等温増幅蛍光測定装置 Genie III 取扱説明書
Contents
1. OptiGene Manual2. 33 1 Genie II 1 1 PC 1 USB 1 1 2 Genie II 8 x 1 10 150 ul 4 PID 100C 470 nm excitation 510 560 nm emission 590 nm excitation gt 620 nm emission LCD 480x272
3. 13 6 PROFILE AssaysjLamp amp Anneal prof DR Next 14 7 Profile ig LOGIC Tam C
4. Genie III Genie I USB 3 Genie II Battery Statuls Chargng 3397 mAh 16 6V 1 0 2h 10min actve hrre Genie III
5. 1 Genie II LED 2 s LAMP amp Ameal kcn IAnneal 9358 Other omer 3
6. 17 IV PC 1 Genie Explorer Genie I PC Microsoft Windows XP Windows Vista Windows 7 Genie III PC PC Genie Explorer N Genie Explorer USB PC OS OptiGene Ltd http www optigene co uk support http nippongene analysis com genie3 fs htm Genie Explorer NET Frameworks 4 0 PC
7. LAMP Genie II e Genome Order e Genome Order http genome e mpjp Isothermal Master Mix for Genie II Isothermal Master Mix for Genie III 1 DNA MgCI dNTPs Buffer DNA Genie II Tube Strip Genie II Genie III Tube Strip Genie III Cooling Block Genie II Tube Strip
8. 22 Thermal Profile PreheaUActivation Temperature 4500 C Time 3 Minutes y Isothermal lemperature 65 00 PC Duration 30 Anneal Siart Temperature 9800 SO End Temperature 8000 gt ce Ramp Rate 0 05 5C Sec New Preheat Activation lsothermal Anneal C 23 Advanced a Advanced Preheat lsothermal Ramp rate h 00 Ramp rate 1 00 FCiSec Mid Point Temperature Measure period 15 Sec Block Gradient 0 Anneal Initial rate 100 CSec Setlingtme 60 Measure period 0
9. Isothermal Anneal Anneal Low Gain Gain Genie I Gain 0 Normal use 4 Low gain for Calcein Block Gradient Mid Point Temperature ee dn 0 s Mid Point Temperature 63 5 C Block Gradient 7C 63 5C 1 60 C 8 67 C Profile Genie Profile
10. Normalisation Normalisation Normalisation 31 Peak Detection SImple Threshold Full 32 V e Genome Order e Genome Order http genome e mpJp EE 200
11. FTemp c ET MT MT Extend Extend Ves 10 Advance IAdvance IYes Stop IStop es Amplification rs rm 9 Genie
12. http www microsoft com ja jp download details aspx 2id 17851 19 GenieInstallmsi The installer will guide unu through the steps required to install enie Explorer on your computer WARNING This computer program is protected by copyright law and international treaties Unauthorized duplication or distribution of this program or any portion of it may result in sewere ciwil or criminal penalties and will be prosecuted to the maximum extent possible under the law Genie Explorer EE Select Installation Folder Next gt gt The installer will install enie Explarer to the fallowina folder Ta install in this folder click Nest To install ta a different folder enter it belaw nr click Brawse Falder Cs Fragram Files Ontigene Genie Explorer jmstall Genie Explorer far yourself or for anyone who uses this computer Everyone Just me Genie Explorer Close Installation Complete
13. mr re 1 i oi hPa Pg __ _ IP
14. Amplification Anneal Amplification Anneal mmm ee Derivative FC CTI Results IGEN3 1013 0005 Isothermal Ratio Genie II deh Profie Results wel e Amplification mm ss Anneal C 11 15 84 39 wal WEN Isothermal Ratio qPCR Ct Cq
15. Genie II Run Genie III 1 A 24 Graph Options Genie II 25 4 Genie II Profile Profile
16. Genie I Version 2 0 NE5011 DD NE i ae _ a a _
17. 96 AIAIQAI IS IL3 Temperature _ Profile Amplification Amplification 7 26 Amplification rate Amplification rate shold Amplification rate Amplification Isothermal Ratio qPCR Ct Cq
18. 4 5 Cancel 07 04 2014 11 47 LAIMP amp ATneal prof 07 04 2014 11 47 start Edit Other 12
19. PC Genie Explorer Profile 10 1 Run profile txt jpg 29 ProflleName ww 1 1 TTYTESTT EmdTime ET Tr 2z01 fzs 000900 mm 1300 Mmo Mm Time hhimmias 2014 Z5 30 wax Seoe Time hh mm 58 2z0i zs 5 Smoothing Smoothing Points Normalisation Normalisation From 60 SecTo 180 BSE Smoothing Points
20. Genie III PC Genie II PROFILE SYSTEM 20 Genie Utilities Update TD MC MT A i Touchscreen Battery Test Time amp Date Update lOpen PROFILE FPGA Genie I OFF FPGA Genie II Open 21 3 Genie Explorer OptiGene Genie Explorer OptiGene 1 connected GEN3 1035 8 PC Genie Explorer Genie Explorer Genie II Genie II
21. PC ienie Explorer has heen successfully installed Click Close to exit Flease use Windows Update to check for any critical updates to the NET Framework Close 19 2 Genie III Genie II FPGA 2 LAMP amp Anneal nneal Other FPGA OptiGene OptiGene Ltd http www optigene co uk support zip mot FPGA rbf FPGA FPGA PC
22. OptiGene Manual OptiGene Ltd http www optigene co uk support http nippongene analysis com genie3 fs htm I II III 1 2 3 Genie II Genie I Genie II RR ROSS 7820 ESE 8 1 Genie III 2 3 4 RTP Pe 11 ON Genie II IV PO de OE ENS TS 18 V 1 Genie Explorer 2 Genie 3 Genie Explorer 4 5 OP 33 Genie II Version 2 0 Genie I
23. 750uLx4 Isothermal Master Mix for Genie II eos Pemaertintr me 400 750 uLx8 50 strips 50 strips x 1 NE5201 s II Tube Strip 6 NE5203 250 strips 50 strips x 5 NE5301 Genie I Cooling Block 1 33 34 DD OptiGene Ltd 2015 5 NIPPON GENE TEL 076 451 6548
24. Amplification Ratio Peak detect Simple Full Threshold Results 2 Anneal DNA Annealing curve Anneal Derivative Anneal Derivative 1 Results Results rs Amplification rate 28 IGG Il AIAIAI IS gen gen
25. Genie III 1 Genie I Genie II
26. 0 40C 20 70C 40 Wh 1 5 Co Genie II LAMP Loop mediated Isothermal Amplification DNA II Genie II ml Genie II Genie I
27. ON Genie LED Genie II OFF 78 Battery Status Cha dr 2841 mAh UV 2 SA ih 48mn achve tme LAMP amp Anneal 100 ali Battery Status Battery Ful Versoe 3 06 r 2 3033 MAhn Ei 16 TV 0 SN GEN3 1C29 1 4 LAMP amp Anneal Other USB serial Vaersion 3 06 rc 2 FPGA 406 SIN GEN3 1029 II Genie II
28. TEL 03 6 712 3885 FAX 076 451 6547 FAX 03 6712 3886 E mail support nippongene analysis com E mail feast egenome csjjp fujltsu com URL http nippongene analysis com URL http genome e mpjJp Copyright 2015 NIPPON GENE CO LTD All Rights Reserved
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