取扱説明書(ECL-SUN)
Contents
1. TBS T NaCl 100 350 mM Page 14 WESTAR ECL SUN instructions XLS063 11 WESTAR ECL SUN 100mL kit NN WESTAR EtaC WESTAR EtaC Ultra WESTAR Supernova WESTAR ECL SUN 50mL kit WESTAR WESTAR Nova 2011 50mL kit 2011 50mL WESTAR Nova 2011 50mLkit WESTAR Starter Kit WESTAR EtaC 50mL kit XLS025 0000 WESTAR EtaC Ultra 20mL kit WESTAR Supernova 20mL kit MSDS2. 4 TBS T NaCIl 100 350 mM WESTAKR ECL SUN instructions XLS063 Page 13
3. CN ES 2 5 4 C
4. 80 100 V 30 60 15 25 V 20 30 SM xii V R 500mA
5. 15 PVDF 30 15 e 15 kDa 0 45 um e 15kDa 0 2 u lt 15kDa ie PVDF WESTAR ECL SUN instructions XLS063 Page 7 4
6. 4 3 5 TBS T VI al 1 bj cj 23 HRP d SE 2 TBS T 30 1 TB5 T 3 5 4 3 5 4 1 6 WESTAR ECL SUN
7. 15 30 1 5 VI CCD CCD 3 5 1 5 Es A 1
8. WWW Cyanaden Ccom WESTAR ECL SUN instructionsXLS063 Page 15 Le KK SSE gt 103 0001 18 10 Tel 03 3661 4662 Fax 03 3661 0369 http www nmkkk cojp Cyanagen Via degli Stradelli Guelf 40 C 40138 Bologna Italia Tel Fax 39 051 534063 Email info cyanagen com VAT 02360111203 n REA 433637 10 000 00 Finance amp Technology 2497 bis cc VAT 02958121200 6 119 000 00 US7803573 EP1962095 US7855287 EP1950207 US2012009603 A1 CA2742025 EP2405016 sm Page 16 WESTAR ECL SU
9. 4C TBS T NaCl 100 350 mM S5 BSA
10. 30 60 ii 2 ii TBS T 2 TBS T 3 59
11. 1 1 EE Eee _ 4 CCD 1A 8 CCD i WESTAR ECL SUN instructionsXLS063 Page 11 10 5 TBS T Tween 20 0 1 0 59 v v 5
12. TBS T NaCl 100mM 350mM BSA59 6 HRP 2 5 SDS SDS SDS
13. Anode Foam pad F a a Membrane Gel ii 2x Filter Paper 2 V VII Vill X i 2 ix NG Cathode iv
14. 10 9 11 CCD 10 co Ch Xx Ny lt JH NM Page 4 WESTAR ECL SUN instructionsXLS063 1 425 nm lt
15. 1 100 1 5000 1 1000 1 15000 Page 10 WESTAR ECL SUN instructionsXLS063 9 ij ii 1 1 Westar ECL Sun 4 5 i TBS T iv 1cm 0 1ml v 1
16. Cyanagen Mi 100 60 Cyanagen ISO9001 9 EER ER Cert bal ran Page 2 WESTAR ECL SUN instructionsXLS063 WESTAKR ECL SUN WWW CVdriddJeT COITI LO 5 7 WESTAR 2 i 6 3 SDS PAGEY 4 O
17. HRP 0 2 um Page 12 WESTAR ECL SUN instructions XLS063 lt 10 kDa 1Ig
18. BSA WESTAR ECL SUN instructions XLS063 Page 9 8 i D TBS 1 2 II III 103 4 3 5 I CR TBS T iv TBS a v 30 1 TBS T
19. NH HOa NHs Cy HO NH Cr MH Ta NM NM Ok aminophthalate Ns hy HRP enhancer lst 0 Iumminal lurmininl radical ni IrTIGl endopercox de WESTAR HRP WESTAR Kricka L J 2000 Methods Enzymol 305 370 390 Heindl D and Josel H P 1997 Non radioactive Analysis of Biomolecules 258 261 Springer Berlin Marzocchi E Grilli S Della Ciana L Prodi L Roda A and Mirasoli M 2008 Anal Biochem 377 189 194 Vdovenko M M Della Ciana L Sakharov 1 Yu 2009 Anal Biochemistry 392 54 58 Vdovenko M M Della Ciana L Sakharov 1 Yu 2010 Biotechnology Journal 5 8 886 90 Vdovenko M M Zubkov A V Kuznetsova G l Della Ciana L Kuzmina N S Sakharov 1 Yu 2010 mmunol Methods 362 1 2 127 130 CQ LN 4
20. Page 8 WESTAR ECL SUN instructions XLS063 6 5 ic ii iii iv 10 v PVDF TBS T e e e 250ng 7
21. 1 0ml 0 59 S 100ml ImageQuant LAS 4000 Mini GE Healthcare DIAS I SERVA ChemiDocXRS VersaDoc BIO RAD Chemilmager Alpha Innotech Image Station 2000 40000MM Kodak ANNN PANNN Odyssey 1L 10 100mL 1L 1L 10 200mL 1L TBS T 1L 10 TBS 100ml Tween 20 1 mL 1L 5 BSA BSA V 5 g 1 TBS T 100ml 1L 10 S 100m 1L FOTO Analyst Luminary FXSystems Fotodyne LAS 3000 Fujiflm UVIchemi UVIprochemi UVItec Ltd G BOX GeneGnome Syngene FC L COR
22. Page 6 WESTAR ECL SUN instructions XLS063 3 SDS PAGE ii 2 iv v Pre Stained Standard viii 90 130V VI VII 4 45
23. N instructionsXLS063
24. WSTAR a a i WESTAR ECL SUN instructions XLS063 Page 5 2 A B 1L 10x A TRIS 250mM 30 3 g 1 9M 144 0 g SDS 1 w v 100g 1L 1L 10x A TRIS 250mM 30 3 g 1 9M 144 0 9 1L TBS T 1L 10x TRIS HCI 20mM 24 23 g TBS T NaCl 136mM 80 06 g 800mL pH 76 1M NaOH 1L 5 5g 1 TBS T 100ml 100mL 10x
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