末端標識オリゴヌクレオチドプローブ評価例
Contents
1. PCR ALCOOE ilLuminate the change 1 Terminal deoxynucleotidyl transferase TdT 70mer 3 Q dUTP Q dUTP 1 10 PCR 100pmol LL 1 L 5x Reaction buffer 4 ubL RR 25mM CoCLl 4 LL Labelling ONE 1mM Q dUTP 2 Sitamine durp anddNrp ser TdT 400U gL 1 uuL ALR101 NN Water 8 L te Total 20 gL 137 C 15 0 2M EDTA 2 L DNA dUTP dNTP ALR 101 22. 20 L 1 pg 10X Buffer 10 UL Eo Water 60 huL Labeuling ONE Alkaline phosphatase 8 mg mL 9 HL re hosphatase Labelling Kit TGase 10 U mL ME Ripe oto Total 100 uL se Aloka Medical Ltg 40 C 3 ALR 103 Prehybridization 30 Hybridization 10 ng mL Wash1 30 x 2 Wash2 10 x 2 CDP Star 70 Hybridization buffer 2 M Urea 100 mM Tris HCI pH 9 5 0 5 M NaCl 1 mg mL torula yeast RNA 1 xDenhardt s solution 3 Casein 4 Dextran sulfate MW 500 000 0 1 Triton X 100 Wash1 buffer 2 M Urea 100 mM Tris HCI pH 9 5 150 mM NaCl 0 1 Triton X 100 Wash2 buffer 100 mM NaCl 100 mM Tris HCI pH 9 5 50 mM MgCl 0 1 CHAPS Labeling ONE Triton Dow Chemical Company Hybond GE Healthcare UK Ltd CDP Star Tropix Inc 181 8622
3. 6 22 1 0422 45 5129
4. Labelling ONE Ao4 HITACHI DNA lnspire the Next Labelling ONE DNA 3 RNA mRNA PCR DNA PCR DNA SCR 525nt SCR 70nt 60ng Hybridization buffer 6mL 70 ng Hybridization buffer 7mL total RNA 3 ug 1 2 50C Hybond N CDP Star 70
Download Pdf Manuals
Related Search