取扱説明書
Contents
1. 14 PCR 90 slope lt 3 6 PCR 4 2 PCR PCR Tm 60 C Tm2. DNA 16 7 PCR 1ml x 1 40 QPS 101T PCR 2 THUNDERBIRD Probe qPCR Mix 0 QPS 101L THUNDERBIRD Probe qPCR Mix 50X ROX reference dye
3. HPLC OPC 3 DNA DNA cDNA genomic DNA plasmid DNA virus DNA DNA a cDNA cDNA PCR 10 PCR PCR
4. 50X ROX reference dye 50X ROX reference dye SYBR Green 4 1 50X ROX reference dye PCR 45 60
5. 15 no template control NTC no template control 41 2 PCR PCR HPLC no template control
6. 4 1 HIP 20 C 2 8 C 20 C ROX reference dye 50X ROX reference dye THUNDERBIRD SYBR qPCR Mix THUNDERBIRD SYBR qPCR Mix ROX 4 ROX 1X qPCR Mix 50X ROX 5ml 200ul QPS 201L 1 67ml 66 8ul QPS 201 1ml 40ul QPS 201T ROX 0 1X qPCR Mix 50X ROX 5ml 20u QPS 201L 1 67ml 6 7ul QPS 201 1ml 4ul QPS 201T De 3
7. DNA DNA PCR DNA PCR DNA DNA DNA Yeast RNA
8. http www toyobo co jp bio 17 HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience toyobo Jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
9. p 7 3 3 PCR p 12 PCR PCR Ct ct PCR Ct PCR 110 slope gt 3 1 PCR Ct ct PCR Ct PCR
10. Setup Instrumert Mode Fast Standard 9600 Emulation Sample wolume HL Bs Thermal Cycler Protocol Thermal Profile auto Increment Ramp Rate Data Collection Stage 1 Stage 2 Stade 3 Repeats lo add Cyclg dd Hold Add Step Delete Step Add Dissociation Stage 2 Data Collection Setup InStrumert Thermal Cycler Protocol Mode Fast Standard 9600 Emulation Sample wolume HL Es Thermal Proflle Auto Incremert Ramp Rate Data Collection Stage 1 Stage 2 Stage 3 Repeats 40 950 950 60 0 60 0 95 0 95 0 EN TN EE EN 3 2 2 Roche LightCycler 1 1 3 5 Roche LightCycler 1 1 1 Analysis Mode None
11. SYBR Y Green Realtime PCR Master Mix Code No QPK 201 SYBRY Green Realtime PCR Master Mix Plus Code No QPK 212 gt PCR PCR 1 PCR 2 PCR 3 4
12. 45 60 30 Applied Biosystems 7000 7300 31 7500 35 5 12 5 RNA cDNA cDNA PCR ReverTra Ace qPCR RT Kit Code FSQ 101 PCR cDNA ReverTra Ace qPCR RT Kit PCR cDNA
13. 1 PCR PCR 2 20 lt 30mer GC 40 60 200bp 80 150bp DNA melting temperature Tm 60 65 C
14. 1 RNA RNA 65 C 5 RNA 5x RT Buffer RNA 2 Nuclease free Water XHI 5x RT Buffer 2 HI RT Enzyme Mix 0 5 Hl Primer Mix 0 5 Hl RNA 0 5pg 1pg Total volume 10 Hl 3 37 C 15 98 C 5 4 C 20 C PCR
15. RNA 13 6 DNA SYBR Green DNA SYBR Green DNA Ct PCR PCR cDNA
16. PCR DNA DNA DNA DNA DNA DNA
17. plasmid DNA DNA DNA DNA PCR yeast RNA plasmid DNA 1ug plasmid DNA 9 1 x 10 plasmid DNA kb 4 4 1 50 ul 20 ul 50ul 20Ml XH XH THUNDERBIRD SYBR qPC
18. Cycle Program Data Analusis Mcde Quantification Cyclesl 1 Temperature Tarqets Target Temperature Incubation Timg hrs min sec Temperature Transition Rate CS Target Temperature O Step Size CO Step Delay Cycles Acduisition Mode 2 PCR Analysis Mode Quantification 60 C Acquisition Mode SINGLE Cycle Program Data Analysis MedelNone 4 Quantitication Gyeleal 40 Temperature Targets Target Temperature C Incubation Time hrs min sec Tamperature Transition Rate C7 s Target Temperature C Step Size Step Delay cycles Acquisition Mode Ep mo EEC 0 ore 10 3 Analysis Mode Melting Curves 95 C Temperature Transition Rate 0 20 Acquisition Mode CONT Cycle Program Data a Cucles 1 uantification_ velting Curyes Temperature Tarqets Tarqet Tamperature CI Incubatinn Time hrs min sec
19. 109 PCR ReverTra Ace qPCR RT Kit Code No FSQ 101 PCR 20 b 9enomic DNA virus DNA genomic DNA virus DNA SYBR Green 2 DNA 2 DNA DNA SYBR Green lt genomic DNA 50ul 200ng c plasmid DNA plasmid DNA
20. SYBR Green Realtime PCR Master Mix Code No QPK 201 SYBR Green Realtime PCR Master Mix Plus Code No QPK 212 gt ROX 1X 2 PCR p 3 2 PCR 2 PCR Tm 60 C 3 PCR 2 3 3 PCR 95 C 20 lt 60 PCR 95 C 1 15 40cycles 60 C 30 60 Data Collection Melting Dissocia
21. 15 3 PCR PCR Applied Biosystems 3 Applied Biosystems 7500Fast Roche LightCycler 1 x 2 0 Applied Biosystems 7700 7500 7900HT StepOne M StepOnePlus W Agilent Technologies BioFlux Line Gene 5 15 3 56 64 C 4 300bp 30 30
22. 45 60 30 Applied Biosystems 7000 7300 31 7500 35 5 2 1 Applied Biosystems 7900HT 2 2 2 Applied Biosystems 7900HT 1 Instrument Thermal Profile Add Dissociation Stage Sample Volume uL 45
23. PCR 95 C 20 60 PCR 95 C 1 15 40 cycles 55 65 C 5 30 206 30 60 Data Collection Melting Dissociation Curve Analysis 1 PCR p 6 2 Tm Tm 5 C 3 5 15 30 4 300bp 30 30
24. 50ul 1000 QPS 101X5 QPS 101 5 QPS 101 cDNA na PCR cDNA ReverTra Ace qPCR RT Kit 200 FSQ 101 PCR cDNA ReverTra Ace qPCR RT Master Mix 200 FSQ 201 PCR cDNA DNA ReverTra Acee qPCR RT Master Mix 200 FSQ 301 with gDNA Remover 1 PCR QRT 101T 1 PCR MA g77ecf Realtime PCR Master Mix QRT 101 SYBRY Green 0 5ml x 2 1 PCR 40 EOL RNA direct SYBR Green 0 5ml x 5 ORT 201 Realtime PCR Master Mix 100 A MA4 g7ecf 50mM Mn OAc 50ul 500 QRT 101X5 QRT 201X5 QRT 101 QRT 201 5
25. 50X ROX reference dye Applied Biosystems Agilent Technologies ROX 5 Taq lt SYBRS Green gt SYBR Green DNA SYBR Green PCR 2 FHS QPS 201 QPS 201L QPS 201T 20 C THUNDERBIRD 2 8 C 5ml 5ml 1ml SYBR qPCR Mix 3
26. 1 67ml x 3 5ml x 1 1ml x 1 20 C 50X 2 8 C 250H 250 Hl 50hl ROX reference dye THUNDERBIRD SYBR qPCR Mix SYBR Green dNTPs Mg rTaq DNA DNA 2X DNA cDNA genomic DNA plasmid DNA 1X ROX passive reference dye 20 C 3 2 8 C 20 C 10 SYBRY Green 50X ROX reference dye Applied Biosystems
27. Tamperature Transtion Rate s Secondary Target Tamperature I Step Size Step Delay Cycles cdulsitinn Mode 5 ihs Weoon re INONE 5 on 0 NONE ET ICONT 4 Analysis Mode None Cycle Program Data Analusis Mcde a Quantification Cyclesl 1 Temperature Targets Target Temperature CI Incubation Time hrs min sec Temperature Transition Rate C7 s Tarqet Temperature CI Steh Size CO Step Dalay Cycles cdulsitinn kode HO Bo pooo NONE Cs 5 11 2 3 3 PCR p 6 2 PCR 3 PCR Tm 60 C 300bp
28. R Mix 10 Hl Forward Primer 6 pmol 0 3 uM Reverse Primer 6 pmol 0 3 uM 50X ROX reference dye 0 470 04ul 1x 0 1x DNA Y HI 20 HI 1 0 2 0 6 nM 2 50X ROX reference dye Applied Biosystems 1 ROX reference dye Applied Biosystems 7000 7300 7700 7900HT StepOne 1X 1750 StepOnePlus Applied Biosystems 7500 7500Fast Agilent Technologies ee Roche Bio Rad BioFlux Line Gene
29. TOYOBO ldeas amp Chemistry THUNDERBIRD SYBR qPCR Mix Code No QPS 201 QPS 2011 QPS 201L TOYOBO CO LTD Life Science Department OSAKA JAPAN A4196K 1 2 3 4 5 RNA cDNA 6 7 JE LightCycler Idaho Technology Inc Roche Molecular Systems Inc SYBR Molecular Probes Inc 1 THUNDERBIRD SYBR qPCR Mix SYBR Green PCR 2X ROX
30. tion Curve Analysis 1 DNA 60 2 Applied Biosystems 20 Applied Biosystems 7500Fast 9 30 TM Roche LightCycler 1 x 2 0 Applied Biosystems 7700 7500 7900HT 60 StepOne StepOnePlus W Agilent Technologies BioFlux Line Gene 2 PCR PCR
Download Pdf Manuals
Related Search