pNCMO2 DNA - Clontech Laboratories, Inc.
Contents
1. Lot No RE ae C PES Brevibacillus FRALEY INI AOD BRR AR MOGAAKICOUW Cd Brevibacillus Expression System Il 54 II FHB200 DMIR E A Chea lt EU BHeCH lt EU 1 DVENFIVDARRY AT LAER als CTS RASA FE REL ADFREE THES 2 ARISEN COMERS EN TOES 3 ABC RES SMES TS SL rs L TOES ARmlsHABe UCRALTHVESF Ch BM ORR Hark Ramah pNCMO2 DNA 4 Brevibacillus EXMEODY v KINZ ATHY K BRIA CHIRT DAS Re Brevibacillus CHA KREACE GTEMURS pNCMO2 QRR TO E 2 cle TaE HO MEE VY 7 BRO P2 FOE 2 AWTWHS P2 FAE 2B lk Brevibacillus CHT dFE RICHY FTOE 2 ELTHSK DOD AAA TIDENE D EAn e LC LELYC lt HEEL BMBRFO 7O LY TCHR CHS FIR 10mM Tris HCI pH8 0 1mM EDTA R 5 225bp pNCMO2DNAD7O Y7IH7 FA AAGGCGCCGCAACTTTTGATTCGCTCAGGCGTTTAATAGGATGTAATTGTGAGCGGATAA P2 35 P2 10 lac operator CAATTATTCTGCATGGCTTTCCTGCGAAAGGAGGTGACACGCGCTTGCAGGATTCGGGCT SD1 TTAAAAAGAAAGATAGATTAACAACAAATATTCCCCAAGAACAATTTGTTTATACTAGAG SD2 Hpa GAGGAGAACACAAGGTCATGAAAAAAAGAAGGGTCGTTAACAGTGTATTGCTTCTGCTA C M K K R R V V NS V L L L LL m Neo Pst BamHI Sall TGCTAGCTAGTGCACTCGCACTTACTGTTGCT CCCATGGCTITCGCTGCAGGATCCGTCG L A S A L A LT V A P M A F A A Signal cleavage Cloning site Xba Xho EcoRI Kpn Sma BstB Cla Hind III ACTCTAGACTCGAGGAATTCGGTACCCCGGGTTCGAAATCGATAAGCTTCGGCATTATAG 120 180 240 300 360 422. 0 WTI CISEA LEU KS CER TE Ke Bam bim R ANFI ADERE CROS gt BE BAR REOOO WE BARMOME RAT AT CMAIEEN THES FLEAS SRMO RISD TT DS OVS CRS ECW BU heken COSASHAKEOMMAE ClE BHO KML BER AHS L lt SRERROMR CHU TNE Slcm RB LES P2 FOE 2 lk ha BAV IN 7SGKhOF BESOE 2 CH 2 TOFOE 2 OB SEHE lac HAN L 2 IERA LY ARRACOFOE A TEV tlt LTO b SD Belk 2 PAT L SD1 SD2 fl CIL LCHREN Bo The P2 promoter isa major promoter for transcription of cell wall protein genes In E coli cells the activity of the P2 promoter is controlled with ac operator present in the upstream region of the promoter The two SD sequences SD1 and SD2 in the P2 promoter are transcribed at iden tical frame v201307 Tel BER EO anus TaKaRa r7 AIVYR hkoty 077 543 6116 Fax 077 543 1977
3. Brevibacillus Expression vector pNCMO2 DNA Code No HB112 Size 10 ug 0 2 OD Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Concentration 0 2 ug l Volume 50 ul Description pNCMO2 is a shuttle vector for Brevibacillus and E coli Accordingly the expression plasmid is constructed in coli after which it is introduced to Brevibacillus for protein expression The P2 promoter derived from a cell wall protein of the host bacterium is used as the expression promoter for pNCMO2 Because the P2 promot er does not work in coli it is useful in the cloning of the target gene However it is an exceptionally strong promoter in Brevibacillus resulting in efficient protein production 10 mM 1mM Form Tris HCl pH8 0 EDTA Base pairs 5 225 bp Usage This is a vector for protein secretory expression using Brevibacillus Ex pression System a shuttle vector between B choshinensis and E coli Protocol Regarding protocol please refer to the product manual supplied with Brevibacillus Expression System II Cat HB200 Cloning sites of pNCMO2 DNA Please refer other side of this sheet Map of pNCMO2 DNA P2 promoter sec signal peptide multiple cloning site Ro eee X terminator ori ColE1 ori rep Amp Nm NOTICE TO PURCHASER LIMITED LICENSE L39 Brevibacillus Expression System 1 Brevibacillus
4. Expression System related products were developed and manufactured by Higeta Shoyu Co Ltd and sold by TAKARA BIO INC 2 The use of this product is allowed for research use only 3 All rights of intellectual property related to this product are owned by Higeta Shoyu Co Ltd Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements All trademarks are the property of their respective owners Certain trademarks may not be registered in all jurisdictions v201307 FLENFIVAZARRANT a pNCMO2 DNA Code No HB112 ORR Size 10 ug 0 2 OD Shipping at 20 C OR AA Store at 20 C DICE L BENE 2 EAA EET OPNCMO2DNADNDZ Ny 7 OST LYAlLIWUT 8 EOECREW L39 Brevibacillus Expression System BNMMECH lt ECL BNMMHZCH lt EE
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