報告1
Contents
1. 6 8 ee 6 vyv 16 2 121C 152. 1 1 pcDNA3 1 2 EcoRI BamH1 DNA EGFP DNA Takara DNA Ligation DNA DNA DNA Competent cell EGFP 2 2
3. 4 4 m 5 5 pcDNA3 1 EGFP pcDNA3 1 4 5kbp EGFP 0 7kbp 2 3 ES ips
4. 37 2009 7 1 1 DNA DNA JST
5. 3 JST
6. 21 SPP 1 2006 21 2009 21 2009 4 Ecoli DH5 a Competent Cells 3 2009 3 2009 2005 H http www 1ifescience mext go jp bioethi cs anzen html 2010 2 9 2 3 6
7. Pl 7 2 1 20 JST 21 JST JST 1 3 2
8. 8
9. Competent cell LB LB 37 8 16 3 3 LB 2 LB LB 5 6 37 8 16 iv 3 QIAGEN QIAprep Spin Miniprep kit DNA Ecor I
10. 2 P1 21 12 8 10 3 10 3 i i 1
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