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Contents
1. ras Cc 2 o LAMP CBH3512. RAA 1 CBH351_J1_GG13FM 11 GM CBH351 1 DNA 1 1
3. CBH351 CBH351 ELISA Enzyme Linked Immunosorbent Assay PCR Polymerase Chain Reaction ELISA PCR DNA
4. DNA lt DNA gt DNeasy Plant Maxi Kit DNA 10 ng ul 1 mM EDTA 10 mM Tris HCI pH 80 B 65C
5. gt 20000ppm 2 D 70 1 D m PATAC
6. 65 C CBH351_J1_GG13FM 10 SERI DNA LAMP m LAMP DNA 2 2
7. LAMP B MSDS http nippongene analysis com Co 9 CBH351_J1_GG13FM 1 CTEIEZIESCSERERIZSUSLILLERTNIUILTIRUEIUDIUIALUVREUELEZSTILNMEUEEZITLTOTRZLEESITALTECNLE 4 GM
8. 3 2 UV CBH351 gt CBH351 CBH351 CBH351 10x CBH351 10x SSIlb
9. A A 254 366 nm UV 320 nm CBH351 J1 GG13FM 16 6 8 9 Dardenne F Seurinck J Lambert B Peferoen M 1990 Nucleotide sequence and deduced amino acid sequence of a cryIA c gene variant from Bacillus thuringiensis Nucleic Acids Res 18 18 5546 Lambert B Hofte H Annys K Jansens S Soetaert P Peferoen M 1992 Novel Bacillus thuringiensis insecticidal crystal protein with a silent activity against coleopteran
10. DNA 2 C EDTA D A
11. 1 X 3 2 3 1 5 ml 1 x 3 lt 1 2 5 ul 25 0 pl gt 1 2x DNA 12 5 ul 10x 2 5 ul 1 0 ul 1 0 ul 5 5 ul 22 5 ul 10x SSIb CBH351_J1_GG13FM 13 2 4
12. 1 22 5 ul 2 5 25 ul DNA 2 5 25 ul 200 ul 2 6 1 x3
13. NIPPON GENE TEL 076 442 3660 FAX 076 443 9120 E mail gmo nippongene analysis com URL http nippongene analysis com Copyright 2006 NIPPON GENE CO LTD All Rights Reserved
14. Starch CBH351_J1_GG13FM 3 JETER BASE e e 0 5 10 ul 10 100 ul 200 1 000 ul e 1 5 ml CBH351_J1_GG13FM e e UV 254 366 nm e 60 65 C 70 50 ml DNA CBH351_J1_GG13FM 4 10 GERD DNA 1 LAMP DNA 10x CBH351 10x
15. DNA 10x CBH351 CBH351_J1_GG13FM 15 5 A A B
16. 10x PM CBH351 1st 30 ul 20 C 10x SSIb 10x PM zSSIlb 60 ul 20 C 10x CBH351 10x PM CBH351 2nd Ba 30 ul 20 C HO 300 ul 20 C Synthase IIb DNA 20C 6 20C 4C CBH351 c 9
17. 65 C 1 CBH351_J1_GG13FM 14 3 3 1 f UV nl ee 80C 2 lt UV UV 254 366 nm UV i 80 C 1
18. DNA LAMP CBH351 DNA CBH351 CBH351 DNA DNA 60 65C 1 CBH351 DNA DNA DNA DNA CBH351 DNA CBH351 c 9 Starch Synt
19. CBH351 CBH351 LAMP Loop mediated isothermal amplification 2 Ke RON NOW AORCAIOWOR CA N XOW SON CRCUC NOR KOREA ONCE CC RONRCRCRTR VOR OA CRON a O O 2 5 48 3 TCI 6 4 E e E ET E N ESE D RT UR VRRRY A RR ra am OP UR de E D AT AR WP 8 GM CBH351 5 ennnnnnnnnnnnnnunnnnnnnnnnnnunnnnnnnnnnnnnnnnnnnnnnnn 16 CBH351_J1_GG13FM 1 w REREH GM CBH351 LAMP CBH351 StarLink CBH351 LAMP SARS
20. DNA 1 2 DNA DNA DNA DNeasy Plant Maxi Kit DNA 10 ng ul 1 mM EDTA 10 mM Tris HCI pH 8 0 CBH351_J1_GG13FM 12 2 2 1 DNA 10x 20 C 2 2
21. CBH351_J1_GG13FM 17 7 2 5 ul 25 ul 1 DNA 65 C 1 2 5 ul 25 ul 1 DNA 65 C 1 LAMP 4 6 10x SSIb DNA 2 5 pl 400 000 CBH351_J1_GG13FM 18 19 CBH351 J1 GG13FM i
22. PCR 2 DNA LAMP Loop mediated isothermal amplification LAMP DNA DNA LAMP Eiken GENOME SITE http loopamp eiken cojp CBH351_J1_GG13FM 2 48 E 1 48 2x DNA 2x RM 600 ul 20 C ES 48 ul 20 C FD 48 yl 20 C GEH PC 30 ul 20C MO 1 ml 20C 10x CBH351
23. SSilb 1 DNA 2 LAMP DNA 10x CBH351 10x SSilb 2 CBH351_J1_GG13FM 10 1 DNA 2 1 2x DNA 12 5 ul 10x 2 5 yl 1 0 ul 1 0 ul 5 5 ul 22 5 yl 25 ul 1 250 ul 0 5 1 3 22 5 4 2 5 ul gt 5 200 ul 8 6 65C
24. larvae App Environ Microbiol 58 8 2536 Lambert B Buysse L Decock C Jansens S Piens C Saey B Seurinck J Van Audenhove K Van Rie J Van Vliet A Peferoen M 1996 A Bacillus thuringiensis insecticidal crystal protein with a high activity against members of the family Noctuidae App Environ Microbiol 62 1 80 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA Nucleic Acids Res 28 12 e63 Prince AM Andrus L 1992 PCR how to kill unwanted DNA B otechniques 12 3 358 T Matsuoka H Kuribara K Takubo H Akiyama H Miura Y Goda Y Kusakabe K Isshiki M Toyoda and A Hino 2002 Detection of recombinant DNA segments introduced to genetically modified maize Zea mays J Agric Food Chem 50 7 2100 2001 CBH351 42 3 197 JAS 2 gt DNA
25. 1 7 80C 2 CBH351_J1_GG13FM m gt
26. LAMP GM CBH351 GM Maize Detection CBH351 Version 1 0 amp amp LAMP GM CBH351 version 1 0 GM CBH351 1 LAMP CBH351 2 20C 6
27. hase IIb CBH351 CBH351 ac s thuringiensis subspecies tolwothi cry9C cry9C CBH351 1
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