DTM-101
Contents
1. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order _lifescience toyobo jp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order lifescience toyobo jp gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 42. gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 6 1 p53 2 9 kb DNA 50 ng NED p53 2 9 kb Quick Taq HS DyeMix 3 rt 4 Quick Taq HS DyeMix DNA 50ng 50 gt M 1kb Ladder 94C 2min 1 Quick Taq HS DyeMix 94 C 30sec 2 rTaq DNA polymerase 60C 30 sec 40 cycles 3 rTaq DNA polymerase 68C 3min 4 A Taq Master Mix Forward Primer AATGGATGATTTGATGCTGTCCC Reverse Primer ATAAGAGCTCCCAAGACTTAG 0 2 M 2 PCR
3. TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 3 7 5 a 4 DNA DNA DNA No 2 5 I 8 lt TA gt TAK 101 TArget Clone lt DNAfragment gt NPG MagExtractor PCR amp Gel Clean up
4. 1 Quick Taq HS DyeMix 2 DTM 101 DTM 101X10 2x Quick Taq HS DyeMix 1 25ml x 2 DTM 101 x10 50l 100 3 4 20 2 gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13
5. PCR total 50 pl 2 GC 22 35mer Tm gt 60 C 3 DNA PCR DNA 5 PCR 1 PCR BPB PCR 2 PCR TA TA TArget Clone Code No TAK 101 TA PCR TA
6. 500 bp pTA2 DH5 vw PCR Pe PCR OK PCR yy gt PCR kA Master mix M 100 bp Ladder 1 lt gt 2 a 3 94C 2min 4 94C 30 sec 5 62 C 30sec 25 cycles N 68 C 1 min M 100 bp Ladder Forward Primer CGCCAGGGTTTTCCCAGTCACGAC Reverse Primer AGCGGATAACAATTTCACACAGGAAAC 0 2 M gt TOYOBO
7. 00 17 00 E mail tech_osaka toyobo jp A4152K URLI http www toyobo co jp bio 1 3 PCR 1 PCR Quick Taq HS DyeMix Autoclaved distlled water X 2x Quick Tag HS DyeMix 25 Hl 1 x 10 pmol ul Primer 1 1 0 HI 0 2 uM 10 pmol ul Primer 2 1 0 HI 0 2 uM Genomic DNA 200 ng 50 ul Template DNA Y HI Plasmid DNA 50ng 50 Hl Total 50 Hl PCR 3 2 Predenature 94 C 2min Predenature 94 C 2min Denature 94 C 30sec 25 40 Denature 94 C 30sec 25 40 Annealind Tm 5 ooo Cycles Extension 68C 1min kb cycles Extension 68 C 1min kb Extension 1kb 1min Tm 73 3 4 PCR 1 thin wal
8. 12 11 Code No DTM 101 Code No DTM 101X10 20C Quick Taq HS DyeMix Taq DNA polymerase 2x DNA PCR Taq PCR 2x DNA BPB PCR PCR rTaq DNA polymerase PCR PCR Taq DNA polymerase 30 4 3
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