取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 15 MFX 2000 2100 5 000r p m RNA MFX 2000 2100 RNA 2 A260 2802. MFX 2000 2100 40ul 5 3 Code No MFX 401 Code No MFX 401 MFX 2000 2100
3. 1 5 13 31 2 7 14 33 3 9 15 35 4 11 16 37 5 13 17 39 6 15 18 41 7 17 19 43 8 19 20 45 9 21 21 47 10 23 22 49 11 25 23 51 12 27 24 53 1 6 13 33 2 8 14 35 3 10 15 37 4 12 16 39 5 14 17 41 6 16 18 43 7 18 19 45 8 20 20 47 9 22 21 49 10 24 22 51 11 26 23 53 12 28 24 55 6 4 Code No MFX 301 A E AE A E 6 Code No MFX 302 1 5ml RNA
4. 1 I 50ml 2 7 2ml 9 I 15m 10 7 I 50ml 50ml 15ml ml 6 3 4 1 24 1 5ml
5. 1 5ml 620 750ul 700 lt 750ul 620ul 600uIl 7
6. START 9 6 RNA 3 4 700ul 700ul 50ul 20sec 40 lt 60sec BF 3 600ul I 10sec B F 800ul 5sec B F 800ul 5sec B F 40Ml 5sec 65 C 2min 5sec
7. 15 6 RNA DNase I 0 C lt 1 Vogelstein B and Gillespie D 1979 Preparative and analytical purification of DNA from agarose Proc Natl Acad Sci 76 615 619 2 Boom R Sol C J A Salimans M M M Jansen C L
8. 1 4 2 100 1 vv 2 4 3 41 1 1 2 2ME
9. DNA mRNA RT PCR RNase free DNase I DNA 1 10xDNase I buffer RNase free DNase I 10U Ll DEPC TE 24 1 5M pH 2mg ml 709 2 DNase I RNA X pl DEPC 8 5 X 10x DNase I buffer 1 RNase free DNase I 10U pl 0 5 Total 10 hl 10 30min lt 10ul DNase I lt 100ul DEPC 100ul TE 5min 4 C 12 000r p m x5min
10. RNA RNA A260 280 RNA RNA A260 280 RNA 13 3 RT PCR DNA DNA 11 DNase RT PCR PCR DNA RNA 4 DNA 4 RNA RNA RNA
11. 10 gt 20 A260 RNA 3 000r p m 5sec 4 RNA ng Ul A260x40x 10 7 DNase I RT PCR mRNA mRNA Pseudogene
12. B F 2 65 RNA B F 40ul TRNA 620l Lysozyme RNA DNA 11 DNase 1 4 5 MFX 2000 2100 RNA MFX 2000 2100 MFX 2000 2100 MFX 2000 2100 RNA Code No MFX 102 Code No MFX 103 1
13. 0 5ml RNA Lysozyme RNA DNA 3 1 100 RNA 77ml 4 C 66ml 4C 176ml 4C 10ml RNase free 4C 6ml E 4C
14. MFX 2000 2100 5 8 1 3M pH5 2 7 1 25 2 5M 0 9 1 2
15. 2ME 750 lt 900ul 10 30mg 1 Mag cg 77apper Code No MGS 101 ME RNA 20 4 30 60 3 000 5 000r p m 10 620 750ul 21G
16. RNase 70 C DNase I DNase I RNA 11 DNase I DNase I DNase I 80C RNA RNA RNase 5
17. 1015 3 Zymolyase 20T 20 000U g Zymolyase buffer 30mg ml Zymolyase 2 50ul Zymolyase 50ul Zymolyase 37 5 20 700ul 2ME 30 10 15 4 MagExtractor MA4 2ME 10 15 1 RNA B F 1 10 B F I 5 2
18. ldeas amp Chemistry Nucleic Acid Purification Kit MagExtractor VA Code No NPK 201F TOYOBO CO LTD Life Science Department OSAKA JAPAN A4122K Zz 1 RNA RNA RNA RNA RT PCR PCR MFX 2
19. MFX 200072100 RNA 2 RNA 40Hul DNase 1 1 10 40ul 500ul RNA RNA RNA 1 10 21 RNA Normal 22 RNA Ethanol 2 65 C 10C
20. 2ME 77ml 770ul 2ME 2ME 4C 3 I 3 RNA 2ME 1 1 5ml 700ul 2ME RNA 30 10 15 2
21. 100mM Tris HCI 20mM MgCL pH 7 5 lt DNase I RNA 11 24 1 4C 12 000r p m x5min 100ul 5ul 2mg ml 100ul 5M 200ul 20 C 30 60min 4C 12 000r p m x10 15min 1ml 70 4C 12 000r p m x2min DEPC Negative control PCR RNA 12 5 1 RNA
22. Wertheim van Dillen P M E and van der Noordaa J 1990 Rapid and simple method for purification of nucleic acids J Clin Microbiol 28 495 503 15 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_ lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience toyobo Jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
23. 000 2100 MFX 2000 2100 MFX 2000 2100 RNA RNA rRNA mRNA RNA MFX 2000 2100 24 4 2 MagExtractor VM4 5x10scells 10hg 10scells RNA 30mg 15ug 30mg RNA 10 O D 660nm lt 20ug 5 O D molyase
24. RNA Zymolyase 20 000U g Zymolyase buffer 0 9M Sorbitol 0 1M EDTA 50mM DTT pH7 5 2 MFX 2000 2100 Code No MFX 301 Code No MFX 401 50ml 15ml 2ml 50ml BLUE MAX 2170 15ml BLUE MAX Jr 2196 BECTON DICKINSON 2ml 2220 2200 72 693 72 694 2 1 5ml 65C 3 000 5 000r p m 2 2ME 100 1 v v 1 700ul 7ul
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