Blend Taq －Plus - 東洋紡ライフサイエンス事業部
Contents
1. TaqDNAPolymerase PCR PCR PCR Taq PCR 1 Blend Taq Plus 2 BTQ 201 BTQ 201X5 250U x 1 Blend Taq Plus 2 5U l 100lx 1 10x Buffer 1000glx1 BTQ 201 x5 2mM dNTPs 1000 lx 1 PCR Mg 2mM 2 gt gt 3 Blend Tadg Plus human globin 17 5kb gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 002. 3 10kb dNTPs 0 3 0 4mM 4 Blend Taq Plus PCR TAcloning vector 1kb PCR 2 gt TOYOBO mf 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 6 human 8 globin 3 6kb Blend Tag Plus _ A human B globin 3 6Kb 1 3 4 3 2 5 Blend Taq Plus A Blend Taq Plus 5ng A Lane1 0ng human genome DNA Lane2 5nghuman genome DNA Lane3 10ng human genome DNA
3. A3666K E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 4 PCR 1 PCR ComponentS Volume Final Concentration 10x PCR Buffer for Blend Taq 5 ul 1x Blend Taq Plus 2 5U gl 0 5 ul 125U reaction 1 50 ng Plasmid 2 ii 10 1000 ng Genome 10pmol gl 1 0 2M each 2mM dNTPs 5 ul 0 2mM Autoclaved distlled water to 50 ul 2 PCR Narbero cyces 94C 2min 4C 2 Denaturation 30sec Primer Tm 5 C 55 60 C 30sec 68 C 0 7 C 7 C 1minykb PCR target nsion 1min 1min kb PCR target 3 Annealing 5 1 Denaturation time 94 25 30sec 98C 510sec 2 6kb PCR Tm 70 C
4. 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio
5. Lane 4 20ng human genome DNA Lane5 40ng human genome DNA http wwwr toyobo cojp bio PC12 RT PCR PCR ES RT PCR PCR Blend Taq Plus PCR PCR Blend Taq Blend Tad Plus gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URLI http www toyobo co jp bio 7 l
6. 14 03 Code No BI Q 201 Code No BI Q 201X5 20C Hot Start PCR Taqg Blend Tagqg PlIus 3 5 Proof reading Taq DNA polymerase pol I Proof reading o Blend Long PCR Blend Taq PCR Taq DNA polymerase w Blend Blend Taq Plus Taq anti Taq highn PCR Polymerase PCR PCR DNA PCR TaqDNApolymerase PCR
7. t Taq gt 250 U x 1 BTQ 101 Blend Tad 250Ux 1 x5 BTQ 101X5 250 Ux 1 x10 BTQ 101X10 Blend Taq Blend Taq Plus Buffer BTQ 1B lt ReverTra Ace cDNA gt everTra Ace c 100 a Rever Tra Ace lt TA gt 10 TAK 101 TArget Clone 5 lt Taq gt 100 lx1 TCP 101 ant Tad high 100 lx1 x5 TCP 101X5 lt PCR gt 200 NPK 601 MagExtractor PCR amp Gel Clean up dNTPs Mixture 2mM NTP 201 8 1 Bames W M Proc Natl Acad SoiUSA 91 2216 2220 1994 TOYOBO 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order lifescience toyobo jp gt TOYOBO mf 9 00
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