MY4-FITC
Contents
1. CYTO STAT COULTER CLONE MSIg2b FITC2. CD14 MY4 No Wash 2 nn 3 nn 4 nr 5 Ns MY4 CD14 CD14 859 5 10 MY4 MY4 CD14 Mo2 HL 60 MY4 5
3. 10uxL MSlg2b FITC 10L 10 TQ Prep Q Prep 1 0 5mL 11 2 2 2 8C 24 1
4. 3 Mslg2b FITC 6603034 50 0 5mL EDTA 1 100L 1 1 lt 2mL 3 10 x 10 A mm 20 25 C 6
5. a gt 10x10 mm 10 20 x 10 2 20 30 x 10 3 30 40 x 10 5 40 60 x 10 6 60 100 x 10 10 100 200 x10 20 b lt 3 x 10 IE mm 1 25 C 500 x g 5 2 COR FAT EE LH 700 5 3 10 10 mm 3 4 Q Prep 12mm x 75mm 100L
6. 6604110 2009 11 2007 2 21700AMY00059000 MY4 FITC 1 2 3 4 CD14 MY4 0 11 g FITC ERBE 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN 0 1 CD14 55kD CD14
7. GPI LPS LBP 1 859 MY4 MY4 CD14 Mo2 RARA ICEX 322A 1 MY4 BALB cJ VUA RR HDA e Z 77 ABRE P3 NS1 1 AG4 1 lg lg2b H RUK L FITC Fluorescein Isothyocyanate FITC 5 10 468 509nm 504 541nm CD14
8. PBS 3mL 400 450 xg 5 PBS 10L 1 7546999 300 TQ Prep Multi Q Prep 7546946 100 Q Prep LIF LYIREVAT A a A b B co C 6603152 2 PBS 1 4 PBS 6603369 1 500mL pH 7 2 0 2
9. 488nm 525 504 lt 541 nm 1 2 3 EDTA 24 90 10 000 mm 10 000 mm
10. GPI PNH CD14
11. HL 60 MY4 MY4 CFU CD14 y 1 04x 0 87 r 0 97 n 54 40 _ 30 amp of 20 EN K 10 0 0 10 20 30 40 96 1 2 3 4
12. 2 FS 90 SS 3 FS 3 FITC EH Log 1 1 2 3 4 5 2 6 2 4 Neg Cont Mslg2b FITC EPICS XL SAME Neg Cont CS MY4 FITC gg ss 1 FLOW COUNT 7547053 2 CD14 mm
13. mm x CD14 9 10 MSlg2b FITC 1 29 2 1 CD14 SD 7 6 2 2 n 20 CD14 CD14 CD14 CD14
14. 5 6 7 MY4 FITC 2 lt 8 18 1 2 8 C 2 MY4 FITC 6604110 1 Bernard A Boumsell L Dausset J Milstein C Schlossman SF Leukocyte Typing 1984 Berlin Springer Verlag 108 407 2 McMichael AJ Leukocyte Typing Ill White Cell Differentiation Antigens 1987 Oxford Oxford University Press 586 589 3 Todd RF Griffin JD Ritz BU Nadler LM Abrams T Schlossman SF 1981 Expression of normal monocyte macropfage differentiation antigens on HL60 promyelocytes undergoing differentiation induced
15. by leukocyte conditioned medium of 50 0 5mL 3 4 phorbol diester Leukemia Res 5 491 495 4 Griffin J D Ritz J Nadler L M and Schlossman S F 1981 Expression of myeloid differentiation antigens on normal and malignant myeloid cells J Clin Invest 68 932 941 5 Goyert SM Ferrero E Rettig WJ Yenamandra AK Obata F Le Beau MM 1988 The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors Science 239 497 500 6 Pedron T Girard R Chaby R 1995 Variation of LPS binding capacity epitope expression and shedding of membrane bound CD14 during differentiation of human monocytes J Immunol 155 1460 1471 7 Kawakami Z Ninomiya H Tomiyama J Abe 1990 Deficiency of glycosyl phosphatidylinocitol anchored proteins on paroxysmal nocturnal haemoglobinuria PNH neutrophils and monocytes heterogeneous deficiency of decay accelerating factor DAF and CD16 on PNH neutrophils Brit J Haematol 74 508 513 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC econ COULTER 6604110 0911 4 4
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