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Contents
1. Mupid 2Plus 300 ml 2 DNA 2 3 DNA 13 DNA 3 5 7 N NN 3 DNA DNA TAB2. 4 C 2 37 C 1 lxTAE 40 25 ml 975 ml 40 Mupid 2 Plus 300 ml 2 a 300 ml 1 4 g 1 200 ml
3. b a 3 0 C 2 3 200 ml 8 4 1 4 a 0 7 g 100 ml
4. Feel so Bio FAX 3 8277 so4 gt gt gt DNA PCR DNA DNA DNA DNA gt gt Feel so Bio TEL 03 6277 8041 FAX 03 6277 8042 Mail order leaveanest com
5. 4 1 DNA DNA 2 2 1 2
6. 20Q Haemophilus influenzae Rd ind IILI 20C 20C 37C DNA jind III DNA 20 10 1 10 gt Tris HC1 6mM MgCl2 6mM NaCl 100mM DTT 1mM 37C pH 7 5 DNA RNA 20 DNA DNA Hi
7. Feel so Eco TEL 03 6277 8041 FAX 03 6277 8042 Mail ed kitQleaveanest com FAX FAX 160 0004 2 11 6 VARCA 10F TEL 03 6277 8041 FAX 03 6277 8042 a Nest URL http www leaveanest com
8. 4 DNA DNA gt Mupid 2 Plus 100 20 FPP NN 6 1 2 3 DNA
9. DNA DNA base pair bp Loading buffer DNA DNA DNA TT ww 2 CPT TTTTTTTTTTTTTTTTTTTTT TAE DNA DNA
10. 40 EE 4 1 2 1 1 DNA 20 ul HindlII 3 3 ul 3 ul 20 1 1 1 5ml 2 BA 21ul DNA 10 ul DNA 20 ml 1 1 1 40 300ml 40 1 2 8 2 Mupid 2 Plus 8 2 1
11. 4 1 2 1 DNA 110 pl Hind IL 20 ul 20 ul 20 ul MG 110 pl DNA 60 ul 40 25 ml 2g 50 1 1 975 ml 500 g 20 ul 1 5 1 1 2 2 37 1 1 LED DNA DNA DNA DNA TE
12. DNA DNA DNA 2 DNA DNA DNA DNA DNA
13. DNA 0 7 DNA bp 0 6 1 000 20 000 3 na NN es a a 2 0 100 2 000 3 7zd IIT DNA 7 ind III DNA AAGCTT DNA DNA 7 zd IT 8 DNA 6 2 DNA 0 7 6
14. bp base pare 2 HindlII indIII 23 130 bp 8 502 bp 9 416 bp 6 557 bp 4 361 bp 2 322 bp 2 027 bp 4 100 DNA ind III 100 6 DNA 1 6 2 3 5 4 AAGCTT zd III eR AATT ACGT AGCT ATAT CATG CCGG CGCG CTAG GATC GCGC GGCC GTAC TATA TCGA TGCA A Hind Ill Pel Agel Mul Spel Bglll i Ag Ca l A Ssp 4el Stlul Seal A Tr a Agil C 6 Me Acol Xmal Awl Eagl BsiWI Xho Ce Aoe 0 W Pmll Pl Sa 0 6 Sac ll P C _ 6 Pi GC EcoR AgoMIV BssH Nhel BamHl Kasl PspOM
15. EtBr DNA 2 DNA Mupid Blue DNA DNA TAE TBE TAE bp DNA TBE DNA DNA DNA DNA DNA
16. Ne DNA DNA DNA DNA DNA DNA DNA DNA DNA DNA EtBr Mupid Blue
17. 4 3 5 2 ind IT 1 1 ind III 20C 6 37 C 30 60 1
18. 2 3 1 4 2 6 DNA LED
19. 4 6 8 DNA DNA 2 DNA nd III Hind III AATTGAIAGCTTCCGGG 2 DNA RNA DNA
20. 1 17 1 1 3 1 3 DNA 0 10 1 1 37 2 DNA 3 1 DNA 8 2
21. 3 4 1 DNA DNA Haemophilus influenzae Rd Hind IT DNA DNA
22. Feel so Bio 004 DNA 1 100 004 1 2 2 3 4 5 DNA 012 13 14 i 18 19 20 DNA DNA DNA Feel so Bio 00S IDNA
23. I Acc651 Sgl l ApaLl 6 9 Agr G Eg136 EcoRV Nae 56 BstZ171 6 _C 6 _C Aa Sacl Sph Bbel Apal Al A BspH gspEI 0al Bcll BsrG 0 BBI T A SnaBl Nru Fspl Mcl Pi T A 2 5 9 8 1 2 1 200 20 1 10 200 83 20 8 3 1 2 1 2
24. Pa i Nn 0 1 3
25. ndIII EcoRI DNA DNA 20 C DNA 20 C 0 DNA DNA 40 40 TAE EDTA 2 DNA 4
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