pLVSIN-EF1α Hyg Vector
Contents
1. ee ion 8 DNA v201007Da TaKaRa 7s Tel 077 543 6116 bx 00784319792. Lot No ON pLVSIN EF1 a Hyg Vector SIN Clontech Lenti X HTX Packaging System 631247 631249 631251 VSV G pLVSIN EF1 a Hyg Vector EF1 g PGK mRNA WPRE woodchuck hepatitis virus posttranscriptional regulatory element cPPT central polypurine
3. U3 MCS Pook WPRE aie MCS Smi l Swa l Not Sse8387 Xbal BamH CCTGCAGGAT TT AAAT TCT AGAGCGGCCGCGGAT CC GGACGTCCTAAATT TAAGAT CTCGCCGGCGCCT AGG Form 10mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column Chain length 8626bp Purity 1 Confirmed to maintain the promoter region and Cloning Sites by dideoxy sequencing method 2 Shown to be cleaved at a single site by C a and at two sites by Sa 1 NOTICE TO PURCHASER LIMITED LICENSE K11 cPPT Element This product and its use are the subject of U S Pat No 6 682 907 and pending foreign patent applications licensed to Clontech The purchase of this product conveys to the buyer the non transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer whether the buyer is an academic or for profit entity The buyer cannot disclose information sell or other wise transfer this product its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its compo nents for any commercial purposes If the buyer is not willing to accept the limitations of this limited use statement Clontech is willing to accept return of the product with a full refund For information on purchasing a license to the DNA Flap technology for purposes other than research contact the Transfer of Te
4. tract 10mM Tris HCl pH8 0 1mM EDTA OR 8 626 bp 1 dideoxy 2 Cg1 1 HPR Sal 2 pLVSIN EF1 a Hyg Vector pUC ori 3 cPPT CTS Pitia 3 LTR A U3 MCS WPRE vate MCS Smi Swa l Not Sse8387 Xba BamH CCTGCAGGAT TT AAAT TCT AGAGCGGCCGCGGAT CC GGACGTCCTAAA TT TAAGAT CTCGCCGGCGCCT AGG cee eer ie Ne ee cee 16 1 P2
5. chnology Office Institut Pasteur 28 rue du Docteur Roux 75 724 Paris Cedex 15 www pasteur fr Note This product is intended to be used for research purpose only They are not to be used for drug or diagnostic purposes nor are they intended for human use They shall not to be used products as food cosmetics or utensils etc Takara products may not be resold or transfered modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please call at 81 77 543 7247 or contact from our website at www takara bio com Users must contact TAKARA when they plan to use this product for purposes other than research use The viral supernatants produced by lentivirus vector could contain potentially hazardous recombinant virus depending on an insert gene Due caution must be exercised in this production and han dling of recombinant lentivirus Please follow the guideline for experiments using recombinant DNA issued by the relevant authori ties and the safety committee of your organization or your country in using this product Takara is not liable for any accident or damage caused by the use of this product Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201007Da pLVSIN EF1 a Hyg Vector Code No 6185 Shipping at 20 C Size 20 ug Store at 20 C 2
6. pLVSIN EF1 a Hyg Vector Code No 6185 Size 20 ug Shipping at 20 C Store at 20 C 2years from date of receipt under proper storage conditions Lot No Concentration 9 ul Volume yl Regarding protocol please refer to the product manual supplied with this vector Description pLVSIN EF1 a Hyg Vector is a SIN type lentiviral vector plasmid The production of VSV G pseudotyped or ecotropic lentivirus using this vector together with Clontech s Lenti X HTX Packaging System Cat 631247 631249 631251 allow you to express your gene of interest in virtually any mammalian cell type including primary cells and nondividing cells Expression of your gene is driven by the constitutively active human elongation factor 1a promoter located just upstream of the multiple cloning site MCS This vector contains a hygromycin resistance gene under the control of the PGK promoter for the selection of stable transductants In addition pLVSIN EF1 a Vector contains WPRE woodchuck hepatitis virus posttranscriptional regulatory element that stabilizes mRNA cPPT central polypurine tract element that increases nuclear importation of the viral genome and recombination efficiency into the chromosome of target cells and so on necessary to improve the viral titer transgene expression and overall vector function Vector map for pLVSIN EF1 a Hyg Vector SLTR pgs pUC ori pLVSIN EF1 a Hyg E cPPT CTS Pret a 3 LTR A
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