取扱説明書
Contents
1. D 105 0003 2 1 1 TEL 03 5521 5490 FAX 03 5521 5498 E mail biochemifa mail kikkoman coJp URL http biochemifa kikkoman cojp 2012 Kikkoman Corp 4 1 692. 1 43 3 RS 232C AC LED RS 232C AC AC LED IAC
3. ET
4. e 10cm e RS 232C OFF ATP ATP e 5 40 C 20 85
5. 0001 1000 1000 0001 OFF ON 0001 1 lt ENTER 47 5 1 2
6. Sey F Spa YES ENTER Me ENTER a F SA ENTER F 50 2 ENTER F ENTER
7. AC 2 RLU M pg CELL 2 1 x 10 2M 0 05pg assay ATP 1 1 x 10 2 3 x 10 7M ATP G 1 1 10 1000 ATP ATP M pg ATP
8. OSO RLU OOO OOO RLU D OFF POWER OFF 48 5 2 RS 232C BEEP RLU M pg CELL ATP CELL A E ATP CELL A E pg ON Gate Time
9. F C 110 51 NUMBER OOOO OOOO YES ENTER NO FUNCTION FUNCTION H ENTER
10. F ENTER y ax b a 1 00E 12 b 0 00E 00M YES ENTER NO FUNCTION 1 00E 17 1 OOE 10 b 1 0OOE O08M 0 00E 00M 60 ENTER ENTER 0005sec F ENTER YES ENTER NO FUNCTION
11. AN 8 1 OFF 8 2 3 OFF
12. AC gt mi S ae 1g OF eT AC
13. ENTER ENTER 0000 O ON GATE TIME
14. Iig fe A eal AN A 7J lt ly al ak gt OO k ke 37 Iig OF
15. TT A 10 OO ete RLU ND Not Detected 999 999RLU ATP 100 000 pg OFF POWER OFF
16. 100 6 3 CELL ATP M CELL 1ml ATP M 40 ATP mol CELL 64 7 C 1 10 C 1 10 RS 232C RS 232G 8bt o
17. 36 LUMITESTER C 110 OPERATION MANUAL Thank you for purchasing the LUMITESTER C 110 To use your LUMITESTER C 110 safely and correctly read this operation manual carefully and follows the instructions Kikkoman Biochemifa Company Ver 5 2 To ensure safe use Safet recautions WARNING WARNING indicates a potentially hazardous situation which if not avoided could result in death or serious injury CAUTION indicates a potentially hazardous situation which if not CAUTION _ avoided could result in minor or moderate injury or damage to the equipment In addition to the above alert symbols and signal words the following warning symbols are used in this manual to designate the degree of hazard and damage that might occur if this product is used incorrectly Mandatory This symbol indicates a mandatory action Prohibited This symbol indicates a prohibited action Electrical shock hazard This symbol indicates a risk of electrical shock Fire hazard This symbol indicates a risk of smoke emission or fire Explosive hazard This symbol indicates a risk of explosion Toxic hazard This symbol indicates a toxic substance risk gt gt ee POS Corrosion hazard This symbol indicates a risk of corrosion Handling precautions lf an abnormal condition is foun
18. gt AC AC gt AC 45 5 5 1 C 110 2 RLU M pg CELL 10 ON OFF RLU 5 1 1 ON pts
19. 1 2 66 9 10 1 1000 7 FR AC 700g 10 50 C 5 40 C 67 10 11 1
20. e s s e e 39 Nas AE HO
21. gt AC100V 240V 50 60Hz AC AC AC RS 232C AC100V 38 e e e
22. 1 1000 1 100 O RLU Count Rate PHETT 61 GATE RLU ON 0001 TIME 1000 4 ON ENTER GATE TIME F
23. e e es AC OF ae J Ax A
24. F 52 RS 232C ENTER RS 232C RS 232C RS 232C Wa ENTER Ses F RS 232C 7 FUNCTION 53 BEEP BEEP ON OFF ON OFF ENTER OFF YES LANGUAGE As ENTER BEEP LANGUAGE DATE a
25. ENTER ENTER GATE TIME 1 Osec GATE TIME 0 5 10 1 10 1 YES ENTER ON ON GATE TIME Count Rate 62 6 6 1 ATP RuU eR arpa y ax b x RLU y ATP M a b ATP a b a 2x 107 b 0 ATP a ATP b ATP ATP M RLU 63 6 2 ATP pg ATP M ao ATP pg ATP pg ATP E M x pg 5 07 x 10 507 x 10 x 1012 507 ATP 10 100
26. EE __ CSV C 110 RS 232C RS 232C USB USB RS 232C USB USB SRC06USM SRC06USB RS232C 1 5m OS USB RS232C peti Ae PC 32bit 64bit PC 65 8
27. F ENTER A 1 00E 18mol YES ENTER NO FUNCTION 1 OOE 20mol 1 OOE 12mol 58 pg pg M pg ENTER pg F ENTER 5 O7E 10 YES ENTER NO FUNCTION AAA 1 OOE 05 1 OOE 20 59 ENTER
28. IRS 232C cp O 44 lt POWER gt lt F gt ki kkomar LUMITESTER C 110 lt gt lt ENTER gt POWER 1 F ENTER 4 gt AC AC gt AC gt AC gt AC gt 4 AC gt DC IN
29. POWER LUMITESTER 2 NB D 1 5 5 AC ENTER er gee TEPEE 0000 OOO OOO RLU ig TL 10 ENTER 46 0
30. OFF F ENTER F F ENTER 49 RS 232C ENTER
31. These settings will not be reflected in STANDARD mode The start delay time is the time until the measurement actually starts after the lt ENTER gt key is pressed Procedure e Press the lt ENTER gt key and the cursor moves to the edit position e Change the values using the scroll keys e After making the changes press the lt F gt key e When a confirmation message appears press the lt ENTER gt key to enable the changes Input range Measurement time 1 to 1000 seconds Start delay 1 to 100 seconds 0 seconds cannot be set MEASURING TIME 0005sec START DELAY YES ENTER NO FUNCTION MAIN MENU If the value you have entered is outside the input range the cursor moves back to that position so re enter the correct value About measurement data display Values displayed in RLU relative light unit at the end of measurement are the average of the count rate measured during the measurement time 27 11 Monitor mode MONITOR MODE This mode displays the count value at specified time intervals GATE TIME during measurement The count value is displayed only in RLU units The count value is transferred to the PC if connected to the LUMITESTER When measurement is started with MONITOR mode set to ON the data previously stored in memory will be erased and the measurement data written in memory from No 0001 Measurement ends when the Measurement Time ha
32. ADVANCED mode ADVANCED mode sets the measurement time and displays count values in real time Note 1 Sensitivity and measurement range might differ depending on the reagent being used 3 Part Names and Functions 2 Cover 3 Measurement chamber 4 Display panel 5 Operation panel 6 RS 232C connector 1 Main body 7 Connector for AC adapter 8 Battery charge LED 1 Main bod The LUMITESTER main body containing the detector and y drive circuits 2 Cover The cover on the measurement chamber It can be opened and closed with the touch of a finger 3 Measurement chamber A sample tube is installed in this chamber 4 Display panel Displays measurement results and instructions 5 Operation panel See description on the next page 6 RS 232C connector Connects to a printer or computer 7 Connector for AC adapter Connects to the AC adapter 8 Battery charge LED This LED lamp is lit in red during charging of the battery y 9 and turns green when charging is finished Accessories AC adapter Use this AC adapter when operating the LUMITESTER from the AC line RS 232C cable Use this cable to connect to a printer or computer Contains utility software Operation Panel 1 lt POWER gt key 2 lt F gt key ON OFF kikkoman LUMITESTER G 110 3 lt Scroll gt key 4 lt ENTER gt key 1 lt POWER gt key Press this key to turn power on or off
33. Overview The LUMITESTER C 110 is a portable luminometer designed for simple and quick measurement of light emission by ATP Using a compact photomultiplier tube as the light detector the LUMITESTER C 110 measures low level light by photon counting method that ensures high sensitivity and low noise The LUMITESTER C 110 operates with either a rechargeable battery or AC adapter allowing wide flexibility with regard to measurement locations Operation is very simple All you need to begin measurement is just insert a sample tube and press the start key Measurement units are selectable from among RLU relative light units M pg and CELL The dedicated utility software allows easy creation and registration of a calibration curve data transfer etc Features High sensitivity Using a special reagent enables ATP measurement at 1x10 M 0 05 pg assay Note 1 e Wide measurement range ATP amounts can be measured over a wide range of 1x10 to 3x10 M Note 1 e Quick and simple measurement Just pressing one key starts measurement and the result is displayed in 10 seconds when in STANDARD mode e Data memory Up to 1000 data can be stored e ATP amount display ATP amounts M or pg can be displayed by pre registering a calibration curve The number of cells can also be displayed by registering the ATP amount per cell Calibration curve input The dedicated utility software allows creating and registering a calibration curve
34. The LUMITESTER is guaranteed for a period of one year from the date of delivery If any failure is found in the workmanship or materials within this warranty period we will repair or replace the defective parts without charge However the warranty does not cover any of the following cases even if within the warranty period The product has been misused not following the instruction manual It includes for example such case where the failure was caused by the use with an incorrect adapter or battery The product has been modified The failure was caused by natural or man made disasters including but not limited to earthquake fire lightning flood and others This warranty is limited to repair or replacement e Regardless of whether there is a failure in this device or not the warranty does not cover any loss of data caused as the result of the repair work or that the memory data or parameters are not indicated or not loaded to PC The scope of indemnification for failure of this device is limited to the indemnification provided by this warranty section The indemnification of any other loss or damage including but not limited to indirect damages such as lost profit any loss or damage caused as the result of the malfunction of the device or loss or damage caused under special circumstances etc is not included in the scope of indemnification Repair After the warranty period has expired the repair and replacement cos
35. 2 lt F gt key Press this key to enter the function routine mode 3 lt Scroll gt key Use this key to scroll the measurement data or select test conditions 4 lt ENTER gt key Press this key to begin measurement or enable data entry Power lt LUMITESTER operation gt The LUMITESTER can operate either from the rechargeable battery or AC adapter lt Battery charge condition gt The battery is charged by the AC adapter and the LED indicator changes during charging as follows Red during charging Green when charging is finished lt Charging time gt The charging time is about 4 hours lt To connect the AC adapter gt Just plug the AC adapter into the connector marked DC IN located on the side of the case When AC adaptor still continues to be plugged in AC outlet after charging is finished power continues to be supplied from AC adaptor lt Precautions when not using this unit gt For safety use unplug the AC adapter from the AC outlet when not using the LUMITESTER lt When a battery mark appears on the display panel gt The remaining battery power is low so recharge the battery If the unit which showed a battery mark is continuously used without charging with AC adaptor the battery may become in the state of overdischarge and a battery life may become short lt CAUTION gt Always use the AC adapter supplied with the LUMITESTER If an AC adapter other than supplied is us
36. gt key to turn the power off If you attempt to turn off the power while a sample tube is still in the LUMITESTER a beep sounds so open the cover and remove the sample tube Data Memory Measurement data is sequentially stored in memory as sample No 0001 to No 1000 When data for sample No 1000 is stored the next data is stored in memory as sample No 0001 All data stored in memory is backed up by battery and will not be lost even when the power is turned off When measurement is started with MONITOR mode turned on the data previously stored in memory will be erased and the measurement data written in memory from No 0001 so use caution To see the data previously measured use the lt SCROLL gt keys lt lt Data Scroll gt gt When scrolling with the lt v gt key the display stops at the latest data When scrolling with the lt gt key the display does not stop but continues scrolling until the lt gt key is released When any sample number is displayed pressing the lt ENTER gt key starts measurement and the measurement data is stored in memory as the next sample number for the latest data 5 1 2 ADVANCED mode measurement 1 On the MAIN MENU screen use the scroll keys to select the ADVANCED mode and press the lt ENTER gt key To set the measurement conditions refer to 10 Measurement time setting and 11 MONITOR mode in section 5 2 Function Routines 2 Prepare a tube containin
37. pg CELL ENTER ATP CELL ATP CELL pg 56 ATP CELL ATP CELL M AE 5 ENTER A 1 00E 18mol 6 E OO amol aa ENTER 57 ATP CELL ATP CELL M AE ATP CELL ENTER
38. BB CHEE PARE DEC OPEIRMHVET oH oe J Ax 41 Bo gt hi SE one RE en aa 43 PE h RTE EE Sele dect 43 3 ee 44 4 45 5 ei 46 5 1 ppp 46 5 2 ee 49 DE 50 ee ee eee ee eee ey 52 RS 232C eee eee eee eee 53 as tks ata os usd sesuch ahasiueataAksseesgeaea 54 ewes ees a cee aaa oan 56 ATP CELL bs eee et nerfeeeeerease aang 57 ATP CELL ee ee eee ee eee 58 pg 59 60 ee 61 Ce rate erent taht 62 6 i 63 7 ee eee ee 65 8 ttre 66 8 1 A SOS 7 esas sande 66 8 2 tt 66 Ge ER Be oat es tik ER SS sp eee 67 10 en 68 69 42 1 C 110 ATP
39. Data format used is a CSV format Refer to the computer operation manual for related computer cable connections and operation After cable connections and computer settings are complete execute the TRANS ALL DATA function available in the Function Routine menu from the LUMITESTER By connecting the computer to the LUMITESTER before starting measurement the data will be transferred to the computer when the measurement is complete If your PC does not have a serial connector then use a USB serial conversion cable USB RS232C conversion cable to connect to the USB port on your PC Recommended cable Make a choice between and USB serial conversion adapter Model SRC06USM SRCO06USB Manufacturer Buffalo Kokuyo Supply Inc RS232C straight cable 1 5m Commercial USB serial conversion adapter which is suitable for your OS Caution Windows7 has two different OS 32 bit version 64 bit version Please make sure your OS version before you choose an adapter 8 Maintenance AN WARNING Never wipe or wash the LUMITESTER with water Water may cause electrical shock fire or malfunction 8 1 Cleaning the measurement chamber If liquid spills in the measurement chamber clean as described below Spring e Turn the power off e Remove the cap on the bottom of the O ring measurement chamber and take out MSE Glass the inside parts as shown MS Wipe the spilled area with a
40. Doing so may allow penetration of the strong light into the inside of the unit and may damage the photomultiplier tube MEASURING 3 Start measurement XXXX e Press the lt ENTER gt key e The message shown at the right appears and measurement will start 10 11 e Wait until measurement is over until the center digit counts down to 0 lt CAUTION gt Do not open the cover during measurement e After 10 seconds the measurement results are displayed as shown at the right e When a printer is connected the measurement results are XXX XXX RLU also printed out e In units other than RLU the measurement results below the BLANK value are displayed as ND Not Detected The BLANK value is the amount of light emission obtained when a sample with zero concentrations was measured e If the message Scale Over appears this means the light level emitted from the sample is too high exceeding the upper limit of the LUMITESTER measurement range READY XXXX lt lt Scale Over gt gt The upper limit of LUMITESTER measurement range is 999 999 RLU approximately 100 000 pg in ATP measurement 4 Take the sample tube out of the chamber e To prevent carrying the LUMITESTER with a tube containing a sample fluid still installed in the measurement chamber make it a habit to remove the sample tube after measurement e To continue measurement repeat steps 2 to 4 5 Turn the power off e Press the lt POWER
41. ENU 22 6 ATP CELL coefficient selection CHOOSE ATP CELL Use this function to select the coefficient for converting the M mol per liter unit to the CELL number of cells unit Conversion coefficient type of cell can be selected from among 5 types A to E CHOOSE ATP CELL Procedure f A 1 00E 18mol e Select the type of cell using the scroll keys B 1 00E 18mol e Press the lt ENTER gt key C 1 OOE 18mol SCROLL The calculation formula for finding the number of cells is ENTER shown in Chapter 6 Relation between Each Unit MAIN MENU 23 7 ATP CELL coefficient input INPUT ATP CELL Use this function to enter the coefficient for converting the M mol per liter unit to the CELL number of cells unit INPUT ATP CELL Procedure e Use the scroll keys to select the type of cell from among A 1 00E 18mol Ato E e Press the lt ENTER gt key and the cursor moves to the edit position e Change the value using the scroll keys ARE YOU SURE e Press the lt F gt key and a confirmation message appears YES ENTER NO FUNCTION e Press the lt ENTER gt key to enable the change and return to the MAIN MENU MAIN MENU Input range 1 00E 20 mol to 1 00E 12 mol If the value you have entered is outside the input range the cursor moves back to that position so re enter the correct value 24 8 pg conversion coefficient input INPUT COEF pg Use this function to set
42. LECT NUMBER NUMBER OOOO0 0000 ARE YOU SURE YES ENTER NO FUNCTION PRINTING DATA STOP FUNCTION MAIN MENU 2 Data clear CLEAR ALL DATA Use this function to clear all data stored in memory Procedure e In the CLEAR ALL DATA function press the lt ENTER gt PRINT OUT key to clear the data CLEAR ALL DATA TRANS ALL DATA SET UP e To cancel data clear press the lt F gt key CLEAR ALL DATA WITH ENTER KEY MAIN MENU 3 RS 232C transmission TRANS ALL DATA Use this function to transmit all data stored in memory through the RS 232C port Procedure e In the TRANS ALL DATA function select the data you want to transmit and press the lt ENTER gt key TRANS ALL DATA e To stop transmission press the lt F gt key opel CAL CURVE For details on RS 232C refer to Chapter 7 Computer Interface TRANSFER STOP FUNCTION MAIN MENU 4 Various settings SET UP In the SET UP function you can make the following BEEP settings LANGUAGE DATE amp TIME COUNTRY BEEP This selects whether the BEEP sound is to be ON or OFF Procedure e Select ON or OFF using the scroll keys e Press the lt ENTER gt key Beep is sounded at start up and for alarm regardless of the OFF setting of BEEP MAIN MENU LANGUAGE This selects the display language Japanese or English Ep Procedure LANGUAGE r DATE amp TIME e Select the language using the scroll keys COUNTRY
43. RA LORE PROBST BENMGHVES fe A DP eO gt ER J Ax 40 e 10 50 C 20 95 e e e
44. ace this unit in locations where the temperature is extremely low or near heaters e Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan e Do not place any object on this unit e Be careful to prevent electrostatic charge buildup on the sample tube Static electricity may cause an increase the measurement data Precautions when using reagents CAUTION gt gt k ke Wear gloves and protective glasses and mask as needed and provide sufficient ventilation when handling the reagent Flying droplets from reagents or samples may cause skin or eye irritation or injury If an irritant reagent comes in contact with the skin or eyes wash thoroughly with tap water and consult a physician or pharmacist Precaution during installation or storage gt WARNING PLPL Do not use chemicals that may generate flammable gases and do not store this unit in an atmosphere containing flammable gases Doing so may cause this unit to explode internally Do not store this unit in locations where water or chemicals may penetrate inside this unit A short circuit may occur if water or chemicals penetrate inside this unit causing fire or electrical shock Other precautions during storage When storing this unit take the following precautions e Store this unit at temperatures from 10 to 50 C and a humidity from 20 to 95 e Do
45. cloth moistened with alcohol Put the parts you removed back into the measurement chamber in the reverse order and put the cap back on 8 2 Replacing the rechargeable battery pack If the battery power quickly runs out even after being fully charged it is a time to replace the battery pack If the characters on LCD fade out during measurement at level 3 or more of the battery mark the battery pack needs to be replaced e Turn the power off Cover e Remove the cover on the battery compartment and take out the battery pack e Unplug the battery connector then Battery pack replace the battery pack with a new Wire Red ong Q e Check with your dealer to make sure you buy the correct battery pack lt CAUTION gt 1 Always use the specified battery pack If other types of battery packs are used it may cause malfunction or may damage the unit 2 Please note the connector direction of battery pack when you insert it into the C 110 Refer to a left figure 32 9 Specifications 1x 10 to 3 x 10 M ATP LCD liquid crystal display panel Software is supplied for data transfer and calibration curve Software f creation registration Rechargeable battery or AC adapter 185W x 110D x 75H mm excluding projecting parts Weight Approx 700g Temperature Storage 10 to 50 C Operating 5 to 40 C A 33 10 Dimensions Dimensions are in millimeters 34 11 After Sales Service Warranty
46. d AN WARNING Q If an abnormal condition is found immediately turn off the power If operating the unit on battery power at that time then remove the battery If operating it from an AC adapter then turn off the power and unplug the AC adapter from the power socket If you notice abnormal operation or burning odor or smoke being emitted from this unit then there is a risk of potential fire or internal explosion After checking that the smoke has dissipated please contact us or our local dealer Never attempt to repair the problem yourself since this could be dangerous Continuous operation under abnormal conditions may lead to fire or electrical shock Precautions during use AN WARNING gt gt oO Do not use chemicals that may generate flammable gases and do not use in an atmosphere containing flammable gases Doing so may cause gas explosion in this unit Never attempt to remove any cover of this unit or disassemble or repair any part of this product Only our qualified service personnel are allowed to service this unit This unit contains high internal voltages so improper handling may cause electrical shock fire or abnormal operation Do not plug or unplug the AC adapter with wet or moist hands Doing so may cause electrical shock fire or malfunction When using the AC adapter always connect it to a power source ranging from AC 100V to 240V 50 60Hz Failure to follow t
47. e Press the lt ENTER gt key LANGUAGE JAPANESE ENGLISH MAIN MENU 20 DATE amp TIME This allows you to change the current date settings BEEP LANGUAGE Procedure DATE amp TIME e Press the lt ENTER gt key to move the cursor to where COUNTRY you want to make settings e Change the date and or time using the scroll keys e Press the lt F gt key DATE amp TIME e When a confirmation message appears press the lt ENTER gt key to enable the change and return to DATE OO OO OO the MAIN MENU HME 08 ARE YOU SURE YES ENTER NO FUNCTION MAIN MENU COUNTRY This allows you to select the date display format from the BEEP following three styles LANGUAGE JAPAN yy mm dd DATE amp TIME USA mm dd yy COUNTRY EUROPE dd mm yy COUNTRY Operation JAPAN e Select the country using the scroll keys USA Press the lt ENTER gt key EUROPE MAIN MENU 21 5 Display unit selection CHOOSE A UNIT Use this function to select measurement units Displays the relative amount of light that was measured RLU Relative Light Unit Displays ATP concentrations that were calibrated using a M mol l ea a calibration curve pg picogram Displays values that were converted to weight CELL Displays values that were converted to the number of cells Procedure Select the desired unit and press the lt ENTER gt key i CHOOSE A UNIT CHOOSE ATP CELL INPUT ATP CELL INPUT COEF pg MAIN M
48. ed it may cause malfunction of the LUMITESTER 5 Operating Procedure 5 1 Basic operation This section explains basic operations of the LUMITESTER C 110 The LUMITESTER C 110 can be operated in the following two modes for making measurements e STANDARD mode Measurement results are displayed in units of RLU relative light unit M pg or CELL which are selectable Measurement time is fixed to 10 seconds e ADVANCED mode This mode allows start delay time setting measurement time setting and ON OFF switching of real time count value display Measurement results are displayed only in RLU units 5 1 1 STANDARD mode measurement 1 Turn the power on e Hold down the lt POWER gt key until the message at the right appears KIKKOMAN e After 2 seconds the current date time and remaining LUMITESTER battery level are displayed Ver 5 0 The remaining battery level is shown in 1 to 5 steps with step 5 indicating fully charged The remaining battery level is not shown when the AC l MENU adapter is connected STANDARD MODE e The MAIN MENU screen then appears ADVANCED MODE e Check that STANDARD mode shows an asterisk and press the lt ENTER gt key 2 Prepare a tube containing the sample fluid e Open the measurement chamber cover with your finger e Put the sample tube into the measurement chamber e Press down the cover to close it lt CAUTION gt Don t push down the sample tube when the cover is open
49. g the sample fluid e Open the measurement chamber cover with your finger e Put the sample tube into the measurement chamber e Press down the cover to close it lt CAUTION gt Don t push down the sample tube when the cover is open Doing so may allow penetration of the strong light into the inside of the unit and may damage the photomultiplier tube 3 Start measurement e Press the lt ENTER gt key Measurement starts under the start delay and measurement time conditions which are set by MEASURING TIME function routine e Wait until measurement is over until the center digit counts MEASURING down to 0 XXXX 5 When MONITOR mode is ON the count value is displayed at specified time intervals GATE TIME e Measurement results are then displayed as shown at the i READY right In ADVANCED mode the measurement data is always EAA displayed in RLU units regardless of which measurement unit is selected XXX XXX RLU 4 Take the sample tube out of the chamber e To continue measurement repeat steps 2 to 4 5 Turn the power off e Press the lt POWER gt key to turn the power off If you attempt to turn off the power while a sample tube is still in the LUMITESTER a beep sounds so open the cover and remove the sample tube 5 2 Function routines The LUMITESTER uses function routines to clear data or make various settings First of all familiarize yourself with the basic operation flow for making conditi
50. his instruction may cause electrical shock or fire or damage this unit Use the specified AC adapter or battery that comes with this unit Using a different AC adapter or battery may cause electrical shock or fire or damage this unit Never connect an AC power source to the AC adapter input and RS 232C terminal Doing so may cause electrical shock fire or malfunction Do not expose this unit to water and do not handle it with wet hands This unit is not water proof so water or moisture may cause electrical shock fire or malfunction Other precautions during use Take the following precautions when using this unit e Do not move this unit during measurement This may cause fluctuations in the measurement data e Do not tilt this unit during measurement This may cause fluctuations in the measurement data e Use this unit while placed on a level surface e After measurement always take the measurement container out of this unit If this unit is moved or inverted after measurement while the measurement container is left inside then the remaining reagent may contaminate the measurement section possibly causing malfunction e Keep this unit at least 10 cm away from stirrers mixers or any device that may generate electromagnetic noise e Do not expose this unit to liquids reagents or organic solvents Doing so may cause malfunction discoloration or deformation If the unit was exposed to such liquids reage
51. mp TIME COUNTRY BEEP LANGUAGE DATE amp TIME COUNTRY LANGUAGE JAPANESE ENGLISH 54 DATE amp TIME BEEP Nae fea ran te B LANGUAGE ENTER EDATE ee TINE LET COUNTRY F DATE amp TIME DATE OO OO OO TIME_OO OO YES ENTER NO FUNCTION COUNTRY JAPAN yy mm dd USA mm dd yy EUROPE dd mm yy BEEP LANGUAGE 7 DATE amp TIME COUNTRY ENTER COUNTRY JAPAN USA EUROPE ENTER 55 RLU Relative Light Unit M mol l ATP
52. not store this unit in locations directly exposed to steam and in locations where condensation occurs e Do not store this unit in locations where corrosive gases are generated e Do not use this unit in locations subject to vibration e Do not use this unit in locations exposed to excessive dust or debris e Do not leave this unit in unstable or hazardous locations Do not apply excessive shock to this unit and do not drop it e Do not place this unit in locations where the temperature is extremely low or near heaters e Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan e Do not place any object on this unit When moving or transporting this unit When moving or transporting this unit take the following precautions Always use the original carton and packing materials when transporting this unit Our warranty does not cover any damage or breakdown caused during transportation due to use of non specified cartons and packing materials When moving this unit always first turn off the power and unplug the AC adapter from the power socket Disposal of this unit and reagents When disposing of this unit and reagents be sure to comply with local regulations regarding industrial waste disposal Consult the local regulations in your area for detailed information AN WARNING AN Do not place this unit in fire or attempt to burn it Doing so may cause the unit to ex
53. nts or solvents then wipe them away immediately e Do not press or rub the display panel or key operation section with hard or pointed objects Doing so may cause scratches or damages e Before connecting to the RS 232C terminal on this unit make sure the power is off e Before using this unit wash your hands thoroughly or wear sterilized gloves This unit is extremely sensitive so the ATP Adenosine Tri Phosphate may become contaminated through contamination from hands e Avoid conversation with others when you are using this unit This unit is extremely sensitive so the ATP may become contaminated through mouth saliva e Always use the specified instruments and tools e Use this unit at temperatures from 5 to 40 C and humidity from 20 to 85 e Do not use this unit in locations where the temperature fluctuates rapidly e Do not use this unit in location where exposed to strong light such as direct sunlight Doing so may cause damage of the photomultiplier tube or increase dark counts e Do not use this unit in locations directly exposed to steam or in locations where condensation occurs e Do not use this unit in locations where corrosive gases are generated e Do not use this unit in locations subject to vibration e Do not use this unit in locations exposed to excessive dust or debris e Do not leave this unit in unstable place or hazardous locations Do not apply excessive shock to this unit and do not drop it e Do not pl
54. on settings and selections LUMITESTER C 110 Menu List STANDARD MODE ADVANCED MODE FUNCTION ROUTINE PRINT OUT tea ALL CAL CURVE SELECT DATE SELECT NUMBER CLEAR ALL DATA TRANS ALL DATA DATA CAL CURVE SET UP BEEP LANGUAGE DATE amp TIME COUNTRY CHOOSE A al RLU M Pg CELL CHOOSE ATP CELL AtoE INPUT ATP CELL AtoE INPUT COEF pg INPUT CAL CURVE MEASURING TIME MONITOR MODE I GATE TIME OFF Press the lt F gt key to enter the function routine mode From the FUNCTION menu select the desired function and press the lt ENTER gt key When the selection and setting are complete the display returns to the MAIN MENU Pressing the lt F gt key on any screen returns to the MAIN MENU In some functions pressing the lt ENTER gt after pressing the lt F gt key determines the setting and then returns to the MAIN MENU Each function is described below 1 Print PRINT OUT Use this function to print out the stored measurement data or calibration curve Procedure In the PRINT OUT function select the item you want to print DATA Select this item to print measurement data CAL CURVE Select this item to print the currently set calibration curve After selecting the item to print press the lt ENTER gt key When you selected DATA further select the data to print from the following submenus ALL Prints all data stored in memory To abort printing p
55. plode internally Table of Contents T OVERVIEW a Weaken ea eed see cc ee en ee 7 2 gt Features Sacco NNR 7 3 Part Names and Functions siiicar a i ill needa 8 POWBTEEISNNN teva eae ey a E 10 5 Operating Procedure 15 11 ps BaSiC OPeSrallonmyeseectistendas E E EI s 11 5 1 1 STANDARD mode measurement RN 11 5 1 2 ADVANCED mode measurement pp 13 5 2 FUNCTION FOULM OCS aa e a e EE EE 15 PhinEIPBRINTGOUTLOSSK KM 16 2 Data clear CLEAR ALL DATA eccrine 18 3 RS 232C transmission TRANS ALL DATA 1 19 4 Various settings SET UP ieee 20 5 Display unit selection CHOOSE A UNIT pp 22 6 ATP CELL coefficient selection CHOOSE ATP CELL 23 7 ATP CELL coefficient input INPUT ATP CELL eect 24 8 pg conversion coefficient input INPUT COEF pg pp 25 9 Lat curve input INPUT CAL CURVE pp 26 10 Measurement time setting MEASURING TIME pp 27 11 Monitor mode MONITOR MODE pp 28 6 Relation between Each Unit 0 nl i iil ii iii ii nner 29 6 1 ATP concentrations LO 29 6 2 ALP amount pO a ieee CEES 30 6 3 Number of cells CELL aii 30 7 Computer Interface Ne 31 Ox MAINIGNANCE een eenen Wate eas eins ER i 32 8 1 Cleaning the measurement chamber Ne 32 8 2 Replacing the rechargeable battery pack pp 32 9 Specifications A SNR REESE SEG 33 TO DIMENSIONS 34 11 After Sales Service Ne 35 1 2
56. ress the lt F gt key SELECT DATE Prints all data of the specified measurement date Press the lt ENTER gt key and the cursor moves to the edit position Select the date using the scroll keys After selecting the date press the lt F gt key When a confirmation screen appears press the lt ENTER gt key to start printing To abort printing press the lt F gt key PRINT OUT CLEAR ALL DATA TRANS ALL DATA SET UP PRINT OUT DATA CAL CURVE PRINT OUT ALL SELECT DATE SELECT NUMBER ARE YOU SURE YES ENTER NO FUNCTION PRINTING DATA STOP FUNCTION MAIN MENU SELECT NUMBER Prints the data that is stored between the specified two data numbers Press the lt ENTER gt key and the cursor moves to the edit position Select the numbers using the scroll keys After selecting the numbers press the lt F gt key When a confirmation screen appears press the lt ENTER gt key to start printing To abort printing press the lt F gt key Input range The right hand data number must be equal to or larger than the left hand data number If the entry does not meet the above conditions or a data number where no data is stored is entered the cursor moves back to that position lt CAUTION gt Be sure to turn off the power when connecting or disconnecting a printer from the LIMITESTER PRINT OUT DATA CAL CURVE PRINT OUT ALL SELECT DATE SE
57. s elapsed or the data count has reached 1000 MONITOR mode is enabled only during ADVANCED mode measurement RAN MONITOR MODE e Select ON and press the lt ENTER gt key ON e Set the GATE TIME using the scroll keys OFF e After making the change press the lt F gt key e When a confirmation message appears press the ENTER lt ENTER gt key GATE TIME GATE TIME input range 1 Osec 0 5 to 10 seconds From 1 to 10 seconds the time is set in 1 second steps Measurement data display when MONITOR mode is ON ARE YOU SURE When MONITOR mode is ON the accumulated count re REF ae YES ENTER value within the GATE TIME is displayed This is not the NO FUNCTION count rate ENTER ADVANCED MODE MENU 28 6 Relation between Each Unit 6 1 ATP concentrations Conversion Formula calibration curve Relative light unit RLU ee ATP concentration M Calibration curve y ax b x Relative light unit RLU y ATP concentration M Relative light unit RLU can be converted to ATP concentration by entering a and b beforehand The default values for a and b are as follows Default value a 2x103 b 0 The relation between RLU relative light unit and ATP concentration is as follows Slope a is obtained by measuring standard ATP solution b is the blank light level represented in ATP concentration Calibration Curve ATP concentration M Relative light unit RLU 29 6 2 6 3 ATP amo
58. the coefficient for converting the M mol per liter unit to the pg picogram unit Procedure INPUT COEF pg e Press the lt ENTER gt key and the cursor moves to the edit position e Change the value using the scroll keys e Press the lt F gt key and a confirmation message 5 O7E 10 appears ARE YOU SURE e Press the ENTER key to enable the change and return to the MAIN MENU YES ENTER NO FUNCTION Input range MAIN MENU 1 00E 05 to 1 00E 20 If the value you have entered is outside the input range the cursor moves back to that position so re enter the correct value 25 9 Calibration curve input INPUT CAL CURVE Use this command to set a calibration curve INPUT CAL CURVE Procedure mee y ax e Change the values using the scroll keys a 1 00E 12 e Press the lt ENTER gt key and the cursor moves to the b 0 00E 00M next edit position e After making the changes press the lt F gt key e When a confirmation message appears press the ARE YOU SURE lt ENTER gt key to enable the changes YES ENTER NO FUNCTION MAIN MENU Input range a 1 00E 17 to 1 00E 10 b 1 00E 08M to 0 00E 00M If the value you have entered is outside the input range the cursor moves back to that position so re enter the correct value 26 10 Measurement time setting MEASURING TIME Set the measurement time and start delay time to make measurements in ADVANCED mode
59. ts required to recover the original functions will be charged to the user If you suspect a failure which may require repair contact our sales dealer providing the production serial number and a detailed description of the problem While every effort will be made to repair the returned product in as short a time as possible the repair in the following cases may require additional time unusual cost or may be refused 1 The product was purchased long time ago 2 The product uses maintenance parts which are not in current production 3 The product was severely damaged 4 The product was modified 5 Problems cannot be observed by us 6 Other similar cases Contact information Kikkoman Biochemifa Company 2 1 1 Nishi Shinbashi Minato ku Tokyo 105 0003 Japan TEL 81 3 5521 5490 FAX 81 3 5521 5498 E mail biochemifa mail kikkoman co jp URL http biochemifa kikkoman co jp e 35 C 110 C 110 36 Ver 5 2 K N RAAZ
60. unt pg Conversion Coefficient ATP concentration M ATP amount pg ATP amount pg ATP concentration M x conversion coefficient pg coefficient The conversion coefficient default value is 5 07x10 507x107x10 507 ATP molecular weight 10 100 microliter liter When the sample liquid amount is 100 microliters the default value can be used as is Number of cells CELL Conversion Coefficient ATP concentration M Number of cells CELL ATP concentration M Number of cells per milliliter x 10 ATP mol CELL 30 7 Computer Interface The LUMITESTER C 110 is supplied with computer software LUMITESTER C 110 Utility Software This software allows you to easily create and register a calibration curve and to perform data transfer See Utility Software Operation Manual for detailed information This section explains how to transfer data to the computer without using the utility software Measurement data and registered calibration curve data can be transferred Remote control and data transfer cannot be performed from the computer To connect the LUMITESTER to the computer use the RS 232C cable supplied with the LUMITESTER Set the computer protocol so that it matches the LUMITESTER communicating conditions listed below RS 232C Communication Conditions Baud rate bit sec 19200 bps Data bit Parity check Yes Even parity 1 Stop bit Flow control Hardware
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