粒径3μmイオン交換樹脂を用いる高速アミノ酸分析計の開発
Contents
1. 7 3 km2. D 2850 5 2RN w 4 Au hu RATIO he X100 100 RATIO 100
3. 3 1 L 8500 2 3 B 1 B5 V1 V5 1 30 70C 6 4 2 3 R V 6 V8
4. 1958 Spackman Stein Moore 1962 KLA 2 21 44 1977 835 50 4 L 8500 30 2 1 500mm 60mm 5nmol 10pmol 500 1
5. 3 2 1 L 8500 PH PE 2 L 8500 3 4 029SC P HH SC PF 2650L 4 6 mm 60mm 4 6 mm 40mm
6. 3 m 835 49 336 VOL 69 No 4 1987 4 Column 4 6mmdX60mm 2622SO Flow rate 0 4ml min Sample injection 20pmol 10gl 11 1 DO 0 Te 2 ge i A as 2 ss LL lt T gp 3 cn Gu lt gt oa bs Te as C 1 1 1 UMDM 9 W UMiEwUlersiEL LnMWIIWE Apw nnalwsini Te 9 2
7. 5 4 1 835 30pmol 8500 655A
8. 100 LA 2 37 44 m O KLA 3 Stein Moore 50 40um 30 20 KLA 3A 20 4m KLA 5 17 2 m mn 3 835 2 5um 8 _835 8500 8 3 1960 1970 1980 1990 334 VOL 69 No 4 987 4 835 5gum 8500 3zm
9. 1 300 1 000 4 000 4 4 39 GluNH Trp 8 Arg 110 CV 0 5 CV 2 0 i 3zm
10. 110 FLOW MONITORING DIAGRAM B 1 B2 B3 B4 B5 R1 AUTO SAMPLER COLUMN TEMP REACTION UNIT ANALYZING UNKNOWN PUMP 1 MAX LIMIT 2 L 8500 25 140 50 440 nm 570nm F 1100 D 2850 50 CYCLE TIME RUN IME R2 R3 LINK VIAL PROG FILE PUMP3 ml min DATA PROCESSOR kg cm2 lt DETECTOR DRAIN 3 CRT Cathode Ray Tube 46
11. U D C 543 544 6 54 466 06 5 112 3 3 zm High Performance Amino Acid Analyzer with 3 m lon Exchange Resin VM 9 Yoskhinort Takata 3 ka Yoshio Fu Hideo Takahashi Shigetake Ganno 3gum 30 2 3 6 10pmol 500fmol 16
12. 337 PROGHAM TABLE PROG No 6 REAGENT VALVE V6 V7 50 50 PRESSURE P1 MAX MIN 260 50 Kg cm P2 50 5 REACTION TEMP 120 Degrees C P3 50 0 STEP TIME BUFFER TOTAL 100 COLUMN FLOW RATE ml mn min Vi V2 V3 Vt VS TEMP P1 P2 P3 1 0 0 100 38 035 0 30 0 00 2 10 0 32 3 17 9 100 4 18 0 80 20 5 20 5 52 6 32 5 70 30 7 32 6 10 90 8 39 0 45 9 48 0 10 90 10 48 1 100 Te 11 51 0 100 70 7 12 51 1 100 13 67 0 45 9 14 74 0 100 3 15 74 1 ss CN 16 92 0 70 RS 17 107 0 100 gt 18 107 1 100 La 19 120 0 100 20 120 1 100 38 Oo 21 150 0 100 ee m LL_ lt LL So i lt 2 ete i 6 CN i 5 SL 09 5 C 92 a Oo wn wu ZS wm lt lt lt gt 3 3 CN lt s MN 4 4 EO EE TO ET ET ET TT ET ET TE DR RD FU tO CAT CU ET EN Te Te Le OO To AO Te Te c CN CN C ha C O Ee OO OO 8 110
13. 4mm 3mm 4 230kg cm 0 2 3zm 335 S_ 300 I ee es et 100 Eis 0 a ee MD 2 Pa ee 0 a 1 et 0 1 0 2 0 3 0 4 0 0S uf QC 0 09 10 ml min 4 0 2 4 2
14. 1 700 4 5 9 20pmol SN 3 200fmol 500fmol Hypro Pro Ala 8 AiBA Ans Car CV 0 6 835
15. N b 6 RATIO 00 10 48 Column 4 6ID X60mm 2622H Flow 0 4ml min 100kg cm Sample conc 2nmol 10I Std Analysis time 30min gt 4 3 oO wl 2 Fla gt i lt _ uo 5 es CRD 6 IN NN WS EO 0 0 Te i CN CN Retention time min 7 30 0 5 1 5 5 35C 1 90 110V 0 2 20nmol
16. CV 10 L 8500 Gly His CV 3 4 6 835 L 8500 835 10 3 m 2620 10 3 m 8500 30 2 3 6 61 2
17. 6 2 100 10 2 4 3 7 570nm 440nm 19 30 CV 47 336 VOL 69 No 4 I987 4 9 WP TO 2 CRISIS Lys His Tyr Arg AP Se NN WW ww 8 8 NAME Tyr 2 Phe 3 Lys 4 His 5 Trp 6 NH3 7 Arg No 5 a
18. 0pmol Column 4 6mmidX80mm 2620SC Flow rate 0 15ml mn Sample injection 200pmol 20 aa wc 0 gt a CN oe oa s LL QB gt LL CySH GS 250 ATT Pro Pe Cys NH3 1 CH 10 80mm 2620SC 1 D H Spackman et al Anal Chem 30 1190 1958 2 623 1976 3 64 1978 4 1502 1979 5 L 8500 1986 SI 29 4 18 1986
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