IO テスト PE 標識抗体 CD5 IOT1a
Contents
1. IO2. 2 296 EPICS XL vs PE Lumphocutes Leukncutes 8 2 Count 4 9 TROL c CD45 FITC C A07738 IM1201 IOTest T B NK
3. 2 3 4 5 6 7 8 IO PE CD5 IOT1a 2 8 Cc 12 IO PE CD5 IOT1a A07753 100 2mL 1 Rothe G Schmitz G Adorf D Barlage S
4. 3 6 4 1 _8 A09777 I IM3648 VersaLyse IM1400 OptiLyse B A11895 I A11894 OptiLyseC A07799 I IM3514 IOTest 3 4
5. 0 5 ersaLyse PBS 1mL st 3 A07800 I IM3515 12 5 L 0 19 2 PBS a PBS 1mL 3 A07800 IM3515 67 5u 0 59 PBS B M B CLL CD5 B CD72 CD5 CD72 B BL1a IgG2a PE Phycoerythrin 486 60 lt 590nm h 1 IO 2 EDTA
6. HLA DR KM3 1 Raii 2 95 HLA DR JURKAT 3 0 5 1 KM3 Non T Non B Leukemia 2 Raji Burkitt lymphoma 3 JURKAT T cell Leukemia PBS 4 HLA DR KM3 Raii 3 10 3 5 5 1 0 196
7. 1 12mm x 2 Ficoll Paquei 5x10 mm 3 05 IOTest Mouse 00 xg 5 24 FS 90 SS PE Log
8. IOTest Mouse lgG2a PE A09142 A09141 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100L 5x10 mm 10x10 mm PBS 3 x 103 mm3 1 25C 500 x g 5 2 3 4 LH700 5 PBS 5 10 x 10
9. 7 Ficoll Padue 8 9 10 11 T 1 1 PBS PBS p
10. 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 xg 30 4 Ficoll Paque 5 PBS 2 8 C 400 x g 8 6 PBS 7 2 8 400 x g 4 8 PBS 9 2 8C 400 x g 3 10 PBS 5 x mm 5 x 10 mL 11 90
11. Bray R Gascoyne Picker L Stetler Stevenson M recommendations on the imm hematologic neoplasia b interpretation 1997 C 6 Stewart C C Behm F R E Hudnall S D Hurtub Wormsley S nadian consensus recommendations On lysis of hematologic neoplasia by flow as results of an international consensus meeting 2001 ry 46 23 27 V Angelisova P Comparatives biochemical studies on the Workshop CD5 and CD3 panel antibodies 1987 Leucocyte Typing Il White Cell Differentiation Antigens McMichael A J et al Eds Oxford University Press 197 9 Disanto J P Small T N Dupont B Flomenberg N Knowles R W Analysis of human CD8 and CD5 antigens expressed on mouse L lines 1987 Leucocyte yping Ill White Cell Differentiation Antigens McMichael A J et al Eds Oxford University Press 210 214 10 Reiter C Cluster report CD5 1989 Leucocyte Typing IV White Cell Differentiation Antigens W Knapp et al Eds Oxford University Press 331 332 Spits et al 1985 Eur J Immunol 15 88 91
12. 2006 3 2004 10 202EZY00111000 A07753 T IO PE CD5 IOT1a 1 2 3 4 IO PE CD5 IOT1a PE lgG 20L 1 CD5 67kD CD5 T 6
13. mm 1 12mm x 75mm 2 100L 3 20L IOTest Mouse lgG2 PE 20L 4 15 5 6 PBS 2mL 7 400 x g 3 8 7 8 9 0 5 1mL 0 1 0 59 PBS 10 24
14. Gramatzki M HOffkes H G Janossy G Knuchel R Ludwig W D Nebe 1 Nerl C Orfao A Serke S Sonnen R Tichelli A WOrmann B Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies 1996 Leukemia 10 877 895 2 Jenning C D Foon K A Recent advances in flow cytomet 105 0001 3 5 1 Immunotech a Beckman Coulter Company 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France 105 0001 Application to the diagnosis of hematologic malignancy Blood 8 90 2863 2892 3 Rosenberg S A Classification of lymphoid neopl Blood 5 84 1359 1360 4 Kilo M N Dorfman D M The utility of flow immunophenotypic analysis in the distincti lymphoma chronic lymphocytic leukemi lymphoma 1996 Am J Clin Path 5 Borowitz M J
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