3A1-RD1/T11-FITC
Contents
1. No Wash No Wash Q PREP Multi Q Prep TQ Prep Q PREP Multi Q Prep TQ Prep 3A1 CD7 T CD7 T T CD7 CD7 TCR T2. PBS 1 12 T CD7 13 1 10L 1 TQ Prep Multi Q Prep TQ Prep Multi Q Prep 7546999 300 3 A B
3. 4 FS SS FITC RD1 CYTO COMP CYTO COMP CELL T8 FITC T8 RD1
4. CD2 CD7 T11 FITC 3A1 RD1 T NK B AML CD7 3 9 CV 596 51 CD2 y 0 98 x 2 1 r 0 975 CD7 y 1 01x 0 9 r 0 978 CD2 CD7 y 0 99 x 0 3 r 0 977 1 0 196
5. 7 8 9 10 CD7 T T CD2 T CD3 CD5 11 TQ Prep Multi Q Prep
6. 1 3 4 T B NK 3 T B T B T lg E T T Smlg B lg Fc
7. pan T 959 6 T NK CD2 T CD2 B 3A1 RD1 CD7 40kD 3A1 CD7 T T E CD2 85 CD7 NK B T11 SFCI3Pt2H9 CD2 T BALB c NS 1 AG4 3A1 3A1E 12H7 CD7 T HSB BALB cJ NS 1 AG4
8. T T ALL CD34 AML CD7 CD7 T T T11 CD2 T T T DiGeorge T T11 3A1
9. Q PREP PBS 20 25 6 a gt 10 x 10 mm 10 20 x103 2 20 30 x103 3 30 40 x10 5 40 60 x103 6 60 100 x103 10 100 200 x10 20 b 3 x 103 mm3 1 25 C 500 x g 5 2 3 4 LH700
10. T CD7 4 CD2 CD3 CD7 ATL T CD7 NK CD2 CD7 6 AML CD7 CD2 CD7 T 7 B CD2 CD7 T T
11. lg lgG1 H L T11 lgG2b H x L 3A1 T11 FITC FITC Fluorescein isothyocyanate 1 4 FITC 3 10 468 509nm 504 541nm 3A1 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 lt 575nm 568 590nm 1 0 5mL BSA 0 2 0 01M Nacl 0 15M NaN3 0 19 CD2 CD7 2 CD7 T 2 T CD2 CD7 488nm FITC RD1
12. 2 3 4 5 6 1 2 8 C 2 20 25 3
13. 3A1 RD1 T11 FITC 6604239 50 0 5mL 1 Haynes BF 1989 Personal communication 2 Haynes BF Mann DL Hemler ME Schroer JA Shelhamer JH Eisenberth GS Strominger JL Thomas CA Mostowski HS Fauci AS 1980 Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans Proc Natl Acad Sci USA 77 5 2914 3 ReInherz EL and Schlossman SF 1982 Strategies for regulating the human immune response by selective T cell subset manipulation The Potential Role of Cell Populations in Cancer Therapy Feller A ed Raven Press New Yorl p 253 4 Haynes BF Martin ME Kay HH Kurtzberg J I988 Early events in human T cell ontogeny Phenotypic characterization and immunohistologic localization of T cell precursors in early human tetal tlssues J Exp Med 168 1061 5 Haynes BF Denning SM Singer KH Kurtzberg J 1989 Ontogeny of T cell precursors a model for the initial stages or human T cell development Immunol Today 10 3 87 6 Kamoun M Martin PJ Hanson JA Brown MA Sladak AW and Nowinski RC 1981 Identincation of human T lymphocyte surface protein associated wlth the E rosette receptor J Exp Med 153 207 7 Howard FD Ledbetter JA Wong J Bleber CP Stitson EB and Hertzenberg 474 LA 1 981 A human T lymphocyie differentiation marker defined by monoclonal antlbodies tha
14. 10 5 TQ Prep Multi Q Prep PBS 2mL 400 450 xg 5 5 2 PBS 1mL 6 EPICS 7 2 2 2 lt 8C 24 5 1 2 1 2 100wL 3 10L 10L
15. FS Q PREP 0 5mL 3 FITC Log RD1 PE Log 2 RD1 PE FITC CD2 Quadrant2 T11 8A1 Quadrant4 T11 3A1 CD7 Quadrant1 T11 3A1 Quadrant2 T11 3A1 CD7 CD7 3A1 RD1 CD7 Count 186 1088 18 RD
16. 3A1 RD1 T 11 FITC IMMUNO TROL 6607077 Fc MSlgG2b RD1 MslgG1 FITC 1 29 6 Quadrant 1 2 4 29 1 CD2 T CD2 85 CD7 2 T CD2 CD7 3 CD7 T
17. PMT o 1 2 3 EDTA 6 4 9096 5 6
18. 4 45 5 PBS 2 3mL 400 450 xg 5 6 7 PBS 25 1mL 30 2 8 250 L 9 PBS 2mL 10 400 lt 450 x g 5 11 12 7 lt 9 13 0 5 1mL PBS 14 EPICS 1 2 Q PREP EPICS
19. lg T B T B TdT HLA DR Smlg T B T B T pan T CD7 CD2 CD5 CD3 CD3 CD4
20. C 2 1 6603152 300 1mL PBS 24mL 25 9 259 6 3 PBS PBS 6603369 1 500mL pH 7 20 2 4 MslgG2b RD1 MslgG1 FITC 6604238 50 0 5ml EDTA 3 10 x 103 mm 10 x10 mm 3x 10 mm
21. 5 10x10 mm 6 1 100L 1 Q PREP TQ Prep Multi Q Prep 3 A B C TQ Prep Multi Q PREP 1 12mm x 75mm 2 100 L 3 10 L 10L 4
22. 2009 11 2007 2 21100AMY00250000 6604239 877449 T 3A1 RD1 T11 FITC 2 4 ww FITC Fluorescein isothyocyanate RD1 Phycoerythrins PE 1 T11 FITC 0 2 lt 0 6g 3A1 RD1 0 3 lt 1 2g T11 FITC CD2 50kD CD2 E
23. CD8 T Pan T Pan B T B Pan T CD2 CD7 T11 3A1 T T
24. nt pleuripotent lymphohematopoietic stem cells Blood 73 381 16 RelInherz EL and Schlossman SF 1981 The characterlzation and function of human immunoregulatory T lymphocyie subsets Immunol Today 2 69 1 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Sao COULTER 6604239_0911
25. t block roseWe formation J Immunol 126 2117 8 Kensky AM Robbins E Springer TA Burakoff SJ 1984 LFA 1 LFA 2 and LFA 3 antigens are involved in CTL target conjugation J Immunol 132 2180 9 Palacios R and Martinez Maza O 1982 ts the E receptor on human T lymphocyies or negative signal receptor J Immunol 129 2479 10 Meuer SC Hussey RE Fabbi M fox D Acuto O Fitzgerald A Hodgdon JC Protentis JP Shlossman SF and Reinherz EL 1984 An alternative pathway of T cell activatlon a functional role for the 50k T11 sheeperythrocyte receptor protelIn Cell 36 897 Brottler P Boumsell L Gelin C and Bemard A 1985 T cell antivation via CD2 T gp50 molecules accessory cells are required to trigger T cell activation via CD2 D66 plus CD2 9 6 T11 1 epitopes JImmunol 135 1624 12 Sillclano RF Prart JC Schmidt RC Ritz J and Reinherz EL 1985 Activation of cyiotoxic T lymphocyte and natural killer cell function through the T11 sheep erythrocyte binding protein Nature 317 428 13 Haynes BF Metzgar RS Minna JD Bunn PA 1981 Phenotypic characterization of cutaneous T cell lymphoma New Engl J Med 304 22 1319 14 Pittaluga S Raffeld M Lipford EH Cossman J 1986 3A1 CD7 expression precedes T gene rearrangements in precursor T lyrnphoblastic neoplasms Blood 168 1 134 15 Kurlzberg J Waldmann TA Davey MP Bigner SH Moore JO Hershfield MS Haynes BF 1989 CD7 CD4 CD8 acute leukemia a syndrome of maligna
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3A1 RD1/T11 FITC