取扱説明書 - 検査・診断試薬（非医薬品）
Contents
1. http papilio ab a u tokyo ac jp cps hospital 1 1 1 TEL 03 5841 0567 E mail byoin todaiagri jp 13 5 A PPV PPV2. 1 gt iD 100000 ppm 1 ii 70 iv 1 v
3. A A 240 260 nm 350 370 nm UV 320 nm A B 14 6
4. TEL 076 451 6548 TEL 03 5977 5573 FAX 076 451 6547 FAX 03 5977 5574 E mail support nippongene analysis com E mail feast egenome cs jp fujitsu com URL http nippongene analysis com URL http genome e mp jp Copyright 2012 NIPPON GENE CO LTD All Rights Reserved
5. 60 1 2 UV gt
6. MSDS http nippongene analysis com 8 9 LAMP LAMP 10 e Genome Order e Genome Order
7. http www nippongenematerial com 2 48 NE0071 192 TAKA NE0073 BRZ E PE Le 1 n 48 x 4 Spa 20G GEJO KE 200 GEF 49 192 48 192 48 192 20 C 6
8. 17 PPV PPV SA PPV 20 C 1 x 3 1 5 ml 2 0 m 1 x 3 lt
9. LAMP RNA DNA DNA cDNA DNA RNA plum pox virus
10. E 1 lt ia LAMP DNA PPV LE
11. NE0051 4 7 1 1 A 8 1 24 1 re 207 0 ul 575 0 ul 1 2 1 25 0 ul AA 25 0 yi i 3 4 5 20 0 yl 6 63C 60 7 80C 2 8 UV L L 0 n 6
12. 4 5 6 PPV 7
13. PPV B 1 C EDTA D A
14. 2009 3 plum pox virus 2010 LAMP Loop mediated Isothermal Amplification LAMP DNA
15. 2010 1 LAMP plum pox virus PPV LAMP DNA cDNA DNA RT LAMP RNA PPV PPV 63 C 60
16. PPV E PPV LAMP PPV E 1
17. ai plum pox virus Detection Kit version 5 0 0 NEO0 1 NEO0 3 DD version 5 0 0 1 LAMP 2 3 2 6
18. DNA RNA cDNA DNA RT LAMP LAMP Eiken GENOME SITE http Iloopamp eiken cojp amp amp ISO 13485 2003
19. MSDS 3 0 5 10 ul 10 100 ul 200 1 000 ul 1 5 m 2 0 mi 63 C KANMI EDENA PA TEORA ark AFYA T 0 2 m UV 240 260 nm 350 370 nm 1 5 m
20. 20 C PPV PPV RNA RNA
21. 63 C 60 UV 0 80C 2 i i UV aa a y UV 240 260 nm 350 370 nm 320 nm UV a f
22. E VFN
23. 1 le 17 18 19 20 DD 2012 11 NIPPON GENE
24. E PPV PPV PPV E 10 1 20 30C 17
25. 20 0 uU ii 20 0 ul i PPV 1 0 ul 20 0 ul 1 1 1 x 3
26. 1 Maejima K Hoshi H Hashimoto M Himeno M Kawanishi T Komatsu K Yamaji Y Hamamoto H Namba S 2010 First report of plum pox virus infecting Japanese apricot Prunus mume Sieb et Zucc in Japan J Gen Plant Pathol 16 3 229 2 Maejima K Himeno M Komatsu K Takinami Y Hashimoto M Takahashi S Yamaji Y Oshima K Namba S 2011 Molecular epidemiology of plum pox virus in Japan Phytopathology 101 5 567 3 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA Nucleic Acids Res 28 12 e63 4 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechniques 12 3 358 15 7 PPV 1 0 25 0 ul 1 DNA 63 C 60 PPV PPV 1 0 ul 1x 10 16
27. http genome e mp jp i iia 1 LAMP Loop mediated Isothermal Amplification 2 Sn 2 3 SANE MAANA E a ATATIWA AAA WAITAKA ea AKAA a ena 3 4 IAA Wa 5 5 AAA OE r a E r 7 AA WI AA AAA 9 5 14 6 aT DO E A EEA AT ESE E waa 15 7 Ka Aaa O PEE E EE SAE E PEE S AN AN wa Kia kaaa RR 16 PPV LAMP LAMP LAMP plum pox virus
28. 1 2 5 7 17 20 0 pl i
29. gt PPV 23 0 ul 207 0 pi 575 0 pi 250n 25 0 pi 225 0 pi 625 0 pi 1 1 PPV 25 0 ul 10 BO
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