NASBA Amplification キット
Contents
1. 2 ee 2011 12 2 2011 10 1 2 1 2 2 3 NASBA 20C 70C 4 5 3 1 22. 20C 12 NASBA 55 uL x 10 NASBA NASBA 60 uLx 10 Amplification NASBA 30 pL x 10 NASBA 6uLx 1 1 J Compton Nature 350 91 92 1991 2 41 13 1798 1801 1997 3 2 105 110 1998 113 0033 2 38 18 03 3816 4480 FAX03 3816 6544 Life Sciences Advanced Technologies Inc Ks 113 0033 2 38 18 03 3816 4485
3. B actin NASBA Amplification total RNA 1 1 RNA 1 P1 2 P2 2 P1 5 T7 T7 5 AATTCTAATACGACTCACTATAGGGAG 2 RNase free water TBI0250 2A155 1 NASBA NASBA NASBA 1 NASBA 55 uL vortex 4C 1 20C 1 2 2 3 NASBA
4. RNA 1 2 4 NASBA NASBA dNTPs NTPs NASBA NASBA AMV RT RNase H T7 RNA polymerase B actin forward and reverse NASBA RNA 2 3 AMV RT RNaseH T7 RNA polymerase RNA P1 P1 5 T7 RNA polymerase P2 P2 P1 cDNA P1 RNA AMV reverse transcriptase AMV RT P1 cDNA RNA DNA RNA RNaseH
5. 2 2 DNA RNA 3 3 T7 RNA P1 AMV RT LLLLLLLLLILLLILILILLI RNADNA RNase H cDNA 2 DNA zz Pl 1 1 RNA 2 RNA RNA RNA EXTRAGEN I 3 RNA NASBA total RNA 0 1 ug 2 1 RNase RNA 2 3 NASBA
6. cDNA 1 1 cDNA P2 P2 AMV RT DNA polymerase T7 RNA polymerase 2 DNA T7 RNA polymerase P2 5 RNA RNA 1 RNA RNaseH RNA P2 cDNA RNase H RNA DNA 1 cDNA 3 PD1 AMV RT 2 DNA T7 RNA RNA RNA 2 1 RNA RNA
7. 10 pL RNA P1 P2 0 4 uimolL vortex NASBA 1 60 uL 0 4 jimoL 4 NASBA NASBA RNase free water 30 uL 10 vortex 1 10 uL 200 uL 2 41C 65C 3 4 NASBA 1 0 5 mL 10 pL RNA
8. 5 uuL 10 2 65C 5 65C 3 41C 5 NASBA 5 pL 4 5 4 41C 90 NASBA 5 NASBA 5 NASBA 20C 1 1
9. TBI0250 2A155 2011 12 2 2011 10 1 NASBA Amplification NASBA Nucleic Acid Sequence Based Amplification RNA RNA in vitro NASBA PCR PCR RT PCR RNA NASBA 1 2 1 2 3 4 RNA
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