Tracer 第49号 目 次 - 名古屋大学アイソトープ総合センター
Contents
1. Oo2. I 3 RI RI 18 RI
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5. 464 8602 HP http Www ric nagoya u ac jp licence TEL 789 2565 FAX 789 2567 _TEL 2565 FAX 2567 TEL 85 2565 FAX 85 2567 9 00 12 00 13 00 16 30 Vol 49 2011 21 II X I 1 X 90 X 91 8 5 2 i ee
6. 50 60 30
7. Tracer PomA B Na 1 gt HH Na
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9. Dk Vol 49 2011 3 PomA PomB Na 2 PomA PomB PomA PomB
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11. 2 600 800 650 Vol 49 2011 1 4 2 70 170 15 2305 RI
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22. J 20 30 40 Vol 49 2011
23. c 25 8 RI RI 9 10 11 12 d c RI RI 8 Tracer 2 9 10 12 13
24. 22 Tracer I 4 2 16 30 5
25. Tracer I 4 2 16 30 5 gt
26. X X 2 P 20 X X X X X X
27. 18 3 5 6 50 20 9 30 16 30 9 30 17 00 5 2 3 5 5 I 2
28. 3 PomA PomB PomA PomB cell free co PomA 2 PomA PomB DNA pomA pomB PCR 0 46 w v CHAPS 0 125 w v sucrose monocaprate 0 009 w v 7 DodecyHB D maltoside 0 58 w v n Octyl B D glucoside PURESYSTEM cell PomA B whole cell cell free Syn UF Ni NTA gt 100kDa cell free syn sup ppt cell cr PomA with liposome L o phosphatidylcholine 3 PomA PomB DNA pomA pomB PCR cell
29. T 12 Tracer P P P 500 2 bE f nayoshi Y Okino Y Miyake K Mizutani A Yamamoto Kishikawa Kinoshita Y Morimoto Y mamura K M Morshed Kono K toh T Nishijima K Ijima S Transcription Factor YY1 Interacts with Retroviral I
30. 4 X 94 13 00 13 20 13 30 16 30 1 1 30 i NM I 2 2 30 3 X X 2 1 X 2 2 2
31. 2010 11 25 26 1984 5 G7
32. PomA PomB MotX MotY 4 1 H or Na A Nl srinb eus db ll J SS 1 A B PomA B P T MotX MotY PomB PomA 4 PomA PomB 2 otX MotY
33. 1 2 0 3 16 30
34. 22 9 23 1 eg 24 27 23 2 22 10 12 3 9 8 15 TA TA TA TA RI 28 11 30 13 X 14 I 25 3 17 61 Tracer 3 0 21 2 199 51 0 0 24 12 21 2 223 63 ws 3 ym mr ba 22 9 10 22 9 29 22 10 29 22 11 15 22 12 7 lt 23 1
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37. MCV 131BNF Typhoon FLA 9000 GE EC MA 3 24 1 9 RI BAS1500 RI 3 473 5
38. 7 PomA B
39. RI 4 5
40. RI 40 27 25 26 lt
41. 5 c 25 11 d 7 a 3 RI RI 3 4 6 7 4 5 b RI RI 30 a 6
42. 22 9 22 22 10 19 22 1 12 22 12 9 23 1 18 23 2 18 RI X a Hi 22 9 15 Y umi Eu mul HH 22 10 21 22 10 21 1 SU BB 23 1 13 22 9 16 22 10 22 23 1 22 10 14 19 22 1 11 22 11 11 22 11 10 22 12 22 10 10 1 1 20 22 10 11 29 19 22 12 12 1 17
43. 22 2 Tracer S I gt
44. RI 22 9 16 2 22 10 2 23 1 14 22 10 19 22 11 11 X 2 11 10 22 12 1 67 19 76 Tracer 23 1 5 2 3 2 Ms aR 0 RI R 1 X HI X 2 RI RI en X P 22
45. IP 2 1 IL ATP P ATP 2 Vol 49 2011 77 RI RI YP PpP
46. PomA PomB Na 1 Terashima H Kojima S amp Homma M 6 Tracer 2 3 4 5 6 7 8 9 2008 Flagellar motility in bacteria struc ture and function of flagellar motor Int Rev Cell Mol Biol 270 39 85 Asai Y Kojima S Kato H Nishioka N Kawagishi I amp Homma M 1997 Putative channel components for the fast rotating sodium driven flagellar motor of a marine bacterium Bacteriol 179 5104 5110 Terashima H Fukuoka H Yakushi T Koji ma S amp Homma M 2006 The Vibrio mo tor proteins MotX and MotY are associated with the basal body of Na driven flagella and required for stator formation Mol Microbiol 62 1170 1180 Endo Y amp Saw
47. Tracer 49 23 3 30 464 8602 052 789 2563 FAX 052 789 2567 28 Tracer
48. 3 2012 3 9 8 9 3 9 2 10 3 10 3 12 4 12 2 26 Tracer 23 3 1 ey Cl 90 NIJIuUil TY GR x od R 143 23 1 27 1 2 1 23 Vol 49 2011 Tracer 49
49. PpP Cr Im 1 HC RI RI 50 84 200 11 25 11 18 1 107 12 3 a 1 b RI RI
50. 11 RI RI 14 64 2010 4 26 2010 7 8 17 12 RI 4 No 61 1 100 3H 14G ACSI pH 4 26E 07 1 95E 06 4 3H 1521Bq ml 14C 698Bq ml Ph4 15 Vol 49 2011 1
51. 5 30 70 Tracer
52. pomA pomB Syn FT W E1 E2 pomA pomB his Syn FT W E1 E2 ce PomA o PomB 5 PomA PomB Ni NTA CHAPS Ni NTA PomB Hiss 300mM syn FT W E1 E2 PomA PomB PomA PomB PomA PomB CHAPS PomA PomB 4 PomA PomB PomA PomB 5 PomB C PomA CHA
53. PURESYSTEM PURESYSTEM His tag DNA PCR PomA PomB PCR 2 Ni NTA PomA PomB PomA PomB 2 CMC CHAPS sucrose monocaprate 7 DodecyLB D maltoside z OctyLB D glucoside CHAPS PomA PomB
54. X X Xx i P 18 2 X P 22 T 3 X i ee RI pe HP Vol 49 2011 7 I RI I 1 1 2 80 4 9 00 9 20 9 00 9 20
55. 2 RI Vol 49 2011 19 I 5 RI
56. 19 23 2 14 22 10 4 22 10 6 22 9 6 22 9 7 22 12 20 22 12 24 RI 53 11 Vol 49 2011 Fl Ny Ee Ea lt a Ok Nm UH 1 RI 22 9 15 2 23 1 14
57. 25 mM 90 pl Na Dowex 50WX8 400 Sigma Y PomA B Na 630 ULO assay buffer 10 mM HEPES KOH pH 7 5 200 mM choline chloride 5 mM MgCl 10 w v glycerol and 0 5 mM NaCl Na 14 8 kBq ml 70 ml 30 C 10 20 mM 90 Na Dowex 50WX8 400 Sigma Na PomA PomB
58. PomA B whole cell cellfree syn ppt 50mM MgCb sup PomA PomB PomA PomB PomB PomB Tracer not solubilized solubilized by 1 CHAPS oc PomA Ba 1 co PomB 4 PomA PomB 1 CHAPS cell PomA B whole cell syn L P L S U P U S
59. 32 635 nm 685 nm 18x24cm X Tracer VICTREEN 451B Gamma Reference Sources 9 Gamma Reference Sources 9 Ba 133 Fe 55 X JRIA FE405 H X NHC410B1 1
60. Nucleus Participate in Generation of Periodic Oscillation of Neural Activity Driving Pulsatile Gonadotropin Releasing Hormone Secretion in the Goat The Journal of Neuroscience 30 8 3124 3132 2010 Okamur H Murata K Sakamoto K Wakabayashi Y Ohkura A Takeuchi Y Mori Y Male Effect Pheromone Tickles the Gonadotrophin Releasing Hormone Pulse Generator Journal of Neuroendocrinology 22 825 832 2010 Hoshino M Tanaka T Nakamura T Yoshida H Saito T Tsukada K Katsurada Y Aoki Y Oho S 14C Tntegrated Research in the Bishr Mountains on the Middle Euphrates Newsletter 18 15 16 2010 Radio Guide Way 676 68 71 2010 J HayashiH Kojima Y Shibata M Kawade K Practical Q analysis method based on the Fermi Kurie plot for spectra measured wi
61. PS NiNTA PomB His PomA PomA PomB A PomA only a PomA B PomB only c PomB C PomA PomB PomB oe PomA o PomB PomA 5 PomB PomA PomB incubated at 30 C for 20 hr o PomA a PomB lt PomA molecular size marker 669 440 158 75 43 29 kDa 6 PomA PomB A PomA B PomB C PomA PomB CHAPS superdex 200 10 300 GL GE healthcare D PomA PomB 30 20 CHAPS PomA PomB AB PomA PomB 6 PomA fract
62. RI GEM35ISO Ge Vol 49 2011 25 2011 4 1 4 4 2012 4 4 1 3 1 10 5 2 3 2012 6 3 27 7 7 29 8 2 8 17 9 2010 2011 10 10713 14 11 12 12 22 0 4 1 4 3 2012 1 4 1 4 6 2 6 3 7 2 7 1 2
63. Tracer 49 GN SE 1 PomA B Na 3 ee 7 ME Ll 22 13 EE 14 SE EE 23 ee 17 HEA A NE 24 os 25 tunettvnntnerttnutrteeieturreesssseeseeesevess 26 2 27 RE 27 ml 15 2003 60
64. asaki T 2006 CelLfree ex pression systems for eukaryotic protein DrO duction Curr Opin Biotechnol 17 373 380 Shimizu Y Kanamori T amp Ueda T 2005 Protein synthesis by pure translation sys tems Methods 36 299 304 Nomura S M Kondoh S Asayama W As ada A Nishikawa S amp Akiyoshi K 2008 Direct preparation of giant proteo liposomes by in vitro membrane protein synthesis Biotechnol 133 190 195 Yorimitsu T Sato K Asai Y Kawagishi amp Homma M 1999 Functional interaction between PomA and PomB the Na driven flagellar motor components of Vibrio algi nolyticus Bacteriol 181 5103 5106 Sato K amp Homma M 2000 Multimeric structure of PomA a component of the Na driven polar flagellar motor of vibrio algi nolyticus Biol Chem 275 20223 20228 Terashima H Abe Yoshizuml R Kojima S amp Homma M 2008 Cell free synthesis of the torque generating membrane proteins PomA and PomB of the Na driven flagellar motor in Vibrio alginolytcus Biochem 144 635 642 1 19
65. ion22 PomB 75 kDa PomA PomB 30C 20 PomB 158 440 kDa fraction18 20 PomA PomB PomA PomB Na PomA B Na assay buffer 20 mM Vol 49 2011 5 Tris HCl pH 8 0 200 mM Cholne CL 10 w v glycerol 1 mM DTT 1 mM NaCl Na 14 8 kBq ml PomA B 10 30C 5 10
66. neurohypophysial diabetes insipidus Am J Physiol Regul Integr Comp Physiol 298 2 R486 93 2010 Shimizu H Arima H Ozawa Y Watanabe M Banno R Sugimura Y Ozaki N NagasakiH Oiso Y Glucocorticoids increase NPY gene expression in the arcuate nucleus by inhibiting mTOR signaling in rat hypothalamic organotypic cultures Peptides 31 1 145 149 2010 Rahman M A Senga T Ito S Hyodo T Hasegawa H HamaguchiM S nitrosylation at cysteine 498 of c Src tyrosine kinase regulates nitric oxide mediated cell invasion J Biol Chem 285 3806 3814 2010 1 f Amano M Tsumura Y Taki K Harada H MoriK Nishioka T Kato K Suzuki T Nishioka Y Iwamatsu A Kaibuchi K A proteomic approach for comprehensively screening substrates of protein kinases such as Rho kinase PLoS One 5 1 e8704 2010 Itoh N Nakayama M Nishimura T Fujisue S Nishioka T Watanabe T Kaibuchi K Vol 49 2011 Identification of focal adhesion kinase FAK and phosphatidylinositol 3 kinase PI3 kinase as Par3 partners by proteomic analysis Cytoskeleton 67 5 297 308 2010 13 22 9 23 2
67. ntegrases and Facilitates Integration of Moloney Murine Leukemia Virus cDNA into the Host Chromosomes Jounal of Virology 84 16 8250 8261 2010 Liangl X Wakuda R Fujioka K Asanuma H Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism FEBS Journal 277 6 1551 1561 2010 Nakane Y IkegamiK Ono H Yamamoto N Yoshida S HirunagiK Ebihara S Kubo Y Yoshimura T A mammalian neural tissue opsin Opsin 5 is a deep brain photoreceptor in birds PNAS 107 34 15264 15268 2010 Tachibana T Moriyama S Takahashi A Tsukada A Oda A Takeuchi S Sakamoto T Isolation and Characterisation of Prolactin Releasing Peptide in Chicks and its Effect on Prolactin Release and Feeding Behaviour Journal of Neuroendocrinology 23 74 81 2011 pir Ll gt 1 Wakabayashi Y Nakada T Murata K Ohkura S Mogi K V M Navarro D K Clifton MoriY Tsukamura H Maeda K R A Steiner Okamura H Neurokinin B and Dynorphin A in Kisspeptin Neurons of the Arcuate
68. th total absorption BGO detector Nuclear Instruments and Methods in Physics Research A613 79 89 2010 rh Ban R MatsuzakiH Akashi T Sakashita G Taniguchi H Park S Y Tanaka H Furukawa K Urano T Isotope News 2010 2 57 58 2010 Mitotic regulation of the stability of microtuble plus end tracking protein EB3 by ubiquitin ligase SIAH 1 and Aurora mitotic kinases J biol Chem 2009 Sakaidani Y Furukawa K Okajima T O GlcNAc modification of the extracellular domain of Notch receptors Methods in Enzymology 480 355 373 2010 ArimaH Oiso Y Mechanisms underlying progressive polyuria in familiar neurohypophysial diabetes insipidus Neuroendocrinol 22 7 754 757 2010 Hiroi M Morishita Y Hayashi M Ozaki K Sugimura Y Nagasaki H Shiota A Oiso Y Arima H Activation of vasopressin neurons leads to phenotype progression in a mouse model for familial
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