『 レビス ® C‐ペプチド－マウス（U タイプ） 』取扱説明書
Contents
1. C U AKRCP 031 Mouse C peptide U type ELISA KIT AKRCP 031 8hibayagi Co Ltd Gunma Japan 377 0007 1062 1 TEL 0279 25 0279 FAXX 0279 23 0813 lt E mail gt syc 1nfo shibayagn co D lt URL gt http www shibayag1 co D2. 2 8 C C CTMB 2 8 C D CC E 2 8 H 1M HzSO 2 8 C
3. 1 C C 1 2 C 4 C 2 CC C 2 30 TMB 450nm
4. 35 C pH 6 5 7 5 80mg dL
5. C replace Na K ATPase Des 27 31 C C 37 8 5 C C C
6. 20 25 C 2 a 10 og m1l 3 000 1 500 750 93 8 46 9 0 1 150 150 mr er 0 150 w1 150 150 150 150 150 150 150 150 96 3 40g1 10Z1 50g1 0 20 25 C 2 gt 100 3 C 50g1
7. A 96 96 wells 8x12 1 B C 6 000 pg m1 500 1 1 C 60m1l 1 D CC 100 1 1 EE 100 1 1 F TMB 12ml 1 H 1M HzSO4 12m1 1 1 10 x 100m1 1 4 1 7 10 50 1 100 3800 1 Eppendorf multipette plus 50 1
8. 2 40 1 10 1 3 50w1 4 5 20 25 2 6 3 lt 7 CC 50 1 4 8 20 25 2 9 3 lt
9. 80mg dL 80mg dL 2 5 35 11 1 3 lt
10. 6 3 1 EN S S k C C 1 29 2 31 C C A B S S
11. 10 50 1 11 20 25 C 30 12 3 lt 13 50 1 CK 14 20 25 C 30 15 50 1 16 450nm 620nm 600 650nm 7
12. 10 x 10 x 2 8 C 3 9 1 1 96 0 4m sec 830 Web 10 C
13. 3 Q 1 A 1 Q 2 A 2 Q amp A 15 Web Web Q amp A
14. 2 Vortex 600 1 200 rpm 96 Web 96 450 10 nm 620nm 600 650nm 8 20 25 C 2 6 B CC 6 000 pg ml B C 6 000 pg m1l C pg ml 150 1 1501 3 000 3 000 pg ml
15. Strip 1 amp 2 Strip 3 amp 4 Strip 5 amp 6 Strip 7 amp 8 Strip 9 amp 10 Strip 11 amp 12 A 83 000 pg ml 1 9 17 25 33 B 1 500 pg ml 2 10 18 26 34 C 750pg ml 3 11 19 27 35 D 375 pg ml 4 12 20 28 36 E 188pg ml 5 13 21 29 37 F 93 8pg ml 6 14 22 30 38 G 46 9 pg ml 7 15 23 31 39 H 0 8 16 24 32 40 12 1 X pg m1l Y Web ELISA pg m1 5 C
16. 2 3 C 94 0 104 2 3 R2 1 0 14 Q amp A 1 2 3 4 5 6 7 46 9 pg ml OD OD 3 4 6 CV 1 2
17. 2 0 1 1 ELISA 20 lt 25 0 4m sec 30 9 0 4m sec Web 6
18. 3 300u1 ee 9 pg7mD OD OD AE 1 5 25ml 7 600 1 200rpm 10 3 1 2 3 4 5 6 7 8 9 10 11 12 H
19. C 10 9M G NaT KK ATPase NO IGEF II NPY C I C hyperfiltration I C C C 27 31 Des 27 31 C
20. 620nm C 5 ELISA 1
21. 3 4 Blank Ne 100 1000 10000 SUNRISE RAINBOW TECAN Cpeptdelpgmll 18 46 9 3 000 pg ml 5 234 5 15 000pg m1 ELISA C C 1 2 100 C 89 C 85 3 000 pg ml 8 2 C V 5 4 3 4 CV 5
22. 20 25 C 2 gt 100 ee 1 1 3 501 20 25 30 3 TMB TMB 501 2025 30 1M HzSO4 50 1 450nm 620nm 600 650nm 10
23. 150 1 150g1 1 500 1 500 pg ml 150 1 1501 750 750 pg ml 150 1 1501 375 875 pg ml 1501 150 1 188 188 pg ml 1501 1501 93 8 93 8 pg ml 150 1 150p1 46 9 0 1501 0 D CC 100 1 C 100 E 100 1 C 100 10 x 10 x 10 100ml 10 x 900ml 96 A 96 2 8C B CC 6 000 pg m1
24. 2014 10 31 C U Web Q amp A 13 x 10cm 1 C 5 C 10 1 1 96 2 2 8C
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