14 1 24 Bar100 pm 1 14 2 24 17 3 48
103. p 45 55 um 1 2 4 EAE X H EE E Z b5 25 1 Blk ri f EP 565
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