＜サブセル 96/192 シリーズ＞ 取扱説明書 - Bio-Rad
Contents
1.2. H r ad or H 000000000
3. r2 0000000000000 1 3 000000 000 000000000 2 1 2 2 000000000 2 3 0000000000 2 4 0 0000000000000 2 5 0000 26 00000000 OOO 0000000000000 31 0000000000 3 2 DNAD 33 DO 3 4 0 000 00 0 00000000 000 0000000000 4 1 00000000000 4 2 000000000000 43 00000 4 4 RNase 0 000 00000000000 000 00000000000 000 61 0000000 90000 1920000 1 00000000 11 0000 00000000000000000000000000000000000000 DNA 0 PCR 00000 00000 000000000000000000000 DNA 0000000000 0000000000000 000 020 bp 20 0000000000000000 00000 961 12 000000000000000 000000000000 000000 00000000000000000000000000000 000000 0 00000000000000000000000000000000 04800000 3 00 0000000 510 00 000 00 00 100000000000000 200000000000000 9600000000000 2000000000000 000000000 19200 040
4. x G x G 60 C G 43 00000 0000 SQO000000000 000000 000 the banana plug electrode wre 0 00 0000 0000 00000000 0000000 0000000000 1 000000 0000000000000 the banana plug electrode wire 000 00000000000000000000000000000 000000000000000000000000000 2 00000000000000000000000000000000000000 000 3 000 0 000 0000 00 000000 16 4 0000000000000000000000000 00 0000000000 000 Figure 4 1 Replacement of banana plug electrode wire assembly 4 4 0 000000000000000000000100 3000001049000 9 00 dO 0000000 ooo A 0 00 000 0 0 gt RNase
5. 000 EN61010 EN61010 T 0000
6. 0 2 5 4 0000 0 0000000 inn U D e 254 0 302 80 360 90 6 00000000000 Bio Rad Standard Polaroid Gel Documentation System Bio Rad Gel Doc system 00000000000 000 00 0 0000 0000 0080 000 0000 00000 000 00 00 000000 000 0008 000 0000 00 000 0000000 00000 00000 00000 13 2 7 00000000000 000010000000000000000000000000 00000000 3 0000000000000 3 1 0000000000 EDTA 0000000 000 0 0 0000000000000 01000 000000000 10x 0000 050x TAE 0000 000000000 C 00000000 MOPS 3 N morpholino propanesulfonic OOH OO 0000000000 1x 000 00 D 89mM Tris pH7 6 897M 2 EDTA 10x TBE 100000000 Tris FW 21 1089100 00 0 09 0 60 00000000000 FW 618 559 05 80 40m0 0 000 000 10000000000 00000000 1 900 0 D 40mM Tris pH7 6 20mM 1mM EDTA 50x TAE 100000000 Tris besq FW lt 1212422
7. 000 RNase 17 5 01000000000 00000 000 00000 10 00000 0000000 0000030 45 0 00000000 b 0000000000000 000 b 00000 0000000 000000000 00000 0000000000000 0000 0000000000 0000 000 000000000 0000000000000000 000 0 00 0000 000 0000 0000 0000 000000000000 b 00 0000000 00 00000 b 00 00000 000 00000 0000000000000 0000000000000 00000000000 5 000000000 b 0000 00000 000000000000 00000 0000000000 0 0000000000000 00000 00000 00000 0000 00000000 5 000000000 00000 5 00000000000 000 00000000000000 0000000000000 00000 0000000000000 000 000000000 0000000 0000000 0000000000000 00 00000 0000 00000 0000000 00000 0000000 0000000 5 0000000000000 00 0000 b 0000 01 0 00000000 0000000000000 0000000 0000000000000000 00000 000 00000000 00 00 00000000 e 000000 000000000000000000 0000000 00000000 0000 0000000 00000 00000000000000
8. E 3 3 000000 00000000 00 00 UI 000000 D DD 00000000000 DEPC 9 0 RNA 3 0u 5 5001 123M 00000000000 37M 4 54 00000000000 50 125g 3 4 I 000 00 0 00000000 10x 000 00 0 0000000000000 1 0000 0 500 000000 00 25 00 00000 000 00 250 0000 00000000000000000 3 5 1m0 0 1075 000 JBio Rad 0 000000000000000 O00 1019 10 0000000000000000000000000000000 00000 15 4 41 00000000000 1 0000000 610000000000000000000000 00000000 000000000000000000000000000 0000000000006 00000000000 0 00000000000000000000000000000 2 00000009490 0000000 0 0000000 3 00000000000000000000000000 95900 000000000 00000009400 0 000000 00000000000 4 2 0000000000000 0 00000000000000000000 000000 000000000000 0 0000 30 0000000000 0000000 000 000000000000000000000000000000000000 00000000000000000000000000000000000 0000000 9000000000000 UL DU D UO D U 000000000 0 000 000000 0000000000000 000000 0000 0000000 00000000000000
9. 9019200000 170 4500 to 170 45111 000000000000000000 00000 0000000000 00000000 0000000 000000000 0000000000000 0000000 0 00000000000000000000000 0 0000000000000000 00000000 000000 0000000000 0000000 00000000 0000000000 00000 0000 e e e 00000 0000000000 e 0000000000000000000000000000 e 00000000000000000000000000000 000000000000000 e 000000000000000 e 0000000 e 0400 e 0008 D W 310 m50 400 D e 00000 100 506 0 000000000000000 00000000 e _0000000000000000000000000000 00000000 00000000000
10. 00 600mg n 000000000 571211 0 5M EDTA pH 800 100m0 0 D DO 0 001400000000000 GLO DO 000 1 MOPS 0000 ORNA Gel TT 0 02M MOPS 3 rrorpholino propanesulfonic acidl pH 7 00 8 sodium acetate 1 EDTA JH 8 0 5 MOPSO 0000 110000000 MOPS 206000 00 600m 0 0000000 03M sodium acetate 0 T 05M 8 0 DEPC 0 0 10900 0000 001000000 00000000000000 14 3 2 00000 20000000000 RNAV 00000000000 1000000 000000 100700 000000 1 60 0000 62m 5 MOPS 0 0000 0000 2020 1x0 12 3 037 5961 0 0 000 18 22
11. DNA 000000000 0 0 0000000 000000000000000000 2 0000000000000 3 00000 4 0 0000000000000000 4 000000000000000000000000000000000 006 00000 000000000 00000000000000000000000 00000000 5 0000000000 00 00000 00 000 0000 0000000 0 0000 1 1 0000000000 000000 6 0000000000 7 000000000000000000000000000000000000 0000000000000000000000000000000 8 0000000000000 0000000000 0000 00000000000 230 00000 6 700000000000000000000000000 240 00000 6 0000000000000000000000000 0 000000 00 000000 000000 00002000000 00000000 0900 00000000 00000 00 0000000000 D Da 00 00 0200000 23000000000 Cell Type 00 0000000000000000 0000000 96 200V 5 15cm hr 96 200 6 20cm hr 005 01 0 000000 0 Molecular Biology Certified Agarose 1 0000 0 0 50x 0 00000 0000000 0000000 D 24 0000 000 000000 00 000000 00000 00000000000 05 15 4 5Kb 400 500 bp 20 30 750 bp 100bp 40 50 125 tp 25bp Anpiisize 0 00 000000000 9 00000000000000000000000 00000000000000 000000157 0 0 0000 0000
12. 210 22 0000000000000 0000000000 000010 000000000001 0000000 19090 0 0000000 1900 DU 21 0000 DNA 0 0 50 1 30 0 75 0 8 12 1 00 0 5 10 125 04 7 150 02 3 2 5 0 01 05 Bio AmplSize agarose 0 2 20000 000000 0 5 01 07 5cm l0cm 000 25x 10 0 98 1251 185m 250m 25x 15 0 1920 1851 280m 375m 000 25x 10cm 125111 185m 250m 25x 15cm 185m 280m 375m 25x 20cm 250m 375m 500m 25x 25cm 310m 4651 625m 2 00000000 02509 0000000000000000000000000 U 1x0 000000 00 00000 00000000000000000000000 0000000 0000000000000000000 00000000000 0 0000000000000000 00 60000000000000000 0000000000000 00000000 000000 000 0000000 000000000 0000 0000000000 0000000000000000000000 0000000600 000000000 000 00000 gnari d 000004500 0000000000 000000 DOO 000 00000 0000000 0000000000000 0000000 00000 00 000 00 00000000 00000000 4 00000000000 00000000000 000 00000000000 00000000 0000 000000000000000000000000000000000000000 000000000000000000000000000000000000000 0000000000 000 00 0000000000000 0000000000 0 00000000000000000000000000000000000000 000000000 5 00000000000000000000000000000000 0000 OOOO 0000000000000 2 2 HB D 0000000 000000000000000000 000 960 19210000000000000000000000000000000 000 000000000 0000 0000 000 0000000000000 0
13. 60000 00000000000 0000000000000 0 0000000000000000000000000000000000 UVTPQOOOOO 000000 000000 000 0000 0 4 00000 0 0000 000000 24 0000000000000 00000000000000000000000000000000000000 0002 0000000000 50 20 volts 0 0000000000 0000 000000000 00 0000000000029 00000000000000000 00000 00 000000000000000000000000000000000 00170 45311 00000000 000 000 0000 000 0000000000 D uuu 1 00000 000000 0000000000 2 0000000000 00000 000 00 000 00 0000 0000000000000 0000000 10 Tubing Figure 2 2 Connecting the recirculation ports and tubing 3 4 0000000000000000000000000000000000000000 00000000000 5 000000 300 50071 ming I 0000000000 00000000000000 0000000006000 000000000000000000000000000 0000000000000 000000000000000000000000000 0000000000000000000000000000000000000000 0000 0000000000000 2 5 0000 000000000000000000000000000000000000000 0000000000000 00000000000000000000000000 0 0000 OOO 0 EDTAJ 00000000000 0 00000 0000 00000000 00000000000 00000000 1 00000000000 00 00000000000
14. 0 00006000 m uuu B P P UUDUDU Electrical 2 Cables Safety Lid Recirculation Port Plugs Electri Leac UVTP Gel Tray Fluorescent Ruler Comb Holder Slot Gel Casting anana Plug Electrode Ae a P re Assemb S gt A 110000 000 960000 1920000 Gel Caster Leveling Bubble Leveling Feet Comb and Comb Holder UVTP Gel Tray Movable Wall Fixed Wall D 1 2 0000 000 960000 1920 00000000000 1 4 00 0000000 96 000000 Wx Dx Hj 290cmx 295cmx 9 0cm 00000000000 20L 000000000 25x 10cm 000000000 25x 10cm 25x 15cm 0000000 192 Dx Hg 29 0cmx 39 5cmx 9 0cm 00000000000 3 0L 25x 15cm 000000000 25x 10cm 25x 15cm 25x 20cm 25x 25cm 000 0000 0000 000000 00000000 0000000 44 em 025rm 000 0000 00000 00000 000000 000 0000 0000000 00000000 0000000000 0000 0000000 00064 0000 2 000000000 000 0000000000 300000000000 21 0000000000000000 OOM 210000 1 0
15. 0 0000000000 00000 000 0000 000 96000 10 015 0 0000000000000 192 0 0 0 10115120125 nn 00000000000 991000 192000000 0000000000000000 0 Genomic DNAQ 0 000000000000000 960 1920000000000000000000000000000 00000000000000000000000000000000000060000 00000000 000000 000000 0 00 000 000 000 000 00000 000000000000000000000000000000000000000000 2400000000000 00000 1 2 0000000000000 00000000000000000000000000000000000000000 00000000000000000000 0000 00 00 0000000000000 0 00000000000000000000000000000000000000 9000 0 19200000000000000 00000000 00000000000 000000 0000 200 D ul 70 Watts 000000000 50 401 40 C ut i 000 1010 1000 DO D 0 DD 0000 0 00000000000000000000000000000000000000 00000000000 00000000 00000000 1010 10 0 0 00000 e 153 000000 0000000 96000000 192 000000 00000000 0000 DU 0000000000600 00000000 9 0000 000 DU B 0510 00 0157770 0 000000
16. 0 0000000000000 0000000000000000000000 0000000 0000000 0000 0000000000 000000010 2170 0000000000000000 90000000000 0000 90000000 00000000 1100 0000000 00 0000000 0000000000 000000000 00000000000 0000 5 0000000000 5 0000000000 00000 00 000 000 00000 00 000000 000 000000 000 0000000000000000 000000000000000 0000000 0000000 0000000 0000000 00000000 000000 0000000000 00 0900000000000 00000000 0000000000000 00 18 6 00000000000 000 61 0000000 90000 1920000 0000000 961000 170 4500 A 9 25x 00 000000000 0 0 000 0 00 00000670 000000000 20 96 1920 25x OO HD 0900 0 00 00 0000 20 510 00 15 260 1 599 000 9 192100 00000 00000000000000 170 4501 0000 91000 25 15 00 000000000 170 4502 0000 960000 25 10 000 0 170 4503 000060000 25 15 0 0000000 1920000 170 4504 21921000 25 10100 000000000 192110 000000 0 000067 000000000 20 0000 96 1920 25x 0 00 00 01920 U 00000000 21 610 145m0 0 281 00 15502010 000 96 1923800000
17. 00 uvrP 01000 00000000000 000000000 0000000000000000000000000000 0000000000000000000 1 0000000000 50000000000 2 00000000 50000000000000000000000000000000 00000000000000000000000 3 0 00000000 00000000000 uvrP 00000000000000 1 2117000 000000000000000000000 1000000000000 000000000000000000000000 000000000000000000000 23 0000000000 960000 1920 40000000000000000000 00000000000000000 000 000000 0000000000000000 c 00000000 0000000000000000 b 0000 0000000000000000 000000000000000000 0 0000000000000000000000000 0 0000000000000000000000000000000000000 0 00000000 0000000000 000000000 0000000000 4 50 60 0000000 00 000000 000000000 00 000000000 0 000000 000000000 00000 0000000600000 5 000 30 60000 00000000000 000000 0000000 e 0000000000000000000000000000000000 00000000000 7 000000 4 0 00000000000 00 00 00 00000000 00000000000 300000000000 WNP 000 000000 1 00000000000000000000000000 2 00000 vP 00000000000 9600000 025 10mg 000 00000000 19200000 025 1500 00000000 3 0000000000000000000 000000000000000 000000000000000000000000000000000000 00000000000000000 4 0 00000
18. 00 00000000000000 170 4505 A OOOO 1920000 25 1 5 170 4506 00001920000 25 20 00 000000000 170 4507 A 1920000 25 25 00 000000000 170 4508 0000 1920000 25 10 10 170 4509 0000 1920000 25x15emppn 170 4510 0000 1920000 25x20cmpp n 0 170 4511 0000 1920000 25x25emppn D 170 45015 1 960 00000 610 000000 0 000067 000000000 20 0 000 96 192UVTP j 25x 0 0960 I 0000 000 30 051000 150115 2011 260 00 L5mmx 10 0000 9 19210 00000 x 2000 00000000000 19 170 45075 1 00001920000 4x 10100 000000000 192110 000000 0 000067 000000000 20 0000 96 1920 25 10 0 OO 00 01920 0 00000 000 30 510 00 15 2 260 00 L5mmx 10 0000 96 19210 000 00 21000 00000000000 0000000 960 00 000000 170 4514 00000000000 T70 4518 96 19210000 utu 170 4519 96 19271 utu 170 4520 96 1920 00000000 0020 170 4521 0000 96 192 25x 10cm 170 4522 0000 99192UvTP DI 25x 15cm 170 4537 0000000 192000 170 4517 1920000000 170 4518
19. 000 0000000000 0 00000000 00000000000 5 500 60 0 0 000000000 00 000000 000000000 60 C 00 0000000000 000 000000 000000000 0000000 50 700 000000000000 c n 1 000000 0000000000 00000 0000 000000000000 000 2 Movable walli 0 000000 0000 3 000 000000 00000 000000000000 Fixed 0 UVTP 00000 000000000000 D U D wall UVTP O00 000 00 0000 210 00000 000 0000 000 00 0000 00000000000000000000000000000000000 00000000000000000000 Now sf Figure 2 1 Sealing the UVTP tray for gel casting 8 6001 9009000000 00 0000 000000000 60 0000000000 0000000 00 0 000000 00000000 9 3 10 00000000000000000000000000000000000 11 00000000000 000000000 wali 0 0 0000000 00000000000000000 00000 00 0000 12 000000000 DNA 000000000 AM0 dg 00000000 13 00000 4 6mp 00000000000000000 D 0000 0000000000000000 1 00000 00000 00000 0000 2 3 00 0 00000 000 00 000000 4 6q OO 000000000 00 0000 000000000 x 00000000000 301
20. 0000 96 1921 0000 170 4519 96 1921 0000 utu 170 4520 96 1920 00000000 0020 170 4521 0000 99 192UVTPHH D 25x 10cm 170 4522 0000 99 192UVTPHH D 25x 15cm 170 4523 0000 192UVTP I D 25x 20cm 170 4524 0000 1920 25x 25cm 170 4537 0000000 966 1920 000 170 4525 0000 91920000000 170 4526 0000 99192111 26wel 0 75mm 170 4527 0000 96 19200 26wel 15 170 4528 0000 96 19200 5lwel 0 75mm 170 4529 0000 96 19200 5lwel 15101 170 4530 0000 96 1920 000000 2wel 4 075 20 170 4531 0000 96 1910 00000 2well 4well L5rrm D D 00 000 0000 Hann d 164 5050K 96 Basic 0 0 0 025x 164 50501 96 PP Basic 0 0 0 025x 15 1 164 5050M 192 PP 0 00 25x 10 00 164 50503 192 PP Basic 0 025x 15611 164 5050P 192 Basic 0 025x 20cni 164 5050Q 192 PP Basic 025x 25en1 21 BIO RAD 000000000000 00 116 0014 000000000 57 18 03 5811 6270 FAX 03 5811 6272
21. 000000 0 305 0031 000000 1 15 1 TEL 029 852 0835 FAX 029 852 0829 000000 0 222 0033 00000000 2 7 3 TEL 045 476 0351 045 467 0350 000000 0 465 0093 000000000 3 121 1 052 702 2358 FAX 052 702 2812 00000 0532 0025 000000000 1 14 11 06 6308 6568 FAX 06 6308 3064 00000 0 812 0013 0000000000 2 18 30 092 475 4856 092 475 4858 x 0000000000 TEL 03 5811 6271 FAX 03 5811 6272 http discover bio rad co jp M5237 0404
22. 000000000 0000 00000000000000000 000000 0 0 Moovi 0000 00 0000 0 300 500 0 00 0 000 0000000000 0000 000 10 0 00000000000000 000 00000 0000 DD d 0000000 00000000000 0000000000000 000000 0000 00000 1 000000000 00 0000000 000000 00000 000000000 D 0000 00000000 000 00 00 000 000 000 0000000000 D 0000 000 0000000 26 00000000 1 00000000000000 0 0 00 54 o minm 0 0 150 3000000000 00000000000 t 2 0000000000000 9490 000 00100 300 0000000000 0 0 00000000000000000000000000000 000000 00000000 000000000 00000000000000 3 2000000000000 0 9 900 000000000 4 000000000000009 000000000000000000000 5 DNAD O 00000000 000000 025403020 360011 00000000 O 00000000000 80211 250 74000 00 64 0 UVIPIDVIJ 0 0000000000000 000000000000000000000
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