大麻草の種子形態 - YAKUGAKU ZASSHI
Contents
1. 238 Vol 130 2010 6 expected amount of cannabis products derived from illegal cannabis plant HA non narcotic cannabis z identificati TOW E RELE lnti o gt i ivati 4 outdoor cultivation O MEIEN a 7 i i 8 usage of medicinal cannabis 5 germination and growth characteristics ls the sum Fig 1 Itemization of Queries on Cannabis Arisen from January 2000 to March 2009 THC 1 2000 1 2009 3 312. 2 3 5 triphenyl 2H tetrazolium chloride TTC 20 25 C 12 24 7 10 4 2 3 4 2 Toc THC
3. 242 Vol 130 2010 100 80 60 Rooting 40 Cotyledon expansion 20 0 T 0 2 4 6 8 10 12 Days 100 After 8 years preservation 80 E 60 210 C atmosphere 5 E 10 C vacuum 8 40 t 1 C atmosphere x E 1 C vacuum 20 20 C atmosphere 20 C vacuum of T T T 0 1 2 3 4 5 6 7 8 9 10 Days 100 After 8 years preservation 10 C atmosphere E 10 C vacuum 4 1 C atmosphere 1 C vacuum ial m 20 C atmosphere 20 C vacuum Cotyledon expansion Bb 0 1 2 3 4 5 6 7 8 9 10 Days Fig 5 Germination Rooting and Cotyledon Expansion of Cannabis Seeds Toc Upper Germination within a year after harvest middle rooting after 8 years preservation lower cotyledon expansion after 8 years preservation FIERE TOKE EA CT A yeto HD cm cm day Fig 6 r2 0 83251 0 98518
4. THC 200 24 20 24 Ek 10C 1C 20C 8 25 12 il Het No 2 239 4 HA cm 1f cm day 5 THCA THC
5. Toc 1 1 cm day T 2 7 cm day 1 1 1 7 cm day 3 1 5 cm day 1 3 2 7 cm day 7 1 7 cm day Tochigi field 3 y 2 6877 450 e R 0 89326 Snow White tee Sarr 400 many iy Rl yia 8 y m YUS 6 greenhouse 2 Y7 1696x R 0 97237 300 a THC phytotron 1 pees 8 1 5454 mce LE 200 White Rhino feld 3 2 OE feld y 1 492x arly Bud field 3 bo gsog 100 E Kaya field 3 yarana j i Toc y 1 1208x phytotron 1 R 0 98518 0 0 50 100 150 200 Days Fig 6 Plant Height Elongation Rate cm day of Cannabis Obtained by Linear Regression Slope of linear equation is a rate cm day of cannabis plant Correla
6. 1 Toc td 7 100 94 10 AX 8 7 77 89 58 82 1 1 1 7 cm day 1 5 cm day 1 3 2 7 cm day 1 7cm day DE DE
7. AN VL BERR DS A Purple Star Pur ASS 3 5 mm 2 6mm Hawaiian Gold HawG 5 0mm T Toc 4 1 mm 1 3 Un3 10 4 mg Toc 31 7 mg 5 6 yal 240 Vol 130 2010 Table 1 List of Cannabis Seeds Category Strain be Commercial product Cannabis seeds for bird feed CBDA Toc CBDA To
8. L Fig 1 2 2000 1 2009 3 1 N uw N uw oO Seed weight mg m 0 5 10 15 20 25 Seed length mm x seed width mm Fig 2 Linear Regression of Seed Weight and the Product of Seed Length and Width 3 1 CBDA Toc Fig 2 R 0 85277 Fig 2 Y 1 9922X 12 015 X seed length mm Xseed width mm Y seed weight mg KO HE
9. HawG THC The Doctor Doc Seed length mm PN RR UD Seed width mm O e NOW A u Ice Pur Mas Asa Toc Ots THC Bel Ger Au1 Au2 Un1 Un2 Un3 Sk1 HawG Doc Sk11 2 Sk11 1 Pow HawS NLB Seed weight mg Ots es THC ea Bel oO o Fig 3 Length upper Width middle and Weight lower of Cannabis Seeds Black columns in the seed weight mg figure are the estimated values calculated by the linear equation of seed weight mg and the product of seed length mm and width mm Y 1 9922X 12 015 Fig 2 Pow ice z Sk1 1 2 Mas M NLB E Haws M Pur E Asa eeN N ononown eo To Au1 e Au2 e Un1 1 Sk11 1 M 5 0mm 2 3 5 mm 4 1 23 0 mg DOA THC Ots 4 8mm 4 4 0mm 2
10. 2 3 7 100 94 20C 1C 10 8 3 ARKO 7 77 89 58 82 10C 8 1 8 CERES 7 L
11. R IRT 15 42 tetrahydrocannabinol THC e mail yoshimat nibio go jp 2140 57kg 503kg A
12. THC DOA THC 150 mg 1 THC THC ail 2 7 5 0 mm 2 1 4 1 mm 1 6 7 31 7 mg 4
13. 22 6mg 2 Doc Ots HE HE 1 2 7 0 mm 2 1 4 1 mm 6 7 31 7 mg CHS 1 ORE 2 4 No 2 241 HawS Ice Fig 4 Toc Sk1 Sk11 1 Pow mn D qe SAWD OS Mas NLB Cannabis Seeds Scales represent 1 mm 4 3 Toc Fig 5 1
14. CAM DOA THC 60 C 2 REEK 150mg 1 REFERENCES 1 The Ministry of Health Labor and Welfare Japan The Japanese Pharmacopoeia 15th 246 Vol 130 2010 2 3 4 5 ed 2006 p 1298 The General Measures against Narcotics and Stimulants A buse The Ministry of Health Labor and Wel fare Japan December 2008 Yoshimatsu K lida O Kitazawa T Sekine T Kojoma M Makino Y Kiuchi F Bull Natl Inst Health Sci 122 16 20 2004 Khryanin V N Chailakhyan M K Soviet Agricultural Sciences 7 16 18 1978 Yoshizawa M Aragane M Fukuda T Suzuki Y Kitagawa S Shioda H Yasuda I Ann Rep Tokyo Metr Inst P H 57 127 132 2006 Situation of Administrative 6 7 8 10 De Meijer E P M Bagatta M Carboni A Crucitti P Moliterni V M C Ranalli P Mandolino G Genetics 163 335 346 2003 Rowan M G Fairbairn J W J Pharm Pharmacol 29 491 494 1977 Takashim
15. DOA 11 8 244 Vol 130 2010 REM Abaxial leaf in Mk vegetative growth 1 oe ake A ARIZ RK O BAR FIXCSIX ee 8 8 THY Cannabis flowering k Kenaf vegetative a growth late in August TE Flower Cannabis male foe 8 9 September Hr x Kenaf bisexual i October 10 TE Flower Cannabis female September BS Fruit SS ARORRLBK AR RE 1 Cannabis fruit S i Ae AB TE October A aw vy Cannabis harvesting
16. cannabidiolic acid CBDA CBD K A tetrahydrocannabinolic acid THCA THC 39 THC CBDA THC THCA 7 Old CBDA CBDA THCA 1 Fig 2 Table 1 1 Fig 3 Fig 4 1 Fig 2 k 1 lt
17. Hibiscus cannabinus L 2007 4 18 Ho AK 15 15 15 L 6 15 15 15 6 S DOA THC Ots 150 mg 2ml 60C 2 2ml 10 1 5 ml L 140 DOA Triage Drugs of Abuse Panel Plus TCA Biosite Diagnos tics San Diego CA92121 DOA
18. 1 2000 1 2009 3 31 50 16 32 HE 10 20 6 12 10 10 Fig 1 2008 4 1 2009 3 31 19 12 MI 2 cannabidiol CBD THC
19. Fig 7 PMOL NS No 2 243 HROL Time Germination Vegetative growth Stem in vegetative growth Stem in vegetative growth Petiole in vegetative growth Adaxial leaf in vegetative growth Abaxial leaf in vegetative growth
20. Kenaf flowering f f October Seed 2 HY Fig 7 Continued eal lg s Fig 8 Cannabis Ots Female Flower Bud left inside the red circle and THC Detection in it by Triage Drugs of Abuse Panel Plus TCA center heat dried sample right fresh sample No 2 245 THC REK THCA THC 1g F
21. Adaxial leaf in vegetative growth Fig 7 _ KARR THC Kenaf Characteristics A Rw A J A BR OD SE SSH Lh E COMO MBA NLAR RAR ite FR ewe RC Ak Characteristics of Cannabis THC and Kenaf DOA THC 6
22. DOA THC Biosite Diagnostics REPO THC 11 nor 9 carboxy 42 tetrahydrocanna binol 50 ng ml V CBD 0 1 mg ml THC 1 ug ml cannabinol CBN 2 rg ml THCA THCA THCA THC 3 THCA 2 Al Ots 150 mg Fig 8 left 60 2 THC Fig 8 center THC THC 4
23. tion coefficient r was between 0 83251 and 0 98518 Plant height and culti vation period data were quoted from Yoshimatsu K et al 2004 1 Khryanin V N and Chailakhyan M K 1978 2 and Yoshizawa M et al 2006 3 Field field cultivation sowing date March 31 2003 in 1 April 1 2005 in 3 Phytotron 1 Pot soil leaf mold Kureha compost 3 1 1 cultivation in phytotron 24 C 14 h light 20 C 10 h dark 60 rela tive humidity Greenhouse 2 cultivation in soil filled flats sowing date March 23 1977 18h light 5 KERZ 4 Hibiscus cannabinus L THC L
24. YAKUGAKU ZASSHI 130 2 237 246 2010 2010 The Pharmaceutical Society of Japan 237 1 mi Regular Articles w Characteristics of Cannabis sativa L Seed Morphology Germination and Growth Characteristics and Distinction from Hibiscus cannabinus L Kayo YOSHIMATSU Takashi KITAZAWA Noriaki KAWANO Osamu IIDA and Nobuo KAWAHARA Research Center for Medicinal Plant Resources National Institute of Biomedical Innovation 1 2 Hachimandai Tsukuba Ibaraki 305 0843 Japan Received September 15 2009 Accepted November 11 2009 Published online November 12 2009 Illegal cannabis Cannabis sativa L cultivation is still a social problem worldwide Fifty inquiries on cannabis that Research Center for Medicinal Plant Resources Tsukuba Division received between January 1 2000 and March 31 2009 were itemized in to 8 categories 1 seed identification 2 plant identification 3 indoor cultivation 4 outdoor culti vation 5 germination and growth characteristics 6 expected amount of cannabis products derived from illegal canna bis plant 7 non narcotic cannabis and 8 usage
25. a D Tochigi Prefectural Agricul tural Experiment Station Research Report 28 47 54 1982 Ogata J Kikura Hanajiri R Yoshimatsu K Kiuchi F Goda Y Yakugaku Zasshi 128 1707 1711 2008 Biosite Inc DOA Specificity Table Trade Name http www biosite com products doaSpecificity pdf cited 7 September 2009
26. chigishiro Otsu Ots THCA THC THCA Mexico Seeds of RCMPR Belgium nal Germany Ger 1 Austria 1 Aul 2 Austria 2 Au2 1 Unknown 1 Un1 2 Unknown 2 Un2 3 Unknown 3 Un3 Skunk 1 Sk1 Hawaiian Gold HawG The Doctor Doc Skunk 11 Sk11 1 Seeds in inquiry Power Plant an Hawaiian Snow Haws Ice Cream Ice Skunk 11 Sk11 2 Purple Star Pur Master Kush Mas NL x Big Bud NLB 1 2 7 4 8 mm 2 1 4 0 mm 1 6 7 31 3 mg 2 7 4 4 mm 2 1 3 1 mm 6 7 18 1 mg phones 4 2 4 8 mm 3 4 0 mm 23 4 31 3 mg 5
27. of medicinal cannabis Top three inquiries were 1 seed identification 16 cases 3 indoor cultivation 10 cases and 4 outdoor cultivation 6 cases Characteristics of cannabis namely seed morphology germination and growth characteristics and distinction from kenaf Hibiscus cannabinus L that is frequently misjudged as cannabis were studied to contribute for prevention of illegal cannabis cultivation Key words Cannabis sativa L Hibiscus cannabinus L morphology germination 1 ROM IRO 2007 nl Cannabis sativa L
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