RCESD009 small pack 取扱説明書 ダウンロード (日本語PDF)
Contents
1. Q2 A2 Q3 MEA probe A3 day 3 509 MEA probe Q4 MEA probe A4 day 14 day 9 14 Q5 A52. 1 day 0 1 day 1 3 day 3 day 4 14 Transfer Non attachment Non attachment MEA dish MEA dish 2 MEA dish Q1 U A1 day 2 3 day 4 MEA 02 A2 Q3 MEA A3 day 3 5096 MEA dish Q4
3. MEA dish 37 596 CO gt Step D MEA dish day 4 9 day 4 200 LuL 7 6 well dish 100 uL well MEA dish 8 2 MEA dish WWW eDrOcell com 1 P 1000 1 000 LL MEA dish 1 000 LL 9 10
4. well 3 day 7 day 14 Step C day 7 14 ReproCardio Coat for Patch ReproCELL Clamp Solution A 12 mL IRCESDO020 ReproCardio Coat for Patch ReproCELL Clamp Solution B 0 4 mL IRCESD020 ReproCardio Coat for Patch ReproCELL Clamp Solution C 1 4 mL IRCESD020 24 well plate flat bottom MATSUNAMI CO130011 Step A day 0 1 CoversliDp 15 Coat B Coat C 1 Coverslip 2 Coverslip1 24 well plate 1well 3 ReproCardio Coat for Patch Clamp B Coat B Coat C 30 5 1 4 Coat B Coat C Coat A 5 Coat B Coat C
5. 8 2 MED dish 1 P 1000 1 000 uL 1 000 LL 9 WWW eDrOCcell com 10 11 4 well 200 LL Step E day 9 14 x10 37C 37
6. MEA dish day 0 day 3 day 9 day 14 Step B U bottom 96 well Plate day 0 Thawing 3 Cell clump Step B formation U bottom 1 300 uL U bottom 96 well plate 3 1 5 x 10 cells 300 uL Transfer Thin layer Step D formation MEA dish 2 379C 59 6 CO gt 100 uL 200 uL 1 dayi9 14 3 day 2 100 pL 100 uL 4 day 1 3 day 1 30 50 day 2 80 day 3 100 7 WWW eDrOcell com For Research Use Only amp po 2 ReproCELL Step C MEA dish day 3 4 dish day 3 well day 3
7. 5 CO2 Air 4 day 3 MEA dish 5 day5 1 1 000 uL 6 37 59 CO gt CO 7 30 umx30 um 1 page 2 4 Step A day 0 1 RCESD009 1 1 0 x 10 cells mL 1 0 mL 0 5x10 cell mL 2 37 C
8. A 7 5 2 90 30 2 9 A 1 mL A 2 10 300 xg 350 xg 5 2 11 B 2 12 A 2 13 2 14 B 10 mL A 2 15 300 xg
9. 13 AQUACOSMOS 2 day 4 1 HAMAMATSU PHOTONICS 100 uL well 100 uL well 5 5 3 37C 596 CO gt Step E day 9 14 1 96 well 20 mL ReproCardio Assay Medium Cal 520 AM DMSO 5 uM Cremophor EL DMSO 0 05 2 DMSO 20 2 100 LL 3 Step E 1
10. 9 A7 U 96 well plate 3 For Research Use Only po 2 ReproCELL ReproCardio2 MED64 SYSTEM ALPHA MED SCIENTIFIC aMED Catalog number ReproCardio Assay ReproCELL 1 1 15 000 Medium RCESD007 NR 2 well day 3 MED dish MM ie cc CE a 33016 015 3 day 3 5 CO2 Air 4 day 3 MED dish 5 day 5
11. MEA dish 11 6well dish 150 uL wel Step E day 9 14 1 4 x10 37 MEA dish 35 mm dish 12 37 C MilliQ 30 mL 5 CO gt Air P 1000 ReproCardio Assay Medium 800 LL 10 13 6well dish ReproCardio Assay Medium 150 uL well
12. MEA dish 10 14 day 9 day 14 day 9 day 14 8 For Research Use Only po 2 ReproCELL 7 x10 8 1 9 4 10 2 15 7 9 16 4 10
13. A4 lt 200 day 3 day 7 Q5 A5 Q 6 A 6 Coat B Coat C 15 17 For Research Use only po 2 ReproCELL ReproCELL Inc Website https Wwww reprocell com E mai
14. 2 1 2 9 3 1 1 0x10 cells x1 x1 ReproCardio Culture Medium 2 FBS 80 mL x 1 ReproCoat 30 mL x1 CE 96 well plate x1 96 well plate x1 20 C 1 ReproCarido Culture Medium 2 FBS 2 WWW eDrOcell com Fetal Bovine Serum FBS Life Technologies Gibco Cat 10437 028 e Penicillin Streptomycin Life Technologies Gibco Cat 15140 1 1 1 ReproCardio Culture Medium 2 FBS
15. MED dish Q6 A6 day 10 Q7 5 CO Air A7 596 CO Air WWW eDrOcell com For Research Use Only po 2 ReproCELL ReproCardio2 MEA system multi channel systems Catalog number ReproCardio Assay ReproCELL 1 1 15 000 Medium RCESD007 NR 2 well day 3 MEA dish MM ie cc GS a 33016 015 3 day 3
16. 100 LL 4 day 1 3 day 1 30 50 day 2 80 day 3 100 Step C MEA probe day 3 4 well day 3 MEA probe day 3 10 For Research Use Only amp po 2 ReproCELL 5 909 509 MEA MEA probe robe 5 mg Fibronectin Human Plasma 50 ug mL PBS MEA probe1 20 HL 50 ug mL Fibronectin probe 4 PBS 2 mL 37 C 59 6 CO gt 1 PBS 1 mL
17. day 14 x10 5 For Research Use Only po 2 ReproCELL 9 4 10 2 16 7 9 17 1 day 0 1 day 1 3 day 3 day 4 14 Transfer Non attachment Non attachment MED dish MED dish 2 MED dish Q1 U A1
18. well 500 uL ReproCardio Culture Medium 2 FBS 3 500 pL 2 5x10 cells well 4 379C 596 CO gt WWW reprocell com 16 For Research Use only amp po 2 ReproCELL 5 Step C day 2 7 1 ReproCardio Culture Medium 2 FBS day 3 day 5 day 7 1 2 ReproCardio Culture Medium 2 FBS 37 15 3 P 1000 500 LL 500 uL 4 37 59 CO gt 6 7 well Step D day 14
19. Q 3 well A3 FDSSuCELL HTS Q4 FDSSuCELL A4 Cal 520 2 uM Movies of beating thin layers available https www reprocell com en cardio laver WWW eDrOcell com For Research Use Only po 2 ReproCELL ReproCardio2 1 ReproCardio Coat for Patch Clamp Solution B C 15 2
20. 2 ReproCoat 50 uL well 12 3 ReproCoat 4 P 200 200 HL 5 96 well plate V day 3 3 4 well 6 day 9 day 9 day 14 13 For Research Use Only po 2 ReproCELL Step D day 3 7 1 ReproCardio Culture Medium 2 180 180 FBS 37 15
21. day 7 14 day 7 x200 ED WWW eDrOcell com Q1 Web whole cel outside out A1 Whole cell Q2 A 2 iPS 140mM KCI 1mM MgCI2 5mM EGTA 10mM HEPES 5mM MgATP KOH pH7 2 150mM NaCI 4mM KCI 1mM MgCI2 1 2mM CaCI2 10mM HEPES 10mM Glucose NaOH pH7 4 Q 3 A3 Q4
22. Vortex 10 100 uL well 4 37C 596 CO gt 120 5 x10 37 6 180 LL 7 ReproCardio Assay Medium 80 uL well 8 10 9 Ex 490 nm Em 514 30 6 ECLIPSE Ti Nikon FDSS uCELL HAMAMATSU PHOTONICS 10 10 ulL well ReproCardio Assay Medium 10x 10 LL well 11 10 30 12 10 11 14
23. lt 8 lt Step E day 9 14 1 x10 WWW eDrOcell com 9 37 9 379C 5 P 1000 ReproCardio Assay Medium 1 000 LL 10 MEA probe 10 10 11 day 9 14
24. 15 mL 2 Coat B Coat C 20 6 Coat A 15mL Coat B Coat ReproCardio Coat LL C well 1 so 7 24 well plate well 500 LL 6 8 6 9 6 ReproCardio Culture Medium 2 37 5 CO 3 page 2 3 day 1 1 1 0 x 10 Step B 1 RCESD009 cells mL 1 0 mL 0 5 x10 cell mL 4 37 C 2 Step A
25. MEA A4 day 14 day 9 14 Q5 A5 MEA dish Q6 A6 day 10 Q7 5 CO Air A7 5 CO gt Air WWW eDrOcell com For Research Use Only po 2 ReproCELL ReproCardio2
26. 1 1 000 pL 1 page 2 3 Step A day 0 1 RCESD009 1 1 0 x 10 cells mL 1 0 mL C 0 5x10 cell mL 2 6 37 59 CO gt CO gt 7 20 umx20 um 37 C MED dish day 0 day 3 day 9 day 14 Step B U bottom 96 well Plate day 0 Thawing 3 Cell clump Step B formation U bottom 1 300 uL
27. U bottom 96 well plate 3 Transfer Thin layer RS 1 5 x 10 cells 300 HL IS La 2 37C 596 CO 100 LL 200 pL 3 day 2 100 LL 100 LL lt 4 day 1 3 day 1 30 509 day 2 80 day 3 100 Step C MED dish day 3 4 well day 3 MED 4 WWW eDrOcell com For Research Use Only amp po 2 ReproCELL 9 dish day 3 5 909 509
28. MEA probe Fibronectin P 200 50 pL U bottom plate 50uL MEA probe MEA probe 37 C 5 CO gt Step D MEA probe day 4 9 1 2 day 4 200 uL 6 MEA probe 1 P 1000 1 000 pL 1 000 uL 7
29. 4 1 2 20 mL FBS 1 mL Penicillin Streptomycin 80 mL ReproCardio Culture Medium 2 FBS 1 3 10 mL 4 3 2 3 2 1 2 9 A lt B C 2 1 10 mL 3 15 mL A B C 37C 15 2 2 A 2 3
30. 90 A2 A Q3 90 A3 Q4 1 5 A4 2 1 2 9 Q5 A5 Q6 CAN A6 p 1000 10 07
31. day 9 day 14 x10 4 10 2 12 6 8 11 For Research Use Only po 2 ReproCELL 1 day 0 1 day 1 3 day 3 LL a Transfer Non attachment Non attachment MEA Probe 2 MEA probe day 4 14 MEA Probe Q1 U A1 day 2 3 day 4
32. 2 4 1 2 5 1 4 4 30 5 2 6 2 7 2 For Research Use Only amp po 2 ReproCELL 90 6 90 2 8
33. MED dish 100 mm dish MED dish MED P210A 5 mg Fibronectin Human Plasma Invitrogen 330160 015 50 ug mL PBS MED dish1 20 nL 50 ug mL Fibronectin MED Dish PBS 3 mL 37 C 596 CO gt 1 PBS 1 mL MED dish Fibronectin P 200 150 pL U bottom plate MED dish 6 4well dish 75 UL MED dish 37 C 596 CO gt Step D MED dish day 4 9 1 day 4 200 uL 7 4 well dish 125 LL
34. Maestro Axion 1 1 15 000 2 well day 3 MEA probe 3 day 3 4 day 3 MEA probe 5 day 5 1 1 000 uL 6 37 596 CO gt CO gt MEA probe day 0 day 3 day 9 day 14 Thawing Cell clump Step B formation U bottom Transfer
35. MED dish 35 mm dish 12 37 C 5 MilliQ 10 mL 5 CO gt Air MED dish P 1000 ReproCardio Assay Medium 1 000 LL 10 13 4 well ReproCardio Assay Medium 200 uL well MED dish 10 14 15 day 9 day 14 day 9
36. WWW eDrOcell com 1 Step A day 0 1 RCESD009 1 1 0 x 10 cells mL 1 0 mL 0 5x10 cell mL 2 37 C Step B U bottom 96 well plate day 0 3 1 200 uL U bottom 96 well plate 3 1 0 x 10 cells 200 uL 2 37 596 CO gt 3 day 2 100 LL 100 LL 4 day 1 3 day 3 100 day 1 30 50 day 2 80 Step C day 3 1 96 well plate V
37. WWW reprocell com For Research Use Only po 2 ReproCELL 1 Q 1 day 0 1 day 1 3 day 3 day 4 14 A 1 1 a gt EE L 4 Q2 non attachment non sttachmaent ttachmant Nch A2 8 day 3 96 well plate 2 3 2
38. 350 xg 5 2 16 9 mL 1 mL 2 17 P 1000 1 mL 6 6 1 0x 10 cells 1 mL WWW eDrOCcell com 3 8 C 9 45 qMED page 4 MCS page 7 Axion page 10 page 13 page 16 page 18 Q1 FBS A1 Q2
39. MEA probe Q6 A6 day 10 WWW eDrOcell com For Research Use Only 12 po 2 ReproCELL ReproCardio2 1 well 2 7 3 Cal 520 day 0 day 3 day 9 day 14 Cell clump Step B formation 0 3 U bottom Cell clump Step D attachment ReproCardio Assay Medium ReproCELL 100mL RCESD007 ABD Bioquest Cal 520 MAM 21130 SIdIma Cremophor EL C5135 500G Sumitomo V 96 well plate bakelite MS 9096V WAKO 049 07213
40. amp po 2 ReproCELL ReproCardio 2 Cat RCESD009 Verslon 1 0 ManualS 1 ReproCardio 2 Daqge 2 2 ReproCardio 2 MED64 SYSTEM ALPHA MED SCIENTIFIC page 4 3 ReproCardio 2 MEA system multichannel systems DaQde 7 4 ReproCardio 2 Maestro Axion Dage 10 5 ReproCardio 2 Dage 13 6 ReproCardio 2 page 16 WWW eDrOcell com For Research Use Only po 2 ReproCELL ReproCardio2 4 1 5 45
41. day 2 3 day 4 Q2 A2 Q3 MED A3 day 3 509 MED dish Q4 MED A4 day 14 day 9 day 14 Q5 A5
42. MEA 9096 MEA dish 50 MEA dish p100 mm dish MEA dish 200 30 MCS60MEA200 30iR Ti gr 5 mg Fibronectin Human Plasma Life Technologies 330160 015 50 ug mL PBS MEA dish 1 20 HL 50 ug mL Fibronectin MEA dish PBS 3 mL 37 C 59 6 CO gt 1 PBS 1 mL MEA dish Fibronectin P 200 150 pL U bottom plate MEA dish 6 6 well dish 33 LL
43. Thin layer Step D formation MEA probe WWW eDrOcell com Catalog number ReproCardio Assay ReproCELL 49C Medium RCESD007 Life FiDronectin Human Technologies 49C Plasma 33016 015 1 Step A day 0 1 RCESD009 1 1 0 x 10 cells mL 1 0 mL 0 5x10 cell mL 2 375C Step B U bottom 96 well Plate day 0 3 1 300 uL U bottom 96 well plate 3 1 5 x 10 cells 300 uL 2 379C 596 CO gt 100 uL 200 uL 3 day 2 100 LL
44. l info_jpQreprocell com WWW eDrOcell com For Research Use Only 18
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