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2. Ra 731 8180 ES 1 731 8171 EV 1 731 8190 1325 731 8250 1327 750 0037 BioLogic 750 0037 BioLogic HR
3. 18 25 90 100V 50 60Hz
4. www bio rad com Introduction xxi Ordering and General Information
5. lt gt MSDS Material Safety Data Sheet MSDS MSDS 3 http www bio rad com PRTR
6. FETE C 9 iG 208 ye 2 PCR AD TINEA I Ew 2 RE DN ae ee ee a C 2 US hie www bio rad
7. www bio rad com genomics www bio rad com Workflows xi Gene Modulation 191725 AW AEM Description Applications DNA RNA Technologies Related Products siRNA shRNA ass Poo 2717 1 I I 7 III I 7 7 TransFectin lipid reagent p 389 siLentFect lipid reagent for RNAi p 388 Gene Pulser MXcell p 392 Gene Pulser p 390 Gene Pulser Xcell p 394 p 396 Gene Pulser MicroPulser p 397 Helios Gene Gun D 399 PDS 1000 He p 401 ReadyPrep 2 D p 11 ReadyPrep Total
8. 5 600 SDS PAGE Western Blotting RNAi PCR PCR Bio Rad Research Solution Seminar Bio Rad Research Solution Seminar
9. xX Introduction www bio rad com Ordering and General Information http warranty registration bio rad co jp registration htm 1
10. eRadiations FAQ FAQ TechCall Easy to Use Solutions Easy to Use Solutions
11. 7 www bio rad com Workflows xv Protein Purification Description Applications Technologies Related Products Ba sae Experion D 152 Tetra p 211 TGX p 219 Criterion p 221 Flamingo p 240 GelDoc XR Plus p 309 ChemiDoc XRS Plus p 309 ChemiDoc MP p 307 SmartSpec Plus D 26 RC DC p 38 ProteoMiner Enrichment 0 17 ReadyPrep
12. Experion p 152 Criterion p 221 Criterion p 246 Universal p 208 ChemiDoc MP p 307 Oriole It 7 LR Hl p 240 SmartSpec Plus p 26 Sees 1 2717S bo gt 7 gt FRE YO BRA a rr r AN LL F y ReadyPrep 2 D p 11 ReadyPrep p 13 491 p 275 CFX96 Touch PCR p 413 ProteOn XPR36 p 328 Profinia p 111 Profinity eXact fusion tag system 0 69 Profinity IMAC GST p 64 BioLogic DuoFlow p 124 xiv Workflows
13. a 8 E ESI _ Pare ey Vee NGS ng kg ao www bio rad com chromsystems gt Ni e Profinia p 111 Experion p 152 Profinity eXact fusion tag system p 69 Experion Pro260 p 156 Profinity IMAC GST p 64 Tetra p 211 UNOsphere 0 p 46 Criterion p 246 UNOsphere SUPrA A 0 74 Universal p 208 100 0 53 SmartSpec Plus 0 26 HT HTP
14. http www bio rad com 2012 4
15. 2 2 py POD AI iar o o xe enmeor7 oOy 1 1 1 0 ae D Oi 9 Z Vee lt i 1 D 2 D SELDI 5 AGS ge Ble Nes www bio rad com Workflows xix Ordering and General Information Ordering and General Information
16. www bio rad com sqmm 8 AR 8 8 S See Also CR EE Poise SE E raiO a 7 5X10cm 1 165 8040 4 170 3931 ee 1 2 5 170 3812 u T tak II O 170 3919
17. siRNA shRNA TC10 p 24 siRNA shRNA Gene Pulser MXcell p 392 Gene Pulser p 390 Gene Pulser Xcell 0 394 TransFectin lipid reagent p 389 siLentFect lipid reagent for RNAi p 388 Helios Gene Gun p 399 PDS 1000 He Hepta p 401 iv Workflows www bio rad com
18. TechCall 03 6404 0331 life_ps_jp bio rad com xxvi Introduction www bio rad com Ordering and General Information http www bio rad com PDF
19. PCR ELISA Plus p 248 Bio Plex200 p 342 iMark p 377 C1000 Touch p 407 PCR p 420 PCR p 446 785 p 257 TG De a e P ProteOn XPR36 D 328 CFX384 Touch PCR p 412 PCR p 420 PCR p 446 Criterion p 246 HC p 206 785 0 257 eC A4 7 YF EORR 02N NO 12 120
20. 422 www bio rad com XXXIII Hon AFR PCR PCR iScript Advanced cDNA Synthesis kit iScript RNA 423 PCR PCR SsoAdvanced SYBR Green Supermix Bio Rad Sso7d PCR SYBR 433 PCR EpiQ Chromatin Analysis 444 XXXIV www bio rad com Abbreviations 474 Trademarks and Legal Notices 475 References 477 47
21. Profinia amp Advanced PCR ProteOnXPR36 Antibody practice ProteOn XPR36 BioLogic DuoFlow Mi Technical Transfer BioLogic DuoFlow ProteOn XPR36 Bio Plex Bio Plex 1 Bio Plex Bio Plex 3D Basic DuoFlow
22. Tel 03 6404 0331 Easy to Use Solutions Easy to Use Solutions 1 1 Basic Oo 1 PCR FastPCR PCR
23. AA KIR UV 1
24. Bio Plex Bio Plex 1 2 3 PCR PCR Bio Plex Bio Plex ProteOn XPR36 SELDI www bio rad com Introduction xxv Ordering and General Information Bio Rad Boot Camp Bio Rad Boot Camp 0 amp A
25. Tetra 8 000 9 O 170 3934 3 8 000 165 8040 2 798 47715 27 244 FAN Y 77 18 22 000 z 170 3812 48 000 n Tetra 3 170 3934 3 i i 5 P258 259 Pd www bio rad com 253
26. 2 1 3 170 3933 PAE o 98 CH BSRCRET SV A IVAIMBONAAT ARIA fetter terete eee erence e eee E 1 P264 J E B UO 4 1 Tetra 1 i E ao Rimak p er ne eee __ _ eee eet Voce hee Ta Neen aoe 7 ___ __ _ gt _____ tie 3 Ordering Information as _ Aa amp 1 gt 170 3930JA 92 000 i VM 77H FT FTA PY V 17777VC g
27. Turbo p 249 SD p 251 Bio Plex200 p 342 GT p 299 ProteOn XPR36 p 328 CFX96 Touch PCR p 413 PCR p 420 PCR p 446 785 p 257 DNA RNA ema Cle Sey 7 ie So ae ayn 7 3 int I i EAE dsl Cla RRA RIRN CRESS EIES OES Ge www bio rad com genetransfer avai www bio rad com genomics www bio rad com Workflows xiii Protein Interaction Analysis Description Applications Technologies Related Products
28. http www bio rad com www bio rad com Workflows vii DNA Analysis DNA Description Applications 2 Technologies PCR Related Products GT p 280 p 286 ChemiDoc XRS Plus p 309 QuantityOne1 D 0 325 p 243 C1000 Touch p 407 PCR p 420 PCR p 446 ImageLab p 323 ChemiDoc MP p 307 DNA DNA QuantumPrep miniPrep p 4 100 p 53 SmartSpec PlIus 0 26 Experion
29. 1 D 2 D 1 7 ks BioLogic DuoFlow p 124 Profinia p 111 Nuvia 51844 p 46 CHT 0 57 P p82 i12 IEF p 160 Tetra p 211 Criterion p 221 II XL p 230 Basic p 205 xviii Workflows www bio rad com EXOQuest p 319 Bio Plex200 p 342 p 247 ChemiDoc MP p 307 Pharos FX p 316 ProteOn XPR36 p 328 Gel Doc EZ p 312 ImageLab p 323 al
30. 179 185 DNA 31 8 186 DNA 42 191 43 197 45 201 50 203 56 209 62 64 69 On Column 74 77 Ready to Use 79 81 84 86 88 211 221 226
31. 170 6601 3550 170 6638 3550 UV 170 6621 450 170 6750 550 168 1002FM Foodmark 2013 7 168 1002 680 2013 7 168 1101 680XR 2013 7 170 6850 Benchmark 170 6930 Benchmark Plus 2013 8 170 7172 Lumimark 170 9500 Ultramark 170 9501 Twister 170 6966 6967 6968 6969 Fluoromark 170 6680 1250 Immunowash 170 6540 1550 EIA 170 7009 1575 Immunowash S N E EA EC 170 7009 1575 Immunowash S N
32. 307 xxx www bio rad com Hon AFR ProteOn XPR36 ProteOn HTE His Tag HTG His Tag 333 Bio Plex Bio Plex Data Pro Bio Plex 346 Bio Plex Bio Plex Pro Cancer Biomarker I 16 Plex 16 359 Bio Plex Pro TGF TGF 1 2 3 369 Bio Plex Pro GIllTh17 8 Plex B IL 21 IL 22 IL 31 IL 33
33. 236 237 243 RBA 244 245 249 254 ii Contents in Detail www bio rad com 257 258 263 264 267 269 270 271 272 275 27 280 287 294 299 301 1 360 TGF 8B 370 373 374 1 376 380 381
34. Tel 03 6404 0331 Fax 03 6404 0334 Mail life_ps_jp bio rad com Tel 03 6361 7010 Fax 03 5463 8480 www bio rad com Introduction xxvii xxviii Introduction www bio rad com I990018 299001 3 M i12 IEF 12 160 PVDF 260 4 MI gt ChemiDoc MP ChemiDocMPY AF Ald Western Blotting
35. CHEF DRIII 170 3716 FIGE MAPPER SYSTEM 165 5034 GS GeneLinker UV 165 3600 3601 3602 3604 165 1005 SS 165 1745 543 148 0200 0231 BioFocus 3000 148 1200 1211 1202 BioFocus LIF Detector 171 000001 Bio Plex100 5 SELDI PBS II CBIS8 7JOOTC PBS II c PCI 1000 PCS 4000 2013 12 Z500 0013J ProteinChip SELDI system Personal Edition 2013 12 Z500 0011J ProteinChip SELDI system Enterprise Biomarker Edition 2013 12 Z500 0011BPS ProteinChip SELDI system Enterprise Biomarker BPS Edition 2013 12 xxii Introduction www bio rad com Ordering and General Information DNA 170 6700 Gene Cycler 170 8720 8722 8724 8726 iCycler 2013 12 PTC 0160 0196 PTC 100 824 7 PTC 1160G PTC 1196G PTC 100
36. 415 XXXII www bio rad com Hon AFR PCR CFX96 Touch PCR CFX96 Touch PCR CIO000 Touch PCR 6 PCR 413 PCR CFX384 Touch PCR CFX384 Touch PCR 384 PCR 4 412 PCR PCR iScript RT Supermix for RT qPCR Buffer
37. 1 D PCR PCR qPCR Criterion p 221 Experion p 152 ProteoMiner Enrichment p 17 Profinia p 111 Bio Plex p 340 1000 p 405 CFX96 Touch 384 Touch PCR p 412 413 PCR p 420 PCR p 446 785 p 257 WAN PCR QuantityOne1 D p 325 Bio Plex Manager p 344 CFX p 417 ImageLab p 323 ED CHEV COND EERO
38. PTC 0150 0151 0152 MiniCycler PTC 150 PTC 200 DNA Engine PTC 200 M PTC 200G DNA Engine PTC 200 Bio Rad 2014 5 PTC 220 221 DNA Engine Dyad Disiple MJ PTC 221G DNA Engine Dyad Disiple Bio Rad 2015 12 PTC 220G DNA Engine Dyad Bio Rad 2017 12 PTC 0225 DNA Engine Tetrad PTC 225 PTC 0240 DNA Engine Tetrad2 PTC 240 M PTC 0240G DNA Engine Tetrad2 PTC 240 Bio Rad 2017 12 ALD 0211 G Twin Towers Alpha unit ALP 2296 G Moto Alpha 96well ALP 2238 G Moto Alpha 384well 170 8740 iCyclertO PCR 170 9741 MyiO PCR 170 9770 MyiQ Single Color PCR 2014 10 170 9750 9751 978079788 9789 IO5 PCR 2014 10 CFD 3200 Opticon PCR CFD 3220 Opticon2 PCR CFD 3240 Chromo4 PCR MJ CFD 3240G Chromo4 PCR Bio Rad 2013 11 165 2040 620
39. 111 116 124 136 xxix 8 139 2 2 RNA 145 4 DNA 5 DNA 1 8 152 Experion 9 eee 12 17 20 21 160 IEF 168 171 172 2 8 24 174 26 175 30 176
40. 170 7501 670 170 7615 700 170 7930 710 170 7928 SCSI Version GS 800 Calibrated Densitometer 2013 1 170 7980 USB Version 170 7304 GS 250 170 7470 GS 363 170 8300 GS 505 170 7480 GS 525 170 7703 Fluor S Multilmager 170 7728 Fluor S MAX Multilmager 170 7740 Fluor S MAX2 Multilmager 170 7270 InstaDoc II 170 7800 7801 FX 170 7860 FX Pro 170 7855 FX Pro Plus 170 8010 VersaDoc 1000 170 8030 VersaDoc 3000 170 8040 VersaDoc 4000 170 8050 VersaDoc 5000 170 8640 VersaDoc 4000 MP 2017 1 170 8650 VersaDoc 5000 MP 2017 1 170 7520 GelDoc1000 170 8100 8101 8102 8103 GelDo
41. 35 Bio Plex Pro L 6 L 10 TNF a 25 353 xxxi www bio rad com Hon AFR C1000 Touch C1000 Touch 407 T100 96well T100 5 7 409 PCR CFX Connect PCR CFX Connect PCR 2 CFX96 Touch
42. 289 Bulletin sm
43. Overview Finding Your Product ii ii iv vi viii DNA x xii XV xvi XVII 478 514 515 552 S55 cs PCR SELDI Note Due to the nature of new products some items in this catalog may not be available for immediate purchase For more information contact your local Bio Rad sales representative www bio rad com Contents in Detail i E introduction 89 i 89 xx Ordering and General Information 101 110
44. p 61 Gel Doc EZ p 312 CHT 0 57 A p 97 p 104 BioLogic LP p 116 BioLogic DuoFlow p 124 www bio rad com Workflows xvii Protein Separation and Analysis Description Applications 22 wa 7774 gt 5 1 0 C C my 1 D 2 08C786 187 ER Technologies Related Products ProteoMiner Enrichment 0 17 ReadyPrep 0 10 ReadyPrep p 13 491 p 275 p 273
45. etra p 211 LL OR CATAN 2 Bio Plex200 p 342 PharosFX p 316 EXQuest p 319 CFX384 Touch PCR ATL p 413 Oriole It 7 LR Hl p 240 PCR p 420 PCR p 446 vi Workflows www bio rad com DNA Bio Plex Manager p 344 ImageLab p 323 PDQuest 2 D p 326
46. 0 26 x Workflows www bio rad com RT PCR C1000 Touch p 407 S1000 p 408 T100 p 409 iTaq DNA p 430 PCR p 420 PCR p 446 iQ supermix p 440 _ mRNA CL III a CFX Connect PCR p 415 CFX96 Touch PCR p 413 Bio Plex200 p 342 iQ SYBR Green supermix p 437 GelDoc XR Plus p 309 QuantityOne1 D 0 325 iScript one step RT PCR p 426 SsoFast EvaGreen supermix D 434 SsoAdvanced SYBR Green supermix D 433 RNA Gr sar easy to use Fast
47. 0 10 ReadyPrep 0 13 p 273 TRUTA A Flamingo p 240 SmartSpec Plus D 26 RC DC p 38 ChemiDoc XRS Plus p 309 ChemiDoc MP p 307 ImageLab p 323 IMark p 377 Oriole p 240 xvi Workflows www bio rad com FE _ I I I a Zoe le Balu Boke 9 l MERA SIN 0 xe Hoh He
48. 731 8123 2128 HPLC 125 1515 1516 2700 125 1527 1528 1529 1531 5000 HPLC 125 0590 0592 0594 0595 GlycoChrom 125 1680 PC 125 1684 ValueChrom 125 1350 1352 1355 1350 167 0940 0941 Bio DimensionUV VIS 167 1000 1005 1740 UV VIS 167 0900 1790 UV VIS 167 0970 1801 UV VIS 167 0950 1806 UV VIS 167 0458 1755 RI 167 1100 1102 AS 100 T 125 0425 HPLC 165 4761 200 2 0 165 4710 1000 500 165 0554 3000xi 165 5052 200 165 5050 300 165 5054 1000 165 5056 3000 170 3601 760 170 3688 1000 310 0008A 310 0024A
49. EC 2015 8 179 7009 Immunowash 1575 Fast Prep 2005 TechCall Tel 03 6404 0331 Fax 03 6404 0334 E mail life_ps_jp bio rad com 7143 0006 2 1 1 14 B 3 Tel 03 6404 0381 Fax 03 6404 0332 xxiv Introduction www bio rad com Ordering and General Information
50. 382 ELISA 306 319 386 387 390 390 391 398 PCR 322 323 11 328 ProteOn XPR36 328 330 332 Regulatory 333 335 1 340 Bio Plex 342 Bio Plex 342 Bio Plex 349 355 Diabetes 359 Cancer 360 363 404 404 405 1000 409 411
51. Titertube Trans Blot Trans Blot Turbo TransFectin trUView Ultramark UltraRocker UNO UNOsphere UNOsphere SUPrA Verification Probe VersaDoc WesternC WinMelt XcitaBlue Xcluda xMark x Plex XPR Zeta Probe www bio rad com 475 Trademarks and Legal Notices The following trademarks are the property of the companies listed 8 Pette Corning Incorporated 12 Pette Corning Incorporated Adobe Acrobat Adobe Systems Incorporated AKTA GE Healthcare Group Companies Alexa Fluor Invitrogen Corporation American Express American Express Company Beacon Designer PREMIER Biosoft International Benchmate Oxford Worldwide LLC BLAST National Library of Medicine CDP Star Applera Corporation Cell Signaling Technology Cell Signaling Technology logo Cell Signaling Technology Inc Cibacron Ciba Geigy Corporation Coomassie BASF Aktiengesellschaft Costar Corning Incorporated Cy GE Healthcare Group Companies Dell Dell Inc Delrin E l du Pont de Nemours and Co DyNA Chill Labnet International Eppendorf Eppendorf Netheler Hinz GmbH EvaGreen Biotium Inc Excalibur Brinkmann Excel Microsoft Corporation Finnpipette Thermo Fisher Scientific Inc FireWire Apple Inc FPLC GE Healthcare Group Companies Fuji Fuji Photo Film Co Ltd GelGreen Biotium Inc HASP Aladdin Knowledge Systems Ltd Legal Notices H
52. Fda 1 xxv Bio Rad Laboratories Inc Hercules CA m TechCall FAQ Web
53. Phone 82 2 3471 6500 Fax 82 2 3472 1211 Latvia Diamet SIA Riga Phone 871 754 33 09 Fax 871 754 33 31 Email bio rad mt lv Lithuania Ardeola Co Ltd Vilnius Phone 370 52 69 11 29 Fax 870 52 69 11 28 Email ardeola ardeola t Malaysia Chemoscience Malaysia Sdn Bhd Selangor Phone 603 7872 6000 Fax 603 7876 3188 Mexico Bio Rad S A Mexico D F Phone 52 555 488 7670 Fax 52 65 1107 7246 Email Isg_mexico bio rad com The Netherlands Bio Rad Laboratories B V Veenendaal Phone 0318 540666 Fax 0318 542216 New Zealand Bio Rad Laboratories Pty Ltd Auckland Phone 0508 805 500 Fax 64 9 415 2284 Email sales nz bio rad com The Pacific Islands and Papua New Guinea are handled through the New Zealand office Email Export_Pacific bio rad com Norway Bio Rad Laboratories Oslo Phone 23 38 41 30 Fax 23 38 41 39 Email nordic_helodesk bio rad com Palestine Medipharm Co Ltd Beit Sahour Phone 972 2 277 2833 4 Fax 972 2 277 5245 Panama DiaMed Caribbean Inc Miami Phone 1 305 558 4510 Fax 1 305 558 4315 Email diamed bellsouth net Peru J amp B Lab S A C Lima Phone Fax 511 6286958 Email soporte jblabsac com gcomercial jblabsac com Philippines Lifeline Diagnostic Supplies Inc Quezon City Phone 632 376 2617 Fax 632 372 1675 Poland Bio Rad Polska Sp 7 0 0 Warszawa Phone 48 22 331 99 99 Fax 48 22
54. p 152 viii Workflows www bio rad com PCR AES KB PCR qPCR Gel drying _ k T100 p 409 1000 p 405 PCR p 420 PCR p 446 HV p 207 583 0 30 2 304 PCR SNP ELISA r E ma 5 CFX96 Touch PCR p 413 DNA p 186 PCR p 420 PCR p 446 CHEF MapperXA p 288 DCode DGGE p 294 p 286 GelDoc XR Plus p 309 Bi
55. 86 20 87322332 Email sales china bio rad com Wuhan location Phone 86 27 83806255 Fax 86 27 83806265 Email sales china bio rad com Colombia AM Asesoria amp Mantenimiento Ltda Bogota Phone 57 1 6338 61 21 Fax 57 1 627 69 94 Email ventas amltda com Costa Rica Diamed Caribbean Inc Miami Phone 1 305 558 4510 Fax 1 305 558 4315 Email diamedevelia bellsouth net Czech Republic Bio Rad spol s r o Praha Phone 420 241 430 532 Fax 420 241 431 642 Denmark Bio Rad Laboratories K benhavn Phone 44 52 10 00 Fax 44 52 10 01 Email nordic_helodesk bio rad com Dominican Republic DiaMed Caribbean Inc Miami Phone 1 305 558 4510 Fax 1 305 558 4315 Email diamed bellsouth net Ecuador AM TEC LAB Quito Phone 57 1 633 6121 Fax 57 1 633 6121 Email amtec lab amltda com Estonia Quantum Eesti AS Tartu Phone 372 7 301 321 Fax 872 7 304 310 Email quantum quantum ee Finland Bio Rad Laboratories Helsinki Phone 358 0 9 804 2200 Fax 358 0 9 7597 5010 Email nordic_helpdesk bio rad com France Bio Rad Laboratories Marnes la Coquette Phone 01 47 95 69 65 Fax 01 47 95 61 21 The following countries are handled through the French office French Guyana Guadeloupe Martinique Reunion Island The following countries are handled through the Emerging Markets Division Afghanistan Albania Algeria Armenia Azerbaijan Benin Bosnia and
56. HydroTech i12 iCycler iCycler iQ Image Lab iMark Immun Blot Immunowash Immun Star InPlace InstaGene iProof iQ iScript iTaq Kaleidoscope LP Data View Macro Prep MainFrame MAPS Micro Bio Spin Microplate Manager MicroPulser MicroRotofor Microseal MiniOpticon Mini PROTEAN Mini Sub Mini Trans Blot MJ Mini Multiplate MyCycler MyiQ Nuvia One shot Kinetics Opti 4CN Opticon Opticon Monitor Oriole PCR Kleen PDQuest PDS 1000 He Personal Molecular Imager PharosFX PMI Poly Prep Power Bonnet PowerPac Precision Melt Analysis Precision Plus Protein Precision Pro Profinia Profinity Profinity eXact PROTEAN ProteinChip Prot Elec ProteoMiner ProteOn The following are either registered trademarks or trademarks of Bio Rad Laboratories Inc in the U S and or other countries For the most up to date and comprehensive list of Bio Rad trademarks visit www bio rad com ProteOn Manager PureZOL Quantity One Quantum Prep Quick Start RC MC ReadyAgarose Ready Gel ReadyPrep ReadyStrip ReadySub Cell Rotofor 51000 Seque Pro Sequi Blot Sequi Gen ShockPod siLentFect Silver Stain Plus SmartSpec SsoAdvanced SsoFast Sub Cell T100 TC10 Tetrad TGX TGX Stain Free
57. PCR 417 Amplification Software 420 PCR 421 426 PCR 431 PCR 439 PCR 444 PCR 446 PCR 448 459 1 464 Biotechnology Explorer 11 SELDI 472 ProteinChip Array www bio rad com Contents in Detail iii Stem Cell Research EP NAC TA Description Applications Technologies Related Products iPS
58. go to www bio rad com www bio rad com Visit us on the Web For technical support and literature For information on new products events and campaign For the latest news from Bio Rad Os J Us BIO RAD 140 0002 2 2 24 Tel 03 6361 7000 Fax 03 5463 8480 ABR 532 0025 1 14 11 Tel 06 6308 6568 Fax 06 6308 3064 812 0013 2 5 28 Tel 092 475 4856 Fax 092 475 4858 Tel 03 6404 0331 Fax 03 6404 0334 Tel 03 6361 7010 Fax 03 5463 8480 C10421 1203A
59. 059 948 5 578 180 5 922 185 6 162 338 and 6 783 651 and corresponding foreign patents Strep tag technology for western blot detection is covered by U S Patent Number 5 506 121 and by UK Patent Number 2 272 698 StrepTactin is covered by German patent application P 19641876 3 Bio Rad Laboratories Inc is licensed by Institut fur Bioanalytik GmbH to sell these products for research use only Precision Plus Protein standards are sold under license from Life Technologies Corporation Carlsbad CA for use only by the buyer of the product The buyer is not authorized to sell or resell this product or its components Bio Rad Laboratories Inc is licensed by Invitrogen Corporation to sell SYPRO products for research use only under U S Patent Number 5 616 502 Biolistic technology is covered by patents owned by Pfizer Inc The technology is exclusively licensed to Bio Rad Laboratories Inc 476 www bio rad com References Legal Notices cont Notice regarding Bio Rad thermal cyclers and real time systems Purchase of a Bio Rad thermal cycler or real time system conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and for use in human in vitro diagnostics and all other applied fields under U S Patent Number 5 475 610 Claims 1 44 158 160 163 and 167 only or corresponding claims in its non U S counterpart owned by Applera Corpo
60. BJ 1959 Disc Electrophoresis Distillation Products Industries Division of Eastman Kodak Co Porter RR 1953 Partition chromatography of insulin and other proteins Biochem J 53 320 328 Rohringer R and Holden DW 1985 Protein blotting detection of proteins with colloidal gold and of glycoproteins and lectins with biotin conjugated and enzyme probes Anal Biochem 144 118 127 Titani K et al 1977 Complete amino acid sequence of rabbit muscle glycogen phosphorylase Proc Natl Acad Sci USA 74 4762 4766 Woods EF et al 1963 Studies on the structure of myosin in solution J Biol Chem 238 2374 2385 Wu YV and Scheraga HA 1962 Studies of soybean trypsin inhibitor I Physicochemical properties Biochemistry 1 698 705 Amplification PCR Zuker M 2003 Mfold web server for nucleic acid folding and hybridization prediction Nucleic Acids Res 31 3406 3415 www bio rad com 477 Worldwide Distributors and Sales Offices of Bio Rad Laboratories Group Headquarters Bio Rad Laboratories Inc 2000 Alfred Nobel Drive Hercules CA 94547 USA Phone 1 510 741 1000 Toll free 1 800 4 BIORAD Fax 1 510 741 5800 or 1 800 879 2289 Argentina Tecnolab Buenos Aires Phone 0054 1 1 4555 0010 Fax 0054 11 4553 3331 Email bmol tecnolab com ar Australia Bio Rad Laboratories Pty Ltd Sydney Phone 61 2 9914 2800 Fax 61 2 9914 2888 Toll free 1 800 224 354 Email sales aust
61. Co Texas Red Invitrogen Corporation Titertek Titertek Instruments Inc Tri Clamp Alfa Laval Inc Triton Union Carbide Corporation Tween ICI Americas Inc Tygon Norton Company Veriti Applera Corporation Visa Visa International Service Association Volac Bacto Laboratories Pty Ltd Windows Microsoft Corporation Windows Vista Microsoft Corporation xMAP Luminex Corporation xPONENT Luminex Corporation LabChip and the LabChip logo are trademarks of Caliper Life Sciences Inc Bio Rad Laboratories Inc is licensed by Caliper Life Sciences Inc to sell products using the LabChip technology for research use only These products are licensed under U S Patent Numbers 5 863 753 5 658 751 5 436 134 and 5 582 977 and pending patent applications and related foreign patents for internal research and development use only in detecting quantitating and sizing macromolecules in combination with microfluidics where internal research and development use expressly excludes the use of this product for providing medical diagnostic or any other testing analysis or screening services or providing clinical information or clinical analysis in any event in return for compensation by an unrelated party The Bio Plex suspension array system includes fluorescently labeled microspheres and instrumentation licensed to Bio Rad Laboratories Inc by the Luminex Corporation CST antibodies exclusively developed and validated for Bio Ple
62. com www bio rad com Workflows v Biomarker Discovery Description Applications Technologies Related Products DNA RNA RNA DNA ProteoMiner Enrichment 0 17 Aurum RNA 0 2 ReadyPrep total protein p 10 ReadyPrep 0 13 QuantumPrep p 4 Criterion p 221 Experion D 152 Experion Pro260 p 156 Basic p 205 PlusDodeca p 232 Flamingo p 240 TGX p 219
63. protein p 10 Aurum RNA 0 2 Quantum Prep miniPrep p 4 xii Workflows www bio rad com ela e AA 2 PCR I nw i12 IEF p 160 Tetra p 211 TGX p 219 ZetaProbe p 261 CFX96 Touch PCR p 413 ChemiDocXRS Plus p 309 Experion p 152 Oriole p 240 PCR p 420 _ gt 7 gt 2 7 7 I I 2 PCR
64. www bio rad com Lipid Reagent i12 IEF p 160 Pharos FX p 316 Gene Pulser MXcell p 392 Gene Pulser Xcell p 394 p 397 1 I 0 152 EN COB CI C ES 2 AO oD
65. 331 99 88 Portugal Bio Rad Laboratories Lda Amadora Phone 351 21 4727700 Fax 351 21 4727777 Email Isg_pt bio rad com Romania Bio Rad Laboratories Bucuresti Phone 40 21 210 1703 Fax 40 21 210 1507 Email office bio rad ro Russia Bio Rad Laboratorii 000 Moscow Phone 7 495 721 14 04 Fax 7 495 721 14 12 Email post bio rad ru Singapore Bio Rad Laboratories Singapore Pte Ltd Phone 65 6415 3188 Fax 65 6415 3189 Email sales singapore bio rad com Brunei is handled through the Singapore office Slovak Republic Bio Rad Laboratories Bratislava Phone 421 243 634 487 Fax 421 243 634 488 South Africa Bio Rad Laboratories Ltd Parklands Phone 27 0 861 246 723 Fax 27 11 327 2954 Spain Bio Rad Laboratories S A Madrid location Phone 34 91 5905200 Fax 34 91 5905217 Email customerservice_spain bio rad com Bio Rad Laboratories S A Barcelona location Phone 34 93 479 15 10 Fax 34 93 478 15 00 Email customerservice_spain bio rad com Sri Lanka P amp T Trading P Ltd Colombo Phone 00 9411 2508888 Fax 00 9411 2508668 Sweden Bio Rad Laboratories AB Sundbyberg Phone 08 555 12700 Fax 08 555 12780 Email nordic_helodesk bio rad com Switzerland Bio Rad Laboratories AG Reinach Phone 061 717 95 55 Fax 41 61 717 95 50 Product Support 061 717 95 60 Email swisS bio rad com Taiwan Bio Rad Laboratories Taiwan Ltd Taipe
66. 51 Protein measurement with the Folin phenol reagent J Biol Chem 193 265 275 Electrophoresis and Blotting Black JA and Leaf G 1965 The action of cyanogen bromide on horse heart cytochrome c and horse heart myoglobin Biochem J 96 693 699 Brew K et al 1967 Comparison of the amino acid sequence of bovine a lactaloumin and hens egg white lysozyme J Biol Chem 242 3747 3749 Brown JR 1975 Structure of bovine serum albumin Fed Proc 34 591 Corran PH and Waley SG 1974 The tryptic peptides of rabbit muscle triose phosphate isomerase Biochem J 139 1 10 Davis RP 1961 Carbonic anhydrase pp 545 564 in The Enzymes Vol 5 2nd edn Boyer PD ed Academic Press New York Fowler AV and Zabin 1977 The amino acid sequence of B galactosidase of Escherichia coli Proc Natl Acad Sci USA 74 1507 1510 Gatti RA et al 1984 Multiple uses of Southern blots Biotechniques 2 148 155 Gottlieb M and Chavko M 1987 Silver staining of native and denatured eucaryotic DNA in agarose gels Anal Biochem 165 33 37 Jolles P 1969 Lysozymes a chapter of molecular biology Angew Chem Int Ed Engl 8 227 239 patents specified in the product insert to use only the amount purchased for the purchaser s own internal research when used with the separate purchase of Licensed Probe No other patent rights are conveyed expressly by implication or by estoppel Further information on purchasing licenses may be obtained from t
67. 8 478 515 Abbreviations Abbreviations 1 D 2 D A niger AFIGE AFLP AG ANSI AP ASQ BCA BSA CCD CDGE cDNA CHCA CHEF CIP CV DGGE DHFR dNTP ddNTP DIGE DMSO DNA dsDNA DTT E coli EAM EDTA ELISA ESI MS EtBr FEP FIGE FISH FPLC FRET GAMP GAPDH GFP GLC GLP GMO GMP One dimensional Two dimensional Aspergillus niger Asymmetric field inversion gel electrophoresis Amplified fragment length polymorphism Analytical grade American National Standards Institute Alkaline phosphatase American Society for Quality Bicinchoninic acid Bovine serum albumin Charge coupled device Constant denaturing gel electrophoresis Complementary deoxyribonucleic acid a Cyano 4 hydroxycinnamic acid Clamped homogeneous electrical field Clean in place cleaning in place Coefficient of variation Denaturing gradient gel electrophoresis Dihydrofolate reductase Deoxynucleotide triphosphate Dideoxynucleotide triphosphate Difference gel electrophoresis Dimethyl sulfoxide Deoxyribonucleic acid Double stranded DNA Dithiothreitol Escherichia coli Energy absorbing molecule Ethylenediaminetetraacetic acid Enzyme linked immunosorbent assay Electrospray ionization mass spectrometry Ethidium bromide Fluorinated ethylene propylene Field inversion gel electrophoresis Fluorescence in situ hybridization Fast protein liquid chromatography
68. Fluorescence resonance energy transfer Good automated manufacturing practices Glyceraldehyde 3 phosphate dehydrogenase Green fluorescent protein Gas liquid chromatography Good laboratory practices Genetically modified organism Good manufacturing practices GQT GST H wingei HASP HPLC HPRT HRP HTF ICM ID IDA IEF IEP IgG IMAC IPG LCD LED LNA mAb MANOVA MBP MCS MMLV mRNA MW NASBA NIST NTA OD ODC OQ PACE PAGE PBS PCR Genetic quality tested Glutathione S transferase Hansenula wingei Hardware against software piracy High performance liquid chromatography Hypoxanthine guanine phosphoribosyltransferase Horseradish peroxidase High throughput fluidics Instrument control module Inner diameter Iminodiacetic acid Isoelectric focusing Immunoelectrophoresis Immunoglobulin G Immobilized metal affinity chromatography Immobilized pH gradient Isopropyl 6 D I thiogalactopyranoside Installation qualification Infrared International Organization for Standardization Liquid crystal display Light emitting diode Locked nucleic acid Monoclonal antibody Multivariate analysis of variance Maltose binding protein Multiple cloning site Moloney murine leukemia virus Messenger RNA Molecular weight Nucleic acid sequence based amplification National Institute of Standards and Technology Nitrilotriacetic acid Optical density outside diameter Ornithine decarboxylase Operational qualifi
69. Herzegovina Bulgaria Burkina Fasso Burundi Cameroon Cape Verde Central African Republic Chad Congo Croatia Djibouti Gabon Georgia Ghana Guinea Guinea Bissau Guinea Konakry Ivory Coast Kirghizstan Macedonia Madagascar Mali Mauritania Moldavia Mongolia Montenegro Morocco Niger Nigeria Russia Rwanda Sao Tom Senegal Serbia Sierra Leone Slovenia Syria Tadjikistan Togo Tunisia Turkmenistan Uzbekistan Zaire The following countries have local offices or distributors but are also handled through the Emerging Markets Division Angola Belarus Botswana Ethiopia Kazakhstan Kenya Lesotho Liberia Malawi Mozambique Namibia Romania Slovak Republic Somalia Sudan Swaziland Tanzania Turkey Uganda Ukraine United Arab Emirates Zambia Zimbabwe Direct initial inquiries to Bio Rad Emerging Markets Division Phone 33 147 95 62 85 Fax 33 147 95 61 81 Germany Bio Rad Laboratories GmbH Munchen Phone 49 89 31 8840 Fax 00 800 27 32 96 73 37 Email orders central europe bio rad com Greece Interlab Ltd Konstantinos Oikonomou Kallithea Athens Phone 30 210 9532 220 Fax 30 210 9582 221 Email info interlab gr Hong Kong Bio Rad Pacific Ltd Quarry Bay Phone 852 2789 3300 Fax 852 2789 1290 Email sales hongkong bio rad com Hungary Bio Rad Hungary Ltd Budapest Phone 36 1 459 6100 Fax 36 1 459 6101 Email info_hungary bio rad com India Bi
70. c2000 170 8065 GelDocEQ 170 8170 8171 GelDoc XR 2014 USB 310SNAPS Gel Snap UV 170 8102 ChemiDoc 170 8100 ChemiDoc 2000 170 8064 ChemiDoc EQ 170 8170 8171 ChemiDoc XRS 2014 USB 165 7040 ProteomeWorks Spot Cutter 165 7044 ProteomeWorks Spot Cutter RTS2000 RTS2000MP 165 2257 PDS 1000 He 165 2075 165 2087 165 2098 165 2105 165 2107 2108 Il Plus 165 2101 E Coli DNA BST 1000 0200 0300 0400 BaseStation100 51 DNA 169 0002 0003 VersArray ChipReader System www bio rad com Introduction xxiii Ordering and General Information 170 2501 SmartSpec3000
71. cation Programmable autonomously controlled electrodes Polyacrylamide gel electrophoresis Phosphate buffered saline Polymerase chain reaction PDA PFGE p pNPP PQ PRINS PVDF PVP QC qPCR RFLP RFU RISC RNA RNAi RPM RT PCR rt PCR Personal data assistant Pulsed field gel electrophoresis Isoelectric point p Nitrophenyl phosphate Performance qualification Primed in situ labeling Polyvinylidene fluoride Polyvinylpyrrolidone Quality control Quantitative PCR Restriction fragment length polymorphism Relative fluorescence unit RNA induced silencing complex Ribonucleic acid RNA interference Resistor pulse modulation Reverse transcription polymerase chain reaction Real time polymerase chain reaction S cerevisiae Saccharomyces cerevisiae 5 pombe SBS SDS SEC SELDI siRNA SNP SPA SPR SoG SSCP ssDNA TBP TBS TFA TSE TTGE TTL U S FDA UV VGA Schizosaccharomyces pombe Society for Biomolecular Screening Sodium dodecyl sulfate Size exclusion chromatography Surface enhanced laser desorption ionization Small interfering RNA Single nucleotide polymorphism Sinapinic acid Surface plasmon resonance Saline sodium citrate Single stranded conformation polymorphism Single stranded DNA Tributylphosphine Tris buffered saline Trifluoroacetic acid Transmissible spongiform encephalopathy Temporal temperature gradient electrophoresis Transistor transistor logic United States Foo
72. d and Drug Administration Ultraviolet Video graphics array 474 www bio rad com Trademarks and Legal Notices Affi Gel Affi Prep AG Alpha Aminex AmpliSize AnyGel Any kD Aurum Bio Beads Bio Dot BioFrac Bio Gel BioLogic BioLogic DuoFlow BioLogic DuoFlow Maximizer BioLogic DuoFlow Pathfinder BioLogic DuoFlow QuadTec BioLogic Maximizer BioLogic QuadTec Bio Lyte Bio Plex Bio Plex Manager Bio Plex Pro Bio Rad Bio Rex Bio Safe Bio Scale Bio Sil Bio Silect Bio Spin Biotechnology Explorer C1000 C1000 Manager C1000 Touch Certified CFT CFX96 Touch CFX384 Touch CFX Connect CFX Manager CFX96 CFX384 Checkmark CHEF DR CHEF Mapper Chelex ChemiDoc Chill out Trademarks and Legal Notices CHT CleanCut Concord C P Lift Criterion Criterion Stain Free DC DCode DNA Engine DNA Engine Dyad DNA Engine Tetrad Dodeca Dual Alpha Dyad DynaLoop Easy Cap EasyPack Econo Econo Column Econo Pac EpiQ Experion EXQuest EZ Load EZLogic EZ Micro Flamingo FPQuest Frame Seal Freeze N Squeeze GelAir Gel Doc Gene Pulser Gene Pulser MXcell Gene Pulser Xcell GeneShot GS 800 Hard Shell Helios Hepta Hot Bonnet
73. he Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA SYBR is a trademark of Molecular Probes Inc Bio Rad Laboratories Inc is licensed by Molecular Probes Inc to sell reagents containing SYBR Green for use in real time PCR for research purposes only EvaGreen is a trademark of Biotium Inc Bio Rad Laboratories Inc is licensed by Biotium Inc to sell reagents containing EvaGreen dye for use in real time PCR for research purposes only Hard Shell plates are covered by one or more of the following U S patents or their foreign counterparts owned by Eppendorf AG U S Patent Numbers 7 347 977 6 340 589 and 6 528 302 Primer3 includes software developed by the Whitehead Institute for Biomedical Research Electrophoresis and Blotting cont Kassell B and Laskowski M Sr 1965 The basic trypsin inhibitor of bovine pancreas V The disulfide linkages Biochem Biophys Res Commun 20 463 468 Laemmli UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 Lerman LS and Silverstein K 1987 Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis Methods Enzymol 155 482 501 Li JK et al 1987 Rapid alkaline blot transfer of viral dsRNAs Anal Biochem 163 210 218 Merck Index 1989 Monograph 948 11 149 Merck amp Co Inc New Jersey Ornstein L and Davis
74. i Phone 886 2 2578 7189 Fax 886 2 2578 6890 Email sales taiwan bio rad com Pantech Instruments Co Ltd Taipei Phone 886 2 2731 9211 Fax 886 2 2741 5583 Thailand Bio Rad Laboratories Ltd Lumpini Pathumwan Bangkok Phone 662 651 8311 Toll free 1800 88 22 88 Fax 662 651 8312 Email sales thailand bio rad com Turkey Bio Rad Turkey Istanbul Office Florya Istanbul Phone 90 212 573 19 75 76 77 Fax 90 212 573 15 10 Ukraine Bio Rad s Representative Office Kyiv Phone 38 044 559 96 43 Fax 38 044 559 46 13 Email office bio rad com ua United Arab Emirates Bio Rad SNC Sharjah Phone 971 6574 8328 Fax 971 6574 9273 The following countries are handled through the United Arab Emirates office Bahrain Egypt Jordan Kuwait Lebanon Libya Oman Pakistan Qatar Saudi Arabia Yemen United Kingdom Bio Rad Laboratories Ltd Hemel Hempstead Hertfordshire Phone 44 0 20 8328 2000 Free phone 0800 181134 Fax 44 0 20 8328 2550 Alpha Analytical Larne Co Antrim Northern Ireland Phone 02828 260558 Fax 02828 260548 lreland has a local distributor but please direct initial inquiries to the Hemel Hempstead office Uruguay Biodiagnostico Cabinsur SA Phone Fax 598 2487 1537 Email bio biodiagnostico com uy Venezuela Loginca C A Colinas de Bello Monte Caracas Telefax 58 212 751 9376 5732 9189 Email loginca cantv net For up to date information
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76. o Plex200 p 342 DNA DNA sui Cee DNA DNA www bio rad com Workflows ix Gene Expression Description RNA Applications Technologies RNA Related Products Aurum RNA p 2 RNA ANI I ILIXIL I Experion 0 152 Experion RNA p 156 GT p 280 p 286 ChemiDoc XRS Plus p 309 ChemiDoc MP p 307 QuantityOne1 D p 325 ImageLab p 323 SmartSpec Plus
77. o Rad Laboratories India Pvt Ltd Gurgaon Phone 91 124 4029300 Fax 91 124 2398115 Toll free LSG tech support 1 800 1801224 Email sales india bio rad com support india bio rad com The following countries are handled through the India office Bangladesh Sri Lanka Nepal Maldives Mauritius Seychelles Indonesia PT Sciencewerke Jakarta Selatan Phone 62 21 7203678 Fax 62 21 7398723 Email diastika dnet net id Ireland Alpha Technologies Ltd Blessington Co Wicklow Phone 353 45 865440 Fax 353 45 86544 1 Israel Bio Rad Laboratories Ltd Rishon Le Zion Phone 03 963 6050 Fax 03 951 4129 Email israel_sales bio rad com Italy Bio Rad Laboratories S r l Milan Phone 39 02 216 091 Fax 39 02 21609399 Email Isg_info_it bio rad com Cyprus and Malta are handled through the Milano office Japan Tokyo location main office Bio Rad Laboratories KK Shinagawa ku Phone 81 3 6361 7000 Fax 81 3 5463 8480 Osaka location Yodogawa ku Phone 81 6 6308 6568 Fax 81 6 6308 3064 Fukuoka location Hakata ku Phone 81 92 475 4856 Fax 81 92 475 4858 Kazakhstan Bio Rad Representative Office Kazakhstan Almaty Phone 7 727 2506026 Fax 7 727 3943635 Email bio rad_kz bio rad ru Korea Bio Rad Korea Ltd Seoul Phone 82 2 3473 4460 Fax 82 2 3472 7003 Email sales korea bio rad com support korea bio rad com BMS Bio Medical Science Co Ltd Seoul
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79. ration No right is conveyed expressly by implication or by estoppel under any other patent claim such as claims to apparatus reagents kits or methods such as 5 nuclease methods Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Bio Rad s thermal cyclers are covered by one or more of the following U S patents or their foreign counterparts owned by Eppendorf AG U S Patent Numbers 6 767 512 and 7 074 367 Bio Rad s real time thermal cyclers are licensed real time thermal cyclers under Applera s U S Patent Number 6 814 934 B1 for use in research human in vitro diagnostics and all other fields except veterinary diagnostics Practice of the patented 5 Nuclease Process requires a license from Applied Biosystems The purchase of these products includes an immunity from suit under References Sample Preparation Chomczynski P and Sacchi N 1987 Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction Anal Biochem 162 156 159 Vuillard et al 1995 Enhancing protein solubilization with nondetergent sulfobetaines Anal Biochem 230 290 294 Sample Quantitation Bradford MM 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding Anal Biochem 72 248 254 Lowry OH et al 19
80. t 7 gt 7 1 FIVAIVE 2 yo A 1 L au on H 170 3935JA 76 000 1 _ gt Ny 1 2 1 Pen Ordering Information HC 164 5052W PP HC 200 000 HC 170 3931 1 11 000 170 3932 7 5X10 5cm 50 6 500 TH 17033933 8X11cm 4 5 500 ZZ 170 3812 48 000 170 3919 Tetra
81. x phosphoprotein and total target assays Profinity eXact vectors tags and resins are exclusively licensed under patent rights of Potomac Affinity Proteins This product is intended for research purposes only For commercial applications or manufacturing using these products commercial licenses can be obtained by contacting the Life Science Group Chromatography Marketing Manager Bio Rad Laboratories Inc 6000 Alfred Nobel Drive Hercules CA 94547 Telephone 1 800 4BIORAD 1 800 424 6723 The composition and or use of the T7 expression system is claimed in one or more patents licensed to Bio Rad by Brookhaven Science Associates LLC A separate license is required for any commercial use including use of these materials for research or production purposes by any commercial entity The SELDI process is covered by U S Patent Numbers 5 719 060 6 225 047 6 579 719 and 6 818 411 and other issued patents and pending applications in the U S and other jurisdictions Expression and purification of GST fusion proteins may require a license under U S Patent Number 5 654 176 assignee Chemicon International CHEF U S Patent Number 5 549 796 issued to Stanford University is exclusively licensed to Bio Rad Laboratories Inc Purchase of Criterion XT Bis Tris gels XT MOPS running buffer XT MES running buffer XT MOPS buffer kit and XT MES buffer kit is accompanied by a limited license under U S Patent Numbers 6 143 154 6 096 182 6
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