CELLMAX ®® DUO 中空糸細胞培養システム
Contents
1. 37C 1 lt 22. ELISA 48
3. 1 mg VaxXG V XG V XGJ t V V
4. CelIMax Duo CellMax Duo ECU 2 mw LC M 25 4X33 02X16 51 cm 2 25 kg 100 240 V 50 60 Hz 0 8A 5 120 m0 min 20 42 C 100 CO Cat5 5m0 min CellMax Duo
5. 1 ECS ECS 2 3 Clave luer 4
6. CellMax ECS 4 v SG ECS
7. 1 luer
8. ECS T ECS Conditioned Medium 20 30 CellMax Duo 100 145
9. 2 ECS 1 5 3 0 7 0X107 luer g 1
10. 3 4 5 125m0 160m6 35m0 6 125m6 500m0 1000m0 7 10 500 1000m0 day 7 10 7 5 5 0 2 5
11. Duo luer 12 Iuer CelIMax Iuer 12
12. CellMax amp N Clave
13. p 25 2 0 g 9 RPML 1640 1 0 1 5g 9 1 0 0 50g 6 4 5 g 9 DMEM 2 0 1 5g 0 3 0g 9 2 5 17 MWCO ECS ECS
14. 2 4 100m9 ECS Clave
15. 121
16. 1 Duo 2 3 4 5 37 48 ECS 6 15 30 ECS
17. 2 mg day 4 5g 6 PDMEM pH HEPES pH 2 0g 0
18. Colon LS174T CEA Colon WIDR CEA 23 Si 2 Bs 3 ED ET Si FR 3 uy Breast Mammary Mammary Embryo Breast Fetal Skin Breast Tumor Melonoma Ovarian Ascites T T pH 0 2 HB 4 F E
19. 9 luer luer luer luer
20. 90 1 ECS Conditioned Medium ECS 20 30 ECS 1 Clave
21. 125m0 33mm 38mm W 1 7 125m9 38mm 38mm luer
22. ECS ECS E OE 1 5 1 10 10 CellMax CO2
23. Microfnge 5 50m0 ECS ECS 20 30m0 ECS 1 50m0 25 50m0 2 20 30m0 19
24. ECS ECS 1 12 luer 13 14 CellIMax 30 60 ECS y ECS 10 25m0 50 100m0 1 y
25. 6 26 V g 9 G G g 9 G g 0 te t CellIMax 430 011 RPMI 1640 FBS IgM ECS 5 0X10 Tracer FBS mg 24h m
26. 1 0 3 0 80 ZXOT 60 E 50 40 G 30 20 9 0 ll T T T T T T 5 65 YA 8 9 10 11 12 13 14 16 17 18 19 CellMax 1 0 5 1 0X10 2 0 5 0X10 T 150 50 100 CellMax
27. 10 30 50 m6 Luer Lok ECS luer 15 25 4X38 1cm luer Luer Lok luer 125 250m0 L CellMax Duo Duo Duo 100
28. Duo ECU ECU ECU ECU LED Duo 2 Duo CO ECU BCU ECU ECU Duo
29. CellMax pH CellMax E CO 22 37 CellMax ECS 1 5 0X10 lgG IgM 20 60mg day ECS 1 0 5 0mg m0
30. CellMax Duo CO pH pH CellMax Duo ECU CO 5 0 10 a 15 1 0 T 37 ECU Duo ECU ECU
31. ECS 1 5 ECS 100 300mg day 3 4 CC 1500mg day NZ 2 0 g 9 RPMI 1640 1 0 1 5g 9
32. lt lt v 2 33mm 1
33. CellMax Duo 0 120m0 min 1 37 5 10 2 24 1 0 X10 5m0 min ECU 1 1 0 5 0X10 25m0 min ECU 4N 5 0 10 0X10 50m9 min ECU 8 3 24 50mg day 5m9 min 50 200mg day 25m0 min 200
34. E ee ox ECU Fu Duo 1 50m9 min ECU 5 NII NR 2 3 0 x 10 24 25m6 min Te 16 1 2
35. Bone Marrow Osteoblast MCF7 SF9 primary A431 NMuMg C127 NIH 3T3 N Ras primary primary IE primary primary Heart COL 12 COX END 2 NOT TAC 5 AA3A OKT 1 Daudl E HL60 TIL TL TIL U937 L8 J558 Transfectoma Transfectoma CHO T 24 Cells Cells Growth Factor Ea Cells Cells EX Proprietary TGF w le Pro Insulin Growth Factor 3E Growth Factor Growth Factor 3 Cells Hyulornic Acid EA Cells
36. 4 5 ECS ECS 6 CELLMAX Oerecmo 6 1 4 ECS 14 10 11 12 13 ECS
37. ECS luer luer 11 Own 4 ECS 8 4 5 ECS ECS ECS 5 5 ECS 9 7 10 11 luer
38. Tt i 3
39. i ECS ECS 1 CELLMAX on en mw seecrnow 2 10 2 4 Clave ps CELLMAX 3 50m0 25m0 HH 20m0
40. ECU LED ECU 1 LED 2 1 AC j jJ i 2 Cat5 ECU Duo W
41. 490m0 8 500m0 mg m m9 m6 0 mg day 0 4 00 PM 120 0 0 6 10 00 AM 120 1 08 120 22 5 7 10 00 AM 240 0 94 250 96 0 8 2 00 PM 490 0 88 500 175 7 1 mg day V XL V XL V XL t t 6 120X1 08 0 x0 120 x0 5 75 0 22 5 mg day 7 240X0 94 120X0 120X1 08 6 75 5 75 96 0 mg day mg m0 g 0 V XL FBS 10 0 160mg 0 FBS 5 0 80mg 9 2 X V XL 1 V XL 1 Pan D R Shankar D F Stroncek and C Whitley Hum G
42. ECS 1 5 Clave 4 BCS ECS Clave luer luer
43. luer CellMax AC 5 lt 90
44. ECS ECS ECS ECS ac 18 20 16 17 18 19 19 21 22 22 23 24 25 25 26 26 26 26 28 29 H w CellMax Duo AC100 240 50 60 Hz CellMax Duo ECU Duo
45. y CellMax Duo ECU Duo Y CellMax Duo ECU 8 FE ECU ECU BCU 1 y CellMax Duo 1 3
46. 50 100m0 1 BCS Y 30cc 60cc 20cc 20 luer
47. NM CellMax Duo 2 2
48. 3 4 5 luer 6 7 ECS 8 9 10 50m 11
49. 1 5 1 10 7 10 1 500 1000m0 2 1 pH CellMax MI
50. 1 0 0 5 g 6 y 4 5 g 9 DMEM 2 0 1 5g 0 2 5 3 0g 0 ECS ECS CelIMax ECS 1700cm ECS IgG ECS IgG
51. lt CellMax luer luer 2 luer luer luer 4 5 125m0 100m0 35m9
52. 5 0 8 0X107 pH T 2 7
53. ECS ECS ECS luer 0 2 0 5um 21 3 2
54. ECS 25 24 48 ie pH
55. 1 0 2 0 1
56. 2 15 20m9 5 20 75 80 ECS 750 2000mg day luer
57. Il L mg m0 ng m0 0 4 120m6 85m9 35m0 28 6 1 08mg m0 120m6 V 240m0 7 0 94mg m0 250m0
58. gG a gG IgM gM gG A a gG gG Cells Cells KNU EEI Cells Growth Factor 2 Cells Protein 2 Cells IgA Protein Protein 3gaK Protein 3 7TT 1 ECS ECS 10 15 1 0 3 0 CellMax
59. 2000mg day 50m0 min 2 15 1 CellMax Duo 0 120m6 min ECU i ECU CELLMAX DUO VARIABLE FLOW SETTINGS
60. 60 CellMax 1 CellMax 1 2 10 20 30cc 2 2
61. IgG 1 10 m0 1 45 mg m0 14 5 mg 56 2 12 m0 0 65 mg m0 7 8 mg 30 3 12 m0 0 3 mg m0 3 6 mg 14 ECS ECS 1 ECS 10 20m0 18 1700cm 1 ECS 11 15m0 60 75
62. 0 1 55 125 100 12 2 120 125 125 12 3 150 500 500 12 5 230 500 NONE 12 1 2E 08 6 330 500 500 12 7 246 500 NONE 10 8 486 500 500 10 9 0E 07 9 540 500 500 10 10 650 1000 1000 10 1 4E 08 13 560 1000 1000 8 1 0E 08 14 521 1000 NONE 8 15 558 1000 1000 6 1 4E 08 16 790 1000 NONE 6 17 854 1000 1000 4 1 5E 08 20 542 1000 1000 4 2 1E 08 21 976 1000 1000 4 22 1123 1000 1000 2 5 1 5B 08 23 1075 1000 1000 2 5 9 0E 07 10225 650 md 27 lz000000 1f000000000 FEHBS FBS UH0000000000mo0 1H 1 234S6Z8921011121314 1S 16 17 18 19 2 21 2223 0 VIXL V XL V XL As V 0 0 0 Vi L mg m0 0 03 g 9
63. 1 2 luer luer 30cc ECS 37 48 ECS 1 E 2
64. CELLMAX DUO SPE ES g001 2000 ay in BIC CERTIFIED Copyright 2009 Spectrum Laboratories Inc All Rights Reserved CellMax Duo ECU BCS ECS 2 10 10 10 12 13 13 13 13 14 14 15 15 16
65. Clave luer Clave Iner luer ller Clave 30 60
66. Clave 1 ECS 1 ECS 13 50m0 ECS
67. ene Ther 10 2799 2810 1999 2 Beck T L Schich S Sitlani and M Menaker Focus 17 2 5 1994 3 Bradbury MW Exper Physiol 1993 78 453 472 4 Cancila PA Bready J Berliner J Brain endotherial astrocytes interactions The Blood Brain Barrier Cellular and Molecular Biology Raven Press pp 25 47 Spectrum Laboratories Spectrum Japan 520 0105 3 12 18 Fax 077578 0166 e mail spectrum j gol com web http jp spectrapor com 29
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