様式 C-19 科学研究費補助金研究成果報告書
Contents
1. 2 200 000 660 000 2 860 000 3 1 300 000 390 000 1 690 000 6 6 5 5 5 1 1 W5 ww w wW 6 6 6 6 0 3 500 000 1 050 000 4 550 000 1 scaffold i scaffold2. FGF 4 FGF 1ng FGF 10ng FGF 100ng 4 3 1 2 3 3 FGF 3 5 FGF lng FGF 100ng FGF 10ng 5 3 3 5 FGF 3 3 scaffold 3
3. i rhbFGF 2 rhbFGF 3 FGF 3 1 1 basic FGF FGF Lot No 80HCC 10 mg mL
4. 3 ALP FGF FGF 3 3 FGF 10ng 100ng ATP Ca 3 3 FGF 5 3 1 A Tamura T Ara Y Imamura T Fujii P L Wang The effects of antibiotics on in vitro biofilm model of periodontal disease Eur J Med Res 13 439 445 2008 2 M Kitamura K Nakashima YKowashi TL Fuiii H Shimauchi et al Periodontal Tissue Regeneration Using
5. 100 1000 10000 0 1 v v 100 10 1 ng mL 2 Lot No HF3B 1 3 Lot No 080627 10 M 8 10 mM 172 7 uM 50 mg ml 4 DNA DNA Lot No HDP 5 E Lot No AR987 6 ALP ALP Lot No 825 7 1 n Lot No LTH7700 i Dexamethasone minimum 98 HPLC SIGMA Lot No 096K1805 8B GLYCEROPHOSPHATE Disodium Salt Hydrate SIGMA Lot No 85H5718 iv KOKEN CELLGEN I PC L
6. C 19 C 2007 2008 19592392 21 3 31 Periodontal Iissue Regeneration using the Bone Marrow Mesenchymal Stem Cells with the Calcified issue Conductive Collagen Composite FUJII TAKE0 30173389 3 FGF
7. Fibroblast Growth Factor 2 Randomized Controlled Phase I Clinical Trial ProsOne 3 1 11 2008 3 FUJI lakeo TSUJI Hifumi Antibiotic strategies for the treatment of periodontal disease Clinical Research in Dentistry 5 54 65 2008 2 1 TI Fujil T Lombardi PC Baehni et al Application of Phosphophoryn collagen composite to horizontal bone loss in periodontal defects in vivo 34th European Calcified Tissue Society 2007 2 TI Fujil P L Wang Y Terada H Tsuji PC Baehini Effects of Melatonin on Pro inflammatory Cytokine Production in PGE2 activated Human Gingival Fibroblasts in vitro 35th European Calcified Tissue SOclety 2008 6 1 FUJII TAKEO 30173389 2 SATTO TAKASHT 2 40265070 ABIKO YOSHIHIRO 90260819 SAITO MASATO 50337036
8. PBS 0 5 mL well PBS 0 5 mL 1 mL 80 C 3 30 10 000 rpm 4 C 5 ALP 0 2N HC1 300 UL Ca DNA 3 n 3 4 1 4 1 DNA FGF 1 3 Dunnett 1 DNA ug mL 1w C 1w lng 1w l0ng 1w 100ng 3w C 1 3w ing 3w 10ng 3w 100ng 1 DN
9. A 2 Ca 1 FGF Ca 3 FGF Ca 3 10ng 100ng 3 Dunnett 6 lt 0 05 2 Ca ug mL web 150 100 TT 5 0 a ee 1w C 1w lng 1w 10ng 1w 100ng 3w C 3w lng 3w 10ng 3w 100ng 2 Ca 3 ALP 1 3 ALP FGF 10ng 100ng Dunnett p lt 0 01 3 FGF ALP 3 ALP K A 50 1w C 1w lng 1w l10ng 1w 100ng 3w C 3w lng 3w 10ng 3w 100ng 3 ALP 4 1 1 3 1
10. ot No 118064 2 50v v 3 0X10 cel1ls 0 5mL we11 24 1 5 CO 870 0 5 J 1 1 0 4 10 7 PBS 0 5 mL well PBS 0 5 mL 1 mL 80C 2 3 0 4 7 2 21
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