ミニプロティアン 3セル - Bio-Rad
Contents
1. 115 11 11111141111111411 5 000000000 000000000000 190000000000 a Note E 5 phot asa gi 1000 i 000000000000 A fe PE 1100 1000 10 100000 Rio 1000033 0000000000000000000000000000000000 0000 111 ae 0 1000 10 380 0 Note 00000000000000000000000000000000000 000000000000000000000002. JUUU Native 000000000 1
3. 0008000000000000000000000000000000000000000000000000000 1 101011010 ef 4
4. 0 0 0000 1 0000 000 000 pog0000000000 JUUU IODO 00000
5. 721 1 1111111111 111111 11111111 11101 0 0 Native PAGE NativePAGE SDSPAGEG OOOO000000 NativePAGE 1 1 111111111 1 1 1 1 1 1 1 pH DO pHI pl NativePAGE 00000 PHO
6. 0000000000 1 5M 1 0000000 375M 1 UU
7. 0000000000000 6 _ 0000000000000000000000000000000000000 OO d000 000000 0000000000 0000000000000 0000000000000 10 0000000 00 0 00000 00 00 0 0 180mM 000000000000 000
8. 1111 111 11111 11 11
9. 10 30 0 267 87 69 29 2g 100 249 0 8g 100mI DD 300mI 00000 1 1 11 1 1 pDo00000000000 3750 10 1509 UU 161 0125 3 00 3759 19 500 161 0158 30 0 O 37 50 1 500m1x 2 O 161 0159 2 5 8 1 0 3 0ml 05 w v 0 2 Total Volume 10 0ml 00000000000 UJU
10. 1800000 1000000000 J Note 000O000000000000000000000000000000000000000000000000000 9 oooooooooooooloooloooloooooooooag uu
11. NotegOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOA OOoOOOOOOOO O00 223 9910 NoteQOQOOOO0000000000000000000000 Notei
12. 0 5M Tris HCl pH6 8 JUD 69 O 60ml eng ACI pies 00000000000 10mp 00000 G 00000000 0 Tris 690 1 11 11 11 11 11 1111111111 11 05M Tris HCID pH68 OO 10000000 161 0799 SDSU 0000050 50000000 3 55 05M Tris HCl 8 125ml 2 5 w v SDS 2 0 10 O50 0 2 Total Volume 9 5ml 1000000000 H Laemmli 000000000 3000 00000 1610737 950 50 311 1 10 00000000 20 000 0 0 95 0000 1 111 1 1x 25mM 192mM 0 19 5 pH8 3
13. 1 1 CO 1 0 0 SDS PAGE
14. mod000000 00000 CI 5 OU IOD 0000000000 DD 1 5 160 661 661 400 7300 30 680 1 0 105 44 44 26 4204 0 75 700 33 33 200 0
15. 1400000000008 00000 000000000000 00000000000 00000000000000000000 0000000 0008000000000000000000000000 xf 1 30400000000 DO0O0O00O00000C000000000000000000000000C00000000000 TFB NRE PR iD 1000000000 TEMEDJAPSJ0 0 0000 0000 0001500000000 1 100000 poo 0000000000000
16. a 00000000000 000000 00000000000 o0d0d0 00000000 000000000 0000 0 0000000000 0 O0 00 00 0O00 0 075 1 0 0000 000000000 00 000000000 0000000000 00000000000 0 OU 00000000000000000000000000 00 000000000 000000 YU 00000000000
17. 1 1 1100 0000000 000 NoteQOOOO00000000000000000000000000000000000
18. 1 1 x 100 1 11 11 1 26 DU OOOOO 1000 759 002000000000 4 15 10 20 00000000000 11111111 1 1 Dgo 1000 0 55 061
19. 0000000000000 SR 0000000000000000 0000000000000000 0000000000000000 0000000 480 000000000900000000 0000000000000000 0000000000000000 D000000000000000 00 000000000 Note JD0O000000000000000000000000000000000 00000000000000000000000000000000 000000000000000000000000000000 000000000000000000000000 40 a NoteJ J0D0000000000000000000000000000000000 ooo O O gt 00000000000000000000000000000000000 0000000000000000000000000000 00000000000000000000000000000000 0000000000000000000000000000000 000000000000000000000000000000 000000000000000000000000000 440 NoteJ J00000000000000000000000000000000000 Do O O d
20. 000000000000 00000000 00000 0000000000000 0 00000000 SG 111111 0 0 0000 Dodd 000000000 00000000000 Dodd 0 0000000 00000000000
21. BERRA 04010 101410 0 0 00100000000000 mm mi 0 56 280 075 42 1 56 15 84 6 0 0 SDS PAGE Laemmi5 U UU 0 11301111 1111 11111111 1101 301 11 26711 000000 87690 2929 100mI 246 086 100mI 300mI JUD 00000 1000000000 1000 3750 19 15 0 161 0125 300 0 0 3750 19 50010 0 0 0 0 0 D 161 0158 000000 300 0 3750 19 500mlx 270000000 161 0159 20 100 w v
22. 1800000000 pHO O 1 O0 pH 11 11111 11111111 1111 111 11 1 1 pHo pl 1 1 1 11 1111 11111 1 1 Native PAGEJ 00000000000 1020000000000 Ornstein Davis 3 000000 0000000 000000 1 1 Note Ornstein Davis
23. 6 6 a nos Dx 00000000000 116 0014 222 0033 305 0031 532 0025 465 0093 812 0013 BIO
24. Catalog Numbers 165 3301 165 3302 00000 000000000000000000 1 1 1 1 I TO 1 SO SE SN PI IT 0 1 51 2 TI 3 P DD O Ec 7 3 1 11 0 4303 4 E 4 BKC BES kB SE SR RD GD OEA A O ED OSCE ees 29 11 509 9 6 UG 11 1 111151131 11111111110 7 SE ee 8 3 11 8 O SDS PAGE 00000 10 UD NGUVEPAGE 25 77 11 10 PDH D 7 9 7 7 7 7 11 177 11 0 SDS PAGE Laemmli DO DDD 994299 9999e99eeee enna 1 000000000 NativePAGE
25. Od 40000000 1000 10x 161 075 10 10x DU O 161 0772 3 3 50 4 70100 1009 1 10m0 O 0 0
26. EN61010 10 0000000 OOO 1111111111 1 11 111111 0000 11 EN61010 10 000000000 EN61010 1 1
27. 40000000 50000000000000000000 ja 20000000000 1 1 0 4 00000 6 010 00000000 El a E oo oo DO oo cta e E EF oO o 0 290000000000000 0000000 DU bJUDDDDDDUDUDUD 000000 DU all lo FEI 1121 0000000000 b 0 b 1900000000000 000000000 bOOOOOPMSFOOO0000000 b 0000000000 a 8 11
28. 5 00000000 Chambach and J ovin OOOOOOOOOOOOO NoteQQOO000000000000000000PN0000000000000 20 000000 11 1 PHU 11 11 1 11 1118118181 1 11 101 1 11 11 11 1 0 O O 100000 McLellan pH3 8 10 2 DU 0000000044
29. 0 O 161 0757 10 JO 00000 161 0771 poopo0p000000010x000000050mp9000 450mp00000000000000000 000000 1 0 1 0 0 0 0 0 0 0 0 0 10 50000000000000000000000000000 5 100 10 1000 309000000000 m0 00000006 mp 626 130 250 5 589 1 25 6 550 200 259 78 52 23 25 81 48 27 25 a 45 30 250 107 42 33 25 OO000000001 5M 7 6 pH8 8 00000000000 5M 5 1 pH6 8 Es 3 UU 00000000 Native PAGE
30. 170 3935 000 000000000 165 3301A 2 165 2965 100000000 165 2976 00042200000000000 165 2977 4220 000000000000000 60 0 5 0 75 1 0mm 15mm Swell OO 165 3327 165 3332 165 3338 9 165 3328 165 3333 165 3339 10wellg OO 165 3325 165 3329 165 3334 165 3340 15 0 165 3326 165 3330 165 3335 165 3341 prep 2 DO 00 165 3331 165 3336 165 3342 IPGOOO 165 3337 165 3343
31. 5x O 85 7 45ml L 174M 115ml L 174M 5 75ml L 0 MES 195 2 29 39 L MOPS 209 3 314g L HEPES 238 3 41 7g L EPPS 252 3 37 85g L O 0 6183 7 739 L CA PS 221 3 44 26g L CA PS 221 3 22 139 L 800 20MM 40mM 20mM 30mM MES 30mM MOPS 35mM HEPES 30mM EPPS 25mM O O 40mM CAPS 20mM CAPS mo 2800000 20 20 20 ellan McLellan 38 pH102 pg0000000000000 lun 5x B 89 09 1336g L 8 0 000 89 09 35 649 L GA BA 103 1 4124g L 155 2 23 289 L 15
32. 1 090000 00 0 0 00 00 000 00 0 00 H 000000
33. 1 38 5 54 4 8 13 5 50 111111 1111580 17 a a ts sas 17 ee 560 18 SH RU 0 11 20 000000 o 0000 00000 m000 000000 000000000000 0000000 00000000 00 0 0000000 010000000000 00000 00000000000 0000000000 00000 000000000000 0000 00000000000
34. Note P0000000000000000000000000000000 IUUUUUUUUUUUUUUU IUUUUUU 03 1 1 1 1 1 ga 20Y7900 0080000000000 00000000000 00000 00000000000 000000 NativePA GE SDS PAGE uu n 03 0000
35. Note00000004 T000000000000000000000000000000000000000300000 0000000000 000 mo 30000000000 mo mig 10 5050 ml 4 610 130 250 0160 5 510 174 250 010 6 54 20 25 0160 7 510 230 250 0160 47 27 25 0160 A 440 300 250 0160 10 410 330 250 0160 111 370 370 250 0160 121 340 4 06 250 0160 13 310 4 360 250 0160 147 270 476 250 0160 157 240 500 250 0160 167 210 530 250 0160 170 17 57 25 01 0 000000 1 5M Tris HCl pH8 8 0000 00 0 5M Tris HCl pH6 8 0 0 000000000 Native PAGE Ornstein Davis 10 300 000000000000000 30000267000 2920 100mI 086 100mI 0 00 0000000003 0 0 0 00 0000000000 00 000 3750 0 161 0125 1030000 375
36. 165 3303 O ooo DO 1 165 3304 000 0000000 1 165 3305 1000 2 165 3308 1000 5 165 3309 000000000 5 5 165 3310 O 075mmpp00000000000 5 165 3311 1 0mm 5 165 3312 1 5mm 5 165 3306 1 165 3307 O 0000 1 165 3201 10 0 9 0 1 165 3146 10wellgg 1 165 3132 15 O 1 165 3130 O 0000 2 165 3149 2 165 2975 165 2949 O 0 5mm 0 75mm 1 0mm 15mm Swell O O 165 3352 165 3357 165 3363 9 165 3353 165 3358 165 3364 10wellg 165 3350 165 3354 165 3359 165 3365 15wellg 0 0 165 3351 165 3355 165 3360 165 3366 prep 2 DO 00 165 3356 165 3361 165 3367 6 0 165 3362 165 3368 1 000000000001 2 0000000000000 0000000000000 3 0000000000000 000 4 0 000000 5
37. 8 SDS PAGEQ OOOO0000000 1 1 1 10000000300 165 3301 00000000300 DO O 01 JUD DO 1 100000 120 075 EN 10 075 2 165 3302 18000000 oo O poo 1 1 1 165 3375 000 1 165 5050S PP300000 165 5050T 165 3317 00
38. 0 19 500ml 0 0 O 161 0158 DO 30 3750 19 500mx 20 161 0159 2000000000000000 01 5MTris HCIO pH8 87 IDO 27239 18 159 100
39. 00 1100000000 1000 gt 10 10 300 Note 0000000000000000000000000000 11 1111 11014111111 111111 11 00 01 OO JO 1 0 1000000000 0 00 TEMED APS 1500000000 iip 1 00 0 TEMEDO 1000 OD000000000000000000000000000000 Note 00000000000000000000000000000000000 0000000000000000 voo00000000000000000000000000000
40. 40pPH600000000TEMEDOOD50 10000000 00000000 11 1 Laemmli U K Nature 227 680 1970 2 Ornstein L and Davis B J Anal NY Acad Sci 121 321 1964 3 Chrambach A and Jovin T M Electrophoresis 4 190 204 1984 4 McLellan T Analytical Biochemistry 126 94 99 1982 111 10N KOHO O0 30000000 1 1111 11111 BEE 3 3 3 Poo 2 oOo oo ooo CO 2 00 Streaking 0 0 0 Er EIG a Oooo Oooo Ooog PT mo lt gt oof 53 ooo 53 nn was 3000000000000 000000000 000 000
41. 5 22 13 UU 1 1 1 1 O000000000000000 OOO 111 1 1 1 1 11 1 1 1 1 5 12 70 9 5 08 10 5 08 15 3 35 IPG 76 20 Prep 2D Reference well 3 10mm Sample well 71 70mm x 12cm Wi x 18cm HO IDO UUU 171 1 600V DC 17 00 50 C l pag00000000000000000000000000000000000000000 is an internationally accepted electrical safety standard for laboratory instruments 16cm L 11 600V DC 2 0kg uD n P0000000000000000000
42. 5 2 194g L O O 0 68 08 14 64g L 0 O 12114 19 389 L O 21 14 30 299 L 0 O 21 14 36 34g L 10000 48m 125ml L x 800000000 30mMB 80mMB 0 0 0 0 80mM GABA 30mM 25mM 43mM O 32mM 50mM 60mM 3 mM og 1067 0 TEMEDO 4 100000000 000m 309 000000 00 6 65 133 6 306 16760 6 05 2 00 5 JUL 380 440 430 614 669 7460 816 876 940 10 2 O pH 38 44 430 614 666 7460 816 876 940 10 2 Ams 10 APS 500 TEMED 100 Note pH6 TEMEDD pH
43. SDS 10g SDS 100 3000000000000000 01 5M Tris HCIY pH8 80 JUD 27239 18 159 100m 100 80 272390 Tris 80m0 0 0 0 0 0 0 0 6N0 HCO 888 00 0000 0 150m0 100000 10000000000000000000 15M TrisHCIO 88 0000000000 10000000 16107998
44. ml JO 80 27 2300 Tris 0 prH88 00000000000 150m0 0 000 0 c JDUUUUU 15M Tris HCI 88 0000000000 10000000 161 0798 300000000000 00 0 0 5M Tris HCIO pH6 8 JUD 6g OU 60ml o0 Tris 00000000 6Ng 686 00000 cag 11 1 11 111 1 Tris HCI pH6 sg 0000000000 10000000 1610799 40000000000 JO 555ml 05M ris HCI pH6 8 125ml 11 11 1 1 3 0ml O50 02 Total Volume 10 0ml 4 Native 100 39 161 0738 0D0O000000000 0000000000000000000020000000000 5010x 11 1 1 1 5 192mM 00000 pH8 30 IDO 303g L JU 1 144 09 729 L0 1000 1 10 10 IO 1
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