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1. 000 0 9002. a s Mi gt Sr 101 00 0 00 U 00000 19030 741 00 0000 000000 UUUUUUUUUUUUUUUUUU
3. 900
4. 000 0000 00000000600 0 O majour histocompatibility complex 0O 000000000000 uuagagullilililitullultLtLtL O IL 18 IL 69 TNF a uuuuuuufuiuultututLLutLt 2999 uagdadduuututltutLuttt taut B B amyloid precursor protein T1 0 00000 MAP kinase 00000000000000 5 rm TNFa 0000
5. Presep C short CHsCN Sm 2 CHsOH 5m H gt O 10m 1 000m4 1 000500000009 CHsCN HPLC 10u 4 3m 1 1mol HCI g pH3 50 O O O O O OR N T TI D E E E E B C E EE UI I 1ng 1 000m42 1 000 Blank 0 31 g 0 5 10 15 20 25 30 HPLC conditions Sample 1 Estrig E3 Column Wakopak Navi C18 5 4 6x150mm 20176 estradidl E2 Eluent 5 NaClO 3 Ethynylestradig 2 2 6 lt 41 34 66 4 Estrofi Flow rate 1 0m4 min at 35 Detection ECD 900mV RANGE 1 ATTEN 5 Injection vol 1002 DU 1000 TAERE Long 000000000000 LOU 3 44 2003 00 n jd 5 20 25 per ecc 5 10 15 20 25 293 41951 Presep C Long J O IO 110 29 000 297 41851 RPP Short 000000 10 xj 39 000 Oe 100 0
6. 0 No 000001 0000000000 p 0000000 133 13611 1019 mnn 135 14391 M CSF 0 165 21141 182 01471 1419 ocola 000 00 000 000 184 01791 O00 000 000 0000 0000 184 01671 RAN D 0 0000000000000 0000 LESS S PISTE 303 09721 MOH UR 032 15671 1001g 155 0557 0000 sis 39 000 148 000 39 000 39 000 37 000 39 000 37 000 10 500 36 500 032 17751 00000000000000 0 7 000 017 18441 DDDDDDRANUUUODO g 3500 013 18921 LI E D E OL EI E UE LI C 5029 35 000 026 14811 000002 00 000 0 2 35 000 023 14941 0 0 0 00 1400 000 0 rnBMP14D 39 000 044 28621 100000 000 6m D 150p rfl 241 36 000 041 2631 24 39 000 306 09711 DU0D DU 1D D HD DO D 000001 90000 30108600 000001 D CUOBO1D 100 000 Vol 71 No 40 20030 Bd BE
7. mi 11 36 RANKLO E UDO U U U 36U 100 0 000000 Burgess T L et al J Cell Biol 145 527 538 1999 10 000000000000 00000000000000 00000 Pyk2 Proline rich tyrosine phalloidine 000000 BU UU U Pyk2
8. 0000000 ROH R COOR 10 methyl RT methyl ethyl propyl butyl 20 acryl ethyl salicyl RI methyl 40 phthal terephthal octyl 2 ethylhexyl 5 methacryl methyl 0 60 heptyl methyl gt 1 Temp 130 C Methanol Methacrylic Acid 3 mol mol LHSV Methacrylic Acid Conv 99 Methyl Methacrylate Select gt 99 X z COY 8 X X X RIO J R Tm ri h ZJ C 2 YO Z0 CH U 40 XU CI XT H YO Cl 40 pili yc zj x 2 Catalyst Yield SO4 ZrOe 31 7 WO ZrO z 10 5 SIOz Al Os lt 0 1 Reaction condition p chlorobenzoyl chloride 20mmol Chlorobenzene 200mmol Catalyst 2 09 Temp 135 C Time 3h Vol 71 No 40 20030 DX otes 2 2 DMB 3 Catalyst Conversiofi wt 2 2 DMB 2 wt Pt SO4 ZrOIE Pellet 27 5 Pt SO ZrO Powder 845 15 6 Reaction condition Temp 200 C Press 1 08 Mpa LHSV 1 5 ht 5mol mol MeOH flow rate 1 5g h 9 flow rate 100m4 min 9 Column WO3 ZrOs Pellet 1 0g CH3OH 200966 C
9. 0 0 00 00000000 19371 1000 000000000060 III IU L 00 0 0 19630 0 0 0 001960 100000 0 0 280 0000000000000 J ELO 000000000000 O0 Bia LB diim UU UUUUUUUUUU
10. UJU SIII L OUOU CHOO OUUU 0000 00 00000 00000000000 UUUUUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUUUU ra 0 I L 0 00000000 0 0 UUUUUUUUUUUUUUUOUUUU 0 UUUUUUUUUUUUUUUU LIU l 0 O U UOUN UUO UUU UUO L DU U U U U UI D 00000 00 00000 2000000
11. 7 Vol 71 No 40 20030 0 0 Benveniste E N Am J Physiol 263 C1 1997 Dong Y and Benveniste Glia 36 180 20011 Aloisi F Glia 36165 20011 Golde T E Nat Med 8 938 2002 Weiner H L and Selkoe DJ Nature 420 879 20021 Akama K T Albanese C Pestell R G and Van Eldik LJ Proc Natl A cad Sci U SA 95 579 1998 McDonald D R Bamberger Combs and Landreth GE J Neurosci 18 4491 1998 Nomura J Hosoi T Y and Nomura Y Life Sci 72 2111 2003 Nakagawa Zhu H Morishima N Li E Xu J Yankner and Yuan J Nature 403 98 2000 101 Borovikova LV Ivanova 5 Zhang Y ang H Botchkina G I Watkins L R Wang H Abumrad N Eaton J W and Tracey KJ Nature 405 458 20001 11 Wang H Yu M Ochani M A mella
12. 4500700 00000 Uu UD D D D 2 5ppmi 0000000000000 L BL LU LL 000000000000 5 0 290000 T T TT DON DON
13. 0 UU i uu LL http www amano enzymecojp Lipases for Resolution NHCOCHs gt a NH 20mM Tris HCI pH7 5 30 41 1 0M N Ac D L Phe BHANG COOH COOH N Ac L Phe p e HPLC Of D phenylalanine with D amino acylase D Phe N Ac L Phe N Ac D Phe 6716 D Pne 8546 and A symmetric Synthesi 1995 53 668 1995 32 1076 1996 000 00000 59 96 20010 qI 00 00000000600 39 2001 e0 0000000000 37 627 2004 000 000000600 76 1098 2002 91000000 00 0 6F 14 2003 Ema T et al Bull Chem Soc Jpn 71 443 1998 Kazlauskas R J et al J Org Chem 56 2658 1991 J Org Chem 60 6959 1995 Biocatalysis 9 209 1994 1 LE LI D LL WOO 78926 A1 Moriguchi M et al Biosci Biotech and Biochem 59 2115 1995 C amp EN p 48 May 5 2003
14. pH30 5 0 1201 00000000000 Ut 21 0 0 L UUUUUUUUUUUUUUUD U L 000000 BO 000 0 EUM HO 0 oH OH CH2OH 5 y x OH OH 0 0 0 0 aibi 0 HEBLYI 0 40 OAOE E
15. 0 1993 j 2000 LH DD HH BL L RANKLDUT TD DL O 0 0 0 0 OUL 5 1 Src homology2 domain containing tyrosine phosphatase 11 1 0 U SHP 1
16. 000000000 000000000 19651 0000 00L 000000 00000 0000000 UUUUUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUUUUU 240 000000 0 19661 1977100 0 0 0 uuum 0 0 0 0 0 3000 000 0 L 50003000000 00 19600 n O00000 13000 L 141 150 0000000 L oggi uiui 0 0 19691110000 0 0000 19951 0000000000 340
17. 000 000 0000000 n O D O 00000001 633 04491 RA100010 AgraQuant DON 48 1 D 0 I 42 000 632 04503 RA100020 AgraQuant DON 96 DONT 80 000 AgraQuant Romer Labs Inc 00000 Vol 71 No 40 20030 Q Products 0 ULTRA 20 200 0 00000 0 0 0 0 00 NIHO 20 20 0 000000 Protein Function Identification Layered Membranes 0 150 Membrane 1 Membrane 2 Membrane 3 Membrane 4 Membrane 5
18. 4010 2423 0000000 0 0000 0000 00000000000 ere Xe ee wi e NS e Nee A sh Anse 2 e e 500 1000 1500 2000 Auvo 0 e uv o000000 UVsterilized and lyophilized 0 100 000000000000000 0 8 36 000 Talking of 0 93 SLPDUTDLD UL E BL EB Ra 5 E 8 EH E EB BEES B LE BETTER ELTE LIT LE A Ej EB B 1998 Langevelde Staphylococcus aureus ELE RATE TE PI
19. MK j MK Q0 0 0 Up 0 0000 000000 00000000 gt d d d 0 0 050505 d lt gt d d lt gt 0000 uuu MGP 00 0 0000000000 OO tutt 9 0000 000000000000 enam 000000000 D uan III d Km guai UUUUUUUUUUUUUUUD UUUUU KA MK 7J DD UU 0 MK 40 0 0 00000 00000000 1 DUBKIMK ADDDMK 000000 0 000000000 0 410 000 00000000 0 0 Zhang Y M Li X Y Sugawara H Koyama Biochemistry 36 14638 14643 1999 Booth SL Suttie W J Nutr 128 785 788 1998 Sokall J J Booth S L O Brien Davidson KW T saioun Sadowski A
20. 0 CH3 z Su 3 O H CH3 CH3 ugggugnpggdguopue O CH3 O H CH3 n 4 13 00000800000 05 05 lt gt lt gt lt gt gt lt gt 005 0000 000 0 0000000000 VIII O 0 X0 0 OO U proteins C S 21 Q SI U D D z0 UU 0000 0000000 00000 00000 LU B D EULBIULDDULDIEULLDLULBLLL 0 0 000000000000 Had Haan growth arrest specific O00 00000000 0 uuu O OKO uauuuauliuliull KL Uelull O Gag U 000 00 0000000000000 coch 0000 U ucocr
21. 0 0 0 0 Bam 00000 PSI 5 000000 354 0000000 0 0 0 HDH HEH H HNH 5 UUO UHUU 0 PSO Candida O 0000 p AYS DODDU 0000000000 UUDUDUDUDUUUDUD S DUUDUDDUD D 000000000000 00000 0000000000000 UUUUUUUUUUUU UA spergillugl Bacillus 00000000000 U UUUUUUUUUUUUUUUUUUU 0 0 Vol 71
22. No 0 0 nn 905 9919 23 000 575 3269 31 000 9578 32881 26 000 28 000 Products SuperSep 0 0 0 0 005 50 000 000006060 5050 000000 00 0505 05058 d ud D D 3 L3 3 L3 3 C3 3 L3 3 C3 o 12345678 9101112 pana 200 000 116 248 bet m IE m 66 267 EE 42 400 30 000 x T 17 000 M 10 000 UU SuperSep 5 20 12well O No 194 12961 Buffer Sold x 2 2 Me fll 196 11022 Buffer 5011 1000 O O No 184 01291 L L Quick CBB Dl 00 299 50101 UU U 00 1 2 78 Lane3 4 9 10 E D UD D Lane5 6 11 12 EL L D E E D U 150000000 SuperSep 0 O00 0O00 0 150 000000 000000 12100 0 0 0 11001 0000000
23. 100 000000 00000000 10074 DB l 0 0 0000 OO DOOL a 00000 00000000000 0 90000000 000 0000 00 0 NH CH2 Si NHCH CHsO e Particle size 1601 m Surface area 40m g SiH il 5 Si O 0 672 Vol 71 No 40 20030 Days 0 00 0 ggg 0000 00 000000000 UUUUUUDLUUUUUUUUUUUU 0 0 HPLC I Ti O 0 0 0 0 8 3440 00000
24. 180 0 FGF aFGF uU 00000 0 0005 OO1ng m g O 0 0 BALB c 3T3A310 I ED 0 01 0 05ng m g n D td D BB uttiae4g nmvEDs 20001 0005 nv BALB c 0000000000 0 ED 0 005 001ng m Hueco 00000000000 Vol 71 No 40 20030 Products FGF bFGF 10 J E coli q E coli EDs 03 14 0 ED O5ng U U U U J X 1 units mol U 0 0 0 FGF bFGF
25. O O 210000 uauuuiuuuuulutlututtlutl 000000000 000000000 L MAP kinase NF B De BN N Nitric oxide Endoplasmic reticulum Caspase Apoptosis Pro inflammatory cytokines LPSOO 20 a 9 0
26. T EL 06 6203 3743 0 URL http www wako chem coj p E E ER RR RR RT mun E mail oonishi reiko owako chem coj p ETE ETE TELE RI TELE BE E ELE T 0120 052 099 120 052 806 E mail labchem tec wako chem co j p S N En S d
27. D OH T ous S0 O O l UUUUUUUUUUUUUUUUUD 9000 00000 0000000000000 0 iux mie wis wie iw iw wie ie iw ie wie wie wie tie wie wie Bie wie iu iu iwi wie wie wie wie l wie wie iiie OWako o D BN on 162 20931 Partially Hydrolyzed Saponin Standard mo EDS T FE T E Vol 71 No 40 20030 35 000 TLD E m mam gne O OOO Oa
28. 05110000 000000000 6000 00 0 0 0 0 0 0 Vol 71 No 40 20030 000000000 0000000000 0000 000000 0000006060 000000000000 07711000 000000 000 00000006060 0 0 28 00000 00060 000000000000000 19697 upggnmnggnnugnnangnnn 19710 00 0 0 0 19A 0 0 0 0000780 0000000000 O I 198810 15 0O000 000000000000000 0000000000000 000006060 O00000 0000002000000 900 0000000000000000 0 000000 000 00 0000600 00000000 19700 19881 0000 000000 01972 750 00000 0 000 019770 780 0000600 1000000000 9770 08000060 00000 0000019820 np 000060 A 280 000 6 OUUU L
29. amp 000000 UDUDUDUUDDUUUUUUUU D PP h2 S IHA 4 Isopropyloxazolin 2 yl ferrocenyl diphenylphosphine 481 35 dis CAS No 163169 10 6 1mol RuCIP PPha ip CH3 PhoSiH2 1mol AgOTf Et20 0 degree OH nA LL 42 yield 90 NH2 1mol RuCIp PPha ip irr i Ph2SiH2 mel AgOTf THEE 45 yield 74 e 04 0 5mol PPha ip FOXAP gu iPrONa H j NE iPrOH rt 94 yield 99 60 04 uuu Nishibayashi Y and Uemura S Synlett 1 79 1995 Nishibayashi I Uemura S and Hidai M Organometallics 17 344 1998 1 Nishibayashi Y Arikawa Y and Uemura S Organometallics 18 2271 1999 qd 1 Nishibayashi Y Ishii Y Mizobe Y Uemura S and Hidai M Chem Commun 22 2360 2001 Nishibayashi Y akei 1 Uemura S and Hidai M Organoratallics 18 2294 19994 Chung K G Miyake Y and Uemura S J Chem Soc Perkin T rans 1 2723 2000 00 ou n o 065 04331 100mg 11 000 FOXAP 061 HUUU 500mg 44000 OUUU Presep C RPP U U
30. 0 0 0 0 FigAm 000000 0 0 US 0000000 e e E ananas P syringae X X campestris 3000 Fig 1 HD U BEBE EL B EIO 7I DE OL D U EE DI 3 5 9 D D 9 8 9 9 9 0 80 0 J MM nun 630 04641 00000 FH UVH D Dui Vol 71 No 40 20030 45 0677 v Fig 2 HU DLL OF Nucleation Active Bacteria UV sterilized and lyophilized from Erwinia ananas 20001 0000 ugumuuiuiull tduaum x 00 UD EIL
31. absorbance fluorescence 400 450 500 550 600 650 700 750 800 wavelength REARS HL Wars Eb unn n m n mn 046 28681 DY 630 NHS 33 000 in vitro PBS kit 0 RINA GmbH in vitro PBS Protein Biosynthesis Systems 300p g mA Ne mRNA ribosomes K ribosomal factors tRNAs synthetases amino acids energyl 0 0 g m LU uut mRNA PCR plasmid HOU transcription in vitro PBS kit Prop S Mik 0 0 D L1 L1 D 350 x 50 T Mi amp Buffer 0 300p x 10 300p x 20 1 6001 2x 1 000
32. po 10 agg 00000000000 U 19291 ilt Vol 71 No 40 20030 d 0 diii D Opp pulp 19911 197140 0000 00 0 1934 0220 0 340 0000000 L 0 0 000 000000000 10010 00000000 00000 00000 O 000 000 0 000000000000
33. 0 130000 Vol 71 No 40 20030 Hunter Cell 80 225238 1995 S et al Cell 111 305 311 2002 Takayanagi H et al Nature 416 744 749 2002 Lam J et al J Clin Invest 106 1481 1488 2000 Azuma Y et al J Biol Chem 275 4858 4864 20001 Kobayashi et al J Exp Med 191 275 286 20001 Kong Y Y et al Nature 402 304 309 1993 Takayanagi H et al Arthritis Rheum 43 259 269 2000 0 Takayanagi H et al Nature 408 600 605 20001 101 Umeda S et al Am J Pathol 155 223 233 1999 Aoki K et al Bone 25 261 207 1999 12 Takeshita S et al Nature Medicine 8 943 948 20021 131 Sims et al J Bone Miner Res 14 clear zone ruffled bordel 0 00000 S183 1999 14 Sanjay et al J Cell Biol 152 181 195 2001
34. 361 2210000 0000000000000 0000000 1929 0 0 0000000000 10000000 uud 19700 00000000000 1939 00 00000000060 400000 ieu 19541000000 oggi UU 0
35. 0 0 0 0 0000000000 194310000 D pu 000000 1978 1n 120 D UD U UU 0000 UU 0000 0 2959 0 000000000 1971 10000000000 00060 Ba 00 000 0000 2000000000009 0000 0 000 0 0 00000 00 00000000 OOOD DU 0 UUW Barm 00000 0 0 0 III U L O OOL UUUUUUUUUUUUUUUUUUU 00 0000000 830 00000060 199318 00 0000000000000 Vol 71 No 40 20030 os00o0000 0 LB LLL Georgin
36. SHIP Src homology 2 containing inositol 5 phosphatase 5 5 src H H LB Ej BETTE 0 00 0 10001 000000000000 00 0 Fig 1 Fig 3 0 5 Src homology2 domain containing tyrosine phosphatase 10 0000
37. HxCDF 0 7 23 44 595 167 0 74 HpCDF D74 2 3 3 4 4 5 1560 0 74 120 OCDF 1 04 2 3 3 4 4 5 1570 0 74 3 4 4 5 TeCB 810 0 74 23 344 5 5 HpCB 189 0 74 Utt 10 2vol 20 50vol Fig 11 3vol CHsCl Hexane 20 50vol Fig 2 IU DM T 10 5vol CHsClz Hexane 20 50vol Fig 3 VLU 10 Hexane 20 5vol 50vol CH sClz Hexane Fig 40 00 ELLE ES ES ES p p 6 6 Q Q 9 Fig 1 I O V J U J PCDD DrFs IH OU DH O U 3vol O 0 5vol 100m2 0 0 O O mono ortho Co PCBsII 0000 PCDD DFs 50vol CHs Cl Hexane LH H EH E B dgutyeietzi ettet t 0 non ortho Co 81 126 169 5vol CH Cl Hexane 000000000 0 00 D 0000000 298 42551 Presep 0 000 000000 51 16 000 gH uu 00000000 291 41751 Presep Cylinder 13 000 D l 45 000 X Fig 2 Dunne 500 150m 635 04191 Luer Stopc
38. oco REUS oos RED a E FEES cc EE eso SEX sea EE 0009008 E seo SE gue MN on nuunnnmn 290 34251 Presep C DNPH Vol 71 No 40 20030 29 000 90 0 echnical Report
39. 0 E coli 0 E cali 00 EDs 50 100pg D EDs O5ng mf 00000 x 1Cunits mgr O 0 U 1 3 lt 000000000 3000000000 D0000000000000 0 S Um Eco GDDDDDDDDDA7UDDDDDDD 00 EDs 50 100pg me D BALB c 3r3A30000000000000000 0 EDs 05 m4 00000 x 1 units moll 2 4 U 00 Eco pguggdguugteuuuua du U EDx O5ng m LI U U U U x 1 units 0 0 2 ED O5ng md j U U U J x 1 units mol U 0 ggagnggaggdaun J Eco 1900000000 J 00 ED O5ng men 00000 10 p Ecai 1 00000000000000 J 6 2000000000 J E coli J E ol O0 BDHED O5ng md 00000 x 1 units mo gogog UB EDs 10ng D D x 10units mgl 1 d OMNI
40. 0 370 2040 D unm I OOOO Bp D g B HOO 1956 135500 0000 00000000000 100100000000 000000 0 0000 100000800 0000000000 0O00 0000 uit 082 0 2 0000000000 D BOB 195610 D D 00000000 oggi 196241 Bu 1954 1980 00000000000 0 0 0000000000 1964 0 000000 000000060 D OUBBBBL 000 0 00000000100
41. BETE ERR S ELE BH D P e n m ET EE Bio Esse BIO EB HL Bi EBERT ELI EIER EPI I Ip FEET o m EET GI Hl p Bacterial Translocation Pp B ER o Tabata E S ES 20 E EST ES
42. RANKL receptor activator of NF K B ligand 0000000000000 0 0 c Fosh utl Vol 71 No 40 20030 08 0 uguali bi p oU U UUUUUUUUUUUUUUUUUU
43. 1 0 SDS PAGE 12345678 9101112 ava me E _ 66 300 _ 55 400 36 500 2559 31000 21 500 _ 6 000 15 12well O O O No 194 13061 Buffer 5011 x 2 2 00 No 196 11022 Buffer 501 1000 O O No 184 01291 JL Quick CBB 0 00 299 50101 0010 1 HIST IRI ERAI 75 100 9 1251 9 150 9 5 200 910 200 9 200 000 150 000 100 000 50 000 10 000 0 o o ma nan 192 1290 12 000 1902911 Supesep 75 50 DDDD 10 14 000 19612921 SuperSep 100 0000010 12 000 195 2991 Supersep 10 wel SE 0000010 14 000 190 12941 SuperSep 12 5 ue 50 T 10 12 000 IPE 8 12 000 ens ex T CED Sese Tel 0 1 jon 18000 194 12961 5 5 2 10 12 000 191 12971 SupeGsp 5201 0000 10 12 000 0612081 0 __ 10 12 000 195 12991 10200 Ter __ 10 12 000 Vol 71 No 40 20030 Products 100 No Ud 0000000 196 11022 Sample Buffer Solr x 2 2 Me 3 100 193 11032 Sample Buffer Sol 2 2 0
44. Human 00000 29 9 39 000 092 05081 Human 10000 10 9 39 000 095 0507 1 Interleukin 17E Human recombinant 39 000 098 0506 1 Interleukin 17F Human recombinant 39 000 Interleukin 13 Vari H 091 05051 nterleukin 13 Variant Human 10 9 39 000 recombinant 002 05101 Peredo Furan contra D00000 lg 39 000 90 05111 0 uran cont 000000 1049 39 000 96 051271 22 Furan recovorant 000000 101g 39 000 014 19671 ATRL Human recombinant 000000 29 39 000 058 07201 EGEGF Human 0000001 10 9 38 000 00 0 0 0000 duni 0 00 0 00 201 00000000 IU U 30 352 0000 000000000 FGF10 0
45. J Clin 65 779 784 1997 Kaneki M Hedges SJ Hosoi Fujiwara S Lyons A Crean 5 et al Nutrition 17 315 321 2001 Yamaguchi M Uchiyama 5 Tsukamoto Y Int J Mol Med 10 729 733 2002 Schurgers L J Dissel P E P Spronk H M H Soute M Dhore C R Cleutjens Vermeer C Z Kardiol S3 111 57 111 63 2001 Schurgers L Vermeer Haemostasis 299 20001 2 o eJ za HP Goo umn u n as J nu mnunmn 13314291 Sanda TID DDD DD DDD awe Vol 71 No 40 20030 20 000 0 Quillaia Saponaria Molina acid 00 0000000000 000 UUUUUUUUUUU UD
46. Products 000000000000 000000 IL 171 IL 1 O00000 FUIL 0 31 100000 0 0 LU uui biu utili uuu 000 000000000 Interleukin 17 0000000 E coli EDx 2ng 000000000000000000000 461000 Interleukin 17D 0000000 E coli 0 000060 Interleukin 17E ED 1 E E E J E coli 0010 100590 IL 17E I D L L IL 8 Interleukin 17F 0000000 E coli 0 000060 IL 13 Variant U DD D U 1 121 U 000000000000 A2010 Dd WB 7 7 U 7 D 5 113 0000000000000 Interleukin 19 0000000 E coli 0 Interleukin 20 0000000 E coli 0 5 Interleukin 22 ll 1 D E E E E coli 0 Activation induced TNFR member ligand J E coli 10 10 0 9 AITRLO O O O0 0O IL 8 0000000 Endocrine gland derived vascular endothelial growth J E coli 0 00000 pu gr 1EU p g0 0 Ta D opm 0989 0509
47. x Products uuulliiuiiliL UU RNase 0 D D D RT PCRE DNAD OU DNAD O RNase 0 0 Protease UUUUUUUUUUUUUUUUUUUUUUUUUUUUDU Tren Fia 1175 D gga r ei U 1 RNase Protease O O 0 RNase 0 0 25ng gt P 2unit O 14 160 00000 0 0 200 0 0 0 50unit O 0O O 147 18 00000000 7q 101 0 DNasel cloned plasmid expressed in Pichia uuu uu ju uy 0000000 548 02331 Deoxyribonuclease Bovine recombinant 1 000U 7 000 544 02333 Solutio RNase free Protease free m 10 000U 30 000 Bet SAAITI U U ll 0 000 LU CRP DDUDDUD SAAD SAAN TT 0 0 0 181 DU 750
48. C A Tanovic M Susarla S Li J H Wang H Yang H Ulloa L AI A bed Y Czura CJ and Tracey KJ Nature 421 2003 121 Watkins L R Maier S F and Goehler L E Life Sci 57 101 1995 13 Hosoi Okuma Y and Nomura Y A m J Physiol Regul Integr Comp Physiol 279 R141 2000 14 Wang H Y Lee D H D Andrea MR Peterson Shank R P and Reitz A B J Biol Chem 275 5628 20001 AS T are m ase ave ave I ye 191 000000 BE HB O 1000150 110 2800 0 10 00 17 00 n aoo Me 3215220000 100r 0 D Uu 100050 0000 00 00000 4nononn c oooooooooorooooooor nn nuan 101 500 00000 110 300 00 00 121 100 2 000000000000000 190 190 131 500 3000000 140 300 252 00000000000 00 00000 150 100 00000000 sooo 151 300 oo nn 00000 190 190 gn nn 190 500 00000 uon nnon nn EL Br 0 O 42010000 EU E E ELE E 102 0023 LH D LE EL U DE EE UE DE CE UE DE D SC 13 FAX
49. 042 2902 5 800 DEREN 5 500 arsit TT onate 1 m EE Solution Sodium Undecylbenzenesulfonate 192 13121 Standard Solution 7 500 Img mi Methanol Solution CED 199 13131 Standard Solution DODDUDDDU Sodium Dodecylbenzenesulfonate 174 5 7 500 mg m Methanol Solution n onate 11 die ml Met m oolution Sodium Tetradecylbenzenesulfonate 193 13151 Standard Solution 7 500 1mg m Methanol Solution 1 U nn nun 081 041751 Fepeeyere 00001 19 5000 122 04351 em oolution 10 5 15 700 Sodium linear K 3 Dodecylbenzenesulfonate Standard ABS dc 194 0805 1 Sodium Dodecyl Sulfate Standard D 0 0 0 2 500 CYWako B n D e n u 0 gd 00000000 1 Chlorodecane Standard Solution 034 19031 1mg m Hexane Solution 5A 5 000 CYWako 2000000020 00200 No In n 0000000 p Bromofluorobenzene F uor GED 029 15021 Mixture Standard Solution 174 5 5 500 each tmglm Methanol Solution Fluorobenzene Standard Solution CEM 069 04471 mof Methanol 00000 Doon msa 5 000 p Bromofluorobenzene Standard Solution mg ml Methanol Solution EBERT
50. gt 9 9494944499444 gg e e e e e e e Ue lase UD 0 No wn ooon o nunnnnmu aia esmo poemon emsan os et m R a Lipase F AP15 Rhizopus oryzae 150 000units g 7 0 40 Lipase G Amano 50 50 000units g 1 5 0 40 in E Burkhold ao i 5 Burkholderi 7 zm E 7 E eee mmy emen p m om mim pese omena omeen em 22 Vol 71 No 40 20030 OOOO Km i pp 0 00000 Kg Kim d OUUD III BD UUU nMK nn 2 np M K 4 MK H HL 00 JULI III I U U 0OOO uu 0 0000 000 0 0 0 00 casi 0000000 D 150 D 0 0 KO 0000000000000 U etum 00000 BEIDE Y 0 000 000 0000000000 etai U
51. kDa 21271 SA 12 12 l 2 12 12 48 kami 000 1 4 20 P FILM 500000 6512048 Lane 1 20u HaCaT cell lysate Lane 2 20u g Jurkatt cell lysate Membrane 1 00000000 20 3U 40 000 0 Membrane 50 Membrane 10 50 uuu Vol 71 No 40 20030 GS 1202 GS 1204 GS2031 GS2032 GS2033 Membrane stack 1 10 Stack 2x 5 Stack 1x 3 Stack 2x 3 Stack 3x 3 Stack Transfer Buffer 5 200m 10 20 10 20 3 Reaction Folder Protein Transfer Control 1L 20 10 20 S Product Manual 1L 1L 1L 10 10 x1 Reaction Folder 0000000 200000000000 2 Protein Transfer ContrQ PTC ODA OU CU UTOR ODEON OOU OHU U HOH No 0 J00 26 000 28 000 10 000 15 000 22 000 25 E S gt 102 GS 1003 GS 1004 Membrane stack 2x m x 2xb D Transfer Buffer 5X l Reaction Folder CoverEasy Square SERERE EE Labeling pn Product Manual 10 x Cover Easy Plastic Square
52. No 40 20030 1000000 COOH oo000 UU ULE EEL UUUUUUUUUUUUUUUUUUU 0000000 00 10mg 1m4 D 0000000 0000 00000000000 0 D UD UO 0 OU DC L TECO 50 0 00000000000 UUUUUUUUUUUUUUUUUUOU I II 0000000 0 0 0 50 0000 0170040 0000000000 10 0 0000 5 00 OUUU DOU OUL 0 B ID ftp i D EB gi D LI hi U L D UU D l
53. p minii O00000 Vol 71 No 40 20030 De E IE IHE E E HE Hi Bic ETE EE CIE LE B LEE HII LE ABIRE Ee E BE ELE 0 0 00000000 0000060 B UE ODDO RTT RT EI EHE Lc ED ELO SE EE S ge E ES Hn ICI CAI DONE EE MMU Tsuchiya M et al FEMS Immunol Med Microbiol 15 129 134 19968 65 4114 1997 Langevelde P V et al Antimicrob A gents Chemother 42 3073 3078 1998 Tabata et al J Gastroenterol 37 726 731 2002 E LN 2 20 EET Products Wako SO Zro WO Zro
54. 1500 LEAS HRAL a qm Lot 0000 2 BENE usd zm 4 aH Avidin HRP 100p well 50 m 1001 well SAA Biotin 0 J O 50 well 100 well v 450 O 620nmp CD ABS 450 620nm 1 10 100 1 000 10 000 ng m LPS L T D D DU DU U ng m4 Fo REL E DRE 0 17 g H J I 1 0 5u g H 0 2 4 6 8 10 14 16 18 20 22 24 12 pgg 0000000 0100 630 03421 AKRSA 010 Lbis SAA Mouse 55 000 Vol 71 40 20030 Products OWako DY 630 NHS 633n5m 000000 635 5 Ug CaoH49NsOsS 2 731 90 Ex 630 nm Em 655 nm 1211 concanalvalin 201 antibodies U U 120000 DMF 5 U U DY 630 NHSO
55. m min T emp 40 Detection UV 360nm Inj Vd 102 90 0 2m 24m0 4 6m 1 27 O O 3 C 0 J gt CO C t cO c Fig 2 200000000 DNPH 0 DNPH BI DNPH 0 00A 25 0000000 910 DIO 00 0000 00000 00000000 971 9910000 00000 00000010 ming 00 99 0 0 Presep C 0000000 00 0 1 27 25 D D 0 D D 0 B OOo O C Fig 3 3m4 min 27 G O 25 BO Oo Ce Ce Fig 4 5m2 min 27 0 0 25
56. 0000 3 100 184 01291 Running Buffer x 100 5 200 299 50101 Quick CBB 9 000 299 13841 Silver Stain Kit 9 000 291 50301 Silver Stain ll Kit Wako 9 000 299 58901 Silver Stain MS Kit 19 000 293 57701 Negative Gel Stain MS Kit 11 000 910 5 UUUUUUU U U U 100 100 3 mm A2 SuperSep 5 2094 0 0 20 O O O 00 750 0 0 TIT 14 10 00000000000000000000 1800000 0 130 00000000000 1700000000000 Supersep D D D 1000001700 25 OSU 000 0000000 TrichromRanger Prestained Plate S 0 35401 26691 prot
57. 00000 800 2 10 1300 2x 10 440 2x 10 Control Vector 100nM 15u x 1 RNase free water 1m x 2 EFTsI D D U EH 2 U 0 30u 4 0 D U 100 0 0 0000000000 Vol 71 No 40 20030 Products 5 1058 ex 10 1050 2x50 350p x 100 B Mi amp Buffer 000 70g 2x 1 330p 2x 20 1 050p 2x20 A Mik N CI OOOO Oj 35u 2x 10 170p 2x 10 1 050u 2x 1 000000000 2x10 130p x 10 850p x 10 Control Vector f 1 00 15u x 20 RNase free water 1m x Stable Isotope DBDDBBDLEFTSD D UD lU 32kDa 20 D 00 10000 SI in vitro PBS NMR 0 0 0 nnn Prep Cloning vectors of pIX family pix 1 0 p aras bos inicia Le Brei M Bii T22 sawan Pawa TIZH er lt X Wb 115P 115 A E 1 MI 2126 pas e TD n M Paa 1116 in vitro PBS CBB di 4 9kDa TAIHuUgagudsiliiutitiutitlutLutlttl 48kDa uuuuluiuuuiutiulliilil JU BE OB OE EEB ETE D mRNA RNase 50 DO00000000000 0 6kDa BEATLES HL DL
58. 03 3270 8582 TEL 03 3270 8243 E mail seminar 9 wako chem co jp Vol 71 No 40 20030 DNPH _ DD 00000060 QOU OUOO0 U U0 gud bump umm 0 Vol 71 No p D D UO B B UUUUUUUUUUUUUULUUUUU UUUUUU UUUUUUUUUUUUUUUUU UU UUUUUUUUUUUUUUUUUUU 0000000000000 251 g 0 dada bp bru 27 Fig 1 j4 DNPHJO 000000 01010 0 0000 1500000 0000000000000 Hun auda uada minl 25 0 m minl 0090 0 1041011 bNPHI LH U DU HPL dg 0000 0 H E 1444 0 14 minx 24h T Column Wakosil 5C18 RB 4 250mm Eluent CH CN H20 60 40 Flow Rate 1
59. 294 36851 resep eomma 0000001010 25 000 290 36951 Presep lT 10 32 500 290 37051 RPP 500 000000110 37 500 Vol 71 No 40 20030 Products A AS xn Fig 3 A dq K nsn 50 lt o lt i xv v 4v 4v CO O a5 50m2 Li AS CN m50 150m 00 PCDD DFsSII U UO B GC MS 9 2 ag Su Fig 4 0000 5 16 Tablet 000000010 Unit pg Columnf 0310 0321 Dioxins reagent blank Dioxins reagent blank CH TeCDD 0 51 3 3 4 4 TeCB 771 0 74 PeCDD 0 54 3 3 4 4 5 PeCB 1260 0 74 HxCDD 0 74 3 3 44 5 5 169 0 74 PCDD DF HpCDD 0 74 2 3 4 4 5 PeCB 123 0 74 00000 e e 3 3 4 4 CET T I 5 1 T sm ni PeCDF 0 54 2 3 4 4 5 PeCB 1140 0 74 DOO O O Hexane1oom
60. DEREN J DD Ecol U DU FD 10ng mv 0 D000000000000000 H 3 No061 02851 aFGF from Bovine 5 00000000 01 9 90 1EU u D D 04006102851 O 000000000 00 1 DD Ecol No 067 02831 100EU mg 1 05 00000 ggg Qu B 0 00000000 1 M dum I 200 067 02881 Bovine recombinant 149 34 000 067 04031 bFGF Human recombinant 0 0 0 0 19 500 uuuluiutulsiuttutL utl 069 04091 Rat recombinant 0 0 0 0 19 500 E coli 062 04341 FGF4 Human recombinant 39 000 05 00000 x 10unit mgr J J J i 069 04351 FGFS Human recombinant 000000 39 000 066 04361 FGF6 Human recombinant 100001 39 000 10000000000000 119 00661 Human recombinant J 0 34 000 063 04371 FGF8 Human recombinant 39 000 L Uu D 9 uult 060 04381 9 Human recombinant 2019 39 000 0 067 04391 9 Mouse recombinant 000000 1019 39 000 0 EDs 05n
61. Ez DL t 6 t RBS ATG sto gt D s 5 5 enhancer coding sequence U 000 0 0 du pnm 57 5071 109 01 ConngvecosofpXfamlg tugu 1019 38 400 mUDUD No o D 00000000 T Mix without Leu and Met Leu and solution separately D in vitro PBSktOOO0000000000000 572 75021 5132 01 0000000 lset 28 800 MW 25mM Met 0 1m l H kDa for E coli promoters without Leu and Met Leu and Met solution Fatty acid binding PSU 579 75031 P 5252 01 separately l5 promoter set 28 800 Green fluorescent protein GFP UDDUDDDUD 0 5mf Chloramphenicol acetyltransferasB CATH 1 Dihydrofolate reductase DHFH 215 5 20 Amino acid Mix 25mM each Single chain antibody T Pes 200000000000 R RJP antibacterial protein 86 D 18 Amino acid Mix 25mM each without Leu and Met Leu and Met EOE 571 75111 P 6302 01 solution separately 25mM each 1set 38 400 Aminoaoyl tRNA synthetase Ly 120 C Leu S Netf 0 D RIO D B Glucuronidase 68 5 nd 000000 Set of 20 amino acids each 50mM 5 8 75121 P 631 1 01 gannnennnnnnpDnn 20 5014 57 000 000 000001 O H 00000000 s 25434 P g 34 04 Flu
62. H3OCH3 DME DMH MeOH ISO bMB DME MeOH 401 isobutane 5 J39 Cs C11 alkylates xylenes styrene s gt phenylxylylethane 1 octene 1 decene B dimer trimer 9 T heptane jet fuel P s CO CO2 OH O o UIUUUDDUUDUUUDUUUDUUUUDUDUUUDUUDU 300 5009121 LB LL C lj Matsuzawa K Am Chem Soc Div Pet Chem 42 734 1997 21 Hatakeyama K Suzuka T and Yamane 34 28 1991 A 00 000000 68 318 1994 00 n n i O 269 01471 _ 5g 7 000 7 Sulfated 15 000 268 01762 25g 7 000 7 Sulfated Pellet 260 017e1 cona 19 000 267 01771 7 000 59 Z T tat 265 01772 10 17 000 262 01782 Zirconia Tungstate 259 8 500 LE ET ET ET L 264 01781 Pellet 100g 25 000 Products Wako ip FOXAP Ferrocenyl OXAzolinyl Phosphinel OMI TD UO D Ra V I D D 0000 89 H III D L
63. ISSN 1347 4804 CHsO e SiH 32 2 Tn pec NHCH 5 n CHsO e Lipase PS C Amano I BH uuusubluiiiliillilititL tL em 2 mM 10 OO000000000 Uu eee 7 000000000000000 H cem 12 nmmnnmn m 5 F B H F HR 6 Talking of LAL SS o PH U TIE 14 BR 28 B n A0000 000000 5 25 uwi s 4 FARINE Ooo sss DT d E T toutes 15 0000 IDE OLD E T 16 2020 Ys630 NHIS zs i ES OOT 2105 5 19 RINA in vitro PBS 26 MIENNE B 6 20 lI 12 TED TETTE TTE 13 oT 16 a 22 a 24 illul 17 24 UO D UBL LO EET EE B EE eee il IM 0
64. UUUUUUUOUD Vol 71 No 40 20030 O00000 UDUUDUUDUUDUU 0 0 0 00 0 00000 2710 0000000 ibi UUUUUUUUUUUUUUUUUUU UUUUOUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUUUU 1090 0 194410 0000000 0 0 1940 0 0 0 I III LIU L 00000 19491 DH DD 0000000 1947 285 1943 000000000000 037 00 0 0 0
65. UUUUUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUDU UUUUUUUUUUUUUUUUUUU UUUUUUUUUUUUUUUUUUUU OUO DUUN OOOHO OEO H D 0 00 UUUUUUUUUUUUUUULUDUU 0 6 OOOO Lipase PS C4LI 0 0000000 TU U uggagagauggugumauau Lipase PS 0 0 OO0000000000000 000 600 0 150 1009000000000 675K qr U 2 Lipase 5 OH LipasePSC 1 1me 20 41 0000 H HH H 15 Lipase PS C I 60 O O Conversiofi N 02 S O O SS lI 2383S 8r9 011 12 8 0 30 O UO U Lipase PS C I 0 0 0000000 00000 800 00 UU UU
66. a Ferry Dorothy Hodgkin A Life Granta Books London 1998 Brenda Swann and Francis A prahamian ed D Bernal A Life in Science and Politics Verso London 1999 M Rayner Canham Women in Chemistry Chemical Heritage F oundation 1998 D C Hodgkin T he crystal structure of the hexacarboxylic acid derived from Nature 1955 176 328 8 D C Hodgkin X rays and the structure of insulin British Medical J 1971 4 447 51 D00000000100000000000000000000000000000000WHo00000000000000 a o ETE ETOILE THE SEL IBI 00 00o 0000000 GED 639 0447 1 Turbidity Standard Solutio polystyrene i00 100m 15 000 mn EN UDUNo 0 0 D D 00000000 Formazine Standard 061 01631 Solutiof 4000 3 300 206 06701 Turbidity Standard 00000 100m 2 900 100m 2 900 Solutio 1000 Turbidity Standard OWako Solution 1000 o O 00000000 Rr A Dioxane sess 1mg m Methanol Solution UE STI 2 25 1 4 Standard 00000 ImixsA 1mg m Methanol Solution 200 09021 CED 049 28791
67. ein Molecular Marker MX 48tubes plate 524 79741 26681 BlueRanger Prestained Protein Molecular Marker 520 79743 26685 M 077 35411 Q amp A ASUUUUUUUUUUUUUUUUUUUUUUUU Ae Supersep 97 0 5 0 A8 Vol 71 No 40 20030
68. g mel 00000 x 10unit mg 000 060 04401 FGF10 Human recombinant 000000 39 000 83300000000000 067 04411 FGF16Human recombinant D D 39 000 064 04421 FGF17 Human recombinant D D 39 000 061 04431 FGF18 Human recombinant 101000 39 000 068 04441 FGF19 Human recombinant 100000 39 000 065 04451 FGF20 Human recombinant UJU D 39 000 Vol 71 No 40 20030 Products OWako UUDDDUUUUUUDUUDI Anti Iba1 Rabbit 1700910 1 T BSI D mg md baig 1 U mmunocytochemistry Westernblotting 2U g m 051 g mt rat primary mixed culture cells D B B Ibat D Imai Y I bata l Ito D Ohsawa K and Kohsaka S Biochem Biophys Res Commun 224 855 1998 Ito D Imai Y Ohsawa K Nakajima K Fukuuchi Y and Kohsaka S Brai
69. n Res Mol Brain Res 57 1998 Ohsawa K Y Kanazawa H Sasaki Y and Kohsaka S J Sci 113 3073 20001 Sasaki Y Ohsawa K Kanazawa H Kohsaka S and Imai Y Biochem Biophys Res Commun 286 294 2001 Kanazawa H Ohsawa K Sasaki Y Kohsaka S and Imai Y J Biol Chem 277 20028 2002 inue nn o of 00000000 llilmmunocytochemistry 019 19741 Anti Iba1 Rabbit 00000 5040 10014 30 000 Westernblotting 016 20001 Anti Ibat Rabbit 0 0 J 508 1044 30 000 DNA D B gg Anti 5 Methylcytosine Monoclonal Antibody gt L BSA 0 19 0 U Westernblotting 1000 0 10000 Immunofluorescence 100 Sano H et al Biochim Biophys A cta 951 151 1988 Steward N et al J Biol Chem 277 3774 2002 No alm 00000000 Anti 5 Methylcytosine Suse SUN 55 Antibody uuu lt AL 0 ALDAPI 5 000000000 O C merge 000000 Vol 71 No 40 20030 E 4
70. ock 101 19 200 Vol 71 No 40 20030 Products 00 0 0 o ELSAD 00 boNIT 0521001 150 00 180 0 0 150 0 0 1662 CORPORATION Veratox For DON 5 5 Veratox For DON 5510 000000000 ELISA eom feto 155 t 2 s amp ap 171000 38 0 D DU L D L o1ppm 25 25ppm Ol OU U U U U 0 025 05 1 3ppm 10 000000 controls samples 0 000 100090000 8331 VeratoefrDON55 DON 80 000 AgraQuant DONI 0 Labsi AgraQuant 000 0000000000000
71. oro Phenylalanin 28 800 578 75001 P 1102 01 in vitro PBS kit trial size 28 800 i U U U RI HI IR E TDD i 574 75003 1102 02 in vitro PBS kit trial size Il 20 0 3 57 000 572 75004 P 1102 03 n vitro PBS kit trial size II 77 000 G cu u D 576 75041 1211 02 15kDa protein control vector 100nM 5924 38 400 in Vitro PBS kit production MTP size 576 75002 1102 14 nosnnnnnnnnnnnnnDnnn 41 230 000 573 75051 T 1201 02 32kDa protein control vector 100M 554 38 400 ZW 570 75061 1221 02 98kDa protein control vector 100nM 5914 38 400 NL mill SI in vitro PBS kit screening size 577 75093 2101 05 c ND D 397 qx 100 000 pooo 0 Ki Optimisation components RNase free 575 75094 P 2101 20 2771085 kit preparative size r xm 450 000 575 75011 6201 010 m 00000000000000 15 C ND D D NVR D 50mM EDTA pH 8 8 50mMIT Vol 71 No 40 20030 UD 28 800 395 EL EL ET EJ ET EJ ET E p 1910 5 12 1994 7 29 LU L LL 000000000
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