二次元電気泳動法を用いた表層タンパク質分離技術の開発
Contents
1.2. 1
3. 49 No4 2014
4. 1 2001 2 O Farrell PH J Biol Chem 250 4007 1975 3 No 35 p 39 2007 4 No 1 p 83 2011 5 No 3 p 33 2013 6 H Connell W Agace P Klemm M Schembri S Marild C Svanbore Proc Natl Acad Sci USA 93 9827 1996 50
5. ing oo oi 0 2 1 B PER BugBuster Y PER Y PER untested untested untested kDa kDa 110 110 80 80 60 so 50 I 50 40 Ee a 40 30 30 Fr 20 20
6. ME GI
7. 1 45 No4 2014 a 1 0 s 2 2 1 LB 10
8. 201 200V 1 400V 16 800V 1 2 3 2 2 3 3 SDS SDS PAGE SDS SDS 17 SDS 1 40mA 46 2 4
9. 48 S 8 y 1 0095x 12308 R 0 9893 B II 10UUOLUU 15UDCODO A 596 5 109 10 159 15 2096 20 2596 25 3096 CV 9 2 4 CV 1 A B 2 A B C3 CV 4
10. 2
11. 9
12. 47 Noy4 2014 3 2 1 SDS PAGE 3 3 60 2 4 1 3
13. SDS CBB Stain One 6 ELA 5100 2 5 Multi Gauge Ver 3 0 Intensity 3 1 eno B PER Y PER B PER Y PER Y PER
14. B PER Y PER BugBustor Y PER Y PER kDa jf 110 et _ 80 1 ES 60 50 IN gt We 20 2 1 Cell Surface Protein Isolation Kit 2
15. 2 099 3 CV 5 250 CV 5 10 28 3 CV 10 15 25 0 CV 15 20 18 3 CV 20 25 3 3 CV 25 30 0 95 CV 20 CV 25 4 3
16. 05 1 0 37 MRS DIFCO 37C YPD 20 1 0 2 0 30C 3 PBS 0 15M 01M pH 7 2 2 2 Cell Surface Protein Isolation Kit Thermo SCIENTIFIC B PER Bacterial Protein Extraction Reagent Thermo SCIENTIEIC Y PER Yeast Protein Extraction Reagent Thermo SCIENTIFIC B PER Y PER Y PER B PER Y PER BugBustor BugBustor Protein Extraction Reagent
17. MERCK MILLIPORE Y PER CBB Cell Surface Protein Isolation Kit C 2 3 2 3 1
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