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36. 2014 4 E 12 ARAP 3 4 3 7 Summary of Mid term Review I Outline of the Project Country Republic of Panama Project title Comparative studies of the reproductive biology and early life history of two tuna species yellowfin tuna and Pacific blue tuna for the sustainable use of these resources Issue Sector Agriculture Cooperation scheme Technical Cooperation Projects Science and Forestry Fisheries Technology Division in charge Rural Total cost estimated at completion of the Project Development Dept 400 424thousand JPY Period of From April 1 Partner Country s Implementing Organization Cooperation 2011 to March Aquatic Resources Authority of Panama ARAP 31 2016 InterAmerican Tropical Tuna Commission Achotines La
37. v Do E IATTC Western Central Pacific Fisheries Commission WCPFC International Scientific ARRA S Committee for Tuna and Tuna like Species in the North Pacific Ocean ISC X 2 2
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81. DOE Mr Carlos E Zambrano AFA REA Technical Cooperation Advisor 3 M M MINUTES OF MEETINGS ON THE THIRD JOINT COORDINATING COMMITTEE MEETING FOR THE PROJECT FOR COMPARATIVE STUDIES OF THE REPRODUCTIVE BIOLOGY AND EARLY LIFE HISTORY OF TWO TUNA SPECIES FOR THE SUSTAINABLE USE OF THESE RESOURCES The Third Joint Coordinating Committee hereinafter referred to as JCC meeting was held on November 26 2013 at Hotel Continental in Panama City in conjunction with the Mid term Review Study of the Project The participants of the Third JCC meeting had a series of discussion and exchange of views on desirable measures to be taken for the Project and as a result of the discussions they agreed on the issues referred to in the Attached Document The participants are listed
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138. Licda SERRANO ON Secretaria General Laboratorio de Pesquer as Autoridad de los Recursos Acu ticos de Panam Universidad de Kinki UK ARAP i Sr Shunji S Ing Vefnon SCHOLEY L der Misi n Japonesa de Revisi n Director Intermedia Laboratorio Achotines Agencia de Cooperaci n Internacional del Comisi n Interamericana del At n Tropical Jap n JICA CIAT DOCUMENTO ADJUNTO 1 Aprobaci n del Informe El Equipo de Revisi n Intermedia que en lo sucesivo se denominar Equipo present el Informe de Revisi n Intermedia Conjunta que en lo sucesivo se denominar el Informe El CCC examin minuciosamente el contenido del Informe que aparece en el Ap ndice 2 y tom nota de las recomendaciones hechas en el Informe Se inst a que el personal contraparte de la Autoridad de los Recursos Acu ticos de Panam Comisi n Interamericana del At n Tropical CIAT y los investigadores de la Universidad de Kinki emprendan esfuerzos conjuntos para llevar a cabo las acciones necesarias para seguir las recomendaciones del Proyecto 2 Revisi n del Plan de Operaci n PO A fin de dar respuesta a la demora en la instalaci n de la jaula marina as como a la actual paralizaci n en el desove del at n de aleta amarilla se propuso una revisi n del PO la cual fue posteriormente aprobada El PO revisado se adjunta en el Ap ndice 3 3 Revisi n de la PDM El CCC aprob l
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140. 23 Maho Inomata Tomoki Honryo Yasuo Agawa Tokihiko Okada Toshio Tamura and Yoshifumi Sawada Research on handling technology improvement and stress response of juvenile Pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh Diciembre 2013 155 24 O Stein O Daniel Margulies OJeanne Wexler and O Vernon Scholey Comparative studies of feeding dynamics and growth of yellowfin tuna Thunnus albacares and Pacific bluefin tuna Thunnus orientalis larvae 2013 Tuna Conference Lake Arrowhead Calif May 2013 25 ODaniel Margulies O Vernon Scholey OJeanne Wexler and OMaria Stein Studies of tuna early life history conducted at IATTC s Achotines Laboratory 2012 2013 2013 Tuna Conference Lake Arrowhead Calif May 2013 26 O Vernon Scholey ODaniel Margulies OJeanne Wexler and OMaria Stein Studies of tuna early life history conducted at the Inter American Tropical Tuna Commission IATTC Achotines Laboratory 2012 2013 2013 Larval Fish Conference Miami FL Junio 2013 2 Presentaciones en las conferencias en Jap n 1 AYoshiki Tsutsumi Taro Matsumoto Tomoki Honrya Yasuo Yoshifumi Sawada Yasunori Ishibashi Impact of the various LED light source wavelength in illumination breeding on the breeding performance of juvenile bluefln tuna The 2011 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University Kyoto Univers
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146. 3 Aspecto Financiero El plan de asignaci n de presupuesto para el Proyecto fue acordado entre la ARAP la CIAT la JICA en primera reuni n de CCC Es importante cumplir con el acuerdo de asegurar los recursos financieros de una manera cooperativa 19 122 4 6 Conclusiones El Equipo de Revisi n Intermedia Conjunta ha confirmado que las actividades del proyecto en general han demostrado un buen avance En cuanto a los resultados de las actividades del proyeeto los conocimientos e informaciones cient ficas sobre el ciclo vital temprano la ecolog a de deshove y la nutrici n han sido producidos tal como se hab a planeado originalmente El resumen de la evaluaci n basada en los cinco criterios de evaluaci n se describe en la siguiente tabla Rawana aa Bhd Aa Eficiencia Moderadamente alta Se necesitan acciones correctivas para cubrir el tiempo perdido en la instalaci n de la jaula marina Se necesitan contramedidas para hacer frente a la paralizaci n actual en el desove Impacto Muy pronto para evaluar Se describe solamente impacto Sostenibilidad Probablemente ser Con la condici n de que el personal contraparte capacitado se mantenga en sus instituciones 5 Recomendaciones Temas relacionados con el procedimiento de implementaci n 1 Mejoramiento de la comunicaci n entre investigadores En el caso de este proyecto un gran n mero de investigadores d
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149. Japanese Expert long term 1 person project coordinator short term 18 researchers in total Trainees received in Japan 13 persons Provision of equipment around 927 thousand US dollars for ARAP and around 248 thousand US dollars for Japanese research institution Local cost expenditure around 260 thousand US dollars Counterpart organizations side Counterpart 19 persons in total at the mid term review Procurement of equipment around 58 thousand US dollars Local Cost around 750 thousand US dollars Provision of land and facilities office space for Japanese experts and facilities and equipment for experiments Evaluation Team Members of 1 Team Leader Mr Shunji Sugiyama Senior Advisor Japan International Evaluation Team Cooperation Agency JICA 2 Cooperation and Planning Mr Suguru Kubo Field Crop Based Farming Area Division Rural Development Department JICA 3 Evaluation JST Dr Makie Kokubun Professor Graduate School of Agriculture Division of Biological Resource Sciences Tohoku University 4 Evaluation JST Ms Chihiro Inoue Chief Research Partnership for Sustainable Development Division Japan Science and Technology Agency JST 5 Evaluation and Analysis Mr Isao Dojun Chuo Kaihatsu Corporation Period of From November 10 2013 to November 29 Type of Evaluation Mid term Evaluation 2013 Results of Evaluation 1 Achievement
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151. Toru Kobayashi Michio Kurata Tokihiko Okada and Yoshifumi Sawada Identification of a male characteristic DNA marker of pacific bluefin tuna Thunnus orientalis European Aquaulture 2012 Praque November 2012 15 Yasuo Agawa ATakafumi Komiya Tomoki Honryo Toru Kobayashi Michio Kurata Tokihiko Okada and Yoshifumi Sawada Identification of a male characteristic DNA marker of aquacultured pacific blucfin tuna Thunnus orientalis Aquaculture America 2013 Nashville February 2013 16 ODaniel Margulies O Vernon Scholey OJeanne Wexler OMaria Stein Yoshifumi Sawada Yang Su Kim Tomoki Honryo and O Angel Guillen Comparative research on the reproductive biology and early life history of Pacific bluefin tuna Thunnus orientalis and yellowfin tuna Thunnus albacares Aquaculture America 2013 Nashville TN February 2013 17 Amal Biswas Biswajit Biswas and Kenji Takii The development of formulated diet for promoting sustainable aquaoulture of Pacific bluefin tuna Thunnus orientalis BIT s 2nd Annual World Congress of Mariculture and Fisherles Hangzhou China September 23 235 2013 18 Yang Su Kim ATsukasa Sasaki AMaho Inomata Tomoki Honryo Yasuo Agawa Masashi Ando and Yoshifumi Sawada Effect of dietary taurine enhancement on the growth and ontogenetic development in red sea bream Pagrus major larvae Asia Pacific Aquaculture 2013 December 2013 19 Yasuo Agawa Yoshihiro Takada Kohei Tamura A
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154. transportation of materials The detailed breakdown of expenditures is provided in the Annex 8 3 1 2 Counterpart Side and ARAP 1 Counterpart personnel involved in the project activities At the time of the mid term review a total of nineteen 19 researchers and officials are involved in the project activities among which 13 persons are from ARAP and 6 persons are from IATTC Researchers are organized in three thematic groups as described in the table below Kinki Universit Spawning ecology 13 development Group Early life history and rearing technology development It was reported that six researchers of resigned for various reasons who were immediately replaced by new counterparts The detailed list of counterpart personnel 5 provided in the Annex 9 Three researchers of IATTC headquarters have visited the Achotines Laboratory twice a year for catrying out experiments related to YFT They also visited the Kinki University once a year around 30 days per visit for carrying out experiment related to PBF 2 Equipment provided by counterpart organization ARAP has provided such equipment as laptop computer outboard motor refrigerator and air conditioner The total amount of the equipment purchased was 8 536 US dollars The detailed list of equipment is provided in the Annex 10 3 Project Operation Cost Allocated by the Counterpart Organizations Expenses for such items as fu
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157. 3 Achievement of the Project 3 1 Inputs 3 2 Progress of the Planned Activities 3 3 Outputs 3 4 Project Purpose 3 5 Project Implementation Structure and Project Implementation Process 4 Results of Review 4 1 Relevance 4 2 Effectiveness 4 3 Efficiency 4 4 Impact 4 5 Sustainability 4 6 Conclusions 5 Recommendations Annexes Annex 1 Schedule of the Review Annex 2 Project Design Matrix version 0 1 Annex 3 List of Japanese Researchers Involved in the Project Activities Annex 4 Dispatch of Japanese Experts Researchers 5 Counterpart Personnel Trained in Japan Annex 6 Equipment Provided by Japanese Side for the Achotines Laboratory Annex 7 Equipment Procured for the Research Institute in Japan Kinki University Annex 8 Local Cost Allocated by Japanese Side Annex 9 List of Counterpart Personnel Involved in the Project Activities Annex 10 Equipment Procured by the Counterpart Organization Annex 11 Local Cost Allocated by the Counterpart Organizations Annex 12 List of Publications Annex 13 List of presentations in the international conferences and conferences in Japan Abbreviations and Acronyms Aquatic Resources Authority of Panama Inter American Tropical Tuna Commission JC Joint Coordinating Committee JICA Japan International Cooperation Agency JT Japan Science and Technology Agency JST MEF Ministry of Economy and Finance Pacific Bluefin tuna PDM Project Des
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161. The 2013 spring meeting of the Japanese Socicty of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 7 Gentoku Nakase Tomoki Cano Daniel Margulies Yoshifumi Sawada Improvement of yellowfin larvae initial survival by Nanokuroropushisu prior addition The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 156 8 ATakayuki Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Studies on the behavior and feeding on bluefin tuna larvaey effects of water temperature and food on the swimming speed The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 9 ATakayuki Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Bffects of feeding on oxygen consumption and swimming velovity of Bluefin tuna larvae The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 10 Michio Kurata Yasunori Ishibashi Tomoki Shigekazu Katayama Hiromu Fukuda Kenji Shigeru Miyashita Hidemi Kumai Yoshifumi Sawada Dysfunction of swimbladder inflation of Bluefin tuna relationship with growth and lordosis The 2013 autumn meeting of the Japanese Society of Fisheries Science Mie University Septiembre 2013 3
162. When person to person communication among researchers fails Research Coordinators can also step in to facilitate better communication 3 Information sharing among researchers In this Project researchers are grouped in three thematic areas and there is a tendency that information flow is rather confined within the same research group n order to facilitate inter and intra group information sharing and exchange it is recommended that information sharing events such as in house seminars workshops be organized In these events research plan findings and related technologies can be presented Such events are also good opportunities for visiting researchers to introduce themselves to other parties 4 Advanced planning of research activities Certain research works require sufficient lead time for preparation e g propagation of live feed and ARAP researchers need to obtain a travel authorization to visit the Achotines Laboratory 30 days prior to their departure These facts together indicate the importance of advanced planning of research activities in this project Furthermore in order to overcome language barriers and facilitate communication documentation of research plen schedule is highly encouraged Documented information can serve as a good basis for discussion and consultation among researchers of different organizations It is therefore requested that all the researchers prepare a research plan schedule in a written form well in adva
163. and nutrition have been produced as originally planned The summary of evaluation based on five evaluation criteria is described in the table below Criteria Evaluation Remarks Relevance High Effectiveness High Efficiency Moderately high Corrective actions are needed to cover lost time in ocean cage installation Countermeasures are needed to deal with current halt in spawning Impact Premature to assess Only provisional impact 15 described Sustainability Likely to be high On the condition that trained counterpart personnel remain in the organizations 6 Recommendations A Issues relating to the implementation procedure 1 Improved communication among researchers A large number of researchers from three different counterpart organizations ARAP and Kinki University are involved to conduct joint research and many researchers visit the Achotines Laboratory on a short term basis E mail is main tool for communication when the researchers are not in the Achotines Laboratory and the efficiency of communication depends largely on communication skills ability of individual researchers and language barriers also exist It is necessary to exert upmost efforts to improve communication XV 2 Official assignment of research coordinators Assign a research coordinator at each counterpart organization for a centralized single channel for organization to organization
164. primavera de 2014 Si los reproductores de YFT del Laboratorio reanudan el desove para la primavera de 2014 es posible que se completen las actividades de investigaci n mediante la jaula marina en los dos a os restantes Indicador 4 6 Descripci n del desarrollo del rgano visceral de larva y juvenil de YFT Estaba previsto iniciar una investigaci n sobre el desarrollo de los rganos internos u rganos de v sceras con larvas y juveniles producidos en 2013 Sin embargo el desove de los reproductores de del Laboratorio Achotines se detuvo en 2013 Por lo tanto no se obtuvierou las muestras necesarias para la investigaci n Se pondr en marcha esta investigaci n despu s de la reanudaci n del desove El tiempo necesario para llevar cabo esta investigaci n es de alrededor de un por lo tanto se espera que la descripci n del desarrollo de los rganos viscerales con este indicador se complete durante el periodo del proyecto 3 4 Prop sito del Proyecto Prop sito dei Proyecto Se obtiene y sintetiza adecuadamente el conocimiento cient fico y la informaci n sobre la biolog a reproductiva y el ciclo vital temprano de dos especies de at n aleta amarilla YET y aleta azul del Pac fico PBF los cuales son fundamentales para el uso sostenible de estos recursos Indicador 1 S ntesis de la informaci n biol gica aplicable al manejo del recursos de especies atuneras el cual se difund
165. spawning ecology study of YFT nutrition requirements study and development of YFT diet etc For further details refer to the Annex 4 3 Counterpart personnel trained in Japan Thirteen 13 counterpart personnel have participated in the trainings in Japan Themes of training were early growth and survival of tuna species survival of tuna at larval and juvenile stages fish diseases and molecular biology For further details refer to the Annex 5 4 Provision of Equipment 1 Equipment provided by the Japanese side to the Achotines Laboratory Equipment for research activities and fish culture which amount in total is around 927 thousand US dollars has been provided by JICA The detailed list of equipment is provided in the Annex 6 44 lt ARA 203 959 00 2 Equipment provided to the research institute in Japan Equipment for research activities which amount total is around 24 8 million Yen The detailed Hist of equipment is provided in the 7 5 Local cost borne by Japanese side Local cost in Panama borne by the Japanese side for the implementation of project activities is around 262 thousand US dollar in total as of September 2013 This includes the expenses for the extension of the main building for additional meeting room office and storage of equipment maintenance of equipment procurement of equipment purchase of consumables contracted maintenance of equipment
166. 14 15 4 O Daniel Margulies O Vernon P Scholey O Jeanne Wexler O Maria Stein Achotines Laboratory Home To Continuing Studies Of Tuna Early Life History the Global Magazine for Parmed Seafood March April 2013 pp 72 73 5 ODaniel Margulies O Vernon P Scholey OJeanne B Wexler OMaria 8 Stein Richard Deriso and Guillermo A Compean The IATTC Achotines Laboratory a world leader in tuna research INFOFISH International 2013 pp 24 28 6 OVernon P Scholey ODaniel Margulies OMaria 5 Stein Guillermo A Compean OJeanne Wexler Richard Deriso Cr a de atunes El laboratorio de la CIAT en Achotines Panam INFOPESCA INTERNACIONAL N MERO 52 OCTUBRE DICIEMBRE A O 2012 26 29 7 ODaniel Margulies OVernon Scholey OJeanne Wexler and OMaria Stein Achotines Laboratory home to continuing studies of tuna early life history Global Aquaculture Advocate March April 2013 72 73 8 OMaria Stein O Daniel Margulies O Vernon Scholey and OJeanne Wexler El Laboratorio de Achotines atunes aleta amarilla cautivos en Panam Panorama Acu cola 18 3 26 32 9 OJeanne Wexler ODaniel Margulies O Vernon Scholey and O Maria Stein El Laboratorio de Achotines Panama Fishing Magazine March 29 2013 6 8 10 O Daniel Margulies O Vernon Scholey O Jeanne Wexler O Maria Stein Richard Deriso and Guillermo Compe n Cr a de atunes el laboratorio de la CIAT en Achotines Panam INFOPESCA Internacional 52 26 2
167. 5 Countermeasures to the halt in spawning of Yellowfin tuna JCC discussed the countermeasures to the current halt in spawning of YFT and agreed to consider initiating preparatory work for possible hormonal injection as a practical option to induce spawning in a small group of broodstock held in a reserve tank Additional options will continue to be explored 6 Other matters 1 In order to ensure financial stability of the Project it is reitcrated that ARAP IATTC and JICA shall be adhered to the agreement made at JCC meetings 2 IATTC made a request to JST JICA to explore the possibility of providing financial assistance to the JATTC international staff members travel to Japan to participate in the research work at the Fishery Laboratory of the Kinki University and to periodically attend an important international scientific meeting This request was made since the IATTC counterparts do not currently have project related financial support for this type of travel END Appendixl List of participants Appendix2 Joint Mid Term Review Report Appendix3 Plan of Operation Version 2 lt Appendix 1 LIST OF PARTICIPANTS LUNCH MEETING JOINT COORDINATING COMMITTE AND MID TERM REVIEW OF THE PRO ATUN PROJECT Date November 26 2013 Time 9 00 a m 2 30 p m Place Hotel Continental Panama City Nely Serrano ARAP Secretaria General Era alin KY Beh ARAP Director General de Investigaci n y D
168. 60 210271040 A30 01q AB NDIJOJA 74 1102 81 60 1100 22 90 ES omes T ied I MO poures jouuosg9g 3434493102 xouuy 5910995 3o TEATAS 0J uo 3uturen 12914291 UPA 5202494 JO Aog Sg9Jnose tino pry PY 10121008 SASIA 0001915 511450 pue 4 Jo Arion 590052 DHENSY uupugd jo Anoqny 58211059 JO AMO NY 5331059 Y 3101127102320 AISAN AGEIOqPT SS uonejs e 028461 01686 oao taape 72111102 sunousy wg CCo WEGE 2111192 semibuov ewened Tinio supy sa moanf PP SCALE UBT 20 15poaj meer focor Sasjoumon od vede dubi seu madsen onies xum oqunro ques 1 0001 TRAYS 9 XALANTO orog minsolonr 10 aayos SusAry weder o ST si A MM A ur uopemps gt ANQNUEND YNA YNA Cowan tt 4 0002 GN LCN 000zdompoueN smesatle stss erpeds weder st
169. 91 sis amp peue spree 209 5 opo juoudgr StL ario dl lt 8 8 6 144 ames am 2822 06250 Jo 195 mder Vgjarmbpe suene S 2 osed 0120 810007 TrSO ep IP umynousy ECEOZTOT 91502102 2011104 ue 6196 lt 1 619661 02293 9pr3neT 002 91 0417502 02602 1950 fpe OGUg BRA d 32099593014 PRPISIOAINN 5594 otrolpzoqt op peprszoAmu 29594 BUNYSO UTIRISH HUY Pepisiatur osag ap 01301210481 PUNISO 10198158 HUTA 15 25524 pens unadx 1019853 9594 OIJOJ OB ugroujsq PEPISISATU 22594 T uorovisq PSpISISAIU 22694 meinn ap 19128151 25524 2 OU0 810qE T
170. Annex 9 IATTC 3 2 1 30 2 ARAP 580 3 M M Annex 10 3 ARAP IATTC 1 11 y T JCC E 3 M M Annex
171. Growth and lipoprotein lipase 2 and PPAR gene expression in juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 3 Kim Y S Aoki T Yamamoto S Ishimoto K Murata O Sawada Y Effect of starvation on survival rate and body composition of juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 4 Yang Su Kim ODelgado D S OScholey V Yoshifumi Sawada Studics on proper brccding water of yellowfin tuna larvae in embryonic development and early larvae stages 2012 spring meeting of the Japanese Society of Fisheries Science National Fisheries University 2012 5 Tomoki Honryo OGuillen A Olbarra OMargulies D OScholey V Toru Kobayashi Yoshifumi Sawada Effect of water temperature on yellowfin tuna egg generation speed and hatching time The 2012 spring meeting of the Japanese Society of Fisheries Science National Fisheries University March 2012 6 Yasunori Ishibashi A Yoshiki Tsutsumi Taro Matsumoto Tomoki Honryo Yoshifumi Sawada and Yasuo Agawa Effects of photoenvironmental control on the feeding growth and survival rate of juvenile Pacific bluefin tuna Thunnus orientalis Asia Pacific Marine Biotechnology Conference APMBC Kochi July 2012 7 AShinichiro Hashimoto Tomoki Honryo Yasuo Agawa Yoshifumi Sawada Taro Matsumoto and Yasunori Ishibashi Determination of the optimal light wave
172. Jo uononpoud 10 ser o ouqoo 2822 pue Ayay Jo jusuido o sq 420 5 LIA 20 spomeul uoreyodsuen pue amde TO LAA JO 3ueureseueur 10 Jo OT O LAA JO WomaSeueu pue srsA Eue 10 51001 Jo jueurdo oAe T LIA Jo sed empu pue IPIOTHTB jo Euonrnnu aq jo 2 53815 184 PUE LAA JO RATAMS pue paoi ASojoog Surpas Jo OnBSnSaAut LAA ur uoreuxogur 1431 ol suods i JEAIE pue SONSLISIIEIBYO UOISIA am JO Add pue L A uo 510129 Teoruayo pue TeorsKqd Jo ap pue w Jo SOUT s dures LAA igne prm panojas Aq JO LAA 0 e se doo T JO 8 02810 JO Aexreoxorar pue WNC pue LAA Jo 5 50 Jo snyeys e2tS0 ors amp qd o qano1om hd e durrs e jo jo Surumeds smels guonrnnu Jo JO SuJumeds T
173. Licdo Osvaldo Alexis Silva T a 06 Tropical en Panam 1 4 Programa de la Revisi n Intermedia El Programa de la Revisi n Intermedia se llev a cabo del 11 de noviembre al 26 de noviembre de 2013 como se muestra en el Anexo 1 4 Evaluaci n IST Chihiro Inoue 1 4 1 An lisis Inicial en Jap n El Equipo Japon s de Revisi n Intermedia revis 105 documentos disponibles relacionados con el Proyecto incluyendo informes de proyectos cuestionarios contestados por los investigadores y expertos japoneses y personal contraparte en Panam y prepar una tabla de evaluaci n que enumeraba los puntos de revisi n espec ficos y los m todos de recolecci n de datos 1 4 2 Actividades de revisi n en la Rep blica de Panam El Equipo Conjunto de Revisi n Intermedia visit la ARAP y el Laboratorio de Achotines y lleyaron a cabo una serie de entrevistas y conversaciones con el personal de contraparte en Panam y con los investigadores y expertos Japoneses ete 1 5 Metodolog a la Revisi n Intermedia 1 5 1 M todo de la Revisi n El Proyecto fue revisado conjuntamente por el Equipo bas ndose en los materiales que muestran el marco del Proyecto tal como PDM PO y el Registro de Discusiones R D El trabajo de revisi n consiste en el an lisis de los informes del proyecto cuadernos diarios de operaci n de los equipos estudios de campo y entrevistas con los investigadores de
174. Output 1 Spawning characteristics of YFT and PBF are determined Achievement As for identification of optimum nutritional condition of YFT broodstock it was suggested that the use of feed with higher polyunsaturated fatty acid HUFA is necessary for broodstock of YFT As for identification of optimum environmental factors for YFT spawning the temperature range of spawning under the captive condition is found to be more than 24 Development of biochemical assay method for determining egg quality of YFT will be started after resume of spawning of YFT broodstock Output 2 The method to identify maternal line of YFT is established by using mitochondria D loop for analysis Achievement Primers to detect the polymorphism of mitochondrial D loop were identified The results of analysis indicated that genetic diversity was wide but dominant populations were not found However in order to verify these results other methodologies will be employed Output 3 Critical factors that affect survival of YFT and PBF in their early life history are identified Achievement Microscopic and histological observation of YFT embryogenesis was completed and its analysis is underway The investigation of effects on the progress of morphogenesis hatching rate rate of normal larvae and daily number of death fish were conducted and comparison with data on PBF is under way The research of external and internal morphological development
175. be employed 3 3 3 Output 3 Critical factors that affect survival of YFT and PBF in their early life history are identified Indicator 3 1 Developmental speed and embryogenesis process of YFT and PBF described in relation to physical and chemical factors Microscopic and histological observation of YFT embryogenesis was completed and its analysis is underway As for effects of salinity and water temperature on the developing speed hatching rate survival rate and the investigation of effects on the progress of morphogenesis hatching rate rate of normal larvae and daily number of death fish were conducted Comparison with data on PBF is under way In summary the experiments and analysis related with this indicator have been completed therefore this component has been achieved LE H ids indicator 3 2 External and internal morphological development of larval and juvenile YFT and PBF described The research of external and internal morphological development of PBF larvae was completed As for YFT the collection of samples of external morphology has been completed Sample collection for intemal morphologies of larvae and external internal morphologies of YFT juveniles and young fishes and analysis will be carried out in future Indicator 3 3 Similarities and differences identified in vision characteristics and the larval respanse to light information in YFT and PBF feeding ecology behavior growth and survival o
176. be carried out in the later stage At this point the temperature range of spawning under the captive condition is found to be more than 24 C YET spawning was put on hold in autumn 2012 when a red tide occurred in the area near the Achotines Laboratory following heavy rain in the rainy season From this event it becomes clear that changes in water quality and salinity would affect spawning of YFT fn addition it was found that light intensity and photoperiod daylength are not the factors to affect spawning As to the relationship between spawning and moon phase additional data need to be collected In summary the effects of three environmental factors temperature range photoperiod and light intensity have been identified and study of additional environmental factors will be carried out Indicator 1 3 Biochemical assay developed to determine egg quality of YFT and PBF As for visceral tissue sample collection was carried out to clone the physiological situation related to lt 10 genes in the internal organ As for YFT samples were collected from 20 wild YFT which were caught Okinawa Japan Analyses on the change of egg nutrition component and various enzyme activities associated with egg development of YFT and PBF were conducted The above work indicated similarity of nutritional and enzyme activity change during the egg development period between and PBF This suggests metabolic process where
177. buena base para el manejo efectivo de dichos recursos atuneros 2 Relevancia en las politicas nacionales pertinentes de Panam La Pol tica sobre los Recursos Acu ticos de Panam para la Pesca y Acuicultura 2010 establece catorce principios b sicos que incluyen la promoci n del manejo sostenible de recursos marinos y la promoci n de investigaci n y desarrollo de tecnolog a adecuada de pesca y acuicultura para desarrollar la pesca y acuicultura responsables La Estrategia Nacional para el desarrollo de acuicultura 2012 describe la 1 Comisi n de Pesca dal Pacifico Occidental y Central WCPFC en ingl s 2 Comit Cient fico Internacional sobre Atunes y Especies Afines en el Oc ano Pac fico Norte ISC en ingl s 16 119 importancia del desarrollo de la acuicultura en armon a con el manejo ambiental y tambi n espec ficamente promueve la investigaci n de YFT debido a su elevado potencial Se puede decir que el objetivo del Proyecto coincide con los objetivos de las pol ticas paname as antes mencionadas 3 Concordancia con la pol tica de asistencia de Jap n hacia Panam Una de los sectores importantes del trabajo de asistencia de JICA en Panam es la conservaci n ambiental la cual naturalmente incluye la conservaci n del ambiente natural y de los recursos biol gicos Se prev que este Proyecto contribuya a fortalecer el manejo de recursos de especies de at n la cual est dentro del rea de
178. communication The research coordinators take a lead role in organizing research activities within their organizations and discuss consult with the research coordinator of other organizations When person to person communication among researchers fails research coordinators can also step in to facilitate better communication 3 Information sharing among researchers Researchers are grouped in three thematic areas and there is a tendency that information flow is rather confined within the same research group In order to facilitate inter and intra group information sharing and exchange it is recommended that information sharing events such as in house seminars workshops be organized 4 Advanced planning of research activities ARAP researchers need to obtain a travel authorization to visit the Achotines Laboratory 30 days prior to their departure Certain research works require sufficient lead time for preparation such as propagation of live feed It is necessary to share information on research activities at the Achotines Laboratory among persons concerned well in advance of their visit to the Laboratory Research plan schedule in a written form should be shared among persons concerned and research coordinators 5 Improvement of counterpart training in Japan The importance of advanced planning and documentation of information for consultative implementation is also applicable to counterpart training in Japan Close consultati
179. critical input are indispensable and hence it should be ensured that existing ARAP counterparts continue to take part in the Project for the remaining period of the project 8 Publicity of the Project The presence and significance of the Project needs to be widely disseminated to the general public of Panama and Japan so as to facilitate their understanding of the Project as well as to harness their support for project activities For this purpose it is recommended that the Project prepare and disseminate informative materials such as brochure and newsletters that promote understanding of the outline of the Project objective expected outputs and major activities Considering the fact that tuna resources are important shared resources in the Pacific Ocean which requires regional efforts for management it can be said that the scientific findings of the Project are also valuable scts of information in regional terms In view of the above it is advisable that all the parties concerned of the Project consider organizing an international regional seminar to disseminate the project results to wider audiences 9 Financial contribution In order to ensure financial stability of the Project it is urged that IATTC and be adhered to the agreement made at JCC meetings 10 Improved management and security of equipment In this project a variety of technical equipment and instruments are provided which are utilized by multiple user
180. cultivo en jaula para producci n de alevines de YFT 4 5 Investigaci n del desarrollo de los rganos viscerales y sus funciones y la calidad y cantidad de alimentos para YFT 5 Instituciones Responsables Parte Japonesa Universidad de Kinki i Parte Paname a Autoridad de Recursos Acu ticos de Panam y Laboratorio Achotines CIAT 6 Per odo del Proyecto Desde I de abril de 2011 hasta 31 de marzo de 2016 5 a os 3 Logros del Proyecto 3 1 Insumos 3 1 1 Parte Japonesa 1 Investigadores japoneses involucrados en las actividades del Proyecto Las actividades de investigaci n del Proyecto han sido a cabo en Panam y Jap n n mero de investigadores que han participado en las actividades de investigaci n en Jap n es de 36 en total en la 107 Universidad de Kinki incluyendo estudiantes de licenciatura Entre ellos 18 investigadores participaron en las actividades de investigaci n tanto en Jap n como en Panam Para m s detalles v ase el Anexo 3 0 Env o de Expertos e Investigadores Japoneses Se han enviado a Panam un experto de largo plazo Coordinador del Proyecto y 18 investigadores de corto plazo en los campos de desarrollo de tecnolog as de cultivo de larvas estudio del ciclo vital temprano estudio de ecolog a de desove de YFT estudio de requerimientos de nutrici n y desarrollo de dieta de YFT etc Para m s detalles v ase el Anexo 4 3 Personal Contraparte Capa
181. de peces Estaba previsto inicialmente investigar los efectos de la cantidad y frecuencia de alimentaci n y aditivos Sin embargo las actividades para la identificaci n de la condici n nutricional ptima para los reproductores han sido suspendidas debido a que el cambio de las condiciones de alimentaci n puede causar una disminuci n en la producci n de huevos dado que s lo una de reproductores est disponible y puede igualmente afectar negativamente las otras actividades de investigaci n Existe una situaci n dif cil para llevar a cabo la investigaci n sobre el desarroilo de la alimentaci n con f rmula para reproductores ya que es necesario iniciar el transporte de los ingredientes para la preparaci n de la alimentaci n con f rmula Aunque existe un factor limitante s lo hay una l nea de reproductores disponible el equipo de investigadores prev que el consumo de alimentos vivos a los cuales se le ha a adido fosfol pidos DHA vitamina E y vitamina C tendr efectos positivos sobre la inducci n de desove y la calidad del huevo se ha investigado bien de que este m todo aumenta el efecto sobre la inducci n del desove y calidad del huevo para otros tipos de peces Indicador 1 2 Al menos dos factores ambientales ptimos identificados mbito de temperatura intensidad de luz fotoperiodo fase lunar qu mica del agua para el desove de YFT El desove fecundidad poca de desove eclosi n y las con
182. del laboratorio En cuanto a los investigadores se necesitan hacer arreglos de giras de trabajo ya que la mayor a de ellos necesitan viajar al Laboratorio desde sus puestos de trabajo Siendo as se necesitan por lo menos 30 d as de tiempo de espera para la solicitud y preparaci n de sus giras de trabajo Se ha informado de que ha habido un n mero de casos donde no se ha otorgado suficiente tiempo antes de la realizaci n de actividades conjuntas de investigaci n 4 Resultados de la Revisi n 4 1 Relevancia f Se considera que la relevancia del Proyecto es alta en base a los hechos presentados a continuaci n 1 Necesidad de mejorar el manejo de los recursos de especies de at n La pesquer a atunera es una industria importante para los pa ses centroamericanos incluyendo Panam Panam exporta una cantidad considerable de at n congelado y fresco el cual constituye una parte importante de los ingresos de divisas Sin embargo la tendencia decreciente de la captura de at n es evidente en el Oc ano Pacifico y las organizaciones regionales involucradas con la investigaci n y manejo de los recursos de at n tales como la CIAT WCPFC1 y ISC2 han instado a tomar medidas urgentes de conservaci n y manejo El objetivo de este Proyecto es de obtener y sintetizar el conocimiento e informaci n cient fica sobre la biolog a reproductiva y el ciclo vital temprano de dos especies de at n y se espera que sus resultados formar n una
183. expression of Pacific bluefin tuna Thunnus orientalis juvenile Temminck and Schlegel Submitted to Aquaculiure Research 7 Yoshifumi Sawada Tomoki Honryo Yasuo Agawa Yang Su Kim Shigeru Katayama Michio Kurata Tokihiko Okada Shigeru Miyashita Teratogenic effect of short term hypercapnia and hypoxia on red sea bream Pagrus major embryos Submitted to Journal of Fish Biology 8 Yang Su Kim ODaniel Margulies O Vernon P Scholey O Amado Cano and Yoshifumi Sawada The effect of temperature and salinity on hatching rate and larval survival rate of yellowfin tuna Thunnus albacares Submitted to Aquaculture 9 Yang Su Kim OTsukasa Sasaki Awa OMaho Inomata Tomoki Honryo Yasuo Agawa Masashi Ando and Yoshifumi Sawada Effect of dietary taurine enhancement on the growth and ontogenetic development in red sea bream Pagrus major larvae Submitted to Aquaculture Research 10 Yasuo Agawa Takafumi Komiya Kouhei Tamura Tomoki Honryo Tsukasa Okada Naoki Yagishita Toru Kobayashi and Yoshifumi Sawada Identification of Male and Female Sex Linked DNA Sequence of Cultured Pacific Bluefin Tuna Thunnus orientalis Submitted to Aquaculture Research a 11 O Amado Cano Yang Su Kim Darys Delgado Vernon Scholey Yoshifiuni Sawada Comparative efficacy of anesthetics among MS 222 2 phenoxyethanol and clove oil in yellowfin tuna Thunnus albacares early Juveniles Submitted to Aquaculture Science 12 Teruyoshi Ta
184. in Appendix 1 of the Attached Document The Minutes of Meeting are written both in English and Spanish versions with each text being equally authentic Panama City 26 November 2013 Ms Nely SER baratoi Secretary General Aquatic Resources Authority of Panama Kinki University KU 4 y ARAP p L Mr Vernon SCHOLEY Director Achotines Laboratory Inter American Tropical Tuna Commission IATTO ATTACHMENT 1 Adoption of the Report The Mid term Review Team hereinafter referred to as the Team presented the Joint Mid term Review Report hereinafter referred to as the Report JCC examined thoroughly the contents of the Report in the Appendix 2 and took note of the recommendations made in the Report It was urged that counterpart personnel of the Aquatic Resources Authority of Panama ARAP Inter American Tropical Tuna Committee IATTC and Kinki University Researchers make joint efforts to take necessary actions to follow up recommendations of the Project 2 Revision of Plan of Operation PO In order to respond to the delay in installation of ocean cage as well as to the current halt in spawning of Yellowfin tuna a revision of PO was proposed which was subsequently approved by JCC the revised PO is attached in Appendix 3 3 Revision of PDM JCC approved technical corrections made on PDM as described in the table below Indicator for Project Synthesis of biological Syn
185. invesdzadores MOU Experto dalerg plara 2 Coordinedor de Proyecto AU investigadores en EEUU 3 Investigadores en d Labomiorio Achotines Investigaci n en Jap n Capecitaci n en Jap n Investigeci n Conjunta y Frensezencia ta Panama Laboratorio chotines sitio prinripal en Panama Las reuniones del Comit Conjunto de Coordinaci n CCC se han celebrado dos veces y en estas reuniones se llegaron a acuerdos sobre los temas del programa que se indican a continuaci n Aporte presupuestario al Proyecto de parte de cada parte Selecci n de la contraparte par la capacitaci n en Tap n Plan de trabajo anual de las actividades del Proyecto Asipnaci n del personal contraparte del Proyecto Directrices para el uso de los equipos del Proyecto 3105 de Investigaci n y especia objetivo Bas ndose en los factores antes mencionados se puede decir que las reuniones de CCC fueron eficaces en la discusi n de 105 temas y en la toma de decisiones colectivas Adem s se establecieron cuatro subcomit s del Proyecto comit de direcci n comit de investigaci n comit de presupuesto y comit de relaciones p blicas y se asignaron varias personas de tres organizaciones en cada subcomit Los miembros de los subcomit s se comunican principalmente a trav s de correo electr nico sin embargo a veces la com
186. la ARAP y del Laboratorio y con 105 105 investigadores expertos japoneses Por otra parte se llev a cabo un examen exhaustivo de toda la informaci n pertinente obtenida mediante la aplicaci n de los siguientes cinco criterios de evaluaci n 1 5 2 Criterios de Evaluaci n Cinco Criterios de Evaluaci n 0 Relevancia La relevancia se refiere a la validez del Prop sito del Proyecto y ja Meta Superior en relaci n con la pol tica de desarrollo de las autoridades competentes de Panam as como las necesidades de los beneficiarios y la pol tica de ayuda de Jap n 0 Efectividad La efectividad se refiere a la medida en que los beneficios esperados del Proyecto se han cumplido seg n lo previsto Tambi n examina si estos beneficios se han producido como consecuencia del Proyecto 3 Eficiencia La eficiencia se refiere a la productividad del proceso de implementaci n Examina 51 105 insumos del proyecto se han convertido en resultados de manera eficiente 4 Impacto El impacto se refiere a los impactos directos e indirectos positivos y negativos causados por la implementaci n del Proyecto incluyendo el grado en que se ha alcanzado la meta superior 5 Sostenibilidad La sostenibilidad se refiere a la medida en que el Proyecto puede ser desarrollado a n m s por las autoridades competentes de Panam y el grado en que los beneficios generados por el Proyecto pueden sostenerse dentro de las pol tica
187. muestras se obtuvieron de 20 YFT silvestres que fueron capturados en Okinawa Jap n Se llevaron a cabo an lisis sobre el cambio del componente de nutrici n del huevo y diversas actividades enzim ticas asociadas con el desarrollo del huevo de YFT y PBF El trabajo antes mencionado indic una similitud en cuanto al cambio de la actividad nutricional y enzim tica durante el per odo de desarrollo de los huevos entre YFT y Esto sugiere un proceso metab lico donde los l pidos se consumen principalmente como fuente de energ a para el desarrollo de los huevos mientras que el consumo de prote nas se mantiene en lo m nimo A partir de estos hallazgos se propuso que un indicador importante para la determinaci n de la calidad de los huevos es el lipido de huevo El desarrollo del m todo de ensayo bioqu mico para determinar la calidad del huevo de se iniciar despu s de la reanudaci n del desove de reproductores de YFT y se prev que un m todo de ensayo bioqu mico se desarrolle dentro del per odo del Proyecto 3 3 2 Resultado 2 Se establece cl m todo para identificar las l neas maternas de YET a trav s del uso de D loop mitocondrial en el an lisis Indicador 2 1 Primers identificados para detectar el polimorfismo de D loop mitocondrial En cuanto a YFT se recogieron aletas de YFT en el mercado de pescado cerca del Laboratorio Achotines se tomaron las muestras de esas aletas y se llevaron a cabo los an li
188. of PBF larvae was completed As for the collection of samples of external morphology has been completed As for visual features of larvae of YFT and their similarity was confirmed as a kind of opsin gene sequence is developed largely at the start of feeding It has been suggested that factors inducing cannibalism in larvae of YFT are the difference of size and lack of feeding On this aspect similarity with PBF is confirmed It was found that the fortification of rotifer is effective for YFT as feeding material at the early growth stage Output 4 Fingering production technologies that support early life history study of YFT are developed Achievement As for YFT BAC using DNA extracted from liver was developed There was no parasitic infection which seriously affects the health of the YFT broodstock In order to improve methods for capture and transportation of YFT body temperature changes in spine during transportation have been investigated and it is suggested that stress of YFT continues for several hours after capture Experiments on rearing juveniles in ocean cage and investigation on development of internal organs or viscera organs will be started after the resume of spawning xi Project Purpose Scientific knowledge and information on the reproductive biology and early life history of two tuna species which are fundamental for the sustainable use of these resources are adequately obtained and synthes
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190. proyecto 1 3 Miembros del Equipo de Revisi n Conjunta El Proyecto fue revisado por el Equipo Conjunto de Revisi n Intermedia en adelante denominado el Equipo compuesto por personal de la parte japonesa y las contrapartes en Panam El equipo estaba compuesto por cinco 5 miembros de la parte japonesa y tres 3 miembros de las autoridades paname as y CIAT 104 OG ri 1 Equipo Japon s de Revisi n TAPO T Lid Sr Shunii 8 S nior Agencia de Cooperaci n Internacional del er r Shunji Sugiyama ji Jap n JICA Planificaci n de Divisi n de reas Agr colas de Cultivos en General 2 Sr Suguru Kubo la Cooperaci n Departamento de Desarrollo Rural JICA rofesor Escuela de Postgrado de la Divisi n Agr cola d 3 Evaluaci n Kokubun d bs I ee ES Ciencias de Reeursos Biol gicos Universidad de Tohoku Jefe Divisi n de Asociaci n de Investigaci n para el Desarrollo Sostenible Agencia de Ciencia y Tecnolog a de Jap n JST Evaluaci n 5 y Sr Isao Dojun Consultor Chuo Kaihatsu Corporation An lisis 1 3 2 Equipo de Revisi n Intermedia de las Instituciones aa Director SR de la Autoridad de Recursos Acu ticos L d icdo Edison ia de Panam ARAP en Los Santos Coord de TU Lourdes HER gor o e Cooperaci n T cnica Ministerio de Econom a y Finanzas MEF Representante Comisi n i At n 3 Miembro
191. research and investigation World Aquaculture Society World Aquaculture Magazine 12 Tomoki Honryo International research and tuna farming Wakayama version of the Sankei Sinbun a Japanese newspaper 27 July 2013 Annex 13 List of presentations in the international conferences and conferences in Japan 1 Presentations in the international symposiums and conferences 1 Yoshifumi Sawsada Aquaculture technology for Pacific bluefin tuna life cycle completion Symposium of Taiwan and Japan Aquaculture Engineering on the Environment Balance Management and Technology October 2010 National Talwan University 2 Yoshifumi Sawada PRESENT STATUS OF TECHNOLOGY AND ITS PRACTICAL APPLICATION IN TUNA AQUACULTURE AND RESTOCKING IN JAPAN Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 3 Y Sawada Comparative Studies of the Reproductive Biology and Early Life History of Two Tuna Species for the Sustainable Use of these Resources SATREPS Kick Off Symposium 17th Nov 2011 Panama City 4 8 Miyashita Representative Research Institute Kinki University SATREPS Kick Off Symposium 17th Nov 2011 Panama City 5 T Kobayashi Giving the research content outline of the Spawning Ecology Team SATREPS Kick Off Symposium 17th Nov 2011 Panama City 6 Giving the research content outline of the Nutrition and Feed Development Team SATREPS Kick Off Symposium 17th Nov 2011 Panama City 7 Y Ishibashi Giving the
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193. the nutritional value of artificial and natural food types of YFT and PBF 4 1 Development of tools for genetic analysis and management of YFT 4 2 Collection of information for health management of YFT 4 3 Improvement of capture and transportation methods for brood stock candidates 4 4 Development of hatchery and cage culture technologies for fingerling production of YFT 4 5 Investigation of the development of visceral organs and their functions and the quality and quantity of feeds for YFT 5 Responsible organizations Japanese side The Kinki University Panamanian side Aquatic Resources Authority of Panama ARAP and Achotines Laboratory 1ATTC 6 Project Period From April 1 2011 to March 31 2016 5 years 3 Achievement of the Project 3 1 Inputs 3 1 1 Japanese Side 1 Japanese researchers involved in project activities The research activities under the Project have been carried out in Panama and Japan The number of researchers that participated in the research activities in Japan is 36 in total in the Kinki University including undergraduate students Among them 18 researchers participated in the research activities both in Japan and Panama For further details refer to the Annex 3 2 Dispatch of Japanese experts researchers One long term expert project coordinator and 18 researchers have been dispatched to Panama in Short term in the fields of development of larviculture technologies early life history study
194. the same timing as the visits of the researchers of the Kinki University 4 Impact Premature to assess 1 Prospect on achieving the Overall Goal in future Resource management of two tuna species in Panamanian waters and jurisdiction area is strengthened Findings of research activities of the Project can be contributed to better understanding of population structure of YFT and PBT and identification of major factors affecting fluctuation of tuna resources These results are expected to provide a scientific basis for better management of resources 2 Other impact potential effects impact in future a Impact on science The research results of the Project through submission of academic papers and presentations at international domestic conferences will contribute to advance in science in the fields of tuna resource management and aquaculture b Contribution to future development and promotion of aquaculture in Panama It is generally said that the knowledge accumulated and techniques acquired by the Project are highly applicable to farming of other marine species It is expected that counterpart personnel of ARAP contributes to the future development and promotion of aquaculture in Panama xiii 5 Sustainability likely to be high 1 Policy aspect Achieving the objective of the Project will contribute to sustainable management of marine resources and the development of aquaculture in the medium
195. to long term These areas are considered as important issues by the Government of Panama Therefore policy sustainability of the Project will be secured 2 Organizational and technical aspect and IATTC have carried out the joint research since 1985 at the Achotines Laboratory and is responsible for the conservation and management of tuna and other marine resources in the eastern Pacific Ocean and has carried out researches on biology and stock assessment of tuna Therefore it is very likely that this joint research structure continues in the mid to long term For ensuring technical sustainability it is fundamental that the counterpart personnel who have acquired related knowledge and technologies continue their works at respective organization 3 Financial aspect The budget allocation plan for the Project was agreed among ARAP IATTC and JICA at the first Joint Coordinating Committee meeting It is important to adhere to the agreement for ensuring financial resource in a cooperative manner 3 Factors that promoted realization of effects 3 1 Regarding project plan None 3 2 Regarding implementation process None 4 Factors that impeded realization of effects 4 1 Regarding project plan None 4 2 Regarding implementation process 1 Turnover of counterpart personnel of ARAP At the beginning of the Project ARAP exerted considerable efforts to assign counterpart personnel to all the research fields
196. year from May to June and from October to November in general It seems that the dispatch of researchers of the Kinki University is appropriate in terms of number of persons expertize and research capacity At the beginning of the Project the dispatch of Japanese researchers was concentrated in a relatively short period of time and it was necessary to coordinate the arrangement of research materials and facilities in order to ensure smooth conduct of the research activities At present coordination of the timing of researchers visit to the Laboratory has been relatively improved and bence there is no serious difficulty observed over the use of the laboratory facilities The procured equipment by Japanese side is adequate for the research activities and has been used effectively In the case of ocean cage for juvenile fish rearing there is around one year delay in procurement and the related activities have not yet started There is a set of equipment that requires computer software for operation It was reported that the operating system of the computer is Japanese version windows 7 and one of the software is also Japanese version Therefore it is difficult to operate these system and software for the counterpart personnel of ARAP and IATTC In addition there are several pieces of equipment which instruction manuals are written in Japanese In order for proper use and maintenance of equipment instruction manuals in English or Spanish ate nece
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202. 3 Yang Su Kim AMaho Inomata Awa Z Tsukasa Sasaki Tomoki Honryo Yoshifumi Sawada Effect of rotifers enriched with taurine on growth and transformation of red sea bream larvae The 2012 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University September 2012 4 ATakayuki Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Effecis of temperature and food on the survival of cultured bluefin tuna larvae The 2012 autumn meeting of the Japanese Socicty of Fisheries Science National Fisheries University September 2012 5 Yoshifumi Sawada Tomoki Honryo Yasuo AKazuhiro Higuchi AMasato Awa OAmado Cano O Vemon Scholey ODaniel Margulies Teratogenic environmental factors to fish embryo low CO2 and high CO2 The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology March 2013 6 Toru Kobayashi Tomoki Honryo Yasuo Yoshifumi Sawada Il Adames OCano A O Scholey V OMarguries D Naoki Yanagishita Gonad formation process of cultured yellowfin tuna The 2013 spring of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology March 2013 7 Gentoku Nakase Tomoki Honryo Amado Cano ODa iel Margulies Yoshifumi Sawada Improvement of yellowfin larvae initial survival by Nanokuroropushisu prior addition The 2013 spring meeting o
203. 4 Impacto Dado que es prematuro describir el logro de la Meta Superior en el futuro y el impacto del Proyecto al momento de la Revisi n Intermedia s lo se describe el impacto provisional 4 4 1 Perspectiva para lograr la Meta Superior Meta Superior fortalece la gesti n de recursos de dos especies de at n en las aguas paname as y 18 121 el rea de jurisdicci n de la CIAT Medidas elaboradas de gesti n de recursos basadas en los resultados del Proyecto informaci n biol gica mejorada sobre las dos especies de at n Indicador Los resultados de las actividades de investigaci n del Proyecto pueden contribuir a una mejor comprensi n de la estructura de la poblaci n de YFT y e identificaci n de los principales factores que afectan a la fluctuaci n de los recursos atuneros Se espera que estos resultados puedan proporcionar una base cient fica para un mejor manejo de los recursos 4 4 2 Otros Impactos Bay varios efectos e impactos potenciales en el futuro como se describe a continuaci n 1 Impacto en la ciencia Los resultados de la investigaci n del Proyecto mediante la presentaci n de trabajos acad micos y presentaciones en conferencias internacionales y nacionales contribuir n al avance de la ciencia en el campo de acuicultura y manejo de los recursos de at n 2 Contribuci n al futuro desarrollo y promoci n de la acuicultura en Panam En general se dice que el conocimiento acumulado
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207. 9 11 OVernon Scholey ODaniel Margulies OJeanne Wexler and OMaria Stein Achotines Laboratory Captive culture of yellowfin tuna Thunnus albacares for research and investigation World Aquaculture Society World Aquaculture Magazine 12 Tomoki Honryo International research and tuna farming Wakayama version of the Sankei Sinbun un peri dico japon s 27 Julio 2013 153 Anexo 13 Lista de presentaciones en las conferencias internacionales y conferencias en Jap n 1 Presentaciones en los simposios y conferencias internacionales 1 Yoshifumi Sawsada Aquaculture technology for Pacific bluefin tuna life cycle completion Symposium of Taiwan and Japan Aquaculture Engineering on the Environment Balance Management and Technology Octubre 2010 National Taiwan University 2 Yoshifumi Sawada PRESENT STATUS OF TECHNOLOGY AND ITS PRACTICAL APPLICATION IN TUNA AQUACULTURE AND RESTOCKING IN JAPAN Aquaculture Europe 2011 Oct 18 21 Rhodes Grecce 3 Y Sawada Comparative Studies of the Reproductive Biology and Early Life History of Two Tuna Species for the Sustainable Use of these Resources SATREPS Simposio de Lanzamiento del Proyecto 17 de Nov de 2011 Ciudad de Panam 4 S Miyashita Representative Research Institute Kinki University SATREPS Simposio de Lanzamiento del Proyecto 17 de Nov de 2011 Ciudad de Panam 5 Kobayashi Giving the rescarch content outline of the Spawning Ecology Team SATREPS Simposio de
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210. E LEA M SSH UR E CO A LME 5 E ES W E ARREST S ELTE TRIAL LARA RUE NA C 4 HE AZ 18148 0 0 e He a Co WH KOLAC Y Lo we NO LWRZIA EORLERO HL HE suq QT CE MEH YOK Co HEN SESESETIZIA EB gt SCAM 2 X OBIE ZERO lt dj Co F12F 4 Bee HU Qe EESCAFSECO WR GHRIN use m LEY AP e o m SB E 9 5v TOC Sexe G SED MLS AD 438 LINZ SEU 48 B GEO SNA AUFL ME OEA EMELEC UE COS ALK RMS CMM EA ES VIA LT CELE LL 2 7 7 ou BE EL AU SOR CO WA L ESPERA RAS ZZ Z 2 G8 H Cog 322 ELO S Ego S er 3x EEES LENNON AME BE o d O BORD SEED AOL UG BE TO 2168 H SBM CORE Mote C 3 2 m 41 Z e LHR ROUGE O A OF 2 xig LN c BIR 2 SLA ES PAILS RAS NE AEG AU TEL OY YO 2 IE RUE 2 Q SIM O EBRIO DM ELL ROS C HARE SUYA 2 6 SLU SEC CUR MAA O RO 0 8 AI ER SEHD DBA eR d LEM SX 7 co 1k H 3 6 BO 36 OWES CO 3 2 ERA SD A
211. Exposiciones Gr ficas con Carteles en las Conferencias 1 AShinichiro Hashimoto Tomoki Honryo Yasunori Ishibashi Proper light wavelength for Bluefin Thunnus orientalis larvae The conference of the Zoological Society of Japan No 82 Septiembre 2013 en Asahikawa 2 Honryo T Yamamoto H Hirose H Kaze H Sawada Y The effect of EAS dietary fatty acids on survival Growth and lipoprotein lipase 2 and PPAR gene expression in juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 3 Kim Y S Aoki T Yamamoto S Ishimoto K K Murata O Sawada Y Effect of starvation on survival rate and body composition of juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 4 Yang Su Kim ODelgado D 5 OScholey Yoshifumi Sawada Studies on proper breeding water of yellowfin tuna larvae in embryonic development and early larvae stages The 2012 spring meeting of the Japanese Society of Fisheries Science National Fisheries University Marzo 2012 5 Tomoki Honryo OGuillen Olbarra J OMargulies D OScholey V Toru Kobayashi Yoshifumi Sawada Effect of water temperature on yellowfin tuna egg generation speed and hatching time The 2012 spring meeting of the Japanese Society of Tishcrics Soicncc National Fisheries University Marzo 2012 6 Yasunori Ishibashi A Yoshiki Tsutsumi Taro Matsumoto Tomoki Honryo Yoshifumi Sawada and Yasu
212. However 6 counterpart personnel were resigned from their posts Although vacant counterpart posts were promptly replaced by others but capacity building of these new counterparts needed to be re initiated 2 Communication among researchers involved in the Project It is reported that communication between Japanese researchers and their counterpart personnel has not been conducted smoothly in some occasions It was indicated that such poor communication might have affected efficient implementation of the project activities 3 Advance planning of research work at the Achotines Laboratory At the Achotines Laboratory it is required that research plan is submitted to the administrator of the Laboratory well in advance of researchers arrival in order to ensure coordinated use of laboratory facilities and equipment As for ARAP researchers duty trip arrangements need to be made since most of them need to travel to the Laboratory from their assigned places As such they need at least 30 days of lead time for application and preparation of their duty trips It is reported that there were a number of cases that sufficient time was not given before the conduct of joint research activities 5 Conclusion The Joint Mid term Review Team has confirmed that the project activities have shown a good progress in general As the results of the project activities scientific knowledge and information on early life history spawning ecology
213. Lanzamiento del Proyecto 17 de Nov de 2011 Ciudad de Panam 6 Giving the research content outline of the Nutrition and Feed Development Team SATREPS Simposio de Lanzamiento del Proyecto 17 de Nov de 2011 Ciudad de Panam 7 Y Ishibashi Giving the research content outline of the Early Growth and Survival and Rearing Technology Development Team SATREPS Simposio de Lanzamiento del Proyecto 17 de Nov de 2011 Ciudad de Panam 8 Biswas B K Biswas Idomioto N Kita Y K Dietary alternative protein sources for juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 2012 9 Y Sawada Y Agawa T Honryo Y S Kim Miyashita Tuna aquaculture and resource management Aquaculture America 2012 2nd March Las Vegas 10 OV Scholey OD Margulies Wexter Santiago Reproductive biology and spawning patterns of yellowfin tuna Thunnus albacares Aquaculture America 2012 2 de Marzo Las Vegas 11 OD Margulies OV Scholey OJ Wexler OM Santiago The use of indices of wind induced microturbulence and larval growth as indicators of pre recruit survival of yelloefin tuna Thunnus albacares Aquaculture America 2012 2 de Marzo Las Vegas 12 OJ Wexler OD Margulies Scholey Santiago Temperature and dissolved oxygen requirements for survival of yellowfin tuna Thunnus albacares larvae Aquaculture America 2012 2 de Marzo Las Ve
214. Masato Awa Tomoki Honryo Toru Kobayashi Michio Kurata Tokihiko Okada and Yoshifumi Sawada Identification of male and female characteristic DNA marker of aquacultured pacific bluefin tuna Thunnus orientalis Aisa Pa fic Aquaculture 2013 Ho Chi Minh December 2013 20 Tomoki Honryo Michio Kurata Ai Sumitomo and Yoshifumi Sawada The explore of possible initial swim bladder inflation periods and time of day on Pacific bluefin tuna Thunnus orientalis World Aquaculture Society 2013 Ho Chi Minh December 2013 21 Tomoki Honryo Angel Guillen Teruyoshi Tanaka O Amado Cano ODaniel Margulies O Vernon Scholey and Yoshifumi Sawada Effects of water surface condition on survival growth and swim bladder inflation ration on yellowfin tuna Thunnus albacres larvae Asia Pacific Aquaculture 2013 Ho Chi Minh December 2013 22 Yoshifumi Sawada AToshio Kaga Yasuo Agawa Tomoki Honryo Yang Su Kim Michio Kurata Yasuhiro Nakatani Toshio Tamura Tokihiko Okada and Shigeru Miyashita Growth analysis in artificially hatched Pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh December 2013 23 Maho Inomata Tomoki Honryo Yasuo Agawa Tokihiko Okada Toshio Tamura and Yoshifumi Sawada Research on handling technology improvement and stress response of juvenile Pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh December 2013 24 OMaria Stein ODaniel Margulies OJea
215. NA markers for sex determination in the early life history of YFT identified A sex linked DNA marker of tuna has been identified only with the full cycle cultured PBF at the Kinki University However an identified DNA marker can be used only for full cycle cultured PBF In order to identify the sex linked DNA marker of YFT it is necessary to examine neighbor sequence of the sex linked DNA marker of PBF and to identify the coding region and then to investigate the polymorphism of YFT Indicator 4 3 Parasitism of YET described Parasite examination on dead YFT broodstock from the Achotines laboratory has been conducted There was no parasitic infection which seriously affects the health of the YFT broodstock The description of parasitism of YFT related with this indicator has been completed therefore this component has been achieved Indicator 4 4 25 of improvement of 1996 2000 success rate from capture to healthy YFT broodstock attained In order to improve methods for capture and transportation of YFT body temperature changes in spine during transportation have been investigated It is suggested that stress of YFT continues for several hours after capture Methods for reducing stress of fish after transfer into fish tank will be examined It is also planned to propose new and improved methods regarding the transfer of fish into the fish tank The activities related with this indicator will be completed in the next year and it is expected t
216. O de MANI Ep BORAT KR g 8E Hel Sy 3 3 BS O 3 REESE OM rt ARO amp se e DEO AM o x BLASER SEAGATE CET gd xil V Y 2x BE Co BE 380 4 GAME IS 11 001 8 2 H B TAAR US AER O AT A ABE CHRE 29 O B iS Jm 095 508 Ae Ll EQU EA LY APE 3 G BAH RAN J 38k Q 2 eRe Z gt n RUS DRC MRA ZZ CL 298 1 HOR CRAM 6 2 HOLIY REA GAL CSE gt CO3SYSE T x LLAATG MOAT ALL UMS BEC AE MIWA DA HE SECAR REESE SE SESS OO SH A ee NUR BHE PI GOL ALVES uz COMBS UR 3 3 5 3 3 1 1 1 3
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220. S 0A1BJU9 T OXOUV 49NreZ Snf A0N 87 gureueq oder epelequis PUIEUE Y urened VOI E e PUN AoN LZ LI 9p eps WAN 00 71 GORY 00 60 UDISI El 501907911 SO 00 91 ope ses 00 21 SEIN sep worseneag SSULIOJU 2140 222 9p 00 61 sp U9IDEZITEUTI 00 01 91 UNT 20 52 ST pepnt pud pureuq op ap pepmo epis en Pumuta sp EPES pepnr ep ugioeiogetq SSUOIUNSY STAR opussnjour Sp S ULOJO ap oraexoqerq 00 21 PPH 0 gureueq OPeISPrT 00 80 4 0 sounou jap 00 61 ssuorospu tuooar SOL ap sanrzoJuT 20PRLOQ 00 11 Byun IOOO WISIN ep aoo euan 00 60 398900081 Soxqurorun 19309 UYI
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222. T JIPO 5913 1591 3154 1800691E Zsgiduu00 acuda 001 mmmoS WE uoder 911 A EE sa os CC se eer o espyp w er j10puadda pph 0569 19 2 api PT spaNG vodef 881 Pl 6 Ly6 Spor pue pnibi 30 2058 di B y ZD5 pue jnauamseou pimbi 20 93QsETd Py 2 J1opuodds noz oz 32888 used oot 91196 Snipuet pue 1u ur msu ur pinby 20 2454 02 m 001 01 3200 fest 61476 Suypueu pinbr 20 pij 92 j20pusddo I 07 7 pdd ONSET wer per El Lp6 Surpust pue juomamsesm Joy way onse q veder popuaddo pw 9 11 20 redid 95544 wer jer popueddo py 02 FIopradda 2 19 did nsed wer jm YIA 95304 PEZ pc s tov ope 10103 AWOL asy oyayi 151 ODE PAL 10109 ur LOEKIE OA mn 05 Pb VIO weder 641 ones ser lail 10668 2 saad oot NO SV Sumeos mnN ugder 1691 0950 108N ON sur ss lt sotq SSON UREA 96 pondo dmyo wer lil
223. a de Implementaci n del Proyecto y Proceso de Implementaci n del Proyecto 4 Resultados de la Revisi n 4 1 Relevancia 4 2 Efectividad 4 3 Eficiencia 4 4 Impacto 4 5 Sostenibilidad 4 6 Conclusiones 5 Recomendaciones Anexos Anexo 1 Programa de la Revisi n Anexo 2 Matriz de Dise o del Proyecto versi n 0 1 Anexo 3 Lista de Investigadores Japoneses Involucrados en las Actividades del Proyecto Anexo 4 Env o de Expertos y Investigadores Japoneses Anexo 5 Personal Contraparte Capacitado en Jap n Anexo 6 Equipo Suministrado por la Parte Japonesa para el Laboratorio Achotines Anexo 7 Equipo Adquirido para el Instituto de Investigaci n en Jap n Universidad de Kinki Anexo 8 Costo Local Asumido por la Parte Japonesa Anexo 9 Lista de Personal Contraparte Involucrado en las Actividades del Proyecto Anexo 10 Equipo Adquirido por la Instituci n Contraparte Anexo 11 Costo Local Asumido por las Instituciones Contrapartes Anexo 12 Lista de Publicaciones 13 Lista de Presentaciones en las conferencias internacionales y conferencias en Jap n 102 ARAP 5 Abreviaturas y Acr nimos Autoridad de Recursos Acu ticos de Panam CIAT Comisi n Interamericana del At n Tropical Ministerio de Econom a y Finanzas At n de Aleta Azul del Pac fico Matriz de Dise o del Proyecto Plan de Operaci n Comit Conjunto de Coordinaci n Registro de Discusiones A
224. a de PBF para examinar la tolerancia al hambre en el per odo larval y la densidad de la presa adecuada etc El an lisis de los datos est en marcha En el caso de YFT CIAT ha briadado resultados de la investigaci n Por lo tanto las similitudes y diferencias entre YFT y PBF ser n identificadas despu s de obtener los resultados del an lisis 3 Valor nutricional de alimentos naturales y artificiales Se llev a cabo la investigaci n de la composici n general de cidos grasos de rot feros que fueron fortificados artificialmente y de cop podos como material de alimentaci n la fase inicial de crecimiento El contenido de l pidos se increment de manera significativa por el uso de rot feros que fueron enriquecidos artificialmente con DHA Protein Serco producida en B lgica Por otra parte no hubo diferencias entre el contenido de prote na y ceniza En cuanto a una composici n general de cop podos que se recogi cerca Laboratorio Achotines el contenido de prote nas y l pidos fueron menos que los contenidos de prote nas y l pidos de PBF y el contenido de cenizas fue m s que el contenido de cenizas de los rotiferos fortificados Como resultado se encontr que la fortificaci n de rotiferos es eficaz para YFT En resumen si reanuda el desove de reproductores se completar la identificaci n de las similitudes y diferencias en relaci n con este indicador en el per odo del proyecto 3 3 4 Resultado 4 Se desar
225. aparte sobre su plan de capacitaci n se requiere para mejorar la efectividad de tal capacitaci n Tambi n se recomienda la asignaci n de un supervisor para cada participante en la capacitaci n quien es responsable de garantizar la calidad global de la capacitaci n Temas relacionados con la Administraci n del Proyecto 6 La asignaci n oficial de un Oficial de Enlance En este proyecto el apoyo administrativo brindado por la sede central de la ARAP es esencial para la implementaci n normal de Proyecto fin de garantizar la continuaci n de esta funci n es recomendable que la asigne un Oficial de Enlance como punto focal t cnico para el Proyecto quien ser nicamente responsable entre otras cosas de la comunicaci n con el Proyecto recepci n y seguimiento de la tramitaci n de los documentos y solicitudes y encargarse de otros asuntos administrativos del Proyecto 7 Continuaci n del esfuerzo para garantizar la asignaci n de las contrapartes de la ARAP A pesar de las dificultades de log stica los contrapartes de la ARAP tienen que viajar a Achotines desde sus reas asignadas de trabajo las contrapartes de la ARAP han estado jugando un papel importante en la realizaci n de diversas actividades de investigaci n Los esfuerzos de la ARAP para seguir proporcionando 21 124 estos insumos cr ticos son indispensables por lo tanto se deber a asegurar que las contrapartes de la ARAP existentes contin en p
226. articipando en el Proyecto durante el per odo restante del Proyecto 8 Publicidad del Proyecto La presencia y relevancia del Proyecto requieren ser ampliamente difundidas al p blico en general en Panam y Jap n a fin dc facilitar su comprensi n as como lograr el apoyo a las actividades dcl mismo Con este prop sito se recomienda que el proyecto prepare y difunda materiales informativos tales como folletos y boletines informativos que promuevan el entendimiento del marco del Proyecto objetivo resultados esperados y principales actividades Considerando el hecho de que los recursos atuneros son recursos importantes compartidos en el Oc ano Pac fico que requieren esfuerzos regionales para su manejo se puede decir que los hallazgos cient ficos del Proyecto son tambi n colecciones de informaci n valiosas desde el punto de vista regional En vista de lo anterior es recomendable que todas las partes concernientes del Proyecto consideren organizar un seminario internacional o regional para la difusi n de los resultados del proyecto a un p blico m s amplio 9 Contribuci n Financiera A fin de garantizar la estabilidad financiera del Proyecto se insta a la ARAP CIAT y JICA se adhieran a los acuerdos concertados en las reuniones del CCC 10 Mejora en la gesti n y seguridad de los equipos En este Proyecto se ha suministrado una variedad de equipos e instrumentos t cnicos los cuales son utilizados por m ltiples usuarios Reciente
227. as correcciones t cnicas hechas a la PDM seg n se describe en la siguiente tabla Items Original Revisi n realizada Motivos Indicador para el S ntesis de la informaci n S ntesis de la informaci n La informaci n que ser Prop sito del Proyecto biol gica aplicable al manejo cient fica y t cnica aplicable al sintetizada no es del recurso de especies atuneras manejo del recurso de especies solamente biol gica el cual se difunde medio de atuneras el cual se difunde por publicaciones medio de p gina web publicaciones seminarios talleres regionales p gina web seminarios o talleres regionales Inclusi n de un medio de verificaci n adicional Medios de Verificaci n para el Prop sito del Proyecto Publicaciones del Proyecto P gina web oficial del Laboratorio de Achotines Procedimientos de seminorios talleres Publicaciones del Proyecto 9 P gina web oficial del Laboratorio Achotines Compendio de ponencias de seminarios y talleres Informe sintetizado Producci n de juveniles de de 20 cm despu s de un perfodo de cr a extendido Producci n de al menos 25 Correcci n T cnica juveniles de YFT de 20 em despu s de 3 meses en la jaula marina Indicador el Resultado 4 para 4 Asignaci n de Coordinadores de Investigaci n Bas ndose en l
228. as diferentes temporadas de desove 12 Investigaci n de factores ambientales que afectan el desove de YFT 1 3 Investigaci n de los efectos del estado nutricional en el desove de 1 4 Desarrollo de un m todo sencillo y completo para examinar el estado fisiol gico de peces reproductores larvas y juveniles de YFT y PBF Biblioteca de ADN complementario y desarrollo de rganos viscerales incluyendo las g nadas 2 1 Examen de D loop mitocondrial como m todo para identificar la l nea materna de YFT 2 2 Verificaci n del m todo a trav s del an lisis de muestras de YFT silvestres capturados seleccionados para examinar sus l neas maternas 3 1 Investigaci n comparativa del desarrollo temprano los efectos de los factores fisicos y qu micos en YFT y PBF 3 2 Investigaciones comparativas de las caracter sticas de visi n y la reacci n de larvas de YFT y PBF a la luz 3 3 Investigaciones comparativas de la ecolog a de alimentaci n comportamiento crecimiento y supervivencia de YFT y PBF en las etapas tempranas de vida 3 4 Investigaciones comparativas del valor nutricional de tipos de alimentos artificiales y naturales de YFT y PBF 4 1 Desarrollo de herramientas para el an lisis gen tico y manejo de 4 2 Recolecci n de informaci n sobre el mancjo de salud de YFT 4 3 Mejoramiento de los m todos de captura y transporte de candidatos para ia poblaci n de reproductores de YFT 4 4 Desarrollo de tecnolog as de criadero y de
229. as recomendaciones hechas por el Equipo la ARAP la CIAT y la Universidad de Kinki designaron a los Coordinadores de Investigaci n que se indican a continuaci n ARAP Ing Amado Cano M Sc Yazmin Villarreal M Sc Thelma Quintero CIAT Ing Vernon Scholey Dr Daniel Margulies Universidad de Kinki Dr Yasuo Dr Amal Kumar Biswas El CCC aprob la designaci n de los Coordinadores de Investigaci n que se se alaron anteriormente 5 Contramedidas frente a la paralizaci n del desove del at n de aleta amarilla El CCC debati sobre las contramedidas para hacer frente a la actual paralizaci n del desove del at n de aleta amarilla y acord iniciar inmediatamente los preparativos para la posible inyecci n de hormonas como una alternativa pr ctica para inducir el desove en un peque o grupo de reproductores en un tanque de reserva Se seguir explorando otras alternativas 6 Otros asunto 1 A fin de garantizar la estabilidad financiera del Proyecto se reiter que la ARAP CIAT y JICA deber n cumplir con los acuerdos concertados en las reuniones del CCC 2 La CIAT solicit a la JST y a la JICA que exploraran la posibilidad de proporcionar asistencia financiera para los viajes al Jap n del personal internacional de la CIAT para participar en los trabajos de investigaci n en el Laboratorio de Pesquer a de la Universidad de Kinki y asistir peri dicamente a una importante reuni n cient fica internacional Esta so
230. ated to the Project including project reports questionnaires replied by Japanese researcher expert and counterpart personnel in Panama and prepared an evaluation grid which listed the specific review points and the data collection methods 1 4 2 Review activities in the Republic of Panama The Joint Mid term Review Team visited ARAP and the Achotines Laboratory and carried out a series of interviews and discussions with counterpart personnel in Panama and Japanese researchers expert etc 1 5 Methodology of the Mid term Review 1 5 1 Method of Review The Project was reviewed jointly by the Team based on materials showing the framework of the Project such as PDM PO and the Record of Discussion R D The review work consists of analysis of project reports operation logbooks of the equipment field surveys and interviews with the researchers of ARAP and the Achotines Laboratory and Japanese researchers expert Furthermore a thorough examination of all the relevant information obtained was conducted by applying the following five evaluation criteria 1 5 2 Evaluation Criteria Five Evaluation Criteria 1 Relevance Relevance refers to the validity of the Project Purpose and the Overall Goal in connection with the development policy of the authorities concerned of Panama as well as the needs of the beneficiaries and the assistance policy of Japan 2 Effectiveness Effectiveness refers to the extent to which the expected benefits of the Projec
231. biological resources This Project is within the scope of JICA s focal area in Panama The Kinki University of Japan succeeded to close the life cycle of PBF for the first time in the world and has accumulated considerable knowledge and technical expertise over more than 40 years of research work Partnership with the Kinki University is a great advantage in terms of conducting comparative studies on and in an efficient way In the Achotines Laboratory there are YFT broodstock culture tanks where spawning of YFT occurs almost all the year around As compared to the similar facilities in other areas where spawning may only be observed once a year for the duration of several weeks the Achotines Laboratory offers an optimal environment for conducting reproductive biology and early life history studies of YFT 2 Effectiveness High The project activities have shown a good progress in general and reasonable pieces of research outputs have been produced as indicated by a number of research papers published or prepared so far However there is a concern that prolonged halt in spawning of YFT at the Achotines Laboratory may seriously affect the timely conduct of planned research activities which would consequently affect negatively the level of achievement of the Project Purpose 3 Efficiency Moderately High 1 Inputs by Japanese side It seems that the dispatch of researchers of the Kinki University is appropriate in terms of numbe
232. boratory Japanese Implementing Organization Kinki University 1 Background of the Project Tuna fishery is an important industry for the Republic of Panama hereinafter referred to as which generates valuable foreign exchange earnings for the country The production from the fishery is processed and exported to the North American and European countries In recent years tuna resources show a decreasing trend and the excessive fishing pressure is said to be a major cause of the problem Tuna resources are highly migratory species distributing widely in the Pacific Ocean and because of this nature these resources are exploited by a large number of coastal countries of the Ocean Yellowfin tuna hereinafter referred to as Y FT and Pacific bluefin tuna hereinafter referred to as PBF are major target species of the tuna fishing industry and Panama is one of the major producer countries of YFT The volume of tuna and tuna like species catch of Panama is the third largest among Latin American countries Panama is also an important country where foreign fishing boats land and transfer their catch of tuna In terms of scientific research on tuna resources the Panama is the country where the Inter American Tropical Tuna Commission decided to establish its research facility the Achotines Laboratory It is the only research station of regional fisheries bodies where YFT broodstocks ar
233. by the Project In the Achotines Laboratory there are broodstock culture tanks where spawning of YFT occurs almost all the year around As compared to the similar facilities in other areas where spawning may only be observed once a year for the duration of several weeks the laboratory strategically located in Achotines offers an optimal environment for conducting reproductive biology and early life history studies of YFT 4 2 Effectiveness The Project Purpose is expected to be produced in an effective way by the end of the Project The project activities have shown a good progress in general and reasonable pieces of research outputs have been produced as indicated by a number of research papers published or prepared so far However there is a concern that prolonged halt in spawning of YFT at the Achotines Laboratory may seriously affect the timely conduct of planned research activities which would consequently affect negatively the level of achievement of the Project Purpose Tt is envisaged that seasonal rise of sea temperature that usually occurs in April may trigger the resume of spawning activities of YFT and the Project needs to closely monitor the environmental factors of the broodstock culture tank 4 3 Efficiency The efficiency of the Project is considered to be moderately high based on the facts described below 1 Inputs by Japanese side The researchers of the Kinki University have visited the Achotines Laboratory twice a
234. c changes in tolerance to high and low temperature stressors of larval and juvenile Pacific bluefin tuna Thunnus orientalis Aisa Palfic Aquaculture 2013 Ho Chi Minh Diciembre 2013 158 LAA Spanje astoortpood vj vj XU us sua op L APEL IP SYJON DA suodin manda cay sp aaa souspred L vzed lt JENS cedo op obeu aaar uppen x 18 2 ohm osnpusS po SAULEI COMA za A serosjttro sod sonum LIA sp ucfoce 3nb upizonpuid spousal aL SS 2018 Z JHd lt LAR see op ERA RIE FE IP SA FIRS 304 ALLER soormmnb A 200427 2261507 40 20132301 OEP PLY gmt l g SENER Sas Jour cred SOPAS sopemidto SANTS LIA 9p FENSONIN 53523 d OPONA 7 2 sony q 0 05 1 2 KT SPT TRS Sp US SP TUE ypAtsarpuoha Jod bonu op 24 1 m s ROUDUTY Sn id seu m Aaymadas OS vto br 5a m gor Jude wogonpald Suy y
235. cad micas 1 Yoshiki Tsutsumi Taro Matsumoto Tomoki Yasuo Agawa Yoshifumi Sawada and Yasunori Ishibashi Effects of light wavelength on growth and survival rate in juvenile Pacific bluefin tuna Thunnus orientalis Environmental Biology of Fishes en prensa 2 Yoshifumi Sawada AToshio Yasuo Agawa Tomoki Yang Su Kim Masahiro Nakatani Okada O Amado Cano O Daniel Margulies and Vernon Scholey Growth Analysis in Artificially Hatched Pacific Bluefin Tuna Thunnus orientatis Aquaculture Science en prensa 3 Sakamoto Wataru ATakayuki Ohnishi Yuichi Tsuda Effect of food and temperature on swimming behavior of juvenile bluefin tuna Proceedings of 19th Intemational Symposium on Biotelemetry Peer reviewed proceedings en prensa 4 OMaria Stein ODaniel Margulies OJeanne Wexler O Vernon Scholey Yang Su Kim Tomoki Honryo ATsukasa Sasaki and Yoshifumi Sawada Comparative studies of enrichment media for producing nutritionally enhanced prey and their effects on growth survival and cohort biomass of Pacific bluefin larvae Presentado a Marine Ecology Progress Series 5 O Angel Guillen Tomoki Honryo OJuan Ibarra OAmado Cano ODaniel Margulies O Vrnon Scholey OJeanne Wexler OMaria Stein Toru Kobayashi and Yoshifumi Sawada Effect of embryonic development of Yellofin tuna Thunnus albacares Presentado a Aquaculture Science 6 Tomoki Honryo Naoki Kaze Hiroshi Yamamoto Hideki Hi
236. ce at the larval period and the appropriate prey density etc Analysis of the data is underway In the case of YET LATTC has provided research results Therefore similarities and differences between YFT and PBF will be identified after obtaining results of analysis 3 Nutritional value of natural and artificial feed Investigation of the general composition and fatty acid composition of artificially fortified rotifer and copepods was conducted as feeding material at the early growth stage The lipid content was increased significantly by using rotifers that were artificially fortified with DHA Protein Serco produced in Belgium On the other hand there were no differences between the contents of protein and ash As for a general composition of copepods which were collected near the Achotines Laboratory the contents of protein and lipid were less than the protein and lipid contents of PBF and the content of ash was more than the ash content of the fortified rotifer As a result it was found that the fortification of rotifer is effective for YFT lt 3 3 4 Output 4 Fingering production technologies that support early life history study of YFT are developed Indicator 4 1 clone for the brood stock developed As for PBF BAC using DNA extracted from testis was developed As for YFT BAC using DNA extracted from liver was developed BAC clone development related with this indicator has been achieved Indicator 4 2 Sex linked D
237. citado en Jap n Trece 13 miembros del personal contraparte han participado en las capacitaciones en Jap n Los temas de la capacitaci n fueron desarrollo temprano y supervivencia de atunes supervivencia de atunes en las etapas de larva y juvenil enfermedades de peces y biolog a molecular Para m s detalles v ase el 5 4 Suministro de Equipo 1 Equipo suministrado por la Parte Japonesa para el Laboratorio Achotines JICA ha suministrado equipos para actividades de investigaci n y cultivo de peces cuyo monto total es de aproximadamente novecientos veintisiete mil Balboas 927 000 00 La lista detallada de los equipos aparece en el Anexo 6 2 Equipo suministrado al instituto de investigaci n en Jap n Equipos para las actividades de investigaci n cuyo monto total es de aproximadamente 24 8 millones de Yenes La lista detallada de los equipos aparece en el Anexo 7 5 Costos Locales Asumidos por la Parte Japonesa Los costos locales en Panam cubiertos por la Parte Japonesa para la implementaci n de las actividades del Proyecto es de aproximadamente doscientos sesenta y dos mil Balboas B 262 000 00 de D lares Norteamericanos en total hasta septiembre de 2013 Esto incluye los gastos para la ampliaci n del edificio principal con espacio adicional de oficina sala de reuniones y almacenamiento de equipos mantenimiento de equipos adquisici n de equipos compra de materiales consumible
238. culture development 2012 describes the importance of the development of aquaculture in harmony with environmental management and also specifically promotes research on YFT because of its high potential It can be said that the objective of the Project is in line with above mentioned Panama s policy objectives 3 Conformity to the assistance policy of Japan to Panama One of the important sectors for JICA s assistance work in Panama is environmental conservation which naturally includes conservation of the natural environment and biological resources This Project is expected to contribute to the sfrengthening of resource management of tuna species which is within the scope of JICA s focal area in Panama 4 Comparative advantage of technical cooperation by Japan The Kinki University of Japan is a leading university in the field of aquaculture related study which has succeeded to close the life cycle of PBF for the first time in the world Considerable knowledge and technical expertise on the culture of PBF have been accumulated in the university over more than 40 years of researeh work It can be considered that partnership with the Kinki University is a great advantage in terms of conducting comparative studies on YFT and BFT in an efficient way 1 Western Central Pacific Fisheries Commission 2 International Scientific Committee for Tuna and Tuna like Species in the North Pacific Ocean 16 e 5 Appropriateness of approach taken
239. d in juvenile Pacific bluefin tuna Thunnus orientalis Submitted to Fisheries Science 18 Mark Polinski Sho Shirakashi Andrew Bridle Barbara Nowak Transcriptional immune response of cage cultured Pacific bluefin tuna during infection two Cardicola blood fluke species Submitted to Fish and Shellfish Immunology 19 Gentoku Nakase Tomoki Honryo OLiliana Guerra ODiana Perz O Amado Cano O Daniel Margulies O Vernon P Scholey and Yoshifumi Sawada Addition of Nannochloropsis oculata in pre rearing water improves survivals of Yellowfin tuna Thunnus albacares larvae Submitted to Aquaculture Scicnce 2 Other publications 1 Yoshifumi Sawada An expertize concerning on the artificial hatching and larval rearing of the Pacific bluefin tuna Proceedings of the Symposium of Taiwan and Japan Aquaculture Engineering on the Environment Balance Management and Technology 312 336 2010 2 Kenji Takii Yesterday today tomorrow of bluefin tuna nutrition and feed research perspectives to inexpensive formula feed for larvae Aquanet April 2011 68 71 lt Po 3 Yoshifumi Sawada Toward the stable development of bluefin tuna artificial seedling production the branding of the product intellectual property of technology Suisankai March 2013 1538 14 15 4 O Daniel Margulies O Vernon P Scholey O Jeanne Wexler O Mar a S Stein Achotines Laboratory Home To Continuing Studies Of Tuna Early Life History the Global Magazi
240. daily basis It is recommended ARAP take a lead role in addressing this issue END lt 1 301394 00 41 61 91 SUISOTD 00 91 I sxaqoeaso Diury pue q 10893 Cr PHIN 0041 HD uon 06 21 OLLYLJO d O 01 Ma1413 u 00 11 Jo weudmbo pue SSEA 06 60 OLLVIJO Peor 10 452100 00 60 qeTILLVI 1 00 60 moy woy amuedodq 00 80 apis osauede ws 00 61 fsepad sounouoy BALI 00 61 somoqoy 1 00 81 UOISSIJAT YIM Zones 00 61 TONEMEAT Un 00 41 0 0011 sorgo eureueg WIM FUNN 00 60 Buea 9129 uotssrAr 00 1 SONT per Suodew Sayed ME isepaq Pue g 00 61 LIVI ui mp 01 SUIAOJN ESA 4 2 06 61 sepad ayay 4 O Wm MAAJA 02 01 AON CI VAN 05 6 2 mapag 06 8 4 9 00 91 epis vureueq Sunooy 3302490 00 61 ML AONZ Z Qi zo Zs pwoO 06 01 20190 P
241. diciones ambientales clima temperatura temperatura del agua de cr a etc de YFT se han registrado todos los d as en situaci n de cautiverio en el Laboratorio Achotines El an lisis de los datos recogidos se llevar a cabo en la etapa posterior En ese momento se encontr que el rango de temperaturas del desove de YFT bajo la condici n de cautiverio fue de m s de 24 Se suspendi el desove de YFT en el de 2012 cuando se produjo una marea roja en el agua del mar cerca del Laboratorio Achotines a ra z de las fuertes lluvias en la estaci n lluviosa A partir de este evento se hace evidente que los cambios en la calidad del agua y la salinidad afectar an el desove de Adem s se encontr que la intensidad de luz y la fotoperiodicidad duraci n del d a no son los factores que afectan el desove En cuanto a la relaci n entre el desove y la fase de la luna se requiere recopilar datos adicionales Zp 113 En resumen se han identificado los efectos de tres factores ambientales rango de temperatura fotoperiado e intensidad de luz y se llevar a cabo el estudio de factores ambientales adicionales Tndicador 1 3 M todo bioqu mico desarrollado para determinar la calidad de huevos de YFT y PBF En cuanto a PBE la recolecci n de muestras de tejido visceral se llev a cabo para clonar la situaci n fisiol gica relacionada con los genes en el rgano interno En cuanto a YFT las
242. dores al Laboratorio se ha mejorado relativamente y por lo tanto se observa dificultad seria en el uso de las instalaciones del laboratorio Los equipos adquiridos por fa parte japonesa som adecuados para las actividades de investigaci n y se han usado efectivamente Roferente a las jaulas marinas para la cr a de peces juyeniles hay un atraso de acerca de un a o en su adquisici n y las actividades relacionadas no han comenzada todav a Hay un conjunto de equipos que requiere un programa inform tico para su operaci n Se hab a informado que el sistema operativo de la computadora utiliza la versi n japonesa windows 7 y uno de los programas tambi n utiliza la versi n japonesa Por lo tanto se hace dificil al personal contraparte de y CIAT operar dicho sistema y programa Adem s algunos de los equipos tienen manuales de instrucci n cscritos en japon s Para el uso y mantenimiento apropiado del equipo se requieren manuales de instrucci n en ingl s o espa ol Referente a las capacitaciones en Jap n en la mayor a de los casos las capacitaciones fueron efectivas en t rminos del fortalecimiento de la capacidad investigativa del personal contraparte en total participaron 13 personas Hubo casos donde la explicaci n de las objetivos de la capacitaci n y los m todos de investigaci n no se hicieron apropiadamente y las instrucciones al personal contraparte tampoco se hicieron adecuadamente 2 Insumos por la ARAP Se
243. e Scientific knowledge and information on the reproductive biology and early life history of two tuna species which are fundamental for the sustainable use of these resources are adequately obtained and synthesized Synthesis of biological information applicable to resource management of tuna species which is disseminated by means of publications website regional seminars workshops Indicator 1 It is expected that the research results will be compiled in a synthesized form and disseminated by means of three media publications website and regional seminars workshops It is also expected that the research results will be presented at regional and international seminars which will bc held before the end of the project term In addition biological information will be compiled in a book of which publication can be after the tennination of the Project 3 5 Project Implementation Structure and Process 3 5 1 Project Implementation Structure The project activities have been conducted by the researchers of ARAP IATTC and the Kinki University The following figure shows the conceptual implementation structure of the Project ARAP hendquinttnt Projest Director 1 Poket v font Research ndtochnical transferin Panama Counterpart otgalzal or ABAP JATIC Project Co Manager and 2 rz archers in USA 3 rescarchent at ho cholinos oy Shori term expe
244. e Panamanian authorities and IATTC 1 3 1 Japanese Mid term Review Team y Senior Advisor Japan International Cooperation Agenc Leader Mr Shunji Sugiyama PASE P vip JICA Cooperation Field Crop Based Farming Area Division Rural Mr Suguru Kubo Planning Development Department JICA or Graduate School o kture Divi 3 Evaluation IST Dr Koknbun Graduate School of Division of Biological Resource Sciences Tohoku University gt Chief Research Partnership for Sustainable Development Evaluation JST Ms Chihiro Inoue e 4 07 Ms Japan Science and Technology Agency Evaluation and 5 5 Mr Isao Dojun Consultant Chuo Kaihatsu Corporation Analysis 1 3 2 Mid term Review Team of the pm Organizations SEES ME ae Director of Aquatic Resources Authority of Lead Mr Edison Panama in Los Santos Tech Ministry of Eco 2 member Mi isa Rem nical Cooperation Coordinator Ministry of Economy and Finance MEE Representati Inter A meri Tropical T 3 Member Mr Osvaldo Alexis Silva pr D Das AME P Commission in Panama 1 4 Schedule of the Mid term Review The Mid term Review Study was conducted from November 11 to November 26 of 2013 as shown in the 1 1 4 1 Initial Examination in Japan The Japanese Mid term Review Team reviewed available documents rel
245. e kept and spawning is observed in captivity At the Achotines Laboratory various research activities are conducted in close collaboration with the Aquatic Resources Authority of Panama hereinafter referred to as ARAP Based on the background described above the government of Panama made a request to the Government of Japan to carryout scientific technical cooperation on Comparative studies of the reproductive biology and early life history of two tuna species Yellowfin and Pacific Bluefin tuna to ensure the sustainable use of these resources herein after referred to as the Project The Project was commenced in April 2011 as a 5 year project 2 Project Overview 1 Overall Goal Resource management of two tuna species Panamanian waters and IATTC jurisdiction area is strengthened 2 Project Purpose Scientific knowledge and information on the reproductive biology and early life history of two tuna species which are fundamental for the sustainable use of these resources are adequately obtained and synthesized 3 Outputs 1 Spawning characteristics of YFT and PBF are determined 2 The method to identify maternal line of YFT is established by using mitochondria D loop for analysis 3 Critical factors that affect survival of YFT and PBF in their early life history are identified 4 Fingering production technologies that support early life history study of YFT are developed 4 Inputs Japanese side
246. e ocho mil quinientos treinta y seis Baiboas B 8 536 00 La lista detallada de los equipos aparece en el Anexo 10 3 Costos de Operaci n del Proyecto Asumidos por las Instituciones Contrapartes La ARAP y la CIAT cubrieron gastos para rubros tales como combustible electricidad equipos y art culos consumibles para las actividades del Proyecto El monto anual asignado por cada organizaci n aparece en el siguiente cuadro Los costos detallados se presentan en ej Anexo 11 Unidad Balboas 22044000 n d Datos no disponibles todav a 4 Instalaciones suministradas por las instituciones contrapartes Las instituciones contrapartes ofrecen las siguientes instalaciones para las actividades del proyecto en el Laboratorio Achotines 1 Espacio de oficina para los expertos investigadores japoneses 2 Instalaciones y equipos de investigaci n del Laboratorio Achotines 3 2 Avance de las Actividades Programadas Las actividades del Proyeeto se han llevado cabo de acuerdo eon la y el PO desde el inicio del Proyecto Las actividades del Proyecto realizadas entre abril de 2011 a octubre de 2013 se encuentran la Tabla descrita a continuaci n con el progreso aproximado de las respectivas actividades planificadas en el momento de la Revisi n Intermedia bas ndosc en la informaci n proporcionada por los investigadores involucrados en el Proyecto 109 Sea Svp op GISOTOTTOtT vj sspe 19105509
247. e por el medio de publicaciones p gina web 14 117 seminarios o talleres regionales Se espera que los resultados de la investigaci n sean compilados en una forma sintetizada y difundidos a trav s de tres medios de comunicaci n publicaciones p gina web y seminarios o talleres regionales Tambi n se espera que los resultados de la investigaci n sean presentados en seminarios regionales e internacionales que se celebrar n antes de que finalice el plazo del Proyecto Adem s la informaci n biol gica ser compilada en un libro cuya publicaci n puede ser posterior a la terminaci n del Proyecto 3 5 Estructura y Proceso de Implementaci n del Proyecto 3 5 1 Estructura de Implementaci n del Proyecto Las actividades del proyecto se han llevado a cabo por los investigadores de la ARAP la CIAT y la Universidad de Kinki La siguiente figura muestra la estructura conceptual de implementaci n del proyecto SedeCenra dela Director del Proyecta Co Gerente dal Proyecto Capacitaci n en Jap n Investigaci n Conjunta y Transferencia T cnica en Panam Jostituciones It investigadores A Expertos de corto pleo AsesorJefe 4 JICA Ecolog a de desove gr Requerimientos de nutrici n gt Estudio del ciclo vital temprano MOU CIAT t Desart llo de laniovitum i t Otros 18
248. e tres diferentes organizaciones contrapartes est involucrado en la ejecuci n de investigaciones conjuntas y muchos investigadores vienen al Eaboratorio Achotines por corto plazo Tal naturaleza del trabajo de investigaci n inevitablemente requiere la comunicaci n por correo electr nico debido a que las reuniones en persona no siempre son posibles especialmente en la etapa de planificaci n previa a 15 visitas Sin embargo la eficiencia de comunicaci n depende en gran medida de la destreza y habilidad de los investigadores individuales y adem s existe la barrera de idiomas Como consecuencia se reportaron varios casos donde la comunicaci n se hizo problem tica Por lo tanto se exhorta que todas las personas involucradas en el Proyecto se esfuercen para mejorar la comunicaci n dentro del Proyecto se sugieren m todos pr cticos para atender a este problema cn las secciones siguientes 2 Asignaci n oficial de coordinadores de investigaci n Con respecto a la comunicaci n a nivel organizativo la definici n clara y expl cita de un canal de comunicaci n frecuentemente mejora la situaci n Tal puede ser establecido f cilmente a trav s de la designaci n de un punto focal o de enlace en cada organizaci n contraparte y en este proyecto este punto de enlace puede llamarse Coordinador a de Investigaci n quien actuar como el canal central para la comunicaci n entre las organizaciones Los Coordinadores de Investigac
249. ed to PBF It seems that the inputs made by are appropriate The issue of coordination of the research activities mentioned above was also relevant to LATTC researchers because their visits to the Laboratory were made at the same timing as the visits of the researchers of the Kinki University 4 4 Impact As it is premature to describe the level of achievement of the Overall Goal at the point of the Mid term Review only provisional impacts are described 4 4 1 Prospect for Achieving the Overall Goal Overall Goal Resource management of two tuna species in Panamanian waters and IATTC jurisdiction area is strengthened Indicator Resource management measures elaborated based on the project outputs improved biological information on two tuna species Findings of research activities of the Project can be contributed to better understanding of population structure of YFT and PBF and identification of major factors affecting fluctuation of tuna resources These results are expected to provide a scientific basis for better management of resources 4 4 2 Other Impacts There are several potential effects impact in future as follows 1 Impact on science The research results of the Project through submission of academic papers and presentations at international domestic conferences will contribute to advance in science in the flelds of tuna resource management and aquaculture 2 Contribution to future development and pro
250. eds jo uosieduoy 9035po0 q ur Surameds jo 3161053 1 1 1 oum Sujuweds JA JO von snsoAu 1 1 Surumedg eq 0 SHAY S9nfAnov pottueid poj2odxq ULAL PIN PUE SU ur oui Jo 5559014 TI9LIGISHICTSS 154202 rymy Soane jo RTE sentamos JO pajerduoo 214016 Sy pue pos jrutiog qroudo oAsp pue pam ysg 3uisn juzumiodxq pea Jo Alguenb pue pos go sad 30 0 Aq LJA Jo p ods Surunntas vogeogmuenQ PUE JAA jo sspuoAnf pue ut 4 jens jo SIS EUe pue sampans jo sm gue jo uongmuguo 319 uoretper A 1 01 2006151591 8 TeISdm IEM 520122 JO STOWE 5 JO JUODITSHQRISS pue uonejosp p Add LAK Jo 2128 1 04 YO HO JO siskfeur 9 1 Jgd THX JO ood 3095 THd pue LAA JO 2 1 2095 OVA LAK FO momdofexe sadi 3ueurusdxo z y c POO emeu pue poor PRERE jo 5944 Jo sis amp peue TRUORINNN 1 ddd PUE 44 JO TEAIATNS pue JO VONELINSI UOBLAJOSGO pue Ajsusp pawr JO onelndtueu je usuuiodxg 52920 KISSIRU JA
251. el electricity equipment and consumables for the project activities were provided by and Annual amount allocated by organization is given in the table below The detailed cost is provided in the Annex 11 Unit US dollars n a Data are not available yet 4 Facilities provided by the counterpart organizations Counterpart organizations offer the following facilities for the project activities in the Achotines Laboratory 1 Office space for Japanese experts researchers 2 Research facilities and equipment of the Achotines Laboratory 3 2 Progress of the Planned Activities The project activities have been carried out in accordance with the and PO since the beginning of the Project The project activities undertaken from April 2011 to October 2013 are summarized in the Table below with indications of the level of achievement at the time of Mid Term Review based on the information provided by the researchers involved in the Project 500491011 JO oaos pue sojuon pue sear jo Zune 1 6 ICUTSTX3 30 pue sajuaanf pue jo f JO kBojoqdiow peum xoypeurojur pus JO A3ojoydiour wusu JO sise pue ddd Jo 4 Jo paxds uo PUE JJA jo jo 30
252. enfoque de JICA en Panam 4 Ventaja comparativa de la cooperaci n t cnica de Jap n La Universidad de Kinki de Jap n es una universidad l der en el campo de estudios relacionados con la acuicultura y ha logrado por primera vez en el mundo cerrar el ciclo de vida del PBF La universidad ha acumulado conocimientos y pericias t cnicas importantes en el cultivo de PBF durante m s de 40 a os de trabajos de investigaci n Se puede considerar que la asociaci n con la Universidad de Kinki es una gran ventaja para llevar a cabo estudios comparativos de YFT y BFT en forma eficiente 5 Pertinencia del enfoque tomado por el Proyecto En el Laboratorio de Achotines hay tanques de cr a de poblaci n de reproductores donde el desove de YFT ocurre casi durante todo el a o En comparaci n con instalaciones similares en otras reas del mundo donde se observa el desove solamente una vez por durante algunas semanas el laboratorio estrat gicamente situado en Achotines ofrece un ambiente ptimo para llevar a cabo los estudios de biolog a reproductiva y de ciclo vital temprano de YFT 4 2 Efectividad Se anticipa que el Prop sito del Proyecto ser realizado con efectividad hasta el final del Proyecto Las actividades del proyecto en general han tenido un buen avance y se han producido una cantidad aceptable de resultados de investigaci n seg n indica el n mero de documentos de investigaci n publicados elaborados hasta ahora Sin emba
253. es and a broodstock Currently sardine and squid are used as feed When a higher portion of squid was feed the spawning performance was better From this result and additional chemical analysis it was Suggested that the use of feed with higher polyunsaturated fatty acid HUFA is necessary for broodstock of YFT Initially it was planned to investigate the effects of quantity and frequency of feeding and additives However activities for identifying optimum nutritional condition for broodstock has been suspended because the change of feeding condition may cause a cease of spawning and it may affect other research activities There is a difficulty to conduct research on formula feed development for broodstock because it is necessary to transport ingredients for making the formula feed Although there is limited availability of broodstock the research team will look into the use of trash fish with added phospholipids vitamin E and vitamin which would have positive effects on spawning induction and egg quality Indicator 1 2 At least two optimum environmental factors identified temperature range light intensity photo period moon phase water chemistry for YFT spawning Spawning fecundity spawning time hatchability and environmental conditions weather temperature breeding water temperature etc of YFT have been recorded daily under the captive situation in the Achotines Laboratory Analysis of the collected data will
254. esarrollo Edison Cede o ARAP Director Reglonal de Los Santos ingrid Sanz Sub Directora General de Investigaci n y Desarrollo Nadya Ramos ARAP Relaciones P blicas ARAP j Raul Gonzalez 7 10 Osvaldo Alexis CIAT Director en Panam Coordinadora de Cooperaci n T cnica Maria de las Mercedes Representante del Mnisterio de Economia y 12 Villalaz Finanzas Yoshifumi Sawada Universidad de Asesor Jefe del Proyecto Shunji Sugiyama JICA L der de la Misi n de Evaluaci n Suguru Kubo JICA Planificador de la Evaluaci n Director Elys 23 Esperanza Paniza Embajada del Jap n Representante de la Embajada de Jap n THE JOINT MID TERM REVIEW REPORT ON JAPANESE TECHNICAL COOPERATION SATREPS FOR COMPARATIVE STUDIES OF THE REPRODUCTIVE BIOLOGY AND EARLY LIFE HISTORY OF TWO TUNA SPECIES FOR THE SUSTAINABLE USE OF THESE RESOURCES Panama City November 26 2013 Mr Shunji S Mr Edison Cedefio Leader Leader Japanese Mid term Review Team Mid term Review Team of the counterpart Japan International Cooperation Agency organizations Aquatic Resources Authority of Panama Table of Contents 1 Introduction 1 1 Background of the Project 1 2 Objectives ofthe Mid term Review 1 3 Member of the Joint Review Team 1 4 Schedule of the Mid term Review 1 5 Methodology of the Mid term Review 2 Outline of the Project 2 1 Summary of the Project
255. et on regular basis the functionality of these sub committees may be limited so that complementary measures are needed to resolve this issue 3 5 2 Project Implementation Process 1 Turnover of counterpart personnel of ARAP At the beginning of the Project ARAP exerted considerable efforts to assign counterpart personnel to all the research fields However 6 counterpart personnel were resigned from their posts Although vacant counterpart posts were promptly replaced by others but capacity building of these new counterparts needed to be re initiated 2 Communication among researchers involved in the Project It is reported that communication between Japanese researchers and their counterpart personnel has not been conducted smoothly in some occasions It was indicated that such poor communication might have affected efficient implementation of the project activities 15 Za 3 Advance planning of research work at the Achotines Laboratory At the Achotines Laboratory it is required that research plan is submitted to the administrator of the Laboratory well advance of researchers arrival in order to ensure coordinated use of laboratory facilities and equipment As for researchers duty trip arrangements need to be made since most of them need to travel to the Laboratory from their assigned places As such they need at least 30 days of lead time for application and preparation of their duty trips It is reported that there we
256. f YET and PBF nutritional value of artificial and natural food types of YFT and PBF 1 Visual features and responses to light of larvae and juveniles Comparative studies on the feeding amount growth and the survival rate in breeding larvae and juveniles under different conditions using LED lights which have different wavelengths have been carried out As for the visual feature of larvae of YFT and PBF their similarity was confirmed as a kind of opsin gene sequence is developed largely at the start of feeding It is considered that the response of PBF to a variety of light information is related to the development of opsin gene sequence Response of YFT to light will be Studied in the near future After resume of spawning the experiments on response to light information will be conducted for one year 2 Feeding ecology behavior growth and survival Investigations on attacking behavior and change of survival rates of larvae of YFT have been conducted under different culture conditions such as feeding density and size of individual larvae It has been suggested that factors inducing cannibalism in larvae of YFT are the difference of size and lack of feeding On this aspect similarity with PBF is confirmed However it has been suggested that the degree of influence of these two factors are different in the two cases Detailed study will be conducted the near future Culture experiment of PBF has been conducted to examine starvation toleran
257. f anesthetics among MS 222 2 phenoxyethanol and clove oil yellowfin tuna Thunnus albacares early Juveniles Presentado a Aquaculture Science 12 Terayoshi Tanaka Kenji Takahashi Kohsuke Adachi Haruki Ohta Yukihiro Yoshimura Yasuo Yoshifumi Sawada Osamu Takaoka Amal Biswas Kenji Takii Nobuhiro Zaima Tatsuya Moriyama and Yukio Kawamura Procollagen al 1 gene expression in fish larvae and early juveniles as a novel somatic growth index Presentado a Fisheries Science 13 Teruyoshi Tanaka Tomoki Honryo Yoshifumi Sawada ODaniel Margulies Amal Biswas and Kenji Takii Biochemical changes in Yellowfin tuna egg with embryonic development Presentado a Fisheries Science 14 Toru Kobayashi Tomoki Honryo Yasuo Agawa Yoshifumi Sawada Ollleana Tapia O Karla Adames O Amado Cano Vanon Scholey ODaniel Margulies and Naoki Yagishita Gonadogenesis and slow proliferation of germ cells in juveniles of cultivated yellowfin tuna Thunnus albacares Presentado a Reproductive Biology 15 Naoki Yagishita Yoshifumi Sawada Yasuo Agawa and Toru Kobayashi Isolation and characterization of 25 microsatellite loci from the Pacific bluefin tuna Thunnus orientalis Perciformes Scombridae Presentado a Conservation Genetics Resources 16 Yuichi Tsuda Shinji Yamamoto Hiroshi Yamaguchi Takayuki Ohnishi Wataru Sakamoto Osamu Murata Vertical movement of spawning cultured chub mackerel Scomber japonicus in a net cage Pre
258. f the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology March 2013 Es 8 ATakayukl Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Studies on the behavior and feeding on bluefin tuna larvaey effects of water temperature and food on the swimming speed The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo Universily of Marine Science and Technology March 2013 9 ATakayuki Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Effects of feeding on oxygen consumption and swimming velocity of Bluefin tuna larvae The 2013 spring mceting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology March 2013 10 Michio Kurata Yasunori Ishibashi Tomoki Honryo Shigckazu Katayama Hiromu Fukuda Kenji Takti Shigeru Miyashita Hidemi Kumai Yoshifumi Sawada Dysfunction of swimbladder inflation of Bluefin tuna relationship with growth and lordos s The 2013 autumn meeting of the Japanese Society of Fisheries Science Mie University September 2013 3 Presentation by poster at conferences 1 AShinichiro Hashimoto Tomoki Yasunori Ishibashi Proper light wavelength for Blucfin Thunnus orientalis larvae The conference of the Zoological Soclety of Japan 82 September 2013 in Asahikawa 2 Honryo T Yamamoto H Hirose H Kaze Sawada The effect of EAS dietary fatty acids on survival
259. fin tuna The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology March 2013 13 ANomura 5 Kobayashi Agawa Y OMargulies OScholey V Sawada Y Yagishita Y Genetic population structure of the yellowfin tuna Thunnus albacares the North Pacific Ocean 9th Indo Pacific Fish Conference June 2013 Okinawa June 2013 14 Yasunori Ishibashi Mari Akiyama Michio Kurata Tokihiko Okada Changes in the stress response and low oxygen stress tolerance associated with the development of bluefin tuna larvae The 2013 autumn meeting of the Japanese Society of Fisheries Science Mie University September 2013 15 Awa Yasuo Agawa Tomoki Honryo Michio Kurata Tokihiko Okada and Yoshifumi Sawada Analysis of the HIF l gene transcripts under hypoxia and or hypercapnia in red sca bream Pagrus major carly embryos Afsa Paific Aquaculture 2013 Ho Chi Minh December 2013 16 Yasunori Ishibashi Tomotaka Saida O Yazmin Villarreal OAngel Guillen Yoshifumi Sawada Tomoki Honryo Ontogenic changes in tolerance to high and low temperature stressors of larval and juvenile Pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh December 2013 5 s ee 11542401 55 Peer vorpnpunt Bus p 134 36 pana 5754 212079
260. gas 13 OJcanne Wexler ODaniel Margulies Stein and O Vernon Scholey Collaborative research activities conducted by the IAFTC s Early Life History Group during 2011 2012 Tuna Conference Lake Arrowhead Calif Mayo 2012 154 14 Yasuo Agawa ATakafumi Komiya Tomoki Toru Kobayashi Michio Kurata Tokihiko Okada and Yoshifumi Sawada Identification of a male characteristic DNA marker of pacific bluefin tuna Thunnus orientalis European Aquauiture 2012 Praque Noviembre 2012 15 Yasuo A Komiya Tomoki Honryo Toru Kobayashi Michio Kurata Okada and Yoshifumi Sawada Identification of a male characteristic DNA marker of aquacultured pacific bluefin tuna Thunnus orientalis Aquaculture America 2013 Nashville Febrero 2013 16 ODanicl Margulics O Vernon Scholey OJeanne Wexler OMaria Stein Yoshifumi Sawada Yang Su Kim Tomoki Honryo and OAngel Guillen Comparative research on the reproductive biology and early life history of Pacific bluefin tuna Thunnus orientalis and yellowfin tuna Thunnus albacares Aquaculture America 2013 Nashville TN Febrero 2013 17 Amal Biswas Biswajil Biswas and Kenji Takii The development of formulated diet for promoting sustainable aquaculture of Pacific biuefin tuna Thunnus orientalis BIT s 2nd Annual World Congress of Mariculture and Fisheries Hangzhou China Septiembre 23 235 2013 18 Yang Su Kim ATsukasa Sasak
261. gencia de Cooperaci n Internacional de Jap n Agencia de Ciencia y Tecnolog a de Jap n ATREPS Asociaci n para la Investigaci n en Ciencia y Tecnolog a para el Desarrollo Sostenible At n de Aleta amarilla 103 1 Introducci n 1 1 Antecedentes del Proyecto La pesca del at n es una industria importante para la Rep blica de Panam de aqu en adelante denominado Panam que genera ingresos en divisas valiosos para el pa s La producci n de la pesca se procesa y exporta a los paises de Am rica del Norte y Europa En los ltimos a os los recursos de at n muestran una tendencia descendente y se dice que la presi n de pesca excesiva es una de las principales causas del problema Los recursos de at n son especies altamente migratorias que se distribuyen a lo largo de todo el Oc ano Pac fico y justamente por ello estos recursos son explotados por un gran n mero de pa ses con costas en este Oc ano El at n de aleta amarilla en lo sucesivo YFT y el at n de aleta azul Pac fico en lo sucesivo PBF son las principales especies objetivo de la pesca industrial de at n y Panam es uno de los principales paises productores de YFT El volumen de pesca de at n y especies afines de Panam lo ubican como el tercero m s grande entre los pa ses Latinoamericanos En cuanto a la investigaci n cient fica sobre los recursos de at n Panam es el pa s donde la Comisi n I
262. go de del Coro Lem del Toro Perlas lala San Golfo de Panam e posed 6 PACIFIC OCEAN a ARAP Aquatic Resources Authority of Panama BAC Bacterial Artificial Chromosome DHA Docosahexaenoic Acid DNA Deoxyribonucleic Acid IATTC Inter American Tropical Tuna Commission so Ls on TES JCC Joint Coordinating Committee JICA Japan International Cooperation Agency JST Japan Science and Technology Agency LED Light Emitting Diode M M Minutes of Meeting MEF Ministry of Economy and Finance PBF Pacific Bluefin tuna PCR Polymerase Chain Reactio
263. gth on growth and survival rate in juvenile Pacific bluefin tuna Thunnus orientalis Environmental Biology of Fishes in press 2 Yoshifumi Sawada AToshio Kaga Yasuo Agawa Tomoki Honryo Yang Su Kim Masahiro Nakatani Tokihiko Okada OAmado Cano ODaniel Margulies and O Vernon Scholey Growth Analysis in Artificially Hatched Pacific Bluefin Tuna Thunnus orientalis Aquaculture Science in press 3 Sakamoto Wataru ATakayuki Ohnishi Yuichi Tsuda Effect of food and temperature on swimming behavior of juvenile bluefin tuna Proceedings of 19th International Symposium on Biotelemetry Peer reviewed proceedings in press 4 OMaria Stein Margulies OJeanne Wexler O Vernon Scholey Yang Su Kim Tomoki Honryo A Tsukasa Sasaki and Yoshifumi Sawada Comparative studies of enrichment media for producing nutritionally enhanced prey and their effects on growth survival and cohort biomass of Pacific bluefin larvae Submitted to Marine Ecology Progress Series 5 OAngel Guillen Tomoki Honryo OJuan Ibarra OAmado Cano ODaniel Margulies OVrnon Scholey OJeanne Wexler OMaria Stein Toru Kobayashi and Yoshifumi Sawada Effect of embryonic development of Yellofin tuna Thunnus albacares Submitted to Aquaculture Science 6 Tomoki Honryo Naoki Kaze Hiroshi Yamamoto Hideki Hirose Yasuo Agawa and Yoshifumi Sawada Effect of different dietary fatty acids on survival growth handling stress resistance and lipid metabolic related gene
264. ha asignado once 11 miembros del personal de la como persona contraparte del Proyecto hasta la fecha Parece que el n mero de contrapartes es apropiado La ARAP asign el presupuesto para las actividades del proyecto incluyendo los gastos de viaje combustible y la adquisici n de equipo La ARAP ha hecho esfuerzos sustanciales para asignar presupuesto sin embargo el monto actualmente asignado para las actividades del proyecto no ha alcanzado el nivel al cual la ARAP se hab a comprometido al inicio 3 Insumos por la CIAT En t rminos del aporte financiero al Proyecto la est cumpliendo con sus compromisos Referente a la asignaci n de personal contraparte el director y dos investigadores del Laboratorio de Achotines han participado en las actividades del proyecto y tres investigadores de la sede de la CIAT en California EE UU han visitado el Laboratorio de Achotines y llevado a cabo sus investigaciones Adicionalmente tres investigadores de la sede de CIAT han visitado la Universidad de Kinki en la estaci n de desove de PBF y llevado a cabo investigaciones relacionadas con PBF Parece que los insumos dados por la son apropiados El problema de la coordinaci n de las actividades de investigaci n mencionado anteriormente tambi n fue relevante en el caso de los investigadores de CIAT debido a que sus visitas al Laboratorio coincidieron con las visitas de los investigadores de la Universidad de Kinki 4
265. hat improvement of success rate from capture to healthy YFT broodstock with this indicator will be completed within the project period The survival rate from capture to handling of YFT of 1996 2000 was 50 9 in average Indicator 4 5 At least 25 YFT juveniles of 20 cm produced after 3 month period in ocean cage Installation of ocean cage has not been completed Therefore investigation related to this indicator rearing juveniles in ocean cage has not started The installation of ocean cage is scheduled in February 2014 Rearing of YFT in ocean cage will be carried out from the spring of 2014 If broodstocks of YFT of the Achotines Laboratory resume spawning by the spring 2014 it is possible to be completed the research activities using ocean cage in the remaining two years Indicator 4 6 Visceral organ development described for larval and juvenile YFT It was planned to start investigation on development of internal organs or viscera organs by using larvae and juveniles produced in 2013 Hawever spawning of the VFT broodstock of the Achotines Laboratory halted in 2013 Therefore samples necessary for investigation were not obtained After the resume of spawning this investigation will be started Duration necessary for carrying out this investigation is around one year therefore it is expected description of visceral organ development with this indicator will be completed during the project period 3 4 Project Purpose Project Purpos
266. i Masato Awa AMaho Inomata Tomoki Yasuo Masashi Ando and Yoshifumi Sawada Effect of dietary taurine enhancement on the growth and ontogenetic development red sea bream Pagrus major larvae Asia Pacific Aquaculture 2013 Diciembre 2013 19 Yasuo Agawa Yoshihiro Takada Kohei Tamura AMasato Awa Tomoki Honryo Toru Kobayashi Michio Kurata Okada and Yoshifumi Sawada Identification of male and female characteristic DNA marker of aquacultured pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh Diciembre 2013 20 Tomoki Honryo Michio Kurata Ai Sumitomo and Yoshifumi Sawada The explore of possible initial swim bladder inflation periods and time of day on Pacific bluefin tuna Thunnus orientalis World Aquaculture Society 2013 Ho Chi Minh Diciembre 2013 21 Tomoki Honryo O Angel Guillen Teruyoshi Tanaka O Amado Cano ODaniel Margulies O Vernon Scholey and Yoshifumi Sawada Effects of water surface condition on survival grawth and swim bladder inflation ration on yellowfin tuna Thunnus albacres larvae Asia Pacific Aquaculture 2013 Ho Chi Minh Diciembre 2013 22 Yoshifumi Sawada AToshio Yasuo Agawa Tomoki Honryo Yang Su Kim Michlo Kurata Yasuhiro Nakatani Toshio Tamura Tokihiko Okada and Shigeru Miyashita Growth analysis in artificially hatched Pacific bluefin tuna Thunnus orientalis Aisa Paific Aquaculture 2013 Ho Chi Minh Diciembre 2013
267. i n toman el papel de liderazgo la iniciativa en la organizaci n las actividades de investigaci n dentro de propias organizaciones y conversan o consultan con las dem s organizaciones para lograr un trabajo de investigaci n bien preparado y coordinado Tal coordinaci n a nivel de organizaciones es particularmente importante en este proyecto debido a que las instalaciones de investigaci n por ej los tanques de cr a y 20 123 Lo equipo de an lisis y materiales huevos y larvas de YFT y alimentos en el Laboratorio de Achotines frecuentemente se vuelven escasos durante la poca pico del trabajo de investigaci n Cuando la comunicaci n de persona a persona entre los investigadores falle los Coordinadores de Investigaci n pueden intervenir para facilitar la comunicaci n 3 Intercambio de informaci n entre investigadores En el Proyecto los investigadores est n agrupados en tres reas tem ticas y existe la tendencia a que el flujo de la informaci n est algo confinado dentro del mismo grupo de investigaci n A fin de facilitar el intercambio y uso compartido de la informaci n inter e intra grupales dentro del grupo y con otros grupos se recomienda que se organicen eventos pata compartir la informaci n tales como seminarios o talleres internos para el personal del propio proyecto En estos eventos se pueden presentar la planificaci n y hallazgos de las investigaciones y tecnolog as relacionadas Tales event
268. ign Matrix PO Plan of Operation R D Record of Discussions SATREPS Science and Technology Research Partnership for Sustainablo Development YFT Yellowfin tuna Ec 1 Introduction 1 1 Background of the Project Tuna fishery is an important industry for the Republic of Panama hereinafter referred to as which generates valuable foreign exchange earnings for the country The production from the fishery is processed and exported to the North American and European countries In recent years tuna resources show a decreasing trend and the excessive fishing pressure is said to be a major cause of the problem Tuna resources are highly migratory species distributing widely in the Pacific Ocean and because of this nature these resources are exploited by a large number of coastal countries of the Ocean Yellowfin tuna hereinafter referred to as and Pacific bluefin tuna hereinafter referred to as PBF are major target species of the tuna fishing industry and Panama is one of the major producer countries of YFT The volume of tuna and tuna like species catch of Panama is the third largest among Latin American countries In terms of scientific research on tuna resources the Panama is the country where the Inter American Tropical Tuna Commission IATTC decided to establish its research facility the Achotines Laboratory It is the only research station of regional fisheries bodies where YFT brood
269. imilitud de que una tipo de secuencia de gen de la opsina se desarrolla en gran medida al inicio de la alimentaci n Se considera que la reacci n del PBF a una variedad de informaci n de luz est relacionada con el desarrollo de la secuencia del gen de la opsina La reacci n del YFT a la luz se estudiar en un futuro pr ximo y posteriormente se identificar la similitud y la diferencia Despu s del re desove de los reproductores se completar n en un a o los experimentos sobre la reacci n de informaci n de fuz 2 Ecolog a de alimentaci n comportamiento crecimiento y supervivencia Se ha llevado a cabo las investigaciones sobre el comportamiento de ataque y el cambio de las tasas de supervivencia de las larvas del YFT en diferentes condiciones de cr a tales como la densidad de alimentaci n y el tama o de las larvas individuales Se ha sugerido que los factores inductores de canibalismo en laryas de YFT son la diferencia de tama o y falta de alimentaci n Se confirma esto en la similitud en el caso de PBF Sin embargo se ha sugerido que el grado de influencia de estos dos factores es diferente en los dos casos Se llevar a cabo un estudio detallado en un futuro pr ximo para ordenar un poco estas sugerencias Se ha sugerido que si se producen estos dos factores al mismo tiempo entonces 12 se producir ef canibalismo frecuente tanto en el YFT como en el PBF Se llev a cabo un experimento de cr
270. ino ysy 3urseuo 40 SPORU 1224 JO SIS TETE 105 2101140 10129102 2389 vos 24 UE SSERT9AT LH Jo Surreo 0 adeo pue Zurgiurad URIS 3116 Zp LAA Jo soono ageo pue AI9UQIBT jo LIA JO EAIAINS pue ToI BATE Jo 54 pue pue sumpoa Ardens 1378 33 JO 1 JO 2 pue Mau SIT 2 LAA JO tonepodsuen pue jo uopuogipour pue E p 10 xpoispoo1q pue HS USE s dures onssm JO SIS BUE 1 7 1201 LFA 107 spoureur uonguodsue pue mdgo jo LIA JO Waun gereu TY 10g Jo 2 Z lt 3 3 Outputs 3 3 1 Output 1 Spawning characteristics of YET and PBF are determined Indicator 1 1 At least two optimum nutritional condition diet composition quantity and frequency of feeding and supplements of YFT broodstock identified For this Project it is difficult to conduct a precise comparative test because of limited availability of rearing tank faciliti
271. ir works at respective organization 3 Financial Aspect The budget allocation plan for the Project was agreed among ARAP LATTC and JICA at the first JCC meeting It is important to adhere to the agreement for ensuring financial resource in a cooperative manner 4 6 Conclusions The Joint Mid term Review Team has confirmed that the project activities have shown a good progress in general As the results of the project activities scientific knowledge and information on early life history spawning ecology and nutrition have been produced as originally planned The summary of evaluation based on five evaluation criteria is described in the table below Efficiency Moderately high Corrective actions are needed to cover lost time in ocean cage installation Countermeasures are needed to deal with current halt in spawning Impact Premature to assess Only provisional impact is described Sustainability Likely to be high On the condition that trained counterpart personnel remain in the organizations 5 Recommendations Issues relating to the implementation procedure 1 Improved communication among researchers In this project a large number of researchers from three different counterpart organizations are involved to conduct joint research and many researchcrs visit the Achotines Laboratory on a short term basis Such nature of research work inevitably requires communication by e mail as face to face meetings a
272. ity Septiembre 2011 2 AShohei Nomura Toru Kobayashi Yasuo Agawa Dan Margulies Vernon Scholey Yoshifumi Sawada Naoki Yanagishita Genetic Population Structure of Pacific Bluefin tuna and Yellowfin tuna in north Pacific Ocean The 2012 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University Septiembre 2011 3 Yang Su Kim AMaho Inomata AMasato Awa ATsukasa Sasaki Tomoki Honryo Yoshifumi Sawada Effect of rotifers enriched with taurine on growth and transformation of red sea bream larvae The 2012 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University Septiembre 2012 4 ATakayuki Onishi Yuichi Tsuda Wataru Sakamoto Shinji Yamamoto Osamu Murata Effects of temperature and food on the survival of cultured bluefin tuna larvae The 2012 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University Septiembre 2012 5 Yoshifumi Sawada Tomoki Honryo Yasuo Agawa AKazuhiro Higuchi AMasato Awa OAmado Cano O vemon Scholey ODaniel Margulies Teratogenic environmental factors to fish embryo low CO2 and high CO2 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 6 Toru Kobayashi Tomoki Honryo Yasuo Agawa Yoshifumi Sawada I Adames K OCano A Scholey V OMarguries D Naoki Yanagishita Gonad formation process of cultured yellowfin tuna
273. iva de tres a cuatro t cnicos deben ser asignados para la gesti n de la jaula dado que se requiere renlizar trabajos diarios de operaci n y mantenimiento tales como alimentaci n de peces y recolecci n de peces muertos Se recomienda que la ARAP asuma un papel de liderazgo para ocuparse de este asunto FIN 22 125 e c SONO TQIS Y SOIQUIS 00 61 UOISHA v SDIQUIAA LONMIY 00 1 y ap SXIETed 00 91 i opeynsoy vpeo somu op AIN SalopeShsaAnT 4 2 OPIJA 00 HI 0 21 IVIO 7 1 4 0 00 11 LVIO sodmbe sauojzejegsu ugroeAmsQO 06 60 LVIO 987 1015514 e ets HoO 00 60 TVIO 1 00 60 00 80 E 50 uoo 00 spuodef e 00 61 Opel Y soqua soj uoo 00 61 2 ope seJg 00 51 SSUROYSY epeaorT 00 61 d viIV sunfuoo AON 81 00 51 VJ somur rm SO 109 UOIUNSY 00 61 uoroene4g ap s uoo ugmme 00 21 uoo uornme 00 71 AYAV EXSALIOS 3p DISIA 00 11 AVV FISIA 00 11
274. ized Achievement It is expected that the research results will be compiled in a synthesized form and disseminated by means of three media publications website and regional seminars workshops It is also expected that the research results will be presented at regional and international seminars which will be held before the end of the project term In addition biological information will be compiled in a book of which publication can be after the termination of the Project 2 Summary of Evaluation Results 1 Relevance High Tuna fishery is an important industry for the Central American countries including Panama which constitutes an important part of foreign currency earnings A declining trend of tuna catch is evident in the Pacific Ocean and it is necessary to take prompt conservation and management actions Therefore this project is consistent with needs of improving resource management of tuna species As for the fishery related policies of Panama sustainable management of marine resources and development of aquaculture are important basic principles and promotion of research on YFT is also regarded important as because of its high potential for aquaculture development Therefore the objective of the Project is in line with above mentioned Panama s policy objectives One of the important sectors for JICA s assistance work in Panama is environmental conservation which naturally includes conservation of the natural environment and
275. length for survival and growth performance and analysis of the opsin gene expression in the larvae of Pacific bluefin tuna Thunnus orientalis Asla Paciflc Marine Biotechnology Conference APMBC Kochi July 2012 8 Tomoki Honryo Guillen OJuan Ibarra ODaniel Margulies O Vernon Scholey Toru Kobayashi and Yoshifumi Sawada Temperature devendent embryonic development of yellowfin tuna Thunnus albacares European Aquaculture Society AQUA2012 Praque September 2012 9 Yang Su Kim Chihiro Aoki Shinji Yamamoto Kenta Ishimoto Kenji Takii Osamu Murata and Yoshifumi Sawada Effect of starvation on survival rate and body composition of juvenile pacific bluefin tuna Thunnus orientalis European Aquaculture Society AQUA2012 Praque September 2012 10 Yang Su Kim O Daniel Margulies Vernon Scholey Amado Cano and Yoshifumi Sawada Investigation of suitable water temperature and salinity for hatching and early larval stages of yellow fin tuna World Aquaculture Sejety 2013 Ho Chi Minh December 2013 11 Teruyoshi Tanaka Tomoki Honryo Yoshifumi Sawada Vernon Soholey Amal Biswas and Kenji Takii Gross energy consumption in Yellowfin tuna with embryonic development Aquaculture 2013 Nashville Tennessee USA February 22 25 2013 12 Toru Kobayashi Tomoki Honryo Yasuo Agawa Yoshifumi Sawada O Tapia O Adames K OCano A OScholey V OMarguries D Naoki Yanagishita Gonad formation process of cultured yellow
276. licitud fue hecha debido a que los contrapartes de la CIAT no cuentan actualmente con apoyo financiero relacionado con este proyecto para este tipo de viaje EIN Ap ndice 1 Lista of participantes Ap ndice 2 Informe de la Revisi n Intermedia Conjunta Ap ndice 3 Plan de Operacion Versi n 2 aS VES Ap ndice 1 LISTA DE PARTICIPANTES REUNI N ALMUERZO COMIT CONJUNTO DE COORDINACI N E INFORME DE EVALUACI N INTERMEDIA DEL PROYECTO PRO AT N Fecha 26 de noviembre de 2013 HORA 9 00 a m 2 30 p m Lugar Hotel Continental Ciudad de Panam No Nombre Imstituci n Cargo Nely Serrano ARAP Secretaria General 2 Franklin Kwal Ben ARAP Director General de Investigaci n y Desarrollo ARAP Director Regional de Los Santos Sub Directora General de Investigacion y Desarrollo Relaciones P blicas Director Administrativo Lider C P Biologia Reproductiva B Director Laboratorio Achot nes Asesor Jefe Senior Scientist Osvaldo Alexis CIAT MEF Coordinadora de Cooperaci n T cnica de las Mercedes Representante del Mnisterio de Economia y Villalaz MEF Finanzas Yoshifumi Sawada Universidad de Kinki Asesor Jefe del Proyecto Shunji Sugiyama ICA L der de la Misi n de Evaluaci n 16 lisao Dojun ICA Shoji Kibe JICA Coordinador de Proyecto David Kanagy independiente Int rprete 19 Kazumi Kobayashi JICA Panam Director 20 Ayumi Takeba
277. lipids are consumed mainly as energy source for egg devclopment while consumption of proteins is maintained to be minimum From these findings it is suggested that an important indicator to determine the egg quality is egg lipid Development of biochemical assay method for determining egg quality of YFT will be started after resume of spawning of YFT broodstock and it is expected that a biochemical assay methad will be developed within the project period 3 3 2 Output 2 The method to identify maternal line of YFT is established by using mitochondrial D loop for analysis Indicator 2 1 Primers identified to detect the polymorphism of mitochondrial D loop As for YFT fins of YFT were collected at the fish market near the Achotines Laboratory samples were taken from those fins and analyses were carried out As for PBF samples were collected from 500 individuals of PBF which were obtained from the fish market in Japan and analyzed After a series of experiments in this project primers to detect the polymorphism of mitochondrial D loop were identified and this component has been achieved Indicator 2 2 Independent maternal lines of YFT identified As for YFT samples were collected from 25 individuals of YFT caught in Japan and 77 individuals of YFT landed in Panama The results of analysis indicated that genetic diversity was wide but dominant populations were not found However in order to verify these results other methodologies will
278. lm sanaw LIE sa ES ENO SY 325 5294001 pesodstp 4477 suede wa mt RIA JE 3 9201210 59005 LING MIU 458 2212 21 one om s x e 1 re 0DSONd apes Ao V mons aro veder aaa a rt m m p 01381 umma a AA je A ets a t f meder meder mi oem dumd mder 001 apquszousqns yews er 66 192100131 BED 48906 IIN DIO 8 218 E JBE OSI 951 8 N D e a 318 2 lt jw 5 818 5 E E 8 8 a 2 lt L6 orior som ssunogoy vureue 56 sm 8 8 4 8 8 Ex N Decr 252 jore att Peni JT E a er pa eee Saca 2007 sen ssn a 4 1007 ve voy oz NpnO umu puz IPO suey ae R sola EEE 5 99 5
279. mado Cano Dr Lissette Trejos Ms Anna Nufiez Ms Yazmin Garcia Ms Liliana Guerra Ms Diana Perez Mr Alvaro Diaz Ms Ileana de Tapia My Giancarlo Cerrud Mr Angel Guillen Deputy General Administrator General Secretary Director General of Research and Development Deputy Director of Research and Development Director of Administration and Finance Researcher Achotines Laboratory Dept of Research Directorate General of Research and Development Dept of Research Development Directorate General of Research and Development Dept of Research Directorate General of Research and Development Vacamonte Experimental Station Vacamonte Experimental Station Divisa Experimental Station Aguadulce Experimental Station Aguadulce Experimental Station Vacamonte Experimental Station 2 IATTC Dr Daniel Margulies Dr Vernon Scholey Mr Luis Tejada Ms Susana Cusatti Senior Scientist Early Life History Studies Coordinator Headquarters in USA Laboratory Director Achotines Laboratory in Panama Achotines Laboratory Achotines Laboratory 3 APR Biswas Amal 4 5 JICA
280. mente se report un caso de p rdida de un art culo y se requiere prevenir que tal incidente ocutra otra vez Al respecto se recomienda que la gesti n de los equipos e instrumentos del Proyecto sea fortalecida a fin de garantizar la utilizaci n de los mismos 11 Consideraci n de contramedidas por la paralizaci n prolongada del desove Se prev que el desove de YFT posiblemente 52 reanudar alrededor del mes de abril de 2014 cuando la temperatura del mar aumente y la demora de algunas actividades causada por la actual paralizaci n en el desove tambi n se solucionar Por el momento las medidas tomadas sobre esta cuesti n son principalmente un estrecho monitoreo de los factores ambientales de los tanques de cultivo de reproductores Considerando la naturaleza de las actividades del Proyecto sujetas a un plazo establecido la prolongaci n de la paralizaci n en el desove afectar a seriamente el logro del Proyecto Por lo tanto se sugiere que los investigadores interesados en este tema consideren con prontitud opciones viables de contramedidas en el caso de que la paralizaci n del desove contin e m s all de abril de 2014 12 Instalaci n y gesti n de una jaula marina La instalaci n de la jaula marina se ha retrasado por algunas razones t cnicas Dado que es un componente importante del Proyecto se har n todos los esfuerzos para garantizar que la instalaci n de la jaula sea terminada tan pronto como sea posible En la etapa operat
281. mmu SOYI SOJ VOJIESHSIAUT LAA onb s egu rqtue op uotogans AuT SE erp un LAA ap YOH e op ci H SOPEPIAIPY 169 CiPoT Jaye pz Jo SIMANT LAA LJA o amideo ALI 5592215 0005 9661 JO JO Z 1A JO suodaryeloig e LIA JO Aloysty A 1eo ur SISIB LJA Jo Aos sieded 201109125 42035 poouq 107 9UO 2 e poddns y sergo ouyas uononpoid y Add PYE LAA JO 3904 poo penen pue jo ene Jad pue LAA Jo eAtAums pue TAO 5 Supay Add PUR LAA uonguuojui 181 o suods r re IY PUE UOISIA pue peqnosap LAA eruean pue Jo jueurdo eAep pue e sodai pofoig SJOJ08 pue eors amp ud osiy e OY IYHUADS
282. motion of aquaculture in Panama It is generally said that the knowledge accumulated and techniques acquired by the Project are highly applicable to farming of other marine species These include technical fields of seed production hatchery management fingerling rearing feed development ocean cage management and genetic analysis It is expected that counterpart personnel who work on these fields contribute to the future development and lt 18 promotion of aquaculture in Panama 4 5 Sustainability Sustainability of the Project is likely to be high based on the facts described below 1 Policy Aspect As mentioned in the Section 4 1 achieving the objcctive of the Project will contribute to sustainable management of marine resources and the development of aquaculture in the medium to long term These areas are considered as important issues by the Government of Panama Therefore policy sustainability of the Project will be secured 2 Organizational and Technical Aspcct and JATTC have carried out the joint research since 1985 at the Achotines Laboratory and IATTC is responsible for the conservation and management of tuna and other marine resources in the eastern Pacific Ocean Therefore it is very likely that this joint research structure continues in the mid to long term For ensuring technical sustainability it is fundamental that the counterpart personnel who have acquired related knowledge and technologies continue the
283. muepoduics uorejueurge ei3o ooa e ZN Ble Add A LAA SRAI uo owas E SEJ 1 opor orpruse ue Xode enb sourAo e ep SerSOHOU23 Se 38 opad sepgognuapt SBIOUaJSTIP 5 527 ddd LAA wed s pu anf searey 8 oue1durm SEo 0 50250 TEJA Opo ns 03 Jgd A LAA 5 S21O1OB SOT UQIIB 91 SOTUOSep LAA 9p siseusdoriquie 0522014 A anb soon SO eS opakoid Jep jo SEO HUSIO LAA SEIM dooF osn S9ABN LJA Souoroponqnd e Pupuooognu dooj q 19 pyp seaun sep ered Ja 20514252 ddd LAA Soaany 14 4 LAA vwd ene jap pomnmb un osez Zn peprsueju
284. n PDM Project Design Matrix ENS PO Plan of Operation R D Record of Discussions SATREPS Science and Technology Research Partnership for Sustainable Development 7 WCPFC Western Central Pacific Fisheries Commission YEFT Yellowfin tuna 1 2 4 42 4 000 2011 4 1 ARAP 2016 3 31 IATTCO 1 1
285. n Tonelado em pms ApmsqpeuogumN APms HTA Pue 209095 JOSEAPBJSIHO spadxe una 11045 2 1 1091014 uno Suo T sp dxq Jo ynedsiq 1 apIs oseueder sjndur LAA 10 5 Jo Aiueub pue amp pue pue TBTS9SLA JO 3uouido oAap 9 Jo uonesnsoAup LAA Jo vononpoud 10 2130 00 53 ammo pue Jo 42015 LIA 10 spoyaw uongyodsueg pue jo LAA JO 1 pesg jo LAX Jo pue SrsA gue 209128 51001 Jo 11ourdo oAo q Add pue LAA Jo 244 poog rempu pue ELOY IPE jo euoninnu Jo 523815 ay pue LIA JO TeArams pue ymo Asoo Supay Jo Add LIA ur 3481 asuodsax BAIR UOLIA eu Jo Pe LAA uo siop Jeorureqo pue peorsyd jo sq pue Awa Jo uonp ns Aur SOUT eururexe 5 LAA 193n80 pra papas Aq et JO
286. naka Kenji Takahashi Kohsuke Adachi Haruki Ohta Yukihiro Yoshimura Yasuo Agawa Yoshifumi Sawada Osamu Takaoka Amal Biswas Kenji Takii Nobuhiro Zaima Tatsuya Moriyama and Yukio Kawamura Procollagen al I gene expression in fish larvae and early juveniles as a novel somatic growth index Submitted to Fisheries Science 13 Teruyoshi Tanaka Tomoki Honryo Yoshifumi Sawada O Dantel Margulies Amal Biswas and Kenji Takii Biochemical changes in Yellowfin tuna egg with embryonic development Submitted to Fisheries Science 14 Toru Kobayashi Tomoki Honryo Yasuo Agawa Yoshifumi Sawada Ollleana Tapia Adames Amado Cano OVanon Scholey ODaniel Margulies and Naoki Yagishita Gonadogenesis and slow proliferation of germ cells in juveniles of cultivated yellowfin tuna Thunnus albacares Submitted to Reproductive Biology 15 Naoki Yagishita Yoshifumi Sawada Yasuo Agawa and Toru Kobayashi Isolation and characterization of 25 microsatellite loci from the Pacifie bluefin tuna Thunnus orientalis Perciformes Scombridae Submitted to Conservation Genetics Resources 16 Yuichi Tsuda Shinji Yamamoto Hiroshi Yamaguchi Takayuki Ohnishi Wataru Sakamoto Osamu Murata Vertical movement of spawning cultured chub mackerel Scomber japonicus in a net cage Submitted to Aquaculture 17 ATakayuki Ohnishi Yuichi Tsuda Wataru Sakamoto Osamu Murada Kenji Post feeding changes in oxygen consumption and swimming spee
287. nce of their visit to the Laboratory and share it with research coordinators and their counterparts 5 Improvement of counterpart training in Japan The importance of advanced planning and documentation of information for consultative implementation is also applicable to counterpart training in Japan Close consultation with counterpart personnel on their training plan is required in order to improve the effectiveness of such training The assignment of a supervisor for each training participant is also recommended who is responsible for ensuring overall quality of the training lt Issues relating to the project administration 6 Official assignment of a Linison Officer In this project administrative support rendered by the headquarters of is essential for smooth implementation of the Project In order to ensure continuation of this function it is advisable that ARAP assign a Liaison officer as the technical focal point for the Project who is solely responsible for inter alia communication with the Project reception follow up of document application processing and dealing with other administrative matters of the Project 7 Continued effort to ensure assignment of ARAP counterpart Despite of logistics difficulties ARAP counterparts have to travel to Achotines from their assigned areas ARAP counterparts have been playing important roles in conducting various research activities ARAP efforts to maintain this
288. ne for Farmed Seafood March April 2013 pp 72 73 5 Margulies O Vernon P Scholey OJeanne Wexler OMaria 8 Stein Richard Deriso and Guillermo A Compean The Achotines Laboratory a world leader in tuna research INFOFISH International 2013 pp 24 28 6 P Scholey ODaniel Margulies OMaria 5 Stein Guillermo A Compean Wexler Richard Deriso Cria de atunes El laboratorio de la CIAT en Achotines Panama INFOPESCA INTERNACIONAL NUMERO 52 OCTUBRE DICIEMBRE ANO 2012 26 29 7 Margulies OVernon Scholey OJeanne Wexler and OMaria Stein Achotines Laboratory home to continuing studies of tuna early life history Global Aquaculture Advocate March April 2013 72 73 8 O Maria Stein O Daniel Margulies O Vernon Scholey and Jeanne Wexler El Laboratorio de Achotines atunes aleta amarilla cautivos en Panama Panorama Acuicola 18 3 26 32 9 OJeanne Wexler O Daniel Margulies O Vernon Scholey and O Maria Stein El Laboratorio dc Achotines Panama Fishing Magazine March 29 2013 6 8 10 O Daniel Margulies O Vernon Scholey OJeanne Wexler O Maria Stein Richard Der so and Guillermo Compe n Cria de atunes el laboratorio de la CIAT en Achotines Panama INFOPESCA Internacional 52 26 29 11 OVernon Scholey ODaniel Margulies OJeanne Wexler and Stein Achotines Laboratory Captive culture of yellowfin tuna Thunnus albacares for
289. nne Wexler and O Vernon Scholey Comparative studies of feeding dynamics and growth of yellowfin tuna Thunnus albacares and Pacific bluefin tuna Thunnus orientalis larvae 2013 Tuna Conference Lake Arrowhead Calif May 2013 25 ODaniel Margulies O Vernon Scholey OJeanne Wexler and OMaria Stein Studies of tuna early life history conducted at the ATTC s Achotines Laboratory 2012 2013 2013 Tuna Conference Lake Arrowhead Calif May 2013 26 OVernon Scholey ODaniel Margulies OJeanne Wexler and OMaria Stein Studies of tuna early life history conducted at the Inter American Tropical Tuna Commission IATTC Achotines Laboratory 2012 2013 2013 Larval Fish Conference Miami FL June 2013 2 Presentations in the conferences in Japan 1 AYoshiki Tsutsumi Taro Matsumoto Tomoki Yasuo Agawa Yoshifumi Sawada Yasunori Ishibashi Impact of the various LED light source wavelength in electric illumination breeding on the breeding performance of juvenile bluefin tuna The 2011 autumn meeting of the Japanese Society of Fisheries Science National Fisheries University Kyoto University September 2011 2 AShohci Nomura Toru Kobayashi Yasuo Agawa Dan Margulies Vernon Scholey Yoshifumi Sawada Naoki Yanagishita Genetic Population Structure of Pacific Bluefin tuna and Yellowfin tuna in north Pacific Ocean The 2012 autumn meeting of the Japanese Sooicty of Fisherics Science National Fisherics University September 2011
290. nteramericana del At n Tropical CIAT decidi establecer su centro de investigaci n el Laboratorio Achotines Es el nico centro de investigaci n de organismos regionales de pesca donde se mantienen reproductores YFT y el desove se observa en cautiverio Se llevan a cabo diversas actividades de investigaci n en el Laboratorio Achotines en estrecha colaboraci n con la Autoridad de Recursos Acu ticos de Panam en lo sucesivo ARAP Bas ndose en los antecedentes descritos anteriormente el Gobierno de Panam hizo una petici n al Gobierno de Jap n para llevar a cabo la cooperaci n t cnica cient fica sobre los Estudios comparativos de la biolog a reproductiva y el ciclo vital temprano de dos especies de at n at n de aleta amarilla y at n de aleta azul del Pac fico para garantizar el uso sostenible de estos recursos en adelante denominado el Proyecto El proyecto se inici en abril de 2011 como un proyecto de 5 a os 1 2 Objetivos de la Revisi n Intermedia 1 Confirmar el progreso y los logros del proyecto bas ndose en la Matriz de Dise o del Proyecto y el Pian de Operaciones PO e identificar los factores que les favorecen inhiben 2 Analizar y evaluar el Proyecto desde el punto de vista de los cinco 5 criterios de evaluaci n es decir relevancia eficacia eficiencia impacto y sostenibilidad y 3 Hacer recomendaciones sobre las medidas que deben tomatse durante la segunda mitad del
291. o Agawa Effects of photoenvironmental contro on the feeding growth and survival rate of juvenile Pacific bluefin tuna Thunnus orientalis Asia Pacific Marine Biotechnology Conference APMBC Kochi Julio 2012 7 AShinichiro Hashimoto Tomoki Honryo Yasuo Yoshifumi Sawada Taro Matsumoto and Yasunori Ishibashi Determination of the optimal light wavelength for survival and growth performance and analysis of the opsin gene expression in the larvae of Pacific bluefin tuna Thunnus orientalis Asia Pacific Marine Biotechnology Conference APMBC Kochi Julio 2012 8 Tomoki Honryo O Angel Guillen OJuan Ibarra ODaniel Margulies O Vernon Scholey Toru Kobayashi and Yoshifumi Sawada Temperature devendent embryonic development of yellowfin tuna Thunnus albacares Europcan Aquaculture Socicty AQUA2012 Praque Septiembre 2012 SS ec 9 Yang Su Kim Chihiro Aoki Shinji Yamamoto Kenta Ishimoto Kenji Takii Osamu Murata and Yoshifumi Sawada Effect of starvation on survival rate and body composition of juvenile pacific bluefin tuna Thunnus orientalis European Aquaculture Society AQUA2012 Praque Septiembre 2012 10 Yang Su Kim ODaniel Margulies O Vernon Scholey Amado Cano and Yoshifumi Sawada Investigation of suitable water temperature and salinity for hatching and early larval stages of yellow fin tuna World Aquaoulture Sciety 2013 Ho Chi Minh Diciembre 2013 11 Teruyoshi Tanaka Tomoki Honryo Y
292. od 2443 8 525 146 86 826192 pL EPS IS 590 1 8040197 94461 9606 0000294 00062 000017 9007051 Scy ET ESG E 9070082 5969 pL PTET 160151 900 3 I sosouodef so reuororgnu stsr zug a ered 501059 oin ipu ep ugrsuedxo Seurogoy orojeoqe rep ONP 9p uorsuedx sofeqey sor esed sosen ap sofeqen 104 sojser 00008201 031109 Ugtotorunuroo 01588 se errogeu odmbs op e1ed 501528 504529 sodinba ap uoiorsinbpe ey Bed 501578 sodmbo sp 501520 jeuosied op uos onb seuoTot1ounurow 02192 alesed 5 501587 S e 9 v Jopeurp1oo 10d jap 50115 SO e safeta 501505 op S 5918207 5901580 e Q 93164 vp oprumsy 890 0150 8 147 T To E SA E Sys 00 T E F PRE TOY IA mates RTE uray o o tara ava o O ls RR ee aa A a A m j LI
293. oefoaq 170 Suojmpuoo o1d 3 e oud ayy 24 Aressaceu sasuadxo SuneiadgQ Jeuuosrod yediajunos Jo sasuadxa jouuosieg jueuxdmbe yofard JO pue 1509 8907 p go 32e o1d Joy sued areds pue sslqgumsuoD jueuidmb3 suedxe asaueder ogds WO SIUE uo Serpms 32efo1d a Jo pjoy 22114231 yaxa 21405134 sdajnmo HO1B9S3S IIPUEJA 0 904 Jouuos1ed syimdr junoo Jo JuSuru9Issv 71 TPUIUISS uodxe pony Sumoddng 1509 8207 b 01 ux ouuos1od edemo ueragureueq TO Summi SEO pue pue JO UOISIAO1d Z Seo en uonejedo HTA TeuonmmnN pms HTA Pue n u 8 2 JojguIpiooO pelod suadxo wry 8107 1 asaueder jo qojedst 1 apis osauede LAA sp23J Jo muenb pue pue pue 502820 TRISISTA JO jusurdo oAep a Jo uonesns Au LAA
294. ogu dooj q op usuexq 1 2 SE Opuo n ur SISA 50002810 Jad LJA ap SS raAn seam sarojonpordal 2224 0218010150 Opujse eed ojepdwoo o rouss un o oueseqp p IIA 19 59 ep Sowa ep ugroeSnso u 6 1 LAA jo wJ S210306 8 T I 9AOSgD SE A un emp 141 9p PIO er SP 0012280523 1 1 1401 SIPEPIADO 9024151 ns sed LIA iousArAIsdns 21 ype onb 5052498 saryog og SISI BUE u enpuooonu dooj q ap osa SAVI LAA Scam sej 1e3griuop emd opojeui 552148152 25 484 4 LAA SD ap SBDNS ISIDTILO se og sope19ds7 110 UO9t229 0991 ugroenujuo LIA 2158 UOJONQUOSIP 259 PJ 91511855 tib Sapeplanoe ap S DEDEAT P ap CO O QE SBHT31qoxd 505 1405 A vureueq ua sopioupoud sojuaur e opupsn 5
295. ol Loci Dua O wie 0001 woder 0001 46 oxy aemoqreohIodT000I weder euroqreoKjod 0001 55 ms ameo 4 9POE goreaseu 1098108 e ato gt a A E ao ia wer PRS DU suoyequou paja warme 897 o 8 1 059T 00085 00085 x t e p onfireg exeue SE AS 143 m 68 x 0985 z Qtr X 00195 amp 1 82 900 10 iusto weder gm 3 1 CAN El Kk 0 E i d k w 111876 sis eue sprae onime 10 5119892 HOMIIV VADIS 82292010 SisArere spice sudo NIYVDYA OINV3I 264 sus jeuo maoud 5119997 945906 515 HORKTV VWDIS 624800 SISAIEUE sued HORXTIV VADIS E o ZO E HORITIV VINOIS gag wridsongl upder we maaa quaSeas Jou ud s n ll9012 2 fest epmexp qumspog uder 352 apap ele umpos 06171 1 NE e NEM 51400
296. ombea mereven Jom ouea 0958 09992 R Sei 079 ROOTES x 142 IIIII II IE ae og _____ 678 __ ooo _______ 006 evo afo ee MES al E 20 SER IN 141 81818 31315 4 lt lt 8151842 v I ls ef osis 005 00912 urs lees oss le 1 ili Jas USA US 9160102 gamepd 0070697 80202102 camoyoy 69181 5 A 5 S A Eu e pe s acr Eo serv 00091 x somomsy pame CER T sounansy eureueq PZOITIOT seunogoy 92012104 sougoyoy purcue4 smnomy 92012102 000 1 NE E pa enar E urn 5201 som 92012102 rmotpv game ovy C KA omes 551 a ed 7 sss s E A IE wea ll o AAC i E ET __ a MM A RT A euueg Zx TL OI AT AHA pred dene 000 sap 502
297. on with counterpart personnel on their training plan is required in order to improve the effectiveness of such training The assignment of a supervisor for each training participant is also recommended who is responsible for ensuring overall quality of the training B Issues relating to the project administration 6 Official assignment of a Liaison Officer It is advisable that ARAP assign a Liaison officer who is solely responsible for reception follow up of document application processing etc related with project administration 7 Continued effort to ensure assignment of ARAP counterpart ARAP counterparts have to travel to Achotines from their assigned areas and have been playing important roles in conducting various research activities It should be ensured that existing ARAP counterparts continue to take part in the project activities for the remaining period of the project xvi 8 Publicity of the Project The presence and significance of the Project needs to be widely disseminated to the general public of Panama and Japan It is important to organize an international regional seminar to disseminate the project results to wider audiences because tuna resources are important regional shared resources 9 Financial contribution In order to ensure financial stability of the Project it is urged that and JICA be adhered to the agreement made at JCC meetings 10 Improved management and security of e
298. op sepeJoqe o SEPIPIA e 52122452 sop ap SOSINIAL 8 eos euo Dudas FN sgue odu sosondng SOPA SO QUILLIGA OANELIEN 168 484 100102140 snuuny oogroeg op Inze ejo e unge AX snuuny r ejpueure TYE uy ojelqo 59199054 olUalsns ns wed sppeuoroe r sergsnpul sey ap op e ap uspuedep enb sougDeDnt SoloaTPHI 5 opedol uo sopeo oaur TYO s unogoy A EI 5091193 5 olafqo soup 0393A01 sosmo r 5015 qru lsos 09TTOd ap nze eeure ware 45 sop oUBIdrural opi ap A eAnonpoude1 ISOTOI ep ap soipmsqy ojpoaKolq 0107 3996 9T M994014d op oussiq zuyej er wed uorelsdo ap oysandnsaig 501 sajeuosad soen 0124014 op sodinbe uomermdg 04528 5 Sono ooaAor sodmbe soy eled sogender Ksepggumsuo sodmbg seoid 85 sgo se
299. os tambi n constituyen buenas oportunidades para que los investigadores visitantes se presenten a las otras partes 4 Planificaci n anticipada de las actividades de investigaci n Ciertos trabajos de investigaci n requieren suficiente tiempo de antelaci n para su preparaci n por ejemplo reproducci n de alimentos vivos y los investigadores de la ARAP necesitan obtener autorizaci n de viaje 30 d as antes de su partida para sus visitas al Laboratorio Achotines Estos hechos en conjunto indican la importancia de realizar una planificaci n anticipada de las actividades de investigaci n en el Proyecto Adicionalmente a fin de superar las barreras del idioma y facilitar la comunicaci n es altamente recomendable realizar una documentaci n del plan o programa de investigaci n La informaci n documenteda puede servir como una buena base para el debate y la consulta entre los investigadores de las diferentes instituciones Por lo tanto se solicita que todos los investigadores que preparen un plan o programa de investigaci n por escrito con suficiente anticipaci n a su visita al Laboratorio y que la compartan con los coordinadores de investigaci n y sus contrapartes 5 Mejoramiento de la Capacitaci n en Jap n La importancia de la planificaci n anticipada y la documentaci n de informaci n para la implementaci n de manera consultiva tambi n se aplican a la capacitaci n en Jap n La estrecha consulta con el personal contr
300. oshifumi Sawada Vernon Scholey Amal Biswas and Kenji Takii Gross energy consumption in Yellowfin tuna with embryonic development Aquaculture 2013 Nashville Tennessee USA February 22 25 2013 12 Toru Kobayashi Tomoki Honryo Yasuo Agawa Yoshifumi Sawada I OAdames OCano Scholcy V OMarguries D Naoki Yanagishita Gonad formation process of cultured yellowfin tuna The 2013 spring meeting of the Japanese Society of Fisheries Science Tokyo University of Marine Science and Technology Marzo 2013 13 ANomura S Kobayashi T Agawa Y OMargulies D OScholey V Sawada Y Yag shita Y Genetic population structure of the yellowfin tuna Thunnus albacares the North Pacific Ocean 9th Indo Pacific Fish Conference Junio 2013 Okinawa Junio 2013 14 Yasunori Ishibashi Mari Akiyama Michio Kurata Tokihiko Okada Changes the stress response and low oxygen Stress tolerance associated with the development of bluefin tuna larvae The 2013 autumn meeting of the Japanese Society of Fisheries Science Mie University Septiembre 2013 15 AMasato Awa Yasuo Agawa Tomoki Honryo Michio Kurata Tokihiko Okada and Yoshifumi Sawada Analysis of the HIF 1a gene transcripts under hypoxia and or hypercapnia in red sea bream Pagrus major early embryos Aisa Paific Aquaculture 2013 Ho Chi Minh Diciembre 2013 16 Yasunori Ishibashi Tomotaka Saida OYazmin Villarreal O Angel Guillen Yoshifumi Sawada Tomoki Honryo Ontogeni
301. p ugr og qod ey ewed A ejouanoay A ap sepeoynuspr souororpuoo Z sop sousur Y S EUOIS 1 S219 P1 5 010195 o 10d 95 Teno serune 52129052 ap ofoueur Te ejqeorjde 831533 POTI 0315 EJ 516505 sarsadsa sop sej 240 eperofaur 25190014 o13eAo14 op SOPETNsal 30 sepeseq Sosma op spgl0qelo sepipaj e sa1opeorpug Jad Spano snuuni Inze vio e urge 7 44 3S2402D91D g eeure Baye um y 0 5910945 oyuersns ns wed eamdeo anb soyauwued souppepnrO so oampur ua LVID 7 IVY V e 50911551 8 soyoaurp SouegIoyeusq ozafqo odrur Some 0109A014 opoueq 102 91quistAou op 97 20 OJ09 01H op ouesiq ZEW PLIPUOIOJH dooj q osn LJA seaun se PSDHHUSDI eoo qejse SS ddd LAA
302. periodo 1996 2000 en la tasa de xito de la captura a la formaci n de la poblaci n de reproductores saludables de YFT Con el fin de mejorar los m todos para la captura y transporte de YET se han investigado los cambios de la temperatura corporal en la columna vertebral durante el transporte Se sugiere que el estr s del YFT contin a durante varias horas despu s de la captura Se examinar n los m todos para reducir el estr s de los peces despu s de la transferencia al tanque de peces Tambi n est previsto proponer m todos nuevos y mejorados en cuanto a la transferencia de los peces a los tanques de peces Las actividades relacionadas con este indicador se completar n el pr ximo y se espera que el mejoramiento de la tasa de xito de la captura a la formaci n de la poblaci n de reproductores de YFT saludables con este indicador se complete en el transcurso del proyecto La tasa de supervivencia desde la captura hasta la manipulaci n del YFT de 1996 a 2000 fue de un 50 9 en promedio Indicador 4 5 Producci n de al menos 25 juveniles de YFT de 20 cm despu s de 3 meses en la jaula marina No se ha completado la instalaci n de la jaula marina Por lo tanto no se ha Iniciado la investigaci n relacionada con este indicador la cr a de los juveniles cn la jaula marina La instalaci n de la jaula marina est programada para febrero de 2014 La cr a de en la jaula marina se llevar a cabo a partir de la
303. quipment A variety of technical equipment and instruments are provided Recently there was a reported case of a lost item and such incident needs to be prevented from happening again In this connection it is advised that management of project equipment and instruments be strengthened 11 Consideration of countermeasures for the prolonged halting of spawning There is delay of some activities caused by the current halt in spawning It is envisaged that spawning of YFT would be resumed when sea temperature rises around April Prolonged halt in spawning would seriously affect the project achievement Therefore it is suggested that researchers concerned on this issue promptly consider viable options of countermeasures when halting of spawning continues beyond April 2014 12 Installation and management of an ocean cage Although the installation of ocean cage has been delayed in the operational stage three to four technical staff of ARAP need to be assigned for management of the cage as operation maintenance work such as feeding fish and collection of dead fish is required on the daily basis 7 Lessons Learned None at the mid term review 1 2 lt ZR PAD DS 5 fal EE H 1 1
304. r xii of persons expertize and research capacity At the beginning of the Project the dispatch of Japanese researchers was concentrated in a relatively short period of time and it was necessary to coordinate the arrangement of research materials and facilities in order to ensure smooth conduct of the research activities procured equipment is adequate and has been used effectively In the case of ocean cage there is around one year delay in procurement for the trainings Japan in most cases trainings were effective in terms of strengthening research ability of the counterpart personnel There were cases that explanation of the objectives of the training and methods of experiment were not properly done and instructions to the counterpart personnel were not made adequately 2 Inputs by ARAP Eleven 11 staff members of are assigned as counterpart personnel and it seems that number of counterpart personnel is appropriate ARAP has made substantial efforts for allocating budget however the actual amount allocated for the project activities has not reached the level that ARAP initially committed 3 Inputs by TATTC has been fulfilling its financial commitments It seems that assignment of the counterpart personnel is appropriate The issue of coordination of the research activities was also relevant to IATTC researchers because their visits to the Laboratory were made at
305. r empedu sourmdo sapejueiqure s2101297 7 Sop souaui y ASA LIA uotoe qod ey soyuawajdns LAA SEINISLIODEIEO sep WULP T 919621934 sepeogmuept seumdo seuororpuoo 2 sop ry Sopejiisy Sare SOSJ091 solu rtrIpa3o1q SOYSIP qru lsos ofaueu SASE seno sof ged mze Hoye LJA ep ep e Leone utu ep sop 121250 eured eAronpojda eI20 OIq 21 21405 204 A sjuourepenoope vzrjo1urs IS 12 25 seraume sarodso sosmoar ep ofsueu qe fPOITOB 180 019 HOTOBULIOJUL e 3p SISAIS J sonm SOSIMII SOJ OU Un 92452 sop se a1qos aur seuaureued senge sej oque e pnw e sopej nsal SO sepeseq sosmoar
306. radas ojusruepodutos tp sp seAge1eduroo sauomesnssAuy ZNI w e Jgd A LAA 9p seATe A DQISIA SEISA sep SEANEIEALIOO Jad LAA us sootuunb sootsy SO soy A op eAngmduroo SELALU se ur sns sopeuoroo os sopemideo SAMSOATIS LIA sens nur SISENE op SPAR LAA 9p rupu VIUF PI JESUSDI TeupUuooolR oF lt re Sepeugs se opus npour SILIS SOURSIO ormjneurejduroo NAY eaog 484 ALAA Saloyonpoxdal 52594 0013010150 5 um ap LAA US euotornnu OPES ap 5092209 SO 8 LAA aub s rmu rmqur sa10326 UOIIR OSIAU 5 AIP UN 14 8 5 LIA Anf TS5SIA op updos e oprpusjxo um sandsap OZ LAA 5
307. re a number of cases that sufficient time was not given before the conduct of joint research activities 4 Results Review 4 1 Relevance The relevance of the Project is considered to be high based on the facts described below 1 Needs of improving resource management of tuna species Tuna fishery is an important industry for the Central American countries including Panama Panama itself exports considerable amount of frozen and fresh tuna which constitutes an important part of forcign currency earnings However a declining trend of tuna catch is evident in the Pacific Ocean and regional organizations concerned with research and management of tuna resources such as IATTC WCPFC and ISC urged prompt conservation and management actions The objective of this Project is to accumulate and synthesize scientific knowledge and information on the reproductive biology and early life history of two tuna specics and its outputs are expected to provide a good basis for the effective management of these tuna resources 2 Relevance to the national policies concerned of Panama The Policy on the Aquatic Resources of Panama for Fisheries and Aquaculture 2010 defines fourteen basic principles which includes promotion of sustainable management of marine resources and promotion of research and development on appropriate fisheries and aquaculture technology for developing responsible fisheries and aquaculture The National Strategy for aqua
308. re not always possible especially in the pre visit planning stage However the efficiency of communication depends largely on skills ability of individual researchers and language barriers also exist As such there are several reported cases that communication became problematic On this basis it is urged that all the individuals involved in the Project exert upmost efforts to improve communication within the Project practical means of addressing this problem are suggested in the following sections 2 Official assignment of research coordinators With regard to the communication at the organization level clear definition of a communication channel often improves the situation Such channel can be easily established when a focal point is assigned at each counterpart organization and in this project the focal point can be called a Research Coordinator who acts as a centralized single channel for organization to organization communication The Research Coordinators take a lead role in organizing research activities within their organizations and discuss consult with other organization for well prepared and coordinated research work In this project such organizational level coordination is particularly important since research facilities e g experimental tanks and analytical equipment and materials e g YET cggs larvae and feed available at the Achotines Laboratory often become scarce during the peak season of research work
309. research content outline of the Early Growth and Survival and Rearing Technology Development Team SATREPS Kick Off Symposium 17th Nov 2011 Panama City 8 Biswas B K Biswas Idomoto N Kita Y Dietary alternative protein sources for juvenile Pacific bluefin tuna Thunnus orientalis Aquaculture Europe 2011 Oct 18 21 Rhodes Greece 2012 9 Y Sawada Y Agawa Y S Kim S Miyashita Tuna aquaculture and resource management Aquaculture America 2012 2nd March Las Vegas 10 Scholey OD Margulies OJ Wexler OM Santiago Reproductive biology and spawning patterns of yellowfin tuna Thunnus albacates Aquaculture America 2012 2nd March Las Vegas 11 OD Margulies Scholey OJ Wexler Santiago The use of indices of wind induced microturbulence and larval growth as indicators of pre recrui survival of yelloefin tuna Thunnus albacares Aquaculture America 2012 2nd March Las Vegas 12 OJ Wexler OD Margulies Soholey Santiago Temperature and dissolved oxygen requirements for survival of yellowfin tuna Thunnus albacares larvae Aquaculture America 2012 2nd March Las Vegas 13 OJeanne Wexler O Daniel Margulies OMaria Stein and O Vernon Scholey Collaborative research activities conducted by the IATTC s Early Life History Group during 2011 2012 Tuna Conference Lake Arrowhead Calif May 2012 Ze 14 Yasuo Agawa ATakafumi Komiya Tomoki
310. rgo existe la preocupaci n de que la paralizaci n del desove de YFT en el Laboratorio de Achotines podr afectar seriamente la ejecuci n oportuna de las actividades de investigaci n planeadas lo cual como consecuencia afectar a negativamente al nivel de logro del Prop sito del Proyecto Se prev que el aumento estacional de la temperatura marina que generalmente ocurre en el mes de abril posiblemente producir el reinicio de las actividades de desove de YFT y el Proyecto monitorear estrechamente los factores ambientales del tanque de la poblaci n de reproductores 4 3 Eficiencia Se considera que la eficiencia del Proyecto es moderadamente alta en base a los hechos que se presentan a continuaci n 1 Insumos de la parte japonesa Los investigadores de la Universidad de Kinki en general han visitado el Laboratorio Achotines dos veces por desde mayo a junio y desde octubre a noviembre parccer cl cnvio de investigadorcs de la Universidad de Kinki es apropiado t rminos del n mero de personas pericia y capacidad investigaci n Al inicio del Proyecto el env o de investigadores japoneses estaba concentrado en un 17 120 per odo de tiempo relativamente corto y se tuvo que coordinar el arreglo de materiales de investigaci n e instalaciones para garantizar una ejecuci n normal de las actividades de investigaci n Actualmente la coordinaci n del momento de las visitas de los investiga
311. rollan las tecnolog as de producci n de alevines que apoyan el estudio de ciclo vital temprano de YET Indicador 4 1 Clon BAC desarrollado para la poblaci n reproductora En cuanto al PBF se desarroll el BAC utilizando ADN extra do del test culo En cuanto al YET el BAC fue desarrollado utilizando ADN extra do del h gado Se ha logrado un desarrollo del clon BAC relacionado con este indicador Indicador 4 2 Marcadores de ADN ligados de sexo para identificar el sexo en el ciclo vital temprano de YFT Un marcador de ADN ligado al sexo del at n sido identificado s lo con los PBF cultivados de ciclo completo en la Universidad de Kinki Sin embargo un marcador de ADN identificado puede ser utilizado s lo para los PBF cultivados de ciclo completo Para poder identificar el marcador de ADN ligado al sexo del es necesario examinar la secuencia vecina del marcador ADN ligado al sexo del PBF e identificar la regi n de codificaci n y luego investigar el polimorfismo de YFT Indicador 4 3 Parasitismo de YFT descrito Se llev a cabo un examen de par sitos en los reproductores de YFT muertos en el Laboratorio Achotines No hubo infecci n parasitaria que afectara seriamente la salud de los reproductores de YFT La descripci n de parasitismo de YFT relacionada con este indicador se ha completado por lo tanto este componente se ha logrado DN 13 116 Z c Indicador 4 4 Mejoramiento de 25 al
312. rose Yasuo Agawa and Yoshifumi Sawada Effect of different dietary fatty acids on survival growth handling stress resistance and lipid metabolic related gene expression of Pacific bluefin tuna Thunnus orientalis juvenile Temminck and Schlegel Presentado a Aquaculture Research 7 Yoshifumi Sawada Tomoki Honryo Yasuo Agawa Yang Su Kim Shigeru Katayama Michio Kurata Tokihiko Okada Shigeru Miyashita Teratogenic effect of short term hypercapnia and hypoxia on red sea bream Pagrus major embryos Presentado a Journal of Fish Biology 8 Yang Su Kim ODaniel Margulies OVemon P Scholey OAmado Cano and Yoshifumi Sawada The effect of temperature and salinity on hatching rate and larval survival rate of yellowfin tuna Thunnus albacares Presentado a Aquaculture 9 Yang Su Kim OTsukasa Sasaki OMasato Awa OMaho Inomata Tomoki Honryo Yasuo Agawa Masashi Ando and Yoshifumi Sawada Effect of dietary taurine enhancement on the growth and ontogenetic development in red sea bream Pagrus major larvac Presentado a Aquaculture Research 10 Yasuo Agawa Takafumi Komiya Kouhei Tamura Tomoki Honryo Tsukasa Okada Naoki Yagishita Toru Kobayashi and Yoshifumi Sawada Identification of Male and Female Sex Linked DNA Sequence of Cultured Pacific Bluefin Tuna Thunnus orientalis Presentado a Aquaculture Research 151 1 O Amado Cano Yang Su Kim O Darys Delgado O Vernon Scholey Yoshifumi Sawada Comparative efficacy o
313. rt Chief Advisor Spawning ecology Nutrition mquireinenis Barly histor Deyclopnient of terv amp utture 18 researchers JST gt gt 1 j latte lt lt 1 1 Lougterm apet Projet Coordinator Research in Japra Tr ning in Japan Joint Research and tectnskal transferin The Achetives Laboratory man sltein Parana The Kinki The Joint Coordinating Committee JCC meetings have been held twice and in these meetings agreements were made on such agenda items as stated below Budgetary contribution to the Project from each party Selection of counterpart for training in Japan Annual work plan of the project activities A Assignment of project counterpart personnel Guidelines for use of project equipment Research sites and urget space Based on above factors it can be said that the JCC meetings were effective in discussing issues and collective decisions making In addition four sub committees steering committee research committee budget committee and public relation committee were formed for the Project and several persons of three organizations were assigned in each sub committee The members of sub committees communicate mainly through e mail however at times communication was partial or incomplete Considering the situation where the parties do not me
314. s Recently there was a reported case of a lost item and such incident needs to be prevented from happening again In this connection it is advised that management of project equipment and instruments be strengthened so as to ensure long term utilization of these items 11 Consideration of countermeasures for the prolonged halting of spawning It is envisaged that spawning of YET would be resumed when sea temperature rises around Apri 2014 and the delay of some activities caused by the current halt in spawning would be also solved For the moment measures taken on this issue are mainly close monitoring of environmental factors of the broodstock rearing tanks Considering the time bound nature of the project activities prolonged hait in spawning would seriously affect the project achievement Therefore it is suggested that researchers concerned on this issue promptly consider viable options of countermeasures when halting of spawning continues beyond April 2014 21 E 12 Installation and management of an ocean cage The installation of ocean cage has been delayed for some technical reasons Since it is an important component of the Project efforts shall be made to ensure that the installation of the cage will be completed as early as possible In the operational stage three to four technical staff needs to be assigned for management of the cage as operation maintenance work such as feeding fish and collection of dead fish 15 required on the
315. s contrato de mantenimiento de equipo y transporte de materiales El desglose detallado de los gastos aparece en el Anexo 8 3 1 2 Parte Contraparte CIAT y ARAP 1 Personal Contraparte Involucrado en las Actividades del Proyecto Al momento de la revisi n intermedia un total de diecinueve 19 investigadores y funcionarlos est n involucrados en las actividades del Proyecto de los cuales 13 personas son de la ARAP y 6 personas son de la CIAT Los investigadores est n organizados en tres grupos tem ticos seg n se describe en el siguiente cuadro Grupo Universidad de Kinki Ecolog a de desove ESA alimentaci n de tecnolog a de cr a Se inform que se s investigadores renunciaron por diversos motivos quienes fueron sustituidos SA 5 108 inmediatamente por nuevos contrapartes La lista detallada del personal contraparte aparece en el Ancxo 9 Tres investigadores de la sede de la CIAT han visitado el Laboratorio Achotines dos veces por para llevar a cabo experimentos relacionados con YFT Ellos tambi n han visitado la Universidad de Kinki una vez al a o cerca de 30 d as por visita para llevar a experimentos relacionados con 2 Equipo suministrado por la Instituci n Contraparte La ha suministrado equipos tales como computadora personal motor fuera de borda refrigerador y aparato de aire acondicionado El monto total de los equipos comprados fue d
316. s nacionales la tecnolog a los sistemas y el estado financiero 2 Marco del Proyecto 2 1 Resumen del Proyecto El marco del proyecto se defini bas ndose en las Minutas de Reuniones firmadas el 16 de septiembre de 2010 y en el R D firmado el 28 de enero de 2011 El resumen proyecto descrito en la versi n 0 1 de la PDM es el siguiente Para m s detalles v ase el Anexo 2 Meta General Se fortalecen la gesti n de recursos de dos especies de at n en las aguas paname as y el rea de jurisdicci n de CIAT 2 Prop sito del Proyecto Se obtiene y sintetiza adecuadamente el conocimiento y la informaci n sobre la biolog a reproductiva y el ciclo vital temprano de dos especies de at n los cuales son fundamentales para el manejo sostenible de dichos recursos 3 Resultados Resultado determinan las caracter sticas de desove de YFT at n de aleta amarilla at n de aleta azul del Resultado 2 Se el m todo para identificar las l neas maternas a trav s del uso de D joop mitocondrial en el an lisis lt 106 es Resultado 3 Se identifican los factores cr ticos que afectan la supervivencia de YFT y PBF en su ciclo vital temprano Resultado 4 desarrollan las tecnolog as de producci n de alevines que apoyan el estudio de ciclo vital temprano de 4 Actividades 1 1 investigaci n de la hora de desove de YFT durante un d a y l
317. sentado a Aquaculture 17 ATakayuki Ohnishi Yuichi Tsuda Wataru Sakamoto Osamu Murada Kenji Takii Post feeding changes in oxygen consumption and swimming speed in juvenile Pacific bluefin tuna Thunnus orientalis Presentado a Fisheries Science 18 Mark Polinski Sho Shirakashi Andrew Bridle Barbara Nowak Transcriptional immune response of cage cultured Pacific bluefin tuna during infection by two Cardicola blood fluke species Presentado a Fish and Shellfish Immunology 19 Gentoku Nakase Tomoki OLiliana Guerra O Diana Perz O Amado Cano ODaniel Margulies O Vernon Scholey and Yoshifumi Sawada Addition of Nannochloropsis oculata in pre rearing water improves survivals of Yellowfin tuna Thunnus albacares larvae Presentado a Aquaculture Science 2 Otras publicaciones 1 Yoshifumi Sawada An expertize concerning on the artificial hatching and larval rearing of the Pacific bluefin tuna Proceedings of the Symposium of Taiwan and Japan Aquaculture Engineering on the Envirorunent Balance Management and Technology 312 336 2010 2 Kenji Takii Yesterday today tomorrow of bluefin tuna nutrition and feed research perspectives to inexpensive formula feed for larvae Aquanet April 2011 68 71 lt 152 3 Yoshifumi Sawada Toward the stable development of bluefin tuna artificial seedling production the branding of the product intellectual property of technology Suisankai March 2013 1538
318. sis En cuanto a PBF se recogieron y analizaron muestras de 500 individuos o ejemplares de PBF que se obtuvieron del mercado de pescado en Jap n Tras una serie de experimentos en este proyecto se identificaron los Primers para detectar el polimorfismo de D loop mitocondrial y se ha logrado este componente Indicador 2 2 L neas maternas Independientes de YFT identificadas En cuanto a YFT se recolectaron muestras de 25 individuos de YFT capturados en Jap n y 77 individuos de YFT desembarcados en Panam Los resultados del an lisis indicaron que la diversidad gen tica era amplia pero no se encontraron poblaciones dominantes Sin embargo con el fin de verificar estos resultados se emplear n otras metodolog as 114 Indicador 3 2 Desarrollo externo e interno morfol gico de larvas y juveniles descrito para YFT y PBF 3 3 3 Resultado 3 Se identifican los factores cr ticos que afectan la supervivencia de YFT y PBF su ciclo vital temprano Indicador 3 1 La velocidad del desarrollo y el proceso de embriog nesis de YFT y PBF descritos en relaci n con los factores f sicos y qu micos Se complet la observaci n microsc pica e histol gica del proceso de embriog nesis de YFT y su an lisis est en marcha En cuanto a los efectos de salinidad y temperatura del agua en la velocidad de desarrollo tasa de eclosi n tasa de supervivencia y la investigaci n de los efectos sobre el progre
319. so de la morfog nesis tasa de eclosi n tasa de larvas norinales y cantidad diaria de muerte de peces se llevaron a cabo La comparaci n sobre los datos sobre PBF est en marcha En resumen los experimentos y an lisis relacionados con este indicador se han completado por lo tanto se ha logrado este componente Se concluy la investigaci n del desarrollo externo e interno morfol gico de larvas de En cuanto al YFT se ha completado la recolecci n de muestras de morfolog a externa La recolecci n de muestras de morfolog a externa de larvas y de las morfolog as externas internas de juveniles y de peces j venes de YFT y los an lisis se Hevar n a cabo en el futuro Indicador 3 3 Similitudes y diferencias identificadas en las caracter sticas de visi n y reacci n de larvas a la informaci n de YFT y PBF a la luz la ecolog a de la alimentaci n comportamiento crecimiento y supervivencia de YFT y PBF el valor nutricional de los tipos de alimentos artificiales y naturales de YFT y PBF 1 Las caracter sticas visuales y reacci n a la luz de las larvas y juveniles Se han llevado a cabo estudios comparativos sobre la cantidad de alimentaci n crecimiento y tasa de supervivencia de las larvas y juveniles de cr a en condiciones diferentes utilizando luces LED que diferentes longitudes de onda Como se ha mencionado anteriormente dado que una caracter stica visual de las larvas de YFT y PBF se confirm la s
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321. ssary As for the trainings in Japan in most eases trainings were cffective in terms of strengthening research ability of the counterpart personne in total 13 persons participated in There were cases that explanation of thc objectives of the training and methods of experiment were not properly done and instructions to the counterpart personnel were not made adequately e 17 2 Inputs Eleven 11 staff members of are assigned as counterpart personnel for the Project at present t seems that the number of counterpart personnel is appropriate ARAP allocated budget for the project activities including travel expenses fuels and procurement of equipinent ARAP has made substantial efforts for allocating budget however the actual amount allocated for the project activities has not reached the level that ARAP initially committed 3 inputs by terms of financial contribution to the Project has been fulfilling its commitments With regard to assignment of the counterpart personnel the director and other two researchers of the Achotines Laboratory have participated in the project activities and three researchers of IATTC headquarters in California USA have visited to the Achotines Laboratory and carried out their experiments In addition three researchers of headquarters have visited to the Kinki University in the spawning season of PBF and carried out the experiments relat
322. stocks are kept and spawning is observed in captivity At the Achotines Laboratory various reseatch activities are conducted jn close collaboration with the Aquatic Resources Authority of Panama hereinafter referred to as ARAP Based on the background described above the government of Panama made a request to the Government of Japan to catryout scientific technical cooperation on Comparative studies of the reproductive biology and early life history of two tuna species Yeliowfin and Pacific Bluefin tuna to ensure the snstainable use of these resources herein after referred to as the Project The Project was commenced in April 2011 as a 5 year project 1 2 Objectives of the Mid term Review 1 To confirm the progress and achievements of the Project based on the Project Design Matrix PDM and the Plan of Operations PO and identify the promoting inhibiting factors to them 2 To analyze and evaluate the Project from the viewpoints of five 5 evaluation criteria i e Relevance Effectiveness Efficiency Impact and Sustainability and 3 To make recommendations on actions to be taken during the latter half of the Project 1 3 Meniber of the Joint Review Team The Project was reviewed by Joint Mid term Review Team hereinafter referred to as the Team composed of persons of the Japanese side and the counterparts Panama The Team was composed of five 5 members from the Japanesc side and three 3 members from th
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324. t have been achieved as planned It also examines whether these benefits have been brought about as a result of the Project 2 LE 3 Efficiency Efficiency refers to the productivity of the implementation process examines whether the inputs of the Project have been efficiently converted into outputs 4 Impact Impact refers to direct and indirect positive and negative impacts caused by the implementation of the Project including the extent to which the overall goal has been attained 5 Sustainability Sustainability refers to the extent to which the Project can be further developed by the authorities concerned of Panama and the extent to which the benefits generated by the Project can be sustained under national policies technology systems and financial state 2 Outline of the Project 2 1 Summary of the Project The framework of the project was defined on basis of the Minutes of Meeting signed on September 16 2010 and the R D signed on January 28 2011 The project summary described in PDM version 0 1 is as follows For more details see Annex 2 1 Overall Goal Resource management of two tuna species in Panamanian waters and IATTC jurisdiction area 15 strengthened 2 Project Purpose Scientific knowledge and information on the reproductive biology and early life history of two tuna species which are fundamental for the sustainable use of these resources are adequately obtained and synthesized 3 Outpu
325. thesis of scientific and_ The information to be Purpose information applicable to technical information synthesized is not only resource management of tuna applicable to resource biological species which is disseminated management of tuna species by means of which is disseminated by publications means of website publications regional seminars workshops website regional seminars workshop 9 Project publications Achotines Laboratory official website Seminar workshop proceedings Synthesis report YFT juveniles of 20 cm produced after extended rearing period Project publications Achotines Laboratory official website Seminar workshop proceedings Inclusion of additional means of verification Means of Verification for Project Purpose At least 25 YFT juveniles of 20 em produced after 3 month period in ocean cage Indicator for Output 4 Technical correction 4 Assignment of the Research Coordinator Based on the recommendations made by the Team ARAP IATTC and Kinki University nominated the Research Coordinators as listed below ARAP Mr Amado Cano Ms Yazmin Villarreal Ms Thelma Quintero Mr Vernon Scholey Daniel Margulies Kinki University Dr Yasuo Agawa Dr Amal Kumar Biswas f 52 VR JCC approved the assignment of the Research Coordinators as listed above
326. ts Output 1 Spawning characteristics of YFT Yellowfin tuna and PBF Pacific Bluefin tuna are determined Output 2 method to identify maternal line of YFT is established by using mitochondria D loop for analysis 3 Critical factors that affect survival of YFT and PBF in their early life history are identified Output 4 Fingerfing production technologies that support early life history study of YFT are developed 4 Activities 1 1 Investigation of YFT spawning time in a day and spawning seasons 1 2 Investigation of environmental factors affecting the YFT spawning 1 3 Investigation of the effect of nutritional status on the spawning of YFT 1 4 Development of a simple but thorough method to examine the physiological status of brood fish larvae and juveniles of YFT and PBF cDNA library and microarray development of visceral organs including gonads 2 1 Examination of mitochondria D loop as a method to identify maternal line of YET 2 2 Verification of the method by analyzing selected wild caught YFT samples to examine their maternal lines 3 1 Comparative investigation of early development and the effects of physical and chemical factors on YFT and PBF 3 2 Comparative investigation of the vision characteristics and the larval response to light information in YFT and PBF 3 3 Comparative investigation of feeding ecology behavior growth and survival of YFT and PBF in early life stages 3 4 Comparative investigation of
327. umi um 225085 O soapy meted oyoos tc Jawy Aonesommerodary 000 219 2 waTshS RAE amma mev ea t a wesson mi soy rua s L EEE CE o mw 52011106 yureued 211029760 1091515029 205527101 em s ae sine ee e pp ON EE EE Q wes TZ FONERA a U U U A O papa EUR El LUE 344 mos SC STZI X3d 138 r 815267 02601105 SPuyoyoy 0011401 5 51401142 Smhomy yeueg 0059260 5 lt 5 50801102 0070667 5080110 00067711 5 TAE oi O MAA Dororstnhpe ra 1edrouug ugror nm dsq po Le vra iztoc FCOICIOZ Suunouov gumueq veoizioe ssmegpy panud p 5 m SNA a sos a wear ae EEE ZDKZEOZ seunoqpv gummupd _ gz mam 1946 Sra
328. unicaci n era parcial o incompleta Teniendo en cuenta la situaci n en la que las partes no se re nen de manera regular la funcionalidad de estos subcomit s puede 15 118 Ze ser limitada por lo que se necesitan medidas complementarias para resolver este problema 3 5 2 Proceso de Implementaci n del Proyecto 1 Rotaci n de personal contraparte de la ARAP Al inicio del Proyecto la ha hecho sus m ximos esfuerzos para asignar el personal contraparte en todos los campos de investigaci n Sin embargo 6 funcionarios contrapartes renunciaron a sus empleos pesar de que estas posiciones vacantes de las contrapartes fueron reemplazadas r pidamente fue necesario reiniciar el desarrollo de las capacidades de estos investigadores 2 Comunicaci n entre los investigadores involucrados en el Proyecto Se ha informado de que la comunicaci n entre los investigadores Japoneses y el personal contraparte no ha sido tan fluida en algunas ocas ones Se indic que dicha comunicaci n deficiente podr a haber afectado a la ejecuci n eficiente de las actividades del Proyecto 3 Planificaci n anticipada de los trabajos de investigaci n en el Laboratorio Achotines En el Laboratorio Achotines se requiere que el plan de investigaci n se presente al administrador del Laboratorio con suficiente tiempo antes de la llegada de los investigadores con el fin de garantizar la utilizaci n coordinada de las instalaciones y los equipos
329. uogeqnsig epa mdr ser jos Lp sb p je k Ep e SIS TEUe USW 10 stadem Utoyosg 203 Goss OZ d 920 900 2001 pe me NEN SYSAjeuP Y SISATEUE 2291 207 AI E PA ES PON ap c _______ x wmemywmws 005 5204645 serie 91 9 08 957 950 LINE Qv amp sn 36 X norisatosdS pue Japoy u m mdmb jo aure smamo PE E vt N dozosld ss 3moury 1 3dKLpIQS SSILKA t 71 MM a d t d 16 ossis 021 1102 sowy 01 er veder weur TURIRA emen 15995 annes 0001 42 Poeg dh kao ats et emm Aa Mc A sue T 4 enby J UB T 8 18 L L LL L L L L B B 61011107 ssunorpv 1222 566525 tr 4 71102960 12525 sds amsseudi LE DV 02601102 Saunoya
330. uogjeziuva1 Aq suomdmby DT jo ssamosoyy 6 oyo os 000000cc 56727592 00616 OLZ 19301 mol proz etoz 100 210 102 110 pue avuv JBUOTSIAOI dy A oeg rr x hs ww me AA _________________ ol 00001 ooo ner he me 107 5105 1107 sesusdxz eoo soupoypy pue 00009 000s NO s cca me 40285 Q SUOSESS 1930144 pue 901105 UII97890S 91369 9092111 8006225 2 suosEos pue 119 9 gsp 9z 91780241 1 00064 ww memo w ESM sasundxg Aq 1502 E20T T Aq p93890 Y 300 1 20 1 TT OLLVI 3oje10qe paresore 1500 8907 0 DENEZ E oL 1230 1 1 sosuedxo Janz ISsatT sosusdx or 5005295 2 suosg s 193014 pue pay Annex 12 List of Publications 1 Publications in academic journals 1 Yoshiki Tsutsumi Taro Matsumoto Tomoki Yasuo Agawa Yoshifumi Sawada and Yasunori Ishibashi Effects of light wavelen
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332. y t cnicas adquiridas por el Proyecto muy aplicables al cultivo de otras especies marinas Estos incluyen reas t cnicas de producci n de semillas manejo de criaderos cultivo de alevines desarrollo de la alimentaci n manejo de jaula marina y an lisis gen tico Se espera que el personal contraparte trabaja en estos reas contribuya al futuro desarrollo y promoci n de la acuicultura en Panam 4 5 Sostenibilidad La Sostenibilidad del Proyecto probablemente ser alta con base en los hechos descritos a continuaci n 1 Aspecto Pol tico Como se mencion en la Secci n 4 1 el logro del objetivo del Proyecto contribuir al manejo sostenible de los recursos marinos y al desarrollo de la acuicultura a mediano y largo plazo El Gobierno de Panam considera estas reas como temas importantes Por lo tanto se garantizar la sostenibilidad pol tica del Proyecto 2 Aspecto Organizacional y T cnico La ARAP y la CIAT han llevado a cabo investigaciones conjuntas desde 1985 en el Laboratorio Achotines y la CIAT es responsable de la conservaci n y manejo del at n y otros recursos marinos en el Oc ano Pac fico Oriental Por lo tanto es muy probable que esta estructura de investigaci n conjunta contin e a mediano y largo plazo Para asegurar la sostenibilidad t cnica es fundamental que el personal contraparte que ha adquirido conocimientos y tecnolog as relacionadas contin en sus trabajos en su respectiva instituci n
333. y wueueg 0010701 5 wo E a KCN KH TL lz 0L 89 19 BB saeeY E DE CTO Sun 01 90110696852 4 354 1202910 1 TRA ANS 1905 0 0 O98 Op6S 3C0AI BIEP 20 os 00 35 somqeq Te TITLE 2021102 4 006800 X Emri ms ss amp nyemdda uogas AA DIR 35 a veder sc waue 690 nur EY pue yapo Sou m JNS 451814 tt yo auey yuotuarmsotd 8 3 5 6 lt PAN 3 5 E e ISEXISI ZV vasaa es 10 a ex foot x 1 TUORO ANOS mms 992 d ANOS 556 mojaego Joy 13526201 DIPIA ance PZOLTIOT PZOIZIOZ sounoqow eureueg PZOIZIOZ ssumoqoy u P201ZIO0Z seuncuov 76012102 Sumnyoy gnrenpd 72012105 720 12100 Senoyoy lt E weder uode 011 504 i WOULD 8 E min O mr
334. yashi JICA Panam Asesora de Cooperaci n T cnica 21 CarlosZambrano JICA Panam Asesor de Cooperaci n T cnica 22 Onodera JICA Panam Relaciones P blicas 23 Esperanza Paniza Embajada del Jap n Representante de la Embajada de Jap n 1 5 Suguru Kubo JICA Planificador de la Evaluacion 1 17 yes 100 INFORME DE REVISI N INTERMEDIA CONJUNTA SOBRE LA COOPERACI N T CNICA JAPONESA SATREPS PARA ESTUDIOS COMPARATIVOS DE LA BIOLOG A REPRODUCTIVA Y DEL CICLO VITAL TEMPRANO DE DOS ESPECIES DE AT N ALETA AMARILLA Y ALETA AZUL DEL PAC FICO EL MANEJO SOSTENIBLE DE ESTOS RECURSOS Ciudad de Panam 26 de noviembre de 2013 po d Licdo Edison Cedefio Sr Shun FS giyama L der L der Equipo Japon s de la Revisi n Intermedia Equipo de Revisi n Intermedia de las Instituciones Agencia de Cooperaci n Internacional del Contrapartes Jap n A Autoridad de Recursos Acu ticos de 117 EL 101 07 Tabla of Contenido 1 Introducci n 11 Antecedentes del Proyecto 1 2 Objetivos de la Revisi n Intermedia 1 3 Miembros del Equipo de Revisi n Conjunta 1 4 Programa la Revisi n Intermedia 1 5 Metodolog a de la Revisi n Intermedia 2 Marco del Proyecto 2 1 Resumen del Proyecto 3 Logros del Proyecto 3 1 Insumos 3 2 Avance de las Actividades Programadas 3 3 Resultados 3 4 Prop sito de Proyecto 3 5 Estructur
335. zen AISIAN 14013 souas amp ONES vuregenge amp 591394514 uongls AISIaAIUIT MU Aroreroqe T S9LOYSIA uone S ISA 141 102 E 21482 105125 OC 25029591004 ISA Qc e uaasaronga jeisds aw O d 35029521014 Jalam e EE D Araun yury aimnousy jo Amau Atoperoqe 5 190514 oO AAD PUTA Jo ASIAM vnreuers Asrmun pan Amory ECOCIOZ worms 02805 0 06 001 285 PIT OQ oxf es eio Araun AUN sa mymarmv jo juouidinbg jo Ec 8676 192 72590 81 PL EPS 96 0009581 91 67675 00700297

パナマ共和国（科学技術）資源の持続的利用に向けたマグロ類 2 種の

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パナマ共和国 （科学技術） 資源の持続的利用に向けたマグロ類 2 種の

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パナマ共和国（科学技術）資源の持続的利用に向けたマグロ類 2 種の